Rorug05G0285900

Pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0013350
Physical Location & Seq
Reverse (-)
32814549 .. 32816137
1589 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0285900.1

Sequence Viewer

Length: 1503 bp
ATGGTGATGGAGTGGACTAGAGGACCAACCATCGGCCGTGGCTCCACCGCCACCGTCTCCCTAGCCACTGCAGTCCTCTCCGGTGAGGTCTTCGCGGTGAAGTCGGCTGAGCTTTCGCGGTCGTCGTTCCTGCGAAAGGAGCAGTGTTTTCTATCTAAGCTGAGCTCTCCTCATGTGGTCAAGTACTTGGGTTGTGATGTTAGCACTGAACACAATGAGCCTATGTACAATCTTTTCATGGAGTACGTACCCGGCGGCACGCTTTCCGATGCGATTCGAAGACATGGAAAGGAGCTCGAGGAGTGTATGATCCAATCATACACGCATCAGATTGTGCAGGGTTTGGAATATCTTCATTCAATTGGGTTGGCACATTGTGACATTAAGAGCCAGAACATTTTGGTGTCTGAAGAGAGTGCCCAGATCAAAATTGCTGATCTGGGTTGTGCTAAATTGGTGCATGAAGCATCCACGTCGGCATTTTCCGGCACGCCGGTGTTCATGGCGCCGGAGGTGGCACGCGGAGAAGAGCAGGGTTTTGAAGCAGATATATGGGCACTTGGCTGTACATTGATTGAAATGGCCACAGGGGGCAATAGCCCCTGGCCGGAGGTAAGTGACCCTGTTTCGGCTCTCTACCGGATTGGATTCTCCGGTGACTTGCCGGAGTTTCCGAGGTGGCTTTCGAAAGAGGGTAAGGATTTCTTGAGCCAGTGTTTGACGACATGCCCAAGAGAGAGGTGGACAGCTAAGAAGCTTCTTGAGCATCCATTTCTTAAAGACTACTCAATGGGGTCTACGACTCTGAGCTCATCTCCTATTAGTGTGTTGGATCAAGGCCTTTGGGATTCATTTGAAGTCTTTGAAGAAGCTCCTCAGAATCAAGTTTGTGAAAGCATTTGTTCCAGTTCTTCTCCGCAAGAAAGGATCAAGATGTTGATCGGAGGTACTTGTTCTACATTTTCAGGTATACCCAATTGGGAATTTGATGAGAATTGGTGCACTGTTAGAAGCAATGGTGCCGAGGATAGCGAAGCCTTATTGAAGAACAACAATGTCATTGTTGCTACTTCAGAGCAGGAATATATGGCGGCAACTGTTGCATCAGATGAGAAGCTTCACAGTTCAATGTGTGATGATGAAGAAGATGATGATGAGGAATTAGATTCTGAATGTTTTGTAGAGTCTAACAGTAGAATTGGTAGTGATGGAGATTTTGTAATTTCATACATTGTTACAGACGATTCTTTCGTAAACAATCTTGATATTGAACAAGATACTCGGAATTATTCTTTTATTCACTTCCATTGTTATCATTCGTGTTTTGATTTTTCTGGCCAAACTGTGGTGCTTAAGTTTCTCTTGTTAATGTCTTCTCTGTTTTGTGTTAGTTTGGTACAATGCCACATGACAGATCAATTTCTCGATCTACAGGGTAAATGCATATATGTGAAGGGCGAAATGCCTTACTCGAGCATAAGGATAAATGCTCTTAGCAACTAG

Protein Analysis

500

Amino Acids

55.56

Weight (kDa)

4.58

Isoelectric Point (pI)

49.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 7 - 259 7.3e-57 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 10 - 255 1.5e-35 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013350)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G55590 AT5G55590
fragaria_vesca FvH4_3g29490
malus_domestica MD03G1186900.v1.1 MD11G1201900.v1.1
prunus_persica Prupe.4G239700_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0054371
rosa_laevigata RLG00000034927
rosa_multiflora Rmu_sc0005705.1_g000023 Rmu_sc0023043.1_g000001
rosa_roxburghii Rroxscaffold_1G00026500
rosa_rugosa Rorug05G0285900
rosa_samantha Rh5AG356400 Rh5BG368000 Rh5CG391300 Rh5DG382200
rosa_wichuraiana Rw5G033480 Rw5G033650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 505, 1019
AccB7I CCANNNNNTGG 1 cut(s) 1345
AccI GTMKAC 2 cut(s) 797, 970
AccII CGCG 3 cut(s) 95, 118, 522
AciI CCGC 7 cut(s) 48, 95, 118, 255, 522, 917, 1091
AclWI GGATC 3 cut(s) 304, 840, 935
AcoI YGGCCR 4 cut(s) 34, 582, 605, 1336
AcsI RAATTY 1 cut(s) 983
AcuI CTGAAG 2 cut(s) 429, 1056
AcyI GRCGYC 1 cut(s) 506
AdeI CACNNNGTG 1 cut(s) 377
AfaI GTAC 7 cut(s) 185, 227, 245, 249, 568, 949, 1398
AfiI CCNNNNNNNGG 5 cut(s) 32, 136, 607, 628, 1345
AflII CTTAAG 1 cut(s) 1352
AgsI TTSAA 8 cut(s) 360, 542, 578, 857, 866, 1045, 1128, 1271
AjiI CACGTC 1 cut(s) 474
AjnI CCWGG 1 cut(s) 602
AleI CACNNNNGTG 1 cut(s) 494
AluBI AGCT 9 cut(s) 112, 160, 165, 295, 749, 757, 810, 872, 1117
AluI AGCT 9 cut(s) 112, 160, 165, 295, 749, 757, 810, 872, 1117
Alw21I GWGCWC 4 cut(s) 167, 297, 812, 1004
Alw26I GTCTC 1 cut(s) 61
Alw44I GTGCAC 1 cut(s) 1000
AlwI GGATC 3 cut(s) 304, 840, 935
Ama87I CYCGRG 2 cut(s) 296, 1471
AoxI GGCC 5 cut(s) 34, 582, 605, 838, 1336
ApaLI GTGCAC 1 cut(s) 1000
ApoI RAATTY 1 cut(s) 983
ArsI GACNNNNNNTTYG 2 cut(s) 1232, 1264
Asp700I GAANNNNTTC 1 cut(s) 351
AspLEI GCGC 1 cut(s) 508
AspS9I GGNCC 1 cut(s) 23
AsuC2I CCSGG 1 cut(s) 252
AsuHPI GGTGA 4 cut(s) 16, 95, 109, 668
AsuII TTCGAA 2 cut(s) 277, 686
AvaI CYCGRG 2 cut(s) 296, 1471
AvaII GGWCC 1 cut(s) 23
BaeGI GKGCMC 3 cut(s) 421, 559, 1004
BalI TGGCCA 2 cut(s) 584, 1338
BanI GGYRCC 2 cut(s) 505, 1019
BanII GRGCYC 3 cut(s) 167, 297, 812
BbsI GAAGAC 3 cut(s) 82, 286, 1365
Bbv12I GWGCWC 4 cut(s) 167, 297, 812, 1004
BccI CCATC 2 cut(s) 38, 1202
BceAI ACGGC 1 cut(s) 21
BcgI CGANNNNNNTGC 2 cut(s) 456, 490
BciT130I CCWGG 1 cut(s) 604
BcnI CCSGG 1 cut(s) 252
BcoDI GTCTC 1 cut(s) 61
BfaI CTAG 3 cut(s) 18, 62, 1501
BfmI CTRYAG 2 cut(s) 69, 1430
BfoI RGCGCY 1 cut(s) 509
BfrI CTTAAG 1 cut(s) 1352
BisI GCNGC 2 cut(s) 256, 1092
BlpI GCTNAGC 2 cut(s) 108, 161
BlsI GCNGC 2 cut(s) 257, 1093
BmcAI AGTACT 1 cut(s) 185
Bme1390I CCNGG 2 cut(s) 252, 604
Bme18I GGWCC 1 cut(s) 23
BmeT110I CYCGRG 2 cut(s) 296, 1471
BmgBI CACGTC 1 cut(s) 474
BmgT120I GGNCC 1 cut(s) 23
BmiI GGNNCC 3 cut(s) 43, 507, 1021
BmrFI CCNGG 2 cut(s) 252, 604
BmsI GCATC 5 cut(s) 259, 334, 476, 775, 1112
BpiI GAAGAC 3 cut(s) 82, 286, 1365
BplI GAGNNNNNCTC 4 cut(s) 154, 186, 799, 831
Bpu1102I GCTNAGC 2 cut(s) 108, 161
Bpu14I TTCGAA 2 cut(s) 277, 686
BpuEI CTTGAG 2 cut(s) 727, 782
BpuMI CCSGG 1 cut(s) 252
BsaAI YACGTR 1 cut(s) 247
BsaBI GATNNNNATC 1 cut(s) 938
BsaHI GRCGYC 1 cut(s) 506
BsaJI CCNNGG 4 cut(s) 37, 602, 674, 1023
BsaWI WCCGGW 3 cut(s) 80, 639, 653
BsaXI ACNNNNNCTCC 2 cut(s) 1203, 1233
Bsc4I CCNNNNNNNGG 5 cut(s) 32, 136, 607, 628, 1345
Bse118I RCCGGY 1 cut(s) 493
Bse1I ACTGG 2 cut(s) 712, 906
Bse3DI GCAATG 1 cut(s) 1021
Bse8I GATNNNNATC 1 cut(s) 938
BseBI CCWGG 1 cut(s) 604
BseDI CCNNGG 4 cut(s) 37, 602, 674, 1023
BseGI GGATG 2 cut(s) 467, 766
BseJI GATNNNNATC 1 cut(s) 938
BseLI CCNNNNNNNGG 5 cut(s) 32, 136, 607, 628, 1345
BseMI GCAATG 1 cut(s) 1021
BseMII CTCAG 4 cut(s) 99, 152, 797, 890
BseNI ACTGG 2 cut(s) 712, 906
BseRI GAGGAG 3 cut(s) 159, 314, 864
BseSI GKGCMC 3 cut(s) 421, 559, 1004
BseX3I CGGCCG 1 cut(s) 34
BsgI GTGCAG 1 cut(s) 356
Bsh1236I CGCG 3 cut(s) 95, 118, 522
Bsh1285I CGRYCG 2 cut(s) 37, 122
BshFI GGCC 5 cut(s) 36, 584, 607, 840, 1338
BshNI GGYRCC 2 cut(s) 505, 1019
BsiEI CGRYCG 2 cut(s) 37, 122
BsiHKAI GWGCWC 4 cut(s) 167, 297, 812, 1004
BsiHKCI CYCGRG 2 cut(s) 296, 1471
BsiSI CCGG 9 cut(s) 81, 252, 486, 494, 509, 608, 640, 654, 665
BslI CCNNNNNNNGG 5 cut(s) 32, 136, 607, 628, 1345
BsmAI GTCTC 1 cut(s) 61
BsmBI CGTCTC 1 cut(s) 61
BsnI GGCC 5 cut(s) 36, 584, 607, 840, 1338
BsoBI CYCGRG 2 cut(s) 296, 1471
Bsp119I TTCGAA 2 cut(s) 277, 686
Bsp1286I GDGCHC 6 cut(s) 167, 297, 421, 559, 812, 1004
Bsp1407I TGTACA 2 cut(s) 225, 566
Bsp143I GATC 8 cut(s) 309, 423, 436, 832, 927, 939, 1414, 1426
Bsp1720I GCTNAGC 2 cut(s) 108, 161
BspACI CCGC 7 cut(s) 48, 95, 118, 255, 522, 917, 1091
BspANI GGCC 5 cut(s) 36, 584, 607, 840, 1338
BspCNI CTCAG 4 cut(s) 100, 153, 798, 889
BspFNI CGCG 3 cut(s) 95, 118, 522
BspLI GGNNCC 3 cut(s) 43, 507, 1021
BspMAI CTGCAG 1 cut(s) 73
BspPI GGATC 3 cut(s) 304, 840, 935
BspQI GCTCTTC 1 cut(s) 522
BspT104I TTCGAA 2 cut(s) 277, 686
BspT107I GGYRCC 2 cut(s) 505, 1019
BspTI CTTAAG 1 cut(s) 1352
BsrDI GCAATG 1 cut(s) 1021
BsrFI RCCGGY 1 cut(s) 493
BsrGI TGTACA 2 cut(s) 225, 566
BsrI ACTGG 2 cut(s) 712, 906
BssAI RCCGGY 1 cut(s) 493
BssECI CCNNGG 4 cut(s) 37, 602, 674, 1023
BssMI GATC 8 cut(s) 309, 423, 436, 832, 927, 939, 1414, 1426
BssNAI GTATAC 1 cut(s) 971
BssNI GRCGYC 1 cut(s) 506
Bst1107I GTATAC 1 cut(s) 971
Bst2UI CCWGG 1 cut(s) 604
Bst4CI ACNGT 6 cut(s) 55, 1006, 1099, 1124, 1193, 1345
Bst6I CTCTTC 2 cut(s) 405, 522
BstACI GRCGYC 1 cut(s) 506
BstAFI CTTAAG 1 cut(s) 1352
BstAPI GCANNNNNTGC 1 cut(s) 1100
BstAUI TGTACA 2 cut(s) 225, 566
BstBAI YACGTR 1 cut(s) 247
BstBI TTCGAA 2 cut(s) 277, 686
BstC8I GCNNGC 3 cut(s) 260, 491, 520
BstDEI CTNAG 7 cut(s) 108, 156, 161, 750, 806, 876, 1493
BstDSI CCRYGG 1 cut(s) 37
BstENI CCTNNNNNAGG 1 cut(s) 134
BstF5I GGATG 2 cut(s) 467, 766
BstFNI CGCG 3 cut(s) 95, 118, 522
BstH2I RGCGCY 1 cut(s) 509
BstHHI GCGC 1 cut(s) 508
BstKTI GATC 8 cut(s) 312, 426, 439, 835, 930, 942, 1417, 1429
BstMAI GTCTC 1 cut(s) 61
BstMBI GATC 8 cut(s) 309, 423, 436, 832, 927, 939, 1414, 1426
BstMCI CGRYCG 2 cut(s) 37, 122
BstMWI GCNNNNNNNGC 3 cut(s) 139, 763, 1100
BstNI CCWGG 1 cut(s) 604
BstNSI RCATGY 1 cut(s) 729
BstSCI CCNGG 2 cut(s) 250, 602
BstSFI CTRYAG 2 cut(s) 69, 1430
BstSLI GKGCMC 3 cut(s) 421, 559, 1004
BstSNI TACGTA 1 cut(s) 247
BstUI CGCG 3 cut(s) 95, 118, 522
BstV2I GAAGAC 3 cut(s) 82, 286, 1365
BstZ17I GTATAC 1 cut(s) 971
BstZI CGGCCG 1 cut(s) 34
BsuRI GGCC 5 cut(s) 36, 584, 607, 840, 1338
BtgI CCRYGG 1 cut(s) 37
BtrI CACGTC 1 cut(s) 474
BtsCI GGATG 2 cut(s) 467, 766
BtsI GCAGTG 2 cut(s) 66, 149
BtsIMutI CAGTG 5 cut(s) 66, 149, 204, 719, 1002
Cac8I GCNNGC 3 cut(s) 260, 491, 520
CfoI GCGC 1 cut(s) 508
Cfr10I RCCGGY 1 cut(s) 493
Cfr13I GGNCC 1 cut(s) 23
Csp6I GTAC 7 cut(s) 184, 226, 244, 248, 567, 948, 1397
CviAII CATG 7 cut(s) 173, 238, 284, 461, 502, 726, 1408
CviQI GTAC 7 cut(s) 184, 226, 244, 248, 567, 948, 1397
DdeI CTNAG 7 cut(s) 108, 156, 161, 750, 806, 876, 1493
DinI GGCGCC 1 cut(s) 507
DpnI GATC 8 cut(s) 311, 425, 438, 834, 929, 941, 1416, 1428
DpnII GATC 8 cut(s) 309, 423, 436, 832, 927, 939, 1414, 1426
DraIII CACNNNGTG 1 cut(s) 377
EaeI YGGCCR 4 cut(s) 34, 582, 605, 1336
EagI CGGCCG 1 cut(s) 34
Eam1104I CTCTTC 2 cut(s) 405, 522
EarI CTCTTC 2 cut(s) 405, 522
Ecl136II GAGCTC 3 cut(s) 165, 295, 810
EclXI CGGCCG 1 cut(s) 34
Eco105I TACGTA 1 cut(s) 247
Eco147I AGGCCT 1 cut(s) 840
Eco24I GRGCYC 3 cut(s) 167, 297, 812
Eco47I GGWCC 1 cut(s) 23
Eco52I CGGCCG 1 cut(s) 34
Eco53kI GAGCTC 3 cut(s) 165, 295, 810
Eco57I CTGAAG 2 cut(s) 429, 1056
Eco88I CYCGRG 2 cut(s) 296, 1471
EcoICRI GAGCTC 3 cut(s) 165, 295, 810
EcoNI CCTNNNNNAGG 1 cut(s) 134
EcoRII CCWGG 1 cut(s) 602
EcoT22I ATGCAT 1 cut(s) 1445
EcoT38I GRGCYC 3 cut(s) 167, 297, 812
EgeI GGCGCC 1 cut(s) 507
EheI GGCGCC 1 cut(s) 507
Esp3I CGTCTC 1 cut(s) 61
FaeI CATG 7 cut(s) 176, 241, 287, 464, 505, 729, 1411
FalI AAGNNNNNCTT 4 cut(s) 689, 721, 1346, 1378
FatI CATG 7 cut(s) 172, 237, 283, 460, 501, 725, 1407
FblI GTMKAC 2 cut(s) 797, 970
Fnu4HI GCNGC 2 cut(s) 256, 1092
FokI GGATG 2 cut(s) 454, 753
FriOI GRGCYC 3 cut(s) 167, 297, 812
Fsp4HI GCNGC 2 cut(s) 256, 1092
FspBI CTAG 3 cut(s) 18, 62, 1501
GlaI GCGC 1 cut(s) 507
GluI GCNGC 2 cut(s) 256, 1092
HaeII RGCGCY 1 cut(s) 509
HaeIII GGCC 5 cut(s) 36, 584, 607, 840, 1338
HapII CCGG 9 cut(s) 81, 252, 486, 494, 509, 608, 640, 654, 665
HhaI GCGC 1 cut(s) 508
Hin1I GRCGYC 1 cut(s) 506
Hin1II CATG 7 cut(s) 176, 241, 287, 464, 505, 729, 1411
Hin6I GCGC 1 cut(s) 506
HinP1I GCGC 1 cut(s) 506
HindIII AAGCTT 2 cut(s) 755, 1115
HinfI GANTC 8 cut(s) 274, 648, 802, 848, 880, 1166, 1184, 1244
HpaII CCGG 9 cut(s) 81, 252, 486, 494, 509, 608, 640, 654, 665
HphI GGTGA 4 cut(s) 16, 95, 109, 668
Hpy166II GTNNAC 6 cut(s) 15, 744, 798, 971, 1002, 1255
Hpy188III TCNNGA 5 cut(s) 706, 761, 931, 1262, 1424
Hpy8I GTNNAC 6 cut(s) 15, 744, 798, 971, 1002, 1255
Hpy99I CGWCG 2 cut(s) 127, 478
HpyAV CCTTC 1 cut(s) 1447
HpyCH4III ACNGT 6 cut(s) 55, 1006, 1099, 1124, 1193, 1345
HpyCH4IV ACGT 2 cut(s) 246, 473
HpyCH4V TGCA 6 cut(s) 71, 337, 460, 1002, 1103, 1443
HpyF10VI GCNNNNNNNGC 3 cut(s) 139, 763, 1100
HpyF3I CTNAG 7 cut(s) 108, 156, 161, 750, 806, 876, 1493
HpySE526I ACGT 2 cut(s) 246, 473
Hsp92I GRCGYC 1 cut(s) 506
Hsp92II CATG 7 cut(s) 176, 241, 287, 464, 505, 729, 1411
HspAI GCGC 1 cut(s) 506
KasI GGCGCC 1 cut(s) 505
Kzo9I GATC 8 cut(s) 309, 423, 436, 832, 927, 939, 1414, 1426
LguI GCTCTTC 1 cut(s) 522
LmnI GCTCC 4 cut(s) 47, 139, 292, 877
LweI GCATC 5 cut(s) 259, 334, 476, 775, 1112
MaeI CTAG 3 cut(s) 18, 62, 1501
MaeII ACGT 2 cut(s) 246, 473
MaeIII GTNAC 4 cut(s) 377, 617, 656, 1234
MalI GATC 8 cut(s) 311, 425, 438, 834, 929, 941, 1416, 1428
MboI GATC 8 cut(s) 309, 423, 436, 832, 927, 939, 1414, 1426
MfeI CAATTG 2 cut(s) 360, 976
MhlI GDGCHC 6 cut(s) 167, 297, 421, 559, 812, 1004
MlsI TGGCCA 2 cut(s) 584, 1338
MluNI TGGCCA 2 cut(s) 584, 1338
Mly113I GGCGCC 1 cut(s) 506
MlyI GAGTC 2 cut(s) 796, 1193
MmeI TCCRAC 1 cut(s) 810
Mox20I TGGCCA 2 cut(s) 584, 1338
Mph1103I ATGCAT 1 cut(s) 1445
MroXI GAANNNNTTC 1 cut(s) 351
MscI TGGCCA 2 cut(s) 584, 1338
MseI TTAA 4 cut(s) 384, 777, 1353, 1367
MslI CAYNNNNRTG 3 cut(s) 401, 494, 1448
Msp20I TGGCCA 2 cut(s) 584, 1338
MspCI CTTAAG 1 cut(s) 1352
MspI CCGG 9 cut(s) 81, 252, 486, 494, 509, 608, 640, 654, 665
MspR9I CCNGG 2 cut(s) 252, 604
MunI CAATTG 2 cut(s) 360, 976
MvaI CCWGG 1 cut(s) 604
MvnI CGCG 3 cut(s) 95, 118, 522
MwoI GCNNNNNNNGC 3 cut(s) 139, 763, 1100
NarI GGCGCC 1 cut(s) 506
NciI CCSGG 1 cut(s) 252
NdeII GATC 8 cut(s) 309, 423, 436, 832, 927, 939, 1414, 1426
NlaIII CATG 7 cut(s) 176, 241, 287, 464, 505, 729, 1411
NlaIV GGNNCC 3 cut(s) 43, 507, 1021
NmeAIII GCCGAG 1 cut(s) 1048
NmuCI GTSAC 3 cut(s) 377, 617, 656
NsiI ATGCAT 1 cut(s) 1445
NspI RCATGY 1 cut(s) 729
NspV TTCGAA 2 cut(s) 277, 686
OliI CACNNNNGTG 1 cut(s) 494
PaeR7I CTCGAG 2 cut(s) 296, 1471
PceI AGGCCT 1 cut(s) 840
PciSI GCTCTTC 1 cut(s) 522
PcsI WCGNNNNNNNCGW 2 cut(s) 122, 1248
PdmI GAANNNNTTC 1 cut(s) 351
PfeI GAWTC 6 cut(s) 274, 648, 848, 880, 1166, 1244
PflMI CCANNNNNTGG 1 cut(s) 1345
PkrI GCNGC 2 cut(s) 257, 1093
PleI GAGTC 2 cut(s) 796, 1192
PluTI GGCGCC 1 cut(s) 509
PpsI GAGTC 2 cut(s) 796, 1192
Ppu21I YACGTR 1 cut(s) 247
Psp124BI GAGCTC 3 cut(s) 167, 297, 812
Psp6I CCWGG 1 cut(s) 602
PspGI CCWGG 1 cut(s) 602
PspN4I GGNNCC 3 cut(s) 43, 507, 1021
PspPI GGNCC 1 cut(s) 23
PspXI VCTCGAGB 2 cut(s) 296, 1471
PstI CTGCAG 1 cut(s) 73
RsaI GTAC 7 cut(s) 185, 227, 245, 249, 568, 949, 1398
RsaNI GTAC 7 cut(s) 184, 226, 244, 248, 567, 948, 1397
RseI CAYNNNNRTG 3 cut(s) 401, 494, 1448
SacI GAGCTC 3 cut(s) 167, 297, 812
SapI GCTCTTC 1 cut(s) 522
SaqAI TTAA 4 cut(s) 384, 777, 1353, 1367
SatI GCNGC 2 cut(s) 256, 1092
Sau3AI GATC 8 cut(s) 309, 423, 436, 832, 927, 939, 1414, 1426
Sau96I GGNCC 1 cut(s) 23
ScaI AGTACT 1 cut(s) 185
SchI GAGTC 2 cut(s) 796, 1193
ScrFI CCNGG 2 cut(s) 252, 604
SduI GDGCHC 6 cut(s) 167, 297, 421, 559, 812, 1004
SfaNI GCATC 5 cut(s) 259, 334, 476, 775, 1112
SfcI CTRYAG 2 cut(s) 69, 1430
SfoI GGCGCC 1 cut(s) 507
Sfr274I CTCGAG 2 cut(s) 296, 1471
SfuI TTCGAA 2 cut(s) 277, 686
SgrAI CRCCGGYG 1 cut(s) 493
SinI GGWCC 1 cut(s) 23
SlaI CTCGAG 2 cut(s) 296, 1471
SmiMI CAYNNNNRTG 3 cut(s) 401, 494, 1448
SmlI CTYRAG 5 cut(s) 296, 706, 761, 1352, 1471
SmoI CTYRAG 5 cut(s) 296, 706, 761, 1352, 1471
SnaBI TACGTA 1 cut(s) 247
SseBI AGGCCT 1 cut(s) 840
SsiI CCGC 7 cut(s) 48, 95, 118, 255, 522, 917, 1091
SspDI GGCGCC 1 cut(s) 505
SspMI CTAG 3 cut(s) 18, 62, 1501
SstI GAGCTC 3 cut(s) 167, 297, 812
StuI AGGCCT 1 cut(s) 840
StyD4I CCNGG 2 cut(s) 250, 602
TaaI ACNGT 6 cut(s) 55, 1006, 1099, 1124, 1193, 1345
TaiI ACGT 2 cut(s) 249, 476
TaqI TCGA 5 cut(s) 277, 297, 686, 1425, 1472
TatI WGTACW 3 cut(s) 183, 225, 566
TauI GCSGC 2 cut(s) 258, 1094
TfiI GAWTC 6 cut(s) 274, 648, 848, 880, 1166, 1244
Tru1I TTAA 4 cut(s) 384, 777, 1353, 1367
Tru9I TTAA 4 cut(s) 384, 777, 1353, 1367
TscAI CASTG 5 cut(s) 73, 149, 211, 719, 1009
TseFI GTSAC 3 cut(s) 377, 617, 656
Tsp45I GTSAC 3 cut(s) 377, 617, 656
TspDTI ATGAA 7 cut(s) 226, 344, 477, 490, 840, 1155, 1215
TspRI CASTG 5 cut(s) 73, 149, 211, 719, 1009
Van91I CCANNNNNTGG 1 cut(s) 1345
Vha464I CTTAAG 1 cut(s) 1352
VneI GTGCAC 1 cut(s) 1000
VpaK11BI GGWCC 1 cut(s) 23
XagI CCTNNNNNAGG 1 cut(s) 134
XapI RAATTY 1 cut(s) 983
XceI RCATGY 1 cut(s) 729
XcmI CCANNNNNNNNNTGG 1 cut(s) 738
XhoI CTCGAG 2 cut(s) 296, 1471
XmiI GTMKAC 2 cut(s) 797, 970
XmnI GAANNNNTTC 1 cut(s) 351
XspI CTAG 3 cut(s) 18, 62, 1501
ZrmI AGTACT 1 cut(s) 185
Zsp2I ATGCAT 1 cut(s) 1445
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.