FvH4_3g29490

Pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0013350
Physical Location & Seq
Reverse (-)
22541900 .. 22544156
2257 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g29490.t1

Sequence Viewer

Length: 1101 bp
ATGGGATTGGGTGTTTATAGTGCTTCTGTTGCTGCTTGTGCTGGTTTAGTACTACTGCTTCTGTTGAGTGGTTTTCAGTTGGGTTTGTCACAAAGTGAAGCATATGCAAGGAGTTATATTAGCTGGGATGATTTGAAGGTTGATGAGCAGATGGTTATCGGTTTGAACTCTAGAAATGAACAGAAAAACGAGACGTCCGTCATTGTTGTGGACAAGAATGGAGGGGGAAATTCTATGACAGTTCAAGGAGCAGTTGACATGGTTCCAGATCACAACAAACAAAGATACAAGATATACATTCTTCCAGGAATATATAGAGAGAAGGTGCGCATACCAATTACCAAACCATACATATCATTTATAGGCAACCAGAATCAAACTTCCGAGACTGTAATTACATGGAACGACAAGGCATCTGATAAGGACAGCAATGGCTTTGAACTAGGAACGTACAGAACAGCAACTGTAGCCATAGAGGCTGATTACTTTTGTGCAACAGGGATCACTTTCGAGAACAGTGTGGCTGCAGTGCCTGGAGGATATGGAATGCAAGGAGTAGCTCTTCGAGTTGCTGGTGATAAGGCCATGTTCTATAAAGTAAGGGTCTTGGGAGCACAAGACACACTATTGGATGAGAATGGATCACACTACTACTACCAATCCCACATCCAAGGAAGTGTGGACTTCATATTTGGCAAATCAAGGTCACTCTACCAGGACTGTGTTATCCAATCAACAGCTAAGACCTATGGAGCCATTGCAGCTCATCATAGAGACTCAGCAAATGAAGATACAGGGTTCTCTTTCGTGAACTGCACAATCATCGGAACTGGATATATCTACTTGGGAAGAGCTTGGGGAGCTTATTCAAGAGTAATATATTCCTACTGTGGTATCGACAATATAATAACTCCTCCGGGATGGAGTGATTGGAACATCCCACCAAGACAGAGGACTGTGGAATTCGGAGAATACGAATGCAGAGGCAAAGGAGCAGATAAAAGAGGCAGGGTACCATGGTTGAAGTCTTTCAGTCCTGCGGAGATAGTCCCTTTTCTGGATACCAAGTTCATTTATGGAGAGCAATGGCTTAGACTATAG

Protein Analysis

367

Amino Acids

40.57

Weight (kDa)

6.24

Isoelectric Point (pI)

31.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 68 - 360 5.6e-63 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013350)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G55590 AT5G55590
fragaria_vesca FvH4_3g29490
malus_domestica MD03G1186900.v1.1 MD11G1201900.v1.1
prunus_persica Prupe.4G239700_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0054371
rosa_laevigata RLG00000034927
rosa_multiflora Rmu_sc0005705.1_g000023 Rmu_sc0023043.1_g000001
rosa_roxburghii Rroxscaffold_1G00026500
rosa_rugosa Rorug05G0285900
rosa_samantha Rh5AG356400 Rh5BG368000 Rh5CG391300 Rh5DG382200
rosa_wichuraiana Rw5G033480 Rw5G033650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 197
Acc16I TGCGCA 1 cut(s) 329
Acc65I GGTACC 1 cut(s) 1012
AccB1I GGYRCC 1 cut(s) 1012
AciI CCGC 1 cut(s) 1040
AclWI GGATC 2 cut(s) 509, 649
AcsI RAATTY 2 cut(s) 229, 962
AcyI GRCGYC 1 cut(s) 194
AdeI CACNNNGTG 1 cut(s) 95
AfaI GTAC 3 cut(s) 51, 452, 1014
AfiI CCNNNNNNNGG 1 cut(s) 1057
AgsI TTSAA 6 cut(s) 136, 166, 245, 440, 870, 1024
AjnI CCWGG 3 cut(s) 304, 532, 714
AloI GAACNNNNNNTCC 2 cut(s) 1052, 1084
AluBI AGCT 6 cut(s) 123, 560, 740, 764, 854, 863
AluI AGCT 6 cut(s) 123, 560, 740, 764, 854, 863
Alw21I GWGCWC 1 cut(s) 616
Alw26I GTCTC 3 cut(s) 185, 380, 768
AlwI GGATC 2 cut(s) 509, 649
AlwNI CAGNNNCTG 2 cut(s) 464, 533
AoxI GGCC 1 cut(s) 582
ApeKI GCWGC 3 cut(s) 32, 524, 761
ApoI RAATTY 2 cut(s) 229, 962
ArsI GACNNNNNNTTYG 2 cut(s) 674, 706
Asp700I GAANNNNTTC 1 cut(s) 1028
Asp718I GGTACC 1 cut(s) 1012
AspLEI GCGC 1 cut(s) 330
AsuC2I CCSGG 1 cut(s) 918
AsuHPI GGTGA 1 cut(s) 587
BaeI ACNNNNGTAYC 4 cut(s) 877, 910, 1004, 1037
BanI GGYRCC 1 cut(s) 1012
BarI GAAGNNNNNNTAC 2 cut(s) 42, 74
Bbv12I GWGCWC 1 cut(s) 616
BbvI GCAGC 3 cut(s) 19, 511, 773
BccI CCATC 2 cut(s) 145, 915
BcgI CGANNNNNNTGC 2 cut(s) 805, 839
BciT130I CCWGG 3 cut(s) 306, 534, 716
BciVI GTATCC 1 cut(s) 1054
BcnI CCSGG 1 cut(s) 918
BcoDI GTCTC 3 cut(s) 185, 380, 768
BfaI CTAG 2 cut(s) 171, 443
BfmI CTRYAG 3 cut(s) 465, 525, 1097
BfuI GTATCC 1 cut(s) 1054
BglI GCCNNNNNGGC 1 cut(s) 476
BisI GCNGC 3 cut(s) 33, 525, 762
BlsI GCNGC 3 cut(s) 34, 526, 763
BmcAI AGTACT 1 cut(s) 51
Bme1390I CCNGG 4 cut(s) 306, 534, 716, 918
BmiI GGNNCC 3 cut(s) 264, 754, 1014
BmrFI CCNGG 4 cut(s) 306, 534, 716, 918
BmsI GCATC 1 cut(s) 422
BoxI GACNNNNGTC 1 cut(s) 197
BpmI CTGGAG 1 cut(s) 555
BpuMI CCSGG 1 cut(s) 918
BsaBI GATNNNNATC 1 cut(s) 155
BsaHI GRCGYC 1 cut(s) 194
BsaJI CCNNGG 2 cut(s) 670, 1016
Bsc4I CCNNNNNNNGG 1 cut(s) 1057
Bse1I ACTGG 1 cut(s) 835
Bse3DI GCAATG 3 cut(s) 436, 756, 1091
Bse8I GATNNNNATC 1 cut(s) 155
BseBI CCWGG 3 cut(s) 306, 534, 716
BseDI CCNNGG 2 cut(s) 670, 1016
BseGI GGATG 5 cut(s) 133, 637, 666, 926, 936
BseJI GATNNNNATC 1 cut(s) 155
BseLI CCNNNNNNNGG 1 cut(s) 1057
BseMI GCAATG 3 cut(s) 436, 756, 1091
BseMII CTCAG 1 cut(s) 792
BseNI ACTGG 1 cut(s) 835
BseRI GAGGAG 1 cut(s) 903
BseXI GCAGC 3 cut(s) 19, 511, 773
BseYI CCCAGC 1 cut(s) 123
BsgI GTGCAG 1 cut(s) 799
BshFI GGCC 1 cut(s) 584
BshNI GGYRCC 1 cut(s) 1012
BsiHKAI GWGCWC 1 cut(s) 616
BsiSI CCGG 1 cut(s) 917
BslFI GGGAC 1 cut(s) 1034
BslI CCNNNNNNNGG 1 cut(s) 1057
BsmAI GTCTC 3 cut(s) 185, 380, 768
BsmBI CGTCTC 1 cut(s) 185
BsmFI GGGAC 1 cut(s) 1034
BsmI GAATGC 2 cut(s) 552, 983
BsnI GGCC 1 cut(s) 584
Bsp1286I GDGCHC 1 cut(s) 616
Bsp143I GATC 3 cut(s) 268, 501, 641
Bsp19I CCATGG 1 cut(s) 1016
BspACI CCGC 1 cut(s) 1040
BspANI GGCC 1 cut(s) 584
BspCNI CTCAG 1 cut(s) 791
BspLI GGNNCC 3 cut(s) 264, 754, 1014
BspMAI CTGCAG 1 cut(s) 529
BspPI GGATC 2 cut(s) 509, 649
BspQI GCTCTTC 2 cut(s) 567, 844
BspT107I GGYRCC 1 cut(s) 1012
BsrDI GCAATG 3 cut(s) 436, 756, 1091
BsrI ACTGG 1 cut(s) 835
BssECI CCNNGG 2 cut(s) 670, 1016
BssMI GATC 3 cut(s) 268, 501, 641
BssNI GRCGYC 1 cut(s) 194
BssT1I CCWWGG 2 cut(s) 670, 1016
Bst2UI CCWGG 3 cut(s) 306, 534, 716
Bst4CI ACNGT 7 cut(s) 241, 391, 466, 518, 722, 890, 958
Bst6I CTCTTC 2 cut(s) 567, 844
BstACI GRCGYC 1 cut(s) 194
BstDEI CTNAG 3 cut(s) 741, 778, 1091
BstDSI CCRYGG 1 cut(s) 1016
BstF5I GGATG 5 cut(s) 133, 637, 666, 926, 936
BstHHI GCGC 1 cut(s) 330
BstKTI GATC 3 cut(s) 271, 504, 644
BstMAI GTCTC 3 cut(s) 185, 380, 768
BstMBI GATC 3 cut(s) 268, 501, 641
BstMWI GCNNNNNNNGC 6 cut(s) 29, 38, 467, 476, 761, 860
BstNI CCWGG 3 cut(s) 306, 534, 716
BstPAI GACNNNNGTC 1 cut(s) 197
BstSCI CCNGG 4 cut(s) 304, 532, 714, 916
BstSFI CTRYAG 3 cut(s) 465, 525, 1097
BstV1I GCAGC 3 cut(s) 19, 511, 773
BsuI GTATCC 1 cut(s) 1054
BsuRI GGCC 1 cut(s) 584
BtgI CCRYGG 1 cut(s) 1016
BtsCI GGATG 5 cut(s) 133, 637, 666, 926, 936
BtsI GCAGTG 1 cut(s) 534
BtsIMutI CAGTG 2 cut(s) 523, 534
CaiI CAGNNNCTG 2 cut(s) 464, 533
CfoI GCGC 1 cut(s) 330
Csp6I GTAC 3 cut(s) 50, 451, 1013
CviAII CATG 4 cut(s) 259, 399, 586, 1017
CviQI GTAC 3 cut(s) 50, 451, 1013
DdeI CTNAG 3 cut(s) 741, 778, 1091
DpnI GATC 3 cut(s) 270, 503, 643
DpnII GATC 3 cut(s) 268, 501, 641
DraIII CACNNNGTG 1 cut(s) 95
Eam1104I CTCTTC 2 cut(s) 567, 844
EarI CTCTTC 2 cut(s) 567, 844
Eco130I CCWWGG 2 cut(s) 670, 1016
EcoRI GAATTC 1 cut(s) 962
EcoRII CCWGG 3 cut(s) 304, 532, 714
EcoT14I CCWWGG 2 cut(s) 670, 1016
ErhI CCWWGG 2 cut(s) 670, 1016
Esp3I CGTCTC 1 cut(s) 185
FaeI CATG 4 cut(s) 262, 402, 589, 1020
FaqI GGGAC 1 cut(s) 1034
FatI CATG 4 cut(s) 258, 398, 585, 1016
FauNDI CATATG 1 cut(s) 103
Fnu4HI GCNGC 3 cut(s) 33, 525, 762
FokI GGATG 5 cut(s) 140, 644, 653, 923, 933
Fsp4HI GCNGC 3 cut(s) 33, 525, 762
FspAI RTGCGCAY 1 cut(s) 329
FspBI CTAG 2 cut(s) 171, 443
FspI TGCGCA 1 cut(s) 329
GlaI GCGC 1 cut(s) 329
GluI GCNGC 3 cut(s) 33, 525, 762
GsaI CCCAGC 1 cut(s) 127
GsuI CTGGAG 1 cut(s) 555
HaeIII GGCC 1 cut(s) 584
HapII CCGG 1 cut(s) 917
HhaI GCGC 1 cut(s) 330
Hin1I GRCGYC 1 cut(s) 194
Hin1II CATG 4 cut(s) 262, 402, 589, 1020
Hin6I GCGC 1 cut(s) 328
HinP1I GCGC 1 cut(s) 328
HincII GTYRAC 1 cut(s) 256
HindII GTYRAC 1 cut(s) 256
HinfI GANTC 2 cut(s) 373, 776
HpaII CCGG 1 cut(s) 917
HphI GGTGA 1 cut(s) 587
Hpy166II GTNNAC 4 cut(s) 211, 256, 682, 811
Hpy188I TCNGA 4 cut(s) 385, 418, 827, 968
Hpy188III TCNNGA 6 cut(s) 171, 266, 511, 808, 870, 1058
Hpy8I GTNNAC 4 cut(s) 211, 256, 682, 811
HpyAV CCTTC 2 cut(s) 130, 316
HpyCH4III ACNGT 7 cut(s) 241, 391, 466, 518, 722, 890, 958
HpyCH4IV ACGT 2 cut(s) 194, 449
HpyCH4V TGCA 7 cut(s) 107, 494, 527, 550, 761, 816, 981
HpyF10VI GCNNNNNNNGC 6 cut(s) 29, 38, 467, 476, 761, 860
HpyF3I CTNAG 3 cut(s) 741, 778, 1091
HpySE526I ACGT 2 cut(s) 194, 449
Hsp92I GRCGYC 1 cut(s) 194
Hsp92II CATG 4 cut(s) 262, 402, 589, 1020
HspAI GCGC 1 cut(s) 328
KpnI GGTACC 1 cut(s) 1016
Kzo9I GATC 3 cut(s) 268, 501, 641
LguI GCTCTTC 2 cut(s) 567, 844
LmnI GCTCC 5 cut(s) 248, 611, 752, 860, 992
Lsp1109I GCAGC 3 cut(s) 19, 511, 773
LweI GCATC 1 cut(s) 422
MaeI CTAG 2 cut(s) 171, 443
MaeII ACGT 2 cut(s) 194, 449
MaeIII GTNAC 2 cut(s) 87, 705
MalI GATC 3 cut(s) 270, 503, 643
MboI GATC 3 cut(s) 268, 501, 641
MboII GAAGA 4 cut(s) 293, 554, 800, 861
MhlI GDGCHC 1 cut(s) 616
MluCI AATT 4 cut(s) 229, 336, 393, 962
MlyI GAGTC 1 cut(s) 770
MnlI CCTC 7 cut(s) 215, 469, 530, 924, 945, 977, 998
MroXI GAANNNNTTC 1 cut(s) 1028
MslI CAYNNNNRTG 1 cut(s) 206
MspI CCGG 1 cut(s) 917
MspR9I CCNGG 4 cut(s) 306, 534, 716, 918
Mva1269I GAATGC 2 cut(s) 552, 983
MvaI CCWGG 3 cut(s) 306, 534, 716
MwoI GCNNNNNNNGC 6 cut(s) 29, 38, 467, 476, 761, 860
NciI CCSGG 1 cut(s) 918
NcoI CCATGG 1 cut(s) 1016
NdeI CATATG 1 cut(s) 103
NdeII GATC 3 cut(s) 268, 501, 641
NlaIII CATG 4 cut(s) 262, 402, 589, 1020
NlaIV GGNNCC 3 cut(s) 264, 754, 1014
NmuCI GTSAC 2 cut(s) 87, 705
NsbI TGCGCA 1 cut(s) 329
PciSI GCTCTTC 2 cut(s) 567, 844
PcsI WCGNNNNNNNCGW 2 cut(s) 195, 972
PctI GAATGC 2 cut(s) 552, 983
PdmI GAANNNNTTC 1 cut(s) 1028
PfeI GAWTC 1 cut(s) 373
PfoI TCCNGGA 2 cut(s) 304, 916
PkrI GCNGC 3 cut(s) 34, 526, 763
PleI GAGTC 1 cut(s) 770
PpsI GAGTC 1 cut(s) 770
PshAI GACNNNNGTC 1 cut(s) 197
Psp6I CCWGG 3 cut(s) 304, 532, 714
PspFI CCCAGC 1 cut(s) 123
PspGI CCWGG 3 cut(s) 304, 532, 714
PspN4I GGNNCC 3 cut(s) 264, 754, 1014
PstI CTGCAG 1 cut(s) 529
PstNI CAGNNNCTG 2 cut(s) 464, 533
RsaI GTAC 3 cut(s) 51, 452, 1014
RsaNI GTAC 3 cut(s) 50, 451, 1013
RseI CAYNNNNRTG 1 cut(s) 206
SapI GCTCTTC 2 cut(s) 567, 844
SatI GCNGC 3 cut(s) 33, 525, 762
Sau3AI GATC 3 cut(s) 268, 501, 641
ScaI AGTACT 1 cut(s) 51
SchI GAGTC 1 cut(s) 770
ScrFI CCNGG 4 cut(s) 306, 534, 716, 918
SduI GDGCHC 1 cut(s) 616
SfaNI GCATC 1 cut(s) 422
SfcI CTRYAG 3 cut(s) 465, 525, 1097
SmiMI CAYNNNNRTG 1 cut(s) 206
Sse9I AATT 4 cut(s) 229, 336, 393, 962
SsiI CCGC 1 cut(s) 1040
SspMI CTAG 2 cut(s) 171, 443
StyD4I CCNGG 4 cut(s) 304, 532, 714, 916
StyI CCWWGG 2 cut(s) 670, 1016
TaaI ACNGT 7 cut(s) 241, 391, 466, 518, 722, 890, 958
TaiI ACGT 2 cut(s) 197, 452
TaqI TCGA 3 cut(s) 510, 565, 897
TasI AATT 4 cut(s) 229, 336, 393, 962
TatI WGTACW 1 cut(s) 49
TfiI GAWTC 1 cut(s) 373
TscAI CASTG 2 cut(s) 523, 534
TseFI GTSAC 2 cut(s) 87, 705
TseI GCWGC 3 cut(s) 32, 524, 761
Tsp45I GTSAC 2 cut(s) 87, 705
TspDTI ATGAA 4 cut(s) 192, 676, 801, 1060
TspGWI ACGGA 1 cut(s) 187
TspRI CASTG 2 cut(s) 523, 534
XapI RAATTY 2 cut(s) 229, 962
XbaI TCTAGA 1 cut(s) 170
XmnI GAANNNNTTC 1 cut(s) 1028
XspI CTAG 2 cut(s) 171, 443
ZraI GACGTC 1 cut(s) 195
ZrmI AGTACT 1 cut(s) 51
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.