AT5G55590

Pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0013350
Physical Location & Seq
Forward (+)
22519654 .. 22521858
2205 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G55590.2

Sequence Viewer

Length: 1143 bp
ATGAAAGTCGAAGCTTTTATTCCCGCCGTTTTATTGCTCTGTTTTGGGGTAATGTTATGTTTAAAAAGCTCGTGTGCGTTGCAGATAGGTAACAATAATGAGTTGAAGAATTACATAAGTTGGGAAGATTTGAGAGTTGTGGAAGATGGAAGAATAGAGAGAAGTTTTAGCATTAAGGAGAATAGTAATTGGGTAACCACAAACGCTAACGCTAACGCGAACGCTACTAACGTGAGAAGAGTGATTGTGGTTGATAAAAACGGAGGAGGAGATTCTGTTACAGTTCAAGGAGCTGTTGATATGGTTCCTGATTCCAATTCACAGAGAGTTAAAATCTTCATTCTTCCTGGGATTTACAGGGAGAAGGTGATTGTGCCGAAATCAAAACCGTATATTTCGTTCATAGGTAATGAGAGCTATGCGGGAGATACAGTGATTAGTTGGAGCGATAAAGCTTCAGACCTTGGCTGCGATGGTAAAGAACTCGGCACTTATAGAACCGCCTCTGTTTCCATTGAATCTGATTTCTTCTGTGCTACTGCCATCACTTTTGAGAACACGGTGGTTGCAGAGGCAGGGGAACAGGGGAGGCAAGCGGTGGCGTTGAGAATAATTGGGGACAAAGCTGTGTTCTATAGAGTTAGGGTTTTGGGATCACAAGACACTCTTTTTGATGACAATGGATCTCACTACTTTTACCAATGCTATATCCAAGGCAATGTAGATTTCATCTTTGGCAATGCGAAATCACTTTACCAGGACTGTGATATCCACTCAACCGCAAAGAGATATGGCGCGATCGCGGCTCATCATAGAGACTCGGAGACTGAAGATACCGGGTTCTCCTTTGTGAACTGTGATATCAGTGGTACTGGGCAGATTTACTTAGGAAGGGCTTGGGGAAACTACTCAAGAACTGTTTATTCAAACTGTTTCATAGCTGATATTATCACTCCTGTGGGCTGGAGTGACTGGAAACACCCTGAGAGGCAAAGGAAAGTGATGTTCGGGGAGTACAATTGCAGGGGAAGAGGAGCAGAGAGAGGAGGCCGAGTACCGTGGTCGAAAACTCTTACCAGAGATGAAGTGAAGCCTTTTCTGGGAAGAGAGTTCATATATGGAGATCAATGGCTGAGACTCTAA

Protein Analysis

380

Amino Acids

42.47

Weight (kDa)

6.26

Isoelectric Point (pI)

24.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 82 - 327 4.4e-58 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0013350)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G55590 AT5G55590
fragaria_vesca FvH4_3g29490
malus_domestica MD03G1186900.v1.1 MD11G1201900.v1.1
prunus_persica Prupe.4G239700_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0054371
rosa_laevigata RLG00000034927
rosa_multiflora Rmu_sc0005705.1_g000023 Rmu_sc0023043.1_g000001
rosa_roxburghii Rroxscaffold_1G00026500
rosa_rugosa Rorug05G0285900
rosa_samantha Rh5AG356400 Rh5BG368000 Rh5CG391300 Rh5DG382200
rosa_wichuraiana Rw5G033480 Rw5G033650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 218, 797, 803
AciI CCGC 6 cut(s) 24, 422, 501, 596, 780, 803
AclWI GGATC 2 cut(s) 661, 691
AcuI CTGAAG 2 cut(s) 441, 849
AfaI GTAC 3 cut(s) 871, 1016, 1056
AfiI CCNNNNNNNGG 1 cut(s) 1100
AgsI TTSAA 4 cut(s) 106, 287, 518, 927
AjnI CCWGG 2 cut(s) 346, 756
AleI CACNNNNGTG 1 cut(s) 956
AluBI AGCT 7 cut(s) 14, 69, 293, 417, 455, 626, 941
AluI AGCT 7 cut(s) 14, 69, 293, 417, 455, 626, 941
Alw26I GTCTC 3 cut(s) 810, 818, 1129
AlwI GGATC 2 cut(s) 661, 691
AoxI GGCC 1 cut(s) 1048
ApeKI GCWGC 1 cut(s) 468
ArsI GACNNNNNNTTYG 2 cut(s) 653, 685
AsiSI GCGATCGC 1 cut(s) 801
AspLEI GCGC 1 cut(s) 797
AsuC2I CCSGG 1 cut(s) 838
AsuHPI GGTGA 1 cut(s) 379
BauI CACGAG 1 cut(s) 70
BbvI GCAGC 1 cut(s) 455
BccI CCATC 3 cut(s) 140, 467, 551
BceAI ACGGC 1 cut(s) 11
BciT130I CCWGG 2 cut(s) 348, 758
BcnI CCSGG 1 cut(s) 838
BcoDI GTCTC 3 cut(s) 810, 818, 1129
BfmI CTRYAG 1 cut(s) 634
BisI GCNGC 2 cut(s) 469, 804
BlsI GCNGC 2 cut(s) 470, 805
Bme1390I CCNGG 3 cut(s) 348, 758, 838
BmiI GGNNCC 1 cut(s) 306
BmrFI CCNGG 3 cut(s) 348, 758, 838
BmrI ACTGGG 1 cut(s) 882
BmuI ACTGGG 1 cut(s) 882
BpmI CTGGAG 1 cut(s) 985
BpuEI CTTGAG 1 cut(s) 895
BpuMI CCSGG 1 cut(s) 838
BsaJI CCNNGG 4 cut(s) 347, 463, 712, 1058
BsaXI ACNNNNNCTCC 6 cut(s) 261, 291, 417, 447, 1038, 1068
Bsc4I CCNNNNNNNGG 1 cut(s) 1100
Bse1I ACTGG 2 cut(s) 877, 977
Bse3DI GCAATG 2 cut(s) 724, 745
BseBI CCWGG 2 cut(s) 348, 758
BseDI CCNNGG 4 cut(s) 347, 463, 712, 1058
BseLI CCNNNNNNNGG 1 cut(s) 1100
BseMI GCAATG 2 cut(s) 724, 745
BseMII CTCAG 2 cut(s) 975, 1124
BseNI ACTGG 2 cut(s) 877, 977
BseRI GAGGAG 4 cut(s) 279, 282, 1047, 1059
BseXI GCAGC 1 cut(s) 455
Bsh1236I CGCG 3 cut(s) 218, 797, 803
Bsh1285I CGRYCG 1 cut(s) 801
BshFI GGCC 1 cut(s) 1050
BsiEI CGRYCG 1 cut(s) 801
BsiSI CCGG 1 cut(s) 837
BslFI GGGAC 1 cut(s) 632
BslI CCNNNNNNNGG 1 cut(s) 1100
BsmAI GTCTC 3 cut(s) 810, 818, 1129
BsmFI GGGAC 1 cut(s) 632
BsnI GGCC 1 cut(s) 1050
Bsp143I GATC 4 cut(s) 653, 683, 798, 1123
BspACI CCGC 6 cut(s) 24, 422, 501, 596, 780, 803
BspANI GGCC 1 cut(s) 1050
BspCNI CTCAG 2 cut(s) 976, 1125
BspFNI CGCG 3 cut(s) 218, 797, 803
BspLI GGNNCC 1 cut(s) 306
BspPI GGATC 2 cut(s) 661, 691
BsrDI GCAATG 2 cut(s) 724, 745
BsrI ACTGG 2 cut(s) 877, 977
BssECI CCNNGG 4 cut(s) 347, 463, 712, 1058
BssMI GATC 4 cut(s) 653, 683, 798, 1123
BssSI CACGAG 1 cut(s) 70
BssT1I CCWWGG 2 cut(s) 463, 712
Bst2BI CACGAG 1 cut(s) 70
Bst2UI CCWGG 2 cut(s) 348, 758
Bst4CI ACNGT 9 cut(s) 283, 390, 433, 562, 764, 857, 919, 932, 1059
Bst6I CTCTTC 3 cut(s) 232, 1024, 1099
BstC8I GCNNGC 1 cut(s) 594
BstDEI CTNAG 3 cut(s) 886, 984, 1133
BstDSI CCRYGG 1 cut(s) 1058
BstEII GGTNACC 1 cut(s) 193
BstFNI CGCG 3 cut(s) 218, 797, 803
BstHHI GCGC 1 cut(s) 797
BstKTI GATC 4 cut(s) 656, 686, 801, 1126
BstMAI GTCTC 3 cut(s) 810, 818, 1129
BstMBI GATC 4 cut(s) 653, 683, 798, 1123
BstMCI CGRYCG 1 cut(s) 801
BstMWI GCNNNNNNNGC 1 cut(s) 803
BstNI CCWGG 2 cut(s) 348, 758
BstPI GGTNACC 1 cut(s) 193
BstSCI CCNGG 3 cut(s) 346, 756, 836
BstSFI CTRYAG 1 cut(s) 634
BstUI CGCG 3 cut(s) 218, 797, 803
BstV1I GCAGC 1 cut(s) 455
BstX2I RGATCY 1 cut(s) 683
BstYI RGATCY 1 cut(s) 683
BsuRI GGCC 1 cut(s) 1050
BtgI CCRYGG 1 cut(s) 1058
BtgZI GCGATG 1 cut(s) 486
BtsIMutI CAGTG 2 cut(s) 438, 871
Cac8I GCNNGC 1 cut(s) 594
CfoI GCGC 1 cut(s) 797
Csp6I GTAC 3 cut(s) 870, 1015, 1055
CviQI GTAC 3 cut(s) 870, 1015, 1055
DdeI CTNAG 3 cut(s) 886, 984, 1133
DpnI GATC 4 cut(s) 655, 685, 800, 1125
DpnII GATC 4 cut(s) 653, 683, 798, 1123
DraI TTTAAA 1 cut(s) 63
Eam1104I CTCTTC 3 cut(s) 232, 1024, 1099
EarI CTCTTC 3 cut(s) 232, 1024, 1099
Eco130I CCWWGG 2 cut(s) 463, 712
Eco32I GATATC 2 cut(s) 769, 862
Eco57I CTGAAG 2 cut(s) 441, 849
Eco91I GGTNACC 1 cut(s) 193
EcoO65I GGTNACC 1 cut(s) 193
EcoRII CCWGG 2 cut(s) 346, 756
EcoRV GATATC 2 cut(s) 769, 862
EcoT14I CCWWGG 2 cut(s) 463, 712
ErhI CCWWGG 2 cut(s) 463, 712
FalI AAGNNNNNCTT 2 cut(s) 651, 683
FaqI GGGAC 1 cut(s) 632
FauI CCCGC 2 cut(s) 31, 415
Fnu4HI GCNGC 2 cut(s) 469, 804
Fsp4HI GCNGC 2 cut(s) 469, 804
GlaI GCGC 1 cut(s) 796
GluI GCNGC 2 cut(s) 469, 804
GsuI CTGGAG 1 cut(s) 985
HaeIII GGCC 1 cut(s) 1050
HapII CCGG 1 cut(s) 837
HhaI GCGC 1 cut(s) 797
Hin6I GCGC 1 cut(s) 795
HinP1I GCGC 1 cut(s) 795
HindIII AAGCTT 2 cut(s) 12, 453
HinfI GANTC 5 cut(s) 272, 311, 518, 818, 1137
HpaII CCGG 1 cut(s) 837
HphI GGTGA 1 cut(s) 379
Hpy166II GTNNAC 1 cut(s) 853
Hpy188I TCNGA 3 cut(s) 460, 523, 823
Hpy188III TCNNGA 2 cut(s) 308, 912
Hpy8I GTNNAC 1 cut(s) 853
HpyAV CCTTC 2 cut(s) 358, 885
HpyCH4III ACNGT 9 cut(s) 283, 390, 433, 562, 764, 857, 919, 932, 1059
HpyCH4IV ACGT 1 cut(s) 231
HpyCH4V TGCA 3 cut(s) 82, 569, 1023
HpyF10VI GCNNNNNNNGC 1 cut(s) 803
HpyF3I CTNAG 3 cut(s) 886, 984, 1133
HpySE526I ACGT 1 cut(s) 231
HspAI GCGC 1 cut(s) 795
Kzo9I GATC 4 cut(s) 653, 683, 798, 1123
LmnI GCTCC 3 cut(s) 290, 444, 1034
Lsp1109I GCAGC 1 cut(s) 455
MaeII ACGT 1 cut(s) 231
MaeIII GTNAC 4 cut(s) 89, 193, 277, 968
MalI GATC 4 cut(s) 655, 685, 800, 1125
MboI GATC 4 cut(s) 653, 683, 798, 1123
MfeI CAATTG 1 cut(s) 1018
MflI RGATCY 1 cut(s) 683
MluCI AATT 5 cut(s) 109, 187, 316, 612, 1018
MlyI GAGTC 2 cut(s) 812, 1131
MmeI TCCRAC 1 cut(s) 422
MnlI CCTC 9 cut(s) 257, 260, 514, 565, 582, 981, 1025, 1037, 1040
MseI TTAA 3 cut(s) 62, 174, 330
MslI CAYNNNNRTG 1 cut(s) 956
MspI CCGG 1 cut(s) 837
MspR9I CCNGG 3 cut(s) 348, 758, 838
MunI CAATTG 1 cut(s) 1018
MvaI CCWGG 2 cut(s) 348, 758
MvnI CGCG 3 cut(s) 218, 797, 803
MwoI GCNNNNNNNGC 1 cut(s) 803
NciI CCSGG 1 cut(s) 838
NdeII GATC 4 cut(s) 653, 683, 798, 1123
NlaIV GGNNCC 1 cut(s) 306
NmeAIII GCCGAG 2 cut(s) 465, 1076
NmuCI GTSAC 1 cut(s) 968
OliI CACNNNNGTG 1 cut(s) 956
PcsI WCGNNNNNNNCGW 1 cut(s) 228
PfeI GAWTC 3 cut(s) 272, 311, 518
PkrI GCNGC 2 cut(s) 470, 805
Ple19I CGATCG 1 cut(s) 801
PleI GAGTC 2 cut(s) 812, 1131
PpsI GAGTC 2 cut(s) 812, 1131
Psp6I CCWGG 2 cut(s) 346, 756
PspEI GGTNACC 1 cut(s) 193
PspGI CCWGG 2 cut(s) 346, 756
PspN4I GGNNCC 1 cut(s) 306
PsrI GAACNNNNNNTAC 2 cut(s) 383, 415
PsuI RGATCY 1 cut(s) 683
PvuI CGATCG 1 cut(s) 801
RgaI GCGATCGC 1 cut(s) 801
RsaI GTAC 3 cut(s) 871, 1016, 1056
RsaNI GTAC 3 cut(s) 870, 1015, 1055
RseI CAYNNNNRTG 1 cut(s) 956
SaqAI TTAA 3 cut(s) 62, 174, 330
SatI GCNGC 2 cut(s) 469, 804
Sau3AI GATC 4 cut(s) 653, 683, 798, 1123
SchI GAGTC 2 cut(s) 812, 1131
ScrFI CCNGG 3 cut(s) 348, 758, 838
SfaAI GCGATCGC 1 cut(s) 801
SfcI CTRYAG 1 cut(s) 634
SgfI GCGATCGC 1 cut(s) 801
SmiMI CAYNNNNRTG 1 cut(s) 956
SmlI CTYRAG 1 cut(s) 910
SmoI CTYRAG 1 cut(s) 910
Sse9I AATT 5 cut(s) 109, 187, 316, 612, 1018
SsiI CCGC 6 cut(s) 24, 422, 501, 596, 780, 803
StyD4I CCNGG 3 cut(s) 346, 756, 836
StyI CCWWGG 2 cut(s) 463, 712
TaaI ACNGT 9 cut(s) 283, 390, 433, 562, 764, 857, 919, 932, 1059
TaiI ACGT 1 cut(s) 234
TaqI TCGA 2 cut(s) 9, 1064
TasI AATT 5 cut(s) 109, 187, 316, 612, 1018
TatI WGTACW 1 cut(s) 1014
TauI GCSGC 1 cut(s) 806
TfiI GAWTC 3 cut(s) 272, 311, 518
Tru1I TTAA 3 cut(s) 62, 174, 330
Tru9I TTAA 3 cut(s) 62, 174, 330
TscAI CASTG 2 cut(s) 438, 871
TseFI GTSAC 1 cut(s) 968
TseI GCWGC 1 cut(s) 468
Tsp45I GTSAC 1 cut(s) 968
TspDTI ATGAA 7 cut(s) 17, 328, 391, 718, 925, 1098, 1102
TspGWI ACGGA 1 cut(s) 276
TspRI CASTG 2 cut(s) 438, 871
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.