FvH4_5g14700

Thioredoxin-like 1-2, chloroplastic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0010978
Physical Location & Seq
Forward (+)
8346188 .. 8348341
2154 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g14700.t1

Sequence Viewer

Length: 804 bp
ATGGCAATTGCTTCAAAGTGTGGTTTGTACATTTCTGGGGTTCATGAGATTAGTCTGCGCTCCAAATCCAGAGAATCAAATTCCAGAGCTTTTCCGGTTTTGGGTGTTGAATTTTTGGGAAAACCCATCTCAGATTACAAGGGCTTAAAGAATTTGAGGGCCAAGGCCCCCAGAAATTTATCTGTTCATGCTCAAGCATCAATCTGTGTAAGCAAAGCTATGAAATGGTGGGAGAAAACCCTAAAACCCAACATGGTGGAGATCCATTCTGCTCAAGAACTCATTGATTCTTTGCATACTGCGGGTGATAGATTGGTTGTAGTGGATTTTTACTCACCTGGTTGTGGAGGTTGCAGAGCTCTCCATCCCAAGATCTGTCAGCTGGCTGAACAGAATCCAGATGCAATTTTTCTCAAGGTTAACTATGAAGAGCTCAAGACTATGTGCCATGGTCTCCACATTCATGTGCTTCCATTCTTTAGGTTTTATAGAGGCGCTGAAGGGCGAGTGTGTAGCTTCAGCTGCACCAATGCCACTATCAAGAAATTCAAAGATGCATTAGCTAAATATGGGGCTGAGGGTTGTAGTCTTGGCCCTGCAAAAGGTTTAGACGAGTCTGAGATATTACACTTGGCTGAGATAGGTGAGATATCAGCAAGATCTTCTCCACCATCAACCAAAGAAGAGAGAGTGGAGGAGGATTTGATCATGAAAAGTATAGATTTGTCTGGTGTTTGGAGTAATGTTATCCATAACCATGACATAGAACTCGCAGAAGAAGAAAGTGCCTTGCTAAAGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Thioredoxin

Protein Analysis

268

Amino Acids

29.49

Weight (kDa)

7.15

Isoelectric Point (pI)

44.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thioredoxin PF00085 102 - 169 1.1e-12 Thioredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010978)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G61440 AT5G61440
fragaria_vesca FvH4_5g14700
malus_domestica MD06G1195500.v1.1 MD14G1202600.v1.1 MD16G1196100.v1.1
prunus_persica Prupe.5G195400_v2.0.a1 Prupe.5G195400_v2.0.a1
pyrus_communis pycom06g17270 pycom14g16750
rosa_chinensis RchiOBHm_Chr7g0181691
rosa_laevigata RLG00000005176
rosa_multiflora Rmu_sc0000988.1_g000011
rosa_roxburghii Rroxscaffold_3G00271800
rosa_rugosa Rorug06G0442800
rosa_samantha Rh7AG045300 Rh7BG044500 Rh7CG046000 Rh7DG044900
rosa_wichuraiana Rw7G003770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 302
AclWI GGATC 1 cut(s) 256
AcsI RAATTY 5 cut(s) 79, 110, 151, 175, 545
AcuI CTGAAG 2 cut(s) 502, 519
AfaI GTAC 1 cut(s) 29
AfiI CCNNNNNNNGG 3 cut(s) 101, 344, 602
AgsI TTSAA 3 cut(s) 15, 110, 550
AjnI CCWGG 1 cut(s) 337
AluBI AGCT 8 cut(s) 89, 218, 359, 382, 433, 516, 522, 563
AluI AGCT 8 cut(s) 89, 218, 359, 382, 433, 516, 522, 563
Alw21I GWGCWC 2 cut(s) 361, 435
Alw26I GTCTC 1 cut(s) 458
AlwI GGATC 1 cut(s) 256
AoxI GGCC 3 cut(s) 159, 165, 592
ApeKI GCWGC 1 cut(s) 522
ApoI RAATTY 5 cut(s) 79, 110, 151, 175, 545
AspLEI GCGC 2 cut(s) 60, 497
AspS9I GGNCC 3 cut(s) 159, 166, 593
AsuHPI GGTGA 3 cut(s) 317, 327, 656
BanII GRGCYC 2 cut(s) 361, 435
Bbv12I GWGCWC 2 cut(s) 361, 435
BbvCI CCTCAGC 1 cut(s) 576
BbvI GCAGC 1 cut(s) 509
BccI CCATC 3 cut(s) 134, 372, 679
BciT130I CCWGG 1 cut(s) 339
BclI TGATCA 1 cut(s) 705
BcoDI GTCTC 1 cut(s) 458
BfoI RGCGCY 1 cut(s) 498
BglII AGATCT 2 cut(s) 372, 659
BisI GCNGC 1 cut(s) 523
BlsI GCNGC 1 cut(s) 524
Bme1390I CCNGG 1 cut(s) 339
BmgT120I GGNCC 3 cut(s) 159, 166, 593
BmiI GGNNCC 1 cut(s) 168
BmrFI CCNGG 1 cut(s) 339
BmsI GCATC 3 cut(s) 206, 391, 544
Bpu10I CCTNAGC 1 cut(s) 576
BpuEI CTTGAG 4 cut(s) 177, 258, 398, 419
BsaI GGTCTC 1 cut(s) 458
BsaJI CCNNGG 2 cut(s) 162, 448
BsaWI WCCGGW 1 cut(s) 94
Bsc4I CCNNNNNNNGG 3 cut(s) 101, 344, 602
BseBI CCWGG 1 cut(s) 339
BseDI CCNNGG 2 cut(s) 162, 448
BseGI GGATG 1 cut(s) 364
BseLI CCNNNNNNNGG 3 cut(s) 101, 344, 602
BseMII CTCAG 4 cut(s) 144, 567, 609, 627
BseRI GAGGAG 1 cut(s) 710
BseXI GCAGC 1 cut(s) 509
BsgI GTGCAG 1 cut(s) 508
BshFI GGCC 3 cut(s) 161, 167, 594
BsiHKAI GWGCWC 2 cut(s) 361, 435
BsiSI CCGG 1 cut(s) 95
BslI CCNNNNNNNGG 3 cut(s) 101, 344, 602
BsmAI GTCTC 1 cut(s) 458
BsnI GGCC 3 cut(s) 161, 167, 594
Bso31I GGTCTC 1 cut(s) 458
Bsp1286I GDGCHC 2 cut(s) 361, 435
Bsp1407I TGTACA 1 cut(s) 27
Bsp143I GATC 4 cut(s) 261, 372, 659, 705
Bsp19I CCATGG 1 cut(s) 448
BspACI CCGC 1 cut(s) 302
BspANI GGCC 3 cut(s) 161, 167, 594
BspCNI CTCAG 4 cut(s) 143, 568, 610, 628
BspHI TCATGA 2 cut(s) 43, 708
BspLI GGNNCC 1 cut(s) 168
BspPI GGATC 1 cut(s) 256
BspQI GCTCTTC 1 cut(s) 423
BspTNI GGTCTC 1 cut(s) 458
BsrGI TGTACA 1 cut(s) 27
BssECI CCNNGG 2 cut(s) 162, 448
BssMI GATC 4 cut(s) 261, 372, 659, 705
BssT1I CCWWGG 2 cut(s) 162, 448
Bst2UI CCWGG 1 cut(s) 339
Bst6I CTCTTC 2 cut(s) 423, 678
BstAUI TGTACA 1 cut(s) 27
BstC8I GCNNGC 1 cut(s) 384
BstDEI CTNAG 4 cut(s) 130, 576, 618, 636
BstDSI CCRYGG 1 cut(s) 448
BstENI CCTNNNNNAGG 1 cut(s) 600
BstF5I GGATG 1 cut(s) 364
BstH2I RGCGCY 1 cut(s) 498
BstHHI GCGC 2 cut(s) 60, 497
BstKTI GATC 4 cut(s) 264, 375, 662, 708
BstMAI GTCTC 1 cut(s) 458
BstMBI GATC 4 cut(s) 261, 372, 659, 705
BstMWI GCNNNNNNNGC 1 cut(s) 522
BstNI CCWGG 1 cut(s) 339
BstSCI CCNGG 1 cut(s) 337
BstV1I GCAGC 1 cut(s) 509
BstX2I RGATCY 3 cut(s) 261, 372, 659
BstXI CCANNNNNNTGG 1 cut(s) 256
BstYI RGATCY 3 cut(s) 261, 372, 659
BsuRI GGCC 3 cut(s) 161, 167, 594
BtgI CCRYGG 1 cut(s) 448
BtsCI GGATG 1 cut(s) 364
Cac8I GCNNGC 1 cut(s) 384
CciI TCATGA 2 cut(s) 43, 708
CfoI GCGC 2 cut(s) 60, 497
Cfr13I GGNCC 3 cut(s) 159, 166, 593
CsiI ACCWGGT 1 cut(s) 337
Csp6I GTAC 1 cut(s) 28
CviAII CATG 7 cut(s) 44, 188, 253, 449, 464, 709, 758
CviQI GTAC 1 cut(s) 28
DdeI CTNAG 4 cut(s) 130, 576, 618, 636
DpnI GATC 4 cut(s) 263, 374, 661, 707
DpnII GATC 4 cut(s) 261, 372, 659, 705
Eam1104I CTCTTC 2 cut(s) 423, 678
EarI CTCTTC 2 cut(s) 423, 678
Ecl136II GAGCTC 2 cut(s) 359, 433
Eco130I CCWWGG 2 cut(s) 162, 448
Eco24I GRGCYC 2 cut(s) 361, 435
Eco31I GGTCTC 1 cut(s) 458
Eco32I GATATC 1 cut(s) 651
Eco53kI GAGCTC 2 cut(s) 359, 433
Eco57I CTGAAG 2 cut(s) 502, 519
EcoICRI GAGCTC 2 cut(s) 359, 433
EcoNI CCTNNNNNAGG 1 cut(s) 600
EcoO109I RGGNCCY 1 cut(s) 166
EcoRII CCWGG 1 cut(s) 337
EcoRV GATATC 1 cut(s) 651
EcoT14I CCWWGG 2 cut(s) 162, 448
EcoT22I ATGCAT 1 cut(s) 559
EcoT38I GRGCYC 2 cut(s) 361, 435
ErhI CCWWGG 2 cut(s) 162, 448
FaeI CATG 7 cut(s) 47, 191, 256, 452, 467, 712, 761
FatI CATG 7 cut(s) 43, 187, 252, 448, 463, 708, 757
FauI CCCGC 1 cut(s) 295
FbaI TGATCA 1 cut(s) 705
Fnu4HI GCNGC 1 cut(s) 523
FokI GGATG 1 cut(s) 351
FriOI GRGCYC 2 cut(s) 361, 435
Fsp4HI GCNGC 1 cut(s) 523
GlaI GCGC 2 cut(s) 59, 496
GluI GCNGC 1 cut(s) 523
HaeII RGCGCY 1 cut(s) 498
HaeIII GGCC 3 cut(s) 161, 167, 594
HapII CCGG 1 cut(s) 95
HhaI GCGC 2 cut(s) 60, 497
Hin1II CATG 7 cut(s) 47, 191, 256, 452, 467, 712, 761
Hin6I GCGC 2 cut(s) 58, 495
HinP1I GCGC 2 cut(s) 58, 495
HincII GTYRAC 1 cut(s) 421
HindII GTYRAC 1 cut(s) 421
HinfI GANTC 4 cut(s) 74, 287, 394, 614
HpaI GTTAAC 1 cut(s) 421
HpaII CCGG 1 cut(s) 95
HphI GGTGA 3 cut(s) 317, 327, 656
Hpy166II GTNNAC 1 cut(s) 421
Hpy188I TCNGA 2 cut(s) 133, 619
Hpy188III TCNNGA 8 cut(s) 44, 69, 84, 275, 398, 436, 541, 709
Hpy8I GTNNAC 1 cut(s) 421
HpyAV CCTTC 1 cut(s) 494
HpyCH4V TGCA 6 cut(s) 295, 354, 404, 525, 557, 599
HpyF10VI GCNNNNNNNGC 1 cut(s) 522
HpyF3I CTNAG 4 cut(s) 130, 576, 618, 636
Hsp92II CATG 7 cut(s) 47, 191, 256, 452, 467, 712, 761
HspAI GCGC 2 cut(s) 58, 495
Ksp22I TGATCA 1 cut(s) 705
KspAI GTTAAC 1 cut(s) 421
Kzo9I GATC 4 cut(s) 261, 372, 659, 705
LguI GCTCTTC 1 cut(s) 423
LmnI GCTCC 1 cut(s) 65
Lsp1109I GCAGC 1 cut(s) 509
LweI GCATC 3 cut(s) 206, 391, 544
MabI ACCWGGT 1 cut(s) 337
MalI GATC 4 cut(s) 263, 374, 661, 707
MboI GATC 4 cut(s) 261, 372, 659, 705
MboII GAAGA 5 cut(s) 440, 654, 695, 788, 791
MfeI CAATTG 1 cut(s) 6
MflI RGATCY 3 cut(s) 261, 372, 659
MhlI GDGCHC 2 cut(s) 361, 435
MluCI AATT 7 cut(s) 6, 79, 110, 151, 175, 405, 545
MlyI GAGTC 1 cut(s) 623
MnlI CCTC 6 cut(s) 150, 341, 485, 571, 688, 691
Mph1103I ATGCAT 1 cut(s) 559
MseI TTAA 2 cut(s) 146, 420
MslI CAYNNNNRTG 3 cut(s) 462, 464, 756
MspA1I CMGCKG 2 cut(s) 382, 522
MspI CCGG 1 cut(s) 95
MspR9I CCNGG 1 cut(s) 339
MunI CAATTG 1 cut(s) 6
MvaI CCWGG 1 cut(s) 339
MwoI GCNNNNNNNGC 1 cut(s) 522
NcoI CCATGG 1 cut(s) 448
NdeII GATC 4 cut(s) 261, 372, 659, 705
NlaIII CATG 7 cut(s) 47, 191, 256, 452, 467, 712, 761
NlaIV GGNNCC 1 cut(s) 168
NsiI ATGCAT 1 cut(s) 559
PagI TCATGA 2 cut(s) 43, 708
PciSI GCTCTTC 1 cut(s) 423
PfeI GAWTC 3 cut(s) 74, 287, 394
PkrI GCNGC 1 cut(s) 524
PleI GAGTC 1 cut(s) 622
PpsI GAGTC 1 cut(s) 622
Psp124BI GAGCTC 2 cut(s) 361, 435
Psp6I CCWGG 1 cut(s) 337
PspGI CCWGG 1 cut(s) 337
PspN4I GGNNCC 1 cut(s) 168
PspPI GGNCC 3 cut(s) 159, 166, 593
PsuI RGATCY 3 cut(s) 261, 372, 659
PvuII CAGCTG 2 cut(s) 382, 522
RsaI GTAC 1 cut(s) 29
RsaNI GTAC 1 cut(s) 28
RseI CAYNNNNRTG 3 cut(s) 462, 464, 756
SacI GAGCTC 2 cut(s) 361, 435
SapI GCTCTTC 1 cut(s) 423
SaqAI TTAA 2 cut(s) 146, 420
SatI GCNGC 1 cut(s) 523
Sau3AI GATC 4 cut(s) 261, 372, 659, 705
Sau96I GGNCC 3 cut(s) 159, 166, 593
SchI GAGTC 1 cut(s) 623
ScrFI CCNGG 1 cut(s) 339
SduI GDGCHC 2 cut(s) 361, 435
SexAI ACCWGGT 1 cut(s) 337
SfaNI GCATC 3 cut(s) 206, 391, 544
SmiMI CAYNNNNRTG 3 cut(s) 462, 464, 756
SmlI CTYRAG 4 cut(s) 192, 273, 413, 434
SmoI CTYRAG 4 cut(s) 192, 273, 413, 434
Sse9I AATT 7 cut(s) 6, 79, 110, 151, 175, 405, 545
SsiI CCGC 1 cut(s) 302
SstI GAGCTC 2 cut(s) 361, 435
StyD4I CCNGG 1 cut(s) 337
StyI CCWWGG 2 cut(s) 162, 448
TasI AATT 7 cut(s) 6, 79, 110, 151, 175, 405, 545
TatI WGTACW 1 cut(s) 27
TfiI GAWTC 3 cut(s) 74, 287, 394
Tru1I TTAA 2 cut(s) 146, 420
Tru9I TTAA 2 cut(s) 146, 420
TseI GCWGC 1 cut(s) 522
TspDTI ATGAA 6 cut(s) 32, 176, 236, 441, 452, 725
XagI CCTNNNNNAGG 1 cut(s) 600
XapI RAATTY 5 cut(s) 79, 110, 151, 175, 545
Zsp2I ATGCAT 1 cut(s) 559
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.