Rh5DG382200

Pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0013350
Physical Location & Seq
Reverse (-)
58455403 .. 58458038
2636 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG382200.1

Sequence Viewer

Length: 1032 bp
ATGGAGTTGGGTGTTTTTAGTGCTTCTGTTGCTGCTGGTGCTGGTTTAGTACTACTGGTTTTGTTGGGTGGTTTTCAGTTGGGTTTCTCACAAAGTGAAGCATATGCAAGGAGTTATATCAGCTGGGATGATTTGAAGGTGGATGAGCAGATGGTCATTGGTTTGAACTCTGGAAATGAAGAGCAAAACGGGACGCGCGTCATTGTGGTGGACAAGAATGGAGAAGGAAACTCTATGACAATTCAAGCAGCAGTTGACATGGTTCCACACCATAACACCCAAAGAGTCAAAATTTACATTCTTCCAGGAATTTATAGAGAGAAGGTGCTTATACCAATTACCAAGCCATACATATCATTTATAGGGGACCAGAATCGAACTTCTGAGACTGTAATTACTTGGAACAGTAAGGCATCTGATAAGGACAGCAGTGGCTTTGAACTAGGAACGTACAGAACAGCAACTGTAGCCATAGAGGCTGATTACTTTTGTGCAACAGGGATCACTTTCGAGAACAGTGCGGTTGCAGTGCCTGGAGGATATGGAATGCAAGGAGTGGCTCTTAGAGTTGCTGGGGATAAGGCCATGTTCTATAGAGTAAGGGTTTTGGGAGCACAAGATACATTATTGGATGAGAATGGATCGCACTACTTCTACGAATCCCACATTCAAGGAAGTGTGGACTTCATATTTGGAAAATCAAGGTCACTCTACCAGGACTGTGTTATCCAATCAAGTGCTAAGACCTACGGAGCAATTGCAGCTCATCATAGAGACTCACCAAATGAAGATACAGGGTTCTCTTTCGTGAACTGCAAAATCATCGGAACTGGAGATATCTACTTGGGAAGAGCTTGGGGAGCTTATTCAAGAGTCATATATTCCTTTTGTCATATTGACAATATAATAACTCCTCTGGGATGGAGTGACTGGAACATGCCACCGAGGCAGAGGTATCCTTCAAGCTTGAACATTAGGCATCTTGAATTATCTCCAAGCAGAGCACTCAAATCACATTATTGCTCCCTCTAA

Protein Analysis

343

Amino Acids

37.78

Weight (kDa)

5.99

Isoelectric Point (pI)

34.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 68 - 313 5.7e-57 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013350)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G55590 AT5G55590
fragaria_vesca FvH4_3g29490
malus_domestica MD03G1186900.v1.1 MD11G1201900.v1.1
prunus_persica Prupe.4G239700_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0054371
rosa_laevigata RLG00000034927
rosa_multiflora Rmu_sc0005705.1_g000023 Rmu_sc0023043.1_g000001
rosa_roxburghii Rroxscaffold_1G00026500
rosa_rugosa Rorug05G0285900
rosa_samantha Rh5AG356400 Rh5BG368000 Rh5CG391300 Rh5DG382200
rosa_wichuraiana Rw5G033480 Rw5G033650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 196, 198
AciI CCGC 1 cut(s) 521
AclWI GGATC 2 cut(s) 509, 649
AcsI RAATTY 2 cut(s) 291, 309
AdeI CACNNNGTG 1 cut(s) 95
AfaI GTAC 2 cut(s) 51, 452
AgsI TTSAA 9 cut(s) 136, 166, 245, 440, 671, 870, 963, 970, 986
AjnI CCWGG 3 cut(s) 304, 532, 714
AluBI AGCT 5 cut(s) 123, 764, 854, 863, 966
AluI AGCT 5 cut(s) 123, 764, 854, 863, 966
Alw21I GWGCWC 2 cut(s) 616, 1006
Alw26I GTCTC 2 cut(s) 380, 768
AlwI GGATC 2 cut(s) 509, 649
AlwNI CAGNNNCTG 2 cut(s) 464, 533
AoxI GGCC 1 cut(s) 582
ApeKI GCWGC 3 cut(s) 32, 248, 761
ApoI RAATTY 2 cut(s) 291, 309
ArsI GACNNNNNNTTYG 2 cut(s) 674, 706
AspLEI GCGC 1 cut(s) 198
AspS9I GGNCC 1 cut(s) 367
AsuHPI GGTGA 1 cut(s) 771
AvaII GGWCC 1 cut(s) 367
Bbv12I GWGCWC 2 cut(s) 616, 1006
BbvI GCAGC 3 cut(s) 19, 260, 773
BccI CCATC 2 cut(s) 145, 915
BcgI CGANNNNNNTGC 4 cut(s) 500, 534, 805, 839
BciT130I CCWGG 3 cut(s) 306, 534, 716
BciVI GTATCC 1 cut(s) 966
BcoDI GTCTC 2 cut(s) 380, 768
BfaI CTAG 1 cut(s) 443
BfmI CTRYAG 2 cut(s) 465, 592
BfuI GTATCC 1 cut(s) 966
BglI GCCNNNNNGGC 2 cut(s) 476, 946
BisI GCNGC 3 cut(s) 33, 249, 762
BlsI GCNGC 3 cut(s) 34, 250, 763
BmcAI AGTACT 1 cut(s) 51
Bme1390I CCNGG 3 cut(s) 306, 534, 716
Bme18I GGWCC 1 cut(s) 367
BmgT120I GGNCC 1 cut(s) 367
BmiI GGNNCC 2 cut(s) 264, 368
BmrFI CCNGG 3 cut(s) 306, 534, 716
BmsI GCATC 2 cut(s) 422, 988
BoxI GACNNNNGTC 1 cut(s) 197
BpmI CTGGAG 2 cut(s) 555, 852
BsaJI CCNNGG 1 cut(s) 944
Bse1I ACTGG 3 cut(s) 60, 835, 935
BseBI CCWGG 3 cut(s) 306, 534, 716
BseDI CCNNGG 1 cut(s) 944
BseGI GGATG 4 cut(s) 133, 148, 637, 926
BseMII CTCAG 1 cut(s) 375
BseNI ACTGG 3 cut(s) 60, 835, 935
BseRI GAGGAG 1 cut(s) 903
BseXI GCAGC 3 cut(s) 19, 260, 773
BseYI CCCAGC 2 cut(s) 123, 572
Bsh1236I CGCG 2 cut(s) 196, 198
BshFI GGCC 1 cut(s) 584
BsiHKAI GWGCWC 2 cut(s) 616, 1006
BslFI GGGAC 2 cut(s) 205, 380
BsmAI GTCTC 2 cut(s) 380, 768
BsmFI GGGAC 2 cut(s) 205, 380
BsmI GAATGC 1 cut(s) 552
BsnI GGCC 1 cut(s) 584
Bsp1286I GDGCHC 2 cut(s) 616, 1006
Bsp143I GATC 2 cut(s) 501, 641
BspACI CCGC 1 cut(s) 521
BspANI GGCC 1 cut(s) 584
BspCNI CTCAG 1 cut(s) 376
BspFNI CGCG 2 cut(s) 196, 198
BspLI GGNNCC 2 cut(s) 264, 368
BspPI GGATC 2 cut(s) 509, 649
BspQI GCTCTTC 2 cut(s) 174, 844
BsrI ACTGG 3 cut(s) 60, 835, 935
BssECI CCNNGG 1 cut(s) 944
BssMI GATC 2 cut(s) 501, 641
Bst2UI CCWGG 3 cut(s) 306, 534, 716
Bst4CI ACNGT 5 cut(s) 391, 407, 466, 518, 722
Bst6I CTCTTC 2 cut(s) 174, 844
BstDEI CTNAG 3 cut(s) 384, 563, 741
BstF5I GGATG 4 cut(s) 133, 148, 637, 926
BstFNI CGCG 2 cut(s) 196, 198
BstHHI GCGC 1 cut(s) 198
BstKTI GATC 2 cut(s) 504, 644
BstMAI GTCTC 2 cut(s) 380, 768
BstMBI GATC 2 cut(s) 501, 641
BstMWI GCNNNNNNNGC 7 cut(s) 29, 38, 467, 476, 761, 860, 946
BstNI CCWGG 3 cut(s) 306, 534, 716
BstNSI RCATGY 1 cut(s) 940
BstPAI GACNNNNGTC 1 cut(s) 197
BstSCI CCNGG 3 cut(s) 304, 532, 714
BstSFI CTRYAG 2 cut(s) 465, 592
BstUI CGCG 2 cut(s) 196, 198
BstV1I GCAGC 3 cut(s) 19, 260, 773
BsuI GTATCC 1 cut(s) 966
BsuRI GGCC 1 cut(s) 584
BtsCI GGATG 4 cut(s) 133, 148, 637, 926
BtsI GCAGTG 2 cut(s) 436, 534
BtsIMutI CAGTG 3 cut(s) 436, 523, 534
CaiI CAGNNNCTG 2 cut(s) 464, 533
CfoI GCGC 1 cut(s) 198
Cfr13I GGNCC 1 cut(s) 367
CseI GACGC 2 cut(s) 187, 202
Csp6I GTAC 2 cut(s) 50, 451
CviAII CATG 3 cut(s) 259, 586, 937
CviQI GTAC 2 cut(s) 50, 451
DdeI CTNAG 3 cut(s) 384, 563, 741
DpnI GATC 2 cut(s) 503, 643
DpnII GATC 2 cut(s) 501, 641
DraIII CACNNNGTG 1 cut(s) 95
Eam1104I CTCTTC 2 cut(s) 174, 844
EarI CTCTTC 2 cut(s) 174, 844
Eco32I GATATC 1 cut(s) 838
Eco47I GGWCC 1 cut(s) 367
EcoRII CCWGG 3 cut(s) 304, 532, 714
EcoRV GATATC 1 cut(s) 838
FaeI CATG 3 cut(s) 262, 589, 940
FaqI GGGAC 2 cut(s) 205, 380
FatI CATG 3 cut(s) 258, 585, 936
FauNDI CATATG 1 cut(s) 103
Fnu4HI GCNGC 3 cut(s) 33, 249, 762
FokI GGATG 4 cut(s) 140, 155, 644, 933
Fsp4HI GCNGC 3 cut(s) 33, 249, 762
FspBI CTAG 1 cut(s) 443
GlaI GCGC 1 cut(s) 197
GluI GCNGC 3 cut(s) 33, 249, 762
GsaI CCCAGC 2 cut(s) 127, 576
GsuI CTGGAG 2 cut(s) 555, 852
HaeIII GGCC 1 cut(s) 584
HgaI GACGC 2 cut(s) 187, 202
HhaI GCGC 1 cut(s) 198
Hin1II CATG 3 cut(s) 262, 589, 940
Hin6I GCGC 1 cut(s) 196
HinP1I GCGC 1 cut(s) 196
HincII GTYRAC 1 cut(s) 256
HindII GTYRAC 1 cut(s) 256
HindIII AAGCTT 1 cut(s) 964
HinfI GANTC 5 cut(s) 285, 373, 659, 776, 873
HphI GGTGA 1 cut(s) 771
Hpy166II GTNNAC 4 cut(s) 211, 256, 682, 811
Hpy188I TCNGA 3 cut(s) 385, 418, 827
Hpy188III TCNNGA 5 cut(s) 171, 511, 808, 870, 983
Hpy8I GTNNAC 4 cut(s) 211, 256, 682, 811
HpyAV CCTTC 4 cut(s) 130, 218, 316, 969
HpyCH4III ACNGT 5 cut(s) 391, 407, 466, 518, 722
HpyCH4IV ACGT 1 cut(s) 449
HpyCH4V TGCA 6 cut(s) 107, 494, 527, 550, 761, 816
HpyF10VI GCNNNNNNNGC 7 cut(s) 29, 38, 467, 476, 761, 860, 946
HpyF3I CTNAG 3 cut(s) 384, 563, 741
HpySE526I ACGT 1 cut(s) 449
Hsp92II CATG 3 cut(s) 262, 589, 940
HspAI GCGC 1 cut(s) 196
Kzo9I GATC 2 cut(s) 501, 641
LguI GCTCTTC 2 cut(s) 174, 844
LmnI GCTCC 4 cut(s) 611, 752, 860, 1028
Lsp1109I GCAGC 3 cut(s) 19, 260, 773
LweI GCATC 2 cut(s) 422, 988
MaeI CTAG 1 cut(s) 443
MaeII ACGT 1 cut(s) 449
MaeIII GTNAC 2 cut(s) 705, 926
MalI GATC 2 cut(s) 503, 643
MboI GATC 2 cut(s) 501, 641
MboII GAAGA 4 cut(s) 191, 293, 800, 861
MfeI CAATTG 1 cut(s) 756
MhlI GDGCHC 2 cut(s) 616, 1006
MluCI AATT 7 cut(s) 240, 291, 309, 336, 393, 756, 986
MlyI GAGTC 3 cut(s) 294, 770, 882
MnlI CCTC 5 cut(s) 469, 530, 924, 939, 945
MslI CAYNNNNRTG 1 cut(s) 206
MspA1I CMGCKG 1 cut(s) 123
MspR9I CCNGG 3 cut(s) 306, 534, 716
MunI CAATTG 1 cut(s) 756
Mva1269I GAATGC 1 cut(s) 552
MvaI CCWGG 3 cut(s) 306, 534, 716
MvnI CGCG 2 cut(s) 196, 198
MwoI GCNNNNNNNGC 7 cut(s) 29, 38, 467, 476, 761, 860, 946
NdeI CATATG 1 cut(s) 103
NdeII GATC 2 cut(s) 501, 641
NlaIII CATG 3 cut(s) 262, 589, 940
NlaIV GGNNCC 2 cut(s) 264, 368
NmuCI GTSAC 2 cut(s) 705, 926
NspI RCATGY 1 cut(s) 940
PciSI GCTCTTC 2 cut(s) 174, 844
PcsI WCGNNNNNNNCGW 1 cut(s) 195
PctI GAATGC 1 cut(s) 552
PfeI GAWTC 2 cut(s) 373, 659
PfoI TCCNGGA 1 cut(s) 304
PkrI GCNGC 3 cut(s) 34, 250, 763
PleI GAGTC 3 cut(s) 293, 770, 881
PpsI GAGTC 3 cut(s) 293, 770, 881
PshAI GACNNNNGTC 1 cut(s) 197
Psp6I CCWGG 3 cut(s) 304, 532, 714
PspFI CCCAGC 2 cut(s) 123, 572
PspGI CCWGG 3 cut(s) 304, 532, 714
PspN4I GGNNCC 2 cut(s) 264, 368
PspPI GGNCC 1 cut(s) 367
PstNI CAGNNNCTG 2 cut(s) 464, 533
PvuII CAGCTG 1 cut(s) 123
RsaI GTAC 2 cut(s) 51, 452
RsaNI GTAC 2 cut(s) 50, 451
RseI CAYNNNNRTG 1 cut(s) 206
SapI GCTCTTC 2 cut(s) 174, 844
SatI GCNGC 3 cut(s) 33, 249, 762
Sau3AI GATC 2 cut(s) 501, 641
Sau96I GGNCC 1 cut(s) 367
ScaI AGTACT 1 cut(s) 51
SchI GAGTC 3 cut(s) 294, 770, 882
ScrFI CCNGG 3 cut(s) 306, 534, 716
SduI GDGCHC 2 cut(s) 616, 1006
SfaNI GCATC 2 cut(s) 422, 988
SfcI CTRYAG 2 cut(s) 465, 592
SinI GGWCC 1 cut(s) 367
SmiMI CAYNNNNRTG 1 cut(s) 206
Sse9I AATT 7 cut(s) 240, 291, 309, 336, 393, 756, 986
SsiI CCGC 1 cut(s) 521
SspMI CTAG 1 cut(s) 443
StyD4I CCNGG 3 cut(s) 304, 532, 714
TaaI ACNGT 5 cut(s) 391, 407, 466, 518, 722
TaiI ACGT 1 cut(s) 452
TaqI TCGA 2 cut(s) 376, 510
TasI AATT 7 cut(s) 240, 291, 309, 336, 393, 756, 986
TatI WGTACW 1 cut(s) 49
TfiI GAWTC 2 cut(s) 373, 659
TscAI CASTG 3 cut(s) 436, 523, 534
TseFI GTSAC 2 cut(s) 705, 926
TseI GCWGC 3 cut(s) 32, 248, 761
Tsp45I GTSAC 2 cut(s) 705, 926
TspDTI ATGAA 3 cut(s) 192, 676, 801
TspGWI ACGGA 1 cut(s) 765
TspRI CASTG 3 cut(s) 436, 523, 534
VpaK11BI GGWCC 1 cut(s) 367
XapI RAATTY 2 cut(s) 291, 309
XceI RCATGY 1 cut(s) 940
XspI CTAG 1 cut(s) 443
ZrmI AGTACT 1 cut(s) 51
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.