AT5G56530

Neprosin activation peptide

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0004991
Physical Location & Seq
Forward (+)
22889073 .. 22891866
2794 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G56530.2

Sequence Viewer

Length: 1263 bp
ATGGCTGCGGCGCATTTTAGTAAGGAGAGGGTCTTCAGAGGGTTTCTGGTTTGGTTCTGCTTTTGGGGACTGATGTCTCTAACATGCGCTGGGAGACTCAGTGTTTCAAGGCAGAATTTTGAAGTGCACAAACACTTGAATCGATTGAATAAACCAGCCGTTAAGAGCATTCAGAGTCCAGATGGTGACATAATAGACTGTGTTCACATATCGAAGCAACCAGCTTTTGATCACCCTTTCCTCAAAGATCACAAGATTCAGATGGGACCTAGTTACACTCCAGAATCGCTGTTTGGTGAGAGCAAAGTGTCTGAGAAACCAAAAGAGAGTGTCAATCCTATTACACAGTTATGGCACCAGAACGGAGTTTGTTCTGAAGGGACAATACCAGTGAGGAGGACAAAGAAAGAGGATGTCTTGAGGGCGAGTTCTGTTAAACGGTATGGTAAGAAGAAGCACCTTTCTGTTCCTCTGCCTCGATCTGCTGATCCTGATCTCATTAACCAAAGTGGTCATCAGCACGCTATAGCTTATGTGGAAGGAGGCAAGTTTTACGGGGCAAAGGCGACAATAAATGTATGGGAACCCAAAGTGCAAAGCTCTAATGAGTTTAGCTTGTCTCAGTTATGGATTCTTGGAGGTTCTTTTGGTCAAGATCTCAACAGCATTGAAGCTGGTTGGCAGGTTAGTCCAGATTTATATGGCGATAACAACACAAGGCTTTTCACATACTGGACGAGCGATGCTTATCAAGCTACGGGCTGCTACAATCTCCTTTGTTCGGGTTTCATACAAATCAACAGTCAAATAGCAATGGGAGCAAGTATCTCTCCGGTATCTGGCTTCCATAACCCGCAATACGATATAAGTATTACCATTTGGAAGGATCCAAAAGAAGGGCACTGGTGGATGCAGTTTGGAGACGGGTATGTGTTGGGATACTGGCCAAGCTTTCTATTCTCTTACTTAGCAGATAGTGCATCAATAGTAGAGTGGGGTGGAGAAGTAGTGAACATGGAAGAAGATGGTCACCACACAACCACGCAAATGGGAAGCGGTCAATTTCCGGATGAAGGGTTCACGAAAGCGAGTTACTTCAGGAACATTCAAGTCGTTGATAGCTCTAATAATCTGAAAGAACCAAAAGGGCTCAACACTTTCACTGAGAAATCGAATTGCTATGATGTTGAGGTTGGTAAGAATGATGATTGGGGACATTATTTTTACTATGGAGGTCCTGGAAGAAACCCTAATTGTCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Neprosin Neprosin_AP

Protein Analysis

420

Amino Acids

47.23

Weight (kDa)

6.43

Isoelectric Point (pI)

38.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Neprosin_AP PF14365 56 - 178 2.2e-47 Neprosin activation peptide
Neprosin PF03080 202 - 413 1.5e-85 Neprosin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 673
AccB1I GGYRCC 1 cut(s) 354
AccIII TCCGGA 1 cut(s) 1066
AciI CCGC 3 cut(s) 8, 854, 1056
AclWI GGATC 3 cut(s) 482, 881, 894
AcoI YGGCCR 1 cut(s) 944
AcsI RAATTY 1 cut(s) 115
AcuI CTGAAG 3 cut(s) 19, 396, 1081
AfiI CCNNNNNNNGG 4 cut(s) 781, 839, 896, 1073
AgsI TTSAA 6 cut(s) 108, 122, 139, 148, 671, 1109
AjnI CCWGG 1 cut(s) 1237
AjuI GAANNNNNNNTTGG 2 cut(s) 276, 308
AloI GAACNNNNNNTCC 2 cut(s) 1061, 1093
AluBI AGCT 8 cut(s) 224, 530, 600, 615, 674, 755, 951, 1122
AluI AGCT 8 cut(s) 224, 530, 600, 615, 674, 755, 951, 1122
Alw21I GWGCWC 1 cut(s) 129
Alw26I GTCTC 4 cut(s) 81, 88, 624, 915
Alw44I GTGCAC 1 cut(s) 125
AlwI GGATC 3 cut(s) 482, 881, 894
Aor13HI TCCGGA 1 cut(s) 1066
AoxI GGCC 1 cut(s) 944
ApaLI GTGCAC 1 cut(s) 125
ApeKI GCWGC 2 cut(s) 5, 762
ApoI RAATTY 1 cut(s) 115
ArsI GACNNNNNNTTYG 2 cut(s) 787, 819
AspLEI GCGC 2 cut(s) 13, 89
AspS9I GGNCC 2 cut(s) 266, 1235
AsuHPI GGTGA 4 cut(s) 197, 224, 308, 1022
AvaII GGWCC 2 cut(s) 266, 1235
BaeGI GKGCMC 2 cut(s) 129, 903
BalI TGGCCA 1 cut(s) 946
BamHI GGATCC 1 cut(s) 886
BanI GGYRCC 1 cut(s) 354
BanII GRGCYC 1 cut(s) 1152
BarI GAAGNNNNNNTAC 2 cut(s) 369, 401
BbsI GAAGAC 1 cut(s) 25
Bbv12I GWGCWC 1 cut(s) 129
BbvI GCAGC 1 cut(s) 749
BccI CCATC 3 cut(s) 176, 256, 1019
BceAI ACGGC 1 cut(s) 143
BciT130I CCWGG 1 cut(s) 1239
BciVI GTATCC 1 cut(s) 932
BclI TGATCA 1 cut(s) 229
BcoDI GTCTC 4 cut(s) 81, 88, 624, 915
BfaI CTAG 1 cut(s) 270
BfmI CTRYAG 1 cut(s) 525
BfuAI ACCTGC 1 cut(s) 673
BfuI GTATCC 1 cut(s) 932
BglII AGATCT 1 cut(s) 655
BisI GCNGC 3 cut(s) 6, 9, 763
BlsI GCNGC 3 cut(s) 7, 10, 764
Bme1390I CCNGG 1 cut(s) 1239
Bme18I GGWCC 2 cut(s) 266, 1235
BmgT120I GGNCC 2 cut(s) 266, 1235
BmiI GGNNCC 4 cut(s) 267, 356, 585, 888
BmrFI CCNGG 1 cut(s) 1239
BmsI GCATC 3 cut(s) 733, 900, 989
BoxI GACNNNNGTC 1 cut(s) 73
BpiI GAAGAC 1 cut(s) 25
BpmI CTGGAG 1 cut(s) 264
BpuEI CTTGAG 1 cut(s) 439
Bsa29I ATCGAT 1 cut(s) 142
BsaBI GATNNNNATC 2 cut(s) 492, 747
BsaWI WCCGGW 2 cut(s) 832, 1066
BsaXI ACNNNNNCTCC 4 cut(s) 85, 115, 993, 1023
Bsc4I CCNNNNNNNGG 4 cut(s) 781, 839, 896, 1073
Bse1I ACTGG 4 cut(s) 389, 737, 908, 947
Bse3DI GCAATG 1 cut(s) 819
Bse8I GATNNNNATC 2 cut(s) 492, 747
BseAI TCCGGA 1 cut(s) 1066
BseBI CCWGG 1 cut(s) 1239
BseCI ATCGAT 1 cut(s) 142
BseGI GGATG 3 cut(s) 418, 915, 1075
BseJI GATNNNNATC 2 cut(s) 492, 747
BseLI CCNNNNNNNGG 4 cut(s) 781, 839, 896, 1073
BseMI GCAATG 1 cut(s) 819
BseMII CTCAG 4 cut(s) 112, 303, 635, 1155
BseNI ACTGG 4 cut(s) 389, 737, 908, 947
BseRI GAGGAG 1 cut(s) 409
BseSI GKGCMC 2 cut(s) 129, 903
BseXI GCAGC 1 cut(s) 749
BseYI CCCAGC 1 cut(s) 89
BshFI GGCC 1 cut(s) 946
BshNI GGYRCC 1 cut(s) 354
BshVI ATCGAT 1 cut(s) 142
BsiHKAI GWGCWC 1 cut(s) 129
BsiSI CCGG 2 cut(s) 833, 1067
BslFI GGGAC 4 cut(s) 81, 279, 394, 1227
BslI CCNNNNNNNGG 4 cut(s) 781, 839, 896, 1073
BsmAI GTCTC 4 cut(s) 81, 88, 624, 915
BsmBI CGTCTC 1 cut(s) 915
BsmFI GGGAC 4 cut(s) 81, 279, 394, 1227
BsmI GAATGC 1 cut(s) 168
BsnI GGCC 1 cut(s) 946
Bsp1286I GDGCHC 3 cut(s) 129, 903, 1152
Bsp13I TCCGGA 1 cut(s) 1066
Bsp143I GATC 7 cut(s) 229, 247, 479, 487, 493, 655, 886
BspACI CCGC 3 cut(s) 8, 854, 1056
BspANI GGCC 1 cut(s) 946
BspCNI CTCAG 4 cut(s) 111, 304, 634, 1156
BspDI ATCGAT 1 cut(s) 142
BspEI TCCGGA 1 cut(s) 1066
BspLI GGNNCC 4 cut(s) 267, 356, 585, 888
BspMI ACCTGC 1 cut(s) 673
BspPI GGATC 3 cut(s) 482, 881, 894
BspT107I GGYRCC 1 cut(s) 354
BsrDI GCAATG 1 cut(s) 819
BsrI ACTGG 4 cut(s) 389, 737, 908, 947
BssMI GATC 7 cut(s) 229, 247, 479, 487, 493, 655, 886
Bst2UI CCWGG 1 cut(s) 1239
Bst4CI ACNGT 4 cut(s) 200, 348, 441, 803
BstAPI GCANNNNNTGC 1 cut(s) 977
BstC8I GCNNGC 1 cut(s) 522
BstDEI CTNAG 5 cut(s) 98, 312, 621, 967, 1164
BstEII GGTNACC 1 cut(s) 1028
BstF5I GGATG 3 cut(s) 418, 915, 1075
BstHHI GCGC 2 cut(s) 13, 89
BstKTI GATC 7 cut(s) 232, 250, 482, 490, 496, 658, 889
BstMAI GTCTC 4 cut(s) 81, 88, 624, 915
BstMBI GATC 7 cut(s) 229, 247, 479, 487, 493, 655, 886
BstMWI GCNNNNNNNGC 3 cut(s) 752, 818, 977
BstNI CCWGG 1 cut(s) 1239
BstNSI RCATGY 1 cut(s) 87
BstPAI GACNNNNGTC 1 cut(s) 73
BstPI GGTNACC 1 cut(s) 1028
BstSCI CCNGG 1 cut(s) 1237
BstSFI CTRYAG 1 cut(s) 525
BstSLI GKGCMC 2 cut(s) 129, 903
BstV1I GCAGC 1 cut(s) 749
BstV2I GAAGAC 1 cut(s) 25
BstX2I RGATCY 2 cut(s) 655, 886
BstXI CCANNNNNNTGG 1 cut(s) 1048
BstYI RGATCY 2 cut(s) 655, 886
Bsu15I ATCGAT 1 cut(s) 142
BsuI GTATCC 1 cut(s) 932
BsuRI GGCC 1 cut(s) 946
BsuTUI ATCGAT 1 cut(s) 142
BtgZI GCGATG 1 cut(s) 756
BtsCI GGATG 3 cut(s) 418, 915, 1075
BtsIMutI CAGTG 4 cut(s) 106, 396, 901, 1161
BveI ACCTGC 1 cut(s) 673
Cac8I GCNNGC 1 cut(s) 522
CfoI GCGC 2 cut(s) 13, 89
Cfr13I GGNCC 2 cut(s) 266, 1235
ClaI ATCGAT 1 cut(s) 142
CviAII CATG 2 cut(s) 84, 1015
DdeI CTNAG 5 cut(s) 98, 312, 621, 967, 1164
DpnI GATC 7 cut(s) 231, 249, 481, 489, 495, 657, 888
DpnII GATC 7 cut(s) 229, 247, 479, 487, 493, 655, 886
EaeI YGGCCR 1 cut(s) 944
Eco24I GRGCYC 1 cut(s) 1152
Eco47I GGWCC 2 cut(s) 266, 1235
Eco57I CTGAAG 3 cut(s) 19, 396, 1081
Eco91I GGTNACC 1 cut(s) 1028
EcoO109I RGGNCCY 2 cut(s) 266, 1235
EcoO65I GGTNACC 1 cut(s) 1028
EcoRII CCWGG 1 cut(s) 1237
EcoT38I GRGCYC 1 cut(s) 1152
Esp3I CGTCTC 1 cut(s) 915
FaeI CATG 2 cut(s) 87, 1018
FaqI GGGAC 4 cut(s) 81, 279, 394, 1227
FatI CATG 2 cut(s) 83, 1014
FauI CCCGC 1 cut(s) 861
FbaI TGATCA 1 cut(s) 229
Fnu4HI GCNGC 3 cut(s) 6, 9, 763
FokI GGATG 3 cut(s) 425, 922, 1082
FriOI GRGCYC 1 cut(s) 1152
Fsp4HI GCNGC 3 cut(s) 6, 9, 763
FspBI CTAG 1 cut(s) 270
GlaI GCGC 2 cut(s) 12, 88
GluI GCNGC 3 cut(s) 6, 9, 763
GsaI CCCAGC 1 cut(s) 93
GsuI CTGGAG 1 cut(s) 264
HaeIII GGCC 1 cut(s) 946
HapII CCGG 2 cut(s) 833, 1067
HhaI GCGC 2 cut(s) 13, 89
Hin1II CATG 2 cut(s) 87, 1018
Hin6I GCGC 2 cut(s) 11, 87
HinP1I GCGC 2 cut(s) 11, 87
HindIII AAGCTT 1 cut(s) 949
HinfI GANTC 6 cut(s) 96, 139, 175, 256, 284, 631
HpaII CCGG 2 cut(s) 833, 1067
HphI GGTGA 4 cut(s) 197, 224, 308, 1022
Hpy166II GTNNAC 4 cut(s) 127, 205, 1012, 1080
Hpy188I TCNGA 6 cut(s) 38, 174, 261, 313, 376, 1134
Hpy188III TCNNGA 9 cut(s) 179, 281, 418, 491, 653, 692, 1067, 1081, 1099
Hpy8I GTNNAC 4 cut(s) 127, 205, 1012, 1080
HpyAV CCTTC 5 cut(s) 371, 533, 877, 890, 1067
HpyCH4III ACNGT 4 cut(s) 200, 348, 441, 803
HpyCH4V TGCA 4 cut(s) 127, 595, 913, 980
HpyF10VI GCNNNNNNNGC 3 cut(s) 752, 818, 977
HpyF3I CTNAG 5 cut(s) 98, 312, 621, 967, 1164
Hsp92II CATG 2 cut(s) 87, 1018
HspAI GCGC 2 cut(s) 11, 87
Kpn2I TCCGGA 1 cut(s) 1066
Ksp22I TGATCA 1 cut(s) 229
Kzo9I GATC 7 cut(s) 229, 247, 479, 487, 493, 655, 886
LmnI GCTCC 1 cut(s) 818
Lsp1109I GCAGC 1 cut(s) 749
LweI GCATC 3 cut(s) 733, 900, 989
MaeI CTAG 1 cut(s) 270
MaeIII GTNAC 4 cut(s) 185, 272, 1028, 1091
MalI GATC 7 cut(s) 231, 249, 481, 489, 495, 657, 888
MboI GATC 7 cut(s) 229, 247, 479, 487, 493, 655, 886
MboII GAAGA 5 cut(s) 25, 463, 1031, 1034, 1254
MflI RGATCY 2 cut(s) 655, 886
MhlI GDGCHC 3 cut(s) 129, 903, 1152
MlsI TGGCCA 1 cut(s) 946
MluCI AATT 4 cut(s) 115, 1061, 1174, 1252
MluNI TGGCCA 1 cut(s) 946
MlyI GAGTC 2 cut(s) 90, 184
Mox20I TGGCCA 1 cut(s) 946
MroI TCCGGA 1 cut(s) 1066
MscI TGGCCA 1 cut(s) 946
MseI TTAA 3 cut(s) 162, 435, 501
MslI CAYNNNNRTG 2 cut(s) 349, 1046
Msp20I TGGCCA 1 cut(s) 946
MspI CCGG 2 cut(s) 833, 1067
MspR9I CCNGG 1 cut(s) 1239
Mva1269I GAATGC 1 cut(s) 168
MvaI CCWGG 1 cut(s) 1239
MwoI GCNNNNNNNGC 3 cut(s) 752, 818, 977
NdeII GATC 7 cut(s) 229, 247, 479, 487, 493, 655, 886
NlaIII CATG 2 cut(s) 87, 1018
NlaIV GGNNCC 4 cut(s) 267, 356, 585, 888
NmuCI GTSAC 2 cut(s) 185, 1028
NspI RCATGY 1 cut(s) 87
PctI GAATGC 1 cut(s) 168
PfeI GAWTC 4 cut(s) 139, 256, 284, 631
PfoI TCCNGGA 1 cut(s) 1237
PkrI GCNGC 3 cut(s) 7, 10, 764
PleI GAGTC 2 cut(s) 90, 183
PpsI GAGTC 2 cut(s) 90, 183
PpuMI RGGWCCY 2 cut(s) 266, 1235
PshAI GACNNNNGTC 1 cut(s) 73
Psp5II RGGWCCY 2 cut(s) 266, 1235
Psp6I CCWGG 1 cut(s) 1237
PspEI GGTNACC 1 cut(s) 1028
PspFI CCCAGC 1 cut(s) 89
PspGI CCWGG 1 cut(s) 1237
PspN4I GGNNCC 4 cut(s) 267, 356, 585, 888
PspPI GGNCC 2 cut(s) 266, 1235
PspPPI RGGWCCY 2 cut(s) 266, 1235
PsuI RGATCY 2 cut(s) 655, 886
RseI CAYNNNNRTG 2 cut(s) 349, 1046
SaqAI TTAA 3 cut(s) 162, 435, 501
SatI GCNGC 3 cut(s) 6, 9, 763
Sau3AI GATC 7 cut(s) 229, 247, 479, 487, 493, 655, 886
Sau96I GGNCC 2 cut(s) 266, 1235
SchI GAGTC 2 cut(s) 90, 184
ScrFI CCNGG 1 cut(s) 1239
SduI GDGCHC 3 cut(s) 129, 903, 1152
SfaNI GCATC 3 cut(s) 733, 900, 989
SfcI CTRYAG 1 cut(s) 525
SinI GGWCC 2 cut(s) 266, 1235
SmiMI CAYNNNNRTG 2 cut(s) 349, 1046
SmlI CTYRAG 1 cut(s) 418
SmoI CTYRAG 1 cut(s) 418
Sse9I AATT 4 cut(s) 115, 1061, 1174, 1252
SsiI CCGC 3 cut(s) 8, 854, 1056
SspMI CTAG 1 cut(s) 270
StyD4I CCNGG 1 cut(s) 1237
TaaI ACNGT 4 cut(s) 200, 348, 441, 803
TaqI TCGA 4 cut(s) 142, 212, 478, 1172
TasI AATT 4 cut(s) 115, 1061, 1174, 1252
TauI GCSGC 1 cut(s) 11
TfiI GAWTC 4 cut(s) 139, 256, 284, 631
Tru1I TTAA 3 cut(s) 162, 435, 501
Tru9I TTAA 3 cut(s) 162, 435, 501
TscAI CASTG 4 cut(s) 106, 396, 908, 1168
TseFI GTSAC 2 cut(s) 185, 1028
TseI GCWGC 2 cut(s) 5, 762
Tsp45I GTSAC 2 cut(s) 185, 1028
TspDTI ATGAA 2 cut(s) 778, 1086
TspGWI ACGGA 1 cut(s) 378
TspRI CASTG 4 cut(s) 106, 396, 908, 1168
VneI GTGCAC 1 cut(s) 125
VpaK11BI GGWCC 2 cut(s) 266, 1235
XapI RAATTY 1 cut(s) 115
XceI RCATGY 1 cut(s) 87
XspI CTAG 1 cut(s) 270
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.