AT5G57140

purple acid phosphatase 28

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0015175
Physical Location & Seq
Forward (+)
23149880 .. 23151677
1798 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G57140.1

Sequence Viewer

Length: 1194 bp
ATGAATTGTTCAATCGGAAACTGGAAACACACTGTTCTCTACTTAACCCTAATCGTATCTCTTCTCTACTTCATCGAATCCCTAATCTCACACAAATTACACATCAACTACAACAAAATCCGCCTGAAAAGATCTCCGAATCTTCCTCTACGATTCCGTGACGATGGCACATTCAAAATCCTCCAGGTCGCGGATATGCATTTCGGAATGGGGATGATTACTCGATGTAGAGATGTGTTGGATTCAGAATTCGAATATTGTTCTGATCTCAACACTACTCGGTTTCTTCGTAGGATGATTGAATCTGAGAGACCTGATTTGATTGCTTTCACTGGGGATAATATATTTGGATCAAGTACTACTGATGCAGCTGAATCTCTTCTTGAAGCTATTGGTCCAGCTATTGAATATGGAATCCCATGGGCTGCTGTTTTAGGAAATCATGACCATGAATCTACTTTGAACCGTTTAGAATTGATGACTTTCCTTTCTCTTATGGATTTTTCTGTTTCTCAAATCAATCCACTTGTTGAAGATGAAACTAAAGGAGATACAATGAGGTTAATCGATGGATTTGGGAATTACCGAGTGAGAGTTTACGGTGCTCCTGGTTCTGTGTTGGCGAATAGTACCGTCTTTGATCTCTTCTTTTTTGATAGTGGAGATAGAGAAATAGTACAAGGTAAACGAACTTATGGATGGATCAAGGAATCACAACTTCGTTGGCTTCAAGATACTTCTATACAGGGTCATAGCCAGAGGATTCATGTTAATCCGCCAGCGTTAGCCTTCTTCCACATCCCAATATTGGAAGTTCGGGAACTGTGGTACACGCCCTTTATCGGTCAGTTTCAGGAAGGAGTGGCATGCTCTATTGTTCAGTCTGGAGTCTTACAGACATTTGTGTCAATGGGAAATGTAAAAGCTGCGTTCATGGGACACGACCATGTTAATGATTTCTGTGGAACTCTAAAAGGGGTATGGTTTTGTTACGGTGGAGGATTCGGATACCATGCTTATGGACGACCAAATTGGCATAGAAGAGCAAGAGTGATAGAAGCTAAGCTTGGGAAAGGAAGAGACACATGGGAAGGAATCAAACTTATCAAGACTTGGAAACGTCTTGACGACGAATACTTGAGCAAAATAGACGAACAAGTCTTATGGGAAACTTCCGACTCATTTCTGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003993 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009987 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0042578 GO:0044237
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos

Protein Analysis

397

Amino Acids

45.44

Weight (kDa)

6.24

Isoelectric Point (pI)

40.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos PF00149 58 - 317 2.8e-14 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015175)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G57140
fragaria_vesca FvH4_4g07080
malus_domestica MD04G1168800.v1.1
prunus_persica Prupe.1G074200_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0399201
rosa_laevigata RLG00000009290
rosa_multiflora Rmu_co8469863.1_g000001 Rmu_sc0002582.1_g000004 Rmu_sc0024868.1_g000004
rosa_roxburghii Rroxscaffold_5G00344160
rosa_rugosa Rorug04G0015100
rosa_samantha Rh4AG093000 Rh4BG090800 Rh4CG101400 Rh4DG085300
rosa_wichuraiana Rw4G007660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 904, 1157
AccII CGCG 1 cut(s) 191
AciI CCGC 3 cut(s) 121, 191, 776
AclWI GGATC 2 cut(s) 358, 710
AcsI RAATTY 1 cut(s) 248
AfaI GTAC 4 cut(s) 358, 631, 678, 830
AfiI CCNNNNNNNGG 3 cut(s) 190, 808, 842
AgsI TTSAA 8 cut(s) 12, 175, 302, 386, 407, 463, 533, 731
AjnI CCWGG 2 cut(s) 183, 607
AjuI GAANNNNNNNTTGG 2 cut(s) 1050, 1082
AluBI AGCT 6 cut(s) 371, 389, 401, 926, 1061, 1066
AluI AGCT 6 cut(s) 371, 389, 401, 926, 1061, 1066
Alw21I GWGCWC 1 cut(s) 607
Alw26I GTCTC 2 cut(s) 304, 1074
AlwI GGATC 2 cut(s) 358, 710
ApeKI GCWGC 3 cut(s) 368, 425, 926
ApoI RAATTY 1 cut(s) 248
Asp700I GAANNNNTTC 1 cut(s) 378
AspS9I GGNCC 1 cut(s) 395
AsuII TTCGAA 1 cut(s) 252
AvaII GGWCC 1 cut(s) 395
Bbv12I GWGCWC 1 cut(s) 607
BbvI GCAGC 3 cut(s) 380, 412, 913
BccI CCATC 3 cut(s) 158, 563, 693
BciT130I CCWGG 2 cut(s) 185, 609
BciVI GTATCC 1 cut(s) 1001
BcoDI GTCTC 2 cut(s) 304, 1074
BfuI GTATCC 1 cut(s) 1001
BglII AGATCT 1 cut(s) 131
BisI GCNGC 3 cut(s) 369, 426, 927
BlpI GCTNAGC 1 cut(s) 1062
BlsI GCNGC 3 cut(s) 370, 427, 928
BmcAI AGTACT 1 cut(s) 358
Bme1390I CCNGG 2 cut(s) 185, 609
Bme18I GGWCC 1 cut(s) 395
BmgT120I GGNCC 1 cut(s) 395
BmrFI CCNGG 2 cut(s) 185, 609
BmrI ACTGGG 1 cut(s) 342
BmsI GCATC 1 cut(s) 355
BmuI ACTGGG 1 cut(s) 342
BpmI CTGGAG 2 cut(s) 167, 906
Bpu1102I GCTNAGC 1 cut(s) 1062
Bpu14I TTCGAA 1 cut(s) 252
BpuEI CTTGAG 1 cut(s) 1159
Bsa29I ATCGAT 1 cut(s) 567
BsaI GGTCTC 1 cut(s) 304
BsaJI CCNNGG 1 cut(s) 419
Bsc4I CCNNNNNNNGG 3 cut(s) 190, 808, 842
Bse1I ACTGG 2 cut(s) 26, 337
BseBI CCWGG 2 cut(s) 185, 609
BseCI ATCGAT 1 cut(s) 567
BseDI CCNNGG 1 cut(s) 419
BseGI GGATG 4 cut(s) 219, 300, 704, 798
BseLI CCNNNNNNNGG 3 cut(s) 190, 808, 842
BseMII CTCAG 1 cut(s) 297
BseNI ACTGG 2 cut(s) 26, 337
BseXI GCAGC 3 cut(s) 380, 412, 913
Bsh1236I CGCG 1 cut(s) 191
BshVI ATCGAT 1 cut(s) 567
BsiHKAI GWGCWC 1 cut(s) 607
BslFI GGGAC 1 cut(s) 951
BslI CCNNNNNNNGG 3 cut(s) 190, 808, 842
BsmAI GTCTC 2 cut(s) 304, 1074
BsmFI GGGAC 1 cut(s) 951
Bso31I GGTCTC 1 cut(s) 304
Bsp119I TTCGAA 1 cut(s) 252
Bsp1286I GDGCHC 1 cut(s) 607
Bsp143I GATC 5 cut(s) 131, 265, 350, 640, 702
Bsp1720I GCTNAGC 1 cut(s) 1062
Bsp19I CCATGG 1 cut(s) 419
BspACI CCGC 3 cut(s) 121, 191, 776
BspCNI CTCAG 1 cut(s) 298
BspDI ATCGAT 1 cut(s) 567
BspFNI CGCG 1 cut(s) 191
BspHI TCATGA 1 cut(s) 442
BspPI GGATC 2 cut(s) 358, 710
BspQI GCTCTTC 1 cut(s) 1036
BspT104I TTCGAA 1 cut(s) 252
BspTNI GGTCTC 1 cut(s) 304
BsrI ACTGG 2 cut(s) 26, 337
BssECI CCNNGG 1 cut(s) 419
BssMI GATC 5 cut(s) 131, 265, 350, 640, 702
BssT1I CCWWGG 1 cut(s) 419
Bst2UI CCWGG 2 cut(s) 185, 609
Bst4CI ACNGT 6 cut(s) 34, 467, 602, 634, 825, 995
Bst6I CTCTTC 5 cut(s) 66, 384, 650, 1036, 1072
BstBI TTCGAA 1 cut(s) 252
BstC8I GCNNGC 2 cut(s) 780, 868
BstDEI CTNAG 2 cut(s) 306, 1062
BstDSI CCRYGG 1 cut(s) 419
BstF5I GGATG 4 cut(s) 219, 300, 704, 798
BstFNI CGCG 1 cut(s) 191
BstKTI GATC 5 cut(s) 134, 268, 353, 643, 705
BstMAI GTCTC 2 cut(s) 304, 1074
BstMBI GATC 5 cut(s) 131, 265, 350, 640, 702
BstNI CCWGG 2 cut(s) 185, 609
BstNSI RCATGY 1 cut(s) 870
BstSCI CCNGG 2 cut(s) 183, 607
BstUI CGCG 1 cut(s) 191
BstV1I GCAGC 3 cut(s) 380, 412, 913
BstX2I RGATCY 1 cut(s) 131
BstXI CCANNNNNNTGG 1 cut(s) 1019
BstYI RGATCY 1 cut(s) 131
Bsu15I ATCGAT 1 cut(s) 567
BsuI GTATCC 1 cut(s) 1001
BsuTUI ATCGAT 1 cut(s) 567
BtgI CCRYGG 1 cut(s) 419
BtsCI GGATG 4 cut(s) 219, 300, 704, 798
BtsIMutI CAGTG 2 cut(s) 30, 330
Cac8I GCNNGC 2 cut(s) 780, 868
CciI TCATGA 1 cut(s) 442
Cfr13I GGNCC 1 cut(s) 395
ClaI ATCGAT 1 cut(s) 567
Csp6I GTAC 4 cut(s) 357, 630, 677, 829
CviAII CATG 9 cut(s) 420, 443, 449, 767, 867, 934, 947, 1013, 1086
CviQI GTAC 4 cut(s) 357, 630, 677, 829
DdeI CTNAG 2 cut(s) 306, 1062
DpnI GATC 5 cut(s) 133, 267, 352, 642, 704
DpnII GATC 5 cut(s) 131, 265, 350, 640, 702
DrdI GACNNNNNNGTC 2 cut(s) 904, 1157
DseDI GACNNNNNNGTC 2 cut(s) 904, 1157
Eam1104I CTCTTC 5 cut(s) 66, 384, 650, 1036, 1072
EarI CTCTTC 5 cut(s) 66, 384, 650, 1036, 1072
EciI GGCGGA 2 cut(s) 110, 765
Eco130I CCWWGG 1 cut(s) 419
Eco31I GGTCTC 1 cut(s) 304
Eco47I GGWCC 1 cut(s) 395
EcoRI GAATTC 1 cut(s) 248
EcoRII CCWGG 2 cut(s) 183, 607
EcoT14I CCWWGG 1 cut(s) 419
EcoT22I ATGCAT 1 cut(s) 201
ErhI CCWWGG 1 cut(s) 419
FaeI CATG 9 cut(s) 423, 446, 452, 770, 870, 937, 950, 1016, 1089
FalI AAGNNNNNCTT 2 cut(s) 1050, 1082
FaqI GGGAC 1 cut(s) 951
FatI CATG 9 cut(s) 419, 442, 448, 766, 866, 933, 946, 1012, 1085
Fnu4HI GCNGC 3 cut(s) 369, 426, 927
FokI GGATG 4 cut(s) 226, 307, 711, 785
Fsp4HI GCNGC 3 cut(s) 369, 426, 927
GluI GCNGC 3 cut(s) 369, 426, 927
GsuI CTGGAG 2 cut(s) 167, 906
Hin1II CATG 9 cut(s) 423, 446, 452, 770, 870, 937, 950, 1016, 1089
HindIII AAGCTT 1 cut(s) 1064
Hpy166II GTNNAC 3 cut(s) 598, 686, 831
Hpy188I TCNGA 9 cut(s) 17, 138, 206, 247, 265, 307, 1007, 1177, 1188
Hpy188III TCNNGA 8 cut(s) 383, 443, 731, 818, 854, 885, 1108, 1124
Hpy8I GTNNAC 3 cut(s) 598, 686, 831
Hpy99I CGWCG 1 cut(s) 1133
HpyAV CCTTC 3 cut(s) 799, 851, 1085
HpyCH4III ACNGT 6 cut(s) 34, 467, 602, 634, 825, 995
HpyCH4IV ACGT 1 cut(s) 1120
HpyCH4V TGCA 2 cut(s) 199, 368
HpyF3I CTNAG 2 cut(s) 306, 1062
HpySE526I ACGT 1 cut(s) 1120
Hsp92II CATG 9 cut(s) 423, 446, 452, 770, 870, 937, 950, 1016, 1089
Kzo9I GATC 5 cut(s) 131, 265, 350, 640, 702
LguI GCTCTTC 1 cut(s) 1036
LmnI GCTCC 1 cut(s) 610
Lsp1109I GCAGC 3 cut(s) 380, 412, 913
LweI GCATC 1 cut(s) 355
MaeII ACGT 1 cut(s) 1120
MaeIII GTNAC 2 cut(s) 158, 989
MalI GATC 5 cut(s) 133, 267, 352, 642, 704
MboI GATC 5 cut(s) 131, 265, 350, 640, 702
MboII GAAGA 9 cut(s) 53, 134, 278, 371, 545, 637, 784, 1053, 1089
MflI RGATCY 1 cut(s) 131
MhlI GDGCHC 1 cut(s) 607
MluCI AATT 6 cut(s) 4, 95, 248, 473, 580, 1030
MlyI GAGTC 2 cut(s) 897, 1172
MmeI TCCRAC 1 cut(s) 219
MnlI CCTC 5 cut(s) 156, 191, 552, 753, 992
Mph1103I ATGCAT 1 cut(s) 201
MroXI GAANNNNTTC 1 cut(s) 378
MseI TTAA 4 cut(s) 44, 563, 771, 951
MslI CAYNNNNRTG 4 cut(s) 447, 945, 951, 1017
MspA1I CMGCKG 1 cut(s) 371
MspR9I CCNGG 2 cut(s) 185, 609
MvaI CCWGG 2 cut(s) 185, 609
MvnI CGCG 1 cut(s) 191
NcoI CCATGG 1 cut(s) 419
NdeII GATC 5 cut(s) 131, 265, 350, 640, 702
NlaIII CATG 9 cut(s) 423, 446, 452, 770, 870, 937, 950, 1016, 1089
NmuCI GTSAC 1 cut(s) 158
NsiI ATGCAT 1 cut(s) 201
NspI RCATGY 1 cut(s) 870
NspV TTCGAA 1 cut(s) 252
PaeI GCATGC 1 cut(s) 870
PagI TCATGA 1 cut(s) 442
PciSI GCTCTTC 1 cut(s) 1036
PcsI WCGNNNNNNNCGW 1 cut(s) 286
PdmI GAANNNNTTC 1 cut(s) 378
PkrI GCNGC 3 cut(s) 370, 427, 928
PleI GAGTC 2 cut(s) 896, 1172
PpsI GAGTC 2 cut(s) 896, 1172
Psp6I CCWGG 2 cut(s) 183, 607
PspGI CCWGG 2 cut(s) 183, 607
PspPI GGNCC 1 cut(s) 395
PsuI RGATCY 1 cut(s) 131
PvuII CAGCTG 1 cut(s) 371
RsaI GTAC 4 cut(s) 358, 631, 678, 830
RsaNI GTAC 4 cut(s) 357, 630, 677, 829
RseI CAYNNNNRTG 4 cut(s) 447, 945, 951, 1017
SapI GCTCTTC 1 cut(s) 1036
SaqAI TTAA 4 cut(s) 44, 563, 771, 951
SatI GCNGC 3 cut(s) 369, 426, 927
Sau3AI GATC 5 cut(s) 131, 265, 350, 640, 702
Sau96I GGNCC 1 cut(s) 395
ScaI AGTACT 1 cut(s) 358
SchI GAGTC 2 cut(s) 897, 1172
ScrFI CCNGG 2 cut(s) 185, 609
SduI GDGCHC 1 cut(s) 607
SfaNI GCATC 1 cut(s) 355
SfuI TTCGAA 1 cut(s) 252
SinI GGWCC 1 cut(s) 395
SmiMI CAYNNNNRTG 4 cut(s) 447, 945, 951, 1017
SmlI CTYRAG 1 cut(s) 1138
SmoI CTYRAG 1 cut(s) 1138
SphI GCATGC 1 cut(s) 870
Sse9I AATT 6 cut(s) 4, 95, 248, 473, 580, 1030
SsiI CCGC 3 cut(s) 121, 191, 776
SspI AATATT 2 cut(s) 257, 807
StyD4I CCNGG 2 cut(s) 183, 607
StyI CCWWGG 1 cut(s) 419
TaaI ACNGT 6 cut(s) 34, 467, 602, 634, 825, 995
TaiI ACGT 1 cut(s) 1123
TaqI TCGA 4 cut(s) 75, 223, 252, 567
TaqII GACCGA 1 cut(s) 833
TasI AATT 6 cut(s) 4, 95, 248, 473, 580, 1030
TatI WGTACW 2 cut(s) 356, 676
Tru1I TTAA 4 cut(s) 44, 563, 771, 951
Tru9I TTAA 4 cut(s) 44, 563, 771, 951
TscAI CASTG 2 cut(s) 37, 337
TseFI GTSAC 1 cut(s) 158
TseI GCWGC 3 cut(s) 368, 425, 926
Tsp45I GTSAC 1 cut(s) 158
TspDTI ATGAA 6 cut(s) 17, 61, 465, 552, 755, 922
TspGWI ACGGA 1 cut(s) 146
TspRI CASTG 2 cut(s) 37, 337
VpaK11BI GGWCC 1 cut(s) 395
XapI RAATTY 1 cut(s) 248
XceI RCATGY 1 cut(s) 870
XmnI GAANNNNTTC 1 cut(s) 378
ZrmI AGTACT 1 cut(s) 358
Zsp2I ATGCAT 1 cut(s) 201
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.