MD03G1189600.v1.1

C2 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0006102
Physical Location & Seq
Reverse (-)
25995970 .. 25998656
2687 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1189600.v1.1.491

Sequence Viewer

Length: 1233 bp
ATGGATTCTCCTCAATCTGTTGTGTCTCCATTCAAAAGCTCTGTTGCCGAGCCTGAGAAGCAGTTCATTTGTACCAATGGGCCTGTATCCAATGGAACTGATGCCTATACAGATAGTAACTCAGAGAACTTCATTGGTGTTCTCGAGGTTTACGTTCATCAGGCTAGGGACATCCAGAACATCTGCATATACCACAAGCAAGATGTCTATGCTAAGCTATGCCTGACGTGCGATCCTGAAAAAACAGTCTCCACCAAGACCATTAACGGTGGTGGTAGAAATCCAGTCTTCAATGATAATGTCCAGCTCAATGTTCGGACTGTTGATTCCTCCCTCAAATGCGAGATTTGGATGCTGAGCAGGGTCAAGAATTATCTTCAAGATCAGTTGCTGGGGTTTTCTTTGGTGCCTTTGTCAGATGTCCTCATTAAGAACGGGAAGTTAGAGAAAGAGTTCTCTCTTTCTTCAACTGATCTGTTTCATTCCCCAGCAGGGTTTGTCCAGTTGTCCCTCTCGTATGCTGGAGATCTGCCAGAAGTAATCTCCCTGGCCTCAAATCCTATTGTGCAGGACTCGGAGATAGCTGAGTCATGTGAATTAGATAGGTTAGAGTTCCCAGATCCAAACATTGACAATGAAGACCAGAGGATGGTTTCAGAGTACATTAAGATCCCATGTTCTGAGTTTGAGTCTCAAAGCTCTGAGAGCTTTGTCACGGCTGACACTGAAACTCTGCCTAGTTCCGAAGTGGGTGTTCATGCTGTAGAAAGCTTGTCGACTGCTACTATTGAGTCTGCTCATGTTCGGAAGCTTGATTCTCCCCCAAGCAGCGTGTCAACTAATGGTGTATCATCTGCATCGGGTCATACAAGCTCAGAGTCATATGATGCTCCATCAGCTTTGAAATCTCCAAATCACGAACAAGTTTCAGCTTTGGAAGAGAAGAAAGTGGATAAAGATGGTGAGATTAATTCGTCTGATGCGGTGCCAAGTGATACATTTGAGAAGCCTACTGTCACAGTCACCGTTCCAGAGCATACGGCGGTGCAGCAAGATTTTGTAGACATGTACATGAAAAGCATGCAGCAATTCACTGAATCTTTGGCAAAAATGAAGCTTCCTTTGGACCTTGAATCACCAACCAGTTCAGGAAACTCGAGCACTGATCAGAAAATAGAGACACCAAAGAGCAGTGGTTCCCGTGTGTTTTACGGTAGTAGAGCTTTCTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

411

Amino Acids

44.79

Weight (kDa)

4.57

Isoelectric Point (pI)

56.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 46 - 149 1.3e-09 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 406, 985
AccB7I CCANNNNNTGG 1 cut(s) 649
AccI GTMKAC 2 cut(s) 776, 1062
AciI CCGC 2 cut(s) 983, 1043
AclWI GGATC 3 cut(s) 227, 614, 664
AfaI GTAC 3 cut(s) 73, 662, 1070
AfiI CCNNNNNNNGG 2 cut(s) 492, 649
AflIII ACRYGT 1 cut(s) 1065
AgsI TTSAA 6 cut(s) 34, 292, 380, 468, 904, 1133
AjiI CACGTC 1 cut(s) 228
AjnI CCWGG 1 cut(s) 546
AjuI GAANNNNNNNTTGG 2 cut(s) 1106, 1138
Alw21I GWGCWC 1 cut(s) 1163
Alw26I GTCTC 4 cut(s) 30, 253, 696, 1172
AlwI GGATC 3 cut(s) 227, 614, 664
AlwNI CAGNNNCTG 1 cut(s) 391
Ama87I CYCGRG 2 cut(s) 143, 1156
AoxI GGCC 2 cut(s) 80, 549
ApeKI GCWGC 3 cut(s) 828, 1048, 1084
AseI ATTAAT 1 cut(s) 969
Asp700I GAANNNNTTC 2 cut(s) 62, 452
AspS9I GGNCC 2 cut(s) 80, 1126
AsuHPI GGTGA 3 cut(s) 974, 1015, 1128
AvaI CYCGRG 2 cut(s) 143, 1156
AvaII GGWCC 1 cut(s) 1126
BanI GGYRCC 2 cut(s) 406, 985
BbsI GAAGAC 2 cut(s) 280, 645
Bbv12I GWGCWC 1 cut(s) 1163
BbvI GCAGC 3 cut(s) 840, 1060, 1096
BccI CCATC 3 cut(s) 643, 901, 953
BceAI ACGGC 2 cut(s) 732, 1056
BciT130I CCWGG 1 cut(s) 548
BciVI GTATCC 1 cut(s) 97
BclI TGATCA 1 cut(s) 1165
BcoDI GTCTC 4 cut(s) 30, 253, 696, 1172
BfaI CTAG 2 cut(s) 165, 738
BfmI CTRYAG 1 cut(s) 762
BfuI GTATCC 1 cut(s) 97
BglII AGATCT 1 cut(s) 526
BisI GCNGC 3 cut(s) 829, 1049, 1085
BlpI GCTNAGC 2 cut(s) 213, 356
BlsI GCNGC 3 cut(s) 830, 1050, 1086
Bme1390I CCNGG 1 cut(s) 548
Bme18I GGWCC 1 cut(s) 1126
BmeT110I CYCGRG 2 cut(s) 143, 1156
BmgBI CACGTC 1 cut(s) 228
BmgT120I GGNCC 2 cut(s) 80, 1126
BmiI GGNNCC 3 cut(s) 408, 987, 1198
BmrFI CCNGG 1 cut(s) 548
BmsI GCATC 5 cut(s) 91, 342, 866, 877, 970
BpiI GAAGAC 2 cut(s) 280, 645
BpmI CTGGAG 1 cut(s) 543
Bpu1102I GCTNAGC 2 cut(s) 213, 356
BsaJI CCNNGG 1 cut(s) 546
Bsc4I CCNNNNNNNGG 2 cut(s) 492, 649
Bse1I ACTGG 3 cut(s) 284, 502, 1143
BseBI CCWGG 1 cut(s) 548
BseDI CCNNGG 1 cut(s) 546
BseGI GGATG 3 cut(s) 171, 357, 654
BseLI CCNNNNNNNGG 2 cut(s) 492, 649
BseMII CTCAG 7 cut(s) 45, 135, 347, 576, 672, 693, 888
BseNI ACTGG 3 cut(s) 284, 502, 1143
BseXI GCAGC 3 cut(s) 840, 1060, 1096
BseYI CCCAGC 2 cut(s) 391, 487
BsgI GTGCAG 2 cut(s) 587, 1067
BshFI GGCC 2 cut(s) 82, 551
BshNI GGYRCC 2 cut(s) 406, 985
BsiHKAI GWGCWC 1 cut(s) 1163
BsiHKCI CYCGRG 2 cut(s) 143, 1156
BslFI GGGAC 2 cut(s) 182, 493
BslI CCNNNNNNNGG 2 cut(s) 492, 649
BsmAI GTCTC 4 cut(s) 30, 253, 696, 1172
BsmFI GGGAC 2 cut(s) 182, 493
BsnI GGCC 2 cut(s) 82, 551
BsoBI CYCGRG 2 cut(s) 143, 1156
Bsp1286I GDGCHC 1 cut(s) 1163
Bsp1407I TGTACA 1 cut(s) 1068
Bsp143I GATC 7 cut(s) 232, 382, 472, 526, 619, 669, 1165
Bsp1720I GCTNAGC 2 cut(s) 213, 356
BspACI CCGC 2 cut(s) 983, 1043
BspANI GGCC 2 cut(s) 82, 551
BspCNI CTCAG 7 cut(s) 46, 134, 348, 577, 673, 694, 887
BspLI GGNNCC 3 cut(s) 408, 987, 1198
BspPI GGATC 3 cut(s) 227, 614, 664
BspT107I GGYRCC 2 cut(s) 406, 985
BsrGI TGTACA 1 cut(s) 1068
BsrI ACTGG 3 cut(s) 284, 502, 1143
BssECI CCNNGG 1 cut(s) 546
BssMI GATC 7 cut(s) 232, 382, 472, 526, 619, 669, 1165
Bst2UI CCWGG 1 cut(s) 548
Bst4CI ACNGT 7 cut(s) 247, 269, 322, 1015, 1021, 1027, 1214
Bst6I CTCTTC 1 cut(s) 933
BstAUI TGTACA 1 cut(s) 1068
BstC8I GCNNGC 1 cut(s) 1082
BstDEI CTNAG 8 cut(s) 54, 121, 213, 356, 585, 681, 702, 874
BstF5I GGATG 3 cut(s) 171, 357, 654
BstKTI GATC 7 cut(s) 235, 385, 475, 529, 622, 672, 1168
BstMAI GTCTC 4 cut(s) 30, 253, 696, 1172
BstMBI GATC 7 cut(s) 232, 382, 472, 526, 619, 669, 1165
BstMWI GCNNNNNNNGC 4 cut(s) 58, 228, 705, 896
BstNI CCWGG 1 cut(s) 548
BstNSI RCATGY 2 cut(s) 1069, 1084
BstSCI CCNGG 1 cut(s) 546
BstSFI CTRYAG 1 cut(s) 762
BstV1I GCAGC 3 cut(s) 840, 1060, 1096
BstV2I GAAGAC 2 cut(s) 280, 645
BstX2I RGATCY 3 cut(s) 526, 619, 669
BstYI RGATCY 3 cut(s) 526, 619, 669
BsuI GTATCC 1 cut(s) 97
BsuRI GGCC 2 cut(s) 82, 551
BtrI CACGTC 1 cut(s) 228
BtsCI GGATG 3 cut(s) 171, 357, 654
BtsI GCAGTG 1 cut(s) 1198
BtsIMutI CAGTG 4 cut(s) 723, 1092, 1161, 1198
Cac8I GCNNGC 1 cut(s) 1082
CaiI CAGNNNCTG 1 cut(s) 391
Cfr13I GGNCC 2 cut(s) 80, 1126
Csp6I GTAC 3 cut(s) 72, 661, 1069
CviAII CATG 7 cut(s) 591, 675, 758, 800, 1066, 1072, 1081
CviQI GTAC 3 cut(s) 72, 661, 1069
DdeI CTNAG 8 cut(s) 54, 121, 213, 356, 585, 681, 702, 874
DpnI GATC 7 cut(s) 234, 384, 474, 528, 621, 671, 1167
DpnII GATC 7 cut(s) 232, 382, 472, 526, 619, 669, 1165
Eam1104I CTCTTC 1 cut(s) 933
EarI CTCTTC 1 cut(s) 933
Eco47I GGWCC 1 cut(s) 1126
Eco88I CYCGRG 2 cut(s) 143, 1156
EcoRII CCWGG 1 cut(s) 546
FaeI CATG 7 cut(s) 594, 678, 761, 803, 1069, 1075, 1084
FaqI GGGAC 2 cut(s) 182, 493
FatI CATG 7 cut(s) 590, 674, 757, 799, 1065, 1071, 1080
FauNDI CATATG 1 cut(s) 883
FbaI TGATCA 1 cut(s) 1165
FblI GTMKAC 2 cut(s) 776, 1062
Fnu4HI GCNGC 3 cut(s) 829, 1049, 1085
FokI GGATG 3 cut(s) 158, 364, 661
Fsp4HI GCNGC 3 cut(s) 829, 1049, 1085
FspBI CTAG 2 cut(s) 165, 738
GluI GCNGC 3 cut(s) 829, 1049, 1085
GsaI CCCAGC 2 cut(s) 395, 491
GsuI CTGGAG 1 cut(s) 543
HaeIII GGCC 2 cut(s) 82, 551
Hin1II CATG 7 cut(s) 594, 678, 761, 803, 1069, 1075, 1084
HincII GTYRAC 2 cut(s) 777, 837
HindII GTYRAC 2 cut(s) 777, 837
HindIII AAGCTT 3 cut(s) 769, 809, 1115
HphI GGTGA 3 cut(s) 974, 1015, 1128
Hpy166II GTNNAC 4 cut(s) 151, 777, 837, 1063
Hpy188III TCNNGA 8 cut(s) 143, 175, 236, 367, 380, 917, 1031, 1149
Hpy8I GTNNAC 4 cut(s) 151, 777, 837, 1063
HpyCH4III ACNGT 7 cut(s) 247, 269, 322, 1015, 1021, 1027, 1214
HpyCH4IV ACGT 2 cut(s) 153, 227
HpyCH4V TGCA 5 cut(s) 186, 568, 857, 1048, 1084
HpyF10VI GCNNNNNNNGC 4 cut(s) 58, 228, 705, 896
HpyF3I CTNAG 8 cut(s) 54, 121, 213, 356, 585, 681, 702, 874
HpySE526I ACGT 2 cut(s) 153, 227
Hsp92II CATG 7 cut(s) 594, 678, 761, 803, 1069, 1075, 1084
Ksp22I TGATCA 1 cut(s) 1165
Kzo9I GATC 7 cut(s) 232, 382, 472, 526, 619, 669, 1165
LmnI GCTCC 1 cut(s) 895
Lsp1109I GCAGC 3 cut(s) 840, 1060, 1096
LweI GCATC 5 cut(s) 91, 342, 866, 877, 970
MaeI CTAG 2 cut(s) 165, 738
MaeII ACGT 2 cut(s) 153, 227
MaeIII GTNAC 4 cut(s) 116, 712, 1015, 1021
MalI GATC 7 cut(s) 234, 384, 474, 528, 621, 671, 1167
MboI GATC 7 cut(s) 232, 382, 472, 526, 619, 669, 1165
MboII GAAGA 7 cut(s) 280, 368, 456, 650, 950, 955, 1219
MflI RGATCY 3 cut(s) 526, 619, 669
MhlI GDGCHC 1 cut(s) 1163
MluCI AATT 4 cut(s) 370, 596, 970, 1088
MlyI GAGTC 5 cut(s) 566, 596, 698, 800, 887
MnlI CCTC 8 cut(s) 21, 139, 340, 344, 434, 521, 562, 639
MroXI GAANNNNTTC 2 cut(s) 62, 452
MseI TTAA 4 cut(s) 264, 429, 666, 969
MslI CAYNNNNRTG 1 cut(s) 1070
MspR9I CCNGG 1 cut(s) 548
MvaI CCWGG 1 cut(s) 548
MwoI GCNNNNNNNGC 4 cut(s) 58, 228, 705, 896
NdeI CATATG 1 cut(s) 883
NdeII GATC 7 cut(s) 232, 382, 472, 526, 619, 669, 1165
NlaIII CATG 7 cut(s) 594, 678, 761, 803, 1069, 1075, 1084
NlaIV GGNNCC 3 cut(s) 408, 987, 1198
NmeAIII GCCGAG 1 cut(s) 73
NmuCI GTSAC 3 cut(s) 712, 1015, 1021
NspI RCATGY 2 cut(s) 1069, 1084
PaeI GCATGC 1 cut(s) 1084
PaeR7I CTCGAG 2 cut(s) 143, 1156
PciI ACATGT 1 cut(s) 1065
PcsI WCGNNNNNNNCGW 1 cut(s) 150
PdmI GAANNNNTTC 2 cut(s) 62, 452
PfeI GAWTC 5 cut(s) 5, 326, 815, 1097, 1133
PflMI CCANNNNNTGG 1 cut(s) 649
PkrI GCNGC 3 cut(s) 830, 1050, 1086
PleI GAGTC 5 cut(s) 566, 595, 697, 799, 886
PpsI GAGTC 5 cut(s) 566, 595, 697, 799, 886
PscI ACATGT 1 cut(s) 1065
PshBI ATTAAT 1 cut(s) 969
Psp6I CCWGG 1 cut(s) 546
PspFI CCCAGC 2 cut(s) 391, 487
PspGI CCWGG 1 cut(s) 546
PspN4I GGNNCC 3 cut(s) 408, 987, 1198
PspPI GGNCC 2 cut(s) 80, 1126
PspXI VCTCGAGB 1 cut(s) 1156
PstNI CAGNNNCTG 1 cut(s) 391
PsuI RGATCY 3 cut(s) 526, 619, 669
RsaI GTAC 3 cut(s) 73, 662, 1070
RsaNI GTAC 3 cut(s) 72, 661, 1069
RseI CAYNNNNRTG 1 cut(s) 1070
SalI GTCGAC 1 cut(s) 775
SaqAI TTAA 4 cut(s) 264, 429, 666, 969
SatI GCNGC 3 cut(s) 829, 1049, 1085
Sau3AI GATC 7 cut(s) 232, 382, 472, 526, 619, 669, 1165
Sau96I GGNCC 2 cut(s) 80, 1126
SchI GAGTC 5 cut(s) 566, 596, 698, 800, 887
ScrFI CCNGG 1 cut(s) 548
SduI GDGCHC 1 cut(s) 1163
SfaNI GCATC 5 cut(s) 91, 342, 866, 877, 970
SfcI CTRYAG 1 cut(s) 762
Sfr274I CTCGAG 2 cut(s) 143, 1156
SinI GGWCC 1 cut(s) 1126
SlaI CTCGAG 2 cut(s) 143, 1156
SmiMI CAYNNNNRTG 1 cut(s) 1070
SmlI CTYRAG 2 cut(s) 143, 1156
SmoI CTYRAG 2 cut(s) 143, 1156
SphI GCATGC 1 cut(s) 1084
Sse9I AATT 4 cut(s) 370, 596, 970, 1088
SsiI CCGC 2 cut(s) 983, 1043
SspMI CTAG 2 cut(s) 165, 738
StyD4I CCNGG 1 cut(s) 546
TaaI ACNGT 7 cut(s) 247, 269, 322, 1015, 1021, 1027, 1214
TaiI ACGT 2 cut(s) 156, 230
TaqI TCGA 3 cut(s) 144, 776, 1157
TasI AATT 4 cut(s) 370, 596, 970, 1088
TatI WGTACW 2 cut(s) 660, 1068
TfiI GAWTC 5 cut(s) 5, 326, 815, 1097, 1133
Tru1I TTAA 4 cut(s) 264, 429, 666, 969
Tru9I TTAA 4 cut(s) 264, 429, 666, 969
TscAI CASTG 4 cut(s) 730, 1099, 1168, 1198
TseFI GTSAC 3 cut(s) 712, 1015, 1021
TseI GCWGC 3 cut(s) 828, 1048, 1084
Tsp45I GTSAC 3 cut(s) 712, 1015, 1021
TspDTI ATGAA 8 cut(s) 55, 121, 146, 470, 651, 746, 1088, 1127
TspRI CASTG 4 cut(s) 730, 1099, 1168, 1198
Van91I CCANNNNNTGG 1 cut(s) 649
VpaK11BI GGWCC 1 cut(s) 1126
VspI ATTAAT 1 cut(s) 969
XceI RCATGY 2 cut(s) 1069, 1084
XhoI CTCGAG 2 cut(s) 143, 1156
XmiI GTMKAC 2 cut(s) 776, 1062
XmnI GAANNNNTTC 2 cut(s) 62, 452
XspI CTAG 2 cut(s) 165, 738
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.