Rh5CG586500

Neprosin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0004991
Physical Location & Seq
Reverse (-)
83173772 .. 83178478
4707 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG586500.1

Sequence Viewer

Length: 990 bp
ATGAGGCCTAACTACCACCCAGAAGGCTTATTTCAAGAAAACAAAGTTGCTGAAAAATCGAAAGAAATGCCAAACACCCAGCTGTGGCATGTGAATGGTAGATGCCCTGAAGATACCATACCCGTGAGGAGGACGAAGAAAGATGATATTCTGAGAGCAAGCTCAGTCAAAAGATATGGGCGGAAGAAGGAGAAAACCATTCCAAAGTCTGCAGATCCTGATCTTGCCAATGAGAGTGGTCATCAGCATGCAATTGCTTATGTAAATGGAGACAAGTATTTTGGAGCAAAAGCAACCATCAATGTCTGGGAGCCCAAGATTCAACAGCCTAATGAGTTCAGCTTGTCTCAGCTTTGGATATTAGGAGGGTCTTTTGGTGAAGACCTGAACAGCATTGAAGCTGGTTGGCAGGTCAGCCCAGACCTCTATGGTGACAACAACACAAGACTATTTACCTATTGGACTAGTGATGCATATCAAGCCACTGGTTGCTACAACCTCCTGTGCTCGGGCTTTATTCAAATCAACAGTGAAATAGCAATGGGCGCAAGCATCTCTCCTGTGTCAAGCTACCGAACTTCCCAATACGATATCAGTATACTTGTCTGGAAGGATCCAAAGGAGGGACATTGGTGGATGCAGTTCGGCAATGACTATGTGTTGGGGTATTGGCCTTCTTTCCTGTTCTCGTACTTGGCCGACAGTGCTTCCATGATTGAGTGGGGAGGTGAGGTTGTAAATTCGCAGTCGGATGGCCGACACACCTCAACTCAAATGGGCAGTGGTCGTTTTCCGGAGGAGGGTTTTGGAAAATCAAGTTATTTCAGGAACATTCAAGTTGTTGATAGCTCCAATAATCTCAAGGCCCCCAAAGGGTTAGGCACCTTCACCGAGCAGTCAAACTGCTATGATGTTCAGACAGGAAGTAATGGGGATTGGGGTCACTACTTTTACTATGGAGGACCAGGTAGAAACGCAAATTGCCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Neprosin Neprosin_AP

Protein Analysis

329

Amino Acids

37.03

Weight (kDa)

5.84

Isoelectric Point (pI)

42.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Neprosin_AP PF14365 1 - 87 6e-25 Neprosin activation peptide
Neprosin PF03080 111 - 322 4.8e-87 Neprosin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 400
AccB1I GGYRCC 1 cut(s) 881
AccI GTMKAC 1 cut(s) 598
AccIII TCCGGA 1 cut(s) 793
AciI CCGC 1 cut(s) 181
AclWI GGATC 3 cut(s) 209, 608, 621
AcoI YGGCCR 2 cut(s) 696, 754
AcsI RAATTY 1 cut(s) 739
AcuI CTGAAG 1 cut(s) 129
AfaI GTAC 1 cut(s) 692
AfiI CCNNNNNNNGG 6 cut(s) 84, 129, 508, 623, 800, 873
AgsI TTSAA 5 cut(s) 35, 323, 398, 521, 836
AhlI ACTAGT 1 cut(s) 464
AjnI CCWGG 1 cut(s) 964
AluBI AGCT 7 cut(s) 82, 162, 342, 352, 401, 570, 849
AluI AGCT 7 cut(s) 82, 162, 342, 352, 401, 570, 849
Alw21I GWGCWC 1 cut(s) 509
Alw26I GTCTC 2 cut(s) 264, 351
AlwI GGATC 3 cut(s) 209, 608, 621
AlwNI CAGNNNCTG 1 cut(s) 218
Ama87I CYCGRG 1 cut(s) 508
Aor13HI TCCGGA 1 cut(s) 793
AoxI GGCC 5 cut(s) 5, 671, 696, 754, 864
ApoI RAATTY 1 cut(s) 739
ArsI GACNNNNNNTTYG 2 cut(s) 263, 295
AspLEI GCGC 1 cut(s) 548
AspS9I GGNCC 2 cut(s) 865, 962
AsuHPI GGTGA 4 cut(s) 389, 443, 740, 880
AvaI CYCGRG 1 cut(s) 508
AvaII GGWCC 1 cut(s) 962
BamHI GGATCC 1 cut(s) 613
BanI GGYRCC 1 cut(s) 881
BanII GRGCYC 1 cut(s) 315
BarI GAAGNNNNNNTAC 2 cut(s) 102, 134
BbsI GAAGAC 1 cut(s) 387
Bbv12I GWGCWC 1 cut(s) 509
BccI CCATC 2 cut(s) 305, 746
BcgI CGANNNNNNTGC 2 cut(s) 49, 83
BciT130I CCWGG 1 cut(s) 966
BcoDI GTCTC 2 cut(s) 264, 351
BcuI ACTAGT 1 cut(s) 464
BfaI CTAG 1 cut(s) 465
BfmI CTRYAG 1 cut(s) 210
BfuAI ACCTGC 1 cut(s) 400
Bme1390I CCNGG 1 cut(s) 966
Bme18I GGWCC 1 cut(s) 962
BmeT110I CYCGRG 1 cut(s) 508
BmgT120I GGNCC 2 cut(s) 865, 962
BmiI GGNNCC 4 cut(s) 312, 615, 867, 883
BmrFI CCNGG 1 cut(s) 966
BmsI GCATC 4 cut(s) 92, 460, 561, 627
BpiI GAAGAC 1 cut(s) 387
BpuEI CTTGAG 1 cut(s) 845
BsaBI GATNNNNATC 2 cut(s) 219, 474
BsaWI WCCGGW 1 cut(s) 793
BsaXI ACNNNNNCTCC 2 cut(s) 717, 747
Bsc4I CCNNNNNNNGG 6 cut(s) 84, 129, 508, 623, 800, 873
Bse1I ACTGG 1 cut(s) 490
Bse3DI GCAATG 2 cut(s) 546, 655
Bse8I GATNNNNATC 2 cut(s) 219, 474
BseAI TCCGGA 1 cut(s) 793
BseBI CCWGG 1 cut(s) 966
BseGI GGATG 2 cut(s) 642, 757
BseJI GATNNNNATC 2 cut(s) 219, 474
BseLI CCNNNNNNNGG 6 cut(s) 84, 129, 508, 623, 800, 873
BseMI GCAATG 2 cut(s) 546, 655
BseMII CTCAG 3 cut(s) 143, 177, 362
BseNI ACTGG 1 cut(s) 490
BseRI GAGGAG 2 cut(s) 142, 812
BseYI CCCAGC 1 cut(s) 78
BshFI GGCC 5 cut(s) 7, 673, 698, 756, 866
BshNI GGYRCC 1 cut(s) 881
BsiHKAI GWGCWC 1 cut(s) 509
BsiHKCI CYCGRG 1 cut(s) 508
BsiSI CCGG 1 cut(s) 794
BslFI GGGAC 1 cut(s) 639
BslI CCNNNNNNNGG 6 cut(s) 84, 129, 508, 623, 800, 873
BsmAI GTCTC 2 cut(s) 264, 351
BsmFI GGGAC 1 cut(s) 639
BsnI GGCC 5 cut(s) 7, 673, 698, 756, 866
BsoBI CYCGRG 1 cut(s) 508
Bsp1286I GDGCHC 2 cut(s) 315, 509
Bsp13I TCCGGA 1 cut(s) 793
Bsp143I GATC 3 cut(s) 214, 220, 613
BspACI CCGC 1 cut(s) 181
BspANI GGCC 5 cut(s) 7, 673, 698, 756, 866
BspCNI CTCAG 3 cut(s) 144, 176, 361
BspEI TCCGGA 1 cut(s) 793
BspLI GGNNCC 4 cut(s) 312, 615, 867, 883
BspMAI CTGCAG 1 cut(s) 214
BspMI ACCTGC 1 cut(s) 400
BspPI GGATC 3 cut(s) 209, 608, 621
BspT107I GGYRCC 1 cut(s) 881
BsrDI GCAATG 2 cut(s) 546, 655
BsrI ACTGG 1 cut(s) 490
BssMI GATC 3 cut(s) 214, 220, 613
BssNAI GTATAC 1 cut(s) 599
Bst1107I GTATAC 1 cut(s) 599
Bst2UI CCWGG 1 cut(s) 966
Bst4CI ACNGT 2 cut(s) 530, 704
BstC8I GCNNGC 3 cut(s) 160, 249, 550
BstDEI CTNAG 3 cut(s) 152, 163, 348
BstF5I GGATG 2 cut(s) 642, 757
BstHHI GCGC 1 cut(s) 548
BstKTI GATC 3 cut(s) 217, 223, 616
BstMAI GTCTC 2 cut(s) 264, 351
BstMBI GATC 3 cut(s) 214, 220, 613
BstMWI GCNNNNNNNGC 3 cut(s) 479, 545, 704
BstNI CCWGG 1 cut(s) 966
BstNSI RCATGY 2 cut(s) 92, 251
BstSCI CCNGG 1 cut(s) 964
BstSFI CTRYAG 1 cut(s) 210
BstV2I GAAGAC 1 cut(s) 387
BstX2I RGATCY 2 cut(s) 214, 613
BstYI RGATCY 2 cut(s) 214, 613
BstZ17I GTATAC 1 cut(s) 599
BsuRI GGCC 5 cut(s) 7, 673, 698, 756, 866
BtsCI GGATG 2 cut(s) 642, 757
BtsI GCAGTG 1 cut(s) 787
BtsIMutI CAGTG 4 cut(s) 483, 535, 709, 787
BveI ACCTGC 1 cut(s) 400
Cac8I GCNNGC 3 cut(s) 160, 249, 550
CaiI CAGNNNCTG 1 cut(s) 218
CfoI GCGC 1 cut(s) 548
Cfr13I GGNCC 2 cut(s) 865, 962
CsiI ACCWGGT 1 cut(s) 964
Csp6I GTAC 1 cut(s) 691
CspCI CAANNNNNGTGG 2 cut(s) 217, 252
CviAII CATG 4 cut(s) 89, 248, 712, 987
CviQI GTAC 1 cut(s) 691
DdeI CTNAG 3 cut(s) 152, 163, 348
DpnI GATC 3 cut(s) 216, 222, 615
DpnII GATC 3 cut(s) 214, 220, 613
EaeI YGGCCR 2 cut(s) 696, 754
EciI GGCGGA 1 cut(s) 196
Eco147I AGGCCT 1 cut(s) 7
Eco24I GRGCYC 1 cut(s) 315
Eco32I GATATC 1 cut(s) 592
Eco47I GGWCC 1 cut(s) 962
Eco57I CTGAAG 1 cut(s) 129
Eco88I CYCGRG 1 cut(s) 508
EcoO109I RGGNCCY 1 cut(s) 865
EcoRII CCWGG 1 cut(s) 964
EcoRV GATATC 1 cut(s) 592
EcoT22I ATGCAT 1 cut(s) 475
EcoT38I GRGCYC 1 cut(s) 315
FaeI CATG 4 cut(s) 92, 251, 715, 990
FaqI GGGAC 1 cut(s) 639
FatI CATG 4 cut(s) 88, 247, 711, 986
FblI GTMKAC 1 cut(s) 598
FokI GGATG 2 cut(s) 649, 764
FriOI GRGCYC 1 cut(s) 315
FspBI CTAG 1 cut(s) 465
GlaI GCGC 1 cut(s) 547
GsaI CCCAGC 1 cut(s) 82
HaeIII GGCC 5 cut(s) 7, 673, 698, 756, 866
HapII CCGG 1 cut(s) 794
HhaI GCGC 1 cut(s) 548
Hin1II CATG 4 cut(s) 92, 251, 715, 990
Hin6I GCGC 1 cut(s) 546
HinP1I GCGC 1 cut(s) 546
HinfI GANTC 1 cut(s) 319
HpaII CCGG 1 cut(s) 794
HphI GGTGA 4 cut(s) 389, 443, 740, 880
Hpy166II GTNNAC 1 cut(s) 599
Hpy188I TCNGA 3 cut(s) 153, 751, 918
Hpy188III TCNNGA 5 cut(s) 35, 218, 607, 794, 826
Hpy8I GTNNAC 1 cut(s) 599
HpyAV CCTTC 5 cut(s) 17, 181, 604, 684, 895
HpyCH4III ACNGT 2 cut(s) 530, 704
HpyCH4V TGCA 4 cut(s) 212, 251, 473, 640
HpyF10VI GCNNNNNNNGC 3 cut(s) 479, 545, 704
HpyF3I CTNAG 3 cut(s) 152, 163, 348
Hsp92II CATG 4 cut(s) 92, 251, 715, 990
HspAI GCGC 1 cut(s) 546
Kpn2I TCCGGA 1 cut(s) 793
Kzo9I GATC 3 cut(s) 214, 220, 613
LmnI GCTCC 3 cut(s) 284, 310, 854
LweI GCATC 4 cut(s) 92, 460, 561, 627
MabI ACCWGGT 1 cut(s) 964
MaeI CTAG 1 cut(s) 465
MaeIII GTNAC 2 cut(s) 431, 941
MalI GATC 3 cut(s) 216, 222, 615
MboI GATC 3 cut(s) 214, 220, 613
MboII GAAGA 4 cut(s) 122, 148, 196, 392
MfeI CAATTG 1 cut(s) 252
MflI RGATCY 2 cut(s) 214, 613
MhlI GDGCHC 2 cut(s) 315, 509
MluCI AATT 3 cut(s) 252, 739, 979
MmeI TCCRAC 1 cut(s) 729
Mph1103I ATGCAT 1 cut(s) 475
MroI TCCGGA 1 cut(s) 793
MslI CAYNNNNRTG 3 cut(s) 93, 122, 246
MspA1I CMGCKG 1 cut(s) 82
MspI CCGG 1 cut(s) 794
MspR9I CCNGG 1 cut(s) 966
MunI CAATTG 1 cut(s) 252
MvaI CCWGG 1 cut(s) 966
MwoI GCNNNNNNNGC 3 cut(s) 479, 545, 704
NdeII GATC 3 cut(s) 214, 220, 613
NlaIII CATG 4 cut(s) 92, 251, 715, 990
NlaIV GGNNCC 4 cut(s) 312, 615, 867, 883
NmuCI GTSAC 2 cut(s) 431, 941
NsiI ATGCAT 1 cut(s) 475
NspI RCATGY 2 cut(s) 92, 251
PaeI GCATGC 1 cut(s) 251
PceI AGGCCT 1 cut(s) 7
PfeI GAWTC 1 cut(s) 319
Psp6I CCWGG 1 cut(s) 964
PspFI CCCAGC 1 cut(s) 78
PspGI CCWGG 1 cut(s) 964
PspN4I GGNNCC 4 cut(s) 312, 615, 867, 883
PspPI GGNCC 2 cut(s) 865, 962
PstI CTGCAG 1 cut(s) 214
PstNI CAGNNNCTG 1 cut(s) 218
PsuI RGATCY 2 cut(s) 214, 613
PvuII CAGCTG 1 cut(s) 82
RsaI GTAC 1 cut(s) 692
RsaNI GTAC 1 cut(s) 691
RseI CAYNNNNRTG 3 cut(s) 93, 122, 246
Sau3AI GATC 3 cut(s) 214, 220, 613
Sau96I GGNCC 2 cut(s) 865, 962
ScrFI CCNGG 1 cut(s) 966
SduI GDGCHC 2 cut(s) 315, 509
SexAI ACCWGGT 1 cut(s) 964
SfaNI GCATC 4 cut(s) 92, 460, 561, 627
SfcI CTRYAG 1 cut(s) 210
SinI GGWCC 1 cut(s) 962
SmiMI CAYNNNNRTG 3 cut(s) 93, 122, 246
SmlI CTYRAG 1 cut(s) 860
SmoI CTYRAG 1 cut(s) 860
SpeI ACTAGT 1 cut(s) 464
SphI GCATGC 1 cut(s) 251
Sse9I AATT 3 cut(s) 252, 739, 979
SseBI AGGCCT 1 cut(s) 7
SsiI CCGC 1 cut(s) 181
SspMI CTAG 1 cut(s) 465
StuI AGGCCT 1 cut(s) 7
StyD4I CCNGG 1 cut(s) 964
TaaI ACNGT 2 cut(s) 530, 704
TaqI TCGA 1 cut(s) 59
TasI AATT 3 cut(s) 252, 739, 979
TfiI GAWTC 1 cut(s) 319
TscAI CASTG 4 cut(s) 490, 535, 709, 787
TseFI GTSAC 2 cut(s) 431, 941
Tsp45I GTSAC 2 cut(s) 431, 941
TspRI CASTG 4 cut(s) 490, 535, 709, 787
VpaK11BI GGWCC 1 cut(s) 962
XapI RAATTY 1 cut(s) 739
XceI RCATGY 2 cut(s) 92, 251
XmiI GTMKAC 1 cut(s) 598
XspI CTAG 1 cut(s) 465
Zsp2I ATGCAT 1 cut(s) 475
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.