Rh5CG391300

Pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0013350
Physical Location & Seq
Reverse (-)
51526896 .. 51529555
2660 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG391300.1

Sequence Viewer

Length: 1023 bp
ATGGAATTGGGCGTTTTTAGTGCTTCTGTTGCTGGTTTAGTACTGCTGGCTTTGTTGGGTGGTTTTCAGTTGGGTTTCTCACAAAGTGAAGCATCTGCAAGGAGTTATATTAGCTGGGATGATTTGAAGGTGGATGAGCAGAAGGTTATTGATTTGAACTCTAGAAATGAAGAGCAAAACGGGACGCGCATCATTGTGGTGGACAAGAATGGAGAAGGAAACTCTGTGACAGTTCAAGGAGCAGTTGACATGGTTCCACACCATAACACCCAAAGAGTCAAAATATACTTTCTTCCAGGAATTTATAGAGAGAAGGTGCTTATACCAATTACCAAGCCATACATATCATTTATAGGGGACCAGAATCGAACTTCTGAGACTGTAATTACTTGGAACAGTAAGGCATCTGATAAGGACAGCAATGGCTTTGAACTAGGAACGTACAGAACAGCAACTGTAGCCATAGAGGCTGATTACTTTTGTGCAACAGGGATCACTTTCGAGAACAGTGCGGTTGCAGTGCCTGGAGGATATGGAATGCAAGGAGTGGCTCTTAGAGTTGCTGGGGATAAGGCCATGTTCTATAGAGTAAGGGTTTTGGGAGCACAAGATACATTATTGGATGAGAATGGATCGCACTACTTCTACGAATCCCACATTCAAGGAAGTGTGGACTTCATATTTGGAAAATCAAGGTCACTCTACCAGGACTGTGTTATCCAATCAACTGCTAAGACCTATGGAGCAATTGCAGCTCATCATAGAGACTCACCAAATGAAGATACGGGGTTCTCTTTCGTGAACTGCAAAATCATCGGAACTGGAGATATCTACTTGGGAAGAGCTTGGGGAGCTTATTCAAGAGTAATATATTCCTATTGTCATATTGACAATATAATAACTCCTCTGGGATGGAATGACTGGAACATGCCACCAAGGCAGAGGTATTCTTCAAGCTTGAACATTAGGCATCTTGAATTATCTCCAAGCAGAGCACTCAAATCACATTATTGCTCCCTTTAA

Protein Analysis

340

Amino Acids

37.69

Weight (kDa)

6.14

Isoelectric Point (pI)

32.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 65 - 310 1.3e-55 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013350)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G55590 AT5G55590
fragaria_vesca FvH4_3g29490
malus_domestica MD03G1186900.v1.1 MD11G1201900.v1.1
prunus_persica Prupe.4G239700_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0054371
rosa_laevigata RLG00000034927
rosa_multiflora Rmu_sc0005705.1_g000023 Rmu_sc0023043.1_g000001
rosa_roxburghii Rroxscaffold_1G00026500
rosa_rugosa Rorug05G0285900
rosa_samantha Rh5AG356400 Rh5BG368000 Rh5CG391300 Rh5DG382200
rosa_wichuraiana Rw5G033480 Rw5G033650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 187
AciI CCGC 1 cut(s) 512
AclWI GGATC 2 cut(s) 500, 640
AcsI RAATTY 1 cut(s) 300
AdeI CACNNNGTG 1 cut(s) 86
AfaI GTAC 2 cut(s) 42, 443
AgsI TTSAA 9 cut(s) 127, 157, 236, 431, 662, 861, 954, 961, 977
AjnI CCWGG 3 cut(s) 295, 523, 705
AluBI AGCT 5 cut(s) 114, 755, 845, 854, 957
AluI AGCT 5 cut(s) 114, 755, 845, 854, 957
Alw21I GWGCWC 2 cut(s) 607, 997
Alw26I GTCTC 2 cut(s) 371, 759
AlwI GGATC 2 cut(s) 500, 640
AlwNI CAGNNNCTG 2 cut(s) 455, 524
AoxI GGCC 1 cut(s) 573
ApeKI GCWGC 1 cut(s) 752
ApoI RAATTY 1 cut(s) 300
ArsI GACNNNNNNTTYG 2 cut(s) 665, 697
AspLEI GCGC 1 cut(s) 189
AspS9I GGNCC 1 cut(s) 358
AsuHPI GGTGA 1 cut(s) 762
AvaII GGWCC 1 cut(s) 358
Bbv12I GWGCWC 2 cut(s) 607, 997
BbvI GCAGC 1 cut(s) 764
BccI CCATC 1 cut(s) 906
BcgI CGANNNNNNTGC 4 cut(s) 491, 525, 796, 830
BciT130I CCWGG 3 cut(s) 297, 525, 707
BcoDI GTCTC 2 cut(s) 371, 759
BfaI CTAG 2 cut(s) 162, 434
BfmI CTRYAG 2 cut(s) 456, 583
BglI GCCNNNNNGGC 2 cut(s) 467, 937
BisI GCNGC 1 cut(s) 753
BlsI GCNGC 1 cut(s) 754
BmcAI AGTACT 1 cut(s) 42
Bme1390I CCNGG 3 cut(s) 297, 525, 707
Bme18I GGWCC 1 cut(s) 358
BmgT120I GGNCC 1 cut(s) 358
BmiI GGNNCC 2 cut(s) 255, 359
BmrFI CCNGG 3 cut(s) 297, 525, 707
BmsI GCATC 4 cut(s) 101, 198, 413, 979
BpmI CTGGAG 2 cut(s) 546, 843
BsaJI CCNNGG 1 cut(s) 935
Bse1I ACTGG 2 cut(s) 826, 926
Bse3DI GCAATG 1 cut(s) 427
BseBI CCWGG 3 cut(s) 297, 525, 707
BseDI CCNNGG 1 cut(s) 935
BseGI GGATG 4 cut(s) 124, 139, 628, 917
BseMI GCAATG 1 cut(s) 427
BseMII CTCAG 1 cut(s) 366
BseNI ACTGG 2 cut(s) 826, 926
BseRI GAGGAG 1 cut(s) 894
BseXI GCAGC 1 cut(s) 764
BseYI CCCAGC 2 cut(s) 114, 563
Bsh1236I CGCG 1 cut(s) 187
BshFI GGCC 1 cut(s) 575
BsiHKAI GWGCWC 2 cut(s) 607, 997
BslFI GGGAC 2 cut(s) 196, 371
BsmAI GTCTC 2 cut(s) 371, 759
BsmFI GGGAC 2 cut(s) 196, 371
BsmI GAATGC 1 cut(s) 543
BsnI GGCC 1 cut(s) 575
Bsp1286I GDGCHC 2 cut(s) 607, 997
Bsp143I GATC 2 cut(s) 492, 632
BspACI CCGC 1 cut(s) 512
BspANI GGCC 1 cut(s) 575
BspCNI CTCAG 1 cut(s) 367
BspFNI CGCG 1 cut(s) 187
BspLI GGNNCC 2 cut(s) 255, 359
BspPI GGATC 2 cut(s) 500, 640
BspQI GCTCTTC 2 cut(s) 165, 835
BsrDI GCAATG 1 cut(s) 427
BsrI ACTGG 2 cut(s) 826, 926
BssECI CCNNGG 1 cut(s) 935
BssMI GATC 2 cut(s) 492, 632
BssT1I CCWWGG 1 cut(s) 935
Bst2UI CCWGG 3 cut(s) 297, 525, 707
Bst4CI ACNGT 6 cut(s) 232, 382, 398, 457, 509, 713
Bst6I CTCTTC 2 cut(s) 165, 835
BstC8I GCNNGC 1 cut(s) 48
BstDEI CTNAG 3 cut(s) 375, 554, 732
BstF5I GGATG 4 cut(s) 124, 139, 628, 917
BstFNI CGCG 1 cut(s) 187
BstHHI GCGC 1 cut(s) 189
BstKTI GATC 2 cut(s) 495, 635
BstMAI GTCTC 2 cut(s) 371, 759
BstMBI GATC 2 cut(s) 492, 632
BstMWI GCNNNNNNNGC 6 cut(s) 29, 458, 467, 752, 851, 937
BstNI CCWGG 3 cut(s) 297, 525, 707
BstNSI RCATGY 1 cut(s) 931
BstSCI CCNGG 3 cut(s) 295, 523, 705
BstSFI CTRYAG 2 cut(s) 456, 583
BstUI CGCG 1 cut(s) 187
BstV1I GCAGC 1 cut(s) 764
BsuRI GGCC 1 cut(s) 575
BtsCI GGATG 4 cut(s) 124, 139, 628, 917
BtsI GCAGTG 1 cut(s) 525
BtsIMutI CAGTG 2 cut(s) 514, 525
Cac8I GCNNGC 1 cut(s) 48
CaiI CAGNNNCTG 2 cut(s) 455, 524
CfoI GCGC 1 cut(s) 189
Cfr13I GGNCC 1 cut(s) 358
CseI GACGC 1 cut(s) 193
Csp6I GTAC 2 cut(s) 41, 442
CviAII CATG 3 cut(s) 250, 577, 928
CviQI GTAC 2 cut(s) 41, 442
DdeI CTNAG 3 cut(s) 375, 554, 732
DpnI GATC 2 cut(s) 494, 634
DpnII GATC 2 cut(s) 492, 632
DraIII CACNNNGTG 1 cut(s) 86
Eam1104I CTCTTC 2 cut(s) 165, 835
EarI CTCTTC 2 cut(s) 165, 835
Eco130I CCWWGG 1 cut(s) 935
Eco32I GATATC 1 cut(s) 829
Eco47I GGWCC 1 cut(s) 358
EcoRII CCWGG 3 cut(s) 295, 523, 705
EcoRV GATATC 1 cut(s) 829
EcoT14I CCWWGG 1 cut(s) 935
ErhI CCWWGG 1 cut(s) 935
FaeI CATG 3 cut(s) 253, 580, 931
FaqI GGGAC 2 cut(s) 196, 371
FatI CATG 3 cut(s) 249, 576, 927
Fnu4HI GCNGC 1 cut(s) 753
FokI GGATG 4 cut(s) 131, 146, 635, 924
Fsp4HI GCNGC 1 cut(s) 753
FspBI CTAG 2 cut(s) 162, 434
GlaI GCGC 1 cut(s) 188
GluI GCNGC 1 cut(s) 753
GsaI CCCAGC 2 cut(s) 118, 567
GsuI CTGGAG 2 cut(s) 546, 843
HaeIII GGCC 1 cut(s) 575
HgaI GACGC 1 cut(s) 193
HhaI GCGC 1 cut(s) 189
Hin1II CATG 3 cut(s) 253, 580, 931
Hin6I GCGC 1 cut(s) 187
HinP1I GCGC 1 cut(s) 187
HincII GTYRAC 1 cut(s) 247
HindII GTYRAC 1 cut(s) 247
HindIII AAGCTT 1 cut(s) 955
HinfI GANTC 4 cut(s) 276, 364, 650, 767
HphI GGTGA 1 cut(s) 762
Hpy166II GTNNAC 4 cut(s) 202, 247, 673, 802
Hpy188I TCNGA 3 cut(s) 376, 409, 818
Hpy188III TCNNGA 5 cut(s) 162, 502, 799, 861, 974
Hpy8I GTNNAC 4 cut(s) 202, 247, 673, 802
HpyAV CCTTC 4 cut(s) 121, 136, 209, 307
HpyCH4III ACNGT 6 cut(s) 232, 382, 398, 457, 509, 713
HpyCH4IV ACGT 1 cut(s) 440
HpyCH4V TGCA 6 cut(s) 98, 485, 518, 541, 752, 807
HpyF10VI GCNNNNNNNGC 6 cut(s) 29, 458, 467, 752, 851, 937
HpyF3I CTNAG 3 cut(s) 375, 554, 732
HpySE526I ACGT 1 cut(s) 440
Hsp92II CATG 3 cut(s) 253, 580, 931
HspAI GCGC 1 cut(s) 187
Kzo9I GATC 2 cut(s) 492, 632
LguI GCTCTTC 2 cut(s) 165, 835
LmnI GCTCC 5 cut(s) 239, 602, 743, 851, 1019
Lsp1109I GCAGC 1 cut(s) 764
LweI GCATC 4 cut(s) 101, 198, 413, 979
MaeI CTAG 2 cut(s) 162, 434
MaeII ACGT 1 cut(s) 440
MaeIII GTNAC 2 cut(s) 226, 696
MalI GATC 2 cut(s) 494, 634
MboI GATC 2 cut(s) 492, 632
MboII GAAGA 5 cut(s) 182, 284, 791, 852, 942
MfeI CAATTG 1 cut(s) 747
MhlI GDGCHC 2 cut(s) 607, 997
MluCI AATT 6 cut(s) 5, 300, 327, 384, 747, 977
MlyI GAGTC 2 cut(s) 285, 761
MnlI CCTC 4 cut(s) 460, 521, 915, 936
MseI TTAA 1 cut(s) 1021
MslI CAYNNNNRTG 2 cut(s) 194, 197
MspR9I CCNGG 3 cut(s) 297, 525, 707
MunI CAATTG 1 cut(s) 747
Mva1269I GAATGC 1 cut(s) 543
MvaI CCWGG 3 cut(s) 297, 525, 707
MvnI CGCG 1 cut(s) 187
MwoI GCNNNNNNNGC 6 cut(s) 29, 458, 467, 752, 851, 937
NdeII GATC 2 cut(s) 492, 632
NlaIII CATG 3 cut(s) 253, 580, 931
NlaIV GGNNCC 2 cut(s) 255, 359
NmuCI GTSAC 2 cut(s) 226, 696
NspI RCATGY 1 cut(s) 931
PciSI GCTCTTC 2 cut(s) 165, 835
PctI GAATGC 1 cut(s) 543
PfeI GAWTC 2 cut(s) 364, 650
PfoI TCCNGGA 1 cut(s) 295
PkrI GCNGC 1 cut(s) 754
PleI GAGTC 2 cut(s) 284, 761
PpsI GAGTC 2 cut(s) 284, 761
Psp6I CCWGG 3 cut(s) 295, 523, 705
PspFI CCCAGC 2 cut(s) 114, 563
PspGI CCWGG 3 cut(s) 295, 523, 705
PspN4I GGNNCC 2 cut(s) 255, 359
PspPI GGNCC 1 cut(s) 358
PstNI CAGNNNCTG 2 cut(s) 455, 524
RsaI GTAC 2 cut(s) 42, 443
RsaNI GTAC 2 cut(s) 41, 442
RseI CAYNNNNRTG 2 cut(s) 194, 197
SapI GCTCTTC 2 cut(s) 165, 835
SaqAI TTAA 1 cut(s) 1021
SatI GCNGC 1 cut(s) 753
Sau3AI GATC 2 cut(s) 492, 632
Sau96I GGNCC 1 cut(s) 358
ScaI AGTACT 1 cut(s) 42
SchI GAGTC 2 cut(s) 285, 761
ScrFI CCNGG 3 cut(s) 297, 525, 707
SduI GDGCHC 2 cut(s) 607, 997
SfaNI GCATC 4 cut(s) 101, 198, 413, 979
SfcI CTRYAG 2 cut(s) 456, 583
SinI GGWCC 1 cut(s) 358
SmiMI CAYNNNNRTG 2 cut(s) 194, 197
Sse9I AATT 6 cut(s) 5, 300, 327, 384, 747, 977
SsiI CCGC 1 cut(s) 512
SspMI CTAG 2 cut(s) 162, 434
StyD4I CCNGG 3 cut(s) 295, 523, 705
StyI CCWWGG 1 cut(s) 935
TaaI ACNGT 6 cut(s) 232, 382, 398, 457, 509, 713
TaiI ACGT 1 cut(s) 443
TaqI TCGA 2 cut(s) 367, 501
TasI AATT 6 cut(s) 5, 300, 327, 384, 747, 977
TatI WGTACW 1 cut(s) 40
TfiI GAWTC 2 cut(s) 364, 650
Tru1I TTAA 1 cut(s) 1021
Tru9I TTAA 1 cut(s) 1021
TscAI CASTG 2 cut(s) 514, 525
TseFI GTSAC 2 cut(s) 226, 696
TseI GCWGC 1 cut(s) 752
Tsp45I GTSAC 2 cut(s) 226, 696
TspDTI ATGAA 3 cut(s) 183, 667, 792
TspRI CASTG 2 cut(s) 514, 525
VpaK11BI GGWCC 1 cut(s) 358
XapI RAATTY 1 cut(s) 300
XbaI TCTAGA 1 cut(s) 161
XceI RCATGY 1 cut(s) 931
XspI CTAG 2 cut(s) 162, 434
ZrmI AGTACT 1 cut(s) 42
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.