Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0004991

Physical Location & Seq

Genomic Coordinates Forward (+)

273800 .. 277531

Gene Structure 3732 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1003400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1254 bp

ATGGAGTTTGCTGGGTGCAGCAGAGGGAGCTCTGGGAGAGCTCTGGCGCTGGCCATTTGCCTATTGACCTTGATCTCTCTATCTTGTGCTGCCAGATTGAGTGCTTCTTCAAGGCAGAAGCTTGATGTCCACAAGCACCTAAACCGCTTGAACAAACCCGCCGTGAAAAGCATCAAGAGCCCAGATGGAGATATTATAGACTGTGTTCATATCTCTCAGCAACCGGCTTTCGATCACCCTTACCTCAAAGATCACAAAATCCAGATGAGGCCTACCTACCACCCCGAAGGGTTATTTGCTGAGAACAAAGTGGCTGAAAGTACAAAAGAAAGGGTAAATACCCAGCTGTGGCATGTCAATGGGAGATGCCCGGAAGATACTATTCCGGTAAGGAGAACGAAGGAAGATGATATTCTGAGAGCAAGTTCAATGAAACATTATGGAAGGAAGAAGCAGAGAACCATACCAAAATCTGCTGATCCTGATCTTGCCAATGAGAGTGGCCATCAGCATGCAATTGCATATGTCAATGGAGATAAGTACTATGGAGCAAAAGCAACCATAAATGTCTGGGAACCCAAGATACAACAGCCTAATGAGTTCAGCTTGTCTCAGCTGTGGGTATTAGGAGGATCTTTTGGTGAAGATCTGAACAGCATTGAAGCTGGCTGGCAGGTAAGCCCAGACCTCTATGGTGACAACAACACAAGACTTTTTACATACTGGACTAGTGATGCATATCAAGCCACTGGTTGCTACAACCTCCTCTGCTCAGGCTTCATTCAAATCAACAATGAAATAGCAATGGGTGCAAGCATCTCTCCAGTTTCAGGCGTTCGAGGATCGCAATATGACATCAGTATACTTGTCTGGAAGGATCCAAAGGAGGGGCACTGGTGGATGCAATTCGGAAACGACTATGTGCTGGGTTATTGGCCTTCTTACCTATTCTCCTACTTGGCTGACAGTGCTTCCATGGTTGAGTGGGGAGGTGAGGTTGTGAATTCAGAGCCGGACGGCCAGCACACCTCAACCCAAATGGGCAGCGGACGTTTTCCAGAGGAGGGTTTTGGCAAGGCAAGTTATTTCAGAAACATCCAGGTTGTTGATAGCTCAAATAATCTCAAGGCCCCAAAAGGGTTGGGCACGTTCACTGAGCAACCAAACTGCTATGATGTTCGAACTGGCAGTAATGGAGACTGGGGTCATTACTTTTACTACGGAGGCCCCGGTAAAAACTCGAATTGCCCGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Neprosin Neprosin_AP

Protein Analysis

418

Amino Acids

46.46

Weight (kDa)

6.5

Isoelectric Point (pI)

38.93

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Neprosin_AP	PF14365	57 - 175	4.8e-48	Neprosin activation peptide
Neprosin	PF03080	199 - 410	3.1e-87	Neprosin

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0004991)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G55360 AT3G13510 AT5G56530 AT5G56530
fragaria_vesca	FvH4_3g45750 FvH4_3g45750 FvH4_3g45750 FvH4_3g45750 FvH4_3g45750 FvH4_3g45750
malus_domestica	MD03G1003500.v1.1 MD11G1003400.v1.1
prunus_persica	Prupe.6G003200_v2.0.a1 Prupe.6G003200_v2.0.a1 Prupe.6G003200_v2.0.a1
pyrus_communis	pycom03g00350 pycom11g00300
rosa_chinensis	RchiOBHm_Chr5g0083821
rosa_laevigata	RLG00000037054
rosa_multiflora	Rmu_sc0024007.1_g000001
rosa_roxburghii	Rroxscaffold_1G00000770
rosa_rugosa	Rorug05G0486300 Rorug05G0486300 Rorug05G0486300 Rorug05G0486300 Rorug05G0486300
rosa_samantha	Rh5AG539300 Rh5CG586500 Rh5DG573000
rosa_wichuraiana	Rw5G050140

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	664
AccI	GTMKAC	1 cut(s)	862
AciI	CCGC	3 cut(s)	145, 159, 1047
AclWI	GGATC	5 cut(s)	473, 640, 850, 872, 885
AcoI	YGGCCR	3 cut(s)	51, 502, 1018
AcsI	RAATTY	1 cut(s)	1003
AfaI	GTAC	2 cut(s)	322, 542
AfiI	CCNNNNNNNGG	5 cut(s)	348, 830, 887, 1064, 1137
AgsI	TTSAA	5 cut(s)	111, 151, 429, 662, 785
AhlI	ACTAGT	1 cut(s)	728
AjnI	CCWGG	1 cut(s)	1098
AluBI	AGCT	8 cut(s)	30, 41, 121, 346, 606, 616, 665, 1113
AluI	AGCT	8 cut(s)	30, 41, 121, 346, 606, 616, 665, 1113
Alw21I	GWGCWC	2 cut(s)	32, 43
Alw26I	GTCTC	2 cut(s)	615, 1191
AlwI	GGATC	5 cut(s)	473, 640, 850, 872, 885
AoxI	GGCC	7 cut(s)	51, 269, 502, 935, 1018, 1128, 1225
ApeKI	GCWGC	3 cut(s)	18, 89, 1044
ApoI	RAATTY	1 cut(s)	1003
AspLEI	GCGC	1 cut(s)	49
AspS9I	GGNCC	2 cut(s)	1129, 1226
AsuC2I	CCSGG	2 cut(s)	371, 1230
AsuHPI	GGTGA	4 cut(s)	227, 653, 707, 1004
AsuII	TTCGAA	1 cut(s)	1180
BaeGI	GKGCMC	2 cut(s)	894, 1148
BalI	TGGCCA	2 cut(s)	53, 504
BamHI	GGATCC	1 cut(s)	877
BanII	GRGCYC	3 cut(s)	32, 43, 182
Bbv12I	GWGCWC	2 cut(s)	32, 43
BbvI	GCAGC	3 cut(s)	30, 76, 1056
BccI	CCATC	2 cut(s)	179, 513
BceAI	ACGGC	2 cut(s)	146, 1033
BciT130I	CCWGG	1 cut(s)	1100
BcnI	CCSGG	2 cut(s)	371, 1230
BcoDI	GTCTC	2 cut(s)	615, 1191
BcuI	ACTAGT	1 cut(s)	728
BfaI	CTAG	1 cut(s)	729
BfoI	RGCGCY	1 cut(s)	50
BfuAI	ACCTGC	1 cut(s)	664
BglII	AGATCT	1 cut(s)	646
BisI	GCNGC	3 cut(s)	19, 90, 1045
BlsI	GCNGC	3 cut(s)	20, 91, 1046
BmcAI	AGTACT	1 cut(s)	542
Bme1390I	CCNGG	3 cut(s)	371, 1100, 1230
BmgT120I	GGNCC	2 cut(s)	1129, 1226
BmiI	GGNNCC	4 cut(s)	576, 879, 1131, 1228
BmrFI	CCNGG	3 cut(s)	371, 1100, 1230
BmrI	ACTGGG	1 cut(s)	1210
BmsI	GCATC	5 cut(s)	180, 356, 724, 825, 891
BmuI	ACTGGG	1 cut(s)	1210
BoxI	GACNNNNGTC	1 cut(s)	1203
BpmI	CTGGAG	1 cut(s)	807
Bpu10I	CCTNAGC	1 cut(s)	772
Bpu14I	TTCGAA	1 cut(s)	1180
BpuEI	CTTGAG	1 cut(s)	1109
BpuMI	CCSGG	2 cut(s)	371, 1230
BsaBI	GATNNNNATC	2 cut(s)	483, 738
BsaJI	CCNNGG	2 cut(s)	975, 1228
BsaWI	WCCGGW	1 cut(s)	385
BsaXI	ACNNNNNCTCC	4 cut(s)	935, 965, 981, 1011
Bsc4I	CCNNNNNNNGG	5 cut(s)	348, 830, 887, 1064, 1137
Bse118I	RCCGGY	1 cut(s)	223
Bse1I	ACTGG	6 cut(s)	728, 754, 824, 899, 1189, 1205
Bse3DI	GCAATG	1 cut(s)	810
Bse8I	GATNNNNATC	2 cut(s)	483, 738
BseBI	CCWGG	1 cut(s)	1100
BseDI	CCNNGG	2 cut(s)	975, 1228
BseGI	GGATG	2 cut(s)	906, 1095
BseJI	GATNNNNATC	2 cut(s)	483, 738
BseLI	CCNNNNNNNGG	5 cut(s)	348, 830, 887, 1064, 1137
BseMI	GCAATG	1 cut(s)	810
BseMII	CTCAG	6 cut(s)	230, 291, 407, 626, 786, 1146
BseNI	ACTGG	6 cut(s)	728, 754, 824, 899, 1189, 1205
BseRI	GAGGAG	2 cut(s)	755, 1076
BseSI	GKGCMC	2 cut(s)	894, 1148
BseXI	GCAGC	3 cut(s)	30, 76, 1056
BseYI	CCCAGC	3 cut(s)	11, 342, 925
BsgI	GTGCAG	1 cut(s)	37
BshFI	GGCC	7 cut(s)	53, 271, 504, 937, 1020, 1130, 1227
BsiHKAI	GWGCWC	2 cut(s)	32, 43
BsiSI	CCGG	5 cut(s)	224, 371, 386, 1013, 1230
BslI	CCNNNNNNNGG	5 cut(s)	348, 830, 887, 1064, 1137
BsmAI	GTCTC	2 cut(s)	615, 1191
BsnI	GGCC	7 cut(s)	53, 271, 504, 937, 1020, 1130, 1227
Bsp119I	TTCGAA	1 cut(s)	1180
Bsp1286I	GDGCHC	5 cut(s)	32, 43, 182, 894, 1148
Bsp143I	GATC	9 cut(s)	72, 232, 250, 478, 484, 632, 646, 842, 877
Bsp19I	CCATGG	1 cut(s)	975
BspACI	CCGC	3 cut(s)	145, 159, 1047
BspANI	GGCC	7 cut(s)	53, 271, 504, 937, 1020, 1130, 1227
BspCNI	CTCAG	6 cut(s)	229, 292, 408, 625, 785, 1147
BspLI	GGNNCC	4 cut(s)	576, 879, 1131, 1228
BspMI	ACCTGC	1 cut(s)	664
BspPI	GGATC	5 cut(s)	473, 640, 850, 872, 885
BspT104I	TTCGAA	1 cut(s)	1180
BsrDI	GCAATG	1 cut(s)	810
BsrFI	RCCGGY	1 cut(s)	223
BsrI	ACTGG	6 cut(s)	728, 754, 824, 899, 1189, 1205
BssAI	RCCGGY	1 cut(s)	223
BssECI	CCNNGG	2 cut(s)	975, 1228
BssMI	GATC	9 cut(s)	72, 232, 250, 478, 484, 632, 646, 842, 877
BssNAI	GTATAC	1 cut(s)	863
BssT1I	CCWWGG	1 cut(s)	975
Bst1107I	GTATAC	1 cut(s)	863
Bst2UI	CCWGG	1 cut(s)	1100
Bst4CI	ACNGT	2 cut(s)	203, 968
BstAPI	GCANNNNNTGC	1 cut(s)	809
BstBI	TTCGAA	1 cut(s)	1180
BstC8I	GCNNGC	6 cut(s)	51, 513, 667, 671, 814, 1022
BstDEI	CTNAG	6 cut(s)	216, 300, 416, 612, 772, 1155
BstDSI	CCRYGG	1 cut(s)	975
BstF5I	GGATG	2 cut(s)	906, 1095
BstH2I	RGCGCY	1 cut(s)	50
BstHHI	GCGC	1 cut(s)	49
BstKTI	GATC	9 cut(s)	75, 235, 253, 481, 487, 635, 649, 845, 880
BstMAI	GTCTC	2 cut(s)	615, 1191
BstMBI	GATC	9 cut(s)	72, 232, 250, 478, 484, 632, 646, 842, 877
BstMWI	GCNNNNNNNGC	5 cut(s)	27, 177, 743, 809, 968
BstNI	CCWGG	1 cut(s)	1100
BstNSI	RCATGY	2 cut(s)	356, 515
BstPAI	GACNNNNGTC	1 cut(s)	1203
BstSCI	CCNGG	3 cut(s)	369, 1098, 1228
BstSLI	GKGCMC	2 cut(s)	894, 1148
BstV1I	GCAGC	3 cut(s)	30, 76, 1056
BstX2I	RGATCY	3 cut(s)	632, 646, 877
BstYI	RGATCY	3 cut(s)	632, 646, 877
BstZ17I	GTATAC	1 cut(s)	863
BsuRI	GGCC	7 cut(s)	53, 271, 504, 937, 1020, 1130, 1227
BtgI	CCRYGG	1 cut(s)	975
BtsCI	GGATG	2 cut(s)	906, 1095
BtsIMutI	CAGTG	4 cut(s)	747, 892, 973, 1152
BveI	ACCTGC	1 cut(s)	664
Cac8I	GCNNGC	6 cut(s)	51, 513, 667, 671, 814, 1022
CfoI	GCGC	1 cut(s)	49
Cfr10I	RCCGGY	1 cut(s)	223
Cfr13I	GGNCC	2 cut(s)	1129, 1226
Csp6I	GTAC	2 cut(s)	321, 541
CspCI	CAANNNNNGTGG	2 cut(s)	481, 516
CviAII	CATG	3 cut(s)	353, 512, 976
CviQI	GTAC	2 cut(s)	321, 541
DdeI	CTNAG	6 cut(s)	216, 300, 416, 612, 772, 1155
DpnI	GATC	9 cut(s)	74, 234, 252, 480, 486, 634, 648, 844, 879
DpnII	GATC	9 cut(s)	72, 232, 250, 478, 484, 632, 646, 842, 877
EaeI	YGGCCR	3 cut(s)	51, 502, 1018
Ecl136II	GAGCTC	2 cut(s)	30, 41
Eco130I	CCWWGG	1 cut(s)	975
Eco147I	AGGCCT	1 cut(s)	271
Eco24I	GRGCYC	3 cut(s)	32, 43, 182
Eco53kI	GAGCTC	2 cut(s)	30, 41
EcoICRI	GAGCTC	2 cut(s)	30, 41
EcoO109I	RGGNCCY	2 cut(s)	1129, 1226
EcoRI	GAATTC	1 cut(s)	1003
EcoRII	CCWGG	1 cut(s)	1098
EcoT14I	CCWWGG	1 cut(s)	975
EcoT22I	ATGCAT	1 cut(s)	739
EcoT38I	GRGCYC	3 cut(s)	32, 43, 182
ErhI	CCWWGG	1 cut(s)	975
FaeI	CATG	3 cut(s)	356, 515, 979
FatI	CATG	3 cut(s)	352, 511, 975
FauI	CCCGC	1 cut(s)	166
FauNDI	CATATG	1 cut(s)	523
FblI	GTMKAC	1 cut(s)	862
Fnu4HI	GCNGC	3 cut(s)	19, 90, 1045
FokI	GGATG	2 cut(s)	913, 1082
FriOI	GRGCYC	3 cut(s)	32, 43, 182
Fsp4HI	GCNGC	3 cut(s)	19, 90, 1045
FspBI	CTAG	1 cut(s)	729
GlaI	GCGC	1 cut(s)	48
GluI	GCNGC	3 cut(s)	19, 90, 1045
GsaI	CCCAGC	3 cut(s)	15, 346, 929
GsuI	CTGGAG	1 cut(s)	807
HaeII	RGCGCY	1 cut(s)	50
HaeIII	GGCC	7 cut(s)	53, 271, 504, 937, 1020, 1130, 1227
HapII	CCGG	5 cut(s)	224, 371, 386, 1013, 1230
HhaI	GCGC	1 cut(s)	49
Hin1II	CATG	3 cut(s)	356, 515, 979
Hin6I	GCGC	1 cut(s)	47
HinP1I	GCGC	1 cut(s)	47
HindIII	AAGCTT	1 cut(s)	119
HpaII	CCGG	5 cut(s)	224, 371, 386, 1013, 1230
HphI	GGTGA	4 cut(s)	227, 653, 707, 1004
Hpy166II	GTNNAC	3 cut(s)	130, 863, 1152
Hpy188I	TCNGA	5 cut(s)	417, 651, 911, 1009, 1091
Hpy188III	TCNNGA	5 cut(s)	175, 262, 482, 871, 1058
Hpy8I	GTNNAC	3 cut(s)	130, 863, 1152
HpyAV	CCTTC	5 cut(s)	281, 394, 438, 868, 948
HpyCH4III	ACNGT	2 cut(s)	203, 968
HpyCH4IV	ACGT	2 cut(s)	1051, 1148
HpyCH4V	TGCA	6 cut(s)	18, 515, 521, 737, 812, 904
HpyF10VI	GCNNNNNNNGC	5 cut(s)	27, 177, 743, 809, 968
HpyF3I	CTNAG	6 cut(s)	216, 300, 416, 612, 772, 1155
HpySE526I	ACGT	2 cut(s)	1051, 1148
Hsp92II	CATG	3 cut(s)	356, 515, 979
HspAI	GCGC	1 cut(s)	47
Kzo9I	GATC	9 cut(s)	72, 232, 250, 478, 484, 632, 646, 842, 877
LmnI	GCTCC	2 cut(s)	27, 548
Lsp1109I	GCAGC	3 cut(s)	30, 76, 1056
LweI	GCATC	5 cut(s)	180, 356, 724, 825, 891
MaeI	CTAG	1 cut(s)	729
MaeII	ACGT	2 cut(s)	1051, 1148
MaeIII	GTNAC	1 cut(s)	695
MalI	GATC	9 cut(s)	74, 234, 252, 480, 486, 634, 648, 844, 879
MboI	GATC	9 cut(s)	72, 232, 250, 478, 484, 632, 646, 842, 877
MboII	GAAGA	5 cut(s)	99, 386, 416, 460, 656
MfeI	CAATTG	1 cut(s)	516
MflI	RGATCY	3 cut(s)	632, 646, 877
MhlI	GDGCHC	5 cut(s)	32, 43, 182, 894, 1148
MlsI	TGGCCA	2 cut(s)	53, 504
MluCI	AATT	4 cut(s)	516, 905, 1003, 1243
MluNI	TGGCCA	2 cut(s)	53, 504
Mox20I	TGGCCA	2 cut(s)	53, 504
Mph1103I	ATGCAT	1 cut(s)	739
MscI	TGGCCA	2 cut(s)	53, 504
MslI	CAYNNNNRTG	2 cut(s)	357, 510
Msp20I	TGGCCA	2 cut(s)	53, 504
MspA1I	CMGCKG	3 cut(s)	346, 616, 1047
MspI	CCGG	5 cut(s)	224, 371, 386, 1013, 1230
MspR9I	CCNGG	3 cut(s)	371, 1100, 1230
MunI	CAATTG	1 cut(s)	516
MvaI	CCWGG	1 cut(s)	1100
MwoI	GCNNNNNNNGC	5 cut(s)	27, 177, 743, 809, 968
NciI	CCSGG	2 cut(s)	371, 1230
NcoI	CCATGG	1 cut(s)	975
NdeI	CATATG	1 cut(s)	523
NdeII	GATC	9 cut(s)	72, 232, 250, 478, 484, 632, 646, 842, 877
NlaIII	CATG	3 cut(s)	356, 515, 979
NlaIV	GGNNCC	4 cut(s)	576, 879, 1131, 1228
NmuCI	GTSAC	1 cut(s)	695
NsiI	ATGCAT	1 cut(s)	739
NspI	RCATGY	2 cut(s)	356, 515
NspV	TTCGAA	1 cut(s)	1180
PaeI	GCATGC	1 cut(s)	515
PceI	AGGCCT	1 cut(s)	271
PcsI	WCGNNNNNNNCGW	1 cut(s)	1247
PkrI	GCNGC	3 cut(s)	20, 91, 1046
PshAI	GACNNNNGTC	1 cut(s)	1203
Psp124BI	GAGCTC	2 cut(s)	32, 43
Psp6I	CCWGG	1 cut(s)	1098
PspFI	CCCAGC	3 cut(s)	11, 342, 925
PspGI	CCWGG	1 cut(s)	1098
PspN4I	GGNNCC	4 cut(s)	576, 879, 1131, 1228
PspPI	GGNCC	2 cut(s)	1129, 1226
PsrI	GAACNNNNNNTAC	2 cut(s)	567, 599
PsuI	RGATCY	3 cut(s)	632, 646, 877
PvuII	CAGCTG	2 cut(s)	346, 616
RsaI	GTAC	2 cut(s)	322, 542
RsaNI	GTAC	2 cut(s)	321, 541
RseI	CAYNNNNRTG	2 cut(s)	357, 510
SacI	GAGCTC	2 cut(s)	32, 43
SatI	GCNGC	3 cut(s)	19, 90, 1045
Sau3AI	GATC	9 cut(s)	72, 232, 250, 478, 484, 632, 646, 842, 877
Sau96I	GGNCC	2 cut(s)	1129, 1226
ScaI	AGTACT	1 cut(s)	542
ScrFI	CCNGG	3 cut(s)	371, 1100, 1230
SduI	GDGCHC	5 cut(s)	32, 43, 182, 894, 1148
SfaNI	GCATC	5 cut(s)	180, 356, 724, 825, 891
SfuI	TTCGAA	1 cut(s)	1180
SmiMI	CAYNNNNRTG	2 cut(s)	357, 510
SmlI	CTYRAG	1 cut(s)	1124
SmoI	CTYRAG	1 cut(s)	1124
SpeI	ACTAGT	1 cut(s)	728
SphI	GCATGC	1 cut(s)	515
Sse9I	AATT	4 cut(s)	516, 905, 1003, 1243
SseBI	AGGCCT	1 cut(s)	271
SsiI	CCGC	3 cut(s)	145, 159, 1047
SspMI	CTAG	1 cut(s)	729
SstI	GAGCTC	2 cut(s)	32, 43
StuI	AGGCCT	1 cut(s)	271
StyD4I	CCNGG	3 cut(s)	369, 1098, 1228
StyI	CCWWGG	1 cut(s)	975
TaaI	ACNGT	2 cut(s)	203, 968
TaiI	ACGT	2 cut(s)	1054, 1151
TaqI	TCGA	4 cut(s)	231, 838, 1180, 1241
TasI	AATT	4 cut(s)	516, 905, 1003, 1243
TatI	WGTACW	2 cut(s)	320, 540
TscAI	CASTG	4 cut(s)	754, 899, 973, 1159
TseFI	GTSAC	1 cut(s)	695
TseI	GCWGC	3 cut(s)	18, 89, 1044
Tsp45I	GTSAC	1 cut(s)	695
TspDTI	ATGAA	4 cut(s)	197, 446, 769, 810
TspGWI	ACGGA	1 cut(s)	1236
TspRI	CASTG	4 cut(s)	754, 899, 973, 1159
XapI	RAATTY	1 cut(s)	1003
XceI	RCATGY	2 cut(s)	356, 515
XmiI	GTMKAC	1 cut(s)	862
XspI	CTAG	1 cut(s)	729
ZrmI	AGTACT	1 cut(s)	542
Zsp2I	ATGCAT	1 cut(s)	739

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.