AT5G61440

Thioredoxin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0010978
Physical Location & Seq
Forward (+)
24707445 .. 24709396
1952 bp
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UTR
Exon/CDS
Intron
AT5G61440.1

Sequence Viewer

Length: 738 bp
ATGGATGCAATTTCATCTCTTGGAACCAATTGTGTTCTTTCTGGGGTTTCTCCATTTTCACAAGAGAATCAATCAAAATCGAAACTTTCTCCGTTTATGAGTCTTGATTTGAAGGAACATCCAATGGCTTCAGCAGATTTCACCAACCAAACCTTAACCGCTTTCTCTTCTTCCTCTGCGTCTCCTTTTCAGGCAAAGACATCATCGATTGGTATGAGTAGAGGAATGAGATGGTGGGAGAAGTCAACAAATCACAACATGTTAGAGATTCAATCTGCCAATCATCTTGTTGACTCTTTGTTAAACGCTGGAGATAGATTGGTTGTTCTTGATTTCTACTCTCCTGGTTGTGGTGGCTGCAAATCTCTCCACCCAAAGATATGTCAATTGGCAGAAACAAACCCAAATGTGATGTTTCTGAAAGTGAATCAAGAAGAGCTTAGAACAATGTGTCATGGTCTAAACGTTCATGTGCTGCCTTTCTTTAAGTTCTATAGAGGAGCAGAGGGTAAAGTCTGTAGCTTCAGCTGCACTATTGCAACTATCAATAAGTTCAAGAAAGCGTTGGATAAGCACGGTAGTGAAAGATGCAGCCTCGGGGATGCAAAGGGATTAGACGAGAAGGAACTCGCGGCTTTGGCATCGGTTGGTGAACTGAAAATGAATTCTTTAACAATGCATCAAGCTAGCAACATTGGTTATAAAACAGAGGAGCAATATCAAACGATGGTGTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Thioredoxin

Protein Analysis

245

Amino Acids

26.89

Weight (kDa)

7.6

Isoelectric Point (pI)

43.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thioredoxin PF00085 98 - 171 4.7e-14 Thioredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010978)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G61440 AT5G61440
fragaria_vesca FvH4_5g14700
malus_domestica MD06G1195500.v1.1 MD14G1202600.v1.1 MD16G1196100.v1.1
prunus_persica Prupe.5G195400_v2.0.a1 Prupe.5G195400_v2.0.a1
pyrus_communis pycom06g17270 pycom14g16750
rosa_chinensis RchiOBHm_Chr7g0181691
rosa_laevigata RLG00000005176
rosa_multiflora Rmu_sc0000988.1_g000011
rosa_roxburghii Rroxscaffold_3G00271800
rosa_rugosa Rorug06G0442800
rosa_samantha Rh7AG045300 Rh7BG044500 Rh7CG046000 Rh7DG044900
rosa_wichuraiana Rw7G003770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 702, 736
AccII CGCG 1 cut(s) 632
AciI CCGC 2 cut(s) 159, 632
AclI AACGTT 1 cut(s) 465
AcsI RAATTY 1 cut(s) 664
AcuI CTGAAG 2 cut(s) 114, 508
AfiI CCNNNNNNNGG 1 cut(s) 350
AflIII ACRYGT 1 cut(s) 258
AgsI TTSAA 3 cut(s) 112, 272, 556
AjnI CCWGG 1 cut(s) 343
AleI CACNNNNGTG 1 cut(s) 579
AluBI AGCT 4 cut(s) 439, 522, 528, 686
AluI AGCT 4 cut(s) 439, 522, 528, 686
Alw26I GTCTC 1 cut(s) 186
Ama87I CYCGRG 1 cut(s) 596
ApeKI GCWGC 4 cut(s) 357, 475, 528, 591
ApoI RAATTY 1 cut(s) 664
AsuHPI GGTGA 2 cut(s) 133, 662
AsuNHI GCTAGC 1 cut(s) 686
AvaI CYCGRG 1 cut(s) 596
BbvI GCAGC 4 cut(s) 344, 462, 515, 603
BccI CCATC 2 cut(s) 225, 721
BciT130I CCWGG 1 cut(s) 345
BcoDI GTCTC 1 cut(s) 186
BfaI CTAG 1 cut(s) 687
BfmI CTRYAG 2 cut(s) 493, 517
BisI GCNGC 5 cut(s) 358, 476, 529, 592, 633
BlsI GCNGC 5 cut(s) 359, 477, 530, 593, 634
Bme1390I CCNGG 1 cut(s) 345
BmeT110I CYCGRG 1 cut(s) 596
BmiI GGNNCC 1 cut(s) 25
BmrFI CCNGG 1 cut(s) 345
BmsI GCATC 4 cut(s) 578, 592, 650, 688
BmtI GCTAGC 1 cut(s) 690
BpmI CTGGAG 1 cut(s) 330
Bsa29I ATCGAT 1 cut(s) 206
BsaJI CCNNGG 1 cut(s) 595
Bsc4I CCNNNNNNNGG 1 cut(s) 350
BseBI CCWGG 1 cut(s) 345
BseCI ATCGAT 1 cut(s) 206
BseDI CCNNGG 1 cut(s) 595
BseGI GGATG 3 cut(s) 10, 118, 607
BseLI CCNNNNNNNGG 1 cut(s) 350
BseRI GAGGAG 2 cut(s) 513, 725
BseXI GCAGC 4 cut(s) 344, 462, 515, 603
BsgI GTGCAG 1 cut(s) 514
Bsh1236I CGCG 1 cut(s) 632
BshVI ATCGAT 1 cut(s) 206
BsiHKCI CYCGRG 1 cut(s) 596
BslI CCNNNNNNNGG 1 cut(s) 350
BsmAI GTCTC 1 cut(s) 186
BsmBI CGTCTC 1 cut(s) 186
BsoBI CYCGRG 1 cut(s) 596
BspACI CCGC 2 cut(s) 159, 632
BspDI ATCGAT 1 cut(s) 206
BspFNI CGCG 1 cut(s) 632
BspLI GGNNCC 1 cut(s) 25
BspOI GCTAGC 1 cut(s) 690
BspQI GCTCTTC 1 cut(s) 429
BssECI CCNNGG 1 cut(s) 595
Bst2UI CCWGG 1 cut(s) 345
Bst4CI ACNGT 1 cut(s) 578
Bst6I CTCTTC 2 cut(s) 172, 429
BstC8I GCNNGC 1 cut(s) 688
BstDEI CTNAG 1 cut(s) 440
BstF5I GGATG 3 cut(s) 10, 118, 607
BstFNI CGCG 1 cut(s) 632
BstMAI GTCTC 1 cut(s) 186
BstMWI GCNNNNNNNGC 2 cut(s) 528, 638
BstNI CCWGG 1 cut(s) 345
BstNSI RCATGY 1 cut(s) 262
BstSCI CCNGG 1 cut(s) 343
BstSFI CTRYAG 2 cut(s) 493, 517
BstUI CGCG 1 cut(s) 632
BstV1I GCAGC 4 cut(s) 344, 462, 515, 603
Bsu15I ATCGAT 1 cut(s) 206
BsuTUI ATCGAT 1 cut(s) 206
BtsCI GGATG 3 cut(s) 10, 118, 607
Cac8I GCNNGC 1 cut(s) 688
ClaI ATCGAT 1 cut(s) 206
CseI GACGC 1 cut(s) 168
CviAII CATG 3 cut(s) 259, 455, 470
CviJI RGCY 8 cut(s) 128, 357, 439, 522, 528, 594, 635, 686
CviKI_1 RGCY 8 cut(s) 128, 357, 439, 522, 528, 594, 635, 686
DdeI CTNAG 1 cut(s) 440
Eam1104I CTCTTC 2 cut(s) 172, 429
EarI CTCTTC 2 cut(s) 172, 429
Eco57I CTGAAG 2 cut(s) 114, 508
Eco88I CYCGRG 1 cut(s) 596
EcoRI GAATTC 1 cut(s) 664
EcoRII CCWGG 1 cut(s) 343
EcoT22I ATGCAT 1 cut(s) 681
Esp3I CGTCTC 1 cut(s) 186
FaeI CATG 3 cut(s) 262, 458, 473
FaiI YATR 9 cut(s) 98, 215, 260, 382, 456, 471, 495, 702, 736
FalI AAGNNNNNCTT 2 cut(s) 423, 455
FatI CATG 3 cut(s) 258, 454, 469
Fnu4HI GCNGC 5 cut(s) 358, 476, 529, 592, 633
FokI GGATG 3 cut(s) 17, 105, 614
Fsp4HI GCNGC 5 cut(s) 358, 476, 529, 592, 633
FspBI CTAG 1 cut(s) 687
GluI GCNGC 5 cut(s) 358, 476, 529, 592, 633
GsuI CTGGAG 1 cut(s) 330
HgaI GACGC 1 cut(s) 168
Hin1II CATG 3 cut(s) 262, 458, 473
HincII GTYRAC 2 cut(s) 246, 292
HindII GTYRAC 2 cut(s) 246, 292
HinfI GANTC 5 cut(s) 67, 100, 268, 293, 427
HphI GGTGA 2 cut(s) 133, 662
Hpy166II GTNNAC 3 cut(s) 246, 292, 653
Hpy188I TCNGA 1 cut(s) 420
Hpy188III TCNNGA 4 cut(s) 104, 329, 431, 556
Hpy8I GTNNAC 3 cut(s) 246, 292, 653
HpyAV CCTTC 2 cut(s) 106, 616
HpyCH4III ACNGT 1 cut(s) 578
HpyCH4IV ACGT 1 cut(s) 465
HpyCH4V TGCA 7 cut(s) 8, 360, 531, 539, 591, 605, 679
HpyF10VI GCNNNNNNNGC 2 cut(s) 528, 638
HpyF3I CTNAG 1 cut(s) 440
HpySE526I ACGT 1 cut(s) 465
Hsp92II CATG 3 cut(s) 262, 458, 473
LguI GCTCTTC 1 cut(s) 429
LmnI GCTCC 2 cut(s) 500, 712
LpnPI CCDG 5 cut(s) 27, 176, 294, 330, 357
Lsp1109I GCAGC 4 cut(s) 344, 462, 515, 603
LweI GCATC 4 cut(s) 578, 592, 650, 688
MaeI CTAG 1 cut(s) 687
MaeII ACGT 1 cut(s) 465
MboII GAAGA 3 cut(s) 159, 162, 446
MfeI CAATTG 2 cut(s) 28, 386
MluCI AATT 4 cut(s) 9, 28, 386, 664
MlyI GAGTC 2 cut(s) 109, 287
MmeI TCCRAC 1 cut(s) 546
MnlI CCTC 6 cut(s) 184, 215, 491, 499, 605, 703
Mph1103I ATGCAT 1 cut(s) 681
MseI TTAA 4 cut(s) 155, 302, 486, 671
MslI CAYNNNNRTG 1 cut(s) 579
MspA1I CMGCKG 1 cut(s) 528
MspR9I CCNGG 1 cut(s) 345
MunI CAATTG 2 cut(s) 28, 386
MvaI CCWGG 1 cut(s) 345
MvnI CGCG 1 cut(s) 632
MwoI GCNNNNNNNGC 2 cut(s) 528, 638
NheI GCTAGC 1 cut(s) 686
NlaIII CATG 3 cut(s) 262, 458, 473
NlaIV GGNNCC 1 cut(s) 25
NsiI ATGCAT 1 cut(s) 681
NspI RCATGY 1 cut(s) 262
OliI CACNNNNGTG 1 cut(s) 579
PciI ACATGT 1 cut(s) 258
PciSI GCTCTTC 1 cut(s) 429
PfeI GAWTC 3 cut(s) 67, 268, 427
PkrI GCNGC 5 cut(s) 359, 477, 530, 593, 634
PleI GAGTC 2 cut(s) 108, 287
PpsI GAGTC 2 cut(s) 108, 287
PscI ACATGT 1 cut(s) 258
PsiI TTATAA 2 cut(s) 702, 736
Psp1406I AACGTT 1 cut(s) 465
Psp6I CCWGG 1 cut(s) 343
PspGI CCWGG 1 cut(s) 343
PspN4I GGNNCC 1 cut(s) 25
PvuII CAGCTG 1 cut(s) 528
RseI CAYNNNNRTG 1 cut(s) 579
SapI GCTCTTC 1 cut(s) 429
SaqAI TTAA 4 cut(s) 155, 302, 486, 671
SatI GCNGC 5 cut(s) 358, 476, 529, 592, 633
SchI GAGTC 2 cut(s) 109, 287
ScrFI CCNGG 1 cut(s) 345
SetI ASST 6 cut(s) 155, 441, 468, 524, 530, 688
SfaNI GCATC 4 cut(s) 578, 592, 650, 688
SfcI CTRYAG 2 cut(s) 493, 517
SmiMI CAYNNNNRTG 1 cut(s) 579
Sse9I AATT 4 cut(s) 9, 28, 386, 664
SsiI CCGC 2 cut(s) 159, 632
SspMI CTAG 1 cut(s) 687
StyD4I CCNGG 1 cut(s) 343
TaaI ACNGT 1 cut(s) 578
TaiI ACGT 1 cut(s) 468
TaqI TCGA 2 cut(s) 80, 206
TasI AATT 4 cut(s) 9, 28, 386, 664
TauI GCSGC 1 cut(s) 635
TfiI GAWTC 3 cut(s) 67, 268, 427
Tru1I TTAA 4 cut(s) 155, 302, 486, 671
Tru9I TTAA 4 cut(s) 155, 302, 486, 671
TseI GCWGC 4 cut(s) 357, 475, 528, 591
TspDTI ATGAA 2 cut(s) 458, 677
TspGWI ACGGA 1 cut(s) 81
XapI RAATTY 1 cut(s) 664
XceI RCATGY 1 cut(s) 262
XspI CTAG 1 cut(s) 687
Zsp2I ATGCAT 1 cut(s) 681
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.