AT5G61480
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0012250
Physical Location & Seq
Reverse (-)
24724337 .. 24728244
3908 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G61480.1

Sequence Viewer

Length: 3126 bp
ATGAAAAAGAAGAACATTTCTCCTTCTCTTGTTCTTCATCCCCTTCTCCTTCTTCTACTTCCTTTCTTTGCTTTCAATTCCTTAGCTCTCAAGTTTTCACCTCAACTCTTGTCTCTCCTTTCCCTTAAAACATCTCTCTCTGGCCCTCCCTCTGCCTTTCAAGACTGGAAAGTCCCCGTTAACGGTCAAAACGACGCCGTTTGGTGTTCTTGGTCCGGTGTAGTCTGTGATAATGTAACGGCTCAAGTCATTTCCCTCGACCTCTCTCACCGGAACCTCTCTGGTCGTATTCCTATACAGATTCGTTACTTGTCGAGCTTACTCTACTTAAATCTCAGTGGGAATTCTTTGGAAGGTTCGTTTCCAACTTCTATCTTTGATCTCACCAAGCTCACTACCCTCGACATCAGCCGTAACTCGTTCGACTCGAGTTTTCCTCCCGGAATCTCCAAGCTTAAGTTCTTAAAAGTCTTCAATGCGTTCAGCAACAACTTCGAAGGTCTATTACCTAGTGACGTGTCTCGTCTTCGTTTCTTGGAAGAGCTTAACTTTGGTGGAAGTTACTTTGAAGGAGAGATTCCAGCAGCTTACGGTGGTTTACAGAGATTGAAGTTTATTCATTTAGCTGGAAATGTCCTCGGAGGTAAACTACCTCCTAGATTAGGACTCTTAACAGAGCTCCAACACATGGAAATCGGTTATAATCACTTCAACGGAAACATACCTTCGGAGTTTGCCTTACTCTCAAATCTCAAGTACTTTGACGTTTCCAATTGCAGCCTCTCTGGTTCTCTGCCTCAAGAACTCGGGAATCTCTCAAACCTAGAGACTTTATTTCTATTCCAAAACGGTTTCACCGGTGAAATCCCAGAGAGTTATAGCAACTTGAAATCCCTCAAGCTTCTCGATTTTTCGAGTAATCAGCTTTCTGGTAGTATCCCATCAGGCTTCTCGACCTTGAAGAACCTCACATGGCTAAGCTTAATCAGCAATAACCTCTCAGGTGAAGTACCTGAAGGAATCGGTGAACTCCCTGAGCTTACTACATTGTTTCTATGGAACAATAACTTCACCGGAGTTTTGCCACACAAGCTTGGATCAAACGGTAAACTTGAGACAATGGACGTCTCTAACAATTCATTCACCGGAACAATCCCTTCTTCTCTCTGCCATGGAAACAAGCTATACAAACTCATCCTCTTCTCCAACATGTTTGAAGGTGAGCTACCAAAGAGCTTGACTCGTTGCGAATCTCTATGGCGGTTTCGGAGTCAAAACAATCGATTAAACGGCACAATTCCGATCGGATTCGGCTCTCTACGTAACCTCACTTTCGTTGATTTAAGCAACAACAGATTCACCGATCAAATTCCGGCGGATTTCGCCACCGCTCCTGTTCTTCAGTACTTGAATCTCTCAACCAATTTCTTCCACAGGAAACTACCGGAAAACATATGGAAAGCTCCGAATCTACAGATCTTCTCAGCGAGTTTCAGCAATTTGATCGGTGAAATCCCAAATTACGTTGGATGCAAAAGCTTCTACAGGATTGAACTACAAGGAAACTCACTCAACGGAACGATTCCATGGGACATCGGACATTGCGAGAAGCTTCTCTGTTTGAATCTCAGCCAAAATCATCTCAACGGAATCATTCCATGGGAGATTTCAACTCTTCCGTCAATCGCCGACGTAGATCTTTCTCATAATCTCTTAACCGGAACAATCCCTTCCGATTTCGGAAGCTCTAAGACGATCACAACCTTCAACGTTTCGTATAATCAGCTAATCGGTCCGATTCCAAGTGGTTCTTTCGCTCATCTGAATCCGTCGTTCTTCTCCTCAAACGAAGGACTCTGTGGAGATCTCGTCGGAAAACCTTGCAATTCTGATAGGTTTAACGCCGGAAATGCAGATATAGACGGTCATCATAAAGAGGAACGACCTAAGAAAACAGCCGGAGCTATTGTTTGGATATTGGCGGCGGCGATTGGGGTTGGATTCTTCGTCCTTGTAGCCGCCACTAGATGCTTCCAGAAAAGCTACGGAAACAGAGTCGACGGTGGTGGAAGAAACGGCGGAGATATAGGACCGTGGAAGCTAACGGCTTTTCAGAGACTAAACTTCACGGCGGATGATGTGGTTGAGTGTCTCTCAAAGACTGATAACATCCTCGGAATGGGATCAACAGGAACAGTGTACAAAGCAGAGATGCCTAATGGAGAAATAATCGCCGTGAAAAAACTTTGGGGAAAAAACAAAGAGAACGGCAAAATCCGGCGGCGGAAGAGCGGCGTATTGGCGGAGGTTGATGTTCTAGGGAACGTACGTCACCGGAACATCGTTCGTCTCCTTGGATGTTGCACGAATCGAGATTGCACGATGCTTTTATACGAATACATGCCTAATGGAAGCTTAGACGATCTTCTTCACGGTGGGGATAAGACGATGACCGCGGCGGCGGAATGGACGGCTTTGTATCAGATCGCGATTGGAGTGGCTCAAGGGATCTGTTATCTCCACCATGATTGTGATCCGGTGATTGTACACCGTGACCTGAAACCTAGCAATATCCTCCTCGACGCCGATTTCGAGGCGCGTGTGGCGGACTTCGGCGTCGCGAAGCTTATTCAAACCGACGAATCCATGTCCGTCGTCGCCGGTTCGTACGGTTACATTGCACCAGAATATGCTTACACTTTACAAGTGGATAAAAAGAGTGATATCTATAGCTATGGAGTGATTTTATTAGAGATAATCACCGGAAAAAGATCGGTGGAACCGGAATTTGGAGAAGGTAACAGTATCGTGGATTGGGTTAGATCAAAGTTGAAGACGAAAGAAGATGTAGAAGAAGTTCTAGACAAAAGCATGGGTAGGTCGTGTAGTCTTATAAGAGAAGAGATGAAACAAATGTTGAGAATTGCGTTGTTGTGTACAAGCCGGAGTCCGACAGACAGACCGCCGATGAGAGATGTGTTGTTGATTCTTCAAGAGGCAAAGCCAAAGAGGAAGACAGTAGGGGATAATGTGATCGTCGTTGGTGATGTTAATGATGTCAATTTCGAAGATGTTTGTAGTGTTGATGTTGGTCATGATGTTAAATGTCAAAGGATTGGGGTGTGA

Protein Analysis

1041

Amino Acids

114.68

Weight (kDa)

6.4

Isoelectric Point (pI)

31.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 34 - 77 5e-06 Leucine rich repeat N-terminal domain
LRR_8 PF13855 85 - 140 7.5e-07 Leucine rich repeat
LRR_14 PF23598 248 - 351 2.5e-10 Leucine-rich repeat region
LRR_8 PF13855 345 - 404 6.3e-06 Leucine rich repeat
PK_Tyr_Ser-Thr PF07714 721 - 994 3.4e-40 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 722 - 992 2.2e-46 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012250)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G61480
fragaria_vesca FvH4_7g27270
malus_domestica MD01G1181700.v1.1 MD07G1251900.v1.1
prunus_persica Prupe.2G275500_v2.0.a1
pyrus_communis pycom01g19150 pycom07g22430
rosa_chinensis RchiOBHm_Chr1g0374101
rosa_laevigata RLG00000026763
rosa_multiflora Rmu_co8312173.1_g000001 Rmu_co8440105.1_g000001 Rmu_sc0006924.1_g000013
rosa_roxburghii Rroxscaffold_4G00283740
rosa_rugosa Rorug01G0380800
rosa_samantha Rh1AG391500 Rh1BG355500 Rh1CG368500 Rh1DG386200
rosa_wichuraiana Rw1G034410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 702, 2894
AasI GACNNNNNNGTC 2 cut(s) 170, 2615
AatII GACGTC 1 cut(s) 1128
AccB7I CCANNNNNTGG 1 cut(s) 688
AccBSI CCGCTC 2 cut(s) 1391, 2292
AccI GTMKAC 1 cut(s) 2058
AccII CGCG 4 cut(s) 2456, 2489, 2599, 2621
AclI AACGTT 1 cut(s) 1770
AclWI GGATC 4 cut(s) 1105, 2191, 2516, 2528
AcsI RAATTY 3 cut(s) 343, 1368, 2786
AcuI CTGAAG 2 cut(s) 1035, 1385
AcyI GRCGYC 4 cut(s) 195, 1125, 2583, 2616
AfaI GTAC 8 cut(s) 758, 1011, 1406, 2201, 2328, 2547, 2669, 2938
AfiI CCNNNNNNNGG 5 cut(s) 182, 662, 688, 2179, 2789
AflII CTTAAG 1 cut(s) 455
AflIII ACRYGT 2 cut(s) 516, 1209
AgeI ACCGGT 1 cut(s) 857
AjiI CACGTC 1 cut(s) 517
AjuI GAANNNNNNNTTGG 4 cut(s) 443, 475, 2772, 2804
Alw21I GWGCWC 1 cut(s) 681
Alw26I GTCTC 8 cut(s) 117, 525, 821, 1109, 1132, 2110, 2156, 2354
AlwI GGATC 4 cut(s) 1105, 2191, 2516, 2528
Ama87I CYCGRG 2 cut(s) 427, 806
AoxI GGCC 1 cut(s) 142
ApeKI GCWGC 2 cut(s) 584, 777
ApoI RAATTY 3 cut(s) 343, 1368, 2786
ArsI GACNNNNNNTTYG 2 cut(s) 2573, 2605
AsiGI ACCGGT 1 cut(s) 857
Asp700I GAANNNNTTC 1 cut(s) 2856
AspLEI GCGC 1 cut(s) 2599
AspS9I GGNCC 4 cut(s) 143, 213, 1793, 2090
AsuC2I CCSGG 1 cut(s) 441
AsuII TTCGAA 2 cut(s) 495, 3066
AvaI CYCGRG 2 cut(s) 427, 806
AvaII GGWCC 3 cut(s) 213, 1793, 2090
BanII GRGCYC 1 cut(s) 681
BarI GAAGNNNNNNTAC 4 cut(s) 489, 521, 1209, 1241
BbsI GAAGAC 4 cut(s) 463, 518, 2840, 3020
Bbv12I GWGCWC 1 cut(s) 681
BbvI GCAGC 2 cut(s) 596, 789
BccI CCATC 1 cut(s) 949
BcgI CGANNNNNNTGC 4 cut(s) 475, 509, 1486, 1520
BciVI GTATCC 1 cut(s) 947
BcnI CCSGG 1 cut(s) 441
BcoDI GTCTC 8 cut(s) 117, 525, 821, 1109, 1132, 2110, 2156, 2354
BfaI CTAG 7 cut(s) 510, 657, 824, 2025, 2318, 2565, 2861
BfmI CTRYAG 3 cut(s) 1472, 1543, 2728
BfrI CTTAAG 1 cut(s) 455
BfuI GTATCC 1 cut(s) 947
BglII AGATCT 3 cut(s) 1476, 1696, 1864
BisI GCNGC 9 cut(s) 585, 778, 1983, 1986, 2019, 2282, 2293, 2457, 2460
BlpI GCTNAGC 1 cut(s) 977
BlsI GCNGC 9 cut(s) 586, 779, 1984, 1987, 2020, 2283, 2294, 2458, 2461
BmcAI AGTACT 2 cut(s) 758, 1406
Bme1390I CCNGG 1 cut(s) 441
Bme18I GGWCC 3 cut(s) 213, 1793, 2090
BmeT110I CYCGRG 2 cut(s) 427, 806
BmgBI CACGTC 1 cut(s) 517
BmgT120I GGNCC 4 cut(s) 143, 213, 1793, 2090
BmiI GGNNCC 2 cut(s) 275, 2781
BmrFI CCNGG 1 cut(s) 441
BmsI GCATC 4 cut(s) 1520, 2018, 2202, 2373
BpiI GAAGAC 4 cut(s) 463, 518, 2840, 3020
BplI GAGNNNNNCTC 6 cut(s) 421, 453, 1225, 1257, 2138, 2170
Bpu10I CCTNAGC 2 cut(s) 82, 1035
Bpu1102I GCTNAGC 1 cut(s) 977
Bpu14I TTCGAA 2 cut(s) 495, 3066
BpuEI CTTGAG 7 cut(s) 74, 228, 737, 783, 881, 1133, 2487
BpuMI CCSGG 1 cut(s) 441
Bsa29I ATCGAT 1 cut(s) 1282
BsaAI YACGTR 1 cut(s) 1322
BsaBI GATNNNNATC 1 cut(s) 2532
BsaHI GRCGYC 4 cut(s) 195, 1125, 2583, 2616
BsaJI CCNNGG 8 cut(s) 637, 1171, 1586, 1658, 2093, 2173, 2353, 2454
BsaXI ACNNNNNCTCC 4 cut(s) 1854, 1884, 1953, 1983
Bsc4I CCNNNNNNNGG 5 cut(s) 182, 662, 688, 2179, 2789
Bse118I RCCGGY 2 cut(s) 857, 2660
Bse1I ACTGG 1 cut(s) 170
Bse3DI GCAATG 2 cut(s) 1600, 2676
Bse8I GATNNNNATC 1 cut(s) 2532
BseCI ATCGAT 1 cut(s) 1282
BseDI CCNNGG 8 cut(s) 637, 1171, 1586, 1658, 2093, 2173, 2353, 2454
BseGI GGATG 6 cut(s) 37, 1194, 1535, 2140, 2169, 2363
BseJI GATNNNNATC 1 cut(s) 2532
BseLI CCNNNNNNNGG 5 cut(s) 182, 662, 688, 2179, 2789
BseMI GCAATG 2 cut(s) 1600, 2676
BseMII CTCAG 5 cut(s) 349, 1014, 1026, 1497, 1642
BseNI ACTGG 1 cut(s) 170
BseRI GAGGAG 2 cut(s) 1831, 2567
BseXI GCAGC 2 cut(s) 596, 789
Bsh1236I CGCG 4 cut(s) 2456, 2489, 2599, 2621
Bsh1285I CGRYCG 1 cut(s) 1305
BshFI GGCC 1 cut(s) 144
BshTI ACCGGT 1 cut(s) 857
BshVI ATCGAT 1 cut(s) 1282
BsiEI CGRYCG 1 cut(s) 1305
BsiHKAI GWGCWC 1 cut(s) 681
BsiHKCI CYCGRG 2 cut(s) 427, 806
BsiWI CGTACG 2 cut(s) 2326, 2667
BslFI GGGAC 2 cut(s) 158, 1604
BslI CCNNNNNNNGG 5 cut(s) 182, 662, 688, 2179, 2789
BsmAI GTCTC 8 cut(s) 117, 525, 821, 1109, 1132, 2110, 2156, 2354
BsmBI CGTCTC 2 cut(s) 1132, 2354
BsmFI GGGAC 2 cut(s) 158, 1604
BsnI GGCC 1 cut(s) 144
BsoBI CYCGRG 2 cut(s) 427, 806
Bsp119I TTCGAA 2 cut(s) 495, 3066
Bsp1286I GDGCHC 1 cut(s) 681
Bsp1407I TGTACA 3 cut(s) 2199, 2545, 2936
Bsp1720I GCTNAGC 1 cut(s) 977
Bsp19I CCATGG 3 cut(s) 1171, 1586, 1658
Bsp68I TCGCGA 2 cut(s) 2489, 2621
BspANI GGCC 1 cut(s) 144
BspCNI CTCAG 5 cut(s) 348, 1013, 1027, 1496, 1641
BspDI ATCGAT 1 cut(s) 1282
BspFNI CGCG 4 cut(s) 2456, 2489, 2599, 2621
BspHI TCATGA 1 cut(s) 3094
BspLI GGNNCC 2 cut(s) 275, 2781
BspPI GGATC 4 cut(s) 1105, 2191, 2516, 2528
BspQI GCTCTTC 2 cut(s) 534, 2282
BspT104I TTCGAA 2 cut(s) 495, 3066
BspTI CTTAAG 1 cut(s) 455
BsrBI CCGCTC 2 cut(s) 1391, 2292
BsrDI GCAATG 2 cut(s) 1600, 2676
BsrFI RCCGGY 2 cut(s) 857, 2660
BsrGI TGTACA 3 cut(s) 2199, 2545, 2936
BsrI ACTGG 1 cut(s) 170
BssAI RCCGGY 2 cut(s) 857, 2660
BssECI CCNNGG 8 cut(s) 637, 1171, 1586, 1658, 2093, 2173, 2353, 2454
BssNI GRCGYC 4 cut(s) 195, 1125, 2583, 2616
BssT1I CCWWGG 4 cut(s) 1171, 1586, 1658, 2353
Bst6I CTCTTC 5 cut(s) 534, 1205, 1680, 2282, 2895
BstACI GRCGYC 4 cut(s) 195, 1125, 2583, 2616
BstAFI CTTAAG 1 cut(s) 455
BstAUI TGTACA 3 cut(s) 2199, 2545, 2936
BstBAI YACGTR 1 cut(s) 1322
BstBI TTCGAA 2 cut(s) 495, 3066
BstDSI CCRYGG 5 cut(s) 1171, 1586, 1658, 2093, 2454
BstENI CCTNNNNNAGG 1 cut(s) 660
BstF5I GGATG 6 cut(s) 37, 1194, 1535, 2140, 2169, 2363
BstFNI CGCG 4 cut(s) 2456, 2489, 2599, 2621
BstHHI GCGC 1 cut(s) 2599
BstMAI GTCTC 8 cut(s) 117, 525, 821, 1109, 1132, 2110, 2156, 2354
BstMCI CGRYCG 1 cut(s) 1305
BstMWI GCNNNNNNNGC 5 cut(s) 987, 1090, 1382, 1910, 2603
BstNSI RCATGY 2 cut(s) 1213, 2404
BstSCI CCNGG 1 cut(s) 439
BstSFI CTRYAG 3 cut(s) 1472, 1543, 2728
BstSNI TACGTA 1 cut(s) 1322
BstUI CGCG 4 cut(s) 2456, 2489, 2599, 2621
BstV1I GCAGC 2 cut(s) 596, 789
BstV2I GAAGAC 4 cut(s) 463, 518, 2840, 3020
BstX2I RGATCY 4 cut(s) 1476, 1696, 1864, 2508
BstYI RGATCY 4 cut(s) 1476, 1696, 1864, 2508
Bsu15I ATCGAT 1 cut(s) 1282
BsuI GTATCC 1 cut(s) 947
BsuRI GGCC 1 cut(s) 144
BsuTUI ATCGAT 1 cut(s) 1282
BtgI CCRYGG 5 cut(s) 1171, 1586, 1658, 2093, 2454
BtrI CACGTC 1 cut(s) 517
BtsCI GGATG 6 cut(s) 37, 1194, 1535, 2140, 2169, 2363
BtsIMutI CAGTG 2 cut(s) 343, 2202
BtuMI TCGCGA 2 cut(s) 2489, 2621
CciI TCATGA 1 cut(s) 3094
CfoI GCGC 1 cut(s) 2599
Cfr10I RCCGGY 2 cut(s) 857, 2660
Cfr13I GGNCC 4 cut(s) 143, 213, 1793, 2090
Cfr42I CCGCGG 1 cut(s) 2457
ClaI ATCGAT 1 cut(s) 1282
CpoI CGGWCCG 1 cut(s) 1793
CseI GACGC 3 cut(s) 203, 2591, 2605
Csp6I GTAC 8 cut(s) 757, 1010, 1405, 2200, 2327, 2546, 2668, 2937
CspAI ACCGGT 1 cut(s) 857
CspCI CAANNNNNGTGG 2 cut(s) 2510, 2545
CspI CGGWCCG 1 cut(s) 1793
CviQI GTAC 8 cut(s) 757, 1010, 1405, 2200, 2327, 2546, 2668, 2937
DrdI GACNNNNNNGTC 2 cut(s) 170, 2615
DseDI GACNNNNNNGTC 2 cut(s) 170, 2615
Eam1104I CTCTTC 5 cut(s) 534, 1205, 1680, 2282, 2895
EarI CTCTTC 5 cut(s) 534, 1205, 1680, 2282, 2895
EciI GGCGGA 7 cut(s) 1391, 2094, 2147, 2299, 2318, 2477, 2621
Ecl136II GAGCTC 1 cut(s) 679
Eco105I TACGTA 1 cut(s) 1322
Eco130I CCWWGG 4 cut(s) 1171, 1586, 1658, 2353
Eco24I GRGCYC 1 cut(s) 681
Eco32I GATATC 1 cut(s) 2725
Eco47I GGWCC 3 cut(s) 213, 1793, 2090
Eco53kI GAGCTC 1 cut(s) 679
Eco57I CTGAAG 2 cut(s) 1035, 1385
Eco88I CYCGRG 2 cut(s) 427, 806
EcoICRI GAGCTC 1 cut(s) 679
EcoNI CCTNNNNNAGG 1 cut(s) 660
EcoRI GAATTC 1 cut(s) 343
EcoRV GATATC 1 cut(s) 2725
EcoT14I CCWWGG 4 cut(s) 1171, 1586, 1658, 2353
EcoT38I GRGCYC 1 cut(s) 681
ErhI CCWWGG 4 cut(s) 1171, 1586, 1658, 2353
Esp3I CGTCTC 2 cut(s) 1132, 2354
FalI AAGNNNNNCTT 2 cut(s) 1795, 1827
FaqI GGGAC 2 cut(s) 158, 1604
FauNDI CATATG 1 cut(s) 1454
FblI GTMKAC 1 cut(s) 2058
Fnu4HI GCNGC 9 cut(s) 585, 778, 1983, 1986, 2019, 2282, 2293, 2457, 2460
FokI GGATG 6 cut(s) 24, 1181, 1542, 2147, 2156, 2370
FriOI GRGCYC 1 cut(s) 681
Fsp4HI GCNGC 9 cut(s) 585, 778, 1983, 1986, 2019, 2282, 2293, 2457, 2460
FspBI CTAG 7 cut(s) 510, 657, 824, 2025, 2318, 2565, 2861
GlaI GCGC 1 cut(s) 2598
GluI GCNGC 9 cut(s) 585, 778, 1983, 1986, 2019, 2282, 2293, 2457, 2460
HaeIII GGCC 1 cut(s) 144
HgaI GACGC 3 cut(s) 203, 2591, 2605
HhaI GCGC 1 cut(s) 2599
Hin1I GRCGYC 4 cut(s) 195, 1125, 2583, 2616
Hin6I GCGC 1 cut(s) 2597
HinP1I GCGC 1 cut(s) 2597
HincII GTYRAC 2 cut(s) 181, 2059
HindII GTYRAC 2 cut(s) 181, 2059
HindIII AAGCTT 8 cut(s) 452, 899, 979, 1091, 1537, 1610, 2413, 2624
HpaI GTTAAC 1 cut(s) 181
Hpy166II GTNNAC 9 cut(s) 181, 599, 647, 1028, 1109, 2059, 2200, 2548, 2937
Hpy8I GTNNAC 9 cut(s) 181, 599, 647, 1028, 1109, 2059, 2200, 2548, 2937
HpyCH4IV ACGT 9 cut(s) 516, 765, 1125, 1321, 1524, 1692, 1770, 2325, 2329
HpyCH4V TGCA 7 cut(s) 777, 1533, 1884, 1913, 2364, 2379, 2681
HpyF10VI GCNNNNNNNGC 5 cut(s) 987, 1090, 1382, 1910, 2603
HpySE526I ACGT 9 cut(s) 516, 765, 1125, 1321, 1524, 1692, 1770, 2325, 2329
Hsp92I GRCGYC 4 cut(s) 195, 1125, 2583, 2616
HspAI GCGC 1 cut(s) 2597
KspAI GTTAAC 1 cut(s) 181
KspI CCGCGG 1 cut(s) 2457
LguI GCTCTTC 2 cut(s) 534, 2282
LmnI GCTCC 4 cut(s) 684, 1396, 1468, 1961
Lsp1109I GCAGC 2 cut(s) 596, 789
LweI GCATC 4 cut(s) 1520, 2018, 2202, 2373
MaeI CTAG 7 cut(s) 510, 657, 824, 2025, 2318, 2565, 2861
MaeII ACGT 9 cut(s) 516, 765, 1125, 1321, 1524, 1692, 1770, 2325, 2329
MbiI CCGCTC 2 cut(s) 1391, 2292
MfeI CAATTG 1 cut(s) 772
MflI RGATCY 4 cut(s) 1476, 1696, 1864, 2508
MhlI GDGCHC 1 cut(s) 681
MlyI GAGTC 7 cut(s) 419, 660, 1234, 1279, 1848, 2064, 2956
MmeI TCCRAC 7 cut(s) 389, 706, 1230, 1507, 1852, 1978, 2975
MroXI GAANNNNTTC 1 cut(s) 2856
MslI CAYNNNNRTG 1 cut(s) 2529
MspA1I CMGCKG 1 cut(s) 2456
MspCI CTTAAG 1 cut(s) 455
MspR9I CCNGG 1 cut(s) 441
MunI CAATTG 1 cut(s) 772
MvnI CGCG 4 cut(s) 2456, 2489, 2599, 2621
MwoI GCNNNNNNNGC 5 cut(s) 987, 1090, 1382, 1910, 2603
NciI CCSGG 1 cut(s) 441
NcoI CCATGG 3 cut(s) 1171, 1586, 1658
NdeI CATATG 1 cut(s) 1454
NlaIV GGNNCC 2 cut(s) 275, 2781
NmuCI GTSAC 3 cut(s) 512, 2330, 2552
NruI TCGCGA 2 cut(s) 2489, 2621
NspI RCATGY 2 cut(s) 1213, 2404
NspV TTCGAA 2 cut(s) 495, 3066
PaeR7I CTCGAG 1 cut(s) 427
PagI TCATGA 1 cut(s) 3094
PciI ACATGT 1 cut(s) 1209
PciSI GCTCTTC 2 cut(s) 534, 2282
PcsI WCGNNNNNNNCGW 4 cut(s) 189, 425, 1602, 2589
PdmI GAANNNNTTC 1 cut(s) 2856
Pfl23II CGTACG 2 cut(s) 2326, 2667
PflMI CCANNNNNTGG 1 cut(s) 688
PfoI TCCNGGA 1 cut(s) 439
PinAI ACCGGT 1 cut(s) 857
PkrI GCNGC 9 cut(s) 586, 779, 1984, 1987, 2020, 2283, 2294, 2458, 2461
Ple19I CGATCG 1 cut(s) 1305
PleI GAGTC 7 cut(s) 419, 660, 1234, 1278, 1848, 2063, 2955
PpsI GAGTC 7 cut(s) 419, 660, 1234, 1278, 1848, 2063, 2955
Ppu21I YACGTR 1 cut(s) 1322
PscI ACATGT 1 cut(s) 1209
PsiI TTATAA 2 cut(s) 702, 2894
Psp124BI GAGCTC 1 cut(s) 681
Psp1406I AACGTT 1 cut(s) 1770
PspLI CGTACG 2 cut(s) 2326, 2667
PspN4I GGNNCC 2 cut(s) 275, 2781
PspPI GGNCC 4 cut(s) 143, 213, 1793, 2090
PspXI VCTCGAGB 1 cut(s) 427
PsrI GAACNNNNNNTAC 2 cut(s) 2841, 2873
PsuI RGATCY 4 cut(s) 1476, 1696, 1864, 2508
PvuI CGATCG 1 cut(s) 1305
RruI TCGCGA 2 cut(s) 2489, 2621
RsaI GTAC 8 cut(s) 758, 1011, 1406, 2201, 2328, 2547, 2669, 2938
RsaNI GTAC 8 cut(s) 757, 1010, 1405, 2200, 2327, 2546, 2668, 2937
RseI CAYNNNNRTG 1 cut(s) 2529
Rsr2I CGGWCCG 1 cut(s) 1793
RsrII CGGWCCG 1 cut(s) 1793
SacI GAGCTC 1 cut(s) 681
SacII CCGCGG 1 cut(s) 2457
SalI GTCGAC 1 cut(s) 2057
SapI GCTCTTC 2 cut(s) 534, 2282
SatI GCNGC 9 cut(s) 585, 778, 1983, 1986, 2019, 2282, 2293, 2457, 2460
Sau96I GGNCC 4 cut(s) 143, 213, 1793, 2090
ScaI AGTACT 2 cut(s) 758, 1406
SchI GAGTC 7 cut(s) 419, 660, 1234, 1279, 1848, 2064, 2956
ScrFI CCNGG 1 cut(s) 441
SduI GDGCHC 1 cut(s) 681
SfaNI GCATC 4 cut(s) 1520, 2018, 2202, 2373
SfcI CTRYAG 3 cut(s) 1472, 1543, 2728
Sfr274I CTCGAG 1 cut(s) 427
Sfr303I CCGCGG 1 cut(s) 2457
SfuI TTCGAA 2 cut(s) 495, 3066
SgrAI CRCCGGYG 1 cut(s) 857
SgrBI CCGCGG 1 cut(s) 2457
SinI GGWCC 3 cut(s) 213, 1793, 2090
SlaI CTCGAG 1 cut(s) 427
SmiMI CAYNNNNRTG 1 cut(s) 2529
SmlI CTYRAG 9 cut(s) 89, 243, 427, 455, 752, 798, 896, 1112, 2502
SmoI CTYRAG 9 cut(s) 89, 243, 427, 455, 752, 798, 896, 1112, 2502
SnaBI TACGTA 1 cut(s) 1322
SspMI CTAG 7 cut(s) 510, 657, 824, 2025, 2318, 2565, 2861
SstI GAGCTC 1 cut(s) 681
StyD4I CCNGG 1 cut(s) 439
StyI CCWWGG 4 cut(s) 1171, 1586, 1658, 2353
TaiI ACGT 9 cut(s) 519, 768, 1128, 1324, 1527, 1695, 1773, 2328, 2332
TaqII GACCGA 1 cut(s) 1781
TatI WGTACW 5 cut(s) 756, 1404, 2199, 2545, 2936
TauI GCSGC 7 cut(s) 1985, 1988, 2021, 2284, 2295, 2459, 2462
TscAI CASTG 2 cut(s) 343, 2202
TseFI GTSAC 3 cut(s) 512, 2330, 2552
TseI GCWGC 2 cut(s) 584, 777
Tsp45I GTSAC 3 cut(s) 512, 2330, 2552
TspDTI ATGAA 5 cut(s) 17, 26, 608, 1128, 2921
TspGWI ACGGA 7 cut(s) 729, 1590, 1662, 1668, 1818, 2061, 2641
TspRI CASTG 2 cut(s) 343, 2202
Van91I CCANNNNNTGG 1 cut(s) 688
Vha464I CTTAAG 1 cut(s) 455
VpaK11BI GGWCC 3 cut(s) 213, 1793, 2090
XagI CCTNNNNNAGG 1 cut(s) 660
XapI RAATTY 3 cut(s) 343, 1368, 2786
XbaI TCTAGA 1 cut(s) 2860
XceI RCATGY 2 cut(s) 1213, 2404
XhoI CTCGAG 1 cut(s) 427
XmiI GTMKAC 1 cut(s) 2058
XmnI GAANNNNTTC 1 cut(s) 2856
XspI CTAG 7 cut(s) 510, 657, 824, 2025, 2318, 2565, 2861
ZraI GACGTC 1 cut(s) 1126
ZrmI AGTACT 2 cut(s) 758, 1406
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.