Rh7BG044500

Thioredoxin-like 1-2, chloroplastic

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0010978
Physical Location & Seq
Reverse (-)
2788339 .. 2790056
1718 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG044500.1

Sequence Viewer

Length: 585 bp
ATGAAATGGTGGGAGAAAACCCTAAAACCCAACATGGTGGAGATCCATTCTGCTCAAGAACTCATTGATTCTTTGCATAATGCTGGTGATAGATTGGTTGTGGTGGATTTTTACTCACCTGGTTGTGGAGGTTGTAGAGCTCTCCATCCCAAGATCTGTCAGCTGGCTGAACAGAACCCAGATGCAATTTTTCTTAAGGTTAACTATGAGGAGCTCAAGTCTATGTGCCATGGTCTCCACATTCATGTGCTACCATTCTTTAGGTTTTATAGAGGCGCAGAAGGGCGAGTTTGTAGCTTCAGCTGCACCAATGCCACTATCAAGAAATTCAAAGATGCATTAGCTAAATATGGGGCTGAGGGTTGTAGTCTTGGCCCAGCAAAAGGCTTAGATGAGTCTGAGATATTACACTTAGCTGAGATAGGTGAAATATCAGCAAGATCTTCTCCACCATCAACCAAAGAAGAGAGAGTGGAGGAGGATTTGGTCATGAAAAGCGTAGATATGTCTGGTGTTTGGAGCAATGTAATCCATAACCATGGCATAGAACTCGGAGAAGAAGAAAGTGCCTTGCTGAAGGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Thioredoxin

Protein Analysis

194

Amino Acids

21.55

Weight (kDa)

5.71

Isoelectric Point (pI)

48.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thioredoxin PF00085 25 - 96 3.1e-13 Thioredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010978)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G61440 AT5G61440
fragaria_vesca FvH4_5g14700
malus_domestica MD06G1195500.v1.1 MD14G1202600.v1.1 MD16G1196100.v1.1
prunus_persica Prupe.5G195400_v2.0.a1 Prupe.5G195400_v2.0.a1
pyrus_communis pycom06g17270 pycom14g16750
rosa_chinensis RchiOBHm_Chr7g0181691
rosa_laevigata RLG00000005176
rosa_multiflora Rmu_sc0000988.1_g000011
rosa_roxburghii Rroxscaffold_3G00271800
rosa_rugosa Rorug06G0442800
rosa_samantha Rh7AG045300 Rh7BG044500 Rh7CG046000 Rh7DG044900
rosa_wichuraiana Rw7G003770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 37
AcsI RAATTY 1 cut(s) 326
AcuI CTGAAG 1 cut(s) 283
AfiI CCNNNNNNNGG 2 cut(s) 125, 383
AflII CTTAAG 1 cut(s) 194
AgsI TTSAA 1 cut(s) 331
AjnI CCWGG 1 cut(s) 118
AluBI AGCT 7 cut(s) 140, 163, 214, 297, 303, 344, 416
AluI AGCT 7 cut(s) 140, 163, 214, 297, 303, 344, 416
Alw21I GWGCWC 2 cut(s) 142, 216
Alw26I GTCTC 1 cut(s) 239
AlwI GGATC 1 cut(s) 37
AoxI GGCC 1 cut(s) 373
ApeKI GCWGC 1 cut(s) 303
ApoI RAATTY 1 cut(s) 326
AspLEI GCGC 1 cut(s) 278
AspS9I GGNCC 1 cut(s) 374
AsuHPI GGTGA 3 cut(s) 98, 108, 437
BanII GRGCYC 2 cut(s) 142, 216
Bbv12I GWGCWC 2 cut(s) 142, 216
BbvCI CCTCAGC 1 cut(s) 357
BbvI GCAGC 1 cut(s) 290
BccI CCATC 2 cut(s) 153, 460
BcgI CGANNNNNNTGC 2 cut(s) 532, 566
BciT130I CCWGG 1 cut(s) 120
BcoDI GTCTC 1 cut(s) 239
BfrI CTTAAG 1 cut(s) 194
BglII AGATCT 2 cut(s) 153, 440
BisI GCNGC 1 cut(s) 304
BlsI GCNGC 1 cut(s) 305
Bme1390I CCNGG 1 cut(s) 120
BmgT120I GGNCC 1 cut(s) 374
BmrFI CCNGG 1 cut(s) 120
BmsI GCATC 2 cut(s) 172, 325
Bpu10I CCTNAGC 1 cut(s) 357
BpuEI CTTGAG 2 cut(s) 39, 200
BsaI GGTCTC 1 cut(s) 239
BsaJI CCNNGG 2 cut(s) 229, 538
BsaXI ACNNNNNCTCC 2 cut(s) 203, 233
Bsc4I CCNNNNNNNGG 2 cut(s) 125, 383
Bse3DI GCAATG 1 cut(s) 529
BseBI CCWGG 1 cut(s) 120
BseDI CCNNGG 2 cut(s) 229, 538
BseGI GGATG 1 cut(s) 145
BseLI CCNNNNNNNGG 2 cut(s) 125, 383
BseMI GCAATG 1 cut(s) 529
BseMII CTCAG 3 cut(s) 348, 390, 408
BseRI GAGGAG 2 cut(s) 224, 491
BseXI GCAGC 1 cut(s) 290
BseYI CCCAGC 1 cut(s) 376
BsgI GTGCAG 1 cut(s) 289
BshFI GGCC 1 cut(s) 375
BsiHKAI GWGCWC 2 cut(s) 142, 216
BslI CCNNNNNNNGG 2 cut(s) 125, 383
BsmAI GTCTC 1 cut(s) 239
BsnI GGCC 1 cut(s) 375
Bso31I GGTCTC 1 cut(s) 239
Bsp1286I GDGCHC 2 cut(s) 142, 216
Bsp143I GATC 3 cut(s) 42, 153, 440
Bsp19I CCATGG 2 cut(s) 229, 538
BspANI GGCC 1 cut(s) 375
BspCNI CTCAG 3 cut(s) 349, 391, 409
BspHI TCATGA 1 cut(s) 489
BspPI GGATC 1 cut(s) 37
BspTI CTTAAG 1 cut(s) 194
BspTNI GGTCTC 1 cut(s) 239
BsrDI GCAATG 1 cut(s) 529
BssECI CCNNGG 2 cut(s) 229, 538
BssMI GATC 3 cut(s) 42, 153, 440
BssT1I CCWWGG 2 cut(s) 229, 538
Bst2UI CCWGG 1 cut(s) 120
Bst6I CTCTTC 1 cut(s) 459
BstAFI CTTAAG 1 cut(s) 194
BstC8I GCNNGC 1 cut(s) 165
BstDEI CTNAG 5 cut(s) 357, 388, 399, 412, 417
BstDSI CCRYGG 2 cut(s) 229, 538
BstF5I GGATG 1 cut(s) 145
BstHHI GCGC 1 cut(s) 278
BstKTI GATC 3 cut(s) 45, 156, 443
BstMAI GTCTC 1 cut(s) 239
BstMBI GATC 3 cut(s) 42, 153, 440
BstMWI GCNNNNNNNGC 1 cut(s) 303
BstNI CCWGG 1 cut(s) 120
BstSCI CCNGG 1 cut(s) 118
BstV1I GCAGC 1 cut(s) 290
BstX2I RGATCY 3 cut(s) 42, 153, 440
BstXI CCANNNNNNTGG 2 cut(s) 37, 539
BstYI RGATCY 3 cut(s) 42, 153, 440
BsuRI GGCC 1 cut(s) 375
BtgI CCRYGG 2 cut(s) 229, 538
BtsCI GGATG 1 cut(s) 145
Cac8I GCNNGC 1 cut(s) 165
CciI TCATGA 1 cut(s) 489
CfoI GCGC 1 cut(s) 278
Cfr13I GGNCC 1 cut(s) 374
CsiI ACCWGGT 1 cut(s) 118
CviAII CATG 5 cut(s) 34, 230, 245, 490, 539
DdeI CTNAG 5 cut(s) 357, 388, 399, 412, 417
DpnI GATC 3 cut(s) 44, 155, 442
DpnII GATC 3 cut(s) 42, 153, 440
Eam1104I CTCTTC 1 cut(s) 459
EarI CTCTTC 1 cut(s) 459
Ecl136II GAGCTC 2 cut(s) 140, 214
Eco130I CCWWGG 2 cut(s) 229, 538
Eco24I GRGCYC 2 cut(s) 142, 216
Eco31I GGTCTC 1 cut(s) 239
Eco53kI GAGCTC 2 cut(s) 140, 214
Eco57I CTGAAG 1 cut(s) 283
EcoICRI GAGCTC 2 cut(s) 140, 214
EcoRII CCWGG 1 cut(s) 118
EcoT14I CCWWGG 2 cut(s) 229, 538
EcoT22I ATGCAT 1 cut(s) 340
EcoT38I GRGCYC 2 cut(s) 142, 216
ErhI CCWWGG 2 cut(s) 229, 538
FaeI CATG 5 cut(s) 37, 233, 248, 493, 542
FatI CATG 5 cut(s) 33, 229, 244, 489, 538
Fnu4HI GCNGC 1 cut(s) 304
FokI GGATG 1 cut(s) 132
FriOI GRGCYC 2 cut(s) 142, 216
Fsp4HI GCNGC 1 cut(s) 304
GlaI GCGC 1 cut(s) 277
GluI GCNGC 1 cut(s) 304
GsaI CCCAGC 1 cut(s) 380
HaeIII GGCC 1 cut(s) 375
HhaI GCGC 1 cut(s) 278
Hin1II CATG 5 cut(s) 37, 233, 248, 493, 542
Hin6I GCGC 1 cut(s) 276
HinP1I GCGC 1 cut(s) 276
HincII GTYRAC 1 cut(s) 202
HindII GTYRAC 1 cut(s) 202
HinfI GANTC 2 cut(s) 68, 395
HpaI GTTAAC 1 cut(s) 202
HphI GGTGA 3 cut(s) 98, 108, 437
Hpy166II GTNNAC 1 cut(s) 202
Hpy188I TCNGA 2 cut(s) 400, 554
Hpy188III TCNNGA 3 cut(s) 56, 322, 490
Hpy8I GTNNAC 1 cut(s) 202
HpyAV CCTTC 2 cut(s) 275, 571
HpyCH4V TGCA 4 cut(s) 76, 185, 306, 338
HpyF10VI GCNNNNNNNGC 1 cut(s) 303
HpyF3I CTNAG 5 cut(s) 357, 388, 399, 412, 417
Hsp92II CATG 5 cut(s) 37, 233, 248, 493, 542
HspAI GCGC 1 cut(s) 276
KspAI GTTAAC 1 cut(s) 202
Kzo9I GATC 3 cut(s) 42, 153, 440
LmnI GCTCC 2 cut(s) 211, 519
LpnPI CCDG 7 cut(s) 69, 105, 132, 149, 192, 390, 495
Lsp1109I GCAGC 1 cut(s) 290
LweI GCATC 2 cut(s) 172, 325
MabI ACCWGGT 1 cut(s) 118
MalI GATC 3 cut(s) 44, 155, 442
MboI GATC 3 cut(s) 42, 153, 440
MboII GAAGA 4 cut(s) 435, 476, 569, 572
MflI RGATCY 3 cut(s) 42, 153, 440
MhlI GDGCHC 2 cut(s) 142, 216
MluCI AATT 2 cut(s) 186, 326
MlyI GAGTC 1 cut(s) 404
MnlI CCTC 6 cut(s) 122, 202, 266, 352, 469, 472
Mph1103I ATGCAT 1 cut(s) 340
MseI TTAA 2 cut(s) 195, 201
MslI CAYNNNNRTG 3 cut(s) 243, 245, 537
MspA1I CMGCKG 2 cut(s) 163, 303
MspCI CTTAAG 1 cut(s) 194
MspR9I CCNGG 1 cut(s) 120
MvaI CCWGG 1 cut(s) 120
MwoI GCNNNNNNNGC 1 cut(s) 303
NcoI CCATGG 2 cut(s) 229, 538
NdeII GATC 3 cut(s) 42, 153, 440
NlaIII CATG 5 cut(s) 37, 233, 248, 493, 542
NsiI ATGCAT 1 cut(s) 340
PagI TCATGA 1 cut(s) 489
PfeI GAWTC 1 cut(s) 68
PkrI GCNGC 1 cut(s) 305
PleI GAGTC 1 cut(s) 403
PpsI GAGTC 1 cut(s) 403
Psp124BI GAGCTC 2 cut(s) 142, 216
Psp6I CCWGG 1 cut(s) 118
PspFI CCCAGC 1 cut(s) 376
PspGI CCWGG 1 cut(s) 118
PspPI GGNCC 1 cut(s) 374
PsuI RGATCY 3 cut(s) 42, 153, 440
PvuII CAGCTG 2 cut(s) 163, 303
RseI CAYNNNNRTG 3 cut(s) 243, 245, 537
SacI GAGCTC 2 cut(s) 142, 216
SaqAI TTAA 2 cut(s) 195, 201
SatI GCNGC 1 cut(s) 304
Sau3AI GATC 3 cut(s) 42, 153, 440
Sau96I GGNCC 1 cut(s) 374
SchI GAGTC 1 cut(s) 404
ScrFI CCNGG 1 cut(s) 120
SduI GDGCHC 2 cut(s) 142, 216
SexAI ACCWGGT 1 cut(s) 118
SfaNI GCATC 2 cut(s) 172, 325
SmiMI CAYNNNNRTG 3 cut(s) 243, 245, 537
SmlI CTYRAG 3 cut(s) 54, 194, 215
SmoI CTYRAG 3 cut(s) 54, 194, 215
Sse9I AATT 2 cut(s) 186, 326
SstI GAGCTC 2 cut(s) 142, 216
StyD4I CCNGG 1 cut(s) 118
StyI CCWWGG 2 cut(s) 229, 538
TasI AATT 2 cut(s) 186, 326
TfiI GAWTC 1 cut(s) 68
Tru1I TTAA 2 cut(s) 195, 201
Tru9I TTAA 2 cut(s) 195, 201
TseI GCWGC 1 cut(s) 303
TspDTI ATGAA 3 cut(s) 17, 233, 506
Vha464I CTTAAG 1 cut(s) 194
XapI RAATTY 1 cut(s) 326
Zsp2I ATGCAT 1 cut(s) 340
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.