RLG00000009290
NAC Family

inactive purple acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0015175
Physical Location & Seq
Forward (+)
50287185 .. 50289551
2367 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000009290

Sequence Viewer

Length: 1287 bp
ATGAGTCGCATCCGTCAGTCTCAGCTTGTCAAAAGCTGCAGCAGCAGCAGCAGAAGCTACCTGAACAAAAGCCCAATGGACTCGTCGTCCTCAGCTGCAAACTGGAACCACTCTTTCCTCTATGTCATCTTCATCTACGCTCTTCTCTACTTCCTCCACACCCAAGTCGTTTATAGATTACTCATAGGCCATGCACCCGTAACCATCAAGAAGACCAGCCCAAATCTCCCTCTTCGCTTCCGCTCCGATGGGACCTTCAAGATTCTCCAGGTGGCTGATATGCATTATGGGAATGGAGGGTTGACTCGGTGTCGAGACGTATTGGACTCCGAGTTTGCGGGATGTTCTGATCGGAACACGTCGCTATTCCTCAAGAGGATGATTGAAGCTGAAAAGCCTGATTTCATTGCCTTTACAGGTGATAATATATTTGGGCCAAGCTCTACCGACGCTGCTGAATCAATGATTCGAGCATTTGGTCCTGCCATTAAATCAGGCATTCCGTGGGCAGCAATTTTGGGAAACCACGACCAAGAATCTACAATGAATCGTGAAGAACTCATGTCCTTTATCTCCCTTATGGACTATTCACTTTCACAAGTTAATCCATTAGCTGAAGATCCCTTAGGAGAATGGATGCAAGACATTGATGGTTTTGGCAATTATAATCTGAGAGTATATGGTGCCCCAGGTTCCTACTTGGCAAATAGCAGCATCCTCAACCTTTTCTTTCTTGACAGTGGAGACAGAGAGACTGTTCATGGAGTACGAACGTACGGTTGGATTAAGGAATCACAACTCCGTTGGCTTCGTGGTGTTTCTCAGGGCCGCAATCGAGATCCAGATCACTCAGACAATGCTTTTTCTCCAGATAAACCACCGGCGCTTGCTTTTTTCCATATCCCAATTCCTGAGATCTGGCAGCTGTGGAATAAAGACATTGTTGGCAAGTTTCAGGAGGCTGTGGCATGTTCATCAGTGAACCCGGGAGTTCTGCAGACCCTTGTATCCATGGGAGATGTGAAAGCTGTGTTCATTGGACATGATCATACCAATGACTTCTGTGGGAATCTTGATGGTGTTTGGTTCTGTTATGGTGGGGGCTTTGGATATCATGGTTATGGAAAGAGGGGGTGGCGAAGAAGAGCTAGGGTCATATTGGCAGAACTCAGGAAAGGGGAGAAAGACTGGATGGGAGTTGAGAGGATTAGGACATGGAAGCGTCTTGATGATGAGAAACTGAGCAAGATTGATGAGCAGTTCTTGTGGGAATACCATCCATCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003993 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009987 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0042578 GO:0044237
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos

Protein Analysis

429

Amino Acids

48.46

Weight (kDa)

6.87

Isoelectric Point (pI)

48.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos PF00149 86 - 351 1.2e-15 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015175)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G57140
fragaria_vesca FvH4_4g07080
malus_domestica MD04G1168800.v1.1
prunus_persica Prupe.1G074200_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0399201
rosa_laevigata RLG00000009290
rosa_multiflora Rmu_co8469863.1_g000001 Rmu_sc0002582.1_g000004 Rmu_sc0024868.1_g000004
rosa_roxburghii Rroxscaffold_5G00344160
rosa_rugosa Rorug04G0015100
rosa_samantha Rh4AG093000 Rh4BG090800 Rh4CG101400 Rh4DG085300
rosa_wichuraiana Rw4G007660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 666
AccB1I GGYRCC 1 cut(s) 683
AccBSI CCGCTC 1 cut(s) 243
AciI CCGC 3 cut(s) 241, 338, 829
AclWI GGATC 2 cut(s) 614, 833
AcuI CTGAAG 1 cut(s) 636
AfaI GTAC 2 cut(s) 768, 776
AflIII ACRYGT 1 cut(s) 357
AgsI TTSAA 2 cut(s) 259, 386
AhdI GACNNNNNGTC 2 cut(s) 85, 309
AjiI CACGTC 1 cut(s) 360
AjnI CCWGG 2 cut(s) 267, 688
AjuI GAANNNNNNNTTGG 2 cut(s) 763, 795
Alw26I GTCTC 4 cut(s) 24, 309, 738, 746
AlwI GGATC 2 cut(s) 614, 833
Ama87I CYCGRG 1 cut(s) 985
AoxI GGCC 3 cut(s) 187, 434, 826
ArsI GACNNNNNNTTYG 2 cut(s) 317, 349
AspLEI GCGC 1 cut(s) 886
AspS9I GGNCC 4 cut(s) 252, 434, 479, 826
AsuC2I CCSGG 2 cut(s) 986, 987
AsuHPI GGTGA 1 cut(s) 431
AvaI CYCGRG 1 cut(s) 985
AvaII GGWCC 2 cut(s) 252, 479
AxyI CCTNAGG 1 cut(s) 625
BaeGI GKGCMC 1 cut(s) 688
BaeI ACNNNNGTAYC 2 cut(s) 990, 1023
BanI GGYRCC 1 cut(s) 683
BbsI GAAGAC 1 cut(s) 218
BbvCI CCTCAGC 1 cut(s) 91
BccI CCATC 6 cut(s) 212, 242, 644, 1070, 1186, 1284
BciT130I CCWGG 2 cut(s) 269, 690
BciVI GTATCC 1 cut(s) 1018
BclI TGATCA 1 cut(s) 1045
BcnI CCSGG 2 cut(s) 986, 987
BcoDI GTCTC 4 cut(s) 24, 309, 738, 746
BfaI CTAG 1 cut(s) 1149
BfmI CTRYAG 2 cut(s) 37, 995
BfoI RGCGCY 1 cut(s) 887
BfuI GTATCC 1 cut(s) 1018
BglII AGATCT 1 cut(s) 915
Bme1390I CCNGG 4 cut(s) 269, 690, 986, 987
Bme18I GGWCC 2 cut(s) 252, 479
BmeRI GACNNNNNGTC 2 cut(s) 85, 309
BmeT110I CYCGRG 1 cut(s) 985
BmgBI CACGTC 1 cut(s) 360
BmgT120I GGNCC 4 cut(s) 252, 434, 479, 826
BmiI GGNNCC 4 cut(s) 107, 253, 685, 694
BmrFI CCNGG 4 cut(s) 269, 690, 986, 987
BmsI GCATC 3 cut(s) 18, 627, 723
BpiI GAAGAC 1 cut(s) 218
BpmI CTGGAG 2 cut(s) 251, 852
Bpu10I CCTNAGC 1 cut(s) 91
BpuEI CTTGAG 1 cut(s) 356
BpuMI CCSGG 2 cut(s) 986, 987
BsaBI GATNNNNATC 1 cut(s) 843
BsaJI CCNNGG 4 cut(s) 503, 688, 985, 1011
Bse118I RCCGGY 1 cut(s) 880
Bse1I ACTGG 2 cut(s) 107, 1193
Bse21I CCTNAGG 1 cut(s) 625
Bse3DI GCAATG 1 cut(s) 405
Bse8I GATNNNNATC 1 cut(s) 843
BseBI CCWGG 2 cut(s) 269, 690
BseDI CCNNGG 4 cut(s) 503, 688, 985, 1011
BseGI GGATG 7 cut(s) 9, 347, 384, 642, 714, 1197, 1276
BseJI GATNNNNATC 1 cut(s) 843
BseMI GCAATG 1 cut(s) 405
BseMII CTCAG 8 cut(s) 35, 105, 662, 836, 864, 903, 1183, 1232
BseNI ACTGG 2 cut(s) 107, 1193
BseSI GKGCMC 1 cut(s) 688
BshFI GGCC 3 cut(s) 189, 436, 828
BshNI GGYRCC 1 cut(s) 683
BsiHKCI CYCGRG 1 cut(s) 985
BsiSI CCGG 2 cut(s) 881, 986
BsiWI CGTACG 1 cut(s) 774
BslFI GGGAC 1 cut(s) 265
BsmAI GTCTC 4 cut(s) 24, 309, 738, 746
BsmBI CGTCTC 1 cut(s) 309
BsmFI GGGAC 1 cut(s) 265
BsmI GAATGC 1 cut(s) 498
BsnI GGCC 3 cut(s) 189, 436, 828
BsoBI CYCGRG 1 cut(s) 985
Bsp1286I GDGCHC 1 cut(s) 688
Bsp143I GATC 6 cut(s) 349, 619, 838, 844, 915, 1045
Bsp19I CCATGG 1 cut(s) 1011
BspACI CCGC 3 cut(s) 241, 338, 829
BspANI GGCC 3 cut(s) 189, 436, 828
BspCNI CTCAG 8 cut(s) 34, 104, 663, 835, 863, 904, 1182, 1233
BspHI TCATGA 1 cut(s) 1283
BspLI GGNNCC 4 cut(s) 107, 253, 685, 694
BspMAI CTGCAG 2 cut(s) 41, 999
BspPI GGATC 2 cut(s) 614, 833
BspQI GCTCTTC 2 cut(s) 147, 1138
BspT107I GGYRCC 1 cut(s) 683
BsrBI CCGCTC 1 cut(s) 243
BsrDI GCAATG 1 cut(s) 405
BsrFI RCCGGY 1 cut(s) 880
BsrI ACTGG 2 cut(s) 107, 1193
BssAI RCCGGY 1 cut(s) 880
BssECI CCNNGG 4 cut(s) 503, 688, 985, 1011
BssMI GATC 6 cut(s) 349, 619, 838, 844, 915, 1045
BssT1I CCWWGG 1 cut(s) 1011
Bst2UI CCWGG 2 cut(s) 269, 690
Bst4CI ACNGT 3 cut(s) 740, 757, 779
Bst6I CTCTTC 3 cut(s) 147, 237, 1138
BstC8I GCNNGC 1 cut(s) 888
BstDEI CTNAG 9 cut(s) 21, 91, 625, 671, 822, 850, 912, 1169, 1241
BstDSI CCRYGG 2 cut(s) 503, 1011
BstF5I GGATG 7 cut(s) 9, 347, 384, 642, 714, 1197, 1276
BstH2I RGCGCY 1 cut(s) 887
BstHHI GCGC 1 cut(s) 886
BstKTI GATC 6 cut(s) 352, 622, 841, 847, 918, 1048
BstMAI GTCTC 4 cut(s) 24, 309, 738, 746
BstMBI GATC 6 cut(s) 349, 619, 838, 844, 915, 1045
BstMWI GCNNNNNNNGC 4 cut(s) 42, 45, 48, 54
BstNI CCWGG 2 cut(s) 269, 690
BstNSI RCATGY 1 cut(s) 972
BstSCI CCNGG 4 cut(s) 267, 688, 984, 985
BstSFI CTRYAG 2 cut(s) 37, 995
BstSLI GKGCMC 1 cut(s) 688
BstV2I GAAGAC 1 cut(s) 218
BstX2I RGATCY 3 cut(s) 619, 838, 915
BstYI RGATCY 3 cut(s) 619, 838, 915
Bsu36I CCTNAGG 1 cut(s) 625
BsuI GTATCC 1 cut(s) 1018
BsuRI GGCC 3 cut(s) 189, 436, 828
BtgI CCRYGG 2 cut(s) 503, 1011
BtrI CACGTC 1 cut(s) 360
BtsCI GGATG 7 cut(s) 9, 347, 384, 642, 714, 1197, 1276
BtsIMutI CAGTG 2 cut(s) 745, 984
Cac8I GCNNGC 1 cut(s) 888
CciI TCATGA 1 cut(s) 1283
CfoI GCGC 1 cut(s) 886
Cfr10I RCCGGY 1 cut(s) 880
Cfr13I GGNCC 4 cut(s) 252, 434, 479, 826
Cfr9I CCCGGG 1 cut(s) 985
CseI GACGC 2 cut(s) 458, 1211
Csp6I GTAC 2 cut(s) 767, 775
CviAII CATG 9 cut(s) 191, 562, 761, 969, 1012, 1043, 1115, 1215, 1284
CviQI GTAC 2 cut(s) 767, 775
DdeI CTNAG 9 cut(s) 21, 91, 625, 671, 822, 850, 912, 1169, 1241
DpnI GATC 6 cut(s) 351, 621, 840, 846, 917, 1047
DpnII GATC 6 cut(s) 349, 619, 838, 844, 915, 1045
DriI GACNNNNNGTC 2 cut(s) 85, 309
Eam1104I CTCTTC 3 cut(s) 147, 237, 1138
Eam1105I GACNNNNNGTC 2 cut(s) 85, 309
EarI CTCTTC 3 cut(s) 147, 237, 1138
Eco130I CCWWGG 1 cut(s) 1011
Eco32I GATATC 1 cut(s) 1112
Eco47I GGWCC 2 cut(s) 252, 479
Eco57I CTGAAG 1 cut(s) 636
Eco81I CCTNAGG 1 cut(s) 625
Eco88I CYCGRG 1 cut(s) 985
EcoO109I RGGNCCY 1 cut(s) 252
EcoRII CCWGG 2 cut(s) 267, 688
EcoRV GATATC 1 cut(s) 1112
EcoT14I CCWWGG 1 cut(s) 1011
EcoT22I ATGCAT 1 cut(s) 285
ErhI CCWWGG 1 cut(s) 1011
Esp3I CGTCTC 1 cut(s) 309
FaeI CATG 9 cut(s) 194, 565, 764, 972, 1015, 1046, 1118, 1218, 1287
FaqI GGGAC 1 cut(s) 265
FatI CATG 9 cut(s) 190, 561, 760, 968, 1011, 1042, 1114, 1214, 1283
FauI CCCGC 1 cut(s) 331
FbaI TGATCA 1 cut(s) 1045
FokI GGATG 6 cut(s) 354, 391, 649, 701, 1204, 1263
FspBI CTAG 1 cut(s) 1149
GlaI GCGC 1 cut(s) 885
GsuI CTGGAG 2 cut(s) 251, 852
HaeII RGCGCY 1 cut(s) 887
HaeIII GGCC 3 cut(s) 189, 436, 828
HapII CCGG 2 cut(s) 881, 986
HgaI GACGC 2 cut(s) 458, 1211
HhaI GCGC 1 cut(s) 886
Hin1II CATG 9 cut(s) 194, 565, 764, 972, 1015, 1046, 1118, 1218, 1287
Hin6I GCGC 1 cut(s) 884
HinP1I GCGC 1 cut(s) 884
HincII GTYRAC 1 cut(s) 303
HindII GTYRAC 1 cut(s) 303
HpaII CCGG 2 cut(s) 881, 986
HphI GGTGA 1 cut(s) 431
Hpy166II GTNNAC 2 cut(s) 303, 982
Hpy188I TCNGA 6 cut(s) 247, 331, 349, 354, 672, 853
Hpy8I GTNNAC 2 cut(s) 303, 982
Hpy99I CGWCG 3 cut(s) 88, 364, 452
HpyAV CCTTC 1 cut(s) 265
HpyCH4III ACNGT 3 cut(s) 740, 757, 779
HpyCH4IV ACGT 3 cut(s) 318, 359, 773
HpyCH4V TGCA 6 cut(s) 39, 98, 194, 283, 640, 997
HpyF10VI GCNNNNNNNGC 4 cut(s) 42, 45, 48, 54
HpyF3I CTNAG 9 cut(s) 21, 91, 625, 671, 822, 850, 912, 1169, 1241
HpySE526I ACGT 3 cut(s) 318, 359, 773
Hsp92II CATG 9 cut(s) 194, 565, 764, 972, 1015, 1046, 1118, 1218, 1287
HspAI GCGC 1 cut(s) 884
Ksp22I TGATCA 1 cut(s) 1045
Kzo9I GATC 6 cut(s) 349, 619, 838, 844, 915, 1045
LguI GCTCTTC 2 cut(s) 147, 1138
LmnI GCTCC 1 cut(s) 248
LweI GCATC 3 cut(s) 18, 627, 723
MaeI CTAG 1 cut(s) 1149
MaeII ACGT 3 cut(s) 318, 359, 773
MaeIII GTNAC 1 cut(s) 199
MalI GATC 6 cut(s) 351, 621, 840, 846, 917, 1047
MbiI CCGCTC 1 cut(s) 243
MboI GATC 6 cut(s) 349, 619, 838, 844, 915, 1045
MboII GAAGA 8 cut(s) 121, 134, 223, 224, 566, 629, 1152, 1155
MflI RGATCY 3 cut(s) 619, 838, 915
MhlI GDGCHC 1 cut(s) 688
MluCI AATT 3 cut(s) 513, 661, 906
MlyI GAGTC 4 cut(s) 13, 74, 298, 320
MmeI TCCRAC 1 cut(s) 761
Mph1103I ATGCAT 1 cut(s) 285
MseI TTAA 3 cut(s) 489, 603, 786
MslI CAYNNNNRTG 2 cut(s) 1053, 1119
MspA1I CMGCKG 2 cut(s) 95, 925
MspI CCGG 2 cut(s) 881, 986
MspR9I CCNGG 4 cut(s) 269, 690, 986, 987
Mva1269I GAATGC 1 cut(s) 498
MvaI CCWGG 2 cut(s) 269, 690
MwoI GCNNNNNNNGC 4 cut(s) 42, 45, 48, 54
NciI CCSGG 2 cut(s) 986, 987
NcoI CCATGG 1 cut(s) 1011
NdeII GATC 6 cut(s) 349, 619, 838, 844, 915, 1045
NlaIII CATG 9 cut(s) 194, 565, 764, 972, 1015, 1046, 1118, 1218, 1287
NlaIV GGNNCC 4 cut(s) 107, 253, 685, 694
NsiI ATGCAT 1 cut(s) 285
NspI RCATGY 1 cut(s) 972
PagI TCATGA 1 cut(s) 1283
PciSI GCTCTTC 2 cut(s) 147, 1138
PctI GAATGC 1 cut(s) 498
PfeI GAWTC 7 cut(s) 262, 458, 466, 536, 547, 791, 1069
Pfl23II CGTACG 1 cut(s) 774
PleI GAGTC 4 cut(s) 12, 74, 298, 320
PpsI GAGTC 4 cut(s) 12, 74, 298, 320
PpuMI RGGWCCY 1 cut(s) 252
PsiI TTATAA 1 cut(s) 666
Psp5II RGGWCCY 1 cut(s) 252
Psp6I CCWGG 2 cut(s) 267, 688
PspGI CCWGG 2 cut(s) 267, 688
PspLI CGTACG 1 cut(s) 774
PspN4I GGNNCC 4 cut(s) 107, 253, 685, 694
PspPI GGNCC 4 cut(s) 252, 434, 479, 826
PspPPI RGGWCCY 1 cut(s) 252
PstI CTGCAG 2 cut(s) 41, 999
PsuI RGATCY 3 cut(s) 619, 838, 915
PvuII CAGCTG 2 cut(s) 95, 925
RsaI GTAC 2 cut(s) 768, 776
RsaNI GTAC 2 cut(s) 767, 775
RseI CAYNNNNRTG 2 cut(s) 1053, 1119
SapI GCTCTTC 2 cut(s) 147, 1138
SaqAI TTAA 3 cut(s) 489, 603, 786
Sau3AI GATC 6 cut(s) 349, 619, 838, 844, 915, 1045
Sau96I GGNCC 4 cut(s) 252, 434, 479, 826
SchI GAGTC 4 cut(s) 13, 74, 298, 320
ScrFI CCNGG 4 cut(s) 269, 690, 986, 987
SduI GDGCHC 1 cut(s) 688
SfaNI GCATC 3 cut(s) 18, 627, 723
SfcI CTRYAG 2 cut(s) 37, 995
SgrAI CRCCGGYG 1 cut(s) 880
SinI GGWCC 2 cut(s) 252, 479
SmaI CCCGGG 1 cut(s) 987
SmiMI CAYNNNNRTG 2 cut(s) 1053, 1119
SmlI CTYRAG 1 cut(s) 371
SmoI CTYRAG 1 cut(s) 371
Sse9I AATT 3 cut(s) 513, 661, 906
SsiI CCGC 3 cut(s) 241, 338, 829
SspMI CTAG 1 cut(s) 1149
StyD4I CCNGG 4 cut(s) 267, 688, 984, 985
StyI CCWWGG 1 cut(s) 1011
TaaI ACNGT 3 cut(s) 740, 757, 779
TaiI ACGT 3 cut(s) 321, 362, 776
TaqI TCGA 3 cut(s) 313, 469, 835
TasI AATT 3 cut(s) 513, 661, 906
TauI GCSGC 1 cut(s) 831
TfiI GAWTC 7 cut(s) 262, 458, 466, 536, 547, 791, 1069
Tru1I TTAA 3 cut(s) 489, 603, 786
Tru9I TTAA 3 cut(s) 489, 603, 786
TscAI CASTG 2 cut(s) 745, 984
TspDTI ATGAA 6 cut(s) 121, 394, 560, 749, 963, 1024
TspGWI ACGGA 2 cut(s) 492, 791
TspMI CCCGGG 1 cut(s) 985
TspRI CASTG 2 cut(s) 745, 984
VpaK11BI GGWCC 2 cut(s) 252, 479
XceI RCATGY 1 cut(s) 972
XmaI CCCGGG 1 cut(s) 985
XspI CTAG 1 cut(s) 1149
Zsp2I ATGCAT 1 cut(s) 285
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.