Rorug05G0200200

C2 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0006102
Physical Location & Seq
Forward (+)
19810223 .. 19826915
16693 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0200200.1

Sequence Viewer

Length: 5859 bp
ATGTCTGAGATTGTGGTTGCAGAGCCCATCTATGATGGTCGAGCAGAGGAAAAGAGACCCATTACAAGGTATCTCACCATGGGCAGTGGGAGTGGTCATAATCATCCTCCTGAGCCATTTGAGAGCGAGAGATTGCCTGCCACTTTGGCCTCAAAAATCCGGAGCTTTCTTCGGGTTGCAAACTTGATCGGAAGCGAGGAGCCTCGGATTGCTTATCTGTGCCGCTTTCATGCCTTTGAAATAGCACATAACATGGACCGTCATTCGAATGGGAGGGGTGTTCGACAATTCAAAACTACCCTTCTGCAGCGACTTGAACAGGATGAAGAAACTACAATTAGGAAACGGAAGGAAAAGAGTGATATACGCGAGTTAAGACGCGTTTATCATGCTTACAAGGAGTACATCATCAAACATGAAGGAGCATTTAGCATAGAAAATAGTCACAGAGAGAAGTTGATAAATGCGCGTATAATTGGTTCTGTATTGTTTGAAGTCTTAAAGACAGTTTCAAATACAGCCGGTCCTCAGGCCCTGGCTAATAGAGGTGGTATCCAGACAAAACCTTATGATATCTTTGGCATGCCATATAATATTCTACCACTGGATCAAGGAGGTATTCAGCAACCAATCATGCAGCTACCTGAGATTAAGGCGGCAGTAGCAGCAATACGGTATATTCGTGGCATACCTTCCAATGAAGATCTTCAGAAACATGGGACTTTCATTGATTTGTTTGATTTTCTTCAGTACTGCTTTGGATTTCAGGAAGGGAATGTAGCCAATCAGAGAGAGCATCTGCTTCTACTCCTTGCTAATATTCACAAACGGAAAACTCATAAACAGACATCTGTCTCAAAATTAGGAGATGCTGTAGTGGACGAGTTAATGCAGAGAATTTTTAAAAATTATACAAACTGGTGCAAGTTTTTGGGAAGGAAAAGCAACATCCGGTTACCTTATGTGAAACAAGAAGCTCAACAATATAAACTTCTATTTCTGGGGCTTTACCTTCTTATATGGGGCGAGGCTGCTAACTTGCGATTCATGCCAGAGTGTCTCTGTTATATTTTTCACCATATGGCATATGAGCTACATGGCATGTTAACTGGTGCTGTTAGCTTGACAACATGGGAGAAGGTCATGCCAGCATATGGAGGGCAGTCTGAGTCTTTCCTTAACAATGTTGTTACCCCCATATATGGGGTTATAAGAGAGGAAGCCGAGAAAAGCAAGTGTGGGACAGCTGACCATTCCACATGGAGAAACTATGATGATCTGAATGAGTATTTTTGGTCTCCTGATTGTTTTGAAATCGGTTGGCCCATGCGTCTAGATCACGATTTCTTCTTTACAGACTCTCCAACAAAATCCAATGCTAAGAATGCTTCAGCATCCACTGCTCCTGTAGAAGAAAGAAGGAAGGAAGATGAGGAGGAAGATGAAGTTGGGGTTACTAAAGAAGAAGTCCGTGAACCCAAATGGTTGGGTAAAACAAATTTCGTAGAAGTACGTTCGTTTTGGCAAATTTTTAGAAGCTTTGACAGAATGTGGAGCTTTTTTATTGTTTCACTTCAGGCATTAATAATTATGGCATGCCATGAATTGGAATCTCCACTTCAGTTGTTTGACAAAGTTATATTTGAGGATATCATGAGCATCTTCATTACATCTGCATTTCTTAAATTCATACAAGCAATACTCGACATTGCCTTTACATGGAAAGTGAGACAAACAATGGACTTCTCTGCAAAAGTAAAACATGTTATGAAGCTCAGTGTTGCAATGATATGGACTATAGTTCTTCCTGTATATTATGCTAATTCTAGAAGAAAATATACTTGTTATCCCACAACATACGGAAGCTGGGTTCAAGAATGGTGTTTCTCTTCCTTTATGGTTGCAGTTGCAATTTATTTGATGACTAATGCAGTTGAGATGGTGTTATTTTTGGTCCCTTCTATTCGCAAATACATTGAGGTCTCAAATTACCGAATATGCACCATTATGTCTTGGTGGACACAGCCTAGGTTATATGTTGCGCGTGGATTGCAAGAAAGCCAGCTTTCAGTGTTGAAGTATACATTGTTTTGGGTACTTGTACTATTGAGCAAATTTTCTTTCAGCTACTTTTTTGAGATAAAACCCCTTATAGAGCCAACAAAGCAGATCATGAAAATTGGTGTGCAAATGTATGATTGGCATGAGCTTTTTCCGAAAGTTAAGAGTAATGCTGGTGCGATTGCTGCTATATGGGCTCCAATTATAGTTGTGTACTTTATGGACACTCAGATATGGTATTCTGTTTTTTGCACAATCTTTGGTGGAGTATATGGCATTTTGCATCACCTTGGTGAGATTCGGACGTTGGGAATGTTGAGGAGTAGATTTCACTCCTTGCCTTCCGCATTCAATATTTCTCTTATTCCACCCTCATTGAGAAATGACCAAAAGAGAAGAAAAGGTTTCTTTTACAATAAATTCAGAAAGGTATCAAAGAGTGAAAAAAATGGTCTTGCCAAGTTTGTCTTGGTTTGGAACCAAATTATCAATAGTTTTCGATTGGAGGACTTGATAAACAACAGGGAGCTGGATTTAATGACAATGCCAATGTCTTCAGAATTATTTTCCGGCATAGTTCGTTGGCCAGTTTTTCTTCTTGCAAATAAGTTTTCAACTGCCCTGAGTATTGCAAAGGATTTTGTGGGGAGGGATGAAAGTCTTGTCAGAAAACTTAAAAAAGATGAGTACATGTACTGTGCTGTGAAAGAGTGCTACGAATCATTGAAGTACATCCTTGAAATCCTCATTATTGGGGATCTCGAAAAGAGGATTGTATCTGCCATACTGACTGAAATTGAAGAAAGCATCACAAGATCAAGTCTTCTTGAGGACTTTAGGATGATTAAAGTTCCAGATCTTTTGGCTAAATGTATCGAGCTAATTGAACTTTTGGTTGAGGGAAATGAAGACCATCATGGTAAAGTTGCGAAAATTCTCCAAGATATATTTGAGCTTGTAACCAATGATATGATGACGACTGGCTTCAGGATATTAGAGTTGCTGGATTCATCCCAACAGACCGAAACAGATGCTGCTTATTTTTCTGGAAGCATTGAATCCCCATTATTTGGATCTGCTGGTGGCAAGAATTCTATCCATTTCCCTTTGCCAGACAGTGCTGCTCTGAATGAGCAGATCAAGCGTTTTCTTCTGTTGCTTACTGTCCAAGATACAGCAATGGACATTCCAACTAACTTAGAGGCTCGGAGACGCATATCATTCTTTGCCACTTCTCTCTTTATGAATATGCCACATGCACCCACAGTGGCCAATATGGTGCCATTCAGTGTTATGACACCACATTACCTGGAGGATATTAATTTTTCAATGGAGGAGCTTCATTCAAGCCAGCGAGAAGTCTCTATCATCTTTTACATGCAAAAGATATTTCCAGATGAGTGGAAAAACTTCCTGGAGCGCATGGGTTATGAAAATTTGGATGGGTTGGAGAAGGAAAAAAAGGAAGACCTTAGAAATTGGGCTTCTTTCCGGGGCCAAACACTGAGCAGAACAGTTAGAGGAATGATGTACTATAGAGAAGCCTTAAAACTACAAGCATTTCTTGACATGGCTGAAGATGAAGATATTCTTGAAGGTTATGATGCTGTTGAAAGTAGAAATCATGCATTATCTGCTCAGCTAGATGCATTAGCAGACATGAAATTCACATATGTTGTTACCTGCCAATTATTTGGATCACAAAAAGCTGCTGGAGACCCACATGCACAGGACCTAATTGACTTGATGAAAAGATACCCAACTCTCCGTGTTGCTTACGTCGAGGAGAAAGAAGAGATAGTGGAGAACAAGCCTTGCAAAGTTTATTCTTCAGTATTGGTTAAAGCTATCCCTGATTTTGGTGATCAGGAAATATATCGCATAAAGCTTCCTGGTGCACCAACTATTGGAGAAGGGAAGCCCGAAAACCAGAACCATGGAATAATCTTCACTCGTGGTGAAGCTCTCCAAACCATTGATATGAACCAAGACAGTTATTTGGAAGAAGCATTCAAAATGAGAAATCTTCTGCAAGAATTTCTTCAGAACCGAGGACGGCGGCCTCCTATACTGCTTGGTTTGAGGGAACATATATTTACAGGAAGTGTTTCTTCTCTTGCATGGTTCATGTCTTATCAAGAGACCAGCTTTGTCACCATTGGTCAAAGGCTTCTTGCCAATCCTCTCAGGGTAAGATTCCACTATGGACATCCAGATGTATTTGACAGGTTATTCCACATCACAAGAGGTGGATTGAGCAAAGCATCAAAAACAATTAACCTGAGCGAGGATGTTTTTGCTGGGTATAATTCTACCTTACGACGGGGATGGATCACGCATCATGAATACATGCAAGTTGGCAAGGGTCGTGATGTAGGTCTAAACCAGATCTCAAAGTTTGAAGCTAAGGTTGCTAATGGTAACAGTGAACAAACTATAAGTCGTGACATATACCGCCTTGGACGTCAGTTTGATTTCTTCAGGATGCTGTCTTGTTATTTCACAACCATTGGGTTTTACTTTAGCAGCCTGATATCAGTTATTGGAATCTATGTGTTCCTTTACGGACAGCTATACCTAGTTCTCAGTGGCTTGGAAAAAGCTCTCGTAATTGAGGCTAGACTGCAGAACATACAATCCTTGGAAACAGCTCTTGCCTCCCAGTCATTTATACAGCTTGGGCTATTAACAGGAATGCCAATGGTGATGGAAATTGGACTTGAGAAAGGGTTCCTCAATGCACTAAAAGATTTTGTCCTGATGCAATTGCAGCTGGCTTCTGTTTTTTTCACGTTCTCCTTTGGAACAAAAATCCATTATTATGGCCGAACAATTCTGCATGGTGGTGCTAAATACAGACCCACTGGGCGTAAGGTTGTTGTGTTTCATACTAGCTTCACTGAAAACTACAGGTTGTATTCAAGAAGCCACTTCGTCAAAGGATTTGAGTTACTGCTCCTGTTGATTGTTTATAACTTGTTTAGACGGTCCTATGAGAGCGGCATGGCATATGTACTAATCACTTATTCAATTTGGTTCATGTCAATCACTTGGTTGTTCGCTCCATTTCTTTTTAATCCGTCTGGATTCAGTTGGGACAAGATAGTAGATGACTGGAAAGATTGGAATAAGTGGATCAGGCAGCAGGGTGGTATTGGAGTTCACCAAGATAAAAGTTGGCAGTCTTGGTGGATAGATGAGCAGGACCATCTTCGTCATTCTGGGATGAGTTCTAGGTTGTTTGAAATACTCCTTTCTGTTCGGTTTTTCCTGTATCAGTATGGTCTGGTCTACCACCTTGACATTTCCCAGAATAGCAGGAATTTTCTGGTTTATTTGCTTTCATGGATAGTGATCCTCGTTGTTTTCTTGTTAGTAAAGGCAGTGAACCTCGGAAGGCAACGGTTCAGTGCTAGACATCACCTTTGGTTTAGGATATTCAAAGCAACCCTCTTCTTAGGTGTTCTTTCCATCATAATATCTCTCTCTCTTGTGTATCATCTATCTTGGATGGACTTGGTTGTGTGCTGCCTAGCATTTTTGCCAACAGGTTGGGGTTTGATATTGTTTGCGCAAGCTGTGAGGCCCCTGATAGAGAGTACTGGATTGTGGGAATTCACCAGAGTACTTGCTAAAACATATGACTATGGAATGGGGGTTGTTCTCTTTACACCTATTGCTGTCTTGGCATGGCTTCCGATTATATCAGCATTCCAGACTCGATTCCTGTTCAACGAGGCCTTCAATAGACACTTGCAGATCCAACCGATTCTTCAGGGGAAGAAGAAGAACAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

1952

Amino Acids

226.01

Weight (kDa)

8.61

Isoelectric Point (pI)

44.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Vta1 PF04652 53 - 112 2.7e-07 Vta1 like
FKS1_dom1 PF14288 330 - 441 4.1e-42 1,3-beta-glucan synthase subunit FKS1, domain-1
CALS1 PF25968 883 - 1076 1.9e-47 Callose synthase 1 domain
Glucan_synthase PF02364 1083 - 1166 1.5e-24 1,3-beta-glucan synthase component
Glucan_synthase PF02364 1171 - 1769 7.3e-196 1,3-beta-glucan synthase component
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1211, 5034
AatII GACGTC 1 cut(s) 4528
Acc16I TGCGCA 1 cut(s) 5634
Acc36I ACCTGC 1 cut(s) 3752
AccB1I GGYRCC 1 cut(s) 3340
AccB7I CCANNNNNTGG 4 cut(s) 1154, 1606, 3131, 3968
AccBSI CCGCTC 1 cut(s) 5061
AccI GTMKAC 2 cut(s) 2081, 5352
AccII CGCG 4 cut(s) 369, 381, 469, 2044
AccIII TCCGGA 1 cut(s) 159
AciI CCGC 6 cut(s) 223, 656, 2406, 4120, 4516, 5061
AclWI GGATC 8 cut(s) 615, 2826, 3142, 3766, 4400, 5204, 5410, 5815
AcoI YGGCCR 3 cut(s) 2645, 3330, 4885
AcyI GRCGYC 1 cut(s) 4525
AflIII ACRYGT 3 cut(s) 379, 1762, 2751
AjnI CCWGG 4 cut(s) 534, 3369, 3474, 3952
AjuI GAANNNNNNNTTGG 4 cut(s) 1431, 1463, 5817, 5849
AleI CACNNNNGTG 1 cut(s) 2352
AloI GAACNNNNNNTCC 4 cut(s) 1854, 1886, 4682, 4714
Alw21I GWGCWC 1 cut(s) 3961
Alw44I GTGCAC 1 cut(s) 3957
AlwI GGATC 8 cut(s) 615, 2826, 3142, 3766, 4400, 5204, 5410, 5815
AlwNI CAGNNNCTG 2 cut(s) 535, 3095
Aor13HI TCCGGA 1 cut(s) 159
ApaLI GTGCAC 1 cut(s) 3957
ArsI GACNNNNNNTTYG 4 cut(s) 4486, 4518, 5469, 5501
AseI ATTAAT 2 cut(s) 1583, 3381
Asp700I GAANNNNTTC 6 cut(s) 705, 3470, 3648, 4101, 4168, 4790
AspA2I CCTAGG 1 cut(s) 2027
AspLEI GCGC 4 cut(s) 469, 2044, 3483, 5635
AsuC2I CCSGG 1 cut(s) 3554
AsuII TTCGAA 1 cut(s) 266
AvaII GGWCC 6 cut(s) 256, 524, 1954, 3793, 5049, 5266
AvrII CCTAGG 1 cut(s) 2027
AxyI CCTNAGG 1 cut(s) 528
BaeGI GKGCMC 1 cut(s) 3961
BalI TGGCCA 2 cut(s) 2647, 3332
BanI GGYRCC 1 cut(s) 3340
BanII GRGCYC 2 cut(s) 27, 2260
BarI GAAGNNNNNNTAC 2 cut(s) 1822, 1854
BauI CACGAG 1 cut(s) 4014
BbsI GAAGAC 4 cut(s) 2607, 2876, 2976, 3534
Bbv12I GWGCWC 1 cut(s) 3961
BceAI ACGGC 1 cut(s) 4133
BcgI CGANNNNNNTGC 2 cut(s) 3074, 3108
BciT130I CCWGG 4 cut(s) 536, 3371, 3476, 3954
BciVI GTATCC 1 cut(s) 563
BclI TGATCA 1 cut(s) 3925
BcnI CCSGG 1 cut(s) 3554
BfmI CTRYAG 7 cut(s) 305, 873, 1407, 1797, 3595, 4685, 4969
BfuAI ACCTGC 1 cut(s) 3752
BfuI GTATCC 1 cut(s) 563
BglI GCCNNNNNGGC 1 cut(s) 146
BglII AGATCT 3 cut(s) 703, 2917, 4449
BlnI CCTAGG 1 cut(s) 2027
BlpI GCTNAGC 1 cut(s) 3699
BmcAI AGTACT 3 cut(s) 752, 5662, 5688
Bme1390I CCNGG 5 cut(s) 536, 3371, 3476, 3554, 3954
Bme18I GGWCC 6 cut(s) 256, 524, 1954, 3793, 5049, 5266
BmiI GGNNCC 8 cut(s) 201, 1956, 2259, 2540, 3342, 3557, 4793, 5648
BmrFI CCNGG 5 cut(s) 536, 3371, 3476, 3554, 3954
BmrI ACTGGG 2 cut(s) 4717, 4935
BmuI ACTGGG 2 cut(s) 4717, 4935
BoxI GACNNNNGTC 1 cut(s) 851
BpiI GAAGAC 4 cut(s) 2607, 2876, 2976, 3534
BpmI CTGGAG 3 cut(s) 3392, 3497, 3795
Bpu10I CCTNAGC 3 cut(s) 111, 4343, 4467
Bpu1102I GCTNAGC 1 cut(s) 3699
Bpu14I TTCGAA 1 cut(s) 266
BpuEI CTTGAG 2 cut(s) 2909, 4802
BpuMI CCSGG 1 cut(s) 3554
BsaBI GATNNNNATC 1 cut(s) 5414
BsaHI GRCGYC 1 cut(s) 4525
BsaI GGTCTC 5 cut(s) 49, 1302, 1987, 3771, 4196
BsaWI WCCGGW 2 cut(s) 159, 951
BsaXI ACNNNNNCTCC 4 cut(s) 858, 888, 1345, 1375
Bse118I RCCGGY 1 cut(s) 521
Bse1I ACTGG 9 cut(s) 609, 923, 1114, 2648, 3046, 4723, 4930, 5180, 5668
Bse21I CCTNAGG 1 cut(s) 528
Bse3DI GCAATG 3 cut(s) 1707, 1791, 3246
Bse8I GATNNNNATC 1 cut(s) 5414
BseAI TCCGGA 1 cut(s) 159
BseBI CCWGG 4 cut(s) 536, 3371, 3476, 3954
BseJI GATNNNNATC 1 cut(s) 5414
BseMI GCAATG 3 cut(s) 1707, 1791, 3246
BseNI ACTGG 9 cut(s) 609, 923, 1114, 2648, 3046, 4723, 4930, 5180, 5668
BseRI GAGGAG 5 cut(s) 212, 1448, 2395, 3410, 3860
BseSI GKGCMC 1 cut(s) 3961
BseYI CCCAGC 2 cut(s) 1866, 4361
Bsh1236I CGCG 4 cut(s) 369, 381, 469, 2044
BshNI GGYRCC 1 cut(s) 3340
BsiHKAI GWGCWC 1 cut(s) 3961
BsiSI CCGG 5 cut(s) 160, 522, 952, 2631, 3553
BslFI GGGAC 4 cut(s) 733, 1255, 1940, 5171
BsmBI CGTCTC 1 cut(s) 3265
BsmFI GGGAC 4 cut(s) 733, 1255, 1940, 5171
BsmI GAATGC 5 cut(s) 1390, 2408, 4070, 4761, 5771
Bso31I GGTCTC 5 cut(s) 49, 1302, 1987, 3771, 4196
Bsp119I TTCGAA 1 cut(s) 266
Bsp1286I GDGCHC 3 cut(s) 27, 2260, 3961
Bsp13I TCCGGA 1 cut(s) 159
Bsp1720I GCTNAGC 1 cut(s) 3699
Bsp19I CCATGG 2 cut(s) 78, 3997
BspACI CCGC 6 cut(s) 223, 656, 2406, 4120, 4516, 5061
BspEI TCCGGA 1 cut(s) 159
BspFNI CGCG 4 cut(s) 369, 381, 469, 2044
BspHI TCATGA 3 cut(s) 1653, 2172, 4402
BspLI GGNNCC 8 cut(s) 201, 1956, 2259, 2540, 3342, 3557, 4793, 5648
BspMAI CTGCAG 2 cut(s) 309, 4689
BspMI ACCTGC 1 cut(s) 3752
BspPI GGATC 8 cut(s) 615, 2826, 3142, 3766, 4400, 5204, 5410, 5815
BspT104I TTCGAA 1 cut(s) 266
BspT107I GGYRCC 1 cut(s) 3340
BspTNI GGTCTC 5 cut(s) 49, 1302, 1987, 3771, 4196
BsrBI CCGCTC 1 cut(s) 5061
BsrDI GCAATG 3 cut(s) 1707, 1791, 3246
BsrFI RCCGGY 1 cut(s) 521
BsrI ACTGG 9 cut(s) 609, 923, 1114, 2648, 3046, 4723, 4930, 5180, 5668
BssAI RCCGGY 1 cut(s) 521
BssNAI GTATAC 1 cut(s) 2082
BssNI GRCGYC 1 cut(s) 4525
BssSI CACGAG 1 cut(s) 4014
BssT1I CCWWGG 6 cut(s) 78, 2027, 2350, 3997, 4519, 4701
Bst1107I GTATAC 1 cut(s) 2082
Bst2BI CACGAG 1 cut(s) 4014
Bst2UI CCWGG 4 cut(s) 536, 3371, 3476, 3954
Bst6I CTCTTC 3 cut(s) 1894, 3849, 5519
BstACI GRCGYC 1 cut(s) 4525
BstAPI GCANNNNNTGC 2 cut(s) 1400, 3695
BstBI TTCGAA 1 cut(s) 266
BstC8I GCNNGC 8 cut(s) 138, 584, 1149, 1597, 2063, 3413, 4836, 5637
BstDSI CCRYGG 2 cut(s) 78, 3997
BstEII GGTNACC 1 cut(s) 954
BstENI CCTNNNNNAGG 1 cut(s) 4346
BstFNI CGCG 4 cut(s) 369, 381, 469, 2044
BstHHI GCGC 4 cut(s) 469, 2044, 3483, 5635
BstNI CCWGG 4 cut(s) 536, 3371, 3476, 3954
BstNSI RCATGY 9 cut(s) 586, 1105, 1599, 1766, 2755, 3320, 3442, 3788, 4414
BstPAI GACNNNNGTC 1 cut(s) 851
BstPI GGTNACC 1 cut(s) 954
BstSCI CCNGG 5 cut(s) 534, 3369, 3474, 3552, 3952
BstSFI CTRYAG 7 cut(s) 305, 873, 1407, 1797, 3595, 4685, 4969
BstSLI GKGCMC 1 cut(s) 3961
BstUI CGCG 4 cut(s) 369, 381, 469, 2044
BstV2I GAAGAC 4 cut(s) 2607, 2876, 2976, 3534
BstX2I RGATCY 6 cut(s) 703, 2818, 2917, 3134, 4449, 5820
BstXI CCANNNNNNTGG 6 cut(s) 1486, 3462, 3755, 3998, 4883, 5613
BstYI RGATCY 6 cut(s) 703, 2818, 2917, 3134, 4449, 5820
BstZ17I GTATAC 1 cut(s) 2082
Bsu36I CCTNAGG 1 cut(s) 528
BsuI GTATCC 1 cut(s) 563
BtgI CCRYGG 2 cut(s) 78, 3997
BtsI GCAGTG 3 cut(s) 91, 1398, 5451
BveI ACCTGC 1 cut(s) 3752
Cac8I GCNNGC 8 cut(s) 138, 584, 1149, 1597, 2063, 3413, 4836, 5637
CaiI CAGNNNCTG 2 cut(s) 535, 3095
CciI TCATGA 3 cut(s) 1653, 2172, 4402
CfoI GCGC 4 cut(s) 469, 2044, 3483, 5635
Cfr10I RCCGGY 1 cut(s) 521
CseI GACGC 3 cut(s) 387, 1319, 3282
CspCI CAANNNNNGTGG 4 cut(s) 1605, 1640, 2418, 2453
DraI TTTAAA 1 cut(s) 904
EaeI YGGCCR 3 cut(s) 2645, 3330, 4885
Eam1104I CTCTTC 3 cut(s) 1894, 3849, 5519
EarI CTCTTC 3 cut(s) 1894, 3849, 5519
Eco130I CCWWGG 6 cut(s) 78, 2027, 2350, 3997, 4519, 4701
Eco147I AGGCCT 1 cut(s) 5801
Eco24I GRGCYC 2 cut(s) 27, 2260
Eco31I GGTCTC 5 cut(s) 49, 1302, 1987, 3771, 4196
Eco32I GATATC 3 cut(s) 574, 1651, 4596
Eco47I GGWCC 6 cut(s) 256, 524, 1954, 3793, 5049, 5266
Eco81I CCTNAGG 1 cut(s) 528
Eco91I GGTNACC 1 cut(s) 954
EcoNI CCTNNNNNAGG 1 cut(s) 4346
EcoO109I RGGNCCY 3 cut(s) 532, 3793, 5646
EcoO65I GGTNACC 1 cut(s) 954
EcoRI GAATTC 2 cut(s) 3151, 5675
EcoRII CCWGG 4 cut(s) 534, 3369, 3474, 3952
EcoRV GATATC 3 cut(s) 574, 1651, 4596
EcoT14I CCWWGG 6 cut(s) 78, 2027, 2350, 3997, 4519, 4701
EcoT22I ATGCAT 2 cut(s) 3691, 3712
EcoT38I GRGCYC 2 cut(s) 27, 2260
ErhI CCWWGG 6 cut(s) 78, 2027, 2350, 3997, 4519, 4701
Esp3I CGTCTC 1 cut(s) 3265
FaqI GGGAC 4 cut(s) 733, 1255, 1940, 5171
FauNDI CATATG 6 cut(s) 1080, 1087, 1153, 3733, 5071, 5701
FbaI TGATCA 1 cut(s) 3925
FblI GTMKAC 2 cut(s) 2081, 5352
FriOI GRGCYC 2 cut(s) 27, 2260
FspI TGCGCA 1 cut(s) 5634
GlaI GCGC 4 cut(s) 468, 2043, 3482, 5634
GsaI CCCAGC 2 cut(s) 1870, 4365
GsuI CTGGAG 3 cut(s) 3392, 3497, 3795
HapII CCGG 5 cut(s) 160, 522, 952, 2631, 3553
HgaI GACGC 3 cut(s) 387, 1319, 3282
HhaI GCGC 4 cut(s) 469, 2044, 3483, 5635
Hin1I GRCGYC 1 cut(s) 4525
Hin6I GCGC 4 cut(s) 467, 2042, 3481, 5633
HinP1I GCGC 4 cut(s) 467, 2042, 3481, 5633
HincII GTYRAC 1 cut(s) 1107
HindII GTYRAC 1 cut(s) 1107
HindIII AAGCTT 2 cut(s) 1537, 3947
HpaI GTTAAC 1 cut(s) 1107
HpaII CCGG 5 cut(s) 160, 522, 952, 2631, 3553
Hpy99I CGWCG 2 cut(s) 3845, 4386
HpyCH4IV ACGT 5 cut(s) 1513, 2366, 3840, 4525, 4853
HpySE526I ACGT 5 cut(s) 1513, 2366, 3840, 4525, 4853
Hsp92I GRCGYC 1 cut(s) 4525
HspAI GCGC 4 cut(s) 467, 2042, 3481, 5633
Kpn2I TCCGGA 1 cut(s) 159
Ksp22I TGATCA 1 cut(s) 3925
KspAI GTTAAC 1 cut(s) 1107
MaeII ACGT 5 cut(s) 1513, 2366, 3840, 4525, 4853
MbiI CCGCTC 1 cut(s) 5061
MfeI CAATTG 1 cut(s) 4826
MflI RGATCY 6 cut(s) 703, 2818, 2917, 3134, 4449, 5820
MhlI GDGCHC 3 cut(s) 27, 2260, 3961
MlsI TGGCCA 2 cut(s) 2647, 3332
MluI ACGCGT 1 cut(s) 379
MluNI TGGCCA 2 cut(s) 2647, 3332
MlyI GAGTC 3 cut(s) 1178, 1352, 5773
MmeI TCCRAC 4 cut(s) 1388, 3275, 3489, 5848
Mox20I TGGCCA 2 cut(s) 2647, 3332
Mph1103I ATGCAT 2 cut(s) 3691, 3712
MroI TCCGGA 1 cut(s) 159
MroXI GAANNNNTTC 6 cut(s) 705, 3470, 3648, 4101, 4168, 4790
MscI TGGCCA 2 cut(s) 2647, 3332
MslI CAYNNNNRTG 7 cut(s) 267, 2006, 2352, 4040, 4275, 4881, 4905
Msp20I TGGCCA 2 cut(s) 2647, 3332
MspA1I CMGCKG 2 cut(s) 1247, 4834
MspI CCGG 5 cut(s) 160, 522, 952, 2631, 3553
MspR9I CCNGG 5 cut(s) 536, 3371, 3476, 3554, 3954
MunI CAATTG 1 cut(s) 4826
Mva1269I GAATGC 5 cut(s) 1390, 2408, 4070, 4761, 5771
MvaI CCWGG 4 cut(s) 536, 3371, 3476, 3954
MvnI CGCG 4 cut(s) 369, 381, 469, 2044
NciI CCSGG 1 cut(s) 3554
NcoI CCATGG 2 cut(s) 78, 3997
NdeI CATATG 6 cut(s) 1080, 1087, 1153, 3733, 5071, 5701
NlaIV GGNNCC 8 cut(s) 201, 1956, 2259, 2540, 3342, 3557, 4793, 5648
NmeAIII GCCGAG 1 cut(s) 1249
NmuCI GTSAC 3 cut(s) 443, 4213, 4505
NsbI TGCGCA 1 cut(s) 5634
NsiI ATGCAT 2 cut(s) 3691, 3712
NspI RCATGY 9 cut(s) 586, 1105, 1599, 1766, 2755, 3320, 3442, 3788, 4414
NspV TTCGAA 1 cut(s) 266
OliI CACNNNNGTG 1 cut(s) 2352
PaeI GCATGC 2 cut(s) 586, 1599
PagI TCATGA 3 cut(s) 1653, 2172, 4402
PceI AGGCCT 1 cut(s) 5801
PciI ACATGT 2 cut(s) 1762, 2751
PcsI WCGNNNNNNNCGW 1 cut(s) 679
PctI GAATGC 5 cut(s) 1390, 2408, 4070, 4761, 5771
PdmI GAANNNNTTC 6 cut(s) 705, 3470, 3648, 4101, 4168, 4790
PflMI CCANNNNNTGG 4 cut(s) 1154, 1606, 3131, 3968
PfoI TCCNGGA 1 cut(s) 3474
PleI GAGTC 3 cut(s) 1177, 1352, 5773
PpsI GAGTC 3 cut(s) 1177, 1352, 5773
PpuMI RGGWCCY 1 cut(s) 3793
PscI ACATGT 2 cut(s) 1762, 2751
PshAI GACNNNNGTC 1 cut(s) 851
PshBI ATTAAT 2 cut(s) 1583, 3381
PsiI TTATAA 2 cut(s) 1211, 5034
Psp5II RGGWCCY 1 cut(s) 3793
Psp6I CCWGG 4 cut(s) 534, 3369, 3474, 3952
PspEI GGTNACC 1 cut(s) 954
PspFI CCCAGC 2 cut(s) 1866, 4361
PspGI CCWGG 4 cut(s) 534, 3369, 3474, 3952
PspN4I GGNNCC 8 cut(s) 201, 1956, 2259, 2540, 3342, 3557, 4793, 5648
PspPPI RGGWCCY 1 cut(s) 3793
PsrI GAACNNNNNNTAC 2 cut(s) 463, 495
PstI CTGCAG 2 cut(s) 309, 4689
PstNI CAGNNNCTG 2 cut(s) 535, 3095
PsuI RGATCY 6 cut(s) 703, 2818, 2917, 3134, 4449, 5820
PvuII CAGCTG 2 cut(s) 1247, 4834
RseI CAYNNNNRTG 7 cut(s) 267, 2006, 2352, 4040, 4275, 4881, 4905
ScaI AGTACT 3 cut(s) 752, 5662, 5688
SchI GAGTC 3 cut(s) 1178, 1352, 5773
ScrFI CCNGG 5 cut(s) 536, 3371, 3476, 3554, 3954
SduI GDGCHC 3 cut(s) 27, 2260, 3961
SfcI CTRYAG 7 cut(s) 305, 873, 1407, 1797, 3595, 4685, 4969
SfuI TTCGAA 1 cut(s) 266
SinI GGWCC 6 cut(s) 256, 524, 1954, 3793, 5049, 5266
SmiMI CAYNNNNRTG 7 cut(s) 267, 2006, 2352, 4040, 4275, 4881, 4905
SmlI CTYRAG 2 cut(s) 2888, 4781
SmoI CTYRAG 2 cut(s) 2888, 4781
SphI GCATGC 2 cut(s) 586, 1599
SseBI AGGCCT 1 cut(s) 5801
SsiI CCGC 6 cut(s) 223, 656, 2406, 4120, 4516, 5061
SspI AATATT 3 cut(s) 595, 820, 2416
StuI AGGCCT 1 cut(s) 5801
StyD4I CCNGG 5 cut(s) 534, 3369, 3474, 3552, 3952
StyI CCWWGG 6 cut(s) 78, 2027, 2350, 3997, 4519, 4701
TaiI ACGT 5 cut(s) 1516, 2369, 3843, 4528, 4856
TaqI TCGA 9 cut(s) 40, 266, 283, 1704, 2560, 2823, 2937, 3843, 5782
TaqII GACCGA 1 cut(s) 3098
TauI GCSGC 4 cut(s) 225, 659, 4123, 5064
TseFI GTSAC 3 cut(s) 443, 4213, 4505
Tsp45I GTSAC 3 cut(s) 443, 4213, 4505
TspGWI ACGGA 7 cut(s) 361, 844, 1460, 1875, 3818, 4641, 5130
Van91I CCANNNNNTGG 4 cut(s) 1154, 1606, 3131, 3968
VneI GTGCAC 1 cut(s) 3957
VpaK11BI GGWCC 6 cut(s) 256, 524, 1954, 3793, 5049, 5266
VspI ATTAAT 2 cut(s) 1583, 3381
XagI CCTNNNNNAGG 1 cut(s) 4346
XbaI TCTAGA 2 cut(s) 1333, 1826
XceI RCATGY 9 cut(s) 586, 1105, 1599, 1766, 2755, 3320, 3442, 3788, 4414
XcmI CCANNNNNNNNNTGG 1 cut(s) 2527
XmaJI CCTAGG 1 cut(s) 2027
XmiI GTMKAC 2 cut(s) 2081, 5352
XmnI GAANNNNTTC 6 cut(s) 705, 3470, 3648, 4101, 4168, 4790
ZraI GACGTC 1 cut(s) 4526
ZrmI AGTACT 3 cut(s) 752, 5662, 5688
Zsp2I ATGCAT 2 cut(s) 3691, 3712
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.