MD06G1195500.v1.1

Thioredoxin-like 1-2, chloroplastic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0010978
Physical Location & Seq
Reverse (-)
32972249 .. 32974122
1874 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1195500.v1.1.491

Sequence Viewer

Length: 846 bp
ATGGCCAATGCATTTGCATCACAGAGTGGTTTCTGCATTTCTTCTGGGTTGGATGAAATTGCAGTTGGCTCCAAATCCCCCAAAGGGGATTTTAGGGTTTCTCCTACCTCCCAATTTAAGGATGCAAATTCAAGAGCTTTTCCTGCATTGAGTGTTGATTTCTTGGGAAAACCCTTGATTCTCTCAGATCATAAGGGTTTCGAGAATTGGAGGGTCACAAAACCTAGCAATTTCTCTGTGCATGCACAAGCATCTCTGTGTGTAAGCAGAGCAATGAGATGGTGGGAGAAGACCCTTCACCCCAACATGGTGGAGATCCATTCTGCTCAAGAACTTGTTGATTCTTTGAAGAATGCTGGAGACAGATTGGTCGTGGTTGACTTTTACTCACCTGGTTGTGGAGGCTGCAGAGCTCTCCATCCCAAGATCTGTCAGCTGGCTGAATCGAACCCGAATGCGATTTTCCTCAAAGTTAACTATGAAGAGCACAAGACTATGTGCCATGCTCTCAACATTCATGTGCTGCCCTTCTTTAGATTCTATAGAGGTGCAGATGGTCGTGTGTGTAGCTTTAGCTGCACCAATGCAACTATTAAGAAATTCAAAGATGCATTGGCAAAACACGGGGGTGATCGTTGTAATCGTGGGCCGGCGAAAGGGTTAGATGAATCTGAGATACTAAAGTTAGCTTCAATAGGTGAAATATTAACAACAACATCATCGCCTTCCATGAAGGAAGAAAGATTGGAGGATCTGGTCACGAAAAGCGGAGATTTTTCCGGTGTTTGGGGCAATGCAAGCCACATCATAGAACTTGAGGAAGAAAGTTCCATGGTGAAAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Thioredoxin

Protein Analysis

282

Amino Acids

30.86

Weight (kDa)

7.61

Isoelectric Point (pI)

42.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thioredoxin PF00085 110 - 187 1.1e-13 Thioredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010978)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G61440 AT5G61440
fragaria_vesca FvH4_5g14700
malus_domestica MD06G1195500.v1.1 MD14G1202600.v1.1 MD16G1196100.v1.1
prunus_persica Prupe.5G195400_v2.0.a1 Prupe.5G195400_v2.0.a1
pyrus_communis pycom06g17270 pycom14g16750
rosa_chinensis RchiOBHm_Chr7g0181691
rosa_laevigata RLG00000005176
rosa_multiflora Rmu_sc0000988.1_g000011
rosa_roxburghii Rroxscaffold_3G00271800
rosa_rugosa Rorug06G0442800
rosa_samantha Rh7AG045300 Rh7BG044500 Rh7CG046000 Rh7DG044900
rosa_wichuraiana Rw7G003770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 368
AciI CCGC 1 cut(s) 768
AclWI GGATC 2 cut(s) 310, 759
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 2 cut(s) 127, 599
AdeI CACNNNGTG 1 cut(s) 26
AfiI CCNNNNNNNGG 7 cut(s) 84, 85, 118, 307, 398, 656, 786
AgsI TTSAA 4 cut(s) 132, 349, 604, 693
AjnI CCWGG 1 cut(s) 391
AjuI GAANNNNNNNTTGG 2 cut(s) 48, 80
AleI CACNNNNGTG 1 cut(s) 627
AluBI AGCT 6 cut(s) 137, 413, 436, 570, 576, 689
AluI AGCT 6 cut(s) 137, 413, 436, 570, 576, 689
Alw21I GWGCWC 2 cut(s) 415, 489
Alw26I GTCTC 1 cut(s) 354
AlwI GGATC 2 cut(s) 310, 759
AoxI GGCC 2 cut(s) 3, 647
ApeKI GCWGC 3 cut(s) 405, 523, 576
ApoI RAATTY 2 cut(s) 127, 599
AspS9I GGNCC 1 cut(s) 647
AsuHPI GGTGA 4 cut(s) 290, 381, 641, 710
BaeI ACNNNNGTAYC 2 cut(s) 668, 701
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 1 cut(s) 415
BbsI GAAGAC 1 cut(s) 296
Bbv12I GWGCWC 2 cut(s) 415, 489
BbvI GCAGC 3 cut(s) 392, 510, 563
BccI CCATC 3 cut(s) 273, 426, 548
BciT130I CCWGG 1 cut(s) 393
BcoDI GTCTC 1 cut(s) 354
BfaI CTAG 1 cut(s) 225
BfmI CTRYAG 2 cut(s) 406, 541
BglII AGATCT 1 cut(s) 426
BisI GCNGC 3 cut(s) 406, 524, 577
BlsI GCNGC 3 cut(s) 407, 525, 578
Bme1390I CCNGG 1 cut(s) 393
BmgT120I GGNCC 1 cut(s) 647
BmiI GGNNCC 1 cut(s) 70
BmrFI CCNGG 1 cut(s) 393
BmsI GCATC 4 cut(s) 26, 112, 260, 598
BpiI GAAGAC 1 cut(s) 296
BpmI CTGGAG 1 cut(s) 378
BpuEI CTTGAG 2 cut(s) 312, 836
BsaJI CCNNGG 1 cut(s) 831
BsaWI WCCGGW 1 cut(s) 779
Bsc4I CCNNNNNNNGG 7 cut(s) 84, 85, 118, 307, 398, 656, 786
Bse118I RCCGGY 1 cut(s) 649
Bse3DI GCAATG 2 cut(s) 279, 799
BseBI CCWGG 1 cut(s) 393
BseDI CCNNGG 1 cut(s) 831
BseGI GGATG 3 cut(s) 58, 127, 418
BseLI CCNNNNNNNGG 7 cut(s) 84, 85, 118, 307, 398, 656, 786
BseMI GCAATG 2 cut(s) 279, 799
BseMII CTCAG 2 cut(s) 198, 663
BseXI GCAGC 3 cut(s) 392, 510, 563
BsgI GTGCAG 2 cut(s) 562, 570
BshFI GGCC 2 cut(s) 5, 649
BsiHKAI GWGCWC 2 cut(s) 415, 489
BsiSI CCGG 2 cut(s) 650, 780
BslI CCNNNNNNNGG 7 cut(s) 84, 85, 118, 307, 398, 656, 786
BsmAI GTCTC 1 cut(s) 354
BsmI GAATGC 2 cut(s) 358, 460
BsnI GGCC 2 cut(s) 5, 649
Bsp1286I GDGCHC 2 cut(s) 415, 489
Bsp143I GATC 5 cut(s) 187, 315, 426, 631, 751
Bsp19I CCATGG 1 cut(s) 831
BspACI CCGC 1 cut(s) 768
BspANI GGCC 2 cut(s) 5, 649
BspCNI CTCAG 2 cut(s) 197, 664
BspLI GGNNCC 1 cut(s) 70
BspMAI CTGCAG 1 cut(s) 410
BspPI GGATC 2 cut(s) 310, 759
BspQI GCTCTTC 1 cut(s) 477
BsrDI GCAATG 2 cut(s) 279, 799
BsrFI RCCGGY 1 cut(s) 649
BssAI RCCGGY 1 cut(s) 649
BssECI CCNNGG 1 cut(s) 831
BssMI GATC 5 cut(s) 187, 315, 426, 631, 751
BssT1I CCWWGG 1 cut(s) 831
Bst2UI CCWGG 1 cut(s) 393
Bst6I CTCTTC 1 cut(s) 477
BstC8I GCNNGC 4 cut(s) 243, 438, 651, 799
BstDEI CTNAG 2 cut(s) 184, 672
BstDSI CCRYGG 1 cut(s) 831
BstF5I GGATG 3 cut(s) 58, 127, 418
BstKTI GATC 5 cut(s) 190, 318, 429, 634, 754
BstMAI GTCTC 1 cut(s) 354
BstMBI GATC 5 cut(s) 187, 315, 426, 631, 751
BstMWI GCNNNNNNNGC 3 cut(s) 143, 576, 798
BstNI CCWGG 1 cut(s) 393
BstNSI RCATGY 1 cut(s) 245
BstSCI CCNGG 1 cut(s) 391
BstSFI CTRYAG 2 cut(s) 406, 541
BstV1I GCAGC 3 cut(s) 392, 510, 563
BstV2I GAAGAC 1 cut(s) 296
BstX2I RGATCY 3 cut(s) 315, 426, 751
BstXI CCANNNNNNTGG 1 cut(s) 310
BstYI RGATCY 3 cut(s) 315, 426, 751
BsuRI GGCC 2 cut(s) 5, 649
BtgI CCRYGG 1 cut(s) 831
BtgZI GCGATG 1 cut(s) 705
BtsCI GGATG 3 cut(s) 58, 127, 418
Cac8I GCNNGC 4 cut(s) 243, 438, 651, 799
Cfr10I RCCGGY 1 cut(s) 649
Cfr13I GGNCC 1 cut(s) 647
CsiI ACCWGGT 1 cut(s) 391
CviAII CATG 6 cut(s) 242, 307, 503, 518, 730, 832
DdeI CTNAG 2 cut(s) 184, 672
DpnI GATC 5 cut(s) 189, 317, 428, 633, 753
DpnII GATC 5 cut(s) 187, 315, 426, 631, 751
DraIII CACNNNGTG 1 cut(s) 26
DrdI GACNNNNNNGTC 1 cut(s) 368
DseDI GACNNNNNNGTC 1 cut(s) 368
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 477
EarI CTCTTC 1 cut(s) 477
Ecl136II GAGCTC 1 cut(s) 413
Eco130I CCWWGG 1 cut(s) 831
Eco24I GRGCYC 1 cut(s) 415
Eco53kI GAGCTC 1 cut(s) 413
EcoICRI GAGCTC 1 cut(s) 413
EcoRII CCWGG 1 cut(s) 391
EcoT14I CCWWGG 1 cut(s) 831
EcoT22I ATGCAT 2 cut(s) 13, 613
EcoT38I GRGCYC 1 cut(s) 415
ErhI CCWWGG 1 cut(s) 831
FaeI CATG 6 cut(s) 245, 310, 506, 521, 733, 835
FatI CATG 6 cut(s) 241, 306, 502, 517, 729, 831
Fnu4HI GCNGC 3 cut(s) 406, 524, 577
FokI GGATG 3 cut(s) 65, 134, 405
FriOI GRGCYC 1 cut(s) 415
Fsp4HI GCNGC 3 cut(s) 406, 524, 577
FspBI CTAG 1 cut(s) 225
GluI GCNGC 3 cut(s) 406, 524, 577
GsuI CTGGAG 1 cut(s) 378
HaeIII GGCC 2 cut(s) 5, 649
HapII CCGG 2 cut(s) 650, 780
Hin1II CATG 6 cut(s) 245, 310, 506, 521, 733, 835
HincII GTYRAC 2 cut(s) 379, 475
HindII GTYRAC 2 cut(s) 379, 475
HinfI GANTC 5 cut(s) 178, 341, 443, 537, 668
HpaI GTTAAC 1 cut(s) 475
HpaII CCGG 2 cut(s) 650, 780
HphI GGTGA 4 cut(s) 290, 381, 641, 710
Hpy166II GTNNAC 2 cut(s) 379, 475
Hpy188I TCNGA 2 cut(s) 187, 673
Hpy188III TCNNGA 4 cut(s) 132, 202, 329, 760
Hpy8I GTNNAC 2 cut(s) 379, 475
HpyAV CCTTC 4 cut(s) 305, 538, 727, 735
HpyF10VI GCNNNNNNNGC 3 cut(s) 143, 576, 798
HpyF3I CTNAG 2 cut(s) 184, 672
Hsp92II CATG 6 cut(s) 245, 310, 506, 521, 733, 835
KroI GCCGGC 1 cut(s) 649
KroNI GCCGGC 1 cut(s) 651
KspAI GTTAAC 1 cut(s) 475
Kzo9I GATC 5 cut(s) 187, 315, 426, 631, 751
LguI GCTCTTC 1 cut(s) 477
LmnI GCTCC 1 cut(s) 74
LpnPI CCDG 9 cut(s) 30, 156, 342, 378, 405, 422, 663, 740, 793
Lsp1109I GCAGC 3 cut(s) 392, 510, 563
LweI GCATC 4 cut(s) 26, 112, 260, 598
MabI ACCWGGT 1 cut(s) 391
MaeI CTAG 1 cut(s) 225
MaeIII GTNAC 2 cut(s) 214, 757
MalI GATC 5 cut(s) 189, 317, 428, 633, 753
MboI GATC 5 cut(s) 187, 315, 426, 631, 751
MboII GAAGA 6 cut(s) 33, 301, 361, 494, 749, 833
MflI RGATCY 3 cut(s) 315, 426, 751
MhlI GDGCHC 2 cut(s) 415, 489
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 6 cut(s) 57, 113, 127, 205, 229, 599
MluNI TGGCCA 1 cut(s) 5
MmeI TCCRAC 1 cut(s) 30
MnlI CCTC 7 cut(s) 118, 204, 395, 476, 539, 742, 811
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 2 cut(s) 13, 613
MroNI GCCGGC 1 cut(s) 649
MscI TGGCCA 1 cut(s) 5
MseI TTAA 5 cut(s) 117, 474, 594, 707, 844
MslI CAYNNNNRTG 3 cut(s) 256, 518, 627
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 436
MspI CCGG 2 cut(s) 650, 780
MspR9I CCNGG 1 cut(s) 393
Mva1269I GAATGC 2 cut(s) 358, 460
MvaI CCWGG 1 cut(s) 393
MwoI GCNNNNNNNGC 3 cut(s) 143, 576, 798
NaeI GCCGGC 1 cut(s) 651
NcoI CCATGG 1 cut(s) 831
NdeII GATC 5 cut(s) 187, 315, 426, 631, 751
NgoMIV GCCGGC 1 cut(s) 649
NlaIII CATG 6 cut(s) 245, 310, 506, 521, 733, 835
NlaIV GGNNCC 1 cut(s) 70
NmuCI GTSAC 2 cut(s) 214, 757
NsiI ATGCAT 2 cut(s) 13, 613
NspI RCATGY 1 cut(s) 245
OliI CACNNNNGTG 1 cut(s) 627
PaeI GCATGC 1 cut(s) 245
PciSI GCTCTTC 1 cut(s) 477
PcsI WCGNNNNNNNCGW 1 cut(s) 640
PctI GAATGC 2 cut(s) 358, 460
PdiI GCCGGC 1 cut(s) 651
PfeI GAWTC 5 cut(s) 178, 341, 443, 537, 668
PkrI GCNGC 3 cut(s) 407, 525, 578
Psp124BI GAGCTC 1 cut(s) 415
Psp6I CCWGG 1 cut(s) 391
PspGI CCWGG 1 cut(s) 391
PspN4I GGNNCC 1 cut(s) 70
PspPI GGNCC 1 cut(s) 647
PstI CTGCAG 1 cut(s) 410
PsuI RGATCY 3 cut(s) 315, 426, 751
PvuII CAGCTG 1 cut(s) 436
RseI CAYNNNNRTG 3 cut(s) 256, 518, 627
SacI GAGCTC 1 cut(s) 415
SapI GCTCTTC 1 cut(s) 477
SaqAI TTAA 5 cut(s) 117, 474, 594, 707, 844
SatI GCNGC 3 cut(s) 406, 524, 577
Sau3AI GATC 5 cut(s) 187, 315, 426, 631, 751
Sau96I GGNCC 1 cut(s) 647
ScrFI CCNGG 1 cut(s) 393
SduI GDGCHC 2 cut(s) 415, 489
SexAI ACCWGGT 1 cut(s) 391
SfaNI GCATC 4 cut(s) 26, 112, 260, 598
SfcI CTRYAG 2 cut(s) 406, 541
SmiMI CAYNNNNRTG 3 cut(s) 256, 518, 627
SmlI CTYRAG 2 cut(s) 327, 815
SmoI CTYRAG 2 cut(s) 327, 815
SphI GCATGC 1 cut(s) 245
Sse9I AATT 6 cut(s) 57, 113, 127, 205, 229, 599
SsiI CCGC 1 cut(s) 768
SspI AATATT 1 cut(s) 705
SspMI CTAG 1 cut(s) 225
SstI GAGCTC 1 cut(s) 415
StyD4I CCNGG 1 cut(s) 391
StyI CCWWGG 1 cut(s) 831
TaqI TCGA 2 cut(s) 201, 446
TasI AATT 6 cut(s) 57, 113, 127, 205, 229, 599
TfiI GAWTC 5 cut(s) 178, 341, 443, 537, 668
Tru1I TTAA 5 cut(s) 117, 474, 594, 707, 844
Tru9I TTAA 5 cut(s) 117, 474, 594, 707, 844
TseFI GTSAC 2 cut(s) 214, 757
TseI GCWGC 3 cut(s) 405, 523, 576
Tsp45I GTSAC 2 cut(s) 214, 757
TspDTI ATGAA 5 cut(s) 69, 495, 506, 681, 746
XapI RAATTY 2 cut(s) 127, 599
XceI RCATGY 1 cut(s) 245
XspI CTAG 1 cut(s) 225
Zsp2I ATGCAT 2 cut(s) 13, 613
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.