Rw5G033480

Pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0013350
Physical Location & Seq
Reverse (-)
57011307 .. 57013238
1932 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G033480.1

Sequence Viewer

Length: 1104 bp
ATGGAGTTGGGTGTTTTTAGTGCTTCTGTTGCTGCTGGTGCTGGTTTAGTACTACTGGTTTTGTTGGGTGGTTTTCAGTTGGGTTTCTCACAAAGTGAAGCATATGCAAGGAGTTATATCAGCTGGGATGATTTGAAGGTGGATGAGCAGATGGTCATTGGTTTGAACTCTGGAAATGAAGAGCAAAACGGGACGCGCGTCATTGTGGTGGACAAGAATGGAGAAGGAAACTCTATGACAATTCAAGCAGCAGTTGACATGGTTCCACACCAAACACCCAAAGAGTCAAAATTTACATTCTTCCAGGAATTTATAGGTCAAGAGAAGGTGCTTATACCAATTACCAAGCCATACATATCATTTATAGGGGACCAGAATCGAACTTCTGAGACTGTAATTACTTGGAACAGTAAGGCATCTGATAAGGACAGCAGTGGCTTTGAACTAGGAACGTACAGAACAGCAACTGTAGCCATAGAGGCTGATTACTTTTGTGCAACAGGGATCACTTTCGAGAACAGTGCGGTTGCAGTGCCTGGAGGATATGGAATGCAAGGAGTGGCTCTTAGAGTTGCTGGGGATAAGGCCATGTTCTATAGAGTACGGGTTTTGGGAGCACAAGATACATTATTGGATGAGAATGGATCGCACTACTTCTACGAATCCCACATTCAAGGAAGTGTGGACTTCATATTTGGAAAATCAAGGTCACTCTACCAGGACTGTGTTATCCAATCAACTGCTAAGACCTACGGAGCAATTGCAGCTCATCATAGAGACTCACCAAATGAAGATACAGGGTTCTCTTTCGTGAACTGCAAAATCATCGGAACTGGAGATATCTACTTGGGAAGAGCTTGGGGAGCTTATTCAAGAGTCATATATTCCTTTTGTCATATTGACAATATAATAACTCCTCTGGGATGGAGTGACTGGAACATGCCACCGAGGCAGAGGACTGTGGAATTCGGAGAATACCAATGCAGAGGCAAAGGAGCAGATAGAAGAGGCAGGGTACCATGGTTGAAGTCTTTTAGTCCTGACGAGATAACCCCTTTTCTGGATACCAAATTCATATATGGAGAGCAATGGCTTAGACTATAG

Protein Analysis

367

Amino Acids

40.71

Weight (kDa)

5.26

Isoelectric Point (pI)

30.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 68 - 361 9.7e-59 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013350)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G55590 AT5G55590
fragaria_vesca FvH4_3g29490
malus_domestica MD03G1186900.v1.1 MD11G1201900.v1.1
prunus_persica Prupe.4G239700_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0054371
rosa_laevigata RLG00000034927
rosa_multiflora Rmu_sc0005705.1_g000023 Rmu_sc0023043.1_g000001
rosa_roxburghii Rroxscaffold_1G00026500
rosa_rugosa Rorug05G0285900
rosa_samantha Rh5AG356400 Rh5BG368000 Rh5CG391300 Rh5DG382200
rosa_wichuraiana Rw5G033480 Rw5G033650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1015
AccB1I GGYRCC 1 cut(s) 1015
AccII CGCG 2 cut(s) 196, 198
AciI CCGC 1 cut(s) 524
AclWI GGATC 2 cut(s) 512, 652
AcsI RAATTY 4 cut(s) 290, 308, 965, 1070
AdeI CACNNNGTG 1 cut(s) 95
AfaI GTAC 4 cut(s) 51, 455, 603, 1017
AfiI CCNNNNNNNGG 1 cut(s) 1060
AgsI TTSAA 7 cut(s) 136, 166, 245, 443, 674, 873, 1027
AjnI CCWGG 3 cut(s) 303, 535, 717
AluBI AGCT 4 cut(s) 123, 767, 857, 866
AluI AGCT 4 cut(s) 123, 767, 857, 866
Alw21I GWGCWC 1 cut(s) 619
Alw26I GTCTC 2 cut(s) 383, 771
AlwI GGATC 2 cut(s) 512, 652
AlwNI CAGNNNCTG 2 cut(s) 467, 536
AoxI GGCC 1 cut(s) 585
ApeKI GCWGC 3 cut(s) 32, 248, 764
ApoI RAATTY 4 cut(s) 290, 308, 965, 1070
ArsI GACNNNNNNTTYG 2 cut(s) 677, 709
Asp718I GGTACC 1 cut(s) 1015
AspLEI GCGC 1 cut(s) 198
AspS9I GGNCC 1 cut(s) 370
AsuHPI GGTGA 1 cut(s) 774
AvaII GGWCC 1 cut(s) 370
BaeI ACNNNNGTAYC 2 cut(s) 1007, 1040
BanI GGYRCC 1 cut(s) 1015
Bbv12I GWGCWC 1 cut(s) 619
BbvI GCAGC 3 cut(s) 19, 260, 776
BccI CCATC 2 cut(s) 145, 918
BcgI CGANNNNNNTGC 4 cut(s) 503, 537, 808, 842
BciT130I CCWGG 3 cut(s) 305, 537, 719
BciVI GTATCC 1 cut(s) 1057
BcoDI GTCTC 2 cut(s) 383, 771
BfaI CTAG 1 cut(s) 446
BfmI CTRYAG 3 cut(s) 468, 595, 1100
BfuI GTATCC 1 cut(s) 1057
BglI GCCNNNNNGGC 2 cut(s) 479, 949
BisI GCNGC 3 cut(s) 33, 249, 765
BlsI GCNGC 3 cut(s) 34, 250, 766
BmcAI AGTACT 1 cut(s) 51
Bme1390I CCNGG 3 cut(s) 305, 537, 719
Bme18I GGWCC 1 cut(s) 370
BmgT120I GGNCC 1 cut(s) 370
BmiI GGNNCC 3 cut(s) 264, 371, 1017
BmrFI CCNGG 3 cut(s) 305, 537, 719
BmsI GCATC 1 cut(s) 425
BoxI GACNNNNGTC 1 cut(s) 197
BpmI CTGGAG 2 cut(s) 558, 855
BsaJI CCNNGG 2 cut(s) 947, 1019
Bsc4I CCNNNNNNNGG 1 cut(s) 1060
Bse1I ACTGG 3 cut(s) 60, 838, 938
Bse3DI GCAATG 1 cut(s) 1094
BseBI CCWGG 3 cut(s) 305, 537, 719
BseDI CCNNGG 2 cut(s) 947, 1019
BseGI GGATG 4 cut(s) 133, 148, 640, 929
BseLI CCNNNNNNNGG 1 cut(s) 1060
BseMI GCAATG 1 cut(s) 1094
BseMII CTCAG 1 cut(s) 378
BseNI ACTGG 3 cut(s) 60, 838, 938
BseRI GAGGAG 1 cut(s) 906
BseXI GCAGC 3 cut(s) 19, 260, 776
BseYI CCCAGC 2 cut(s) 123, 575
Bsh1236I CGCG 2 cut(s) 196, 198
BshFI GGCC 1 cut(s) 587
BshNI GGYRCC 1 cut(s) 1015
BsiHKAI GWGCWC 1 cut(s) 619
BslFI GGGAC 2 cut(s) 205, 383
BslI CCNNNNNNNGG 1 cut(s) 1060
BsmAI GTCTC 2 cut(s) 383, 771
BsmFI GGGAC 2 cut(s) 205, 383
BsmI GAATGC 1 cut(s) 555
BsnI GGCC 1 cut(s) 587
Bsp1286I GDGCHC 1 cut(s) 619
Bsp143I GATC 2 cut(s) 504, 644
Bsp19I CCATGG 1 cut(s) 1019
BspACI CCGC 1 cut(s) 524
BspANI GGCC 1 cut(s) 587
BspCNI CTCAG 1 cut(s) 379
BspFNI CGCG 2 cut(s) 196, 198
BspLI GGNNCC 3 cut(s) 264, 371, 1017
BspPI GGATC 2 cut(s) 512, 652
BspQI GCTCTTC 2 cut(s) 174, 847
BspT107I GGYRCC 1 cut(s) 1015
BsrDI GCAATG 1 cut(s) 1094
BsrI ACTGG 3 cut(s) 60, 838, 938
BssECI CCNNGG 2 cut(s) 947, 1019
BssMI GATC 2 cut(s) 504, 644
BssT1I CCWWGG 1 cut(s) 1019
Bst2UI CCWGG 3 cut(s) 305, 537, 719
Bst4CI ACNGT 6 cut(s) 394, 410, 469, 521, 725, 961
Bst6I CTCTTC 3 cut(s) 174, 847, 1000
BstDEI CTNAG 4 cut(s) 387, 566, 744, 1094
BstDSI CCRYGG 1 cut(s) 1019
BstF5I GGATG 4 cut(s) 133, 148, 640, 929
BstFNI CGCG 2 cut(s) 196, 198
BstHHI GCGC 1 cut(s) 198
BstKTI GATC 2 cut(s) 507, 647
BstMAI GTCTC 2 cut(s) 383, 771
BstMBI GATC 2 cut(s) 504, 644
BstMWI GCNNNNNNNGC 7 cut(s) 29, 38, 470, 479, 764, 863, 949
BstNI CCWGG 3 cut(s) 305, 537, 719
BstNSI RCATGY 1 cut(s) 943
BstPAI GACNNNNGTC 1 cut(s) 197
BstSCI CCNGG 3 cut(s) 303, 535, 717
BstSFI CTRYAG 3 cut(s) 468, 595, 1100
BstUI CGCG 2 cut(s) 196, 198
BstV1I GCAGC 3 cut(s) 19, 260, 776
BsuI GTATCC 1 cut(s) 1057
BsuRI GGCC 1 cut(s) 587
BtgI CCRYGG 1 cut(s) 1019
BtsCI GGATG 4 cut(s) 133, 148, 640, 929
BtsI GCAGTG 2 cut(s) 439, 537
BtsIMutI CAGTG 3 cut(s) 439, 526, 537
CaiI CAGNNNCTG 2 cut(s) 467, 536
CfoI GCGC 1 cut(s) 198
Cfr13I GGNCC 1 cut(s) 370
CseI GACGC 2 cut(s) 187, 202
Csp6I GTAC 4 cut(s) 50, 454, 602, 1016
CviAII CATG 4 cut(s) 259, 589, 940, 1020
CviQI GTAC 4 cut(s) 50, 454, 602, 1016
DdeI CTNAG 4 cut(s) 387, 566, 744, 1094
DpnI GATC 2 cut(s) 506, 646
DpnII GATC 2 cut(s) 504, 644
DraIII CACNNNGTG 1 cut(s) 95
Eam1104I CTCTTC 3 cut(s) 174, 847, 1000
EarI CTCTTC 3 cut(s) 174, 847, 1000
Eco130I CCWWGG 1 cut(s) 1019
Eco32I GATATC 1 cut(s) 841
Eco47I GGWCC 1 cut(s) 370
EcoRI GAATTC 1 cut(s) 965
EcoRII CCWGG 3 cut(s) 303, 535, 717
EcoRV GATATC 1 cut(s) 841
EcoT14I CCWWGG 1 cut(s) 1019
ErhI CCWWGG 1 cut(s) 1019
FaeI CATG 4 cut(s) 262, 592, 943, 1023
FaqI GGGAC 2 cut(s) 205, 383
FatI CATG 4 cut(s) 258, 588, 939, 1019
FauNDI CATATG 1 cut(s) 103
Fnu4HI GCNGC 3 cut(s) 33, 249, 765
FokI GGATG 4 cut(s) 140, 155, 647, 936
Fsp4HI GCNGC 3 cut(s) 33, 249, 765
FspBI CTAG 1 cut(s) 446
GlaI GCGC 1 cut(s) 197
GluI GCNGC 3 cut(s) 33, 249, 765
GsaI CCCAGC 2 cut(s) 127, 579
GsuI CTGGAG 2 cut(s) 558, 855
HaeIII GGCC 1 cut(s) 587
HgaI GACGC 2 cut(s) 187, 202
HhaI GCGC 1 cut(s) 198
Hin1II CATG 4 cut(s) 262, 592, 943, 1023
Hin6I GCGC 1 cut(s) 196
HinP1I GCGC 1 cut(s) 196
HincII GTYRAC 1 cut(s) 256
HindII GTYRAC 1 cut(s) 256
HinfI GANTC 5 cut(s) 284, 376, 662, 779, 876
HphI GGTGA 1 cut(s) 774
Hpy166II GTNNAC 4 cut(s) 211, 256, 685, 814
Hpy188I TCNGA 4 cut(s) 388, 421, 830, 971
Hpy188III TCNNGA 7 cut(s) 171, 320, 514, 811, 873, 1040, 1061
Hpy8I GTNNAC 4 cut(s) 211, 256, 685, 814
HpyAV CCTTC 3 cut(s) 130, 218, 319
HpyCH4III ACNGT 6 cut(s) 394, 410, 469, 521, 725, 961
HpyCH4IV ACGT 1 cut(s) 452
HpyCH4V TGCA 7 cut(s) 107, 497, 530, 553, 764, 819, 984
HpyF10VI GCNNNNNNNGC 7 cut(s) 29, 38, 470, 479, 764, 863, 949
HpyF3I CTNAG 4 cut(s) 387, 566, 744, 1094
HpySE526I ACGT 1 cut(s) 452
Hsp92II CATG 4 cut(s) 262, 592, 943, 1023
HspAI GCGC 1 cut(s) 196
KpnI GGTACC 1 cut(s) 1019
Kzo9I GATC 2 cut(s) 504, 644
LguI GCTCTTC 2 cut(s) 174, 847
LmnI GCTCC 4 cut(s) 614, 755, 863, 995
Lsp1109I GCAGC 3 cut(s) 19, 260, 776
LweI GCATC 1 cut(s) 425
MaeI CTAG 1 cut(s) 446
MaeII ACGT 1 cut(s) 452
MaeIII GTNAC 2 cut(s) 708, 929
MalI GATC 2 cut(s) 506, 646
MboI GATC 2 cut(s) 504, 644
MboII GAAGA 5 cut(s) 191, 292, 803, 864, 1017
MfeI CAATTG 1 cut(s) 759
MhlI GDGCHC 1 cut(s) 619
MluCI AATT 8 cut(s) 240, 290, 308, 339, 396, 759, 965, 1070
MlyI GAGTC 3 cut(s) 293, 773, 885
MnlI CCTC 7 cut(s) 472, 533, 927, 942, 948, 980, 1001
MslI CAYNNNNRTG 1 cut(s) 206
MspA1I CMGCKG 1 cut(s) 123
MspR9I CCNGG 3 cut(s) 305, 537, 719
MunI CAATTG 1 cut(s) 759
Mva1269I GAATGC 1 cut(s) 555
MvaI CCWGG 3 cut(s) 305, 537, 719
MvnI CGCG 2 cut(s) 196, 198
MwoI GCNNNNNNNGC 7 cut(s) 29, 38, 470, 479, 764, 863, 949
NcoI CCATGG 1 cut(s) 1019
NdeI CATATG 1 cut(s) 103
NdeII GATC 2 cut(s) 504, 644
NlaIII CATG 4 cut(s) 262, 592, 943, 1023
NlaIV GGNNCC 3 cut(s) 264, 371, 1017
NmuCI GTSAC 2 cut(s) 708, 929
NspI RCATGY 1 cut(s) 943
PciSI GCTCTTC 2 cut(s) 174, 847
PcsI WCGNNNNNNNCGW 1 cut(s) 195
PctI GAATGC 1 cut(s) 555
PfeI GAWTC 2 cut(s) 376, 662
PfoI TCCNGGA 1 cut(s) 303
PkrI GCNGC 3 cut(s) 34, 250, 766
PleI GAGTC 3 cut(s) 292, 773, 884
PpsI GAGTC 3 cut(s) 292, 773, 884
PshAI GACNNNNGTC 1 cut(s) 197
Psp6I CCWGG 3 cut(s) 303, 535, 717
PspFI CCCAGC 2 cut(s) 123, 575
PspGI CCWGG 3 cut(s) 303, 535, 717
PspN4I GGNNCC 3 cut(s) 264, 371, 1017
PspPI GGNCC 1 cut(s) 370
PstNI CAGNNNCTG 2 cut(s) 467, 536
PvuII CAGCTG 1 cut(s) 123
RsaI GTAC 4 cut(s) 51, 455, 603, 1017
RsaNI GTAC 4 cut(s) 50, 454, 602, 1016
RseI CAYNNNNRTG 1 cut(s) 206
SapI GCTCTTC 2 cut(s) 174, 847
SatI GCNGC 3 cut(s) 33, 249, 765
Sau3AI GATC 2 cut(s) 504, 644
Sau96I GGNCC 1 cut(s) 370
ScaI AGTACT 1 cut(s) 51
SchI GAGTC 3 cut(s) 293, 773, 885
ScrFI CCNGG 3 cut(s) 305, 537, 719
SduI GDGCHC 1 cut(s) 619
SfaNI GCATC 1 cut(s) 425
SfcI CTRYAG 3 cut(s) 468, 595, 1100
SinI GGWCC 1 cut(s) 370
SmiMI CAYNNNNRTG 1 cut(s) 206
Sse9I AATT 8 cut(s) 240, 290, 308, 339, 396, 759, 965, 1070
SsiI CCGC 1 cut(s) 524
SspMI CTAG 1 cut(s) 446
StyD4I CCNGG 3 cut(s) 303, 535, 717
StyI CCWWGG 1 cut(s) 1019
TaaI ACNGT 6 cut(s) 394, 410, 469, 521, 725, 961
TaiI ACGT 1 cut(s) 455
TaqI TCGA 2 cut(s) 379, 513
TasI AATT 8 cut(s) 240, 290, 308, 339, 396, 759, 965, 1070
TatI WGTACW 1 cut(s) 49
TfiI GAWTC 2 cut(s) 376, 662
TscAI CASTG 3 cut(s) 439, 526, 537
TseFI GTSAC 2 cut(s) 708, 929
TseI GCWGC 3 cut(s) 32, 248, 764
Tsp45I GTSAC 2 cut(s) 708, 929
TspDTI ATGAA 4 cut(s) 192, 679, 804, 1063
TspGWI ACGGA 1 cut(s) 768
TspRI CASTG 3 cut(s) 439, 526, 537
VpaK11BI GGWCC 1 cut(s) 370
XapI RAATTY 4 cut(s) 290, 308, 965, 1070
XceI RCATGY 1 cut(s) 943
XspI CTAG 1 cut(s) 446
ZrmI AGTACT 1 cut(s) 51
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.