Rroxscaffold_3G00271800

Thioredoxin-like 1-2, chloroplastic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0010978
Physical Location & Seq
Forward (+)
64135946 .. 64137594
1649 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00271800.1

Sequence Viewer

Length: 804 bp
ATGGCAATTTCATCAAAGTGTGGTTTGTACGTTTCTGGGGTAAATGAGATGAGTCTGCGCTCCAAATTCACAGAATCAAATTCAAGAGCTTTTCCTGTTTTGGGTGTTGTTTTTTGGGGAAAACCCATCTCAGATCACAAGGGTTTGAAAAATTTGAGGGTCAAGGCCCCGAGAAATTTCTCTGTTCATGCACAAGCTTCAATCTGTGTAAGCAAAGCCATGAAATGGTGGGAGAAAACCCTAAAACCCAACATGGTGGAGATCCATTCTGCTCAAGAACTCATTGATTCTTTGCATAATGGTGGTGATAGATTGGTTGTAGTGGATTTTTACTCACCTGGTTGTGGAGGTTGTAGAGCTCTCCATCCCAAGATCTGTCAGCTGGCTGAACAGAACCCAGATGCAATTTTTCTTAAGGTTAACTATGAGGAGCTCAAGACTGTGTGCCATGGTCTCCACATTCATGTGCTACCATTCTTTAGGTTTTATAGAGGCGCAGAAGGGCGAGTTTGTAGCTTCAGCTGCACCAATGCCACTATCAAGAAATTCAAAGATGCATTAGCTAAATATGGGGCTGAGGGTTGTAGTCTTGGCCCAGCAAAAGGCTTAGATGAGTCTGAGATATTACACTTAGCTGAGATAGGTGAAATATCAGCAAGATCTTCTCCACCATCGACCAAAGAAGAGAGAGTGGAGGAGGATTTGGTCATGAAAAGCATAGATTTGTCTGGTGTTTGGAGCAATGTAATCCATAACCATGGCGTAGAACTTGAAGAAGAAGAAAGTGCCTTGCTGAAGGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Thioredoxin

Protein Analysis

267

Amino Acids

29.5

Weight (kDa)

7.15

Isoelectric Point (pI)

45.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thioredoxin PF00085 98 - 169 8.1e-13 Thioredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010978)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G61440 AT5G61440
fragaria_vesca FvH4_5g14700
malus_domestica MD06G1195500.v1.1 MD14G1202600.v1.1 MD16G1196100.v1.1
prunus_persica Prupe.5G195400_v2.0.a1 Prupe.5G195400_v2.0.a1
pyrus_communis pycom06g17270 pycom14g16750
rosa_chinensis RchiOBHm_Chr7g0181691
rosa_laevigata RLG00000005176
rosa_multiflora Rmu_sc0000988.1_g000011
rosa_roxburghii Rroxscaffold_3G00271800
rosa_rugosa Rorug06G0442800
rosa_samantha Rh7AG045300 Rh7BG044500 Rh7CG046000 Rh7DG044900
rosa_wichuraiana Rw7G003770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 225
AclWI GGATC 1 cut(s) 256
AcsI RAATTY 5 cut(s) 65, 79, 151, 175, 545
AcuI CTGAAG 1 cut(s) 502
AfaI GTAC 1 cut(s) 29
AfiI CCNNNNNNNGG 4 cut(s) 101, 225, 344, 602
AflII CTTAAG 1 cut(s) 413
AgsI TTSAA 5 cut(s) 84, 148, 201, 550, 773
AjnI CCWGG 1 cut(s) 337
AluBI AGCT 9 cut(s) 89, 197, 359, 382, 433, 516, 522, 563, 635
AluI AGCT 9 cut(s) 89, 197, 359, 382, 433, 516, 522, 563, 635
Alw21I GWGCWC 2 cut(s) 361, 435
Alw26I GTCTC 1 cut(s) 458
AlwI GGATC 1 cut(s) 256
Ama87I CYCGRG 1 cut(s) 169
AoxI GGCC 2 cut(s) 165, 592
ApeKI GCWGC 1 cut(s) 522
ApoI RAATTY 5 cut(s) 65, 79, 151, 175, 545
AspLEI GCGC 2 cut(s) 60, 497
AspS9I GGNCC 2 cut(s) 166, 593
AsuHPI GGTGA 3 cut(s) 317, 327, 656
AvaI CYCGRG 1 cut(s) 169
BanII GRGCYC 2 cut(s) 361, 435
Bbv12I GWGCWC 2 cut(s) 361, 435
BbvCI CCTCAGC 1 cut(s) 576
BbvI GCAGC 1 cut(s) 509
BccI CCATC 3 cut(s) 134, 372, 679
BciT130I CCWGG 1 cut(s) 339
BcoDI GTCTC 1 cut(s) 458
BfrI CTTAAG 1 cut(s) 413
BglII AGATCT 2 cut(s) 372, 659
BisI GCNGC 1 cut(s) 523
BlsI GCNGC 1 cut(s) 524
Bme1390I CCNGG 1 cut(s) 339
BmeT110I CYCGRG 1 cut(s) 169
BmgT120I GGNCC 2 cut(s) 166, 593
BmiI GGNNCC 1 cut(s) 168
BmrFI CCNGG 1 cut(s) 339
BmsI GCATC 2 cut(s) 391, 544
Bpu10I CCTNAGC 1 cut(s) 576
BpuEI CTTGAG 2 cut(s) 258, 419
BsaI GGTCTC 1 cut(s) 458
BsaJI CCNNGG 2 cut(s) 448, 757
Bsc4I CCNNNNNNNGG 4 cut(s) 101, 225, 344, 602
Bse3DI GCAATG 1 cut(s) 748
BseBI CCWGG 1 cut(s) 339
BseDI CCNNGG 2 cut(s) 448, 757
BseGI GGATG 1 cut(s) 364
BseLI CCNNNNNNNGG 4 cut(s) 101, 225, 344, 602
BseMI GCAATG 1 cut(s) 748
BseMII CTCAG 4 cut(s) 144, 567, 609, 627
BseRI GAGGAG 2 cut(s) 443, 710
BseXI GCAGC 1 cut(s) 509
BseYI CCCAGC 1 cut(s) 595
BsgI GTGCAG 1 cut(s) 508
BshFI GGCC 2 cut(s) 167, 594
BsiHKAI GWGCWC 2 cut(s) 361, 435
BsiHKCI CYCGRG 1 cut(s) 169
BslI CCNNNNNNNGG 4 cut(s) 101, 225, 344, 602
BsmAI GTCTC 1 cut(s) 458
BsnI GGCC 2 cut(s) 167, 594
Bso31I GGTCTC 1 cut(s) 458
BsoBI CYCGRG 1 cut(s) 169
Bsp1286I GDGCHC 2 cut(s) 361, 435
Bsp143I GATC 4 cut(s) 133, 261, 372, 659
Bsp19I CCATGG 2 cut(s) 448, 757
BspANI GGCC 2 cut(s) 167, 594
BspCNI CTCAG 4 cut(s) 143, 568, 610, 628
BspHI TCATGA 1 cut(s) 708
BspLI GGNNCC 1 cut(s) 168
BspPI GGATC 1 cut(s) 256
BspTI CTTAAG 1 cut(s) 413
BspTNI GGTCTC 1 cut(s) 458
BsrDI GCAATG 1 cut(s) 748
BssECI CCNNGG 2 cut(s) 448, 757
BssMI GATC 4 cut(s) 133, 261, 372, 659
BssT1I CCWWGG 2 cut(s) 448, 757
Bst2UI CCWGG 1 cut(s) 339
Bst4CI ACNGT 1 cut(s) 442
Bst6I CTCTTC 1 cut(s) 678
BstAFI CTTAAG 1 cut(s) 413
BstC8I GCNNGC 1 cut(s) 384
BstDEI CTNAG 6 cut(s) 130, 576, 607, 618, 631, 636
BstDSI CCRYGG 2 cut(s) 448, 757
BstF5I GGATG 1 cut(s) 364
BstHHI GCGC 2 cut(s) 60, 497
BstKTI GATC 4 cut(s) 136, 264, 375, 662
BstMAI GTCTC 1 cut(s) 458
BstMBI GATC 4 cut(s) 133, 261, 372, 659
BstMWI GCNNNNNNNGC 1 cut(s) 522
BstNI CCWGG 1 cut(s) 339
BstSCI CCNGG 1 cut(s) 337
BstV1I GCAGC 1 cut(s) 509
BstX2I RGATCY 3 cut(s) 261, 372, 659
BstXI CCANNNNNNTGG 2 cut(s) 256, 758
BstYI RGATCY 3 cut(s) 261, 372, 659
BsuRI GGCC 2 cut(s) 167, 594
BtgI CCRYGG 2 cut(s) 448, 757
BtsCI GGATG 1 cut(s) 364
Cac8I GCNNGC 1 cut(s) 384
CciI TCATGA 1 cut(s) 708
CfoI GCGC 2 cut(s) 60, 497
Cfr13I GGNCC 2 cut(s) 166, 593
CsiI ACCWGGT 1 cut(s) 337
Csp6I GTAC 1 cut(s) 28
CviAII CATG 7 cut(s) 188, 220, 253, 449, 464, 709, 758
CviQI GTAC 1 cut(s) 28
DdeI CTNAG 6 cut(s) 130, 576, 607, 618, 631, 636
DpnI GATC 4 cut(s) 135, 263, 374, 661
DpnII GATC 4 cut(s) 133, 261, 372, 659
Eam1104I CTCTTC 1 cut(s) 678
EarI CTCTTC 1 cut(s) 678
Ecl136II GAGCTC 2 cut(s) 359, 433
Eco130I CCWWGG 2 cut(s) 448, 757
Eco24I GRGCYC 2 cut(s) 361, 435
Eco31I GGTCTC 1 cut(s) 458
Eco53kI GAGCTC 2 cut(s) 359, 433
Eco57I CTGAAG 1 cut(s) 502
Eco88I CYCGRG 1 cut(s) 169
EcoICRI GAGCTC 2 cut(s) 359, 433
EcoO109I RGGNCCY 1 cut(s) 166
EcoRII CCWGG 1 cut(s) 337
EcoT14I CCWWGG 2 cut(s) 448, 757
EcoT22I ATGCAT 1 cut(s) 559
EcoT38I GRGCYC 2 cut(s) 361, 435
ErhI CCWWGG 2 cut(s) 448, 757
FaeI CATG 7 cut(s) 191, 223, 256, 452, 467, 712, 761
FatI CATG 7 cut(s) 187, 219, 252, 448, 463, 708, 757
Fnu4HI GCNGC 1 cut(s) 523
FokI GGATG 1 cut(s) 351
FriOI GRGCYC 2 cut(s) 361, 435
Fsp4HI GCNGC 1 cut(s) 523
GlaI GCGC 2 cut(s) 59, 496
GluI GCNGC 1 cut(s) 523
GsaI CCCAGC 1 cut(s) 599
HaeIII GGCC 2 cut(s) 167, 594
HhaI GCGC 2 cut(s) 60, 497
Hin1II CATG 7 cut(s) 191, 223, 256, 452, 467, 712, 761
Hin6I GCGC 2 cut(s) 58, 495
HinP1I GCGC 2 cut(s) 58, 495
HincII GTYRAC 1 cut(s) 421
HindII GTYRAC 1 cut(s) 421
HindIII AAGCTT 1 cut(s) 195
HinfI GANTC 4 cut(s) 52, 74, 287, 614
HpaI GTTAAC 1 cut(s) 421
HphI GGTGA 3 cut(s) 317, 327, 656
Hpy166II GTNNAC 1 cut(s) 421
Hpy188I TCNGA 2 cut(s) 133, 619
Hpy188III TCNNGA 5 cut(s) 84, 275, 436, 541, 709
Hpy8I GTNNAC 1 cut(s) 421
HpyAV CCTTC 2 cut(s) 494, 790
HpyCH4III ACNGT 1 cut(s) 442
HpyCH4IV ACGT 1 cut(s) 30
HpyCH4V TGCA 5 cut(s) 191, 295, 404, 525, 557
HpyF10VI GCNNNNNNNGC 1 cut(s) 522
HpyF3I CTNAG 6 cut(s) 130, 576, 607, 618, 631, 636
HpySE526I ACGT 1 cut(s) 30
Hsp92II CATG 7 cut(s) 191, 223, 256, 452, 467, 712, 761
HspAI GCGC 2 cut(s) 58, 495
KspAI GTTAAC 1 cut(s) 421
Kzo9I GATC 4 cut(s) 133, 261, 372, 659
LmnI GCTCC 3 cut(s) 65, 430, 738
LpnPI CCDG 8 cut(s) 21, 108, 324, 351, 368, 411, 609, 714
Lsp1109I GCAGC 1 cut(s) 509
LweI GCATC 2 cut(s) 391, 544
MabI ACCWGGT 1 cut(s) 337
MaeII ACGT 1 cut(s) 30
MalI GATC 4 cut(s) 135, 263, 374, 661
MboI GATC 4 cut(s) 133, 261, 372, 659
MboII GAAGA 5 cut(s) 654, 695, 785, 788, 791
MflI RGATCY 3 cut(s) 261, 372, 659
MhlI GDGCHC 2 cut(s) 361, 435
MluCI AATT 7 cut(s) 6, 65, 79, 151, 175, 405, 545
MlyI GAGTC 2 cut(s) 61, 623
MnlI CCTC 7 cut(s) 150, 341, 421, 485, 571, 688, 691
Mph1103I ATGCAT 1 cut(s) 559
MseI TTAA 2 cut(s) 414, 420
MslI CAYNNNNRTG 5 cut(s) 16, 300, 462, 464, 756
MspA1I CMGCKG 2 cut(s) 382, 522
MspCI CTTAAG 1 cut(s) 413
MspR9I CCNGG 1 cut(s) 339
MvaI CCWGG 1 cut(s) 339
MwoI GCNNNNNNNGC 1 cut(s) 522
NcoI CCATGG 2 cut(s) 448, 757
NdeII GATC 4 cut(s) 133, 261, 372, 659
NlaIII CATG 7 cut(s) 191, 223, 256, 452, 467, 712, 761
NlaIV GGNNCC 1 cut(s) 168
NsiI ATGCAT 1 cut(s) 559
PagI TCATGA 1 cut(s) 708
PfeI GAWTC 2 cut(s) 74, 287
PflMI CCANNNNNTGG 1 cut(s) 225
PkrI GCNGC 1 cut(s) 524
PleI GAGTC 2 cut(s) 60, 622
PpsI GAGTC 2 cut(s) 60, 622
Psp124BI GAGCTC 2 cut(s) 361, 435
Psp6I CCWGG 1 cut(s) 337
PspFI CCCAGC 1 cut(s) 595
PspGI CCWGG 1 cut(s) 337
PspN4I GGNNCC 1 cut(s) 168
PspPI GGNCC 2 cut(s) 166, 593
PsuI RGATCY 3 cut(s) 261, 372, 659
PvuII CAGCTG 2 cut(s) 382, 522
RsaI GTAC 1 cut(s) 29
RsaNI GTAC 1 cut(s) 28
RseI CAYNNNNRTG 5 cut(s) 16, 300, 462, 464, 756
SacI GAGCTC 2 cut(s) 361, 435
SaqAI TTAA 2 cut(s) 414, 420
SatI GCNGC 1 cut(s) 523
Sau3AI GATC 4 cut(s) 133, 261, 372, 659
Sau96I GGNCC 2 cut(s) 166, 593
SchI GAGTC 2 cut(s) 61, 623
ScrFI CCNGG 1 cut(s) 339
SduI GDGCHC 2 cut(s) 361, 435
SexAI ACCWGGT 1 cut(s) 337
SfaNI GCATC 2 cut(s) 391, 544
SmiMI CAYNNNNRTG 5 cut(s) 16, 300, 462, 464, 756
SmlI CTYRAG 3 cut(s) 273, 413, 434
SmoI CTYRAG 3 cut(s) 273, 413, 434
Sse9I AATT 7 cut(s) 6, 65, 79, 151, 175, 405, 545
SstI GAGCTC 2 cut(s) 361, 435
StyD4I CCNGG 1 cut(s) 337
StyI CCWWGG 2 cut(s) 448, 757
TaaI ACNGT 1 cut(s) 442
TaiI ACGT 1 cut(s) 33
TaqI TCGA 1 cut(s) 674
TasI AATT 7 cut(s) 6, 65, 79, 151, 175, 405, 545
TfiI GAWTC 2 cut(s) 74, 287
Tru1I TTAA 2 cut(s) 414, 420
Tru9I TTAA 2 cut(s) 414, 420
TseI GCWGC 1 cut(s) 522
TspDTI ATGAA 4 cut(s) 176, 236, 452, 725
Van91I CCANNNNNTGG 1 cut(s) 225
Vha464I CTTAAG 1 cut(s) 413
XapI RAATTY 5 cut(s) 65, 79, 151, 175, 545
Zsp2I ATGCAT 1 cut(s) 559
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.