Rh4AG093000
NAC Family

inactive purple acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0015175
Physical Location & Seq
Reverse (-)
19711912 .. 19714224
2313 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG093000.1

Sequence Viewer

Length: 1212 bp
ATGGACTCGTCGTCCTCAGCTGCAAACTGGAACCACTCTTTCCTCTATCTCATCTTCATCTACGCTCTTCTCTACTTCCTCCACACCCAAATCGCTCACAGATTACTCATAGGCCATGCACCCGTAACCATCAAGAAGACCAGCCCAAATCTCCCTCTTCGCTTCCGCTCCGATGGGACCTTCAAGATTCTCCAGGTGGCTGATATGCATTATGGGAATGGAGGGTTGACTCGGTGTCGAGACGTATTGGACTCCGAGTTTGCGGCATGTTCTGATCGGAACACGTCGCTGTTCCTCAAGAGGATGATTGAAGCTGAAAAGCCTGATTTCATTGCTTTTACAGGTGATAATATATTTGGGCCAAGCTCTACCGACGCTGCTGAATCAATGATTCGAGCATTTGGTCCTGCCATTAAATCAGGCATTCCGTGGGCAGCAGTTTTGGGAAACCACGACCAAGAATCTACAATGAATCGTGAAGAACTCATGTCCTTTATCTCCCTTATGGACTATTCACTTTCACAAGTTAATCCATTAGCTGAAGATCCCTTAGGAGAATGGATGCAAGACATTGATGGTTTTGGCAATTATAATCTGAGAGTATATGGTGCCCCAGGTTCCTACTTGGCAAATAGCAGCATCCTCAACCTTTTCTTTCTTGACAGTGGAGACAGAGAGACTGTTCATGGAGTACGAACTTACGGTTGGATTAAGGAATCACAACTCCGTTGGCTTCGTGGTGTTTCTCAGGGCCACAGTCGAGATCCAGATCACTCAGACAATGTTTTTTCTCCAGATAAACCACCGGCGCTTGCTTTTTTCCATATCCCAATTCCTGAGATCCGGCAGCTGTGGAATAAAGACATTGTTGGCAAGTTTCAGGAGGCTGTGGCATGTTCATCAGTGAACCCGGGAGTTTTGCAGACCCTTGTATCCATGGGAGATGTGAAAGCTGTGTTCATTGGACATGATCATACCAATGACTTTTGTGGGAATCTTGATGGTGTTTGGTTCTGTTATGGTGGGGGCTTTGGATATCATGGTTATGGAAAGACGGGGTGGCCAAGAAGAGCTAGGGTCATATTGGCAGAACTCAGGAAAGGGGAGAAAGGCTGGATGGGAGTTGAGAGGATTAGGACATGGAAGAGTCTTGATGATGAGAAACTGAGCAAGATTGATGAGCAGTTCTTGTGGGAATACCATCTATCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003993 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009987 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0042578 GO:0044237
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos

Protein Analysis

403

Amino Acids

45.35

Weight (kDa)

5.96

Isoelectric Point (pI)

40.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos PF00149 61 - 326 2.5e-15 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015175)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G57140
fragaria_vesca FvH4_4g07080
malus_domestica MD04G1168800.v1.1
prunus_persica Prupe.1G074200_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0399201
rosa_laevigata RLG00000009290
rosa_multiflora Rmu_co8469863.1_g000001 Rmu_sc0002582.1_g000004 Rmu_sc0024868.1_g000004
rosa_roxburghii Rroxscaffold_5G00344160
rosa_rugosa Rorug04G0015100
rosa_samantha Rh4AG093000 Rh4BG090800 Rh4CG101400 Rh4DG085300
rosa_wichuraiana Rw4G007660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 591
AccB1I GGYRCC 1 cut(s) 608
AccBSI CCGCTC 1 cut(s) 168
AciI CCGC 2 cut(s) 166, 263
AclWI GGATC 3 cut(s) 539, 758, 835
AcoI YGGCCR 1 cut(s) 1061
AcuI CTGAAG 1 cut(s) 561
AfaI GTAC 1 cut(s) 693
AflIII ACRYGT 1 cut(s) 282
AgsI TTSAA 2 cut(s) 184, 311
AhdI GACNNNNNGTC 2 cut(s) 10, 234
AjiI CACGTC 1 cut(s) 285
AjnI CCWGG 2 cut(s) 192, 613
AjuI GAANNNNNNNTTGG 2 cut(s) 688, 720
AluBI AGCT 7 cut(s) 20, 314, 366, 539, 850, 953, 1073
AluI AGCT 7 cut(s) 20, 314, 366, 539, 850, 953, 1073
Alw26I GTCTC 3 cut(s) 234, 663, 671
AlwI GGATC 3 cut(s) 539, 758, 835
Ama87I CYCGRG 1 cut(s) 910
AoxI GGCC 4 cut(s) 112, 359, 751, 1061
ApeKI GCWGC 5 cut(s) 20, 377, 434, 636, 847
ArsI GACNNNNNNTTYG 2 cut(s) 242, 274
AspLEI GCGC 1 cut(s) 811
AspS9I GGNCC 4 cut(s) 177, 359, 404, 751
AsuC2I CCSGG 2 cut(s) 911, 912
AsuHPI GGTGA 1 cut(s) 356
AvaI CYCGRG 1 cut(s) 910
AvaII GGWCC 2 cut(s) 177, 404
AxyI CCTNAGG 1 cut(s) 550
BaeGI GKGCMC 1 cut(s) 613
BaeI ACNNNNGTAYC 2 cut(s) 915, 948
BalI TGGCCA 1 cut(s) 1063
BanI GGYRCC 1 cut(s) 608
BbsI GAAGAC 1 cut(s) 143
BbvCI CCTCAGC 1 cut(s) 16
BbvI GCAGC 5 cut(s) 7, 364, 446, 648, 859
BccI CCATC 6 cut(s) 137, 167, 569, 995, 1111, 1209
BciT130I CCWGG 2 cut(s) 194, 615
BciVI GTATCC 1 cut(s) 943
BclI TGATCA 1 cut(s) 970
BcnI CCSGG 2 cut(s) 911, 912
BcoDI GTCTC 3 cut(s) 234, 663, 671
BfaI CTAG 1 cut(s) 1074
BfoI RGCGCY 1 cut(s) 812
BfuI GTATCC 1 cut(s) 943
BisI GCNGC 6 cut(s) 21, 264, 378, 435, 637, 848
BlsI GCNGC 6 cut(s) 22, 265, 379, 436, 638, 849
Bme1390I CCNGG 4 cut(s) 194, 615, 911, 912
Bme18I GGWCC 2 cut(s) 177, 404
BmeRI GACNNNNNGTC 2 cut(s) 10, 234
BmeT110I CYCGRG 1 cut(s) 910
BmgBI CACGTC 1 cut(s) 285
BmgT120I GGNCC 4 cut(s) 177, 359, 404, 751
BmiI GGNNCC 4 cut(s) 32, 178, 610, 619
BmrFI CCNGG 4 cut(s) 194, 615, 911, 912
BmsI GCATC 2 cut(s) 552, 648
BpiI GAAGAC 1 cut(s) 143
BpmI CTGGAG 2 cut(s) 176, 777
Bpu10I CCTNAGC 1 cut(s) 16
BpuEI CTTGAG 1 cut(s) 281
BpuMI CCSGG 2 cut(s) 911, 912
BsaBI GATNNNNATC 1 cut(s) 768
BsaJI CCNNGG 4 cut(s) 428, 613, 910, 936
Bse118I RCCGGY 1 cut(s) 805
Bse1I ACTGG 1 cut(s) 32
Bse21I CCTNAGG 1 cut(s) 550
Bse3DI GCAATG 1 cut(s) 330
Bse8I GATNNNNATC 1 cut(s) 768
BseBI CCWGG 2 cut(s) 194, 615
BseDI CCNNGG 4 cut(s) 428, 613, 910, 936
BseGI GGATG 4 cut(s) 309, 567, 639, 1122
BseJI GATNNNNATC 1 cut(s) 768
BseMI GCAATG 1 cut(s) 330
BseMII CTCAG 7 cut(s) 30, 587, 761, 789, 828, 1108, 1157
BseNI ACTGG 1 cut(s) 32
BseSI GKGCMC 1 cut(s) 613
BseXI GCAGC 5 cut(s) 7, 364, 446, 648, 859
BshFI GGCC 4 cut(s) 114, 361, 753, 1063
BshNI GGYRCC 1 cut(s) 608
BsiHKCI CYCGRG 1 cut(s) 910
BsiSI CCGG 3 cut(s) 806, 844, 911
BslFI GGGAC 1 cut(s) 190
BsmAI GTCTC 3 cut(s) 234, 663, 671
BsmBI CGTCTC 1 cut(s) 234
BsmFI GGGAC 1 cut(s) 190
BsmI GAATGC 1 cut(s) 423
BsnI GGCC 4 cut(s) 114, 361, 753, 1063
BsoBI CYCGRG 1 cut(s) 910
Bsp1286I GDGCHC 1 cut(s) 613
Bsp143I GATC 6 cut(s) 274, 544, 763, 769, 840, 970
Bsp19I CCATGG 1 cut(s) 936
BspACI CCGC 2 cut(s) 166, 263
BspANI GGCC 4 cut(s) 114, 361, 753, 1063
BspCNI CTCAG 7 cut(s) 29, 588, 760, 788, 829, 1107, 1158
BspHI TCATGA 1 cut(s) 1208
BspLI GGNNCC 4 cut(s) 32, 178, 610, 619
BspPI GGATC 3 cut(s) 539, 758, 835
BspQI GCTCTTC 2 cut(s) 72, 1063
BspT107I GGYRCC 1 cut(s) 608
BsrBI CCGCTC 1 cut(s) 168
BsrDI GCAATG 1 cut(s) 330
BsrFI RCCGGY 1 cut(s) 805
BsrI ACTGG 1 cut(s) 32
BssAI RCCGGY 1 cut(s) 805
BssECI CCNNGG 4 cut(s) 428, 613, 910, 936
BssMI GATC 6 cut(s) 274, 544, 763, 769, 840, 970
BssT1I CCWWGG 1 cut(s) 936
Bst2UI CCWGG 2 cut(s) 194, 615
Bst4CI ACNGT 4 cut(s) 665, 682, 704, 758
Bst6I CTCTTC 4 cut(s) 72, 162, 1063, 1139
BstC8I GCNNGC 1 cut(s) 813
BstDEI CTNAG 8 cut(s) 16, 550, 596, 747, 775, 837, 1094, 1166
BstDSI CCRYGG 2 cut(s) 428, 936
BstF5I GGATG 4 cut(s) 309, 567, 639, 1122
BstH2I RGCGCY 1 cut(s) 812
BstHHI GCGC 1 cut(s) 811
BstKTI GATC 6 cut(s) 277, 547, 766, 772, 843, 973
BstMAI GTCTC 3 cut(s) 234, 663, 671
BstMBI GATC 6 cut(s) 274, 544, 763, 769, 840, 970
BstNI CCWGG 2 cut(s) 194, 615
BstNSI RCATGY 2 cut(s) 270, 897
BstSCI CCNGG 4 cut(s) 192, 613, 909, 910
BstSLI GKGCMC 1 cut(s) 613
BstV1I GCAGC 5 cut(s) 7, 364, 446, 648, 859
BstV2I GAAGAC 1 cut(s) 143
BstX2I RGATCY 3 cut(s) 544, 763, 840
BstYI RGATCY 3 cut(s) 544, 763, 840
Bsu36I CCTNAGG 1 cut(s) 550
BsuI GTATCC 1 cut(s) 943
BsuRI GGCC 4 cut(s) 114, 361, 753, 1063
BtgI CCRYGG 2 cut(s) 428, 936
BtrI CACGTC 1 cut(s) 285
BtsCI GGATG 4 cut(s) 309, 567, 639, 1122
BtsIMutI CAGTG 2 cut(s) 670, 909
Cac8I GCNNGC 1 cut(s) 813
CciI TCATGA 1 cut(s) 1208
CfoI GCGC 1 cut(s) 811
Cfr10I RCCGGY 1 cut(s) 805
Cfr13I GGNCC 4 cut(s) 177, 359, 404, 751
Cfr9I CCCGGG 1 cut(s) 910
CseI GACGC 1 cut(s) 383
Csp6I GTAC 1 cut(s) 692
CviQI GTAC 1 cut(s) 692
DdeI CTNAG 8 cut(s) 16, 550, 596, 747, 775, 837, 1094, 1166
DpnI GATC 6 cut(s) 276, 546, 765, 771, 842, 972
DpnII GATC 6 cut(s) 274, 544, 763, 769, 840, 970
DriI GACNNNNNGTC 2 cut(s) 10, 234
EaeI YGGCCR 1 cut(s) 1061
Eam1104I CTCTTC 4 cut(s) 72, 162, 1063, 1139
Eam1105I GACNNNNNGTC 2 cut(s) 10, 234
EarI CTCTTC 4 cut(s) 72, 162, 1063, 1139
Eco130I CCWWGG 1 cut(s) 936
Eco32I GATATC 1 cut(s) 1037
Eco47I GGWCC 2 cut(s) 177, 404
Eco57I CTGAAG 1 cut(s) 561
Eco81I CCTNAGG 1 cut(s) 550
Eco88I CYCGRG 1 cut(s) 910
EcoO109I RGGNCCY 1 cut(s) 177
EcoRII CCWGG 2 cut(s) 192, 613
EcoRV GATATC 1 cut(s) 1037
EcoT14I CCWWGG 1 cut(s) 936
EcoT22I ATGCAT 1 cut(s) 210
ErhI CCWWGG 1 cut(s) 936
Esp3I CGTCTC 1 cut(s) 234
FaqI GGGAC 1 cut(s) 190
FbaI TGATCA 1 cut(s) 970
Fnu4HI GCNGC 6 cut(s) 21, 264, 378, 435, 637, 848
FokI GGATG 4 cut(s) 316, 574, 626, 1129
Fsp4HI GCNGC 6 cut(s) 21, 264, 378, 435, 637, 848
FspBI CTAG 1 cut(s) 1074
GlaI GCGC 1 cut(s) 810
GluI GCNGC 6 cut(s) 21, 264, 378, 435, 637, 848
GsuI CTGGAG 2 cut(s) 176, 777
HaeII RGCGCY 1 cut(s) 812
HaeIII GGCC 4 cut(s) 114, 361, 753, 1063
HapII CCGG 3 cut(s) 806, 844, 911
HgaI GACGC 1 cut(s) 383
HhaI GCGC 1 cut(s) 811
Hin6I GCGC 1 cut(s) 809
HinP1I GCGC 1 cut(s) 809
HincII GTYRAC 1 cut(s) 228
HindII GTYRAC 1 cut(s) 228
HpaII CCGG 3 cut(s) 806, 844, 911
HphI GGTGA 1 cut(s) 356
Hpy166II GTNNAC 2 cut(s) 228, 907
Hpy188I TCNGA 6 cut(s) 172, 256, 274, 279, 597, 778
Hpy8I GTNNAC 2 cut(s) 228, 907
Hpy99I CGWCG 3 cut(s) 13, 289, 377
HpyAV CCTTC 1 cut(s) 190
HpyCH4III ACNGT 4 cut(s) 665, 682, 704, 758
HpyCH4IV ACGT 2 cut(s) 243, 284
HpyCH4V TGCA 5 cut(s) 23, 119, 208, 565, 922
HpyF3I CTNAG 8 cut(s) 16, 550, 596, 747, 775, 837, 1094, 1166
HpySE526I ACGT 2 cut(s) 243, 284
HspAI GCGC 1 cut(s) 809
Ksp22I TGATCA 1 cut(s) 970
Kzo9I GATC 6 cut(s) 274, 544, 763, 769, 840, 970
LguI GCTCTTC 2 cut(s) 72, 1063
LmnI GCTCC 1 cut(s) 173
Lsp1109I GCAGC 5 cut(s) 7, 364, 446, 648, 859
LweI GCATC 2 cut(s) 552, 648
MaeI CTAG 1 cut(s) 1074
MaeII ACGT 2 cut(s) 243, 284
MaeIII GTNAC 1 cut(s) 124
MalI GATC 6 cut(s) 276, 546, 765, 771, 842, 972
MbiI CCGCTC 1 cut(s) 168
MboI GATC 6 cut(s) 274, 544, 763, 769, 840, 970
MboII GAAGA 8 cut(s) 46, 59, 148, 149, 491, 554, 1080, 1156
MflI RGATCY 3 cut(s) 544, 763, 840
MhlI GDGCHC 1 cut(s) 613
MlsI TGGCCA 1 cut(s) 1063
MluCI AATT 2 cut(s) 586, 831
MluNI TGGCCA 1 cut(s) 1063
MlyI GAGTC 3 cut(s) 223, 245, 1156
MmeI TCCRAC 1 cut(s) 686
Mox20I TGGCCA 1 cut(s) 1063
Mph1103I ATGCAT 1 cut(s) 210
MscI TGGCCA 1 cut(s) 1063
MseI TTAA 3 cut(s) 414, 528, 711
MslI CAYNNNNRTG 2 cut(s) 978, 1044
Msp20I TGGCCA 1 cut(s) 1063
MspA1I CMGCKG 2 cut(s) 20, 850
MspI CCGG 3 cut(s) 806, 844, 911
MspR9I CCNGG 4 cut(s) 194, 615, 911, 912
Mva1269I GAATGC 1 cut(s) 423
MvaI CCWGG 2 cut(s) 194, 615
NciI CCSGG 2 cut(s) 911, 912
NcoI CCATGG 1 cut(s) 936
NdeII GATC 6 cut(s) 274, 544, 763, 769, 840, 970
NlaIV GGNNCC 4 cut(s) 32, 178, 610, 619
NsiI ATGCAT 1 cut(s) 210
NspI RCATGY 2 cut(s) 270, 897
PagI TCATGA 1 cut(s) 1208
PciSI GCTCTTC 2 cut(s) 72, 1063
PctI GAATGC 1 cut(s) 423
PfeI GAWTC 7 cut(s) 187, 383, 391, 461, 472, 716, 994
PkrI GCNGC 6 cut(s) 22, 265, 379, 436, 638, 849
PleI GAGTC 3 cut(s) 223, 245, 1155
PpsI GAGTC 3 cut(s) 223, 245, 1155
PpuMI RGGWCCY 1 cut(s) 177
PsiI TTATAA 1 cut(s) 591
Psp5II RGGWCCY 1 cut(s) 177
Psp6I CCWGG 2 cut(s) 192, 613
PspGI CCWGG 2 cut(s) 192, 613
PspN4I GGNNCC 4 cut(s) 32, 178, 610, 619
PspPI GGNCC 4 cut(s) 177, 359, 404, 751
PspPPI RGGWCCY 1 cut(s) 177
PsuI RGATCY 3 cut(s) 544, 763, 840
PvuII CAGCTG 2 cut(s) 20, 850
RsaI GTAC 1 cut(s) 693
RsaNI GTAC 1 cut(s) 692
RseI CAYNNNNRTG 2 cut(s) 978, 1044
SapI GCTCTTC 2 cut(s) 72, 1063
SaqAI TTAA 3 cut(s) 414, 528, 711
SatI GCNGC 6 cut(s) 21, 264, 378, 435, 637, 848
Sau3AI GATC 6 cut(s) 274, 544, 763, 769, 840, 970
Sau96I GGNCC 4 cut(s) 177, 359, 404, 751
SchI GAGTC 3 cut(s) 223, 245, 1156
ScrFI CCNGG 4 cut(s) 194, 615, 911, 912
SduI GDGCHC 1 cut(s) 613
SfaNI GCATC 2 cut(s) 552, 648
SgrAI CRCCGGYG 1 cut(s) 805
SinI GGWCC 2 cut(s) 177, 404
SmaI CCCGGG 1 cut(s) 912
SmiMI CAYNNNNRTG 2 cut(s) 978, 1044
SmlI CTYRAG 1 cut(s) 296
SmoI CTYRAG 1 cut(s) 296
Sse9I AATT 2 cut(s) 586, 831
SsiI CCGC 2 cut(s) 166, 263
SspMI CTAG 1 cut(s) 1074
StyD4I CCNGG 4 cut(s) 192, 613, 909, 910
StyI CCWWGG 1 cut(s) 936
TaaI ACNGT 4 cut(s) 665, 682, 704, 758
TaiI ACGT 2 cut(s) 246, 287
TaqI TCGA 3 cut(s) 238, 394, 760
TasI AATT 2 cut(s) 586, 831
TauI GCSGC 1 cut(s) 266
TfiI GAWTC 7 cut(s) 187, 383, 391, 461, 472, 716, 994
Tru1I TTAA 3 cut(s) 414, 528, 711
Tru9I TTAA 3 cut(s) 414, 528, 711
TscAI CASTG 2 cut(s) 670, 909
TseI GCWGC 5 cut(s) 20, 377, 434, 636, 847
TspDTI ATGAA 6 cut(s) 46, 319, 485, 674, 888, 949
TspGWI ACGGA 2 cut(s) 417, 716
TspMI CCCGGG 1 cut(s) 910
TspRI CASTG 2 cut(s) 670, 909
VpaK11BI GGWCC 2 cut(s) 177, 404
XceI RCATGY 2 cut(s) 270, 897
XmaI CCCGGG 1 cut(s) 910
XspI CTAG 1 cut(s) 1074
Zsp2I ATGCAT 1 cut(s) 210
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.