MD11G1201900.v1.1

Pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0013350
Physical Location & Seq
Reverse (-)
29222428 .. 29224192
1765 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1201900.v1.1.491

Sequence Viewer

Length: 1086 bp
ATGGAATTGGGTGTCTGGAGTGTTTTTGTTGTTGGTTTTGTGTTGCTGTTCTTGGGTTGCATTGAGGTCGGGTTCTCACAAAGCGAAGCGTATGCAAGGAATTATATCAGCTGGGATGATTTGGAGGTGGATGACCATAAGTTGGGGTTGAACACAAGAGAAGAACACGTTAATGGAAGCCGGATTATTGTGGTCGACAAGAATGGAGGAGGAAATTCTCTAACAGTTCAAGGAGCAGTTGATATGGTTCCGGAACAAAATACAGAAAGAGTCAAAATTTACATTCTTCCTGGAATTTACAGAGAGAAGGTACTTGTACCGATTTCGAAGCCGTACATATCATTTATTGGGAATCCGAATCAAACTTCTGAAACTGTGATTACTTGGAACAACAAGGCATCCGATAAGGACGGCCTTCGTTTCGAACTGGGAACTTACAGAACTGCTTCTGTAGCCATAGAGGCCGATTACTTTTGCGCAACAGGGATCACCTTCGAGAACTCGGTGGTTGCAGTGCCTGGAGGATATGGAATGCAAGCAGTGGCTCTTAGAGTTGCCGGTGACAAAGCCATGTTCTACAGAGTTCGGGTTTTGGGGACGCAGGATACACTCCTGGACGACACTGGATCTCACTACTTCTACCGATGTCACATTCAAGGAAGTGTGGACTTCATTTTTGGCAGATCAAGATCACTCTACCAGGACTGTGTTATTCAATCAACGGCTCAGAACTCGGGAGCAATAGCGGCTCATCACCGGGACTCGCCGAATGAAGACACAGGATTCTCCTTTGTAAACTGCAAAATCATCGGGACTGGCTACATCTACTTGGGAAGAGCTTGGGGAAATTATTCAAGAACGGTGTATTCCTACTGTCATTTCGATGATATAATAACTCCACCGGGGTGGAGCGACTGGAACTACCCATCCAGGCAGAAGACAGTGGATTTCGGAGAATACGAATGCAGGGGCAGAGGAGCAGAAAGAAGAGGGAGGGTGCCATGGTTGAAGTCTTTCAGCTTGGAGGAGATAAGGCCTTTTCTGGATACAAAATTTATATCTGGAGAGCAGTGGCTTAGATTATAA

Protein Analysis

362

Amino Acids

40.65

Weight (kDa)

5.71

Isoelectric Point (pI)

32.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 63 - 355 1.9e-65 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013350)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G55590 AT5G55590
fragaria_vesca FvH4_3g29490
malus_domestica MD03G1186900.v1.1 MD11G1201900.v1.1
prunus_persica Prupe.4G239700_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0054371
rosa_laevigata RLG00000034927
rosa_multiflora Rmu_sc0005705.1_g000023 Rmu_sc0023043.1_g000001
rosa_roxburghii Rroxscaffold_1G00026500
rosa_rugosa Rorug05G0285900
rosa_samantha Rh5AG356400 Rh5BG368000 Rh5CG391300 Rh5DG382200
rosa_wichuraiana Rw5G033480 Rw5G033650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1084
Acc16I TGCGCA 1 cut(s) 478
AccB1I GGYRCC 1 cut(s) 997
AccB7I CCANNNNNTGG 1 cut(s) 142
AccI GTMKAC 1 cut(s) 195
AccIII TCCGGA 1 cut(s) 250
AciI CCGC 1 cut(s) 746
AclWI GGATC 2 cut(s) 494, 634
AcsI RAATTY 4 cut(s) 214, 276, 294, 1052
AfaI GTAC 3 cut(s) 312, 318, 335
AfiI CCNNNNNNNGG 1 cut(s) 142
AflIII ACRYGT 1 cut(s) 166
AgsI TTSAA 6 cut(s) 151, 230, 656, 716, 855, 1009
AjnI CCWGG 5 cut(s) 289, 517, 612, 699, 929
AleI CACNNNNGTG 1 cut(s) 904
AloI GAACNNNNNNTCC 2 cut(s) 911, 943
AluBI AGCT 3 cut(s) 111, 839, 1020
AluI AGCT 3 cut(s) 111, 839, 1020
AlwI GGATC 2 cut(s) 494, 634
AlwNI CAGNNNCTG 1 cut(s) 518
Ama87I CYCGRG 1 cut(s) 733
Aor13HI TCCGGA 1 cut(s) 250
AoxI GGCC 3 cut(s) 412, 462, 1034
ApoI RAATTY 4 cut(s) 214, 276, 294, 1052
ArsI GACNNNNNNTTYG 2 cut(s) 659, 691
Asp700I GAANNNNTTC 3 cut(s) 445, 850, 1013
AspLEI GCGC 1 cut(s) 479
AsuC2I CCSGG 2 cut(s) 758, 903
AsuHPI GGTGA 3 cut(s) 481, 572, 746
AsuII TTCGAA 2 cut(s) 326, 423
AvaI CYCGRG 1 cut(s) 733
BanI GGYRCC 1 cut(s) 997
BbsI GAAGAC 2 cut(s) 780, 944
BccI CCATC 1 cut(s) 934
BceAI ACGGC 3 cut(s) 316, 427, 738
BcgI CGANNNNNNTGC 6 cut(s) 49, 74, 83, 108, 790, 824
BciT130I CCWGG 5 cut(s) 291, 519, 614, 701, 931
BciVI GTATCC 2 cut(s) 598, 1039
BcnI CCSGG 2 cut(s) 758, 903
BfmI CTRYAG 2 cut(s) 450, 577
BfuI GTATCC 2 cut(s) 598, 1039
BglI GCCNNNNNGGC 1 cut(s) 461
BisI GCNGC 1 cut(s) 747
BlsI GCNGC 1 cut(s) 748
Bme1390I CCNGG 7 cut(s) 291, 519, 614, 701, 758, 903, 931
BmeT110I CYCGRG 1 cut(s) 733
BmiI GGNNCC 2 cut(s) 249, 999
BmrFI CCNGG 7 cut(s) 291, 519, 614, 701, 758, 903, 931
BmrI ACTGGG 1 cut(s) 437
BmsI GCATC 1 cut(s) 407
BmuI ACTGGG 1 cut(s) 437
BpiI GAAGAC 2 cut(s) 780, 944
BpmI CTGGAG 3 cut(s) 37, 540, 1083
Bpu14I TTCGAA 2 cut(s) 326, 423
BpuMI CCSGG 2 cut(s) 758, 903
BsaBI GATNNNNATC 1 cut(s) 688
BsaJI CCNNGG 2 cut(s) 902, 1001
BsaWI WCCGGW 1 cut(s) 250
Bsc4I CCNNNNNNNGG 1 cut(s) 142
Bse118I RCCGGY 1 cut(s) 557
Bse1I ACTGG 4 cut(s) 432, 628, 820, 920
Bse8I GATNNNNATC 1 cut(s) 688
BseAI TCCGGA 1 cut(s) 250
BseBI CCWGG 5 cut(s) 291, 519, 614, 701, 931
BseDI CCNNGG 2 cut(s) 902, 1001
BseGI GGATG 4 cut(s) 121, 136, 398, 926
BseJI GATNNNNATC 1 cut(s) 688
BseLI CCNNNNNNNGG 1 cut(s) 142
BseMII CTCAG 1 cut(s) 740
BseNI ACTGG 4 cut(s) 432, 628, 820, 920
BseRI GAGGAG 3 cut(s) 222, 990, 1040
BseYI CCCAGC 1 cut(s) 111
BshFI GGCC 3 cut(s) 414, 464, 1036
BshNI GGYRCC 1 cut(s) 997
BsiHKCI CYCGRG 1 cut(s) 733
BsiSI CCGG 5 cut(s) 181, 251, 558, 757, 902
BslFI GGGAC 3 cut(s) 610, 773, 826
BslI CCNNNNNNNGG 1 cut(s) 142
BsmFI GGGAC 3 cut(s) 610, 773, 826
BsmI GAATGC 2 cut(s) 537, 968
BsnI GGCC 3 cut(s) 414, 464, 1036
BsoBI CYCGRG 1 cut(s) 733
Bsp119I TTCGAA 2 cut(s) 326, 423
Bsp13I TCCGGA 1 cut(s) 250
Bsp143I GATC 4 cut(s) 486, 626, 683, 689
Bsp19I CCATGG 1 cut(s) 1001
BspACI CCGC 1 cut(s) 746
BspANI GGCC 3 cut(s) 414, 464, 1036
BspCNI CTCAG 1 cut(s) 739
BspEI TCCGGA 1 cut(s) 250
BspLI GGNNCC 2 cut(s) 249, 999
BspPI GGATC 2 cut(s) 494, 634
BspQI GCTCTTC 1 cut(s) 829
BspT104I TTCGAA 2 cut(s) 326, 423
BspT107I GGYRCC 1 cut(s) 997
BsrFI RCCGGY 1 cut(s) 557
BsrI ACTGG 4 cut(s) 432, 628, 820, 920
BssAI RCCGGY 1 cut(s) 557
BssECI CCNNGG 2 cut(s) 902, 1001
BssMI GATC 4 cut(s) 486, 626, 683, 689
BssT1I CCWWGG 1 cut(s) 1001
Bst2UI CCWGG 5 cut(s) 291, 519, 614, 701, 931
Bst4CI ACNGT 6 cut(s) 226, 376, 707, 862, 875, 943
Bst6I CTCTTC 2 cut(s) 829, 982
BstBI TTCGAA 2 cut(s) 326, 423
BstC8I GCNNGC 1 cut(s) 537
BstDEI CTNAG 3 cut(s) 548, 726, 1076
BstDSI CCRYGG 1 cut(s) 1001
BstF5I GGATG 4 cut(s) 121, 136, 398, 926
BstHHI GCGC 1 cut(s) 479
BstKTI GATC 4 cut(s) 489, 629, 686, 692
BstMBI GATC 4 cut(s) 486, 626, 683, 689
BstMWI GCNNNNNNNGC 3 cut(s) 452, 461, 746
BstNI CCWGG 5 cut(s) 291, 519, 614, 701, 931
BstSCI CCNGG 7 cut(s) 289, 517, 612, 699, 756, 901, 929
BstSFI CTRYAG 2 cut(s) 450, 577
BstV2I GAAGAC 2 cut(s) 780, 944
BstX2I RGATCY 1 cut(s) 626
BstXI CCANNNNNNTGG 1 cut(s) 906
BstYI RGATCY 1 cut(s) 626
BsuI GTATCC 2 cut(s) 598, 1039
BsuRI GGCC 3 cut(s) 414, 464, 1036
BtgI CCRYGG 1 cut(s) 1001
BtsCI GGATG 4 cut(s) 121, 136, 398, 926
BtsI GCAGTG 3 cut(s) 519, 546, 1076
BtsIMutI CAGTG 5 cut(s) 519, 546, 621, 948, 1076
Cac8I GCNNGC 1 cut(s) 537
CaiI CAGNNNCTG 1 cut(s) 518
CfoI GCGC 1 cut(s) 479
Cfr10I RCCGGY 1 cut(s) 557
CseI GACGC 1 cut(s) 607
Csp6I GTAC 3 cut(s) 311, 317, 334
CviAII CATG 2 cut(s) 571, 1002
CviQI GTAC 3 cut(s) 311, 317, 334
DdeI CTNAG 3 cut(s) 548, 726, 1076
DpnI GATC 4 cut(s) 488, 628, 685, 691
DpnII GATC 4 cut(s) 486, 626, 683, 689
Eam1104I CTCTTC 2 cut(s) 829, 982
EarI CTCTTC 2 cut(s) 829, 982
Eco130I CCWWGG 1 cut(s) 1001
Eco147I AGGCCT 1 cut(s) 1036
Eco88I CYCGRG 1 cut(s) 733
EcoRII CCWGG 5 cut(s) 289, 517, 612, 699, 929
EcoT14I CCWWGG 1 cut(s) 1001
ErhI CCWWGG 1 cut(s) 1001
FaeI CATG 2 cut(s) 574, 1005
FaqI GGGAC 3 cut(s) 610, 773, 826
FatI CATG 2 cut(s) 570, 1001
FblI GTMKAC 1 cut(s) 195
Fnu4HI GCNGC 1 cut(s) 747
FokI GGATG 4 cut(s) 128, 143, 385, 913
Fsp4HI GCNGC 1 cut(s) 747
FspI TGCGCA 1 cut(s) 478
GlaI GCGC 1 cut(s) 478
GluI GCNGC 1 cut(s) 747
GsaI CCCAGC 1 cut(s) 115
GsuI CTGGAG 3 cut(s) 37, 540, 1083
HaeIII GGCC 3 cut(s) 414, 464, 1036
HapII CCGG 5 cut(s) 181, 251, 558, 757, 902
HgaI GACGC 1 cut(s) 607
HhaI GCGC 1 cut(s) 479
Hin1II CATG 2 cut(s) 574, 1005
Hin6I GCGC 1 cut(s) 477
HinP1I GCGC 1 cut(s) 477
HincII GTYRAC 1 cut(s) 196
HindII GTYRAC 1 cut(s) 196
HinfI GANTC 5 cut(s) 270, 352, 358, 761, 783
HpaII CCGG 5 cut(s) 181, 251, 558, 757, 902
HphI GGTGA 3 cut(s) 481, 572, 746
Hpy166II GTNNAC 3 cut(s) 196, 667, 796
Hpy188I TCNGA 5 cut(s) 357, 370, 403, 729, 953
Hpy188III TCNNGA 9 cut(s) 16, 251, 496, 687, 735, 811, 855, 1043, 1062
Hpy8I GTNNAC 3 cut(s) 196, 667, 796
HpyAV CCTTC 3 cut(s) 301, 425, 502
HpyCH4III ACNGT 6 cut(s) 226, 376, 707, 862, 875, 943
HpyCH4IV ACGT 1 cut(s) 168
HpyCH4V TGCA 6 cut(s) 60, 95, 512, 535, 801, 966
HpyF10VI GCNNNNNNNGC 3 cut(s) 452, 461, 746
HpyF3I CTNAG 3 cut(s) 548, 726, 1076
HpySE526I ACGT 1 cut(s) 168
Hsp92II CATG 2 cut(s) 574, 1005
HspAI GCGC 1 cut(s) 477
Kpn2I TCCGGA 1 cut(s) 250
Kzo9I GATC 4 cut(s) 486, 626, 683, 689
LguI GCTCTTC 1 cut(s) 829
LmnI GCTCC 4 cut(s) 233, 737, 909, 977
LweI GCATC 1 cut(s) 407
MaeII ACGT 1 cut(s) 168
MaeIII GTNAC 2 cut(s) 560, 647
MalI GATC 4 cut(s) 488, 628, 685, 691
MboI GATC 4 cut(s) 486, 626, 683, 689
MboII GAAGA 6 cut(s) 173, 278, 785, 846, 949, 999
MflI RGATCY 1 cut(s) 626
MluCI AATT 7 cut(s) 5, 100, 214, 276, 294, 847, 1052
MlyI GAGTC 2 cut(s) 279, 755
MroI TCCGGA 1 cut(s) 250
MroXI GAANNNNTTC 3 cut(s) 445, 850, 1013
MseI TTAA 1 cut(s) 171
MslI CAYNNNNRTG 3 cut(s) 171, 882, 904
MspA1I CMGCKG 1 cut(s) 111
MspI CCGG 5 cut(s) 181, 251, 558, 757, 902
MspR9I CCNGG 7 cut(s) 291, 519, 614, 701, 758, 903, 931
Mva1269I GAATGC 2 cut(s) 537, 968
MvaI CCWGG 5 cut(s) 291, 519, 614, 701, 931
MwoI GCNNNNNNNGC 3 cut(s) 452, 461, 746
NciI CCSGG 2 cut(s) 758, 903
NcoI CCATGG 1 cut(s) 1001
NdeII GATC 4 cut(s) 486, 626, 683, 689
NlaIII CATG 2 cut(s) 574, 1005
NlaIV GGNNCC 2 cut(s) 249, 999
NmuCI GTSAC 2 cut(s) 560, 647
NsbI TGCGCA 1 cut(s) 478
NspV TTCGAA 2 cut(s) 326, 423
OliI CACNNNNGTG 1 cut(s) 904
PceI AGGCCT 1 cut(s) 1036
PciSI GCTCTTC 1 cut(s) 829
PcsI WCGNNNNNNNCGW 1 cut(s) 957
PctI GAATGC 2 cut(s) 537, 968
PdmI GAANNNNTTC 3 cut(s) 445, 850, 1013
PfeI GAWTC 3 cut(s) 352, 358, 783
PflMI CCANNNNNTGG 1 cut(s) 142
PfoI TCCNGGA 2 cut(s) 289, 612
PkrI GCNGC 1 cut(s) 748
PleI GAGTC 2 cut(s) 278, 755
PpsI GAGTC 2 cut(s) 278, 755
PsiI TTATAA 1 cut(s) 1084
Psp6I CCWGG 5 cut(s) 289, 517, 612, 699, 929
PspFI CCCAGC 1 cut(s) 111
PspGI CCWGG 5 cut(s) 289, 517, 612, 699, 929
PspN4I GGNNCC 2 cut(s) 249, 999
PstNI CAGNNNCTG 1 cut(s) 518
PsuI RGATCY 1 cut(s) 626
PvuII CAGCTG 1 cut(s) 111
RsaI GTAC 3 cut(s) 312, 318, 335
RsaNI GTAC 3 cut(s) 311, 317, 334
RseI CAYNNNNRTG 3 cut(s) 171, 882, 904
SalI GTCGAC 1 cut(s) 194
SapI GCTCTTC 1 cut(s) 829
SaqAI TTAA 1 cut(s) 171
SatI GCNGC 1 cut(s) 747
Sau3AI GATC 4 cut(s) 486, 626, 683, 689
SchI GAGTC 2 cut(s) 279, 755
ScrFI CCNGG 7 cut(s) 291, 519, 614, 701, 758, 903, 931
SetI ASST 8 cut(s) 69, 113, 129, 171, 312, 494, 841, 1022
SfaNI GCATC 1 cut(s) 407
SfcI CTRYAG 2 cut(s) 450, 577
SfuI TTCGAA 2 cut(s) 326, 423
SmiMI CAYNNNNRTG 3 cut(s) 171, 882, 904
Sse9I AATT 7 cut(s) 5, 100, 214, 276, 294, 847, 1052
SseBI AGGCCT 1 cut(s) 1036
SsiI CCGC 1 cut(s) 746
StuI AGGCCT 1 cut(s) 1036
StyD4I CCNGG 7 cut(s) 289, 517, 612, 699, 756, 901, 929
StyI CCWWGG 1 cut(s) 1001
TaaI ACNGT 6 cut(s) 226, 376, 707, 862, 875, 943
TaiI ACGT 1 cut(s) 171
TaqI TCGA 5 cut(s) 195, 326, 423, 495, 882
TasI AATT 7 cut(s) 5, 100, 214, 276, 294, 847, 1052
TauI GCSGC 1 cut(s) 749
TfiI GAWTC 3 cut(s) 352, 358, 783
Tru1I TTAA 1 cut(s) 171
Tru9I TTAA 1 cut(s) 171
TscAI CASTG 5 cut(s) 519, 546, 628, 948, 1076
TseFI GTSAC 2 cut(s) 560, 647
Tsp45I GTSAC 2 cut(s) 560, 647
TspDTI ATGAA 2 cut(s) 661, 786
TspRI CASTG 5 cut(s) 519, 546, 628, 948, 1076
Van91I CCANNNNNTGG 1 cut(s) 142
XapI RAATTY 4 cut(s) 214, 276, 294, 1052
XmiI GTMKAC 1 cut(s) 195
XmnI GAANNNNTTC 3 cut(s) 445, 850, 1013
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.