Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0004991

Physical Location & Seq

Genomic Coordinates Forward (+)

252675 .. 256341

Gene Structure 3667 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1003500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1254 bp

ATGGAGTTTGCTGGGTGCAGCAGAGGGAGCTCAGGGAGAGCTCTGGCTCTGGCCCTTTGCCTTTTGGCCTTGATATCTCTGTCTTGTGCTGCCAGATTGAGTGCTTCTTCAAGGCAGAACCTTGATGTCCAGAAGCACCTCAACCGCTTGAACAAACCCGCCGTGAAAAGCATCAAGAGTCCAGATGGAGATATTATAGACTGTGTTCGTATATCTCAACAACCGGCTTTCGATCACCCTTACCTCAAAGACCACAAAATCCAGATGAGGCCTAGCTACCACCCAGAAGGGTTGTTTGCTGAGAACAAAGTGGCTGAAAAAACAAAAGAAAAAGCAAATACCCAGCTGTGGCATGTGAATGGGAGATGCCCGGAAGATACTATTCCGGTAAGGAGAACGAAGGAAGATGATATTCTGAGAGCGAGTTCAGTGAAACATTATGGAAGGAAGAAGCAGAGAACCATACCAAAATCTGCAGATCCTGATCTTGCCAATGAAAGTGGTCATCAGCATGCAATTGCTTATGTCAATGGAGATAAGTATTTTGGAGCTAAGGCAACCATAAATGTCTGGGAGCCCAAGATACAACAGCCTAATGAGTTCAGCTTGTCTCAGCTGTGGGTATTAGGAGGGTCTTTTGGTGAAGATCTGAACAGCATTGAAGCTGGCTGGCAGGTCAGCCCGGACCTCTATGGTGACAACAACACAAGACTGTTTACCTACTGGACTAGTGATGCATATCAAGCCACCGGTTGCTACAACCTCCTCTGCTCAGGCTTCATTCAAATCAACAGCGAAATAGCAATGGGTGCAAGCATCTCTCCCGTTTCGGGTTTACGAGGATCGCAGTATGATATCAGCATACTCGTCTGGAAGGATCCAAAGGAGGGGCATTGGTGGATGCAATTCGGGAACGACTATGTGCTGGGTTATTGGCCTTCGTTCCTCTTCTCATACTTGGCTGACAGTGCTTCCATGATTGAGTGGGGAGGTGAGGTTGTGAATTCACAACCGGACGGCCAGCACACATCGACCCAAATGGGCAGCGGACGTTTTCCGGAGGAAGGTTTTGGCAAGGCAAGTTACTTCAGAAACATTCAAGTTGTTGATAGCTCAAATAATCTCAAGGTCCCCAAAGGGTTGGGCACCTTCACCGAGCAATCAAACTGCTATGATGTTCAAACTGGAAGTAATGGGGATTGGGGTCATTACTTTTACTACGGAGGACCCGGTAAAAACCCGAATTGCCAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Neprosin Neprosin_AP

Protein Analysis

418

Amino Acids

46.37

Weight (kDa)

6.6

Isoelectric Point (pI)

39.0

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Neprosin_AP	PF14365	57 - 175	6.4e-48	Neprosin activation peptide
Neprosin	PF03080	199 - 410	2.1e-86	Neprosin

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0004991)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G55360 AT3G13510 AT5G56530 AT5G56530
fragaria_vesca	FvH4_3g45750 FvH4_3g45750 FvH4_3g45750 FvH4_3g45750 FvH4_3g45750 FvH4_3g45750
malus_domestica	MD03G1003500.v1.1 MD11G1003400.v1.1
prunus_persica	Prupe.6G003200_v2.0.a1 Prupe.6G003200_v2.0.a1 Prupe.6G003200_v2.0.a1
pyrus_communis	pycom03g00350 pycom11g00300
rosa_chinensis	RchiOBHm_Chr5g0083821
rosa_laevigata	RLG00000037054
rosa_multiflora	Rmu_sc0024007.1_g000001
rosa_roxburghii	Rroxscaffold_1G00000770
rosa_rugosa	Rorug05G0486300 Rorug05G0486300 Rorug05G0486300 Rorug05G0486300 Rorug05G0486300
rosa_samantha	Rh5AG539300 Rh5CG586500 Rh5DG573000
rosa_wichuraiana	Rw5G050140

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	664
AccB1I	GGYRCC	1 cut(s)	1145
AccIII	TCCGGA	1 cut(s)	1057
AciI	CCGC	3 cut(s)	145, 159, 1047
AclWI	GGATC	4 cut(s)	473, 850, 872, 885
AcoI	YGGCCR	1 cut(s)	1018
AcsI	RAATTY	1 cut(s)	1003
AcuI	CTGAAG	1 cut(s)	1072
AfiI	CCNNNNNNNGG	4 cut(s)	348, 830, 887, 1064
AgeI	ACCGGT	1 cut(s)	749
AgsI	TTSAA	6 cut(s)	111, 151, 662, 785, 1100, 1181
AhlI	ACTAGT	1 cut(s)	728
AluBI	AGCT	9 cut(s)	30, 41, 276, 346, 551, 606, 616, 665, 1113
AluI	AGCT	9 cut(s)	30, 41, 276, 346, 551, 606, 616, 665, 1113
Alw21I	GWGCWC	2 cut(s)	32, 43
Alw26I	GTCTC	1 cut(s)	615
AlwI	GGATC	4 cut(s)	473, 850, 872, 885
AlwNI	CAGNNNCTG	1 cut(s)	482
Aor13HI	TCCGGA	1 cut(s)	1057
AoxI	GGCC	5 cut(s)	51, 66, 269, 935, 1018
ApeKI	GCWGC	3 cut(s)	18, 89, 1044
ApoI	RAATTY	1 cut(s)	1003
AsiGI	ACCGGT	1 cut(s)	749
AspS9I	GGNCC	4 cut(s)	52, 685, 1129, 1226
AsuC2I	CCSGG	3 cut(s)	371, 683, 1230
AsuHPI	GGTGA	5 cut(s)	227, 653, 707, 1004, 1144
AvaII	GGWCC	3 cut(s)	685, 1129, 1226
BaeGI	GKGCMC	1 cut(s)	1148
BamHI	GGATCC	1 cut(s)	877
BanI	GGYRCC	1 cut(s)	1145
BanII	GRGCYC	3 cut(s)	32, 43, 579
Bbv12I	GWGCWC	2 cut(s)	32, 43
BbvI	GCAGC	3 cut(s)	30, 76, 1056
BccI	CCATC	1 cut(s)	179
BceAI	ACGGC	2 cut(s)	146, 1033
BcnI	CCSGG	3 cut(s)	371, 683, 1230
BcoDI	GTCTC	1 cut(s)	615
BcuI	ACTAGT	1 cut(s)	728
BfaI	CTAG	2 cut(s)	273, 729
BfmI	CTRYAG	1 cut(s)	474
BfuAI	ACCTGC	1 cut(s)	664
BglII	AGATCT	1 cut(s)	646
BisI	GCNGC	3 cut(s)	19, 90, 1045
BlsI	GCNGC	3 cut(s)	20, 91, 1046
Bme1390I	CCNGG	3 cut(s)	371, 683, 1230
Bme18I	GGWCC	3 cut(s)	685, 1129, 1226
BmgT120I	GGNCC	4 cut(s)	52, 685, 1129, 1226
BmiI	GGNNCC	5 cut(s)	576, 879, 1131, 1147, 1228
BmrFI	CCNGG	3 cut(s)	371, 683, 1230
BmsI	GCATC	5 cut(s)	180, 356, 724, 825, 891
Bpu10I	CCTNAGC	3 cut(s)	31, 552, 772
BpuEI	CTTGAG	1 cut(s)	1109
BpuMI	CCSGG	3 cut(s)	371, 683, 1230
BsaBI	GATNNNNATC	2 cut(s)	483, 738
BsaWI	WCCGGW	4 cut(s)	385, 749, 1012, 1057
BsaXI	ACNNNNNCTCC	2 cut(s)	981, 1011
Bsc4I	CCNNNNNNNGG	4 cut(s)	348, 830, 887, 1064
Bse118I	RCCGGY	2 cut(s)	223, 749
Bse1I	ACTGG	3 cut(s)	728, 1189, 1249
Bse3DI	GCAATG	1 cut(s)	810
Bse8I	GATNNNNATC	2 cut(s)	483, 738
BseAI	TCCGGA	1 cut(s)	1057
BseGI	GGATG	1 cut(s)	906
BseJI	GATNNNNATC	2 cut(s)	483, 738
BseLI	CCNNNNNNNGG	4 cut(s)	348, 830, 887, 1064
BseMI	GCAATG	1 cut(s)	810
BseMII	CTCAG	5 cut(s)	45, 291, 407, 626, 786
BseNI	ACTGG	3 cut(s)	728, 1189, 1249
BseRI	GAGGAG	1 cut(s)	755
BseSI	GKGCMC	1 cut(s)	1148
BseXI	GCAGC	3 cut(s)	30, 76, 1056
BseYI	CCCAGC	3 cut(s)	11, 342, 925
BsgI	GTGCAG	1 cut(s)	37
BshFI	GGCC	5 cut(s)	53, 68, 271, 937, 1020
BshNI	GGYRCC	1 cut(s)	1145
BshTI	ACCGGT	1 cut(s)	749
BsiHKAI	GWGCWC	2 cut(s)	32, 43
BsiSI	CCGG	8 cut(s)	224, 371, 386, 683, 750, 1013, 1058, 1230
BslFI	GGGAC	1 cut(s)	1115
BslI	CCNNNNNNNGG	4 cut(s)	348, 830, 887, 1064
BsmAI	GTCTC	1 cut(s)	615
BsmFI	GGGAC	1 cut(s)	1115
BsnI	GGCC	5 cut(s)	53, 68, 271, 937, 1020
Bsp1286I	GDGCHC	4 cut(s)	32, 43, 579, 1148
Bsp13I	TCCGGA	1 cut(s)	1057
Bsp143I	GATC	6 cut(s)	232, 478, 484, 646, 842, 877
BspACI	CCGC	3 cut(s)	145, 159, 1047
BspANI	GGCC	5 cut(s)	53, 68, 271, 937, 1020
BspCNI	CTCAG	5 cut(s)	44, 292, 408, 625, 785
BspEI	TCCGGA	1 cut(s)	1057
BspLI	GGNNCC	5 cut(s)	576, 879, 1131, 1147, 1228
BspMAI	CTGCAG	1 cut(s)	478
BspMI	ACCTGC	1 cut(s)	664
BspPI	GGATC	4 cut(s)	473, 850, 872, 885
BspT107I	GGYRCC	1 cut(s)	1145
BsrDI	GCAATG	1 cut(s)	810
BsrFI	RCCGGY	2 cut(s)	223, 749
BsrI	ACTGG	3 cut(s)	728, 1189, 1249
BssAI	RCCGGY	2 cut(s)	223, 749
BssMI	GATC	6 cut(s)	232, 478, 484, 646, 842, 877
Bst4CI	ACNGT	3 cut(s)	203, 714, 968
Bst6I	CTCTTC	1 cut(s)	953
BstAPI	GCANNNNNTGC	1 cut(s)	809
BstC8I	GCNNGC	5 cut(s)	513, 667, 671, 814, 1022
BstDEI	CTNAG	6 cut(s)	31, 300, 416, 552, 612, 772
BstF5I	GGATG	1 cut(s)	906
BstKTI	GATC	6 cut(s)	235, 481, 487, 649, 845, 880
BstMAI	GTCTC	1 cut(s)	615
BstMBI	GATC	6 cut(s)	232, 478, 484, 646, 842, 877
BstMWI	GCNNNNNNNGC	4 cut(s)	27, 743, 809, 968
BstNSI	RCATGY	2 cut(s)	356, 515
BstSCI	CCNGG	3 cut(s)	369, 681, 1228
BstSFI	CTRYAG	1 cut(s)	474
BstSLI	GKGCMC	1 cut(s)	1148
BstV1I	GCAGC	3 cut(s)	30, 76, 1056
BstX2I	RGATCY	3 cut(s)	478, 646, 877
BstXI	CCANNNNNNTGG	1 cut(s)	1141
BstYI	RGATCY	3 cut(s)	478, 646, 877
BsuRI	GGCC	5 cut(s)	53, 68, 271, 937, 1020
BtsCI	GGATG	1 cut(s)	906
BtsIMutI	CAGTG	2 cut(s)	435, 973
BveI	ACCTGC	1 cut(s)	664
Cac8I	GCNNGC	5 cut(s)	513, 667, 671, 814, 1022
CaiI	CAGNNNCTG	1 cut(s)	482
Cfr10I	RCCGGY	2 cut(s)	223, 749
Cfr13I	GGNCC	4 cut(s)	52, 685, 1129, 1226
CspAI	ACCGGT	1 cut(s)	749
CspCI	CAANNNNNGTGG	2 cut(s)	481, 516
CviAII	CATG	3 cut(s)	353, 512, 976
DdeI	CTNAG	6 cut(s)	31, 300, 416, 552, 612, 772
DpnI	GATC	6 cut(s)	234, 480, 486, 648, 844, 879
DpnII	GATC	6 cut(s)	232, 478, 484, 646, 842, 877
EaeI	YGGCCR	1 cut(s)	1018
Eam1104I	CTCTTC	1 cut(s)	953
EarI	CTCTTC	1 cut(s)	953
Ecl136II	GAGCTC	2 cut(s)	30, 41
Eco147I	AGGCCT	1 cut(s)	271
Eco24I	GRGCYC	3 cut(s)	32, 43, 579
Eco32I	GATATC	2 cut(s)	75, 856
Eco47I	GGWCC	3 cut(s)	685, 1129, 1226
Eco53kI	GAGCTC	2 cut(s)	30, 41
Eco57I	CTGAAG	1 cut(s)	1072
EcoICRI	GAGCTC	2 cut(s)	30, 41
EcoO109I	RGGNCCY	2 cut(s)	1129, 1226
EcoRI	GAATTC	1 cut(s)	1003
EcoRV	GATATC	2 cut(s)	75, 856
EcoT22I	ATGCAT	1 cut(s)	739
EcoT38I	GRGCYC	3 cut(s)	32, 43, 579
FaeI	CATG	3 cut(s)	356, 515, 979
FaqI	GGGAC	1 cut(s)	1115
FatI	CATG	3 cut(s)	352, 511, 975
FauI	CCCGC	1 cut(s)	166
Fnu4HI	GCNGC	3 cut(s)	19, 90, 1045
FokI	GGATG	1 cut(s)	913
FriOI	GRGCYC	3 cut(s)	32, 43, 579
Fsp4HI	GCNGC	3 cut(s)	19, 90, 1045
FspBI	CTAG	2 cut(s)	273, 729
GluI	GCNGC	3 cut(s)	19, 90, 1045
GsaI	CCCAGC	3 cut(s)	15, 346, 929
HaeIII	GGCC	5 cut(s)	53, 68, 271, 937, 1020
HapII	CCGG	8 cut(s)	224, 371, 386, 683, 750, 1013, 1058, 1230
Hin1II	CATG	3 cut(s)	356, 515, 979
HinfI	GANTC	1 cut(s)	178
HpaII	CCGG	8 cut(s)	224, 371, 386, 683, 750, 1013, 1058, 1230
HphI	GGTGA	5 cut(s)	227, 653, 707, 1004, 1144
Hpy166II	GTNNAC	2 cut(s)	717, 836
Hpy188I	TCNGA	3 cut(s)	417, 651, 1091
Hpy188III	TCNNGA	8 cut(s)	130, 175, 182, 262, 482, 871, 910, 1058
Hpy8I	GTNNAC	2 cut(s)	717, 836
HpyAV	CCTTC	7 cut(s)	281, 394, 438, 868, 948, 1058, 1159
HpyCH4III	ACNGT	3 cut(s)	203, 714, 968
HpyCH4IV	ACGT	1 cut(s)	1051
HpyCH4V	TGCA	6 cut(s)	18, 476, 515, 737, 812, 904
HpyF10VI	GCNNNNNNNGC	4 cut(s)	27, 743, 809, 968
HpyF3I	CTNAG	6 cut(s)	31, 300, 416, 552, 612, 772
HpySE526I	ACGT	1 cut(s)	1051
Hsp92II	CATG	3 cut(s)	356, 515, 979
Kpn2I	TCCGGA	1 cut(s)	1057
Kzo9I	GATC	6 cut(s)	232, 478, 484, 646, 842, 877
LmnI	GCTCC	3 cut(s)	27, 548, 574
Lsp1109I	GCAGC	3 cut(s)	30, 76, 1056
LweI	GCATC	5 cut(s)	180, 356, 724, 825, 891
MaeI	CTAG	2 cut(s)	273, 729
MaeII	ACGT	1 cut(s)	1051
MaeIII	GTNAC	2 cut(s)	695, 1082
MalI	GATC	6 cut(s)	234, 480, 486, 648, 844, 879
MboI	GATC	6 cut(s)	232, 478, 484, 646, 842, 877
MboII	GAAGA	6 cut(s)	99, 386, 416, 460, 656, 940
MfeI	CAATTG	1 cut(s)	516
MflI	RGATCY	3 cut(s)	478, 646, 877
MhlI	GDGCHC	4 cut(s)	32, 43, 579, 1148
MluCI	AATT	4 cut(s)	516, 905, 1003, 1243
MlyI	GAGTC	1 cut(s)	187
Mph1103I	ATGCAT	1 cut(s)	739
MroI	TCCGGA	1 cut(s)	1057
MslI	CAYNNNNRTG	2 cut(s)	357, 510
MspA1I	CMGCKG	3 cut(s)	346, 616, 1047
MspI	CCGG	8 cut(s)	224, 371, 386, 683, 750, 1013, 1058, 1230
MspR9I	CCNGG	3 cut(s)	371, 683, 1230
MunI	CAATTG	1 cut(s)	516
MwoI	GCNNNNNNNGC	4 cut(s)	27, 743, 809, 968
NciI	CCSGG	3 cut(s)	371, 683, 1230
NdeII	GATC	6 cut(s)	232, 478, 484, 646, 842, 877
NlaIII	CATG	3 cut(s)	356, 515, 979
NlaIV	GGNNCC	5 cut(s)	576, 879, 1131, 1147, 1228
NmuCI	GTSAC	1 cut(s)	695
NsiI	ATGCAT	1 cut(s)	739
NspI	RCATGY	2 cut(s)	356, 515
PaeI	GCATGC	1 cut(s)	515
PceI	AGGCCT	1 cut(s)	271
PinAI	ACCGGT	1 cut(s)	749
PkrI	GCNGC	3 cut(s)	20, 91, 1046
PleI	GAGTC	1 cut(s)	186
PpsI	GAGTC	1 cut(s)	186
PpuMI	RGGWCCY	2 cut(s)	1129, 1226
Psp124BI	GAGCTC	2 cut(s)	32, 43
Psp5II	RGGWCCY	2 cut(s)	1129, 1226
PspFI	CCCAGC	3 cut(s)	11, 342, 925
PspN4I	GGNNCC	5 cut(s)	576, 879, 1131, 1147, 1228
PspPI	GGNCC	4 cut(s)	52, 685, 1129, 1226
PspPPI	RGGWCCY	2 cut(s)	1129, 1226
PstI	CTGCAG	1 cut(s)	478
PstNI	CAGNNNCTG	1 cut(s)	482
PsuI	RGATCY	3 cut(s)	478, 646, 877
PvuII	CAGCTG	2 cut(s)	346, 616
RseI	CAYNNNNRTG	2 cut(s)	357, 510
SacI	GAGCTC	2 cut(s)	32, 43
SatI	GCNGC	3 cut(s)	19, 90, 1045
Sau3AI	GATC	6 cut(s)	232, 478, 484, 646, 842, 877
Sau96I	GGNCC	4 cut(s)	52, 685, 1129, 1226
SchI	GAGTC	1 cut(s)	187
ScrFI	CCNGG	3 cut(s)	371, 683, 1230
SduI	GDGCHC	4 cut(s)	32, 43, 579, 1148
SfaNI	GCATC	5 cut(s)	180, 356, 724, 825, 891
SfcI	CTRYAG	1 cut(s)	474
SinI	GGWCC	3 cut(s)	685, 1129, 1226
SmiMI	CAYNNNNRTG	2 cut(s)	357, 510
SmlI	CTYRAG	1 cut(s)	1124
SmoI	CTYRAG	1 cut(s)	1124
SpeI	ACTAGT	1 cut(s)	728
SphI	GCATGC	1 cut(s)	515
Sse9I	AATT	4 cut(s)	516, 905, 1003, 1243
SseBI	AGGCCT	1 cut(s)	271
SsiI	CCGC	3 cut(s)	145, 159, 1047
SspMI	CTAG	2 cut(s)	273, 729
SstI	GAGCTC	2 cut(s)	32, 43
StuI	AGGCCT	1 cut(s)	271
StyD4I	CCNGG	3 cut(s)	369, 681, 1228
TaaI	ACNGT	3 cut(s)	203, 714, 968
TaiI	ACGT	1 cut(s)	1054
TaqI	TCGA	2 cut(s)	231, 1031
TasI	AATT	4 cut(s)	516, 905, 1003, 1243
TscAI	CASTG	2 cut(s)	435, 973
TseFI	GTSAC	1 cut(s)	695
TseI	GCWGC	3 cut(s)	18, 89, 1044
Tsp45I	GTSAC	1 cut(s)	695
TspDTI	ATGAA	2 cut(s)	510, 769
TspGWI	ACGGA	1 cut(s)	1236
TspRI	CASTG	2 cut(s)	435, 973
VpaK11BI	GGWCC	3 cut(s)	685, 1129, 1226
XapI	RAATTY	1 cut(s)	1003
XceI	RCATGY	2 cut(s)	356, 515
XspI	CTAG	2 cut(s)	273, 729
Zsp2I	ATGCAT	1 cut(s)	739

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.