pycom06g17270

Thioredoxin-like 1-2, chloroplastic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0010978
Physical Location & Seq
Reverse (-)
21275907 .. 21277178
1272 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g17270.4

Sequence Viewer

Length: 843 bp
ATGGCCAATGCATTTGCATCACAGACTGGTTTCTGTATTTCTTCTGGGTTGGATGAAATTGCAGCTGGCTCCAAATCCCCCAAAGGGGATTTTAGGGTTTCTCCTACCTCCCAATTTAAGGATGCAAATTCAAGAGCTTTTCCTGCATTGAGTGTTGATTTCTTGGGAAAACCCTTGATTCTCTCAGATCAGGGTTTCGAGAATTGGAGAGTCAGAAAACCAAGCAATTTCTCTGTGCATGCAAAAGCATCTCTGTGTGTAAGCAGAGCAATGAGATGGTGGGAGAAGACCCTTCACCCCAACATGGTGGAGATCCATTCTGCTCAAGAACTTGTTGATTCTTTGAAGAATGCTGGAGACAGATTGGTTGTGGTTGACTTTTACTCACCTGGTTGTGGAGGCTGCAGAGCTCTCCATCCCAAGATCTGTCAGCTTGCTGAATCGAACCCGAATGCGATTTTCCTCAAAGTTAACTATGAAGAGCACAAGACTATGTGTCATGCTCTCAACATTCATGTGCTGCCGTTCTTTAGATTCTTTAGAGGTGCAGATGGTCGTGTGTGTAGCTTTAGCTGCACCAATGCAACTATTAAGAAATTCAAAGATGCATTGGCAAAACACGGGTCTGATCGTTGTAATCTTGGGCCGGCGAAAGGGTTAGATGAATCTGAGATAATAAAGTTAGCTTCAATAGGTGAAATATTAACAACAACATCATCGCCTTCCACGAAGGAAGAAAGATTGGAGGATCTGGTCACGAAAAGCGTAGATTTTTCAGGTGTGTGGAGCAATGAAAGCCATAGCATAGAACTTGAGGAAGAAGGTTCCATGGTGAAGGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Thioredoxin

Protein Analysis

281

Amino Acids

30.78

Weight (kDa)

7.1

Isoelectric Point (pI)

36.9

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010978)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G61440 AT5G61440
fragaria_vesca FvH4_5g14700
malus_domestica MD06G1195500.v1.1 MD14G1202600.v1.1 MD16G1196100.v1.1
prunus_persica Prupe.5G195400_v2.0.a1 Prupe.5G195400_v2.0.a1
pyrus_communis pycom06g17270 pycom14g16750
rosa_chinensis RchiOBHm_Chr7g0181691
rosa_laevigata RLG00000005176
rosa_multiflora Rmu_sc0000988.1_g000011
rosa_roxburghii Rroxscaffold_3G00271800
rosa_rugosa Rorug06G0442800
rosa_samantha Rh7AG045300 Rh7BG044500 Rh7CG046000 Rh7DG044900
rosa_wichuraiana Rw7G003770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 307, 756
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 2 cut(s) 127, 596
AfiI CCNNNNNNNGG 6 cut(s) 84, 85, 118, 304, 395, 653
AgsI TTSAA 4 cut(s) 132, 346, 601, 690
AhdI GACNNNNNGTC 1 cut(s) 495
AjnI CCWGG 1 cut(s) 388
AluBI AGCT 7 cut(s) 65, 137, 410, 433, 567, 573, 686
AluI AGCT 7 cut(s) 65, 137, 410, 433, 567, 573, 686
Alw21I GWGCWC 2 cut(s) 412, 486
Alw26I GTCTC 1 cut(s) 351
AlwI GGATC 2 cut(s) 307, 756
AoxI GGCC 2 cut(s) 3, 644
ApeKI GCWGC 4 cut(s) 62, 402, 520, 573
ApoI RAATTY 2 cut(s) 127, 596
ArsI GACNNNNNNTTYG 2 cut(s) 608, 640
AspS9I GGNCC 1 cut(s) 644
AsuHPI GGTGA 3 cut(s) 287, 378, 707
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 1 cut(s) 412
BbsI GAAGAC 1 cut(s) 293
Bbv12I GWGCWC 2 cut(s) 412, 486
BbvI GCAGC 4 cut(s) 74, 389, 507, 560
BccI CCATC 3 cut(s) 270, 423, 545
BceAI ACGGC 1 cut(s) 508
BciT130I CCWGG 1 cut(s) 390
BcoDI GTCTC 1 cut(s) 351
BfmI CTRYAG 1 cut(s) 403
BglII AGATCT 1 cut(s) 423
BisI GCNGC 4 cut(s) 63, 403, 521, 574
BlsI GCNGC 4 cut(s) 64, 404, 522, 575
Bme1390I CCNGG 1 cut(s) 390
BmeRI GACNNNNNGTC 1 cut(s) 495
BmgT120I GGNCC 1 cut(s) 644
BmiI GGNNCC 2 cut(s) 70, 826
BmrFI CCNGG 1 cut(s) 390
BmsI GCATC 4 cut(s) 26, 112, 257, 595
BpiI GAAGAC 1 cut(s) 293
BpmI CTGGAG 1 cut(s) 375
BpuEI CTTGAG 2 cut(s) 309, 833
BsaJI CCNNGG 1 cut(s) 828
Bsc4I CCNNNNNNNGG 6 cut(s) 84, 85, 118, 304, 395, 653
Bse118I RCCGGY 1 cut(s) 646
Bse1I ACTGG 1 cut(s) 31
Bse3DI GCAATG 2 cut(s) 276, 796
BseBI CCWGG 1 cut(s) 390
BseDI CCNNGG 1 cut(s) 828
BseGI GGATG 3 cut(s) 58, 127, 415
BseLI CCNNNNNNNGG 6 cut(s) 84, 85, 118, 304, 395, 653
BseMI GCAATG 2 cut(s) 276, 796
BseMII CTCAG 2 cut(s) 198, 660
BseNI ACTGG 1 cut(s) 31
BseXI GCAGC 4 cut(s) 74, 389, 507, 560
BsgI GTGCAG 2 cut(s) 559, 567
BshFI GGCC 2 cut(s) 5, 646
BsiHKAI GWGCWC 2 cut(s) 412, 486
BsiSI CCGG 1 cut(s) 647
BslI CCNNNNNNNGG 6 cut(s) 84, 85, 118, 304, 395, 653
BsmAI GTCTC 1 cut(s) 351
BsmI GAATGC 2 cut(s) 355, 457
BsnI GGCC 2 cut(s) 5, 646
Bsp1286I GDGCHC 2 cut(s) 412, 486
Bsp143I GATC 5 cut(s) 187, 312, 423, 628, 748
Bsp19I CCATGG 1 cut(s) 828
BspANI GGCC 2 cut(s) 5, 646
BspCNI CTCAG 2 cut(s) 197, 661
BspLI GGNNCC 2 cut(s) 70, 826
BspMAI CTGCAG 1 cut(s) 407
BspPI GGATC 2 cut(s) 307, 756
BspQI GCTCTTC 1 cut(s) 474
BsrDI GCAATG 2 cut(s) 276, 796
BsrFI RCCGGY 1 cut(s) 646
BsrI ACTGG 1 cut(s) 31
BssAI RCCGGY 1 cut(s) 646
BssECI CCNNGG 1 cut(s) 828
BssMI GATC 5 cut(s) 187, 312, 423, 628, 748
BssT1I CCWWGG 1 cut(s) 828
Bst2UI CCWGG 1 cut(s) 390
Bst6I CTCTTC 1 cut(s) 474
BstC8I GCNNGC 4 cut(s) 67, 240, 435, 648
BstDEI CTNAG 2 cut(s) 184, 669
BstDSI CCRYGG 1 cut(s) 828
BstF5I GGATG 3 cut(s) 58, 127, 415
BstKTI GATC 5 cut(s) 190, 315, 426, 631, 751
BstMAI GTCTC 1 cut(s) 351
BstMBI GATC 5 cut(s) 187, 312, 423, 628, 748
BstMWI GCNNNNNNNGC 3 cut(s) 143, 573, 795
BstNI CCWGG 1 cut(s) 390
BstNSI RCATGY 1 cut(s) 242
BstSCI CCNGG 1 cut(s) 388
BstSFI CTRYAG 1 cut(s) 403
BstV1I GCAGC 4 cut(s) 74, 389, 507, 560
BstV2I GAAGAC 1 cut(s) 293
BstX2I RGATCY 3 cut(s) 312, 423, 748
BstXI CCANNNNNNTGG 1 cut(s) 307
BstYI RGATCY 3 cut(s) 312, 423, 748
BsuRI GGCC 2 cut(s) 5, 646
BtgI CCRYGG 1 cut(s) 828
BtgZI GCGATG 1 cut(s) 702
BtsCI GGATG 3 cut(s) 58, 127, 415
Cac8I GCNNGC 4 cut(s) 67, 240, 435, 648
Cfr10I RCCGGY 1 cut(s) 646
Cfr13I GGNCC 1 cut(s) 644
CsiI ACCWGGT 1 cut(s) 388
CviAII CATG 5 cut(s) 239, 304, 500, 515, 829
DdeI CTNAG 2 cut(s) 184, 669
DpnI GATC 5 cut(s) 189, 314, 425, 630, 750
DpnII GATC 5 cut(s) 187, 312, 423, 628, 748
DriI GACNNNNNGTC 1 cut(s) 495
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 474
Eam1105I GACNNNNNGTC 1 cut(s) 495
EarI CTCTTC 1 cut(s) 474
Ecl136II GAGCTC 1 cut(s) 410
Eco130I CCWWGG 1 cut(s) 828
Eco24I GRGCYC 1 cut(s) 412
Eco53kI GAGCTC 1 cut(s) 410
EcoICRI GAGCTC 1 cut(s) 410
EcoRII CCWGG 1 cut(s) 388
EcoT14I CCWWGG 1 cut(s) 828
EcoT22I ATGCAT 2 cut(s) 13, 610
EcoT38I GRGCYC 1 cut(s) 412
ErhI CCWWGG 1 cut(s) 828
FaeI CATG 5 cut(s) 242, 307, 503, 518, 832
FaiI YATR 9 cut(s) 240, 305, 477, 494, 501, 516, 801, 806, 830
FatI CATG 5 cut(s) 238, 303, 499, 514, 828
Fnu4HI GCNGC 4 cut(s) 63, 403, 521, 574
FokI GGATG 3 cut(s) 65, 134, 402
FriOI GRGCYC 1 cut(s) 412
Fsp4HI GCNGC 4 cut(s) 63, 403, 521, 574
GluI GCNGC 4 cut(s) 63, 403, 521, 574
GsuI CTGGAG 1 cut(s) 375
HaeIII GGCC 2 cut(s) 5, 646
HapII CCGG 1 cut(s) 647
Hin1II CATG 5 cut(s) 242, 307, 503, 518, 832
HincII GTYRAC 2 cut(s) 376, 472
HindII GTYRAC 2 cut(s) 376, 472
HinfI GANTC 6 cut(s) 178, 210, 338, 440, 534, 665
HpaI GTTAAC 1 cut(s) 472
HpaII CCGG 1 cut(s) 647
HphI GGTGA 3 cut(s) 287, 378, 707
Hpy166II GTNNAC 2 cut(s) 376, 472
Hpy188I TCNGA 4 cut(s) 187, 215, 628, 670
Hpy188III TCNNGA 4 cut(s) 132, 199, 326, 757
Hpy8I GTNNAC 2 cut(s) 376, 472
HpyAV CCTTC 5 cut(s) 302, 724, 732, 815, 829
HpyF10VI GCNNNNNNNGC 3 cut(s) 143, 573, 795
HpyF3I CTNAG 2 cut(s) 184, 669
Hsp92II CATG 5 cut(s) 242, 307, 503, 518, 832
KroI GCCGGC 1 cut(s) 646
KroNI GCCGGC 1 cut(s) 648
KspAI GTTAAC 1 cut(s) 472
Kzo9I GATC 5 cut(s) 187, 312, 423, 628, 748
LguI GCTCTTC 1 cut(s) 474
LmnI GCTCC 2 cut(s) 74, 786
Lsp1109I GCAGC 4 cut(s) 74, 389, 507, 560
LweI GCATC 4 cut(s) 26, 112, 257, 595
MabI ACCWGGT 1 cut(s) 388
MaeIII GTNAC 1 cut(s) 754
MalI GATC 5 cut(s) 189, 314, 425, 630, 750
MboI GATC 5 cut(s) 187, 312, 423, 628, 748
MboII GAAGA 6 cut(s) 33, 298, 358, 491, 746, 830
MflI RGATCY 3 cut(s) 312, 423, 748
MhlI GDGCHC 2 cut(s) 412, 486
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 6 cut(s) 57, 113, 127, 202, 226, 596
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 219
MmeI TCCRAC 1 cut(s) 30
MnlI CCTC 6 cut(s) 118, 392, 473, 536, 739, 808
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 2 cut(s) 13, 610
MroNI GCCGGC 1 cut(s) 646
MscI TGGCCA 1 cut(s) 5
MseI TTAA 5 cut(s) 117, 471, 591, 704, 841
MslI CAYNNNNRTG 2 cut(s) 253, 515
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 65
MspI CCGG 1 cut(s) 647
MspR9I CCNGG 1 cut(s) 390
Mva1269I GAATGC 2 cut(s) 355, 457
MvaI CCWGG 1 cut(s) 390
MwoI GCNNNNNNNGC 3 cut(s) 143, 573, 795
NaeI GCCGGC 1 cut(s) 648
NcoI CCATGG 1 cut(s) 828
NdeII GATC 5 cut(s) 187, 312, 423, 628, 748
NgoMIV GCCGGC 1 cut(s) 646
NlaIII CATG 5 cut(s) 242, 307, 503, 518, 832
NlaIV GGNNCC 2 cut(s) 70, 826
NmuCI GTSAC 1 cut(s) 754
NsiI ATGCAT 2 cut(s) 13, 610
NspI RCATGY 1 cut(s) 242
PaeI GCATGC 1 cut(s) 242
PciSI GCTCTTC 1 cut(s) 474
PcsI WCGNNNNNNNCGW 1 cut(s) 725
PctI GAATGC 2 cut(s) 355, 457
PdiI GCCGGC 1 cut(s) 648
PfeI GAWTC 5 cut(s) 178, 338, 440, 534, 665
PkrI GCNGC 4 cut(s) 64, 404, 522, 575
PleI GAGTC 1 cut(s) 218
PpsI GAGTC 1 cut(s) 218
Psp124BI GAGCTC 1 cut(s) 412
Psp6I CCWGG 1 cut(s) 388
PspGI CCWGG 1 cut(s) 388
PspN4I GGNNCC 2 cut(s) 70, 826
PspPI GGNCC 1 cut(s) 644
PstI CTGCAG 1 cut(s) 407
PsuI RGATCY 3 cut(s) 312, 423, 748
PvuII CAGCTG 1 cut(s) 65
RseI CAYNNNNRTG 2 cut(s) 253, 515
SacI GAGCTC 1 cut(s) 412
SapI GCTCTTC 1 cut(s) 474
SaqAI TTAA 5 cut(s) 117, 471, 591, 704, 841
SatI GCNGC 4 cut(s) 63, 403, 521, 574
Sau3AI GATC 5 cut(s) 187, 312, 423, 628, 748
Sau96I GGNCC 1 cut(s) 644
SchI GAGTC 1 cut(s) 219
ScrFI CCNGG 1 cut(s) 390
SduI GDGCHC 2 cut(s) 412, 486
SexAI ACCWGGT 1 cut(s) 388
SfaNI GCATC 4 cut(s) 26, 112, 257, 595
SfcI CTRYAG 1 cut(s) 403
SmiMI CAYNNNNRTG 2 cut(s) 253, 515
SmlI CTYRAG 2 cut(s) 324, 812
SmoI CTYRAG 2 cut(s) 324, 812
SphI GCATGC 1 cut(s) 242
Sse9I AATT 6 cut(s) 57, 113, 127, 202, 226, 596
SspI AATATT 1 cut(s) 702
SstI GAGCTC 1 cut(s) 412
StyD4I CCNGG 1 cut(s) 388
StyI CCWWGG 1 cut(s) 828
TaqI TCGA 2 cut(s) 198, 443
TasI AATT 6 cut(s) 57, 113, 127, 202, 226, 596
TfiI GAWTC 5 cut(s) 178, 338, 440, 534, 665
Tru1I TTAA 5 cut(s) 117, 471, 591, 704, 841
Tru9I TTAA 5 cut(s) 117, 471, 591, 704, 841
TseFI GTSAC 1 cut(s) 754
TseI GCWGC 4 cut(s) 62, 402, 520, 573
Tsp45I GTSAC 1 cut(s) 754
TspDTI ATGAA 5 cut(s) 69, 492, 503, 678, 807
XapI RAATTY 2 cut(s) 127, 596
XceI RCATGY 1 cut(s) 242
Zsp2I ATGCAT 2 cut(s) 13, 610
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.