Rh5AG286500

C2 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0006102
Physical Location & Seq
Forward (+)
38769035 .. 38770300
1266 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG286500.1

Sequence Viewer

Length: 1266 bp
ATGGATTCCCCTCAATCTGTTGTGTCTCCGTTCAAAACCTCTGTTGTTTCTGAGCTTGAGAAGCAGAAATCTGACTGTTCTGTTTACACCAACTCTGGACCATGTTCTAATGGAATTGAGGCCAATAGACAGGAAACCAATGTTTGTAATGTAGAGGACTTCATTGGTGTTCTTGAGGTGTATGTTCATCAGGCTAGGGACATCCACAACATCTGCATTTACCACAAGCAAGATGTCTATGCTAAGCTGTGCCTGACAAGTGATCCAGAAATTACAGTATCCACCAAAACCATTAATGGTGGTGGTCAAAATCCAGTCTTCAATGACAAGGTCCAGCTCAATGTTCGGACTGTTGATGGCTCCGTCAAATGCGAGATTTGGATGCTGAGCAGGGTGAAGAATTATCTTGAAGATCAGTTGCTGGGGTTTGCTTTGGTGCCTATGTCAGAGGTCCTCATCAAGAATGGGAAGTTAGAGAAAGAGTTCTCTCTTTCTTCAAACGATCTCTTTCATTCCCCAGCAGGGTTTGTGAAATTGTCTCTCTCCTATGCTGGAGATTCACCAGAAGTGATGTCCTTGCCTTCAAATCCTATTATGGAGGATACAGAGATAACTGATTCTGTTCCTTGTGAAGAATTAGATAAGATAGAGTTCCCAGACCCTGATATTGCCAATGAAGACCAGCTGATGGTTTCAGAGTATATTAAGATCCCATGTTCGGAGTTTGAGTCTCAGAGCTCTGAGAGTTTTGTCACTGCTGATTGTGAAACTCATGTTAGTTCTGAAGTGGGCATTCAAGCAGTAGAAAGCGTTTCGACTGCTGCATTTGGATCTATTCAGGCTCAGAAGCTTGACTCTCCCCCTAGCAGTGTGTCAACTAATGGGGTTTCATCTCCATCTGTCCAGGCCAGCTCAGAGTCATCTGACGCTCCTGCATCTCCAAATCAGGAACAAGTTTCGGCCTTAGAAGAGAAAAAGGCAGATCGTAAAGATGGCGAGACTGATTCATCTGGTGGGGTGCCAAGTGATACAACCTCAAAACCTGTCGTCACTGTCAATGTTCCAGAGCAAAATGTGGTGCAGCAGGATTTTGTAGACATGTACATGAAAAGCATGCAGCAATTTACTGAGTCTTTGGCTAAGATGAAGCTTCCTTTGGACATTGAATCACCAACTAGTTCAGGAAATTCGAGCTCTGATCAGAATTTATCAACACCAAAGACAAATGGCTCTCGTGTGTTTTATGGGAGTAGAGCTTTCTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2

Protein Analysis

421

Amino Acids

45.72

Weight (kDa)

4.42

Isoelectric Point (pI)

57.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
C2 PF00168 56 - 159 2.2e-09 C2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 436, 1018
AccB7I CCANNNNNTGG 1 cut(s) 688
AccI GTMKAC 1 cut(s) 1095
AclWI GGATC 3 cut(s) 257, 703, 838
AcsI RAATTY 2 cut(s) 1186, 1204
AcuI CTGAAG 1 cut(s) 804
AfaI GTAC 1 cut(s) 1103
AfiI CCNNNNNNNGG 3 cut(s) 522, 688, 718
AflIII ACRYGT 1 cut(s) 1098
AgsI TTSAA 7 cut(s) 34, 322, 410, 498, 585, 797, 1166
AhlI ACTAGT 1 cut(s) 1175
AjnI CCWGG 1 cut(s) 903
AjuI GAANNNNNNNTTGG 2 cut(s) 1139, 1171
Alw21I GWGCWC 2 cut(s) 740, 1196
Alw26I GTCTC 4 cut(s) 30, 543, 735, 992
AlwI GGATC 3 cut(s) 257, 703, 838
AlwNI CAGNNNCTG 2 cut(s) 421, 662
AoxI GGCC 3 cut(s) 120, 906, 960
ApeKI GCWGC 3 cut(s) 821, 1081, 1117
ApoI RAATTY 2 cut(s) 1186, 1204
AseI ATTAAT 1 cut(s) 294
Asp700I GAANNNNTTC 1 cut(s) 482
AspS9I GGNCC 3 cut(s) 98, 331, 451
AsuHPI GGTGA 3 cut(s) 406, 552, 1161
AvaII GGWCC 3 cut(s) 98, 331, 451
BanI GGYRCC 2 cut(s) 436, 1018
BanII GRGCYC 2 cut(s) 740, 1196
BauI CACGAG 1 cut(s) 1233
BbsI GAAGAC 2 cut(s) 310, 684
Bbv12I GWGCWC 2 cut(s) 740, 1196
BbvI GCAGC 3 cut(s) 808, 1093, 1129
BccI CCATC 4 cut(s) 350, 682, 904, 986
BciT130I CCWGG 1 cut(s) 905
BciVI GTATCC 2 cut(s) 289, 595
BclI TGATCA 1 cut(s) 1198
BcoDI GTCTC 4 cut(s) 30, 543, 735, 992
BcuI ACTAGT 1 cut(s) 1175
BfaI CTAG 3 cut(s) 195, 864, 1176
BfuI GTATCC 2 cut(s) 289, 595
BisI GCNGC 3 cut(s) 822, 1082, 1118
BlpI GCTNAGC 2 cut(s) 243, 386
BlsI GCNGC 3 cut(s) 823, 1083, 1119
Bme1390I CCNGG 1 cut(s) 905
Bme18I GGWCC 3 cut(s) 98, 331, 451
BmgT120I GGNCC 3 cut(s) 98, 331, 451
BmiI GGNNCC 3 cut(s) 361, 438, 1020
BmrFI CCNGG 1 cut(s) 905
BmsI GCATC 2 cut(s) 372, 944
BpiI GAAGAC 2 cut(s) 310, 684
BpmI CTGGAG 1 cut(s) 573
Bpu1102I GCTNAGC 2 cut(s) 243, 386
BpuEI CTTGAG 2 cut(s) 77, 194
BsaXI ACNNNNNCTCC 2 cut(s) 713, 743
Bsc4I CCNNNNNNNGG 3 cut(s) 522, 688, 718
Bse1I ACTGG 1 cut(s) 314
BseBI CCWGG 1 cut(s) 905
BseGI GGATG 2 cut(s) 201, 387
BseLI CCNNNNNNNGG 3 cut(s) 522, 688, 718
BseMII CTCAG 7 cut(s) 42, 377, 732, 746, 857, 927, 1119
BseNI ACTGG 1 cut(s) 314
BseXI GCAGC 3 cut(s) 808, 1093, 1129
BseYI CCCAGC 2 cut(s) 421, 517
BsgI GTGCAG 1 cut(s) 1100
BshFI GGCC 3 cut(s) 122, 908, 962
BshNI GGYRCC 2 cut(s) 436, 1018
BsiHKAI GWGCWC 2 cut(s) 740, 1196
BslFI GGGAC 1 cut(s) 212
BslI CCNNNNNNNGG 3 cut(s) 522, 688, 718
BsmAI GTCTC 4 cut(s) 30, 543, 735, 992
BsmFI GGGAC 1 cut(s) 212
BsmI GAATGC 1 cut(s) 792
BsnI GGCC 3 cut(s) 122, 908, 962
Bsp1286I GDGCHC 2 cut(s) 740, 1196
Bsp1407I TGTACA 1 cut(s) 1101
Bsp143I GATC 7 cut(s) 262, 412, 502, 708, 830, 982, 1198
Bsp1720I GCTNAGC 2 cut(s) 243, 386
BspANI GGCC 3 cut(s) 122, 908, 962
BspCNI CTCAG 7 cut(s) 43, 378, 733, 745, 856, 926, 1120
BspLI GGNNCC 3 cut(s) 361, 438, 1020
BspPI GGATC 3 cut(s) 257, 703, 838
BspT107I GGYRCC 2 cut(s) 436, 1018
BsrGI TGTACA 1 cut(s) 1101
BsrI ACTGG 1 cut(s) 314
BssMI GATC 7 cut(s) 262, 412, 502, 708, 830, 982, 1198
BssSI CACGAG 1 cut(s) 1233
Bst2BI CACGAG 1 cut(s) 1233
Bst2UI CCWGG 1 cut(s) 905
Bst4CI ACNGT 4 cut(s) 77, 277, 352, 1054
Bst6I CTCTTC 1 cut(s) 963
BstAUI TGTACA 1 cut(s) 1101
BstC8I GCNNGC 2 cut(s) 910, 1115
BstF5I GGATG 2 cut(s) 201, 387
BstKTI GATC 7 cut(s) 265, 415, 505, 711, 833, 985, 1201
BstMAI GTCTC 4 cut(s) 30, 543, 735, 992
BstMBI GATC 7 cut(s) 262, 412, 502, 708, 830, 982, 1198
BstMWI GCNNNNNNNGC 1 cut(s) 61
BstNI CCWGG 1 cut(s) 905
BstNSI RCATGY 2 cut(s) 1102, 1117
BstSCI CCNGG 1 cut(s) 903
BstV1I GCAGC 3 cut(s) 808, 1093, 1129
BstV2I GAAGAC 2 cut(s) 310, 684
BstX2I RGATCY 2 cut(s) 708, 830
BstYI RGATCY 2 cut(s) 708, 830
BsuI GTATCC 2 cut(s) 289, 595
BsuRI GGCC 3 cut(s) 122, 908, 962
BtsCI GGATG 2 cut(s) 201, 387
BtsI GCAGTG 2 cut(s) 753, 874
BtsIMutI CAGTG 3 cut(s) 753, 874, 1050
Cac8I GCNNGC 2 cut(s) 910, 1115
CaiI CAGNNNCTG 2 cut(s) 421, 662
Cfr13I GGNCC 3 cut(s) 98, 331, 451
CseI GACGC 1 cut(s) 935
Csp6I GTAC 1 cut(s) 1102
CviAII CATG 6 cut(s) 102, 714, 773, 1099, 1105, 1114
CviQI GTAC 1 cut(s) 1102
DpnI GATC 7 cut(s) 264, 414, 504, 710, 832, 984, 1200
DpnII GATC 7 cut(s) 262, 412, 502, 708, 830, 982, 1198
Eam1104I CTCTTC 1 cut(s) 963
EarI CTCTTC 1 cut(s) 963
Ecl136II GAGCTC 2 cut(s) 738, 1194
Eco24I GRGCYC 2 cut(s) 740, 1196
Eco47I GGWCC 3 cut(s) 98, 331, 451
Eco53kI GAGCTC 2 cut(s) 738, 1194
Eco57I CTGAAG 1 cut(s) 804
EcoICRI GAGCTC 2 cut(s) 738, 1194
EcoO109I RGGNCCY 1 cut(s) 451
EcoRII CCWGG 1 cut(s) 903
EcoT38I GRGCYC 2 cut(s) 740, 1196
FaeI CATG 6 cut(s) 105, 717, 776, 1102, 1108, 1117
FaqI GGGAC 1 cut(s) 212
FatI CATG 6 cut(s) 101, 713, 772, 1098, 1104, 1113
FbaI TGATCA 1 cut(s) 1198
FblI GTMKAC 1 cut(s) 1095
Fnu4HI GCNGC 3 cut(s) 822, 1082, 1118
FokI GGATG 2 cut(s) 188, 394
FriOI GRGCYC 2 cut(s) 740, 1196
Fsp4HI GCNGC 3 cut(s) 822, 1082, 1118
FspBI CTAG 3 cut(s) 195, 864, 1176
GluI GCNGC 3 cut(s) 822, 1082, 1118
GsaI CCCAGC 2 cut(s) 425, 521
GsuI CTGGAG 1 cut(s) 573
HaeIII GGCC 3 cut(s) 122, 908, 962
HgaI GACGC 1 cut(s) 935
Hin1II CATG 6 cut(s) 105, 717, 776, 1102, 1108, 1117
HincII GTYRAC 1 cut(s) 876
HindII GTYRAC 1 cut(s) 876
HindIII AAGCTT 2 cut(s) 848, 1148
HinfI GANTC 9 cut(s) 5, 557, 617, 728, 854, 917, 1004, 1130, 1166
HphI GGTGA 3 cut(s) 406, 552, 1161
Hpy166II GTNNAC 3 cut(s) 85, 876, 1096
Hpy188III TCNNGA 8 cut(s) 96, 173, 266, 407, 460, 947, 1064, 1182
Hpy8I GTNNAC 3 cut(s) 85, 876, 1096
HpyAV CCTTC 1 cut(s) 591
HpyCH4III ACNGT 4 cut(s) 77, 277, 352, 1054
HpyCH4V TGCA 5 cut(s) 216, 824, 935, 1081, 1117
HpyF10VI GCNNNNNNNGC 1 cut(s) 61
Hsp92II CATG 6 cut(s) 105, 717, 776, 1102, 1108, 1117
Ksp22I TGATCA 1 cut(s) 1198
Kzo9I GATC 7 cut(s) 262, 412, 502, 708, 830, 982, 1198
LmnI GCTCC 2 cut(s) 365, 934
Lsp1109I GCAGC 3 cut(s) 808, 1093, 1129
LweI GCATC 2 cut(s) 372, 944
MaeI CTAG 3 cut(s) 195, 864, 1176
MaeIII GTNAC 2 cut(s) 751, 1048
MalI GATC 7 cut(s) 264, 414, 504, 710, 832, 984, 1200
MboI GATC 7 cut(s) 262, 412, 502, 708, 830, 982, 1198
MboII GAAGA 8 cut(s) 310, 409, 422, 486, 644, 689, 980, 1252
MflI RGATCY 2 cut(s) 708, 830
MhlI GDGCHC 2 cut(s) 740, 1196
MluCI AATT 8 cut(s) 114, 270, 400, 533, 635, 1121, 1186, 1204
MlyI GAGTC 4 cut(s) 737, 848, 926, 1139
MnlI CCTC 9 cut(s) 21, 49, 112, 148, 169, 442, 464, 592, 1045
MroXI GAANNNNTTC 1 cut(s) 482
MseI TTAA 2 cut(s) 294, 705
MslI CAYNNNNRTG 1 cut(s) 1103
MspA1I CMGCKG 1 cut(s) 685
MspR9I CCNGG 1 cut(s) 905
Mva1269I GAATGC 1 cut(s) 792
MvaI CCWGG 1 cut(s) 905
MwoI GCNNNNNNNGC 1 cut(s) 61
NdeII GATC 7 cut(s) 262, 412, 502, 708, 830, 982, 1198
NlaIII CATG 6 cut(s) 105, 717, 776, 1102, 1108, 1117
NlaIV GGNNCC 3 cut(s) 361, 438, 1020
NmuCI GTSAC 2 cut(s) 751, 1048
NspI RCATGY 2 cut(s) 1102, 1117
PaeI GCATGC 1 cut(s) 1117
PciI ACATGT 1 cut(s) 1098
PctI GAATGC 1 cut(s) 792
PdmI GAANNNNTTC 1 cut(s) 482
PfeI GAWTC 5 cut(s) 5, 557, 617, 1004, 1166
PflFI GACNNNGTC 1 cut(s) 329
PflMI CCANNNNNTGG 1 cut(s) 688
PkrI GCNGC 3 cut(s) 823, 1083, 1119
PleI GAGTC 4 cut(s) 736, 848, 925, 1138
PpsI GAGTC 4 cut(s) 736, 848, 925, 1138
PpuMI RGGWCCY 1 cut(s) 451
PscI ACATGT 1 cut(s) 1098
PshBI ATTAAT 1 cut(s) 294
Psp124BI GAGCTC 2 cut(s) 740, 1196
Psp5II RGGWCCY 1 cut(s) 451
Psp6I CCWGG 1 cut(s) 903
PspFI CCCAGC 2 cut(s) 421, 517
PspGI CCWGG 1 cut(s) 903
PspN4I GGNNCC 3 cut(s) 361, 438, 1020
PspPI GGNCC 3 cut(s) 98, 331, 451
PspPPI RGGWCCY 1 cut(s) 451
PstNI CAGNNNCTG 2 cut(s) 421, 662
PsuI RGATCY 2 cut(s) 708, 830
PsyI GACNNNGTC 1 cut(s) 329
PvuII CAGCTG 1 cut(s) 685
RsaI GTAC 1 cut(s) 1103
RsaNI GTAC 1 cut(s) 1102
RseI CAYNNNNRTG 1 cut(s) 1103
SacI GAGCTC 2 cut(s) 740, 1196
SaqAI TTAA 2 cut(s) 294, 705
SatI GCNGC 3 cut(s) 822, 1082, 1118
Sau3AI GATC 7 cut(s) 262, 412, 502, 708, 830, 982, 1198
Sau96I GGNCC 3 cut(s) 98, 331, 451
SchI GAGTC 4 cut(s) 737, 848, 926, 1139
ScrFI CCNGG 1 cut(s) 905
SduI GDGCHC 2 cut(s) 740, 1196
SfaNI GCATC 2 cut(s) 372, 944
SinI GGWCC 3 cut(s) 98, 331, 451
SmiMI CAYNNNNRTG 1 cut(s) 1103
SmlI CTYRAG 2 cut(s) 56, 173
SmoI CTYRAG 2 cut(s) 56, 173
SpeI ACTAGT 1 cut(s) 1175
SphI GCATGC 1 cut(s) 1117
Sse9I AATT 8 cut(s) 114, 270, 400, 533, 635, 1121, 1186, 1204
SspMI CTAG 3 cut(s) 195, 864, 1176
SstI GAGCTC 2 cut(s) 740, 1196
StyD4I CCNGG 1 cut(s) 903
TaaI ACNGT 4 cut(s) 77, 277, 352, 1054
TaqI TCGA 2 cut(s) 815, 1190
TasI AATT 8 cut(s) 114, 270, 400, 533, 635, 1121, 1186, 1204
TatI WGTACW 1 cut(s) 1101
TfiI GAWTC 5 cut(s) 5, 557, 617, 1004, 1166
Tru1I TTAA 2 cut(s) 294, 705
Tru9I TTAA 2 cut(s) 294, 705
TscAI CASTG 3 cut(s) 760, 874, 1057
TseFI GTSAC 2 cut(s) 751, 1048
TseI GCWGC 3 cut(s) 821, 1081, 1117
Tsp45I GTSAC 2 cut(s) 751, 1048
TspDTI ATGAA 8 cut(s) 151, 176, 500, 690, 879, 996, 1121, 1160
TspGWI ACGGA 2 cut(s) 18, 352
TspRI CASTG 3 cut(s) 760, 874, 1057
Tth111I GACNNNGTC 1 cut(s) 329
Van91I CCANNNNNTGG 1 cut(s) 688
VpaK11BI GGWCC 3 cut(s) 98, 331, 451
VspI ATTAAT 1 cut(s) 294
XapI RAATTY 2 cut(s) 1186, 1204
XceI RCATGY 2 cut(s) 1102, 1117
XmiI GTMKAC 1 cut(s) 1095
XmnI GAANNNNTTC 1 cut(s) 482
XspI CTAG 3 cut(s) 195, 864, 1176
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.