Rroxscaffold_1G00026500

Pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0013350
Physical Location & Seq
Forward (+)
33379952 .. 33381596
1645 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00026500.1

Sequence Viewer

Length: 1062 bp
ATGGAGTTGGGTGTTTTTAGTGCTTCTGTTGCCGCTGGTGCTGGTTTAGTACTACTGGTTTTGTTGGGTGGTTTTCAGTTGGGTTTCTCACAAAGTGAAGCATATGCAGGGAGTTATATCAGCTGGGATGATTTGAAGGTGGATGAGCAGGTGGTCATTGGTTTGAACTCTGGAAATGAAGAGCAAAACGGGACGCGCGTCATTGTGGTGGACAAGAATGGAGAAGGAAACTCTATGACAGTTCAAGCAGCAGTTGACATGGTTCCACACCATAACACCCAAAGAGTCAAAATTTACATTCTTCCAGGAATTTATAGAGAGAAGGTGCTTATACCAATTACCAAGCCATACATATCATTTATAGGGGACCAGAATCGAACTTCTGAGACTGTAATTACTTGGAACAATAAGGCATCTGATAAGGACAGCAATGGCTTTGAACTAGGAACGTACAGAACAGCAACTGTAGCCATAGAGGCTGATTACTTTTGTGCAACAGGGATCACTTTCGAGAACAGTGTGGATGCAGTGCCTGGAGGATATGGAATGCAAGGAGTGGCTCTTAGAGTTGCTGGGGATAAGGCCATGTTCTATAGAGCAAGGGTTTTGGGAGCACAAGATACATTATTGGATGAGAATGGATCGCACTACTTCTACGAATCCCACATTCAAGGAAGTGTGGACTTCATATTTGGAAAATCAAGGTCACTCTACCAGGACTGTGTTATCCAATCAACTGCTAAGACCTATGGAGCAATTGCAGCTCATCATAGAGACTCACCAAATGAAGATACAGGGTTCTCTTTCGTGAACTGCAAAATCATCGGAACTGGAGATATCTACTTGGGAAGAGCTTGGGGAGCTTATTCAAGAGTCATATATTCCTATTGTCATATTGACAATATAATAACTCCTCTGGGATGGAGTGACTGGAACATGCCACCAAGGCAGAGGTATCCTTCAAGCTTGAACATTAGGCATCTTGAATTTATCTCCAAGCAGAGCACTCAAATCACATTATTGCTCCCTCTAATAGGCCAGAAGCTTTTGTGGGAGTTCTAA

Protein Analysis

353

Amino Acids

38.99

Weight (kDa)

5.5

Isoelectric Point (pI)

30.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pectinesterase PF01095 68 - 313 1.9e-56 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013350)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G55590 AT5G55590
fragaria_vesca FvH4_3g29490
malus_domestica MD03G1186900.v1.1 MD11G1201900.v1.1
prunus_persica Prupe.4G239700_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0054371
rosa_laevigata RLG00000034927
rosa_multiflora Rmu_sc0005705.1_g000023 Rmu_sc0023043.1_g000001
rosa_roxburghii Rroxscaffold_1G00026500
rosa_rugosa Rorug05G0285900
rosa_samantha Rh5AG356400 Rh5BG368000 Rh5CG391300 Rh5DG382200
rosa_wichuraiana Rw5G033480 Rw5G033650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 139
Acc36I ACCTGC 1 cut(s) 139
AccII CGCG 2 cut(s) 196, 198
AciI CCGC 1 cut(s) 33
AclWI GGATC 2 cut(s) 509, 649
AcsI RAATTY 3 cut(s) 291, 309, 986
AdeI CACNNNGTG 1 cut(s) 95
AfaI GTAC 2 cut(s) 51, 452
AfiI CCNNNNNNNGG 1 cut(s) 1034
AgsI TTSAA 9 cut(s) 136, 166, 245, 440, 671, 870, 963, 970, 986
AjnI CCWGG 3 cut(s) 304, 532, 714
AluBI AGCT 6 cut(s) 123, 764, 854, 863, 966, 1045
AluI AGCT 6 cut(s) 123, 764, 854, 863, 966, 1045
Alw21I GWGCWC 2 cut(s) 616, 1007
Alw26I GTCTC 2 cut(s) 380, 768
AlwI GGATC 2 cut(s) 509, 649
AlwNI CAGNNNCTG 2 cut(s) 464, 533
AoxI GGCC 2 cut(s) 582, 1036
ApeKI GCWGC 2 cut(s) 248, 761
ApoI RAATTY 3 cut(s) 291, 309, 986
ArsI GACNNNNNNTTYG 2 cut(s) 674, 706
AspLEI GCGC 1 cut(s) 198
AspS9I GGNCC 1 cut(s) 367
AsuHPI GGTGA 1 cut(s) 771
AvaII GGWCC 1 cut(s) 367
Bbv12I GWGCWC 2 cut(s) 616, 1007
BbvI GCAGC 2 cut(s) 260, 773
BccI CCATC 1 cut(s) 915
BcgI CGANNNNNNTGC 2 cut(s) 805, 839
BciT130I CCWGG 3 cut(s) 306, 534, 716
BciVI GTATCC 1 cut(s) 966
BcoDI GTCTC 2 cut(s) 380, 768
BfaI CTAG 1 cut(s) 443
BfmI CTRYAG 2 cut(s) 465, 592
BfuAI ACCTGC 1 cut(s) 139
BfuI GTATCC 1 cut(s) 966
BglI GCCNNNNNGGC 2 cut(s) 476, 946
BisI GCNGC 3 cut(s) 33, 249, 762
BlsI GCNGC 3 cut(s) 34, 250, 763
BmcAI AGTACT 1 cut(s) 51
Bme1390I CCNGG 3 cut(s) 306, 534, 716
Bme18I GGWCC 1 cut(s) 367
BmgT120I GGNCC 1 cut(s) 367
BmiI GGNNCC 2 cut(s) 264, 368
BmrFI CCNGG 3 cut(s) 306, 534, 716
BmsI GCATC 3 cut(s) 422, 514, 988
BoxI GACNNNNGTC 1 cut(s) 197
BpmI CTGGAG 2 cut(s) 555, 852
BsaJI CCNNGG 1 cut(s) 944
Bsc4I CCNNNNNNNGG 1 cut(s) 1034
Bse1I ACTGG 3 cut(s) 60, 835, 935
Bse3DI GCAATG 1 cut(s) 436
BseBI CCWGG 3 cut(s) 306, 534, 716
BseDI CCNNGG 1 cut(s) 944
BseGI GGATG 5 cut(s) 133, 148, 529, 637, 926
BseLI CCNNNNNNNGG 1 cut(s) 1034
BseMI GCAATG 1 cut(s) 436
BseMII CTCAG 1 cut(s) 375
BseNI ACTGG 3 cut(s) 60, 835, 935
BseRI GAGGAG 1 cut(s) 903
BseXI GCAGC 2 cut(s) 260, 773
BseYI CCCAGC 2 cut(s) 123, 572
Bsh1236I CGCG 2 cut(s) 196, 198
BshFI GGCC 2 cut(s) 584, 1038
BsiHKAI GWGCWC 2 cut(s) 616, 1007
BslFI GGGAC 2 cut(s) 205, 380
BslI CCNNNNNNNGG 1 cut(s) 1034
BsmAI GTCTC 2 cut(s) 380, 768
BsmFI GGGAC 2 cut(s) 205, 380
BsmI GAATGC 1 cut(s) 552
BsnI GGCC 2 cut(s) 584, 1038
Bsp1286I GDGCHC 2 cut(s) 616, 1007
Bsp143I GATC 2 cut(s) 501, 641
BspACI CCGC 1 cut(s) 33
BspANI GGCC 2 cut(s) 584, 1038
BspCNI CTCAG 1 cut(s) 376
BspFNI CGCG 2 cut(s) 196, 198
BspLI GGNNCC 2 cut(s) 264, 368
BspMI ACCTGC 1 cut(s) 139
BspPI GGATC 2 cut(s) 509, 649
BspQI GCTCTTC 2 cut(s) 174, 844
BsrDI GCAATG 1 cut(s) 436
BsrI ACTGG 3 cut(s) 60, 835, 935
BssECI CCNNGG 1 cut(s) 944
BssMI GATC 2 cut(s) 501, 641
BssT1I CCWWGG 1 cut(s) 944
Bst2UI CCWGG 3 cut(s) 306, 534, 716
Bst4CI ACNGT 5 cut(s) 241, 391, 466, 518, 722
Bst6I CTCTTC 2 cut(s) 174, 844
BstDEI CTNAG 3 cut(s) 384, 563, 741
BstENI CCTNNNNNAGG 1 cut(s) 1032
BstF5I GGATG 5 cut(s) 133, 148, 529, 637, 926
BstFNI CGCG 2 cut(s) 196, 198
BstHHI GCGC 1 cut(s) 198
BstKTI GATC 2 cut(s) 504, 644
BstMAI GTCTC 2 cut(s) 380, 768
BstMBI GATC 2 cut(s) 501, 641
BstMWI GCNNNNNNNGC 7 cut(s) 29, 38, 467, 476, 761, 860, 946
BstNI CCWGG 3 cut(s) 306, 534, 716
BstNSI RCATGY 1 cut(s) 940
BstPAI GACNNNNGTC 1 cut(s) 197
BstSCI CCNGG 3 cut(s) 304, 532, 714
BstSFI CTRYAG 2 cut(s) 465, 592
BstUI CGCG 2 cut(s) 196, 198
BstV1I GCAGC 2 cut(s) 260, 773
BsuI GTATCC 1 cut(s) 966
BsuRI GGCC 2 cut(s) 584, 1038
BtsCI GGATG 5 cut(s) 133, 148, 529, 637, 926
BtsI GCAGTG 1 cut(s) 534
BtsIMutI CAGTG 2 cut(s) 523, 534
BveI ACCTGC 1 cut(s) 139
CaiI CAGNNNCTG 2 cut(s) 464, 533
CfoI GCGC 1 cut(s) 198
Cfr13I GGNCC 1 cut(s) 367
CseI GACGC 2 cut(s) 187, 202
Csp6I GTAC 2 cut(s) 50, 451
CviAII CATG 3 cut(s) 259, 586, 937
CviQI GTAC 2 cut(s) 50, 451
DdeI CTNAG 3 cut(s) 384, 563, 741
DpnI GATC 2 cut(s) 503, 643
DpnII GATC 2 cut(s) 501, 641
DraIII CACNNNGTG 1 cut(s) 95
Eam1104I CTCTTC 2 cut(s) 174, 844
EarI CTCTTC 2 cut(s) 174, 844
Eco130I CCWWGG 1 cut(s) 944
Eco32I GATATC 1 cut(s) 838
Eco47I GGWCC 1 cut(s) 367
EcoNI CCTNNNNNAGG 1 cut(s) 1032
EcoRII CCWGG 3 cut(s) 304, 532, 714
EcoRV GATATC 1 cut(s) 838
EcoT14I CCWWGG 1 cut(s) 944
ErhI CCWWGG 1 cut(s) 944
FaeI CATG 3 cut(s) 262, 589, 940
FaqI GGGAC 2 cut(s) 205, 380
FatI CATG 3 cut(s) 258, 585, 936
FauNDI CATATG 1 cut(s) 103
Fnu4HI GCNGC 3 cut(s) 33, 249, 762
FokI GGATG 5 cut(s) 140, 155, 536, 644, 933
Fsp4HI GCNGC 3 cut(s) 33, 249, 762
FspBI CTAG 1 cut(s) 443
GlaI GCGC 1 cut(s) 197
GluI GCNGC 3 cut(s) 33, 249, 762
GsaI CCCAGC 2 cut(s) 127, 576
GsuI CTGGAG 2 cut(s) 555, 852
HaeIII GGCC 2 cut(s) 584, 1038
HgaI GACGC 2 cut(s) 187, 202
HhaI GCGC 1 cut(s) 198
Hin1II CATG 3 cut(s) 262, 589, 940
Hin6I GCGC 1 cut(s) 196
HinP1I GCGC 1 cut(s) 196
HincII GTYRAC 1 cut(s) 256
HindII GTYRAC 1 cut(s) 256
HindIII AAGCTT 2 cut(s) 964, 1043
HinfI GANTC 5 cut(s) 285, 373, 659, 776, 873
HphI GGTGA 1 cut(s) 771
Hpy166II GTNNAC 4 cut(s) 211, 256, 682, 811
Hpy188I TCNGA 3 cut(s) 385, 418, 827
Hpy188III TCNNGA 5 cut(s) 171, 511, 808, 870, 983
Hpy8I GTNNAC 4 cut(s) 211, 256, 682, 811
HpyAV CCTTC 4 cut(s) 130, 218, 316, 969
HpyCH4III ACNGT 5 cut(s) 241, 391, 466, 518, 722
HpyCH4IV ACGT 1 cut(s) 449
HpyCH4V TGCA 6 cut(s) 107, 494, 527, 550, 761, 816
HpyF10VI GCNNNNNNNGC 7 cut(s) 29, 38, 467, 476, 761, 860, 946
HpyF3I CTNAG 3 cut(s) 384, 563, 741
HpySE526I ACGT 1 cut(s) 449
Hsp92II CATG 3 cut(s) 262, 589, 940
HspAI GCGC 1 cut(s) 196
Kzo9I GATC 2 cut(s) 501, 641
LguI GCTCTTC 2 cut(s) 174, 844
LmnI GCTCC 4 cut(s) 611, 752, 860, 1029
Lsp1109I GCAGC 2 cut(s) 260, 773
LweI GCATC 3 cut(s) 422, 514, 988
MaeI CTAG 1 cut(s) 443
MaeII ACGT 1 cut(s) 449
MaeIII GTNAC 2 cut(s) 705, 926
MalI GATC 2 cut(s) 503, 643
MboI GATC 2 cut(s) 501, 641
MboII GAAGA 4 cut(s) 191, 293, 800, 861
MfeI CAATTG 1 cut(s) 756
MhlI GDGCHC 2 cut(s) 616, 1007
MluCI AATT 6 cut(s) 291, 309, 336, 393, 756, 986
MlyI GAGTC 3 cut(s) 294, 770, 882
MnlI CCTC 5 cut(s) 469, 530, 924, 945, 1038
MslI CAYNNNNRTG 1 cut(s) 206
MspA1I CMGCKG 2 cut(s) 35, 123
MspR9I CCNGG 3 cut(s) 306, 534, 716
MunI CAATTG 1 cut(s) 756
Mva1269I GAATGC 1 cut(s) 552
MvaI CCWGG 3 cut(s) 306, 534, 716
MvnI CGCG 2 cut(s) 196, 198
MwoI GCNNNNNNNGC 7 cut(s) 29, 38, 467, 476, 761, 860, 946
NdeI CATATG 1 cut(s) 103
NdeII GATC 2 cut(s) 501, 641
NlaIII CATG 3 cut(s) 262, 589, 940
NlaIV GGNNCC 2 cut(s) 264, 368
NmuCI GTSAC 2 cut(s) 705, 926
NspI RCATGY 1 cut(s) 940
PaqCI CACCTGC 1 cut(s) 139
PciSI GCTCTTC 2 cut(s) 174, 844
PcsI WCGNNNNNNNCGW 1 cut(s) 195
PctI GAATGC 1 cut(s) 552
PfeI GAWTC 2 cut(s) 373, 659
PfoI TCCNGGA 1 cut(s) 304
PkrI GCNGC 3 cut(s) 34, 250, 763
PleI GAGTC 3 cut(s) 293, 770, 881
PpsI GAGTC 3 cut(s) 293, 770, 881
PshAI GACNNNNGTC 1 cut(s) 197
Psp6I CCWGG 3 cut(s) 304, 532, 714
PspFI CCCAGC 2 cut(s) 123, 572
PspGI CCWGG 3 cut(s) 304, 532, 714
PspN4I GGNNCC 2 cut(s) 264, 368
PspPI GGNCC 1 cut(s) 367
PstNI CAGNNNCTG 2 cut(s) 464, 533
PvuII CAGCTG 1 cut(s) 123
RsaI GTAC 2 cut(s) 51, 452
RsaNI GTAC 2 cut(s) 50, 451
RseI CAYNNNNRTG 1 cut(s) 206
SapI GCTCTTC 2 cut(s) 174, 844
SatI GCNGC 3 cut(s) 33, 249, 762
Sau3AI GATC 2 cut(s) 501, 641
Sau96I GGNCC 1 cut(s) 367
ScaI AGTACT 1 cut(s) 51
SchI GAGTC 3 cut(s) 294, 770, 882
ScrFI CCNGG 3 cut(s) 306, 534, 716
SduI GDGCHC 2 cut(s) 616, 1007
SfaNI GCATC 3 cut(s) 422, 514, 988
SfcI CTRYAG 2 cut(s) 465, 592
SinI GGWCC 1 cut(s) 367
SmiMI CAYNNNNRTG 1 cut(s) 206
Sse9I AATT 6 cut(s) 291, 309, 336, 393, 756, 986
SsiI CCGC 1 cut(s) 33
SspMI CTAG 1 cut(s) 443
StyD4I CCNGG 3 cut(s) 304, 532, 714
StyI CCWWGG 1 cut(s) 944
TaaI ACNGT 5 cut(s) 241, 391, 466, 518, 722
TaiI ACGT 1 cut(s) 452
TaqI TCGA 2 cut(s) 376, 510
TasI AATT 6 cut(s) 291, 309, 336, 393, 756, 986
TatI WGTACW 1 cut(s) 49
TauI GCSGC 1 cut(s) 35
TfiI GAWTC 2 cut(s) 373, 659
TscAI CASTG 2 cut(s) 523, 534
TseFI GTSAC 2 cut(s) 705, 926
TseI GCWGC 2 cut(s) 248, 761
Tsp45I GTSAC 2 cut(s) 705, 926
TspDTI ATGAA 3 cut(s) 192, 676, 801
TspRI CASTG 2 cut(s) 523, 534
VpaK11BI GGWCC 1 cut(s) 367
XagI CCTNNNNNAGG 1 cut(s) 1032
XapI RAATTY 3 cut(s) 291, 309, 986
XceI RCATGY 1 cut(s) 940
XspI CTAG 1 cut(s) 443
ZrmI AGTACT 1 cut(s) 51
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.