Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0010999

Physical Location & Seq

Genomic Coordinates Reverse (-)

24336623 .. 24338802

Gene Structure 2180 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G60540.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 768 bp

ATGACCGTCGGAGTTTTAGCTTTGCAAGGTTCTTTCAATGAGCACATCGCGGCTCTGCGGCGGCTCGGTGTCCAAGGCGTCGAGATTAGGAAGGCTGACCAGCTTCTCACCGTTTCTTCTCTTATCATTCCTGGCGGCGAGAGCACCACCATGGCCAAACTCGCCGAGTATCATAACTTGTTTCCGGCTCTACGTGAGTTTGTTAAGATGGGGAAACCTGTTTGGGGGACATGCGCAGGTCTTATATTCTTGGCAGACAGAGCAGTTGGTCAGAAAGAGGGAGGTCAGGAATTAGTTGGTGGCCTTGATTGCACCGTACATAGGAACTTCTTCGGTAGCCAGATTCAAAGTTTTGAAGCTGATATCTTAGTACCTCAACTAACATCTCAAGAAGGTGGGCCAGAGACATACAGGGGAGTGTTCATACGTGCTCCAGCTGTTCTTGATGTAGGTCCTGATGTCGAAGTCCTGGCGGATTATCCCGTCCCATCAAACAAGGTCTTGTATTCAAGCTCCACCGTACAAATTCAAGAGGAAGATGCTCTTCCTGAAACAAAAGTCATTGTTGCTGTGAAGCAAGGAAACTTGTTAGCAACTGCTTTTCATCCCGAGCTTACTGCAGACACTCGATGGCACAGTTATTTCATAAAGATGACGAAAGAGATTGAGCAAGGAGCTTCTTCAAGCAGTAGTAAGACTATTGTATCTGTTGGAGAAACAAGTGCTGGTCCCGAGCCAGCTAAGCCTGATCTTCCTATATTTCAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004359 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006081 GO:0006725 GO:0006732 GO:0006766 GO:0006767 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008614 GO:0009058 GO:0009108 GO:0009110 GO:0009987 GO:0016787 GO:0016810 GO:0016811 GO:0017144 GO:0018130 GO:0019438 GO:0019637 GO:0032991 GO:0034641 GO:0042364 GO:0042816 GO:0042819 GO:0042822 GO:0042823 GO:0044237 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046184 GO:0046483 GO:0046982 GO:0046983 GO:0051186 GO:0051188 GO:0071704 GO:0072524 GO:0072525 GO:0090407 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901615 GO:1901617 GO:1902494 GO:1903600

KEGG Pathways

Metabolic & Signaling

ko00750 map00750

Pfam Domains

Protein Families

SNO

Protein Analysis

255

Amino Acids

27.44

Weight (kDa)

5.18

Isoelectric Point (pI)

49.72

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SNO	PF01174	5 - 219	3.2e-49	SNO glutamine amidotransferase family
GATase_3	PF07685	15 - 105	4.6e-08	CobB/CobQ-like glutamine amidotransferase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010999)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G60540
fragaria_vesca	FvH4_3g32570 FvH4_3g32570 FvH4_3g32570 FvH4_3g32570 FvH4_3g32570 FvH4_3g32570
malus_domestica	MD03G1113000.v1.1 MD11G1133800.v1.1
prunus_persica	Prupe.6G100100_v2.0.a1
pyrus_communis	pycom11g11030
rosa_chinensis	RchiOBHm_Chr5g0059641
rosa_laevigata	RLG00000035317
rosa_multiflora	Rmu_sc0001146.1_g000002
rosa_roxburghii	Rroxscaffold_1G00020970
rosa_rugosa	Rorug05G0323800
rosa_samantha	Rh5AG389100 Rh5BG400800 Rh5DG417200
rosa_wichuraiana	Rw5G036630

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	235
Acc36I	ACCTGC	1 cut(s)	227
AccII	CGCG	1 cut(s)	50
AciI	CCGC	5 cut(s)	50, 58, 61, 135, 473
AcoI	YGGCCR	1 cut(s)	153
AcsI	RAATTY	1 cut(s)	525
AcyI	GRCGYC	1 cut(s)	78
AfaI	GTAC	3 cut(s)	318, 372, 522
AfiI	CCNNNNNNNGG	2 cut(s)	224, 321
AgsI	TTSAA	7 cut(s)	37, 347, 356, 510, 530, 684, 764
AjnI	CCWGG	2 cut(s)	130, 468
AluBI	AGCT	8 cut(s)	20, 103, 359, 437, 513, 613, 677, 740
AluI	AGCT	8 cut(s)	20, 103, 359, 437, 513, 613, 677, 740
Alw21I	GWGCWC	3 cut(s)	45, 146, 433
Alw26I	GTCTC	1 cut(s)	398
Ama87I	CYCGRG	2 cut(s)	608, 731
AoxI	GGCC	3 cut(s)	153, 301, 398
ApoI	RAATTY	1 cut(s)	525
Asp700I	GAANNNNTTC	1 cut(s)	329
AspLEI	GCGC	1 cut(s)	236
AspS9I	GGNCC	3 cut(s)	398, 452, 728
AsuHPI	GGTGA	1 cut(s)	100
AvaI	CYCGRG	2 cut(s)	608, 731
AvaII	GGWCC	2 cut(s)	452, 728
BaeI	ACNNNNGTAYC	2 cut(s)	687, 720
BalI	TGGCCA	1 cut(s)	155
Bbv12I	GWGCWC	3 cut(s)	45, 146, 433
BccI	CCATC	3 cut(s)	202, 496, 624
BcgI	CGANNNNNNTGC	2 cut(s)	599, 633
BciT130I	CCWGG	2 cut(s)	132, 470
BcoDI	GTCTC	1 cut(s)	398
BfmI	CTRYAG	1 cut(s)	618
BfuAI	ACCTGC	1 cut(s)	227
BisI	GCNGC	4 cut(s)	51, 59, 62, 136
BlpI	GCTNAGC	1 cut(s)	741
BlsI	GCNGC	4 cut(s)	52, 60, 63, 137
Bme1390I	CCNGG	2 cut(s)	132, 470
Bme18I	GGWCC	2 cut(s)	452, 728
BmeT110I	CYCGRG	2 cut(s)	608, 731
BmgT120I	GGNCC	3 cut(s)	398, 452, 728
BmiI	GGNNCC	1 cut(s)	730
BmrFI	CCNGG	2 cut(s)	132, 470
BmsI	GCATC	1 cut(s)	529
BpmI	CTGGAG	1 cut(s)	417
Bpu1102I	GCTNAGC	1 cut(s)	741
BpuEI	CTTGAG	1 cut(s)	372
BsaAI	YACGTR	2 cut(s)	194, 428
BsaHI	GRCGYC	1 cut(s)	78
BsaJI	CCNNGG	2 cut(s)	73, 150
Bsc4I	CCNNNNNNNGG	2 cut(s)	224, 321
BseBI	CCWGG	2 cut(s)	132, 470
BseDI	CCNNGG	2 cut(s)	73, 150
BseGI	GGATG	1 cut(s)	604
BseLI	CCNNNNNNNGG	2 cut(s)	224, 321
Bsh1236I	CGCG	1 cut(s)	50
BshFI	GGCC	3 cut(s)	155, 303, 400
BsiHKAI	GWGCWC	3 cut(s)	45, 146, 433
BsiHKCI	CYCGRG	2 cut(s)	608, 731
BsiSI	CCGG	1 cut(s)	185
BslFI	GGGAC	3 cut(s)	241, 470, 714
BslI	CCNNNNNNNGG	2 cut(s)	224, 321
BsmAI	GTCTC	1 cut(s)	398
BsmFI	GGGAC	3 cut(s)	241, 470, 714
BsnI	GGCC	3 cut(s)	155, 303, 400
BsoBI	CYCGRG	2 cut(s)	608, 731
Bsp1286I	GDGCHC	3 cut(s)	45, 146, 433
Bsp143I	GATC	1 cut(s)	748
Bsp1720I	GCTNAGC	1 cut(s)	741
Bsp19I	CCATGG	1 cut(s)	150
BspACI	CCGC	5 cut(s)	50, 58, 61, 135, 473
BspANI	GGCC	3 cut(s)	155, 303, 400
BspFNI	CGCG	1 cut(s)	50
BspLI	GGNNCC	1 cut(s)	730
BspMAI	CTGCAG	1 cut(s)	622
BspMI	ACCTGC	1 cut(s)	227
BspQI	GCTCTTC	1 cut(s)	549
BssECI	CCNNGG	2 cut(s)	73, 150
BssMI	GATC	1 cut(s)	748
BssNI	GRCGYC	1 cut(s)	78
BssT1I	CCWWGG	2 cut(s)	73, 150
Bst2UI	CCWGG	2 cut(s)	132, 470
Bst4CI	ACNGT	5 cut(s)	7, 112, 316, 520, 638
Bst6I	CTCTTC	1 cut(s)	549
BstACI	GRCGYC	1 cut(s)	78
BstBAI	YACGTR	2 cut(s)	194, 428
BstC8I	GCNNGC	1 cut(s)	738
BstDEI	CTNAG	2 cut(s)	367, 741
BstDSI	CCRYGG	1 cut(s)	150
BstF5I	GGATG	1 cut(s)	604
BstFNI	CGCG	1 cut(s)	50
BstHHI	GCGC	1 cut(s)	236
BstKTI	GATC	1 cut(s)	751
BstMAI	GTCTC	1 cut(s)	398
BstMBI	GATC	1 cut(s)	748
BstMWI	GCNNNNNNNGC	5 cut(s)	141, 161, 260, 309, 742
BstNI	CCWGG	2 cut(s)	132, 470
BstNSI	RCATGY	1 cut(s)	234
BstSCI	CCNGG	2 cut(s)	130, 468
BstSFI	CTRYAG	1 cut(s)	618
BstUI	CGCG	1 cut(s)	50
BsuRI	GGCC	3 cut(s)	155, 303, 400
BtgI	CCRYGG	1 cut(s)	150
BtgZI	GCGATG	1 cut(s)	31
BtsCI	GGATG	1 cut(s)	604
BveI	ACCTGC	1 cut(s)	227
Cac8I	GCNNGC	1 cut(s)	738
CfoI	GCGC	1 cut(s)	236
Cfr13I	GGNCC	3 cut(s)	398, 452, 728
CseI	GACGC	1 cut(s)	67
Csp6I	GTAC	3 cut(s)	317, 371, 521
CviAII	CATG	2 cut(s)	151, 231
CviQI	GTAC	3 cut(s)	317, 371, 521
DdeI	CTNAG	2 cut(s)	367, 741
DpnI	GATC	1 cut(s)	750
DpnII	GATC	1 cut(s)	748
EaeI	YGGCCR	1 cut(s)	153
Eam1104I	CTCTTC	1 cut(s)	549
EarI	CTCTTC	1 cut(s)	549
EciI	GGCGGA	1 cut(s)	488
Eco130I	CCWWGG	2 cut(s)	73, 150
Eco32I	GATATC	1 cut(s)	364
Eco47I	GGWCC	2 cut(s)	452, 728
Eco88I	CYCGRG	2 cut(s)	608, 731
EcoO109I	RGGNCCY	1 cut(s)	452
EcoRII	CCWGG	2 cut(s)	130, 468
EcoRV	GATATC	1 cut(s)	364
EcoT14I	CCWWGG	2 cut(s)	73, 150
ErhI	CCWWGG	2 cut(s)	73, 150
FaeI	CATG	2 cut(s)	154, 234
FaiI	YATR	9 cut(s)	152, 174, 232, 245, 321, 409, 425, 647, 758
FalI	AAGNNNNNCTT	2 cut(s)	528, 560
FaqI	GGGAC	3 cut(s)	241, 470, 714
FatI	CATG	2 cut(s)	150, 230
Fnu4HI	GCNGC	4 cut(s)	51, 59, 62, 136
FokI	GGATG	1 cut(s)	591
Fsp4HI	GCNGC	4 cut(s)	51, 59, 62, 136
FspI	TGCGCA	1 cut(s)	235
GlaI	GCGC	1 cut(s)	235
GluI	GCNGC	4 cut(s)	51, 59, 62, 136
GsuI	CTGGAG	1 cut(s)	417
HaeIII	GGCC	3 cut(s)	155, 303, 400
HapII	CCGG	1 cut(s)	185
HgaI	GACGC	1 cut(s)	67
HhaI	GCGC	1 cut(s)	236
Hin1I	GRCGYC	1 cut(s)	78
Hin1II	CATG	2 cut(s)	154, 234
Hin6I	GCGC	1 cut(s)	234
HinP1I	GCGC	1 cut(s)	234
HinfI	GANTC	1 cut(s)	343
HpaII	CCGG	1 cut(s)	185
HphI	GGTGA	1 cut(s)	100
Hpy188I	TCNGA	2 cut(s)	11, 273
Hpy188III	TCNNGA	9 cut(s)	82, 287, 389, 443, 455, 530, 548, 608, 731
Hpy99I	CGWCG	2 cut(s)	11, 83
HpyAV	CCTTC	2 cut(s)	85, 386
HpyCH4III	ACNGT	5 cut(s)	7, 112, 316, 520, 638
HpyCH4IV	ACGT	2 cut(s)	193, 427
HpyCH4V	TGCA	3 cut(s)	25, 312, 620
HpyF10VI	GCNNNNNNNGC	5 cut(s)	141, 161, 260, 309, 742
HpyF3I	CTNAG	2 cut(s)	367, 741
HpySE526I	ACGT	2 cut(s)	193, 427
Hsp92I	GRCGYC	1 cut(s)	78
Hsp92II	CATG	2 cut(s)	154, 234
HspAI	GCGC	1 cut(s)	234
Kzo9I	GATC	1 cut(s)	748
LguI	GCTCTTC	1 cut(s)	549
LmnI	GCTCC	3 cut(s)	436, 518, 674
LweI	GCATC	1 cut(s)	529
MaeII	ACGT	2 cut(s)	193, 427
MalI	GATC	1 cut(s)	750
MboI	GATC	1 cut(s)	748
MboII	GAAGA	6 cut(s)	108, 322, 536, 548, 672, 743
MhlI	GDGCHC	3 cut(s)	45, 146, 433
MlsI	TGGCCA	1 cut(s)	155
MluCI	AATT	2 cut(s)	290, 525
MluNI	TGGCCA	1 cut(s)	155
MmeI	TCCRAC	1 cut(s)	691
MnlI	CCTC	4 cut(s)	271, 275, 384, 526
Mox20I	TGGCCA	1 cut(s)	155
MroXI	GAANNNNTTC	1 cut(s)	329
MscI	TGGCCA	1 cut(s)	155
MseI	TTAA	1 cut(s)	204
MslI	CAYNNNNRTG	2 cut(s)	149, 650
Msp20I	TGGCCA	1 cut(s)	155
MspA1I	CMGCKG	1 cut(s)	437
MspI	CCGG	1 cut(s)	185
MspR9I	CCNGG	2 cut(s)	132, 470
MvaI	CCWGG	2 cut(s)	132, 470
MvnI	CGCG	1 cut(s)	50
MwoI	GCNNNNNNNGC	5 cut(s)	141, 161, 260, 309, 742
NcoI	CCATGG	1 cut(s)	150
NdeII	GATC	1 cut(s)	748
NlaIII	CATG	2 cut(s)	154, 234
NlaIV	GGNNCC	1 cut(s)	730
NmeAIII	GCCGAG	1 cut(s)	190
NsbI	TGCGCA	1 cut(s)	235
NspI	RCATGY	1 cut(s)	234
PciSI	GCTCTTC	1 cut(s)	549
PdmI	GAANNNNTTC	1 cut(s)	329
PfeI	GAWTC	1 cut(s)	343
PkrI	GCNGC	4 cut(s)	52, 60, 63, 137
Ppu21I	YACGTR	2 cut(s)	194, 428
PpuMI	RGGWCCY	1 cut(s)	452
Psp5II	RGGWCCY	1 cut(s)	452
Psp6I	CCWGG	2 cut(s)	130, 468
PspGI	CCWGG	2 cut(s)	130, 468
PspN4I	GGNNCC	1 cut(s)	730
PspPI	GGNCC	3 cut(s)	398, 452, 728
PspPPI	RGGWCCY	1 cut(s)	452
PstI	CTGCAG	1 cut(s)	622
PvuII	CAGCTG	1 cut(s)	437
RsaI	GTAC	3 cut(s)	318, 372, 522
RsaNI	GTAC	3 cut(s)	317, 371, 521
RseI	CAYNNNNRTG	2 cut(s)	149, 650
SapI	GCTCTTC	1 cut(s)	549
SaqAI	TTAA	1 cut(s)	204
SatI	GCNGC	4 cut(s)	51, 59, 62, 136
Sau3AI	GATC	1 cut(s)	748
Sau96I	GGNCC	3 cut(s)	398, 452, 728
ScrFI	CCNGG	2 cut(s)	132, 470
SduI	GDGCHC	3 cut(s)	45, 146, 433
SfaNI	GCATC	1 cut(s)	529
SfcI	CTRYAG	1 cut(s)	618
SinI	GGWCC	2 cut(s)	452, 728
SmiMI	CAYNNNNRTG	2 cut(s)	149, 650
SmlI	CTYRAG	1 cut(s)	387
SmoI	CTYRAG	1 cut(s)	387
Sse9I	AATT	2 cut(s)	290, 525
SsiI	CCGC	5 cut(s)	50, 58, 61, 135, 473
StyD4I	CCNGG	2 cut(s)	130, 468
StyI	CCWWGG	2 cut(s)	73, 150
TaaI	ACNGT	5 cut(s)	7, 112, 316, 520, 638
TaiI	ACGT	2 cut(s)	196, 430
TaqI	TCGA	3 cut(s)	81, 462, 628
TasI	AATT	2 cut(s)	290, 525
TauI	GCSGC	4 cut(s)	53, 61, 64, 138
TfiI	GAWTC	1 cut(s)	343
Tru1I	TTAA	1 cut(s)	204
Tru9I	TTAA	1 cut(s)	204
TspDTI	ATGAA	3 cut(s)	412, 593, 634
VpaK11BI	GGWCC	2 cut(s)	452, 728
XapI	RAATTY	1 cut(s)	525
XceI	RCATGY	1 cut(s)	234
XmnI	GAANNNNTTC	1 cut(s)	329

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.