Rh4DG085300
NAC Family

inactive purple acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0015175
Physical Location & Seq
Reverse (-)
12963566 .. 12966257
2692 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG085300.1

Sequence Viewer

Length: 1047 bp
ATGGGTGGGTTGAGAATTAATGTATTGCAGGTTGCTGATATGCATTATGGGAATGGAGGGTTGACTCGGTGTCGAGACGTATTGGACTCCCAGTTTGCGGGATGTTCTGATCGGAACACGTCGCTGTTCCTCAAGAGGATGATTGAAGCTGAAAAGCCTGATTTCATTGCTTTTACAGGTGATAATATATTTGGGCCAAGCTCTACCGACGCTGCTGAATCAATGATTCGAGCATTTGGTCCTGCCATTAAATCAGGCATTCCGTGGGCAGCAGTTTTGGGAAACCACGACCAAGAATCTACAATGAATCGTGCAGAACTCATGTCCTTTATCTCCCTTATGGACTATTCACTTTCACAAGTTAATCCATTAGCTGAAGATCCCTTAGGAGAATGGATGCAAGACATTGATGGCTTTGGCAATTATAATCTGAGAGTATATGGTGCCCCAGGTTCCTACTTGGCAAATAGCAGCATCCTCAACCTTTTCTTTCTTGACAGTGGAGACAGAGAGACTGTTCATGGAGTACGAACTTACAGTTGGATTAAGGAATCACAACTCCGTTGGCTTCGTGGTGTTTCTCAGGGCCACAGTCGAGATCCAGATCACTCAGACTATGCTTTTTCTCCAGATAAACCACCGGTGCTTGCTTTTTTCCATATCCCAATTCCTGAGATCCGGCAGCTGTGGAATAAAGACATTGTTGGCAAGTTTCAGGAGGCTGTGGCATGTTCATCAGTGAACCCGGGAGTATTGCAGACCCTTGTATCCATGGGAGATGTGAAAGCTGTGTTCATTGGACATGATCATACCAATGACTTCTGTGGGAATCTTGATGGTGTTTGGTTCTGTTATGGTGGGGGCTTTGGATATCATGGTTATGGAAAGAGGGGGTGGCCAAGAAGAGCTAGGGTCATATTGGCAGAACTCGGGAAAGGGGAGAAAGGCTGGATGGGAGTTGAGAGGATTAGGACATGGAAGCATCTTGATGATGAGAAACTGAGCAAGATTGATGAGCAGTTCTTATGGGAATACCATCCATCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003993 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009987 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0042578 GO:0044237
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos

Protein Analysis

348

Amino Acids

38.97

Weight (kDa)

5.66

Isoelectric Point (pI)

38.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos PF00149 7 - 271 1.7e-15 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015175)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G57140
fragaria_vesca FvH4_4g07080
malus_domestica MD04G1168800.v1.1
prunus_persica Prupe.1G074200_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0399201
rosa_laevigata RLG00000009290
rosa_multiflora Rmu_co8469863.1_g000001 Rmu_sc0002582.1_g000004 Rmu_sc0024868.1_g000004
rosa_roxburghii Rroxscaffold_5G00344160
rosa_rugosa Rorug04G0015100
rosa_samantha Rh4AG093000 Rh4BG090800 Rh4CG101400 Rh4DG085300
rosa_wichuraiana Rw4G007660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 426
Acc36I ACCTGC 1 cut(s) 19
AccB1I GGYRCC 1 cut(s) 443
AciI CCGC 1 cut(s) 98
AclWI GGATC 3 cut(s) 374, 593, 670
AcoI YGGCCR 1 cut(s) 896
AcuI CTGAAG 1 cut(s) 396
AfaI GTAC 1 cut(s) 528
AfiI CCNNNNNNNGG 1 cut(s) 97
AflIII ACRYGT 1 cut(s) 117
AgeI ACCGGT 1 cut(s) 640
AgsI TTSAA 1 cut(s) 146
AhdI GACNNNNNGTC 1 cut(s) 69
AjiI CACGTC 1 cut(s) 120
AjnI CCWGG 1 cut(s) 448
AjuI GAANNNNNNNTTGG 2 cut(s) 523, 555
AluBI AGCT 6 cut(s) 149, 201, 374, 685, 788, 908
AluI AGCT 6 cut(s) 149, 201, 374, 685, 788, 908
Alw26I GTCTC 3 cut(s) 69, 498, 506
AlwI GGATC 3 cut(s) 374, 593, 670
Ama87I CYCGRG 2 cut(s) 745, 929
AoxI GGCC 3 cut(s) 194, 586, 896
ApeKI GCWGC 4 cut(s) 212, 269, 471, 682
ArsI GACNNNNNNTTYG 2 cut(s) 77, 109
AseI ATTAAT 1 cut(s) 18
AsiGI ACCGGT 1 cut(s) 640
AspS9I GGNCC 3 cut(s) 194, 239, 586
AsuC2I CCSGG 2 cut(s) 746, 747
AsuHPI GGTGA 1 cut(s) 191
AvaI CYCGRG 2 cut(s) 745, 929
AvaII GGWCC 1 cut(s) 239
AxyI CCTNAGG 1 cut(s) 385
BaeGI GKGCMC 1 cut(s) 448
BaeI ACNNNNGTAYC 2 cut(s) 750, 783
BalI TGGCCA 1 cut(s) 898
BanI GGYRCC 1 cut(s) 443
BbvI GCAGC 4 cut(s) 199, 281, 483, 694
BccI CCATC 4 cut(s) 404, 830, 946, 1044
BciT130I CCWGG 1 cut(s) 450
BciVI GTATCC 1 cut(s) 778
BclI TGATCA 1 cut(s) 805
BcnI CCSGG 2 cut(s) 746, 747
BcoDI GTCTC 3 cut(s) 69, 498, 506
BfaI CTAG 1 cut(s) 909
BfuAI ACCTGC 1 cut(s) 19
BfuI GTATCC 1 cut(s) 778
BisI GCNGC 4 cut(s) 213, 270, 472, 683
BlsI GCNGC 4 cut(s) 214, 271, 473, 684
Bme1390I CCNGG 3 cut(s) 450, 746, 747
Bme18I GGWCC 1 cut(s) 239
BmeRI GACNNNNNGTC 1 cut(s) 69
BmeT110I CYCGRG 2 cut(s) 745, 929
BmgBI CACGTC 1 cut(s) 120
BmgT120I GGNCC 3 cut(s) 194, 239, 586
BmiI GGNNCC 2 cut(s) 445, 454
BmrFI CCNGG 3 cut(s) 450, 746, 747
BmrI ACTGGG 1 cut(s) 85
BmsI GCATC 3 cut(s) 387, 483, 991
BmuI ACTGGG 1 cut(s) 85
BpmI CTGGAG 1 cut(s) 612
BpuEI CTTGAG 1 cut(s) 116
BpuMI CCSGG 2 cut(s) 746, 747
BsaBI GATNNNNATC 1 cut(s) 603
BsaJI CCNNGG 4 cut(s) 263, 448, 745, 771
BsaWI WCCGGW 1 cut(s) 640
Bsc4I CCNNNNNNNGG 1 cut(s) 97
Bse118I RCCGGY 1 cut(s) 640
Bse1I ACTGG 1 cut(s) 91
Bse21I CCTNAGG 1 cut(s) 385
Bse3DI GCAATG 1 cut(s) 165
Bse8I GATNNNNATC 1 cut(s) 603
BseBI CCWGG 1 cut(s) 450
BseDI CCNNGG 4 cut(s) 263, 448, 745, 771
BseGI GGATG 6 cut(s) 107, 144, 402, 474, 957, 1036
BseJI GATNNNNATC 1 cut(s) 603
BseLI CCNNNNNNNGG 1 cut(s) 97
BseMI GCAATG 1 cut(s) 165
BseMII CTCAG 5 cut(s) 422, 596, 624, 663, 992
BseNI ACTGG 1 cut(s) 91
BseSI GKGCMC 1 cut(s) 448
BseXI GCAGC 4 cut(s) 199, 281, 483, 694
BsgI GTGCAG 1 cut(s) 333
BshFI GGCC 3 cut(s) 196, 588, 898
BshNI GGYRCC 1 cut(s) 443
BshTI ACCGGT 1 cut(s) 640
BsiHKCI CYCGRG 2 cut(s) 745, 929
BsiSI CCGG 3 cut(s) 641, 679, 746
BslI CCNNNNNNNGG 1 cut(s) 97
BsmAI GTCTC 3 cut(s) 69, 498, 506
BsmBI CGTCTC 1 cut(s) 69
BsmI GAATGC 1 cut(s) 258
BsnI GGCC 3 cut(s) 196, 588, 898
BsoBI CYCGRG 2 cut(s) 745, 929
Bsp1286I GDGCHC 1 cut(s) 448
Bsp143I GATC 6 cut(s) 109, 379, 598, 604, 675, 805
Bsp19I CCATGG 1 cut(s) 771
BspACI CCGC 1 cut(s) 98
BspANI GGCC 3 cut(s) 196, 588, 898
BspCNI CTCAG 5 cut(s) 423, 595, 623, 664, 993
BspHI TCATGA 1 cut(s) 1043
BspLI GGNNCC 2 cut(s) 445, 454
BspMI ACCTGC 1 cut(s) 19
BspPI GGATC 3 cut(s) 374, 593, 670
BspQI GCTCTTC 1 cut(s) 898
BspT107I GGYRCC 1 cut(s) 443
BsrDI GCAATG 1 cut(s) 165
BsrFI RCCGGY 1 cut(s) 640
BsrI ACTGG 1 cut(s) 91
BssAI RCCGGY 1 cut(s) 640
BssECI CCNNGG 4 cut(s) 263, 448, 745, 771
BssMI GATC 6 cut(s) 109, 379, 598, 604, 675, 805
BssT1I CCWWGG 1 cut(s) 771
Bst2UI CCWGG 1 cut(s) 450
Bst4CI ACNGT 4 cut(s) 500, 517, 539, 593
Bst6I CTCTTC 1 cut(s) 898
BstC8I GCNNGC 1 cut(s) 648
BstDEI CTNAG 6 cut(s) 385, 431, 582, 610, 672, 1001
BstDSI CCRYGG 2 cut(s) 263, 771
BstF5I GGATG 6 cut(s) 107, 144, 402, 474, 957, 1036
BstKTI GATC 6 cut(s) 112, 382, 601, 607, 678, 808
BstMAI GTCTC 3 cut(s) 69, 498, 506
BstMBI GATC 6 cut(s) 109, 379, 598, 604, 675, 805
BstNI CCWGG 1 cut(s) 450
BstNSI RCATGY 1 cut(s) 732
BstSCI CCNGG 3 cut(s) 448, 744, 745
BstSLI GKGCMC 1 cut(s) 448
BstV1I GCAGC 4 cut(s) 199, 281, 483, 694
BstX2I RGATCY 3 cut(s) 379, 598, 675
BstYI RGATCY 3 cut(s) 379, 598, 675
Bsu36I CCTNAGG 1 cut(s) 385
BsuI GTATCC 1 cut(s) 778
BsuRI GGCC 3 cut(s) 196, 588, 898
BtgI CCRYGG 2 cut(s) 263, 771
BtrI CACGTC 1 cut(s) 120
BtsCI GGATG 6 cut(s) 107, 144, 402, 474, 957, 1036
BtsIMutI CAGTG 2 cut(s) 505, 744
BveI ACCTGC 1 cut(s) 19
Cac8I GCNNGC 1 cut(s) 648
CciI TCATGA 1 cut(s) 1043
Cfr10I RCCGGY 1 cut(s) 640
Cfr13I GGNCC 3 cut(s) 194, 239, 586
Cfr9I CCCGGG 1 cut(s) 745
CseI GACGC 1 cut(s) 218
Csp6I GTAC 1 cut(s) 527
CspAI ACCGGT 1 cut(s) 640
CviAII CATG 8 cut(s) 322, 521, 729, 772, 803, 875, 975, 1044
CviQI GTAC 1 cut(s) 527
DdeI CTNAG 6 cut(s) 385, 431, 582, 610, 672, 1001
DpnI GATC 6 cut(s) 111, 381, 600, 606, 677, 807
DpnII GATC 6 cut(s) 109, 379, 598, 604, 675, 805
DriI GACNNNNNGTC 1 cut(s) 69
EaeI YGGCCR 1 cut(s) 896
Eam1104I CTCTTC 1 cut(s) 898
Eam1105I GACNNNNNGTC 1 cut(s) 69
EarI CTCTTC 1 cut(s) 898
Eco130I CCWWGG 1 cut(s) 771
Eco32I GATATC 1 cut(s) 872
Eco47I GGWCC 1 cut(s) 239
Eco57I CTGAAG 1 cut(s) 396
Eco81I CCTNAGG 1 cut(s) 385
Eco88I CYCGRG 2 cut(s) 745, 929
EcoRII CCWGG 1 cut(s) 448
EcoRV GATATC 1 cut(s) 872
EcoT14I CCWWGG 1 cut(s) 771
EcoT22I ATGCAT 1 cut(s) 45
ErhI CCWWGG 1 cut(s) 771
Esp3I CGTCTC 1 cut(s) 69
FaeI CATG 8 cut(s) 325, 524, 732, 775, 806, 878, 978, 1047
FatI CATG 8 cut(s) 321, 520, 728, 771, 802, 874, 974, 1043
FauI CCCGC 1 cut(s) 91
FbaI TGATCA 1 cut(s) 805
Fnu4HI GCNGC 4 cut(s) 213, 270, 472, 683
FokI GGATG 6 cut(s) 114, 151, 409, 461, 964, 1023
Fsp4HI GCNGC 4 cut(s) 213, 270, 472, 683
FspBI CTAG 1 cut(s) 909
GluI GCNGC 4 cut(s) 213, 270, 472, 683
GsuI CTGGAG 1 cut(s) 612
HaeIII GGCC 3 cut(s) 196, 588, 898
HapII CCGG 3 cut(s) 641, 679, 746
HgaI GACGC 1 cut(s) 218
Hin1II CATG 8 cut(s) 325, 524, 732, 775, 806, 878, 978, 1047
HincII GTYRAC 1 cut(s) 63
HindII GTYRAC 1 cut(s) 63
HinfI GANTC 8 cut(s) 64, 86, 218, 226, 296, 307, 551, 829
HpaII CCGG 3 cut(s) 641, 679, 746
HphI GGTGA 1 cut(s) 191
Hpy166II GTNNAC 2 cut(s) 63, 742
Hpy188I TCNGA 4 cut(s) 109, 114, 432, 613
Hpy8I GTNNAC 2 cut(s) 63, 742
Hpy99I CGWCG 2 cut(s) 124, 212
HpyCH4III ACNGT 4 cut(s) 500, 517, 539, 593
HpyCH4IV ACGT 2 cut(s) 78, 119
HpyCH4V TGCA 5 cut(s) 28, 43, 314, 400, 757
HpyF3I CTNAG 6 cut(s) 385, 431, 582, 610, 672, 1001
HpySE526I ACGT 2 cut(s) 78, 119
Hsp92II CATG 8 cut(s) 325, 524, 732, 775, 806, 878, 978, 1047
Ksp22I TGATCA 1 cut(s) 805
Kzo9I GATC 6 cut(s) 109, 379, 598, 604, 675, 805
LguI GCTCTTC 1 cut(s) 898
Lsp1109I GCAGC 4 cut(s) 199, 281, 483, 694
LweI GCATC 3 cut(s) 387, 483, 991
MaeI CTAG 1 cut(s) 909
MaeII ACGT 2 cut(s) 78, 119
MalI GATC 6 cut(s) 111, 381, 600, 606, 677, 807
MboI GATC 6 cut(s) 109, 379, 598, 604, 675, 805
MboII GAAGA 2 cut(s) 389, 915
MflI RGATCY 3 cut(s) 379, 598, 675
MhlI GDGCHC 1 cut(s) 448
MlsI TGGCCA 1 cut(s) 898
MluCI AATT 3 cut(s) 15, 421, 666
MluNI TGGCCA 1 cut(s) 898
MlyI GAGTC 2 cut(s) 58, 80
MmeI TCCRAC 1 cut(s) 521
MnlI CCTC 7 cut(s) 50, 129, 140, 488, 712, 882, 957
Mox20I TGGCCA 1 cut(s) 898
Mph1103I ATGCAT 1 cut(s) 45
MscI TGGCCA 1 cut(s) 898
MseI TTAA 4 cut(s) 18, 249, 363, 546
MslI CAYNNNNRTG 3 cut(s) 813, 879, 987
Msp20I TGGCCA 1 cut(s) 898
MspA1I CMGCKG 1 cut(s) 685
MspI CCGG 3 cut(s) 641, 679, 746
MspR9I CCNGG 3 cut(s) 450, 746, 747
Mva1269I GAATGC 1 cut(s) 258
MvaI CCWGG 1 cut(s) 450
NciI CCSGG 2 cut(s) 746, 747
NcoI CCATGG 1 cut(s) 771
NdeII GATC 6 cut(s) 109, 379, 598, 604, 675, 805
NlaIII CATG 8 cut(s) 325, 524, 732, 775, 806, 878, 978, 1047
NlaIV GGNNCC 2 cut(s) 445, 454
NsiI ATGCAT 1 cut(s) 45
NspI RCATGY 1 cut(s) 732
PagI TCATGA 1 cut(s) 1043
PciSI GCTCTTC 1 cut(s) 898
PctI GAATGC 1 cut(s) 258
PfeI GAWTC 6 cut(s) 218, 226, 296, 307, 551, 829
PinAI ACCGGT 1 cut(s) 640
PkrI GCNGC 4 cut(s) 214, 271, 473, 684
PleI GAGTC 2 cut(s) 58, 80
PpsI GAGTC 2 cut(s) 58, 80
PshBI ATTAAT 1 cut(s) 18
PsiI TTATAA 1 cut(s) 426
Psp6I CCWGG 1 cut(s) 448
PspGI CCWGG 1 cut(s) 448
PspN4I GGNNCC 2 cut(s) 445, 454
PspPI GGNCC 3 cut(s) 194, 239, 586
PsuI RGATCY 3 cut(s) 379, 598, 675
PvuII CAGCTG 1 cut(s) 685
RsaI GTAC 1 cut(s) 528
RsaNI GTAC 1 cut(s) 527
RseI CAYNNNNRTG 3 cut(s) 813, 879, 987
SapI GCTCTTC 1 cut(s) 898
SaqAI TTAA 4 cut(s) 18, 249, 363, 546
SatI GCNGC 4 cut(s) 213, 270, 472, 683
Sau3AI GATC 6 cut(s) 109, 379, 598, 604, 675, 805
Sau96I GGNCC 3 cut(s) 194, 239, 586
SchI GAGTC 2 cut(s) 58, 80
ScrFI CCNGG 3 cut(s) 450, 746, 747
SduI GDGCHC 1 cut(s) 448
SfaNI GCATC 3 cut(s) 387, 483, 991
SgrAI CRCCGGYG 1 cut(s) 640
SinI GGWCC 1 cut(s) 239
SmaI CCCGGG 1 cut(s) 747
SmiMI CAYNNNNRTG 3 cut(s) 813, 879, 987
SmlI CTYRAG 1 cut(s) 131
SmoI CTYRAG 1 cut(s) 131
Sse9I AATT 3 cut(s) 15, 421, 666
SsiI CCGC 1 cut(s) 98
SspMI CTAG 1 cut(s) 909
StyD4I CCNGG 3 cut(s) 448, 744, 745
StyI CCWWGG 1 cut(s) 771
TaaI ACNGT 4 cut(s) 500, 517, 539, 593
TaiI ACGT 2 cut(s) 81, 122
TaqI TCGA 3 cut(s) 73, 229, 595
TasI AATT 3 cut(s) 15, 421, 666
TfiI GAWTC 6 cut(s) 218, 226, 296, 307, 551, 829
Tru1I TTAA 4 cut(s) 18, 249, 363, 546
Tru9I TTAA 4 cut(s) 18, 249, 363, 546
TscAI CASTG 2 cut(s) 505, 744
TseI GCWGC 4 cut(s) 212, 269, 471, 682
TspDTI ATGAA 5 cut(s) 154, 320, 509, 723, 784
TspGWI ACGGA 2 cut(s) 252, 551
TspMI CCCGGG 1 cut(s) 745
TspRI CASTG 2 cut(s) 505, 744
VpaK11BI GGWCC 1 cut(s) 239
VspI ATTAAT 1 cut(s) 18
XceI RCATGY 1 cut(s) 732
XmaI CCCGGG 1 cut(s) 745
XspI CTAG 1 cut(s) 909
Zsp2I ATGCAT 1 cut(s) 45
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.