RchiOBHm_Chr1g0374101

Leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0012250
Physical Location & Seq
Forward (+)
62028103 .. 62032499
4397 bp
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UTR
Exon/CDS
Intron
PRQ59793

Sequence Viewer

Length: 3069 bp
ATGAAACCTCCCTTTCTTCGCTTTCTCTTCTTCTTCTCTCTCTTCTTTCTTCAAGCATCCATAGTCCTCTGTGCTTCAAATTCTGCACCTTCCCAACTCAGTGCCTTGCTCTCCCTCAAATCCTCACTCAAGGATCCTCTCTTTACATTCAATGGCTGGGAGAGCAAGGCTAATACTGAAGGTCCAGTTTGGTGTTCATGGTCTGGAATCAAATGCCACCCCAACACATCCCTAGTAGTCTCTCTAGACCTCTCTCACCGCAATCTTTCCGGTACAATACCAGCTGAAATTCGCTACTTGTCGAGCTTGATTCACCTAAACTTGAGCGGAAACAGCTTTGCTGGACTTCTGCAACCAGCCATTTTCCAACTCACCCAGCTCAGAATCCTTGACATAAGCCACAACGACTTCAACTTAACTTTCCCGCCTGGGATTTCCAAGCTCAAGTTCTTGAGGGTCTTCAATGCTTACAGCAACAGCTTCACTGGTCCTTTACCTCAAGAAATCATCCGACTCAGGTTCCTCGAGAAGCTCAACCTTAGTGGAAGCTACTTCGAAGGTAATATTCCACCAGGGTATGGAAATATTCCCCAACTCCGGGAGCTTGGCCTCGCTGGAAATGTTCTTAAAGGCGCAATACCACCTCAACTGGGGTTGTTATCAGAGCTCACTCGCATGGAGATCGGCTACAACGGCTTCACAGGTGGGCTTCCGGTACAATTGACATTACTATCAAACCTCACCTACTTAGACATCTCCAATAACTTGCTCTCCGGGTCACTCCCACCTGAGCTTGGCAATTTAACCATGCTCAACACATTGTTCCTCTTCAAAAACCAATTTTCGGGCACTATCCCACCCACCCTCGGAAACTTACAAGCTCTCCAATTCCTCGACTTATCAGATAATCAGCTTTCGGGTTCCATCCCAGCCGCCATTGCTACGCTGAAACAGCTCAACAGGCTGAGCTTAATGAACAATTTTTTAGTTGGCGAAATCCCATCCCAAATCGGAGAGCTTCCCTACTTGGATACCCTCCTTCTCTGGAACAACTCCCTCACAGGGTCCCTCCCCCAAAACCTCGGCTCCAATGCGAAGCTCGTCCTCGTCGACCTCTCTTCCAACTCCCTCACAGGTCCAATTCCCCCGACTCTGTGCAAAGGCAACACACTCGTCAAGCTGATCCTCTTCTCAAACAAATTCACCAGCCATCTCCCACCAAATTTAGCCAGTTGTACATCCTTGTTCCGATTCCGCATCCAAAACAACCAACTTAACGGCTCAATCCCTCAGGGCTTCGGCTCCCTCCCCAACCTAACCTTCTTCGACCTCAGCAACAACAGCTTCTCCGGCCCCATCCCCTCCGATCTCGGCAATGCCGCGAAGCTTCAGTACCTAAACATCTCCCAGAACCCTCTCCACACACTCCTCCCATCAAACATCTGGGGCGCTGTTAATCTGCAGATATTCTCCGCCATTTCCAGCCACCTCACCGGAAAAATCCCCGACTTCATCGGCTGCAAGAGCGTCTACAAGATCGAATTACAACGCAACAACTTCACCGGCAGCATTCCCTGGGATATTGGCCACTGCGAGAAGCTTCTGAGCTTAAGCCTGAGCCGGAACTCACTCACCGGAATTATACCATGGGAAATTTCAGCTCTGCCTTCCATAACCGACCTGGACCTCTCCTACAACTTCCTCTCCGGGACCATCCCCTCGAATTTCGAGAATTGCAGAACGCTGGAGACCTTTAACGTCTCGTTTAATCTGTTAACCGGTCCTATTCCGTCTTCCGGTGCGATCTTCCCGAACCTTCATCCGACGTCGTTTTCCGGGAATGAGGGGCTATGCGGCGCCGTTTTGGCCAAGCCGTGCGCGGCGGATACTCTAACGGCTGGAGCTGTTGAGGTTGATGGTCACGAGCAGCCGAAGAAGACGGCCGGCGCGATTGTGTGGATCATGGCGGCGGCGTTCGGGATCGGTCTCTTCGTCCTCGTCGCCGGGACGAGGTGCTTCCACGCGAGCTACAGCCGTCGGATGGAGGATAGCCCCCAGATCGGACCGTGGAAATTGACCGCCTTCCAGCGTTTGAATTTCACGGCGGAGGATGTGCTGGAGTGTTTGCAGATGAGCGATAAGGTGATAGGGATGGGTGGGACCGGGACGGTGTATAAGGCGGAGATGCCAGGTGGGGAGATCATAGCGGTGAAGAAGCTGTGGGGGAAGCAGAAGGAGAGCATAATCCGACGGCGGAGAGGGGTGCTGGCCGAGGTGGAGGTGTTGGGGAACGTGAGGCATAGGAATATAGTGAGATTGTTAGGGTGCTGCTGCAACAGGGACTGCACCATGCTGCTCTACGAGTACATGCCCAATGGCAACTTGGACGACTTGTTGCATGGCAAGAACAAGGCCCAGAATTTGGGCGCGGATTGGGTCACGAGGTACAAGATTGCCCTCGGTGTGGCTCAAGGGATTTGCTACTTACATCACGACTGTGATCCGGTGATCGTCCACCGCGATCTCAAGCCGAGTAACATCCTCTTGGACGGTGACATGGAGGCGAGAGTGGCGGACTTTGGGGTGGCTAAGCTGATCCAGAGCGATGAGTCCATGTCGGTGATCGCCGGTTCCTACGGCTACATTGCACCAGAGTATGCTTATACCCTCCAAGTTGATGAGAAGAGTGACATATACAGCTTCGGGGTTGTGCTATTGGAGATTTTAAGCGGAAAGAGATCGGTGGAGGCGATTTTCGGGGATGGGAATAGCATTGTGGATTGGGTTCGGACGAAAATCAAGAGTAAAGACGGGATCAACGATGTTTTGGACAAGAATGCCGGGGCAGGTTGTACGCCGGTGAGGGAGGAGATGATGCAAATGCTTCGGATTGCGTTGCTTTGCACGAGTAGGAATCCGGCGGACCGGCCGTCCATGAGGGATGTGGTGTTGATGCTCATTGAAGCCAAACCTAAGAGGAAGTTGCTTGCTAGTCATGTTGTTGGTCGTGATCGAAGTGTGAGTTGTGGTGATATTCCTTTGCCACAAAAGCCAACAATGGAATGTTAA

Protein Analysis

1022

Amino Acids

111.62

Weight (kDa)

8.09

Isoelectric Point (pI)

34.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 30 - 72 5e-06 Leucine rich repeat N-terminal domain
LRR_8 PF13855 80 - 137 5.2e-06 Leucine rich repeat
LRR_14 PF23598 284 - 352 7.9e-07 Leucine-rich repeat region
LRR_8 PF13855 509 - 566 1.9e-07 Leucine rich repeat
Pkinase PF00069 713 - 982 3.9e-45 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 713 - 984 1.8e-39 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012250)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G61480
fragaria_vesca FvH4_7g27270
malus_domestica MD01G1181700.v1.1 MD07G1251900.v1.1
prunus_persica Prupe.2G275500_v2.0.a1
pyrus_communis pycom01g19150 pycom07g22430
rosa_chinensis RchiOBHm_Chr1g0374101
rosa_laevigata RLG00000026763
rosa_multiflora Rmu_co8312173.1_g000001 Rmu_co8440105.1_g000001 Rmu_sc0006924.1_g000013
rosa_roxburghii Rroxscaffold_4G00283740
rosa_rugosa Rorug01G0380800
rosa_samantha Rh1AG391500 Rh1BG355500 Rh1CG368500 Rh1DG386200
rosa_wichuraiana Rw1G034410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1829
Acc36I ACCTGC 1 cut(s) 2837
AccB1I GGYRCC 1 cut(s) 1856
AccB7I CCANNNNNTGG 2 cut(s) 578, 2422
AccBSI CCGCTC 1 cut(s) 327
AccI GTMKAC 2 cut(s) 1110, 1530
AccII CGCG 6 cut(s) 1382, 1880, 1949, 2024, 2429, 2520
AclWI GGATC 8 cut(s) 128, 141, 1177, 1967, 1988, 2495, 2590, 2822
AcoI YGGCCR 5 cut(s) 1585, 1866, 1941, 2268, 2927
AcsI RAATTY 8 cut(s) 79, 288, 1199, 1222, 1653, 1723, 2095, 2419
AcuI CTGAAG 2 cut(s) 198, 1373
AcyI GRCGYC 2 cut(s) 1826, 1857
AfaI GTAC 7 cut(s) 274, 717, 1237, 1394, 2366, 2447, 2854
AflII CTTAAG 1 cut(s) 1609
AgeI ACCGGT 1 cut(s) 1778
AgsI TTSAA 8 cut(s) 53, 78, 151, 412, 463, 832, 2095, 2963
AhdI GACNNNNNGTC 1 cut(s) 2929
AjnI CCWGG 5 cut(s) 427, 571, 1574, 1680, 2188
AjuI GAANNNNNNNTTGG 4 cut(s) 431, 463, 1304, 1336
AleI CACNNNNGTG 1 cut(s) 2496
Alw21I GWGCWC 1 cut(s) 669
Alw26I GTCTC 4 cut(s) 244, 1742, 1765, 1991
AlwI GGATC 8 cut(s) 128, 141, 1177, 1967, 1988, 2495, 2590, 2822
AlwNI CAGNNNCTG 1 cut(s) 2343
Ama87I CYCGRG 1 cut(s) 524
AoxI GGCC 8 cut(s) 607, 1351, 1585, 1866, 1941, 2268, 2412, 2927
ApeKI GCWGC 6 cut(s) 1518, 1566, 1927, 2328, 2331, 2353
ApoI RAATTY 8 cut(s) 79, 288, 1199, 1222, 1653, 1723, 2095, 2419
AsiGI ACCGGT 1 cut(s) 1778
AspLEI GCGC 6 cut(s) 635, 1451, 1859, 1880, 1949, 2429
AsuC2I CCSGG 7 cut(s) 599, 775, 1708, 1837, 2005, 2164, 2842
AsuII TTCGAA 1 cut(s) 555
AvaI CYCGRG 1 cut(s) 524
AxyI CCTNAGG 1 cut(s) 1290
BaeGI GKGCMC 1 cut(s) 851
BalI TGGCCA 2 cut(s) 1587, 1868
BamHI GGATCC 1 cut(s) 133
BanI GGYRCC 1 cut(s) 1856
BanII GRGCYC 1 cut(s) 669
BarI GAAGNNNNNNTAC 2 cut(s) 1376, 1408
BauI CACGAG 3 cut(s) 1922, 2440, 2905
BbsI GAAGAC 3 cut(s) 451, 1785, 1943
Bbv12I GWGCWC 1 cut(s) 669
BbvCI CCTCAGC 1 cut(s) 1331
BbvI GCAGC 6 cut(s) 1505, 1578, 1939, 2315, 2318, 2340
BcgI CGANNNNNNTGC 6 cut(s) 664, 698, 1153, 1187, 2867, 2901
BciT130I CCWGG 5 cut(s) 429, 573, 1576, 1682, 2190
BciVI GTATCC 2 cut(s) 1024, 1879
BcnI CCSGG 7 cut(s) 599, 775, 1708, 1837, 2005, 2164, 2842
BcoDI GTCTC 4 cut(s) 244, 1742, 1765, 1991
BfaI CTAG 3 cut(s) 233, 245, 2991
BfmI CTRYAG 2 cut(s) 1460, 2029
BfoI RGCGCY 2 cut(s) 1452, 1860
BfrI CTTAAG 1 cut(s) 1609
BfuAI ACCTGC 1 cut(s) 2837
BfuI GTATCC 2 cut(s) 1024, 1879
BglI GCCNNNNNGGC 1 cut(s) 1865
BlpI GCTNAGC 2 cut(s) 965, 2589
BmeRI GACNNNNNGTC 1 cut(s) 2929
BmeT110I CYCGRG 1 cut(s) 524
BmrI ACTGGG 1 cut(s) 659
BmsI GCATC 5 cut(s) 65, 1266, 2175, 2865, 2943
BmuI ACTGGG 1 cut(s) 659
BpiI GAAGAC 3 cut(s) 451, 1785, 1943
BpmI CTGGAG 3 cut(s) 1766, 1920, 2138
Bpu10I CCTNAGC 3 cut(s) 789, 1331, 1616
Bpu1102I GCTNAGC 2 cut(s) 965, 2589
Bpu14I TTCGAA 1 cut(s) 555
BpuEI CTTGAG 7 cut(s) 113, 343, 428, 472, 483, 2454, 2510
BpuMI CCSGG 7 cut(s) 599, 775, 1708, 1837, 2005, 2164, 2842
BsaHI GRCGYC 2 cut(s) 1826, 1857
BsaI GGTCTC 2 cut(s) 1742, 1991
BsaWI WCCGGW 7 cut(s) 269, 712, 1493, 1634, 1778, 1796, 2503
Bse118I RCCGGY 6 cut(s) 1562, 1778, 1943, 2627, 2857, 2925
Bse1I ACTGG 4 cut(s) 185, 490, 654, 1230
Bse21I CCTNAGG 1 cut(s) 1290
Bse3DI GCAATG 3 cut(s) 936, 1381, 2643
BseBI CCWGG 5 cut(s) 429, 573, 1576, 1682, 2190
BseMI GCAATG 3 cut(s) 936, 1381, 2643
BseMII CTCAG 9 cut(s) 112, 394, 529, 780, 956, 1304, 1345, 1595, 1607
BseNI ACTGG 4 cut(s) 185, 490, 654, 1230
BseRI GAGGAG 2 cut(s) 1418, 2882
BseSI GKGCMC 1 cut(s) 851
BseX3I CGGCCG 2 cut(s) 1941, 2927
BseXI GCAGC 6 cut(s) 1505, 1578, 1939, 2315, 2318, 2340
BseYI CCCAGC 3 cut(s) 156, 375, 928
BsgI GTGCAG 2 cut(s) 69, 2329
Bsh1236I CGCG 6 cut(s) 1382, 1880, 1949, 2024, 2429, 2520
Bsh1285I CGRYCG 2 cut(s) 1944, 2930
BshFI GGCC 8 cut(s) 609, 1353, 1587, 1868, 1943, 2270, 2414, 2929
BshNI GGYRCC 1 cut(s) 1856
BshTI ACCGGT 1 cut(s) 1778
BsiEI CGRYCG 2 cut(s) 1944, 2930
BsiHKAI GWGCWC 1 cut(s) 669
BsiHKCI CYCGRG 1 cut(s) 524
BslFI GGGAC 6 cut(s) 1051, 1723, 2020, 2173, 2179, 2354
BsmAI GTCTC 4 cut(s) 244, 1742, 1765, 1991
BsmBI CGTCTC 1 cut(s) 1765
BsmFI GGGAC 6 cut(s) 1051, 1723, 2020, 2173, 2179, 2354
BsmI GAATGC 2 cut(s) 1569, 2842
BsnI GGCC 8 cut(s) 609, 1353, 1587, 1868, 1943, 2270, 2414, 2929
Bso31I GGTCTC 2 cut(s) 1742, 1991
BsoBI CYCGRG 1 cut(s) 524
Bsp119I TTCGAA 1 cut(s) 555
Bsp1286I GDGCHC 2 cut(s) 669, 851
Bsp1407I TGTACA 1 cut(s) 1235
Bsp1720I GCTNAGC 2 cut(s) 965, 2589
Bsp19I CCATGG 1 cut(s) 1646
BspANI GGCC 8 cut(s) 609, 1353, 1587, 1868, 1943, 2270, 2414, 2929
BspCNI CTCAG 9 cut(s) 111, 393, 528, 781, 957, 1303, 1344, 1596, 1608
BspFNI CGCG 6 cut(s) 1382, 1880, 1949, 2024, 2429, 2520
BspMAI CTGCAG 1 cut(s) 1464
BspMI ACCTGC 1 cut(s) 2837
BspPI GGATC 8 cut(s) 128, 141, 1177, 1967, 1988, 2495, 2590, 2822
BspT104I TTCGAA 1 cut(s) 555
BspT107I GGYRCC 1 cut(s) 1856
BspTI CTTAAG 1 cut(s) 1609
BspTNI GGTCTC 2 cut(s) 1742, 1991
BsrBI CCGCTC 1 cut(s) 327
BsrDI GCAATG 3 cut(s) 936, 1381, 2643
BsrFI RCCGGY 6 cut(s) 1562, 1778, 1943, 2627, 2857, 2925
BsrGI TGTACA 1 cut(s) 1235
BsrI ACTGG 4 cut(s) 185, 490, 654, 1230
BssAI RCCGGY 6 cut(s) 1562, 1778, 1943, 2627, 2857, 2925
BssNI GRCGYC 2 cut(s) 1826, 1857
BssSI CACGAG 3 cut(s) 1922, 2440, 2905
BssT1I CCWWGG 1 cut(s) 1646
Bst2BI CACGAG 3 cut(s) 1922, 2440, 2905
Bst2UI CCWGG 5 cut(s) 429, 573, 1576, 1682, 2190
Bst4CI ACNGT 4 cut(s) 2067, 2170, 2498, 2552
Bst6I CTCTTC 7 cut(s) 32, 47, 833, 1123, 1193, 1994, 2678
BstACI GRCGYC 2 cut(s) 1826, 1857
BstAFI CTTAAG 1 cut(s) 1609
BstAUI TGTACA 1 cut(s) 1235
BstBI TTCGAA 1 cut(s) 555
BstC8I GCNNGC 4 cut(s) 1945, 2026, 2268, 2988
BstDSI CCRYGG 2 cut(s) 1646, 2066
BstFNI CGCG 6 cut(s) 1382, 1880, 1949, 2024, 2429, 2520
BstH2I RGCGCY 2 cut(s) 1452, 1860
BstHHI GCGC 6 cut(s) 635, 1451, 1859, 1880, 1949, 2429
BstMAI GTCTC 4 cut(s) 244, 1742, 1765, 1991
BstMCI CGRYCG 2 cut(s) 1944, 2930
BstNI CCWGG 5 cut(s) 429, 573, 1576, 1682, 2190
BstNSI RCATGY 1 cut(s) 2371
BstSFI CTRYAG 2 cut(s) 1460, 2029
BstSLI GKGCMC 1 cut(s) 851
BstUI CGCG 6 cut(s) 1382, 1880, 1949, 2024, 2429, 2520
BstV1I GCAGC 6 cut(s) 1505, 1578, 1939, 2315, 2318, 2340
BstV2I GAAGAC 3 cut(s) 451, 1785, 1943
BstX2I RGATCY 1 cut(s) 133
BstYI RGATCY 1 cut(s) 133
BstZI CGGCCG 2 cut(s) 1941, 2927
Bsu36I CCTNAGG 1 cut(s) 1290
BsuI GTATCC 2 cut(s) 1024, 1879
BsuRI GGCC 8 cut(s) 609, 1353, 1587, 1868, 1943, 2270, 2414, 2929
BtgI CCRYGG 2 cut(s) 1646, 2066
BtgZI GCGATG 1 cut(s) 2619
BtsI GCAGTG 1 cut(s) 1588
BtsIMutI CAGTG 3 cut(s) 106, 483, 1588
BveI ACCTGC 1 cut(s) 2837
Cac8I GCNNGC 4 cut(s) 1945, 2026, 2268, 2988
CaiI CAGNNNCTG 1 cut(s) 2343
CfoI GCGC 6 cut(s) 635, 1451, 1859, 1880, 1949, 2429
Cfr10I RCCGGY 6 cut(s) 1562, 1778, 1943, 2627, 2857, 2925
CpoI CGGWCCG 2 cut(s) 2063, 2923
CseI GACGC 1 cut(s) 1516
Csp6I GTAC 7 cut(s) 273, 716, 1236, 1393, 2365, 2446, 2853
CspAI ACCGGT 1 cut(s) 1778
CspCI CAANNNNNGTGG 2 cut(s) 523, 558
CspI CGGWCCG 2 cut(s) 2063, 2923
CviQI GTAC 7 cut(s) 273, 716, 1236, 1393, 2365, 2446, 2853
DinI GGCGCC 1 cut(s) 1858
DriI GACNNNNNGTC 1 cut(s) 2929
EaeI YGGCCR 5 cut(s) 1585, 1866, 1941, 2268, 2927
EagI CGGCCG 2 cut(s) 1941, 2927
Eam1104I CTCTTC 7 cut(s) 32, 47, 833, 1123, 1193, 1994, 2678
Eam1105I GACNNNNNGTC 1 cut(s) 2929
EarI CTCTTC 7 cut(s) 32, 47, 833, 1123, 1193, 1994, 2678
EciI GGCGGA 7 cut(s) 1462, 1898, 2120, 2195, 2269, 2588, 2936
Ecl136II GAGCTC 1 cut(s) 667
EclXI CGGCCG 2 cut(s) 1941, 2927
Eco130I CCWWGG 1 cut(s) 1646
Eco24I GRGCYC 1 cut(s) 669
Eco31I GGTCTC 2 cut(s) 1742, 1991
Eco52I CGGCCG 2 cut(s) 1941, 2927
Eco53kI GAGCTC 1 cut(s) 667
Eco57I CTGAAG 2 cut(s) 198, 1373
Eco81I CCTNAGG 1 cut(s) 1290
Eco88I CYCGRG 1 cut(s) 524
EcoICRI GAGCTC 1 cut(s) 667
EcoO109I RGGNCCY 1 cut(s) 1065
EcoRII CCWGG 5 cut(s) 427, 571, 1574, 1680, 2188
EcoT14I CCWWGG 1 cut(s) 1646
EcoT38I GRGCYC 1 cut(s) 669
EgeI GGCGCC 1 cut(s) 1858
EheI GGCGCC 1 cut(s) 1858
ErhI CCWWGG 1 cut(s) 1646
Esp3I CGTCTC 1 cut(s) 1765
FaqI GGGAC 6 cut(s) 1051, 1723, 2020, 2173, 2179, 2354
FauI CCCGC 1 cut(s) 432
FblI GTMKAC 2 cut(s) 1110, 1530
FriOI GRGCYC 1 cut(s) 669
FspBI CTAG 3 cut(s) 233, 245, 2991
GlaI GCGC 6 cut(s) 634, 1450, 1858, 1879, 1948, 2428
GsaI CCCAGC 3 cut(s) 160, 379, 932
GsuI CTGGAG 3 cut(s) 1766, 1920, 2138
HaeII RGCGCY 2 cut(s) 1452, 1860
HaeIII GGCC 8 cut(s) 609, 1353, 1587, 1868, 1943, 2270, 2414, 2929
HgaI GACGC 1 cut(s) 1516
HhaI GCGC 6 cut(s) 635, 1451, 1859, 1880, 1949, 2429
Hin1I GRCGYC 2 cut(s) 1826, 1857
Hin6I GCGC 6 cut(s) 633, 1449, 1857, 1878, 1947, 2427
HinP1I GCGC 6 cut(s) 633, 1449, 1857, 1878, 1947, 2427
HincII GTYRAC 2 cut(s) 1111, 1776
HindII GTYRAC 2 cut(s) 1111, 1776
HindIII AAGCTT 2 cut(s) 1385, 1598
HinfI GANTC 8 cut(s) 207, 310, 384, 513, 1150, 1251, 2609, 2914
HpaI GTTAAC 1 cut(s) 1776
Hpy166II GTNNAC 4 cut(s) 1111, 1531, 1776, 2515
Hpy8I GTNNAC 4 cut(s) 1111, 1531, 1776, 2515
Hpy99I CGWCG 6 cut(s) 1112, 1828, 1831, 2003, 2040, 2253
HpyAV CCTTC 9 cut(s) 99, 173, 551, 1049, 1330, 1677, 1826, 2092, 2227
HpyCH4III ACNGT 4 cut(s) 2067, 2170, 2498, 2552
HpyCH4IV ACGT 3 cut(s) 1758, 1826, 2292
HpySE526I ACGT 3 cut(s) 1758, 1826, 2292
Hsp92I GRCGYC 2 cut(s) 1826, 1857
HspAI GCGC 6 cut(s) 633, 1449, 1857, 1878, 1947, 2427
KasI GGCGCC 1 cut(s) 1856
KflI GGGWCCC 1 cut(s) 1065
KroI GCCGGC 1 cut(s) 1943
KroNI GCCGGC 1 cut(s) 1945
KspAI GTTAAC 1 cut(s) 1776
LmnI GCTCC 4 cut(s) 601, 1091, 1307, 1901
Lsp1109I GCAGC 6 cut(s) 1505, 1578, 1939, 2315, 2318, 2340
LweI GCATC 5 cut(s) 65, 1266, 2175, 2865, 2943
MaeI CTAG 3 cut(s) 233, 245, 2991
MaeII ACGT 3 cut(s) 1758, 1826, 2292
MaeIII GTNAC 6 cut(s) 777, 1919, 2437, 2534, 2552, 2687
MbiI CCGCTC 1 cut(s) 327
MfeI CAATTG 1 cut(s) 719
MflI RGATCY 1 cut(s) 133
MhlI GDGCHC 2 cut(s) 669, 851
MlsI TGGCCA 2 cut(s) 1587, 1868
MluNI TGGCCA 2 cut(s) 1587, 1868
Mly113I GGCGCC 1 cut(s) 1857
MlyI GAGTC 3 cut(s) 507, 1144, 2618
MmeI TCCRAC 6 cut(s) 391, 535, 1146, 1847, 2018, 2272
Mox20I TGGCCA 2 cut(s) 1587, 1868
MroNI GCCGGC 1 cut(s) 1943
MscI TGGCCA 2 cut(s) 1587, 1868
MslI CAYNNNNRTG 4 cut(s) 674, 2207, 2496, 2618
Msp20I TGGCCA 2 cut(s) 1587, 1868
MspA1I CMGCKG 1 cut(s) 284
MspCI CTTAAG 1 cut(s) 1609
MunI CAATTG 1 cut(s) 719
Mva1269I GAATGC 2 cut(s) 1569, 2842
MvaI CCWGG 5 cut(s) 429, 573, 1576, 1682, 2190
MvnI CGCG 6 cut(s) 1382, 1880, 1949, 2024, 2429, 2520
NaeI GCCGGC 1 cut(s) 1945
NarI GGCGCC 1 cut(s) 1857
NciI CCSGG 7 cut(s) 599, 775, 1708, 1837, 2005, 2164, 2842
NcoI CCATGG 1 cut(s) 1646
NgoMIV GCCGGC 1 cut(s) 1943
NmeAIII GCCGAG 4 cut(s) 1062, 1350, 2296, 2556
NmuCI GTSAC 5 cut(s) 777, 1919, 2437, 2552, 2687
NspI RCATGY 1 cut(s) 2371
NspV TTCGAA 1 cut(s) 555
OliI CACNNNNGTG 1 cut(s) 2496
PaeR7I CTCGAG 1 cut(s) 524
PasI CCCWGGG 1 cut(s) 1575
PcsI WCGNNNNNNNCGW 6 cut(s) 1107, 1946, 1989, 2517, 2747, 2817
PctI GAATGC 2 cut(s) 1569, 2842
PdiI GCCGGC 1 cut(s) 1945
PfeI GAWTC 5 cut(s) 207, 310, 384, 1251, 2914
PflMI CCANNNNNTGG 2 cut(s) 578, 2422
PfoI TCCNGGA 3 cut(s) 597, 1706, 1835
PinAI ACCGGT 1 cut(s) 1778
PleI GAGTC 3 cut(s) 507, 1144, 2617
PluTI GGCGCC 1 cut(s) 1860
PpsI GAGTC 3 cut(s) 507, 1144, 2617
PpuMI RGGWCCY 1 cut(s) 1065
Psp124BI GAGCTC 1 cut(s) 669
Psp5II RGGWCCY 1 cut(s) 1065
Psp6I CCWGG 5 cut(s) 427, 571, 1574, 1680, 2188
PspFI CCCAGC 3 cut(s) 156, 375, 928
PspGI CCWGG 5 cut(s) 427, 571, 1574, 1680, 2188
PspPPI RGGWCCY 1 cut(s) 1065
PstI CTGCAG 1 cut(s) 1464
PstNI CAGNNNCTG 1 cut(s) 2343
PsuI RGATCY 1 cut(s) 133
PvuII CAGCTG 1 cut(s) 284
RsaI GTAC 7 cut(s) 274, 717, 1237, 1394, 2366, 2447, 2854
RsaNI GTAC 7 cut(s) 273, 716, 1236, 1393, 2365, 2446, 2853
RseI CAYNNNNRTG 4 cut(s) 674, 2207, 2496, 2618
Rsr2I CGGWCCG 2 cut(s) 2063, 2923
RsrII CGGWCCG 2 cut(s) 2063, 2923
SacI GAGCTC 1 cut(s) 669
SalI GTCGAC 1 cut(s) 1109
SchI GAGTC 3 cut(s) 507, 1144, 2618
SduI GDGCHC 2 cut(s) 669, 851
SfaNI GCATC 5 cut(s) 65, 1266, 2175, 2865, 2943
SfcI CTRYAG 2 cut(s) 1460, 2029
SfoI GGCGCC 1 cut(s) 1858
Sfr274I CTCGAG 1 cut(s) 524
SfuI TTCGAA 1 cut(s) 555
SgrAI CRCCGGYG 1 cut(s) 2857
SlaI CTCGAG 1 cut(s) 524
SmiMI CAYNNNNRTG 4 cut(s) 674, 2207, 2496, 2618
SmlI CTYRAG 9 cut(s) 128, 322, 443, 451, 498, 524, 1609, 2469, 2525
SmoI CTYRAG 9 cut(s) 128, 322, 443, 451, 498, 524, 1609, 2469, 2525
SspDI GGCGCC 1 cut(s) 1856
SspI AATATT 2 cut(s) 565, 586
SspMI CTAG 3 cut(s) 233, 245, 2991
SstI GAGCTC 1 cut(s) 669
StyI CCWWGG 1 cut(s) 1646
TaaI ACNGT 4 cut(s) 2067, 2170, 2498, 2552
TaiI ACGT 3 cut(s) 1761, 1829, 2295
TaqII GACCGA 1 cut(s) 1973
TatI WGTACW 2 cut(s) 1235, 2364
TauI GCSGC 6 cut(s) 935, 1382, 1857, 1883, 1970, 1973
TfiI GAWTC 5 cut(s) 207, 310, 384, 1251, 2914
TscAI CASTG 3 cut(s) 106, 490, 1595
TseFI GTSAC 5 cut(s) 777, 1919, 2437, 2552, 2687
TseI GCWGC 6 cut(s) 1518, 1566, 1927, 2328, 2331, 2353
Tsp45I GTSAC 5 cut(s) 777, 1919, 2437, 2552, 2687
TspDTI ATGAA 5 cut(s) 17, 186, 989, 1501, 1808
TspGWI ACGGA 1 cut(s) 1779
TspRI CASTG 3 cut(s) 106, 490, 1595
Van91I CCANNNNNTGG 2 cut(s) 578, 2422
Vha464I CTTAAG 1 cut(s) 1609
XapI RAATTY 8 cut(s) 79, 288, 1199, 1222, 1653, 1723, 2095, 2419
XbaI TCTAGA 1 cut(s) 244
XceI RCATGY 1 cut(s) 2371
XcmI CCANNNNNNNNNTGG 4 cut(s) 1440, 1678, 2380, 2941
XhoI CTCGAG 1 cut(s) 524
XmiI GTMKAC 2 cut(s) 1110, 1530
XspI CTAG 3 cut(s) 233, 245, 2991
ZraI GACGTC 1 cut(s) 1827
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.