MD14G1202600.v1.1

Thioredoxin-like 1-2, chloroplastic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0010978
Physical Location & Seq
Reverse (-)
29108367 .. 29109344
978 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1202600.v1.1.491

Sequence Viewer

Length: 504 bp
ATGGCCAAGGCATTTGCATCACAGAGTGGTTTCTGCATTTCTTCTGGGTTGGATGAAATTGCAGTTGGCTCCAAATCCCCCAAAGGGGAATTCAGGGTTTCTCCCTCCTTCAGTACCTCACAATTTAAGGATGCAAATTCAAGAGCTTTTCCTTCATTGAGTGTTGATTTCTTGGGAAAACCCATGACTCTCTCAGATCACAAGGGTTTGGAGAATTGTAGGGTCAGAAAACCTAGCAATTTCTCTGTGCATGCACAAGCATCTGTCTGTATAAGCAGAGCTATGAGGTGGTGGGAAAAGACCCTTCACCCCAACATGGTGGAGATCCATTCTGCTCAAGAACTTGTTGATTCCTTGAAAAATGCTGGGGACAGATTGGTTGTAGTTGACTTTTACTCACCTGGCTGTGGAGGCTGCAGAGCTCTCCATCCCAAGACGGACCAACGGCACACTAATCCGCCTCGGAATTATGGCAACTTGATTGATTTATTTGTTAAGCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Thioredoxin

Protein Analysis

168

Amino Acids

18.4

Weight (kDa)

9.22

Isoelectric Point (pI)

32.05

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0010978)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G61440 AT5G61440
fragaria_vesca FvH4_5g14700
malus_domestica MD06G1195500.v1.1 MD14G1202600.v1.1 MD16G1196100.v1.1
prunus_persica Prupe.5G195400_v2.0.a1 Prupe.5G195400_v2.0.a1
pyrus_communis pycom06g17270 pycom14g16750
rosa_chinensis RchiOBHm_Chr7g0181691
rosa_laevigata RLG00000005176
rosa_multiflora Rmu_sc0000988.1_g000011
rosa_roxburghii Rroxscaffold_3G00271800
rosa_rugosa Rorug06G0442800
rosa_samantha Rh7AG045300 Rh7BG044500 Rh7CG046000 Rh7DG044900
rosa_wichuraiana Rw7G003770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 458
AclWI GGATC 1 cut(s) 319
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 2 cut(s) 89, 136
AcuI CTGAAG 1 cut(s) 94
AdeI CACNNNGTG 1 cut(s) 26
AfaI GTAC 1 cut(s) 115
AfiI CCNNNNNNNGG 4 cut(s) 84, 85, 316, 407
AgsI TTSAA 2 cut(s) 141, 358
AjnI CCWGG 1 cut(s) 400
AjuI GAANNNNNNNTTGG 4 cut(s) 48, 74, 80, 106
AluBI AGCT 4 cut(s) 146, 281, 422, 499
AluI AGCT 4 cut(s) 146, 281, 422, 499
Alw21I GWGCWC 1 cut(s) 424
AlwI GGATC 1 cut(s) 319
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 1 cut(s) 414
ApoI RAATTY 2 cut(s) 89, 136
AspS9I GGNCC 1 cut(s) 439
AsuHPI GGTGA 2 cut(s) 299, 390
AvaII GGWCC 1 cut(s) 439
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 1 cut(s) 424
Bbv12I GWGCWC 1 cut(s) 424
BbvI GCAGC 1 cut(s) 401
BccI CCATC 1 cut(s) 435
BceAI ACGGC 1 cut(s) 461
BciT130I CCWGG 1 cut(s) 402
BfaI CTAG 1 cut(s) 234
BfmI CTRYAG 1 cut(s) 415
BisI GCNGC 1 cut(s) 415
BlsI GCNGC 1 cut(s) 416
Bme1390I CCNGG 1 cut(s) 402
Bme18I GGWCC 1 cut(s) 439
BmgT120I GGNCC 1 cut(s) 439
BmiI GGNNCC 1 cut(s) 70
BmrFI CCNGG 1 cut(s) 402
BmsI GCATC 3 cut(s) 26, 121, 269
BpuEI CTTGAG 1 cut(s) 321
BsaJI CCNNGG 2 cut(s) 6, 461
Bsc4I CCNNNNNNNGG 4 cut(s) 84, 85, 316, 407
BseBI CCWGG 1 cut(s) 402
BseDI CCNNGG 2 cut(s) 6, 461
BseGI GGATG 3 cut(s) 58, 136, 427
BseLI CCNNNNNNNGG 4 cut(s) 84, 85, 316, 407
BseMII CTCAG 1 cut(s) 207
BseXI GCAGC 1 cut(s) 401
BseYI CCCAGC 1 cut(s) 365
BshFI GGCC 1 cut(s) 5
BsiHKAI GWGCWC 1 cut(s) 424
BslFI GGGAC 1 cut(s) 383
BslI CCNNNNNNNGG 4 cut(s) 84, 85, 316, 407
BsmFI GGGAC 1 cut(s) 383
BsnI GGCC 1 cut(s) 5
Bsp1286I GDGCHC 1 cut(s) 424
Bsp143I GATC 2 cut(s) 196, 324
BspACI CCGC 1 cut(s) 458
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 1 cut(s) 206
BspLI GGNNCC 1 cut(s) 70
BspMAI CTGCAG 1 cut(s) 419
BspPI GGATC 1 cut(s) 319
BssECI CCNNGG 2 cut(s) 6, 461
BssMI GATC 2 cut(s) 196, 324
BssT1I CCWWGG 1 cut(s) 6
Bst2UI CCWGG 1 cut(s) 402
BstC8I GCNNGC 1 cut(s) 252
BstDEI CTNAG 1 cut(s) 193
BstF5I GGATG 3 cut(s) 58, 136, 427
BstKTI GATC 2 cut(s) 199, 327
BstMBI GATC 2 cut(s) 196, 324
BstMWI GCNNNNNNNGC 1 cut(s) 411
BstNI CCWGG 1 cut(s) 402
BstNSI RCATGY 1 cut(s) 254
BstSCI CCNGG 1 cut(s) 400
BstSFI CTRYAG 1 cut(s) 415
BstV1I GCAGC 1 cut(s) 401
BstX2I RGATCY 1 cut(s) 324
BstXI CCANNNNNNTGG 1 cut(s) 319
BstYI RGATCY 1 cut(s) 324
BsuRI GGCC 1 cut(s) 5
BtsCI GGATG 3 cut(s) 58, 136, 427
Cac8I GCNNGC 1 cut(s) 252
Cfr13I GGNCC 1 cut(s) 439
Csp6I GTAC 1 cut(s) 114
CviAII CATG 3 cut(s) 184, 251, 316
CviJI RGCY 8 cut(s) 5, 69, 146, 281, 405, 414, 422, 499
CviKI_1 RGCY 8 cut(s) 5, 69, 146, 281, 405, 414, 422, 499
CviQI GTAC 1 cut(s) 114
DdeI CTNAG 1 cut(s) 193
DpnI GATC 2 cut(s) 198, 326
DpnII GATC 2 cut(s) 196, 324
DraIII CACNNNGTG 1 cut(s) 26
EaeI YGGCCR 1 cut(s) 3
EciI GGCGGA 1 cut(s) 447
Ecl136II GAGCTC 1 cut(s) 422
Eco130I CCWWGG 1 cut(s) 6
Eco24I GRGCYC 1 cut(s) 424
Eco47I GGWCC 1 cut(s) 439
Eco53kI GAGCTC 1 cut(s) 422
Eco57I CTGAAG 1 cut(s) 94
EcoICRI GAGCTC 1 cut(s) 422
EcoRI GAATTC 1 cut(s) 89
EcoRII CCWGG 1 cut(s) 400
EcoT14I CCWWGG 1 cut(s) 6
EcoT38I GRGCYC 1 cut(s) 424
ErhI CCWWGG 1 cut(s) 6
FaeI CATG 3 cut(s) 187, 254, 319
FaiI YATR 6 cut(s) 185, 252, 272, 284, 317, 471
FaqI GGGAC 1 cut(s) 383
FatI CATG 3 cut(s) 183, 250, 315
Fnu4HI GCNGC 1 cut(s) 415
FokI GGATG 3 cut(s) 65, 143, 414
FriOI GRGCYC 1 cut(s) 424
Fsp4HI GCNGC 1 cut(s) 415
FspBI CTAG 1 cut(s) 234
GluI GCNGC 1 cut(s) 415
GsaI CCCAGC 1 cut(s) 369
HaeIII GGCC 1 cut(s) 5
Hin1II CATG 3 cut(s) 187, 254, 319
HincII GTYRAC 1 cut(s) 388
HindII GTYRAC 1 cut(s) 388
HindIII AAGCTT 1 cut(s) 497
HinfI GANTC 2 cut(s) 187, 350
HphI GGTGA 2 cut(s) 299, 390
Hpy166II GTNNAC 1 cut(s) 388
Hpy188I TCNGA 3 cut(s) 196, 227, 465
Hpy188III TCNNGA 2 cut(s) 141, 338
Hpy8I GTNNAC 1 cut(s) 388
HpyAV CCTTC 3 cut(s) 118, 162, 314
HpyCH4V TGCA 7 cut(s) 17, 36, 62, 134, 250, 254, 417
HpyF10VI GCNNNNNNNGC 1 cut(s) 411
HpyF3I CTNAG 1 cut(s) 193
Hsp92II CATG 3 cut(s) 187, 254, 319
Kzo9I GATC 2 cut(s) 196, 324
LmnI GCTCC 1 cut(s) 74
LpnPI CCDG 5 cut(s) 30, 79, 351, 387, 414
Lsp1109I GCAGC 1 cut(s) 401
LweI GCATC 3 cut(s) 26, 121, 269
MaeI CTAG 1 cut(s) 234
MalI GATC 2 cut(s) 198, 326
MboI GATC 2 cut(s) 196, 324
MboII GAAGA 1 cut(s) 33
MflI RGATCY 1 cut(s) 324
MhlI GDGCHC 1 cut(s) 424
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 7 cut(s) 57, 89, 122, 136, 214, 238, 466
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 181
MmeI TCCRAC 1 cut(s) 30
MnlI CCTC 5 cut(s) 115, 127, 279, 404, 471
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 3 cut(s) 126, 495, 502
Msp20I TGGCCA 1 cut(s) 5
MspR9I CCNGG 1 cut(s) 402
MvaI CCWGG 1 cut(s) 402
MwoI GCNNNNNNNGC 1 cut(s) 411
NdeII GATC 2 cut(s) 196, 324
NlaIII CATG 3 cut(s) 187, 254, 319
NlaIV GGNNCC 1 cut(s) 70
NspI RCATGY 1 cut(s) 254
PaeI GCATGC 1 cut(s) 254
PfeI GAWTC 1 cut(s) 350
PkrI GCNGC 1 cut(s) 416
PleI GAGTC 1 cut(s) 181
PpsI GAGTC 1 cut(s) 181
Psp124BI GAGCTC 1 cut(s) 424
Psp6I CCWGG 1 cut(s) 400
PspFI CCCAGC 1 cut(s) 365
PspGI CCWGG 1 cut(s) 400
PspN4I GGNNCC 1 cut(s) 70
PspPI GGNCC 1 cut(s) 439
PstI CTGCAG 1 cut(s) 419
PsuI RGATCY 1 cut(s) 324
RsaI GTAC 1 cut(s) 115
RsaNI GTAC 1 cut(s) 114
SacI GAGCTC 1 cut(s) 424
SaqAI TTAA 3 cut(s) 126, 495, 502
SatI GCNGC 1 cut(s) 415
Sau3AI GATC 2 cut(s) 196, 324
Sau96I GGNCC 1 cut(s) 439
SchI GAGTC 1 cut(s) 181
ScrFI CCNGG 1 cut(s) 402
SduI GDGCHC 1 cut(s) 424
SetI ASST 8 cut(s) 119, 148, 235, 283, 290, 403, 424, 501
SfaNI GCATC 3 cut(s) 26, 121, 269
SfcI CTRYAG 1 cut(s) 415
SinI GGWCC 1 cut(s) 439
SmlI CTYRAG 1 cut(s) 336
SmoI CTYRAG 1 cut(s) 336
SphI GCATGC 1 cut(s) 254
Sse9I AATT 7 cut(s) 57, 89, 122, 136, 214, 238, 466
SsiI CCGC 1 cut(s) 458
SspMI CTAG 1 cut(s) 234
SstI GAGCTC 1 cut(s) 424
StyD4I CCNGG 1 cut(s) 400
StyI CCWWGG 1 cut(s) 6
TasI AATT 7 cut(s) 57, 89, 122, 136, 214, 238, 466
TfiI GAWTC 1 cut(s) 350
Tru1I TTAA 3 cut(s) 126, 495, 502
Tru9I TTAA 3 cut(s) 126, 495, 502
TseI GCWGC 1 cut(s) 414
TspDTI ATGAA 2 cut(s) 69, 144
TspGWI ACGGA 1 cut(s) 452
VpaK11BI GGWCC 1 cut(s) 439
XapI RAATTY 2 cut(s) 89, 136
XceI RCATGY 1 cut(s) 254
XspI CTAG 1 cut(s) 234
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.