Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0013350

Physical Location & Seq

Genomic Coordinates Reverse (-)

25532395 .. 25534646

Gene Structure 2252 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1186900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1083 bp

ATGGGATTCAGTGTCTTGGGTGTTTGTGTTGGTGGTTTTGTGTTATTTTTCTTGGGTGGCATTAAGGTCGGGTTCTCGCAAAGCGAATCATTTGCAAGGAATTATATCAGCTGGGATGATTTGAAGGTGGATGACCGTAAGTTGGGGTTGAACACGAAAGAAGAACGCGTTAATGGAAGCCGGATCATTGTGGTTGACAAGAATGGAGGAGGAGATTCTCTTACAGTTCAAGGAGCAGTTGACATGGTTCCGGAACAAAATACAGAAAGAATCAAAATTTACATTCTTCCTGGAATTTACAGAGAGAAGGTACTTGTACCGATTTCGAAGCCATACATATCATTTATTGGGAACCAGAAAAATACTTCTGAAACTGTGATTACTTGGAACAACAAGGCATCTGATAAGGACGGCACCGGTTGCGAACTCGGAACATATAGAACTGCTTCTGTAGCCATAGAGGCTGATTACTTTTGCGCAACTGGGATCACCTTCGAGAACACAGTGGTTGCAGTGCCTGGAGGATATGGAATGCAAGCAGTGGCGCTTAGAGTTGCGGGTGACAAAGCCATGTTCTACAGAGTTCGGGTTTTGGGGACGCAGGATACGCTCTTGGACGAGACTGGATCTCACTACTTCTACCACTGTCACATTCAAGGAAATGTGGACTTCATTTTCGGCAGATCAAGATCACTCTACCAGTGTGTTATTCAATCAACGGCTGAGAACTCGGGAGCAATAGCAGCTCATCACAGGGACTCACCGTATGAAGACACGGGATTCTCTTTTGTAAACTGCAAAATCATCGGAACCGGCTACATCTACTTGGGAAGAGCTTGGGGAAATTATTCAAGAGCGGTGTATTCCTACTGTCATTTCGATGATATAATAACTCCTCCAGGGTGGAGCGACTGGAACTACCCATCCAGGCAGAAGACAGTGGATTTCGGAGAATATAAATGCAGGGGCAGAGGAGCAGAAAGAAGCGGGAGGGTGCCATGGTTGAAGTCTTTCAGCTTGGAGGAGATAAGGCCTTTTCTGGATACAAAATTTATATCTGGAGAGCAATGGCTTAGATTATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000272 GO:0003674 GO:0003824 GO:0005975 GO:0005976 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009987 GO:0010393 GO:0016052 GO:0016787 GO:0016788 GO:0017144 GO:0030599 GO:0042737 GO:0043170 GO:0044237 GO:0044238 GO:0044248 GO:0045488 GO:0045490 GO:0052689 GO:0071704 GO:1901575

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

Pectinesterase

Protein Analysis

361

Amino Acids

40.21

Weight (kDa)

6.99

Isoelectric Point (pI)

27.91

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pectinesterase	PF01095	63 - 354	3.2e-63	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013350)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G55590 AT5G55590
fragaria_vesca	FvH4_3g29490
malus_domestica	MD03G1186900.v1.1 MD11G1201900.v1.1
prunus_persica	Prupe.4G239700_v2.0.a1
rosa_chinensis	RchiOBHm_Chr5g0054371
rosa_laevigata	RLG00000034927
rosa_multiflora	Rmu_sc0005705.1_g000023 Rmu_sc0023043.1_g000001
rosa_roxburghii	Rroxscaffold_1G00026500
rosa_rugosa	Rorug05G0285900
rosa_samantha	Rh5AG356400 Rh5BG368000 Rh5CG391300 Rh5DG382200
rosa_wichuraiana	Rw5G033480 Rw5G033650

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1081
Acc16I	TGCGCA	1 cut(s)	478
AccB1I	GGYRCC	2 cut(s)	413, 994
AccBSI	CCGCTC	1 cut(s)	857
AccII	CGCG	1 cut(s)	168
AccIII	TCCGGA	1 cut(s)	250
AciI	CCGC	3 cut(s)	557, 857, 987
AclWI	GGATC	3 cut(s)	191, 494, 634
AcsI	RAATTY	3 cut(s)	276, 294, 1049
AfaI	GTAC	2 cut(s)	312, 318
AfiI	CCNNNNNNNGG	1 cut(s)	142
AflIII	ACRYGT	1 cut(s)	166
AgeI	ACCGGT	1 cut(s)	416
AgsI	TTSAA	7 cut(s)	124, 151, 230, 656, 713, 852, 1006
AjnI	CCWGG	4 cut(s)	289, 517, 898, 926
AloI	GAACNNNNNNTCC	2 cut(s)	908, 940
AluBI	AGCT	4 cut(s)	111, 746, 836, 1017
AluI	AGCT	4 cut(s)	111, 746, 836, 1017
Alw26I	GTCTC	1 cut(s)	614
AlwI	GGATC	3 cut(s)	191, 494, 634
AlwNI	CAGNNNCTG	1 cut(s)	518
Ama87I	CYCGRG	1 cut(s)	730
Aor13HI	TCCGGA	1 cut(s)	250
AoxI	GGCC	1 cut(s)	1031
ApeKI	GCWGC	1 cut(s)	743
ApoI	RAATTY	3 cut(s)	276, 294, 1049
ArsI	GACNNNNNNTTYG	2 cut(s)	659, 691
AsiGI	ACCGGT	1 cut(s)	416
Asp700I	GAANNNNTTC	3 cut(s)	445, 847, 1010
AspLEI	GCGC	2 cut(s)	479, 547
AsuHPI	GGTGA	3 cut(s)	481, 572, 753
AsuII	TTCGAA	1 cut(s)	326
AvaI	CYCGRG	1 cut(s)	730
BanI	GGYRCC	2 cut(s)	413, 994
BbsI	GAAGAC	2 cut(s)	777, 941
BbvI	GCAGC	1 cut(s)	755
BccI	CCATC	1 cut(s)	931
BceAI	ACGGC	2 cut(s)	427, 735
BcgI	CGANNNNNNTGC	6 cut(s)	49, 74, 83, 108, 787, 821
BciT130I	CCWGG	4 cut(s)	291, 519, 900, 928
BciVI	GTATCC	2 cut(s)	598, 1036
BcoDI	GTCTC	1 cut(s)	614
BfmI	CTRYAG	2 cut(s)	450, 577
BfoI	RGCGCY	1 cut(s)	548
BfuI	GTATCC	2 cut(s)	598, 1036
BglI	GCCNNNNNGGC	1 cut(s)	461
BisI	GCNGC	1 cut(s)	744
BlsI	GCNGC	1 cut(s)	745
Bme1390I	CCNGG	4 cut(s)	291, 519, 900, 928
BmeT110I	CYCGRG	1 cut(s)	730
BmiI	GGNNCC	5 cut(s)	249, 353, 415, 811, 996
BmrFI	CCNGG	4 cut(s)	291, 519, 900, 928
BmrI	ACTGGG	1 cut(s)	492
BmsI	GCATC	1 cut(s)	407
BmuI	ACTGGG	1 cut(s)	492
BpiI	GAAGAC	2 cut(s)	777, 941
BpmI	CTGGAG	3 cut(s)	540, 882, 1080
Bpu14I	TTCGAA	1 cut(s)	326
BsaBI	GATNNNNATC	1 cut(s)	688
BsaJI	CCNNGG	2 cut(s)	899, 998
BsaWI	WCCGGW	2 cut(s)	250, 416
Bsc4I	CCNNNNNNNGG	1 cut(s)	142
Bse118I	RCCGGY	2 cut(s)	416, 812
Bse1I	ACTGG	4 cut(s)	487, 628, 700, 917
Bse3DI	GCAATG	1 cut(s)	1073
Bse8I	GATNNNNATC	1 cut(s)	688
BseAI	TCCGGA	1 cut(s)	250
BseBI	CCWGG	4 cut(s)	291, 519, 900, 928
BseDI	CCNNGG	2 cut(s)	899, 998
BseGI	GGATG	3 cut(s)	121, 136, 923
BseJI	GATNNNNATC	1 cut(s)	688
BseLI	CCNNNNNNNGG	1 cut(s)	142
BseMI	GCAATG	1 cut(s)	1073
BseMII	CTCAG	1 cut(s)	714
BseNI	ACTGG	4 cut(s)	487, 628, 700, 917
BseRI	GAGGAG	5 cut(s)	222, 225, 885, 987, 1037
BseXI	GCAGC	1 cut(s)	755
BseYI	CCCAGC	1 cut(s)	111
Bsh1236I	CGCG	1 cut(s)	168
BshFI	GGCC	1 cut(s)	1033
BshNI	GGYRCC	2 cut(s)	413, 994
BshTI	ACCGGT	1 cut(s)	416
BsiHKCI	CYCGRG	1 cut(s)	730
BsiSI	CCGG	4 cut(s)	181, 251, 417, 813
BslFI	GGGAC	2 cut(s)	610, 770
BslI	CCNNNNNNNGG	1 cut(s)	142
BsmAI	GTCTC	1 cut(s)	614
BsmFI	GGGAC	2 cut(s)	610, 770
BsmI	GAATGC	1 cut(s)	537
BsnI	GGCC	1 cut(s)	1033
BsoBI	CYCGRG	1 cut(s)	730
Bsp119I	TTCGAA	1 cut(s)	326
Bsp13I	TCCGGA	1 cut(s)	250
Bsp143I	GATC	5 cut(s)	183, 486, 626, 683, 689
Bsp19I	CCATGG	1 cut(s)	998
BspACI	CCGC	3 cut(s)	557, 857, 987
BspANI	GGCC	1 cut(s)	1033
BspCNI	CTCAG	1 cut(s)	715
BspEI	TCCGGA	1 cut(s)	250
BspFNI	CGCG	1 cut(s)	168
BspLI	GGNNCC	5 cut(s)	249, 353, 415, 811, 996
BspPI	GGATC	3 cut(s)	191, 494, 634
BspQI	GCTCTTC	1 cut(s)	826
BspT104I	TTCGAA	1 cut(s)	326
BspT107I	GGYRCC	2 cut(s)	413, 994
BsrBI	CCGCTC	1 cut(s)	857
BsrDI	GCAATG	1 cut(s)	1073
BsrFI	RCCGGY	2 cut(s)	416, 812
BsrI	ACTGG	4 cut(s)	487, 628, 700, 917
BssAI	RCCGGY	2 cut(s)	416, 812
BssECI	CCNNGG	2 cut(s)	899, 998
BssMI	GATC	5 cut(s)	183, 486, 626, 683, 689
BssT1I	CCWWGG	1 cut(s)	998
Bst2UI	CCWGG	4 cut(s)	291, 519, 900, 928
Bst4CI	ACNGT	8 cut(s)	137, 226, 376, 505, 647, 765, 872, 940
Bst6I	CTCTTC	1 cut(s)	826
BstAPI	GCANNNNNTGC	1 cut(s)	420
BstBI	TTCGAA	1 cut(s)	326
BstC8I	GCNNGC	1 cut(s)	537
BstDEI	CTNAG	3 cut(s)	548, 723, 1073
BstDSI	CCRYGG	1 cut(s)	998
BstF5I	GGATG	3 cut(s)	121, 136, 923
BstFNI	CGCG	1 cut(s)	168
BstH2I	RGCGCY	1 cut(s)	548
BstHHI	GCGC	2 cut(s)	479, 547
BstKTI	GATC	5 cut(s)	186, 489, 629, 686, 692
BstMAI	GTCTC	1 cut(s)	614
BstMBI	GATC	5 cut(s)	183, 486, 626, 683, 689
BstMWI	GCNNNNNNNGC	5 cut(s)	420, 452, 461, 607, 743
BstNI	CCWGG	4 cut(s)	291, 519, 900, 928
BstSCI	CCNGG	4 cut(s)	289, 517, 898, 926
BstSFI	CTRYAG	2 cut(s)	450, 577
BstUI	CGCG	1 cut(s)	168
BstV1I	GCAGC	1 cut(s)	755
BstV2I	GAAGAC	2 cut(s)	777, 941
BstX2I	RGATCY	1 cut(s)	626
BstYI	RGATCY	1 cut(s)	626
BsuI	GTATCC	2 cut(s)	598, 1036
BsuRI	GGCC	1 cut(s)	1033
BtgI	CCRYGG	1 cut(s)	998
BtsCI	GGATG	3 cut(s)	121, 136, 923
BtsI	GCAGTG	2 cut(s)	519, 546
BtsIMutI	CAGTG	7 cut(s)	16, 510, 519, 546, 643, 707, 945
Cac8I	GCNNGC	1 cut(s)	537
CaiI	CAGNNNCTG	1 cut(s)	518
CfoI	GCGC	2 cut(s)	479, 547
Cfr10I	RCCGGY	2 cut(s)	416, 812
CseI	GACGC	1 cut(s)	607
Csp6I	GTAC	2 cut(s)	311, 317
CspAI	ACCGGT	1 cut(s)	416
CviAII	CATG	3 cut(s)	244, 571, 999
CviQI	GTAC	2 cut(s)	311, 317
DdeI	CTNAG	3 cut(s)	548, 723, 1073
DpnI	GATC	5 cut(s)	185, 488, 628, 685, 691
DpnII	GATC	5 cut(s)	183, 486, 626, 683, 689
Eam1104I	CTCTTC	1 cut(s)	826
EarI	CTCTTC	1 cut(s)	826
Eco130I	CCWWGG	1 cut(s)	998
Eco147I	AGGCCT	1 cut(s)	1033
Eco88I	CYCGRG	1 cut(s)	730
EcoRII	CCWGG	4 cut(s)	289, 517, 898, 926
EcoT14I	CCWWGG	1 cut(s)	998
ErhI	CCWWGG	1 cut(s)	998
FaeI	CATG	3 cut(s)	247, 574, 1002
FaqI	GGGAC	2 cut(s)	610, 770
FatI	CATG	3 cut(s)	243, 570, 998
FauI	CCCGC	2 cut(s)	550, 980
Fnu4HI	GCNGC	1 cut(s)	744
FokI	GGATG	3 cut(s)	128, 143, 910
Fsp4HI	GCNGC	1 cut(s)	744
FspI	TGCGCA	1 cut(s)	478
GlaI	GCGC	2 cut(s)	478, 546
GluI	GCNGC	1 cut(s)	744
GsaI	CCCAGC	1 cut(s)	115
GsuI	CTGGAG	3 cut(s)	540, 882, 1080
HaeII	RGCGCY	1 cut(s)	548
HaeIII	GGCC	1 cut(s)	1033
HapII	CCGG	4 cut(s)	181, 251, 417, 813
HgaI	GACGC	1 cut(s)	607
HhaI	GCGC	2 cut(s)	479, 547
Hin1II	CATG	3 cut(s)	247, 574, 1002
Hin6I	GCGC	2 cut(s)	477, 545
HinP1I	GCGC	2 cut(s)	477, 545
HincII	GTYRAC	2 cut(s)	196, 241
HindII	GTYRAC	2 cut(s)	196, 241
HinfI	GANTC	6 cut(s)	6, 86, 215, 270, 758, 780
HpaII	CCGG	4 cut(s)	181, 251, 417, 813
HphI	GGTGA	3 cut(s)	481, 572, 753
Hpy166II	GTNNAC	4 cut(s)	196, 241, 667, 793
Hpy188I	TCNGA	5 cut(s)	370, 403, 431, 809, 950
Hpy188III	TCNNGA	7 cut(s)	251, 496, 687, 732, 852, 1040, 1059
Hpy8I	GTNNAC	4 cut(s)	196, 241, 667, 793
HpyAV	CCTTC	3 cut(s)	118, 301, 502
HpyCH4III	ACNGT	8 cut(s)	137, 226, 376, 505, 647, 765, 872, 940
HpyCH4V	TGCA	5 cut(s)	95, 512, 535, 798, 963
HpyF10VI	GCNNNNNNNGC	5 cut(s)	420, 452, 461, 607, 743
HpyF3I	CTNAG	3 cut(s)	548, 723, 1073
Hsp92II	CATG	3 cut(s)	247, 574, 1002
HspAI	GCGC	2 cut(s)	477, 545
Kpn2I	TCCGGA	1 cut(s)	250
Kzo9I	GATC	5 cut(s)	183, 486, 626, 683, 689
LguI	GCTCTTC	1 cut(s)	826
LmnI	GCTCC	4 cut(s)	233, 734, 906, 974
Lsp1109I	GCAGC	1 cut(s)	755
LweI	GCATC	1 cut(s)	407
MaeIII	GTNAC	2 cut(s)	560, 647
MalI	GATC	5 cut(s)	185, 488, 628, 685, 691
MbiI	CCGCTC	1 cut(s)	857
MboI	GATC	5 cut(s)	183, 486, 626, 683, 689
MboII	GAAGA	5 cut(s)	173, 278, 782, 843, 946
MflI	RGATCY	1 cut(s)	626
MluCI	AATT	5 cut(s)	100, 276, 294, 844, 1049
MluI	ACGCGT	1 cut(s)	166
MlyI	GAGTC	1 cut(s)	752
MnlI	CCTC	8 cut(s)	200, 203, 454, 515, 906, 965, 984, 1015
MroI	TCCGGA	1 cut(s)	250
MroXI	GAANNNNTTC	3 cut(s)	445, 847, 1010
MseI	TTAA	2 cut(s)	63, 171
MslI	CAYNNNNRTG	1 cut(s)	879
MspA1I	CMGCKG	1 cut(s)	111
MspI	CCGG	4 cut(s)	181, 251, 417, 813
MspR9I	CCNGG	4 cut(s)	291, 519, 900, 928
Mva1269I	GAATGC	1 cut(s)	537
MvaI	CCWGG	4 cut(s)	291, 519, 900, 928
MvnI	CGCG	1 cut(s)	168
MwoI	GCNNNNNNNGC	5 cut(s)	420, 452, 461, 607, 743
NcoI	CCATGG	1 cut(s)	998
NdeII	GATC	5 cut(s)	183, 486, 626, 683, 689
NlaIII	CATG	3 cut(s)	247, 574, 1002
NlaIV	GGNNCC	5 cut(s)	249, 353, 415, 811, 996
NmuCI	GTSAC	2 cut(s)	560, 647
NsbI	TGCGCA	1 cut(s)	478
NspV	TTCGAA	1 cut(s)	326
PceI	AGGCCT	1 cut(s)	1033
PciSI	GCTCTTC	1 cut(s)	826
PctI	GAATGC	1 cut(s)	537
PdmI	GAANNNNTTC	3 cut(s)	445, 847, 1010
PfeI	GAWTC	5 cut(s)	6, 86, 215, 270, 780
PfoI	TCCNGGA	1 cut(s)	289
PinAI	ACCGGT	1 cut(s)	416
PkrI	GCNGC	1 cut(s)	745
PleI	GAGTC	1 cut(s)	752
PpsI	GAGTC	1 cut(s)	752
PsiI	TTATAA	1 cut(s)	1081
Psp6I	CCWGG	4 cut(s)	289, 517, 898, 926
PspFI	CCCAGC	1 cut(s)	111
PspGI	CCWGG	4 cut(s)	289, 517, 898, 926
PspN4I	GGNNCC	5 cut(s)	249, 353, 415, 811, 996
PstNI	CAGNNNCTG	1 cut(s)	518
PsuI	RGATCY	1 cut(s)	626
PvuII	CAGCTG	1 cut(s)	111
RsaI	GTAC	2 cut(s)	312, 318
RsaNI	GTAC	2 cut(s)	311, 317
RseI	CAYNNNNRTG	1 cut(s)	879
SapI	GCTCTTC	1 cut(s)	826
SaqAI	TTAA	2 cut(s)	63, 171
SatI	GCNGC	1 cut(s)	744
Sau3AI	GATC	5 cut(s)	183, 486, 626, 683, 689
SchI	GAGTC	1 cut(s)	752
ScrFI	CCNGG	4 cut(s)	291, 519, 900, 928
SetI	ASST	8 cut(s)	69, 113, 129, 312, 494, 748, 838, 1019
SfaNI	GCATC	1 cut(s)	407
SfcI	CTRYAG	2 cut(s)	450, 577
SfuI	TTCGAA	1 cut(s)	326
SmiMI	CAYNNNNRTG	1 cut(s)	879
Sse9I	AATT	5 cut(s)	100, 276, 294, 844, 1049
SseBI	AGGCCT	1 cut(s)	1033
SsiI	CCGC	3 cut(s)	557, 857, 987
StuI	AGGCCT	1 cut(s)	1033
StyD4I	CCNGG	4 cut(s)	289, 517, 898, 926
StyI	CCWWGG	1 cut(s)	998
TaaI	ACNGT	8 cut(s)	137, 226, 376, 505, 647, 765, 872, 940
TaqI	TCGA	3 cut(s)	326, 495, 879
TasI	AATT	5 cut(s)	100, 276, 294, 844, 1049
TfiI	GAWTC	5 cut(s)	6, 86, 215, 270, 780
Tru1I	TTAA	2 cut(s)	63, 171
Tru9I	TTAA	2 cut(s)	63, 171
TscAI	CASTG	7 cut(s)	16, 510, 519, 546, 650, 707, 945
TseFI	GTSAC	2 cut(s)	560, 647
TseI	GCWGC	1 cut(s)	743
Tsp45I	GTSAC	2 cut(s)	560, 647
TspDTI	ATGAA	2 cut(s)	661, 783
TspRI	CASTG	7 cut(s)	16, 510, 519, 546, 650, 707, 945
XapI	RAATTY	3 cut(s)	276, 294, 1049
XmnI	GAANNNNTTC	3 cut(s)	445, 847, 1010

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.