MD04G1168800.v1.1
NAC Family

inactive purple acid phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0015175
Physical Location & Seq
Reverse (-)
25924849 .. 25928095
3247 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1168800.v1.1.491

Sequence Viewer

Length: 1353 bp
ATGGAATCGCCGTCCTCGGCAGCAAACTGGAAATACCAGACGCTGTCCGAAGATGTTGACTCTTCAGTGGAATCGTCGTCCTCGGCAGCAAACTGGAAACACTCGGTCCTGTATTTGTCTTTCCTCTGCTCAGTCATCTACTTTTTTCACACCCAAATCTCTCACAATTTGCTAATCGGCCATCGGCCTGTCAGAGTAAAAAAAACCAGCCCAGATCTTCCCCTTCGTTTCCGGTCCGACGGAACCTTCAAAATCCTGCAGGTGGCTGATATGCATTACGGGAATGGAAGGTTGACTCGCTGCCGAGATGTGTTGGACTCCGAGTTTGACTGGTGTTCTGATCTCAACACCTCCCACTTCCTGAGGAAGATGATTGAAGCTGAGAAGCCTCATTTCATTGCTTTTACAGGAGATAATATATTCGGGTCAAGCTCTGTTGATGCTGCTGAATCCATGCTTAGAGCATTTGGTCCTGCCATTGATTCAGGAGTTCCGTGGGCAGCAGTTTTAGGAAACCACGACCAAGAATCTACGATGACACGTGAGGAACTGATGTCTTTTATCTCACTTATGGATTATTCAGTTTCACAAGTTAATCCATTAGCTGAGGATCTCTCTAAAGGAGGAAGCGCGAAAAACATTGATGGATTTGGCAATTATGATCTGAGAGTATATGGTGCCCCGGGATCCTATTTGGCAAACACAAGCATCCTCAATCTTTTCTTTCTTGACAGTGGAGATAGGGAAACTGTTGAAGGAGTTCAAACTTATGGTTGGATTAAGGAATCTCAACTCCATTGGCTTCGTGACATTTCTCAGGGATTTCAGGGCCACATTGGATATCTAGATCGCTCAGCAGAGGCTTTTCCTCCGGACAAACCACCAGCGCTTGTATTTTTTCATATCCCAATTCCAGAGGTCCGGCAGCTGTGGCACAAAAAGATTGTTGGCCAGTTTCAGGAGGCTGTGGCATGTTCAAAAGTGAACTCAGGAGTTTTGCAGACCCTTGTATCCATGGGAGACGTGAAAGCCGTGTTCATGGGACACGATCATACCAATGACTTCTGCGGGAATCTTGATGGTATTTGGTTTTGTTATGGTGGGGGCTTCGGGTACCATGGTTATGGAAACGCTAGGTGGCCAAGGAGAGCAAGGGTCATATTGGCAGAACTTGGGAAGGGAAAGAAAGGCTGGATGGGCGTTGAGAGGATTAAGACATGGAAGCGTCTTGACGACGAGAAACTGAGCAAGATTGATGAGCAAGCACTAGCAGAGGAACTGCCCATATGCCCTCACGAGTTTTTCCGTACACTGCTTGATTTAGCATCAGAGAGGAGAGGACTGTTTATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003993 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009987 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0042578 GO:0044237
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metallophos

Protein Analysis

451

Amino Acids

50.56

Weight (kDa)

5.98

Isoelectric Point (pI)

44.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metallophos PF00149 83 - 352 6.8e-13 Calcineurin-like phosphoesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015175)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G57140
fragaria_vesca FvH4_4g07080
malus_domestica MD04G1168800.v1.1
prunus_persica Prupe.1G074200_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0399201
rosa_laevigata RLG00000009290
rosa_multiflora Rmu_co8469863.1_g000001 Rmu_sc0002582.1_g000004 Rmu_sc0024868.1_g000004
rosa_roxburghii Rroxscaffold_5G00344160
rosa_rugosa Rorug04G0015100
rosa_samantha Rh4AG093000 Rh4BG090800 Rh4CG101400 Rh4DG085300
rosa_wichuraiana Rw4G007660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 250
Acc36I ACCTGC 1 cut(s) 250
Acc65I GGTACC 1 cut(s) 1113
AccB1I GGYRCC 2 cut(s) 677, 1113
AccII CGCG 1 cut(s) 632
AccIII TCCGGA 1 cut(s) 871
AciI CCGC 1 cut(s) 1068
AclWI GGATC 3 cut(s) 618, 681, 694
AcoI YGGCCR 3 cut(s) 178, 949, 1139
AcuI CTGAAG 1 cut(s) 48
AcvI CACGTG 1 cut(s) 542
AfaI GTAC 2 cut(s) 1115, 1309
AfeI AGCGCT 1 cut(s) 888
AfiI CCNNNNNNNGG 2 cut(s) 262, 958
AflIII ACRYGT 1 cut(s) 539
AgsI TTSAA 5 cut(s) 250, 377, 755, 764, 978
AjiI CACGTC 1 cut(s) 1024
AluBI AGCT 4 cut(s) 380, 432, 605, 928
AluI AGCT 4 cut(s) 380, 432, 605, 928
Alw26I GTCTC 1 cut(s) 1014
AlwI GGATC 3 cut(s) 618, 681, 694
AlwNI CAGNNNCTG 1 cut(s) 43
Ama87I CYCGRG 1 cut(s) 682
Aor13HI TCCGGA 1 cut(s) 871
Aor51HI AGCGCT 1 cut(s) 888
AoxI GGCC 5 cut(s) 178, 185, 829, 949, 1139
ApeKI GCWGC 6 cut(s) 20, 86, 300, 443, 500, 925
ArsI GACNNNNNNTTYG 2 cut(s) 308, 340
Asp700I GAANNNNTTC 1 cut(s) 759
Asp718I GGTACC 1 cut(s) 1113
AspLEI GCGC 2 cut(s) 632, 889
AspS9I GGNCC 5 cut(s) 106, 234, 470, 829, 919
AsuC2I CCSGG 2 cut(s) 683, 684
AvaI CYCGRG 1 cut(s) 682
AvaII GGWCC 4 cut(s) 106, 234, 470, 919
AxyI CCTNAGG 1 cut(s) 362
BaeGI GKGCMC 1 cut(s) 682
BaeI ACNNNNGTAYC 4 cut(s) 993, 1026, 1105, 1138
BalI TGGCCA 2 cut(s) 951, 1141
BamHI GGATCC 1 cut(s) 686
BanI GGYRCC 2 cut(s) 677, 1113
BauI CACGAG 1 cut(s) 1295
BbrPI CACGTG 1 cut(s) 542
BbvCI CCTCAGC 1 cut(s) 606
BbvI GCAGC 6 cut(s) 32, 98, 287, 430, 512, 937
BccI CCATC 4 cut(s) 189, 638, 1073, 1189
BceAI ACGGC 1 cut(s) 1016
BciVI GTATCC 1 cut(s) 1021
BcnI CCSGG 2 cut(s) 683, 684
BcoDI GTCTC 1 cut(s) 1014
BfaI CTAG 3 cut(s) 845, 1134, 1268
BfmI CTRYAG 1 cut(s) 257
BfoI RGCGCY 1 cut(s) 890
BfuAI ACCTGC 1 cut(s) 250
BfuI GTATCC 1 cut(s) 1021
BglII AGATCT 1 cut(s) 214
BisI GCNGC 6 cut(s) 21, 87, 301, 444, 501, 926
BlpI GCTNAGC 1 cut(s) 853
BlsI GCNGC 6 cut(s) 22, 88, 302, 445, 502, 927
Bme1390I CCNGG 2 cut(s) 683, 684
Bme18I GGWCC 4 cut(s) 106, 234, 470, 919
BmeT110I CYCGRG 1 cut(s) 682
BmgBI CACGTC 1 cut(s) 1024
BmgT120I GGNCC 5 cut(s) 106, 234, 470, 829, 919
BmiI GGNNCC 4 cut(s) 244, 679, 688, 1115
BmrFI CCNGG 2 cut(s) 683, 684
BmsI GCATC 3 cut(s) 430, 717, 1334
BplI GAGNNNNNCTC 2 cut(s) 599, 631
Bpu10I CCTNAGC 1 cut(s) 606
Bpu1102I GCTNAGC 1 cut(s) 853
BpuMI CCSGG 2 cut(s) 683, 684
BsaAI YACGTR 1 cut(s) 542
BsaJI CCNNGG 8 cut(s) 15, 81, 494, 681, 682, 1014, 1117, 1142
BsaWI WCCGGW 2 cut(s) 231, 871
Bsc4I CCNNNNNNNGG 2 cut(s) 262, 958
Bse1I ACTGG 4 cut(s) 32, 98, 335, 952
Bse21I CCTNAGG 1 cut(s) 362
Bse3DI GCAATG 1 cut(s) 396
BseAI TCCGGA 1 cut(s) 871
BseDI CCNNGG 8 cut(s) 15, 81, 494, 681, 682, 1014, 1117, 1142
BseGI GGATG 2 cut(s) 708, 1200
BseLI CCNNNNNNNGG 2 cut(s) 262, 958
BseMI GCAATG 1 cut(s) 396
BseMII CTCAG 9 cut(s) 144, 353, 372, 597, 656, 830, 867, 1002, 1235
BseNI ACTGG 4 cut(s) 32, 98, 335, 952
BseRI GAGGAG 1 cut(s) 1348
BseSI GKGCMC 1 cut(s) 682
BseXI GCAGC 6 cut(s) 32, 98, 287, 430, 512, 937
Bsh1236I CGCG 1 cut(s) 632
BshFI GGCC 5 cut(s) 180, 187, 831, 951, 1141
BshNI GGYRCC 2 cut(s) 677, 1113
BsiHKCI CYCGRG 1 cut(s) 682
BsiSI CCGG 4 cut(s) 232, 683, 872, 922
BslFI GGGAC 1 cut(s) 1056
BslI CCNNNNNNNGG 2 cut(s) 262, 958
BsmAI GTCTC 1 cut(s) 1014
BsmBI CGTCTC 1 cut(s) 1014
BsmFI GGGAC 1 cut(s) 1056
BsnI GGCC 5 cut(s) 180, 187, 831, 951, 1141
BsoBI CYCGRG 1 cut(s) 682
Bsp1286I GDGCHC 1 cut(s) 682
Bsp13I TCCGGA 1 cut(s) 871
Bsp143I GATC 7 cut(s) 214, 340, 610, 661, 686, 847, 1048
Bsp1720I GCTNAGC 1 cut(s) 853
Bsp19I CCATGG 2 cut(s) 1014, 1117
BspACI CCGC 1 cut(s) 1068
BspANI GGCC 5 cut(s) 180, 187, 831, 951, 1141
BspCNI CTCAG 9 cut(s) 143, 354, 373, 598, 657, 829, 866, 1001, 1236
BspEI TCCGGA 1 cut(s) 871
BspFNI CGCG 1 cut(s) 632
BspLI GGNNCC 4 cut(s) 244, 679, 688, 1115
BspMAI CTGCAG 1 cut(s) 261
BspMI ACCTGC 1 cut(s) 250
BspPI GGATC 3 cut(s) 618, 681, 694
BspT107I GGYRCC 2 cut(s) 677, 1113
BsrDI GCAATG 1 cut(s) 396
BsrI ACTGG 4 cut(s) 32, 98, 335, 952
BssECI CCNNGG 8 cut(s) 15, 81, 494, 681, 682, 1014, 1117, 1142
BssMI GATC 7 cut(s) 214, 340, 610, 661, 686, 847, 1048
BssSI CACGAG 1 cut(s) 1295
BssT1I CCWWGG 3 cut(s) 1014, 1117, 1142
Bst2BI CACGAG 1 cut(s) 1295
Bst4CI ACNGT 3 cut(s) 734, 751, 1344
Bst6I CTCTTC 1 cut(s) 67
BstBAI YACGTR 1 cut(s) 542
BstC8I GCNNGC 1 cut(s) 1263
BstDSI CCRYGG 3 cut(s) 494, 1014, 1117
BstF5I GGATG 2 cut(s) 708, 1200
BstFNI CGCG 1 cut(s) 632
BstH2I RGCGCY 1 cut(s) 890
BstHHI GCGC 2 cut(s) 632, 889
BstKTI GATC 7 cut(s) 217, 343, 613, 664, 689, 850, 1051
BstMAI GTCTC 1 cut(s) 1014
BstMBI GATC 7 cut(s) 214, 340, 610, 661, 686, 847, 1048
BstMWI GCNNNNNNNGC 2 cut(s) 931, 1197
BstNSI RCATGY 1 cut(s) 975
BstSCI CCNGG 2 cut(s) 681, 682
BstSFI CTRYAG 1 cut(s) 257
BstSLI GKGCMC 1 cut(s) 682
BstUI CGCG 1 cut(s) 632
BstV1I GCAGC 6 cut(s) 32, 98, 287, 430, 512, 937
BstX2I RGATCY 3 cut(s) 214, 610, 686
BstXI CCANNNNNNTGG 1 cut(s) 1124
BstYI RGATCY 3 cut(s) 214, 610, 686
Bsu36I CCTNAGG 1 cut(s) 362
BsuI GTATCC 1 cut(s) 1021
BsuRI GGCC 5 cut(s) 180, 187, 831, 951, 1141
BtgI CCRYGG 3 cut(s) 494, 1014, 1117
BtrI CACGTC 1 cut(s) 1024
BtsCI GGATG 2 cut(s) 708, 1200
BtsI GCAGTG 1 cut(s) 1310
BtsIMutI CAGTG 3 cut(s) 72, 739, 1310
BveI ACCTGC 1 cut(s) 250
Cac8I GCNNGC 1 cut(s) 1263
CaiI CAGNNNCTG 1 cut(s) 43
CfoI GCGC 2 cut(s) 632, 889
Cfr13I GGNCC 5 cut(s) 106, 234, 470, 829, 919
Cfr9I CCCGGG 1 cut(s) 682
CpoI CGGWCCG 1 cut(s) 234
CseI GACGC 2 cut(s) 49, 1214
Csp6I GTAC 2 cut(s) 1114, 1308
CspI CGGWCCG 1 cut(s) 234
CviAII CATG 6 cut(s) 454, 972, 1015, 1039, 1118, 1218
CviQI GTAC 2 cut(s) 1114, 1308
DpnI GATC 7 cut(s) 216, 342, 612, 663, 688, 849, 1050
DpnII GATC 7 cut(s) 214, 340, 610, 661, 686, 847, 1048
EaeI YGGCCR 3 cut(s) 178, 949, 1139
Eam1104I CTCTTC 1 cut(s) 67
EarI CTCTTC 1 cut(s) 67
Eco130I CCWWGG 3 cut(s) 1014, 1117, 1142
Eco32I GATATC 1 cut(s) 842
Eco47I GGWCC 4 cut(s) 106, 234, 470, 919
Eco47III AGCGCT 1 cut(s) 888
Eco57I CTGAAG 1 cut(s) 48
Eco72I CACGTG 1 cut(s) 542
Eco81I CCTNAGG 1 cut(s) 362
Eco88I CYCGRG 1 cut(s) 682
EcoRV GATATC 1 cut(s) 842
EcoT14I CCWWGG 3 cut(s) 1014, 1117, 1142
EcoT22I ATGCAT 1 cut(s) 276
ErhI CCWWGG 3 cut(s) 1014, 1117, 1142
Esp3I CGTCTC 1 cut(s) 1014
FaeI CATG 6 cut(s) 457, 975, 1018, 1042, 1121, 1221
FaqI GGGAC 1 cut(s) 1056
FatI CATG 6 cut(s) 453, 971, 1014, 1038, 1117, 1217
FauI CCCGC 1 cut(s) 1061
FauNDI CATATG 1 cut(s) 1286
Fnu4HI GCNGC 6 cut(s) 21, 87, 301, 444, 501, 926
FokI GGATG 2 cut(s) 695, 1207
Fsp4HI GCNGC 6 cut(s) 21, 87, 301, 444, 501, 926
FspBI CTAG 3 cut(s) 845, 1134, 1268
GlaI GCGC 2 cut(s) 631, 888
GluI GCNGC 6 cut(s) 21, 87, 301, 444, 501, 926
HaeII RGCGCY 1 cut(s) 890
HaeIII GGCC 5 cut(s) 180, 187, 831, 951, 1141
HapII CCGG 4 cut(s) 232, 683, 872, 922
HgaI GACGC 2 cut(s) 49, 1214
HhaI GCGC 2 cut(s) 632, 889
Hin1II CATG 6 cut(s) 457, 975, 1018, 1042, 1121, 1221
Hin6I GCGC 2 cut(s) 630, 887
HinP1I GCGC 2 cut(s) 630, 887
HincII GTYRAC 2 cut(s) 58, 294
HindII GTYRAC 2 cut(s) 58, 294
HpaII CCGG 4 cut(s) 232, 683, 872, 922
Hpy166II GTNNAC 4 cut(s) 58, 294, 985, 1310
Hpy188I TCNGA 7 cut(s) 49, 194, 238, 322, 340, 666, 1330
Hpy8I GTNNAC 4 cut(s) 58, 294, 985, 1310
Hpy99I CGWCG 3 cut(s) 79, 242, 1238
HpyAV CCTTC 5 cut(s) 233, 256, 282, 749, 1171
HpyCH4III ACNGT 3 cut(s) 734, 751, 1344
HpyCH4IV ACGT 2 cut(s) 541, 1023
HpyCH4V TGCA 3 cut(s) 259, 274, 1000
HpyF10VI GCNNNNNNNGC 2 cut(s) 931, 1197
HpySE526I ACGT 2 cut(s) 541, 1023
Hsp92II CATG 6 cut(s) 457, 975, 1018, 1042, 1121, 1221
HspAI GCGC 2 cut(s) 630, 887
Kpn2I TCCGGA 1 cut(s) 871
KpnI GGTACC 1 cut(s) 1117
Kzo9I GATC 7 cut(s) 214, 340, 610, 661, 686, 847, 1048
Lsp1109I GCAGC 6 cut(s) 32, 98, 287, 430, 512, 937
LweI GCATC 3 cut(s) 430, 717, 1334
MaeI CTAG 3 cut(s) 845, 1134, 1268
MaeII ACGT 2 cut(s) 541, 1023
MaeIII GTNAC 1 cut(s) 806
MalI GATC 7 cut(s) 216, 342, 612, 663, 688, 849, 1050
MboI GATC 7 cut(s) 214, 340, 610, 661, 686, 847, 1048
MboII GAAGA 4 cut(s) 54, 62, 209, 379
MflI RGATCY 3 cut(s) 214, 610, 686
MhlI GDGCHC 1 cut(s) 682
MlsI TGGCCA 2 cut(s) 951, 1141
MluCI AATT 3 cut(s) 166, 655, 909
MluNI TGGCCA 2 cut(s) 951, 1141
MlyI GAGTC 3 cut(s) 53, 289, 311
MmeI TCCRAC 3 cut(s) 261, 294, 755
Mox20I TGGCCA 2 cut(s) 951, 1141
Mph1103I ATGCAT 1 cut(s) 276
MroI TCCGGA 1 cut(s) 871
MroXI GAANNNNTTC 1 cut(s) 759
MscI TGGCCA 2 cut(s) 951, 1141
MseI TTAA 3 cut(s) 594, 780, 1212
MslI CAYNNNNRTG 2 cut(s) 1056, 1122
Msp20I TGGCCA 2 cut(s) 951, 1141
MspA1I CMGCKG 1 cut(s) 928
MspI CCGG 4 cut(s) 232, 683, 872, 922
MspR9I CCNGG 2 cut(s) 683, 684
MvnI CGCG 1 cut(s) 632
MwoI GCNNNNNNNGC 2 cut(s) 931, 1197
NciI CCSGG 2 cut(s) 683, 684
NcoI CCATGG 2 cut(s) 1014, 1117
NdeI CATATG 1 cut(s) 1286
NdeII GATC 7 cut(s) 214, 340, 610, 661, 686, 847, 1048
NlaIII CATG 6 cut(s) 457, 975, 1018, 1042, 1121, 1221
NlaIV GGNNCC 4 cut(s) 244, 679, 688, 1115
NmeAIII GCCGAG 2 cut(s) 62, 329
NmuCI GTSAC 1 cut(s) 806
NsiI ATGCAT 1 cut(s) 276
NspI RCATGY 1 cut(s) 975
PaqCI CACCTGC 1 cut(s) 250
PcsI WCGNNNNNNNCGW 1 cut(s) 1029
PdmI GAANNNNTTC 1 cut(s) 759
PfeI GAWTC 7 cut(s) 5, 71, 449, 482, 527, 785, 1072
PflFI GACNNNGTC 1 cut(s) 43
PkrI GCNGC 6 cut(s) 22, 88, 302, 445, 502, 927
PleI GAGTC 3 cut(s) 53, 289, 311
PmaCI CACGTG 1 cut(s) 542
PmlI CACGTG 1 cut(s) 542
PpsI GAGTC 3 cut(s) 53, 289, 311
Ppu21I YACGTR 1 cut(s) 542
PspCI CACGTG 1 cut(s) 542
PspN4I GGNNCC 4 cut(s) 244, 679, 688, 1115
PspPI GGNCC 5 cut(s) 106, 234, 470, 829, 919
PstI CTGCAG 1 cut(s) 261
PstNI CAGNNNCTG 1 cut(s) 43
PsuI RGATCY 3 cut(s) 214, 610, 686
PsyI GACNNNGTC 1 cut(s) 43
PvuII CAGCTG 1 cut(s) 928
RsaI GTAC 2 cut(s) 1115, 1309
RsaNI GTAC 2 cut(s) 1114, 1308
RseI CAYNNNNRTG 2 cut(s) 1056, 1122
Rsr2I CGGWCCG 1 cut(s) 234
RsrII CGGWCCG 1 cut(s) 234
SaqAI TTAA 3 cut(s) 594, 780, 1212
SatI GCNGC 6 cut(s) 21, 87, 301, 444, 501, 926
Sau3AI GATC 7 cut(s) 214, 340, 610, 661, 686, 847, 1048
Sau96I GGNCC 5 cut(s) 106, 234, 470, 829, 919
SbfI CCTGCAGG 1 cut(s) 261
SchI GAGTC 3 cut(s) 53, 289, 311
ScrFI CCNGG 2 cut(s) 683, 684
SdaI CCTGCAGG 1 cut(s) 261
SduI GDGCHC 1 cut(s) 682
SfaNI GCATC 3 cut(s) 430, 717, 1334
SfcI CTRYAG 1 cut(s) 257
SinI GGWCC 4 cut(s) 106, 234, 470, 919
SmaI CCCGGG 1 cut(s) 684
SmiMI CAYNNNNRTG 2 cut(s) 1056, 1122
Sse8387I CCTGCAGG 1 cut(s) 261
Sse9I AATT 3 cut(s) 166, 655, 909
SsiI CCGC 1 cut(s) 1068
SspMI CTAG 3 cut(s) 845, 1134, 1268
StyD4I CCNGG 2 cut(s) 681, 682
StyI CCWWGG 3 cut(s) 1014, 1117, 1142
TaaI ACNGT 3 cut(s) 734, 751, 1344
TaiI ACGT 2 cut(s) 544, 1026
TaqII GACCGA 1 cut(s) 94
TasI AATT 3 cut(s) 166, 655, 909
TfiI GAWTC 7 cut(s) 5, 71, 449, 482, 527, 785, 1072
Tru1I TTAA 3 cut(s) 594, 780, 1212
Tru9I TTAA 3 cut(s) 594, 780, 1212
TscAI CASTG 3 cut(s) 72, 739, 1317
TseFI GTSAC 1 cut(s) 806
TseI GCWGC 6 cut(s) 20, 86, 300, 443, 500, 925
Tsp45I GTSAC 1 cut(s) 806
TspDTI ATGAA 3 cut(s) 385, 890, 1027
TspGWI ACGGA 3 cut(s) 255, 483, 1295
TspMI CCCGGG 1 cut(s) 682
TspRI CASTG 3 cut(s) 72, 739, 1317
Tth111I GACNNNGTC 1 cut(s) 43
VpaK11BI GGWCC 4 cut(s) 106, 234, 470, 919
XbaI TCTAGA 1 cut(s) 844
XceI RCATGY 1 cut(s) 975
XmaI CCCGGG 1 cut(s) 682
XmnI GAANNNNTTC 1 cut(s) 759
XspI CTAG 3 cut(s) 845, 1134, 1268
Zsp2I ATGCAT 1 cut(s) 276
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.