RLG00000034927

Pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0013350
Physical Location & Seq
Reverse (-)
57185427 .. 57187549
2123 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000034927

Sequence Viewer

Length: 1194 bp
ATGGAATTGGGCGTTTTTAGTGTCTCTGTTGCTGCTGGTGCTGGTCTAGTACTGCTGGTTTTGTTGGGTGGTTTTCAGTTGGGTTTCTCACAAAGTGAAGCATCTGCAAAGAGTTATATTAGCTGGGATGATTTGAAGGTGGATGAGCAGAAGGTAATTGATTTGAACTCTAGAAATGAAGAGCAAAACGGGACGCGCATCATTGTGGTGGACAAGAATGGAGAAGGAGACTCTATAACAGTTCAAGGAGCAGTTGACATGGTTCCACACCATAACACCCAAAGAGTCAAAATATACATTCTTCCAGGAATTTATAGAGAGAAGGTGCTTATACCAATTACCAAGCCATACATATCATTTATAGGGGACCAGAATCGAACTTCTGAGACTGTAATTACTTGGAACAACAAGGCATCTGATAAGGACAGCAATGGCTTTGAACTAGGAACGTACAGAACAGCAACTGTAGCCATAGAGGCTGACTACTTTTGTGCAACAGGGATCACTTTCGAGAACAGTGTGGTTGCAGTGCCTGGAGGATATGGAATGCAAGGAGTGGCTCTTAGAGTTGCTGGGGATAAGGCCATGTTCTATAGAGTAAGGGTCTTGGGAGCACAAGATACACTATTGGATGAGAATGGATCGCACTACTTCTTCGAATCCCACATTCAAGGAAGTGTGGACTTCATATTTGGCAAATCAAGGTCACTCTACCAGGTACAATTCTCAATTCCTAGCATTTCCATATGTAACTTGCTCTTTAAGTTTCTTATGATTCTGATGATGTTTGAATTGGTTTCTTACAACCAGGACTGTGTTATCCAATCAACTGCTAAGACCTATGGAGCAATTGCAGCTCATCATAGAGACTCACCAAATGAAGATACAGGGTTCTCTTTCGTGAACTGCAAAATCATCGGAACTGGAGATATCTACTTGGGAAGAGCTTGGGGAGCTTATTCAAGAGTCATATATTCCTATTGTCATATTGACAGTATAATAACTCCTCTGGGATGGAGTGACTGGAACATGCCACCAAGGCAGAGGACTGTGGAATTCGGAGAATACCAATGCAGAGGCAAAGGAGCAGATAGAAGAGGCAGGGTACCATGGTTGAAGTCTTTTAGTCCGGCCGAGATAACCCCTTTTCTGGATACCAAATTCATATATGGAGAGCAATGGCTTAGACTATAG

Protein Analysis

398

Amino Acids

44.33

Weight (kDa)

5.86

Isoelectric Point (pI)

26.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 68 - 239 1.2e-35 Pectinesterase
Pectinesterase PF01095 275 - 391 4.7e-21 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013350)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G55590 AT5G55590
fragaria_vesca FvH4_3g29490
malus_domestica MD03G1186900.v1.1 MD11G1201900.v1.1
prunus_persica Prupe.4G239700_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0054371
rosa_laevigata RLG00000034927
rosa_multiflora Rmu_sc0005705.1_g000023 Rmu_sc0023043.1_g000001
rosa_roxburghii Rroxscaffold_1G00026500
rosa_rugosa Rorug05G0285900
rosa_samantha Rh5AG356400 Rh5BG368000 Rh5CG391300 Rh5DG382200
rosa_wichuraiana Rw5G033480 Rw5G033650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1105
AccB1I GGYRCC 1 cut(s) 1105
AccII CGCG 1 cut(s) 196
AclWI GGATC 2 cut(s) 509, 649
AcoI YGGCCR 1 cut(s) 1131
AcsI RAATTY 3 cut(s) 309, 1055, 1160
AdeI CACNNNGTG 1 cut(s) 95
AfaI GTAC 4 cut(s) 51, 452, 720, 1107
AfiI CCNNNNNNNGG 1 cut(s) 1150
AgsI TTSAA 8 cut(s) 136, 166, 245, 440, 671, 791, 963, 1117
AjnI CCWGG 4 cut(s) 304, 532, 714, 807
AluBI AGCT 4 cut(s) 123, 857, 947, 956
AluI AGCT 4 cut(s) 123, 857, 947, 956
Alw21I GWGCWC 1 cut(s) 616
Alw26I GTCTC 4 cut(s) 28, 222, 380, 861
AlwI GGATC 2 cut(s) 509, 649
AlwNI CAGNNNCTG 2 cut(s) 464, 533
AoxI GGCC 2 cut(s) 582, 1131
ApeKI GCWGC 2 cut(s) 32, 854
ApoI RAATTY 3 cut(s) 309, 1055, 1160
ArsI GACNNNNNNTTYG 2 cut(s) 674, 706
Asp718I GGTACC 1 cut(s) 1105
AspLEI GCGC 1 cut(s) 198
AspS9I GGNCC 1 cut(s) 367
AsuHPI GGTGA 1 cut(s) 864
AsuII TTCGAA 1 cut(s) 657
AvaII GGWCC 1 cut(s) 367
BaeI ACNNNNGTAYC 2 cut(s) 1097, 1130
BanI GGYRCC 1 cut(s) 1105
Bbv12I GWGCWC 1 cut(s) 616
BbvI GCAGC 2 cut(s) 19, 866
BccI CCATC 1 cut(s) 1008
BcgI CGANNNNNNTGC 2 cut(s) 898, 932
BciT130I CCWGG 4 cut(s) 306, 534, 716, 809
BciVI GTATCC 1 cut(s) 1147
BcoDI GTCTC 4 cut(s) 28, 222, 380, 861
BfaI CTAG 4 cut(s) 47, 171, 443, 735
BfmI CTRYAG 3 cut(s) 465, 592, 1190
BfuI GTATCC 1 cut(s) 1147
BglI GCCNNNNNGGC 2 cut(s) 476, 1039
BisI GCNGC 2 cut(s) 33, 855
BlsI GCNGC 2 cut(s) 34, 856
BmcAI AGTACT 1 cut(s) 51
Bme1390I CCNGG 4 cut(s) 306, 534, 716, 809
Bme18I GGWCC 1 cut(s) 367
BmgT120I GGNCC 1 cut(s) 367
BmiI GGNNCC 3 cut(s) 264, 368, 1107
BmrFI CCNGG 4 cut(s) 306, 534, 716, 809
BmsI GCATC 3 cut(s) 110, 207, 422
BpmI CTGGAG 2 cut(s) 555, 945
Bpu14I TTCGAA 1 cut(s) 657
BsaJI CCNNGG 2 cut(s) 1037, 1109
Bsc4I CCNNNNNNNGG 1 cut(s) 1150
Bse1I ACTGG 2 cut(s) 928, 1028
Bse3DI GCAATG 2 cut(s) 436, 1184
BseBI CCWGG 4 cut(s) 306, 534, 716, 809
BseDI CCNNGG 2 cut(s) 1037, 1109
BseGI GGATG 4 cut(s) 133, 148, 637, 1019
BseLI CCNNNNNNNGG 1 cut(s) 1150
BseMI GCAATG 2 cut(s) 436, 1184
BseMII CTCAG 1 cut(s) 375
BseNI ACTGG 2 cut(s) 928, 1028
BseRI GAGGAG 1 cut(s) 996
BseX3I CGGCCG 1 cut(s) 1131
BseXI GCAGC 2 cut(s) 19, 866
BseYI CCCAGC 2 cut(s) 123, 572
Bsh1236I CGCG 1 cut(s) 196
Bsh1285I CGRYCG 1 cut(s) 1134
BshFI GGCC 2 cut(s) 584, 1133
BshNI GGYRCC 1 cut(s) 1105
BsiEI CGRYCG 1 cut(s) 1134
BsiHKAI GWGCWC 1 cut(s) 616
BsiSI CCGG 1 cut(s) 1130
BslFI GGGAC 2 cut(s) 205, 380
BslI CCNNNNNNNGG 1 cut(s) 1150
BsmAI GTCTC 4 cut(s) 28, 222, 380, 861
BsmFI GGGAC 2 cut(s) 205, 380
BsmI GAATGC 1 cut(s) 552
BsnI GGCC 2 cut(s) 584, 1133
Bsp119I TTCGAA 1 cut(s) 657
Bsp1286I GDGCHC 1 cut(s) 616
Bsp143I GATC 2 cut(s) 501, 641
Bsp19I CCATGG 1 cut(s) 1109
BspANI GGCC 2 cut(s) 584, 1133
BspCNI CTCAG 1 cut(s) 376
BspFNI CGCG 1 cut(s) 196
BspLI GGNNCC 3 cut(s) 264, 368, 1107
BspPI GGATC 2 cut(s) 509, 649
BspQI GCTCTTC 2 cut(s) 174, 937
BspT104I TTCGAA 1 cut(s) 657
BspT107I GGYRCC 1 cut(s) 1105
BsrDI GCAATG 2 cut(s) 436, 1184
BsrI ACTGG 2 cut(s) 928, 1028
BssECI CCNNGG 2 cut(s) 1037, 1109
BssMI GATC 2 cut(s) 501, 641
BssT1I CCWWGG 2 cut(s) 1037, 1109
Bst2UI CCWGG 4 cut(s) 306, 534, 716, 809
Bst4CI ACNGT 7 cut(s) 241, 391, 466, 518, 815, 995, 1051
Bst6I CTCTTC 3 cut(s) 174, 937, 1090
BstBI TTCGAA 1 cut(s) 657
BstDEI CTNAG 4 cut(s) 384, 563, 834, 1184
BstDSI CCRYGG 1 cut(s) 1109
BstF5I GGATG 4 cut(s) 133, 148, 637, 1019
BstFNI CGCG 1 cut(s) 196
BstHHI GCGC 1 cut(s) 198
BstKTI GATC 2 cut(s) 504, 644
BstMAI GTCTC 4 cut(s) 28, 222, 380, 861
BstMBI GATC 2 cut(s) 501, 641
BstMCI CGRYCG 1 cut(s) 1134
BstMWI GCNNNNNNNGC 6 cut(s) 38, 467, 476, 854, 953, 1039
BstNI CCWGG 4 cut(s) 306, 534, 716, 809
BstNSI RCATGY 1 cut(s) 1033
BstSCI CCNGG 4 cut(s) 304, 532, 714, 807
BstSFI CTRYAG 3 cut(s) 465, 592, 1190
BstUI CGCG 1 cut(s) 196
BstV1I GCAGC 2 cut(s) 19, 866
BstZI CGGCCG 1 cut(s) 1131
BsuI GTATCC 1 cut(s) 1147
BsuRI GGCC 2 cut(s) 584, 1133
BtgI CCRYGG 1 cut(s) 1109
BtsCI GGATG 4 cut(s) 133, 148, 637, 1019
BtsI GCAGTG 1 cut(s) 534
BtsIMutI CAGTG 2 cut(s) 523, 534
CaiI CAGNNNCTG 2 cut(s) 464, 533
CfoI GCGC 1 cut(s) 198
Cfr13I GGNCC 1 cut(s) 367
CseI GACGC 1 cut(s) 202
CsiI ACCWGGT 1 cut(s) 714
Csp6I GTAC 4 cut(s) 50, 451, 719, 1106
CviAII CATG 4 cut(s) 259, 586, 1030, 1110
CviQI GTAC 4 cut(s) 50, 451, 719, 1106
DdeI CTNAG 4 cut(s) 384, 563, 834, 1184
DpnI GATC 2 cut(s) 503, 643
DpnII GATC 2 cut(s) 501, 641
DraIII CACNNNGTG 1 cut(s) 95
EaeI YGGCCR 1 cut(s) 1131
EagI CGGCCG 1 cut(s) 1131
Eam1104I CTCTTC 3 cut(s) 174, 937, 1090
EarI CTCTTC 3 cut(s) 174, 937, 1090
EclXI CGGCCG 1 cut(s) 1131
Eco130I CCWWGG 2 cut(s) 1037, 1109
Eco32I GATATC 1 cut(s) 931
Eco47I GGWCC 1 cut(s) 367
Eco52I CGGCCG 1 cut(s) 1131
EcoRI GAATTC 1 cut(s) 1055
EcoRII CCWGG 4 cut(s) 304, 532, 714, 807
EcoRV GATATC 1 cut(s) 931
EcoT14I CCWWGG 2 cut(s) 1037, 1109
ErhI CCWWGG 2 cut(s) 1037, 1109
FaeI CATG 4 cut(s) 262, 589, 1033, 1113
FaqI GGGAC 2 cut(s) 205, 380
FatI CATG 4 cut(s) 258, 585, 1029, 1109
FauNDI CATATG 1 cut(s) 746
Fnu4HI GCNGC 2 cut(s) 33, 855
FokI GGATG 4 cut(s) 140, 155, 644, 1026
Fsp4HI GCNGC 2 cut(s) 33, 855
FspBI CTAG 4 cut(s) 47, 171, 443, 735
GlaI GCGC 1 cut(s) 197
GluI GCNGC 2 cut(s) 33, 855
GsaI CCCAGC 2 cut(s) 127, 576
GsuI CTGGAG 2 cut(s) 555, 945
HaeIII GGCC 2 cut(s) 584, 1133
HapII CCGG 1 cut(s) 1130
HgaI GACGC 1 cut(s) 202
HhaI GCGC 1 cut(s) 198
Hin1II CATG 4 cut(s) 262, 589, 1033, 1113
Hin6I GCGC 1 cut(s) 196
HinP1I GCGC 1 cut(s) 196
HincII GTYRAC 1 cut(s) 256
HindII GTYRAC 1 cut(s) 256
HinfI GANTC 7 cut(s) 230, 285, 373, 659, 775, 869, 966
HpaII CCGG 1 cut(s) 1130
HphI GGTGA 1 cut(s) 864
Hpy166II GTNNAC 4 cut(s) 211, 256, 682, 904
Hpy188I TCNGA 5 cut(s) 385, 418, 780, 920, 1061
Hpy188III TCNNGA 5 cut(s) 171, 511, 901, 963, 1151
Hpy8I GTNNAC 4 cut(s) 211, 256, 682, 904
HpyAV CCTTC 4 cut(s) 130, 145, 218, 316
HpyCH4III ACNGT 7 cut(s) 241, 391, 466, 518, 815, 995, 1051
HpyCH4IV ACGT 1 cut(s) 449
HpyCH4V TGCA 7 cut(s) 107, 494, 527, 550, 854, 909, 1074
HpyF10VI GCNNNNNNNGC 6 cut(s) 38, 467, 476, 854, 953, 1039
HpyF3I CTNAG 4 cut(s) 384, 563, 834, 1184
HpySE526I ACGT 1 cut(s) 449
Hsp92II CATG 4 cut(s) 262, 589, 1033, 1113
HspAI GCGC 1 cut(s) 196
KpnI GGTACC 1 cut(s) 1109
Kzo9I GATC 2 cut(s) 501, 641
LguI GCTCTTC 2 cut(s) 174, 937
LmnI GCTCC 5 cut(s) 248, 611, 845, 953, 1085
Lsp1109I GCAGC 2 cut(s) 19, 866
LweI GCATC 3 cut(s) 110, 207, 422
MabI ACCWGGT 1 cut(s) 714
MaeI CTAG 4 cut(s) 47, 171, 443, 735
MaeII ACGT 1 cut(s) 449
MaeIII GTNAC 3 cut(s) 705, 749, 1019
MalI GATC 2 cut(s) 503, 643
MboI GATC 2 cut(s) 501, 641
MboII GAAGA 6 cut(s) 191, 293, 646, 893, 954, 1107
MfeI CAATTG 1 cut(s) 849
MhlI GDGCHC 1 cut(s) 616
MlyI GAGTC 4 cut(s) 224, 294, 863, 975
MnlI CCTC 6 cut(s) 469, 530, 1017, 1038, 1070, 1091
MseI TTAA 1 cut(s) 762
MslI CAYNNNNRTG 2 cut(s) 203, 206
MspI CCGG 1 cut(s) 1130
MspR9I CCNGG 4 cut(s) 306, 534, 716, 809
MunI CAATTG 1 cut(s) 849
Mva1269I GAATGC 1 cut(s) 552
MvaI CCWGG 4 cut(s) 306, 534, 716, 809
MvnI CGCG 1 cut(s) 196
MwoI GCNNNNNNNGC 6 cut(s) 38, 467, 476, 854, 953, 1039
NcoI CCATGG 1 cut(s) 1109
NdeI CATATG 1 cut(s) 746
NdeII GATC 2 cut(s) 501, 641
NlaIII CATG 4 cut(s) 262, 589, 1033, 1113
NlaIV GGNNCC 3 cut(s) 264, 368, 1107
NmeAIII GCCGAG 1 cut(s) 1159
NmuCI GTSAC 2 cut(s) 705, 1019
NspI RCATGY 1 cut(s) 1033
NspV TTCGAA 1 cut(s) 657
PciSI GCTCTTC 2 cut(s) 174, 937
PctI GAATGC 1 cut(s) 552
PfeI GAWTC 3 cut(s) 373, 659, 775
PfoI TCCNGGA 1 cut(s) 304
PkrI GCNGC 2 cut(s) 34, 856
PleI GAGTC 4 cut(s) 224, 293, 863, 974
PpsI GAGTC 4 cut(s) 224, 293, 863, 974
Psp6I CCWGG 4 cut(s) 304, 532, 714, 807
PspFI CCCAGC 2 cut(s) 123, 572
PspGI CCWGG 4 cut(s) 304, 532, 714, 807
PspN4I GGNNCC 3 cut(s) 264, 368, 1107
PspPI GGNCC 1 cut(s) 367
PstNI CAGNNNCTG 2 cut(s) 464, 533
RsaI GTAC 4 cut(s) 51, 452, 720, 1107
RsaNI GTAC 4 cut(s) 50, 451, 719, 1106
RseI CAYNNNNRTG 2 cut(s) 203, 206
SapI GCTCTTC 2 cut(s) 174, 937
SaqAI TTAA 1 cut(s) 762
SatI GCNGC 2 cut(s) 33, 855
Sau3AI GATC 2 cut(s) 501, 641
Sau96I GGNCC 1 cut(s) 367
ScaI AGTACT 1 cut(s) 51
SchI GAGTC 4 cut(s) 224, 294, 863, 975
ScrFI CCNGG 4 cut(s) 306, 534, 716, 809
SduI GDGCHC 1 cut(s) 616
SexAI ACCWGGT 1 cut(s) 714
SfaNI GCATC 3 cut(s) 110, 207, 422
SfcI CTRYAG 3 cut(s) 465, 592, 1190
SfuI TTCGAA 1 cut(s) 657
SinI GGWCC 1 cut(s) 367
SmiMI CAYNNNNRTG 2 cut(s) 203, 206
SspMI CTAG 4 cut(s) 47, 171, 443, 735
StyD4I CCNGG 4 cut(s) 304, 532, 714, 807
StyI CCWWGG 2 cut(s) 1037, 1109
TaaI ACNGT 7 cut(s) 241, 391, 466, 518, 815, 995, 1051
TaiI ACGT 1 cut(s) 452
TaqI TCGA 3 cut(s) 376, 510, 657
TatI WGTACW 1 cut(s) 49
TfiI GAWTC 3 cut(s) 373, 659, 775
Tru1I TTAA 1 cut(s) 762
Tru9I TTAA 1 cut(s) 762
TscAI CASTG 2 cut(s) 523, 534
TseFI GTSAC 2 cut(s) 705, 1019
TseI GCWGC 2 cut(s) 32, 854
Tsp45I GTSAC 2 cut(s) 705, 1019
TspDTI ATGAA 4 cut(s) 192, 676, 894, 1153
TspRI CASTG 2 cut(s) 523, 534
VpaK11BI GGWCC 1 cut(s) 367
XapI RAATTY 3 cut(s) 309, 1055, 1160
XbaI TCTAGA 1 cut(s) 170
XceI RCATGY 1 cut(s) 1033
XspI CTAG 4 cut(s) 47, 171, 443, 735
ZrmI AGTACT 1 cut(s) 51
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.