Rh7AG045300

Thioredoxin-like 1-2, chloroplastic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0010978
Physical Location & Seq
Reverse (-)
3068597 .. 3071111
2515 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG045300.1

Sequence Viewer

Length: 582 bp
ATGGCAATTTCATCAAAGTGTGGTTTGTACATTTCTGGGGTAAATGAGATGAGTCTGCGCTCCAAATTCACAGAATCAAATTCAAGAGCTTTTCCTGTTTTGGGTGTTGTTTTTTGGGGAAAACCCATCTCAGATCACAAGGGTTTGAAGAATTTGAGGGTCAAGGCCCCGAGAAATTTCTCTGTTCATGCACAAGCATCAATCTGTGTAAGCAAAGCTATGAAATGGTGGGAGAAAACCCTAAAACCCAACATGGTGGAGATCCATTCTGCTCAAGAACTCATTGATTCTTTGCATAATGCTGGTGATAGATTGGTTGTAGTGGATTTTTACTCACCTGGTTGTGGAGGTTGTAGAGCTCTCCATCCCAAGATCTGTCAGCTGGCTGAACAGAACCCAGATGCAATTTTTCTTAAGGTTAACTATGAGAAGCTCAAGACTATGTGCCATGGTCTCCACATTCATGTGCTACCATTCTTTAGGTTTTATAGAGGCGCAGAAGGGCGAGTTTGTAGCTTCAGCTGCACCAATGCCACTGTAAGTCTTGCAATCCTGTCAATATTGGTTTGTAATATTCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Thioredoxin

Protein Analysis

193

Amino Acids

21.51

Weight (kDa)

9.39

Isoelectric Point (pI)

39.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thioredoxin PF00085 98 - 167 6.3e-13 Thioredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010978)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G61440 AT5G61440
fragaria_vesca FvH4_5g14700
malus_domestica MD06G1195500.v1.1 MD14G1202600.v1.1 MD16G1196100.v1.1
prunus_persica Prupe.5G195400_v2.0.a1 Prupe.5G195400_v2.0.a1
pyrus_communis pycom06g17270 pycom14g16750
rosa_chinensis RchiOBHm_Chr7g0181691
rosa_laevigata RLG00000005176
rosa_multiflora Rmu_sc0000988.1_g000011
rosa_roxburghii Rroxscaffold_3G00271800
rosa_rugosa Rorug06G0442800
rosa_samantha Rh7AG045300 Rh7BG044500 Rh7CG046000 Rh7DG044900
rosa_wichuraiana Rw7G003770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 256
AcsI RAATTY 4 cut(s) 65, 79, 151, 175
AcuI CTGAAG 1 cut(s) 502
AfaI GTAC 1 cut(s) 29
AfiI CCNNNNNNNGG 2 cut(s) 101, 344
AflII CTTAAG 1 cut(s) 413
AgsI TTSAA 2 cut(s) 84, 148
AjnI CCWGG 1 cut(s) 337
AluBI AGCT 7 cut(s) 89, 218, 359, 382, 433, 516, 522
AluI AGCT 7 cut(s) 89, 218, 359, 382, 433, 516, 522
Alw21I GWGCWC 1 cut(s) 361
Alw26I GTCTC 1 cut(s) 458
AlwI GGATC 1 cut(s) 256
Ama87I CYCGRG 1 cut(s) 169
AoxI GGCC 1 cut(s) 165
ApeKI GCWGC 1 cut(s) 522
ApoI RAATTY 4 cut(s) 65, 79, 151, 175
AspLEI GCGC 2 cut(s) 60, 497
AspS9I GGNCC 1 cut(s) 166
AsuHPI GGTGA 2 cut(s) 317, 327
AvaI CYCGRG 1 cut(s) 169
BanII GRGCYC 1 cut(s) 361
Bbv12I GWGCWC 1 cut(s) 361
BbvI GCAGC 1 cut(s) 509
BccI CCATC 2 cut(s) 134, 372
BciT130I CCWGG 1 cut(s) 339
BcoDI GTCTC 1 cut(s) 458
BfrI CTTAAG 1 cut(s) 413
BglII AGATCT 1 cut(s) 372
BisI GCNGC 1 cut(s) 523
BlsI GCNGC 1 cut(s) 524
Bme1390I CCNGG 1 cut(s) 339
BmeT110I CYCGRG 1 cut(s) 169
BmgT120I GGNCC 1 cut(s) 166
BmiI GGNNCC 1 cut(s) 168
BmrFI CCNGG 1 cut(s) 339
BmsI GCATC 2 cut(s) 206, 391
BpuEI CTTGAG 2 cut(s) 258, 419
BsaI GGTCTC 1 cut(s) 458
BsaJI CCNNGG 1 cut(s) 448
Bsc4I CCNNNNNNNGG 2 cut(s) 101, 344
BseBI CCWGG 1 cut(s) 339
BseDI CCNNGG 1 cut(s) 448
BseGI GGATG 1 cut(s) 364
BseLI CCNNNNNNNGG 2 cut(s) 101, 344
BseMII CTCAG 1 cut(s) 144
BseXI GCAGC 1 cut(s) 509
BsgI GTGCAG 1 cut(s) 508
BshFI GGCC 1 cut(s) 167
BsiHKAI GWGCWC 1 cut(s) 361
BsiHKCI CYCGRG 1 cut(s) 169
BslI CCNNNNNNNGG 2 cut(s) 101, 344
BsmAI GTCTC 1 cut(s) 458
BsnI GGCC 1 cut(s) 167
Bso31I GGTCTC 1 cut(s) 458
BsoBI CYCGRG 1 cut(s) 169
Bsp1286I GDGCHC 1 cut(s) 361
Bsp1407I TGTACA 1 cut(s) 27
Bsp143I GATC 3 cut(s) 133, 261, 372
Bsp19I CCATGG 1 cut(s) 448
BspANI GGCC 1 cut(s) 167
BspCNI CTCAG 1 cut(s) 143
BspLI GGNNCC 1 cut(s) 168
BspPI GGATC 1 cut(s) 256
BspTI CTTAAG 1 cut(s) 413
BspTNI GGTCTC 1 cut(s) 458
BsrGI TGTACA 1 cut(s) 27
BssECI CCNNGG 1 cut(s) 448
BssMI GATC 3 cut(s) 133, 261, 372
BssT1I CCWWGG 1 cut(s) 448
Bst2UI CCWGG 1 cut(s) 339
Bst4CI ACNGT 1 cut(s) 538
BstAFI CTTAAG 1 cut(s) 413
BstAUI TGTACA 1 cut(s) 27
BstC8I GCNNGC 1 cut(s) 384
BstDEI CTNAG 1 cut(s) 130
BstDSI CCRYGG 1 cut(s) 448
BstF5I GGATG 1 cut(s) 364
BstHHI GCGC 2 cut(s) 60, 497
BstKTI GATC 3 cut(s) 136, 264, 375
BstMAI GTCTC 1 cut(s) 458
BstMBI GATC 3 cut(s) 133, 261, 372
BstMWI GCNNNNNNNGC 1 cut(s) 522
BstNI CCWGG 1 cut(s) 339
BstSCI CCNGG 1 cut(s) 337
BstV1I GCAGC 1 cut(s) 509
BstX2I RGATCY 2 cut(s) 261, 372
BstXI CCANNNNNNTGG 1 cut(s) 256
BstYI RGATCY 2 cut(s) 261, 372
BsuRI GGCC 1 cut(s) 167
BtgI CCRYGG 1 cut(s) 448
BtsCI GGATG 1 cut(s) 364
BtsIMutI CAGTG 1 cut(s) 534
Cac8I GCNNGC 1 cut(s) 384
CfoI GCGC 2 cut(s) 60, 497
Cfr13I GGNCC 1 cut(s) 166
CsiI ACCWGGT 1 cut(s) 337
Csp6I GTAC 1 cut(s) 28
CviAII CATG 4 cut(s) 188, 253, 449, 464
CviJI RGCY 9 cut(s) 89, 167, 218, 359, 382, 386, 433, 516, 522
CviKI_1 RGCY 9 cut(s) 89, 167, 218, 359, 382, 386, 433, 516, 522
CviQI GTAC 1 cut(s) 28
DdeI CTNAG 1 cut(s) 130
DpnI GATC 3 cut(s) 135, 263, 374
DpnII GATC 3 cut(s) 133, 261, 372
Ecl136II GAGCTC 1 cut(s) 359
Eco130I CCWWGG 1 cut(s) 448
Eco24I GRGCYC 1 cut(s) 361
Eco31I GGTCTC 1 cut(s) 458
Eco53kI GAGCTC 1 cut(s) 359
Eco57I CTGAAG 1 cut(s) 502
Eco88I CYCGRG 1 cut(s) 169
EcoICRI GAGCTC 1 cut(s) 359
EcoO109I RGGNCCY 1 cut(s) 166
EcoRII CCWGG 1 cut(s) 337
EcoT14I CCWWGG 1 cut(s) 448
EcoT38I GRGCYC 1 cut(s) 361
ErhI CCWWGG 1 cut(s) 448
FaeI CATG 4 cut(s) 191, 256, 452, 467
FaiI YATR 9 cut(s) 189, 221, 254, 297, 426, 443, 450, 465, 489
FatI CATG 4 cut(s) 187, 252, 448, 463
Fnu4HI GCNGC 1 cut(s) 523
FokI GGATG 1 cut(s) 351
FriOI GRGCYC 1 cut(s) 361
Fsp4HI GCNGC 1 cut(s) 523
GlaI GCGC 2 cut(s) 59, 496
GluI GCNGC 1 cut(s) 523
HaeIII GGCC 1 cut(s) 167
HhaI GCGC 2 cut(s) 60, 497
Hin1II CATG 4 cut(s) 191, 256, 452, 467
Hin6I GCGC 2 cut(s) 58, 495
HinP1I GCGC 2 cut(s) 58, 495
HincII GTYRAC 1 cut(s) 421
HindII GTYRAC 1 cut(s) 421
HinfI GANTC 3 cut(s) 52, 74, 287
HpaI GTTAAC 1 cut(s) 421
HphI GGTGA 2 cut(s) 317, 327
Hpy166II GTNNAC 1 cut(s) 421
Hpy188I TCNGA 1 cut(s) 133
Hpy188III TCNNGA 3 cut(s) 84, 275, 436
Hpy8I GTNNAC 1 cut(s) 421
HpyAV CCTTC 1 cut(s) 494
HpyCH4III ACNGT 1 cut(s) 538
HpyCH4V TGCA 5 cut(s) 191, 295, 404, 525, 548
HpyF10VI GCNNNNNNNGC 1 cut(s) 522
HpyF3I CTNAG 1 cut(s) 130
Hsp92II CATG 4 cut(s) 191, 256, 452, 467
HspAI GCGC 2 cut(s) 58, 495
KspAI GTTAAC 1 cut(s) 421
Kzo9I GATC 3 cut(s) 133, 261, 372
LmnI GCTCC 1 cut(s) 65
LpnPI CCDG 8 cut(s) 21, 108, 288, 324, 351, 368, 411, 566
Lsp1109I GCAGC 1 cut(s) 509
LweI GCATC 2 cut(s) 206, 391
MabI ACCWGGT 1 cut(s) 337
MalI GATC 3 cut(s) 135, 263, 374
MboI GATC 3 cut(s) 133, 261, 372
MboII GAAGA 1 cut(s) 160
MflI RGATCY 2 cut(s) 261, 372
MhlI GDGCHC 1 cut(s) 361
MluCI AATT 6 cut(s) 6, 65, 79, 151, 175, 405
MlyI GAGTC 1 cut(s) 61
MnlI CCTC 3 cut(s) 150, 341, 485
MseI TTAA 2 cut(s) 414, 420
MslI CAYNNNNRTG 3 cut(s) 16, 462, 464
MspA1I CMGCKG 2 cut(s) 382, 522
MspCI CTTAAG 1 cut(s) 413
MspR9I CCNGG 1 cut(s) 339
MvaI CCWGG 1 cut(s) 339
MwoI GCNNNNNNNGC 1 cut(s) 522
NcoI CCATGG 1 cut(s) 448
NdeII GATC 3 cut(s) 133, 261, 372
NlaIII CATG 4 cut(s) 191, 256, 452, 467
NlaIV GGNNCC 1 cut(s) 168
PfeI GAWTC 2 cut(s) 74, 287
PkrI GCNGC 1 cut(s) 524
PleI GAGTC 1 cut(s) 60
PpsI GAGTC 1 cut(s) 60
Psp124BI GAGCTC 1 cut(s) 361
Psp6I CCWGG 1 cut(s) 337
PspGI CCWGG 1 cut(s) 337
PspN4I GGNNCC 1 cut(s) 168
PspPI GGNCC 1 cut(s) 166
PsuI RGATCY 2 cut(s) 261, 372
PvuII CAGCTG 2 cut(s) 382, 522
RsaI GTAC 1 cut(s) 29
RsaNI GTAC 1 cut(s) 28
RseI CAYNNNNRTG 3 cut(s) 16, 462, 464
SacI GAGCTC 1 cut(s) 361
SaqAI TTAA 2 cut(s) 414, 420
SatI GCNGC 1 cut(s) 523
Sau3AI GATC 3 cut(s) 133, 261, 372
Sau96I GGNCC 1 cut(s) 166
SchI GAGTC 1 cut(s) 61
ScrFI CCNGG 1 cut(s) 339
SduI GDGCHC 1 cut(s) 361
SexAI ACCWGGT 1 cut(s) 337
SfaNI GCATC 2 cut(s) 206, 391
SmiMI CAYNNNNRTG 3 cut(s) 16, 462, 464
SmlI CTYRAG 3 cut(s) 273, 413, 434
SmoI CTYRAG 3 cut(s) 273, 413, 434
Sse9I AATT 6 cut(s) 6, 65, 79, 151, 175, 405
SspI AATATT 2 cut(s) 561, 574
SstI GAGCTC 1 cut(s) 361
StyD4I CCNGG 1 cut(s) 337
StyI CCWWGG 1 cut(s) 448
TaaI ACNGT 1 cut(s) 538
TaqI TCGA 1 cut(s) 577
TasI AATT 6 cut(s) 6, 65, 79, 151, 175, 405
TatI WGTACW 1 cut(s) 27
TfiI GAWTC 2 cut(s) 74, 287
Tru1I TTAA 2 cut(s) 414, 420
Tru9I TTAA 2 cut(s) 414, 420
TscAI CASTG 1 cut(s) 541
TseI GCWGC 1 cut(s) 522
TspDTI ATGAA 3 cut(s) 176, 236, 452
TspRI CASTG 1 cut(s) 541
Vha464I CTTAAG 1 cut(s) 413
XapI RAATTY 4 cut(s) 65, 79, 151, 175
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.