Rh4BG090800
NAC Family

inactive purple acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0015175
Physical Location & Seq
Reverse (-)
15539566 .. 15541870
2305 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG090800.1

Sequence Viewer

Length: 1212 bp
ATGGACTCGTCGTCCTCAGCTGCAAACTGGAACCACTCTTTCCTCTATCTCATCTTCATCTACGCTCTTCTCTACTTCCTCCACACCCAAATCGCTCACAGATTACTCATAGGCCATGCACCCGTAACCATCAAGAAGACCAGCCCAAATCTCCCTCTTCGCTTCCGCTCCGATGGCACCTTCAAGATTCTCCAGGTGGCTGATATGCATTATGGGAATGGAGGGTTGACTCGGTGTCGAGACGTATTGGACTCCCAGTTTGCGGGATGTTCTGATCGGAACACGTCGCTGTTCCTCAAGAGGATGATTGAAGCTGAAAAGCCTGATTTCATTGCTTTTACAGGTGATAATATATTTGGGCCAAGCTCTACCGACGCTGCTGAATCAATGATTCGAGCATTTGGTCCTGCCATTAAATCAGGCATTCCGTGGGCAGCAGTTTTGGGAAACCACGACCAAGAATCTACAATGAATCGTGAAGAACTCATGTCCTTTATCTCCCTTATGGACTATTCACTTTCACAAGTTAATCCATTAGCTGACGATCCCTTAGGAGAATGGATGCAAGACATTGATGGTTTTGGCAATTATAATCTGAGAGTATATGGTGCCTCAGGTTCCTACTTGGCAAATAGCAGCATCCTCAACCTTTTCTTTCTTGACAGTGGAGACAGAGAGACTGTTCAGGGAGTACGAACTTACGGTTGGATTAAGGAATCACAACTCCGTTGGCTTCGTGGTGTTTCTCAGGGCCACAGTCGAGATCCAGATCACTCAGACAATGCTTTTTCTCCAGATAAACCACCGGCACTTGCTTTTTTCCATATCCCAATTCCTGAGATCCGGCAGCTGTGGAATAAAGACATTGTTGGCAAGTTTCAGGAGGCTGTGGCATGTTCATCAGTGAACCCGGGAGTTTTGCAGACCCTTGTATCCATGGGAGATGTGAAAGCTGTGTTCATTGGACATGATCATACCAATGACTTCTGTGGGAATCTTGATGGTGTTTGGTTCTGTTATGGTGGGGGCTTTGGATATCATGGTTATGGAAAGAGGGGGTGTCCAAGAAGAGCTAGGGTCATATTGGCAGAACTGGGGAAAGGGGAGAAAGGCTGGATGGGAGTTGAGAGGATTAGGACATGGAAGCGTCTTGATGATGAGAAACTGAGCAAGATTGATGAGCAGTTCTTGTGGGAATACCATCCATCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003993 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009987 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0042578 GO:0044237
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos

Protein Analysis

403

Amino Acids

45.2

Weight (kDa)

6.11

Isoelectric Point (pI)

40.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos PF00149 61 - 326 4.5e-15 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015175)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G57140
fragaria_vesca FvH4_4g07080
malus_domestica MD04G1168800.v1.1
prunus_persica Prupe.1G074200_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0399201
rosa_laevigata RLG00000009290
rosa_multiflora Rmu_co8469863.1_g000001 Rmu_sc0002582.1_g000004 Rmu_sc0024868.1_g000004
rosa_roxburghii Rroxscaffold_5G00344160
rosa_rugosa Rorug04G0015100
rosa_samantha Rh4AG093000 Rh4BG090800 Rh4CG101400 Rh4DG085300
rosa_wichuraiana Rw4G007660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 591
AccB1I GGYRCC 2 cut(s) 176, 608
AccBSI CCGCTC 1 cut(s) 168
AciI CCGC 2 cut(s) 166, 263
AclWI GGATC 3 cut(s) 539, 758, 835
AfaI GTAC 1 cut(s) 693
AfiI CCNNNNNNNGG 1 cut(s) 262
AflIII ACRYGT 1 cut(s) 282
AgsI TTSAA 2 cut(s) 184, 311
AhdI GACNNNNNGTC 2 cut(s) 10, 234
AjiI CACGTC 1 cut(s) 285
AjnI CCWGG 1 cut(s) 192
AjuI GAANNNNNNNTTGG 2 cut(s) 688, 720
AluBI AGCT 7 cut(s) 20, 314, 366, 539, 850, 953, 1073
AluI AGCT 7 cut(s) 20, 314, 366, 539, 850, 953, 1073
Alw26I GTCTC 3 cut(s) 234, 663, 671
AlwI GGATC 3 cut(s) 539, 758, 835
Ama87I CYCGRG 1 cut(s) 910
AoxI GGCC 3 cut(s) 112, 359, 751
ApeKI GCWGC 5 cut(s) 20, 377, 434, 636, 847
ArsI GACNNNNNNTTYG 2 cut(s) 242, 274
AspS9I GGNCC 3 cut(s) 359, 404, 751
AsuC2I CCSGG 2 cut(s) 911, 912
AsuHPI GGTGA 1 cut(s) 356
AvaI CYCGRG 1 cut(s) 910
AvaII GGWCC 1 cut(s) 404
AxyI CCTNAGG 2 cut(s) 550, 613
BaeI ACNNNNGTAYC 2 cut(s) 915, 948
BanI GGYRCC 2 cut(s) 176, 608
BbsI GAAGAC 1 cut(s) 143
BbvCI CCTCAGC 1 cut(s) 16
BbvI GCAGC 5 cut(s) 7, 364, 446, 648, 859
BccI CCATC 6 cut(s) 137, 167, 569, 995, 1111, 1209
BciT130I CCWGG 1 cut(s) 194
BciVI GTATCC 1 cut(s) 943
BclI TGATCA 1 cut(s) 970
BcnI CCSGG 2 cut(s) 911, 912
BcoDI GTCTC 3 cut(s) 234, 663, 671
BfaI CTAG 1 cut(s) 1074
BfuI GTATCC 1 cut(s) 943
BisI GCNGC 5 cut(s) 21, 378, 435, 637, 848
BlsI GCNGC 5 cut(s) 22, 379, 436, 638, 849
Bme1390I CCNGG 3 cut(s) 194, 911, 912
Bme18I GGWCC 1 cut(s) 404
BmeRI GACNNNNNGTC 2 cut(s) 10, 234
BmeT110I CYCGRG 1 cut(s) 910
BmgBI CACGTC 1 cut(s) 285
BmgT120I GGNCC 3 cut(s) 359, 404, 751
BmiI GGNNCC 4 cut(s) 32, 178, 610, 619
BmrFI CCNGG 3 cut(s) 194, 911, 912
BmrI ACTGGG 2 cut(s) 250, 1103
BmsI GCATC 2 cut(s) 552, 648
BmuI ACTGGG 2 cut(s) 250, 1103
BpiI GAAGAC 1 cut(s) 143
BpmI CTGGAG 2 cut(s) 176, 777
Bpu10I CCTNAGC 1 cut(s) 16
BpuEI CTTGAG 1 cut(s) 281
BpuMI CCSGG 2 cut(s) 911, 912
BsaBI GATNNNNATC 1 cut(s) 768
BsaJI CCNNGG 3 cut(s) 428, 910, 936
Bsc4I CCNNNNNNNGG 1 cut(s) 262
Bse118I RCCGGY 1 cut(s) 805
Bse1I ACTGG 3 cut(s) 32, 256, 1098
Bse21I CCTNAGG 2 cut(s) 550, 613
Bse3DI GCAATG 1 cut(s) 330
Bse8I GATNNNNATC 1 cut(s) 768
BseBI CCWGG 1 cut(s) 194
BseDI CCNNGG 3 cut(s) 428, 910, 936
BseGI GGATG 6 cut(s) 272, 309, 567, 639, 1122, 1201
BseJI GATNNNNATC 1 cut(s) 768
BseLI CCNNNNNNNGG 1 cut(s) 262
BseMI GCAATG 1 cut(s) 330
BseMII CTCAG 7 cut(s) 30, 587, 627, 761, 789, 828, 1157
BseNI ACTGG 3 cut(s) 32, 256, 1098
BseXI GCAGC 5 cut(s) 7, 364, 446, 648, 859
BshFI GGCC 3 cut(s) 114, 361, 753
BshNI GGYRCC 2 cut(s) 176, 608
BsiHKCI CYCGRG 1 cut(s) 910
BsiSI CCGG 3 cut(s) 806, 844, 911
BslI CCNNNNNNNGG 1 cut(s) 262
BsmAI GTCTC 3 cut(s) 234, 663, 671
BsmBI CGTCTC 1 cut(s) 234
BsmI GAATGC 1 cut(s) 423
BsnI GGCC 3 cut(s) 114, 361, 753
BsoBI CYCGRG 1 cut(s) 910
Bsp143I GATC 6 cut(s) 274, 544, 763, 769, 840, 970
Bsp19I CCATGG 1 cut(s) 936
BspACI CCGC 2 cut(s) 166, 263
BspANI GGCC 3 cut(s) 114, 361, 753
BspCNI CTCAG 7 cut(s) 29, 588, 626, 760, 788, 829, 1158
BspHI TCATGA 1 cut(s) 1208
BspLI GGNNCC 4 cut(s) 32, 178, 610, 619
BspPI GGATC 3 cut(s) 539, 758, 835
BspQI GCTCTTC 2 cut(s) 72, 1063
BspT107I GGYRCC 2 cut(s) 176, 608
BsrBI CCGCTC 1 cut(s) 168
BsrDI GCAATG 1 cut(s) 330
BsrFI RCCGGY 1 cut(s) 805
BsrI ACTGG 3 cut(s) 32, 256, 1098
BssAI RCCGGY 1 cut(s) 805
BssECI CCNNGG 3 cut(s) 428, 910, 936
BssMI GATC 6 cut(s) 274, 544, 763, 769, 840, 970
BssT1I CCWWGG 1 cut(s) 936
Bst2UI CCWGG 1 cut(s) 194
Bst4CI ACNGT 4 cut(s) 665, 682, 704, 758
Bst6I CTCTTC 3 cut(s) 72, 162, 1063
BstDEI CTNAG 8 cut(s) 16, 550, 596, 613, 747, 775, 837, 1166
BstDSI CCRYGG 2 cut(s) 428, 936
BstF5I GGATG 6 cut(s) 272, 309, 567, 639, 1122, 1201
BstKTI GATC 6 cut(s) 277, 547, 766, 772, 843, 973
BstMAI GTCTC 3 cut(s) 234, 663, 671
BstMBI GATC 6 cut(s) 274, 544, 763, 769, 840, 970
BstMWI GCNNNNNNNGC 1 cut(s) 174
BstNI CCWGG 1 cut(s) 194
BstNSI RCATGY 1 cut(s) 897
BstSCI CCNGG 3 cut(s) 192, 909, 910
BstV1I GCAGC 5 cut(s) 7, 364, 446, 648, 859
BstV2I GAAGAC 1 cut(s) 143
BstX2I RGATCY 2 cut(s) 763, 840
BstYI RGATCY 2 cut(s) 763, 840
Bsu36I CCTNAGG 2 cut(s) 550, 613
BsuI GTATCC 1 cut(s) 943
BsuRI GGCC 3 cut(s) 114, 361, 753
BtgI CCRYGG 2 cut(s) 428, 936
BtrI CACGTC 1 cut(s) 285
BtsCI GGATG 6 cut(s) 272, 309, 567, 639, 1122, 1201
BtsIMutI CAGTG 2 cut(s) 670, 909
CciI TCATGA 1 cut(s) 1208
Cfr10I RCCGGY 1 cut(s) 805
Cfr13I GGNCC 3 cut(s) 359, 404, 751
Cfr9I CCCGGG 1 cut(s) 910
CseI GACGC 2 cut(s) 383, 1136
Csp6I GTAC 1 cut(s) 692
CviAII CATG 8 cut(s) 116, 487, 894, 937, 968, 1040, 1140, 1209
CviQI GTAC 1 cut(s) 692
DdeI CTNAG 8 cut(s) 16, 550, 596, 613, 747, 775, 837, 1166
DpnI GATC 6 cut(s) 276, 546, 765, 771, 842, 972
DpnII GATC 6 cut(s) 274, 544, 763, 769, 840, 970
DriI GACNNNNNGTC 2 cut(s) 10, 234
Eam1104I CTCTTC 3 cut(s) 72, 162, 1063
Eam1105I GACNNNNNGTC 2 cut(s) 10, 234
EarI CTCTTC 3 cut(s) 72, 162, 1063
Eco130I CCWWGG 1 cut(s) 936
Eco32I GATATC 1 cut(s) 1037
Eco47I GGWCC 1 cut(s) 404
Eco81I CCTNAGG 2 cut(s) 550, 613
Eco88I CYCGRG 1 cut(s) 910
EcoRII CCWGG 1 cut(s) 192
EcoRV GATATC 1 cut(s) 1037
EcoT14I CCWWGG 1 cut(s) 936
EcoT22I ATGCAT 1 cut(s) 210
ErhI CCWWGG 1 cut(s) 936
Esp3I CGTCTC 1 cut(s) 234
FaeI CATG 8 cut(s) 119, 490, 897, 940, 971, 1043, 1143, 1212
FatI CATG 8 cut(s) 115, 486, 893, 936, 967, 1039, 1139, 1208
FauI CCCGC 1 cut(s) 256
FbaI TGATCA 1 cut(s) 970
Fnu4HI GCNGC 5 cut(s) 21, 378, 435, 637, 848
FokI GGATG 6 cut(s) 279, 316, 574, 626, 1129, 1188
Fsp4HI GCNGC 5 cut(s) 21, 378, 435, 637, 848
FspBI CTAG 1 cut(s) 1074
GluI GCNGC 5 cut(s) 21, 378, 435, 637, 848
GsuI CTGGAG 2 cut(s) 176, 777
HaeIII GGCC 3 cut(s) 114, 361, 753
HapII CCGG 3 cut(s) 806, 844, 911
HgaI GACGC 2 cut(s) 383, 1136
Hin1II CATG 8 cut(s) 119, 490, 897, 940, 971, 1043, 1143, 1212
HincII GTYRAC 1 cut(s) 228
HindII GTYRAC 1 cut(s) 228
HpaII CCGG 3 cut(s) 806, 844, 911
HphI GGTGA 1 cut(s) 356
Hpy166II GTNNAC 2 cut(s) 228, 907
Hpy188I TCNGA 5 cut(s) 172, 274, 279, 597, 778
Hpy8I GTNNAC 2 cut(s) 228, 907
Hpy99I CGWCG 3 cut(s) 13, 289, 377
HpyAV CCTTC 1 cut(s) 190
HpyCH4III ACNGT 4 cut(s) 665, 682, 704, 758
HpyCH4IV ACGT 2 cut(s) 243, 284
HpyCH4V TGCA 5 cut(s) 23, 119, 208, 565, 922
HpyF10VI GCNNNNNNNGC 1 cut(s) 174
HpyF3I CTNAG 8 cut(s) 16, 550, 596, 613, 747, 775, 837, 1166
HpySE526I ACGT 2 cut(s) 243, 284
Hsp92II CATG 8 cut(s) 119, 490, 897, 940, 971, 1043, 1143, 1212
Ksp22I TGATCA 1 cut(s) 970
Kzo9I GATC 6 cut(s) 274, 544, 763, 769, 840, 970
LguI GCTCTTC 2 cut(s) 72, 1063
LmnI GCTCC 1 cut(s) 173
Lsp1109I GCAGC 5 cut(s) 7, 364, 446, 648, 859
LweI GCATC 2 cut(s) 552, 648
MaeI CTAG 1 cut(s) 1074
MaeII ACGT 2 cut(s) 243, 284
MaeIII GTNAC 1 cut(s) 124
MalI GATC 6 cut(s) 276, 546, 765, 771, 842, 972
MbiI CCGCTC 1 cut(s) 168
MboI GATC 6 cut(s) 274, 544, 763, 769, 840, 970
MboII GAAGA 6 cut(s) 46, 59, 148, 149, 491, 1080
MflI RGATCY 2 cut(s) 763, 840
MluCI AATT 2 cut(s) 586, 831
MlyI GAGTC 2 cut(s) 223, 245
MmeI TCCRAC 1 cut(s) 686
Mph1103I ATGCAT 1 cut(s) 210
MseI TTAA 3 cut(s) 414, 528, 711
MslI CAYNNNNRTG 2 cut(s) 978, 1044
MspA1I CMGCKG 2 cut(s) 20, 850
MspI CCGG 3 cut(s) 806, 844, 911
MspR9I CCNGG 3 cut(s) 194, 911, 912
Mva1269I GAATGC 1 cut(s) 423
MvaI CCWGG 1 cut(s) 194
MwoI GCNNNNNNNGC 1 cut(s) 174
NciI CCSGG 2 cut(s) 911, 912
NcoI CCATGG 1 cut(s) 936
NdeII GATC 6 cut(s) 274, 544, 763, 769, 840, 970
NlaIII CATG 8 cut(s) 119, 490, 897, 940, 971, 1043, 1143, 1212
NlaIV GGNNCC 4 cut(s) 32, 178, 610, 619
NsiI ATGCAT 1 cut(s) 210
NspI RCATGY 1 cut(s) 897
PagI TCATGA 1 cut(s) 1208
PciSI GCTCTTC 2 cut(s) 72, 1063
PctI GAATGC 1 cut(s) 423
PfeI GAWTC 7 cut(s) 187, 383, 391, 461, 472, 716, 994
PkrI GCNGC 5 cut(s) 22, 379, 436, 638, 849
PleI GAGTC 2 cut(s) 223, 245
PpsI GAGTC 2 cut(s) 223, 245
PsiI TTATAA 1 cut(s) 591
Psp6I CCWGG 1 cut(s) 192
PspGI CCWGG 1 cut(s) 192
PspN4I GGNNCC 4 cut(s) 32, 178, 610, 619
PspPI GGNCC 3 cut(s) 359, 404, 751
PsuI RGATCY 2 cut(s) 763, 840
PvuII CAGCTG 2 cut(s) 20, 850
RsaI GTAC 1 cut(s) 693
RsaNI GTAC 1 cut(s) 692
RseI CAYNNNNRTG 2 cut(s) 978, 1044
SapI GCTCTTC 2 cut(s) 72, 1063
SaqAI TTAA 3 cut(s) 414, 528, 711
SatI GCNGC 5 cut(s) 21, 378, 435, 637, 848
Sau3AI GATC 6 cut(s) 274, 544, 763, 769, 840, 970
Sau96I GGNCC 3 cut(s) 359, 404, 751
SchI GAGTC 2 cut(s) 223, 245
ScrFI CCNGG 3 cut(s) 194, 911, 912
SfaNI GCATC 2 cut(s) 552, 648
SinI GGWCC 1 cut(s) 404
SmaI CCCGGG 1 cut(s) 912
SmiMI CAYNNNNRTG 2 cut(s) 978, 1044
SmlI CTYRAG 1 cut(s) 296
SmoI CTYRAG 1 cut(s) 296
Sse9I AATT 2 cut(s) 586, 831
SsiI CCGC 2 cut(s) 166, 263
SspMI CTAG 1 cut(s) 1074
StyD4I CCNGG 3 cut(s) 192, 909, 910
StyI CCWWGG 1 cut(s) 936
TaaI ACNGT 4 cut(s) 665, 682, 704, 758
TaiI ACGT 2 cut(s) 246, 287
TaqI TCGA 3 cut(s) 238, 394, 760
TasI AATT 2 cut(s) 586, 831
TfiI GAWTC 7 cut(s) 187, 383, 391, 461, 472, 716, 994
Tru1I TTAA 3 cut(s) 414, 528, 711
Tru9I TTAA 3 cut(s) 414, 528, 711
TscAI CASTG 2 cut(s) 670, 909
TseI GCWGC 5 cut(s) 20, 377, 434, 636, 847
TspDTI ATGAA 5 cut(s) 46, 319, 485, 888, 949
TspGWI ACGGA 2 cut(s) 417, 716
TspMI CCCGGG 1 cut(s) 910
TspRI CASTG 2 cut(s) 670, 909
VpaK11BI GGWCC 1 cut(s) 404
XceI RCATGY 1 cut(s) 897
XmaI CCCGGG 1 cut(s) 910
XspI CTAG 1 cut(s) 1074
Zsp2I ATGCAT 1 cut(s) 210
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.