Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0012250

Physical Location & Seq

Genomic Coordinates Forward (+)

28058104 .. 28062751

Gene Structure 4648 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1181700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3108 bp

ATGAAAGTACCTTCCTTTTCCTCCTCCTCCTTCCCTCTCTCTCTCCTCTTCTTCTTCTCTCTCCAAACCTCCATAACTCTCTGCTCTTCTTCCTCTAATTCTTCTGTGTCCTTCCAGCTCATTGCTCTGCTCTCCCTCAAATCCTCCCTCAAAGACCCTCTCTCCTCCTTCCACGATTGGGTTTCCCCCAATTCCCACCCAAACGGCGGCGTCTCCGGTTGGTGTTCGTGGTCCGGCGTCAAATGCCAACCCGACACCTCCCAAGTCGTTTCCCTCGACCTCTCCCGCCGCAACCTCTCCGGCCAAATCCCACCACAAATCCGCCACCTTTCTTCCCTAATCCACCTCAATTTGAGCGGCAACGCCTTCAACGGCCCTCTCCAGCCCGCCATGTTCGAGCTCGACAACCTCAGGATCCTCGACATCAGCCACAACGACTTCAAGTCGGATTTCCCGCCCGGGATTTCGAAGCTCAGGTTTTTAAGAGTCTTTACCGCTTACAGTAACAGCTTCACTGGCCCATTGCCTCAAGAATTCGTCAAGCTCCGCTACCTCGAGCAGCTCAACCTCGGTGGAAGTTACTTCGATGGTAAAATCCCAGAAGGGTACGGAACATTCCCCAAGTTAAAATTGCTCTACCTCGCCGGAAATGCACTCAACGGTTCAATTCCGCCGCAATTGGGTTCCTTATCGGAGCTGACTCGGATGGAGATCGGGTACAACGAGTATTCAGGTGGGTTTCCTATAGAACTCGGAATGCTGTCAAATCTCACGTACTTGGACGTCTCCAACGCCTTTCTCTCCGGCGCTCTCCCGCCGGAGCTTGGCAACTTAACCATGCTTGACACATTGCTGCTCTTCAAGAACCGATTTTCAGGCTTTATCCCAGCAACTATCGGCCTCTTACAGAGACTGAAGACACTCGACTTGTCGGATAATCAGCTTTCCGGCTCAATCCCGCCGGGAATTGGTACATTGAAAGAGCTGACCATGATAAGCTTGATGAACAACTTTCTCGTCGGAGAAATCCCGGAAAAAATCGGAGAGCTGCCCAACTTAGAAAACCTCCTCCTCTGGAACAATTCGCTGACGGGTCTCCTTCCCCAGGGCTTAGGATTCAGCGGGAAGCTCCTGCGAGTCGATGTCTCCTCCAATTCTCTCACCGGCCCGATCCCGCCAAATCTCTGCGACGGCAACAAGCTCCAAAAGCTCATACTTTTCTCCAACCGGTTCACCGACCCTCTTCCCAAAACCTTAGCAAACTGCACTTCTCTGTTCAGACTCCGAATCCAGAACAACCAAATCAACGGGTCGGTTCCGACGGGTTTCGGGTTATTACCCAATCTAACCTACGTCGATTTCAGCGGCAACAATTTCACAGGCACGATCCCGGAAGATCTCGGCAATGCCGAAGCTCTCAGTTACTTGAACATTTCGCTGAACCCATTGCACTCAGTCCTTCCGTCAAACATCTGGCGCGCACCAAATCTGCAGATCTTCTCTGCTAGCTCCGGCCACCTCACAGGAAAAATCCCCGACTTCATCGGCTGCCAAAGCCTGTACAAGATCGAACTTCAACGTAACGACTTTAACGGCTCGATCCCATGGGACATCGGGCACTGCGAGAAGCTTCTCAGCTTGAATCTAAGCCGCAATTCTCTCACCGGCATTATCCCATGGGAAATCTCCGCTTTGCCCTCCATCACCGACCTCGACCTCTCCCGGAACTTTCTCTCCGGCACGATCCCGTCCAACTTCGAGAACTGTAGCACGTTGGAGAGCTTCAACGTTTCGTTTAATTTGTTGACCGGTCCGATCCCCGCGTCCGGTTCCATTTTCCCGAATCTCCATCCGTCGTCGTTTTTGGGGAATAAAGGATTATGTGGCGGCGTTTTGGGGAAGCCCTGTGACACCTTGTCGGCCGGAGCCATGGAGGTTCGCGGCCACGAGCAGCCGAAGAAGACGGCGGGCGCGATAGTGTGGATCATGGCGGCTGCTTTCGGTATCGGGCTTTTTGTTCTCATCGCTGGCACCCGCTGCTTCCGCGCGAATTACAGCCGCCGGATGGACGAGAGTCAGCAGATTGGACCGTGGAAATTAACCGCTTTCCAGCGGTTAAATTTCACGGCGGATGATGTGTTGGAGTGTTTGGAGATGAGCGATAAGATTATAGGGATGGGGTCCACGGGGACGGTTTATAGGGCGGAAATGCCAGGTGGCGAGTTCATAGCCGTTAAGAAGCTGTGGGGAAAACAGAAGGAGAACAATAGTGATCTGATCCGACGGCGGAGAGGGGTCCTGGCTGAGGTGGACGTGTTGGGGAACGTGAGGCACAGGAATATAGTAAGGTTGTTAGGGTGCTGCTGCAACAGAGACATCACTATGCTGCTCTACGAGTACATGCCCAACGGGAATTTGGATGATTTGTTGCATGGCAAAAACAAGGCGCAGAATTTGGGTGCGGATTGGGTCACGAGGTACAAGATCGCCCTCGGCGTGGCGCAGGGGATTTGTTACCTGCACCACGACTGTAACCCCGTGATCGTGCACCGTGATCTTAAGCCGAGTAATATCTTGTTGGATAGTGAGATGGAGGCCAGAGTGGCGGATTTTGGGGTGGCTAAGTTGATCCAAAGCGATGAGTCCATGTCCGTCATTGCCGGCTCCTATGGCTACATTGCTCCAGAATATGCTTATACCCTACAAGTGGATGAAAAGAGTGACATATACAGTTATGGTGTGGTGTTGATGGAGATTTTAAGCGGGAAAAGATCGGTGGACGCCGAGTTCGGAGATGGGAACAGCATAGTGGATTGGGTTAGGTCGAAGATCAAGACGAAGGACGGGATCAACGATATTTTGGACAAGAATGCCGGGGCGGGTTGTGCGCCGGTGAGGGAGGAGATGATGCAGATGCTTAGGATTGCTTTGCTCTGCACGAGCCGGAACCCGGCGGACCGGCCGTCGATGAGGGACGTGGTGTTGATGCTGCTTGAGGCCAAGCCCAAGAGGAAGGTCCTAGGCAATGTTGGGCATGGTCACAATGGGAGTGGTTGTGCTGAATATCATGAGATTCCCTTGCCTCAGAAGCCGTCGGTGGAATGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001763 GO:0003006 GO:0005575 GO:0005623 GO:0005886 GO:0007275 GO:0008150 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009987 GO:0010016 GO:0010065 GO:0010067 GO:0010087 GO:0010089 GO:0010154 GO:0010223 GO:0010346 GO:0016020 GO:0022414 GO:0032501 GO:0032502 GO:0044464 GO:0048316 GO:0048367 GO:0048507 GO:0048508 GO:0048608 GO:0048646 GO:0048731 GO:0048856 GO:0051301 GO:0061458 GO:0071944 GO:1905393

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase

Protein Analysis

1036

Amino Acids

113.26

Weight (kDa)

6.67

Isoelectric Point (pI)

33.37

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRR_14	PF23598	134 - 241	9.8e-06	Leucine-rich repeat region
LRR_14	PF23598	250 - 356	9.3e-09	Leucine-rich repeat region
LRR_8	PF13855	519 - 576	4.9e-07	Leucine rich repeat
Pkinase	PF00069	721 - 994	1.9e-45	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	721 - 995	2.3e-39	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0012250)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G61480
fragaria_vesca	FvH4_7g27270
malus_domestica	MD01G1181700.v1.1 MD07G1251900.v1.1
prunus_persica	Prupe.2G275500_v2.0.a1
pyrus_communis	pycom01g19150 pycom07g22430
rosa_chinensis	RchiOBHm_Chr1g0374101
rosa_laevigata	RLG00000026763
rosa_multiflora	Rmu_co8312173.1_g000001 Rmu_co8440105.1_g000001 Rmu_sc0006924.1_g000013
rosa_roxburghii	Rroxscaffold_4G00283740
rosa_rugosa	Rorug01G0380800
rosa_samantha	Rh1AG391500 Rh1BG355500 Rh1CG368500 Rh1DG386200
rosa_wichuraiana	Rw1G034410

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	786
Acc36I	ACCTGC	1 cut(s)	2526
AccB1I	GGYRCC	1 cut(s)	2030
AccBSI	CCGCTC	1 cut(s)	357
AccII	CGCG	6 cut(s)	1479, 1823, 1941, 1973, 2046, 2048
AclI	AACGTT	1 cut(s)	1788
AcoI	YGGCCR	5 cut(s)	301, 1513, 1920, 1942, 2960
AcsI	RAATTY	4 cut(s)	533, 2119, 2413, 2452
AcuI	CTGAAG	1 cut(s)	935
AcyI	GRCGYC	4 cut(s)	210, 237, 783, 2781
AfaI	GTAC	8 cut(s)	9, 608, 719, 776, 973, 1562, 2399, 2480
AflII	CTTAAG	1 cut(s)	2558
AflIII	ACRYGT	1 cut(s)	2313
AgeI	ACCGGT	2 cut(s)	1227, 1808
AgsI	TTSAA	9 cut(s)	370, 442, 666, 862, 979, 1429, 1577, 1642, 1786
AhdI	GACNNNNNGTC	3 cut(s)	442, 1915, 2962
AjiI	CACGTC	2 cut(s)	2314, 2977
AjnI	CCWGG	3 cut(s)	1104, 2212, 2298
AloI	GAACNNNNNNTCC	4 cut(s)	1718, 1750, 2771, 2803
Alw21I	GWGCWC	2 cut(s)	402, 2550
Alw26I	GTCTC	6 cut(s)	217, 790, 904, 1100, 1150, 2367
Alw44I	GTGCAC	1 cut(s)	2546
AlwNI	CAGNNNCTG	1 cut(s)	913
Ama87I	CYCGRG	2 cut(s)	458, 554
ApaLI	GTGCAC	1 cut(s)	2546
ApoI	RAATTY	4 cut(s)	533, 2119, 2413, 2452
ArsI	GACNNNNNNTTYG	2 cut(s)	2771, 2803
AsiGI	ACCGGT	2 cut(s)	1227, 1808
AspA2I	CCTAGG	1 cut(s)	3019
AspLEI	GCGC	8 cut(s)	809, 1479, 1481, 1973, 2048, 2449, 2503, 2890
AsuC2I	CCSGG	8 cut(s)	459, 460, 963, 1031, 1391, 1723, 2875, 2951
AsuHPI	GGTGA	5 cut(s)	1153, 1225, 1654, 1696, 2905
AsuII	TTCGAA	1 cut(s)	467
AsuNHI	GCTAGC	1 cut(s)	1505
AvaI	CYCGRG	2 cut(s)	458, 554
AvaII	GGWCC	7 cut(s)	231, 1811, 2087, 2181, 2296, 2956, 3016
AvrII	CCTAGG	1 cut(s)	3019
AxyI	CCTNAGG	1 cut(s)	410
BaeGI	GKGCMC	2 cut(s)	1620, 2550
BamHI	GGATCC	1 cut(s)	414
BanI	GGYRCC	1 cut(s)	2030
BanII	GRGCYC	1 cut(s)	402
BauI	CACGAG	3 cut(s)	1946, 2473, 2938
BbsI	GAAGAC	2 cut(s)	923, 1967
Bbv12I	GWGCWC	2 cut(s)	402, 2550
BbvCI	CCTCAGC	1 cut(s)	2304
BccI	CCATC	9 cut(s)	581, 700, 1709, 1857, 2059, 2170, 2584, 2743, 2789
BciT130I	CCWGG	3 cut(s)	1106, 2214, 2300
BcnI	CCSGG	8 cut(s)	459, 460, 963, 1031, 1391, 1723, 2875, 2951
BcoDI	GTCTC	6 cut(s)	217, 790, 904, 1100, 1150, 2367
BfaI	CTAG	2 cut(s)	1506, 3020
BfmI	CTRYAG	3 cut(s)	744, 1490, 1765
BfoI	RGCGCY	1 cut(s)	810
BfrI	CTTAAG	1 cut(s)	2558
BfuAI	ACCTGC	1 cut(s)	2526
BglI	GCCNNNNNGGC	1 cut(s)	2603
BglII	AGATCT	2 cut(s)	1396, 1494
BlnI	CCTAGG	1 cut(s)	3019
Bme18I	GGWCC	7 cut(s)	231, 1811, 2087, 2181, 2296, 2956, 3016
BmeRI	GACNNNNNGTC	3 cut(s)	442, 1915, 2962
BmeT110I	CYCGRG	2 cut(s)	458, 554
BmgBI	CACGTC	2 cut(s)	2314, 2977
BmsI	GCATC	3 cut(s)	2898, 2904, 2976
BmtI	GCTAGC	1 cut(s)	1509
BoxI	GACNNNNGTC	1 cut(s)	2073
BpiI	GAAGAC	2 cut(s)	923, 1967
BpmI	CTGGAG	2 cut(s)	365, 2667
Bpu10I	CCTNAGC	5 cut(s)	473, 1111, 1255, 2304, 2918
Bpu14I	TTCGAA	1 cut(s)	467
BpuEI	CTTGAG	2 cut(s)	513, 3014
BpuMI	CCSGG	8 cut(s)	459, 460, 963, 1031, 1391, 1723, 2875, 2951
BsaAI	YACGTR	1 cut(s)	774
BsaBI	GATNNNNATC	1 cut(s)	710
BsaHI	GRCGYC	4 cut(s)	210, 237, 783, 2781
BsaI	GGTCTC	1 cut(s)	1100
BsaWI	WCCGGW	4 cut(s)	215, 1227, 1808, 1826
BsaXI	ACNNNNNCTCC	8 cut(s)	146, 176, 701, 731, 1718, 1748, 3040, 3070
Bse118I	RCCGGY	7 cut(s)	1163, 1227, 1664, 1808, 2660, 2890, 2958
Bse1I	ACTGG	1 cut(s)	520
Bse21I	CCTNAGG	1 cut(s)	410
Bse3DI	GCAATG	8 cut(s)	120, 521, 848, 1411, 1445, 2655, 2676, 3031
Bse8I	GATNNNNATC	1 cut(s)	710
BseBI	CCWGG	3 cut(s)	1106, 2214, 2300
BseGI	GGATG	7 cut(s)	711, 1849, 2070, 2137, 2181, 2425, 2716
BseJI	GATNNNNATC	1 cut(s)	710
BseMI	GCAATG	8 cut(s)	120, 521, 848, 1411, 1445, 2655, 2676, 3031
BseMII	CTCAG	7 cut(s)	424, 487, 1432, 1467, 1648, 2295, 3098
BseNI	ACTGG	1 cut(s)	520
BsePI	GCGCGC	1 cut(s)	1477
BseRI	GAGGAG	8 cut(s)	13, 16, 35, 154, 1058, 1061, 1138, 2915
BseSI	GKGCMC	2 cut(s)	1620, 2550
BseX3I	CGGCCG	2 cut(s)	1920, 2960
BseYI	CCCAGC	1 cut(s)	886
BsgI	GTGCAG	3 cut(s)	1249, 2504, 2920
Bsh1236I	CGCG	6 cut(s)	1479, 1823, 1941, 1973, 2046, 2048
Bsh1285I	CGRYCG	2 cut(s)	1923, 2963
BshNI	GGYRCC	1 cut(s)	2030
BshTI	ACCGGT	2 cut(s)	1227, 1808
BsiEI	CGRYCG	2 cut(s)	1923, 2963
BsiHKAI	GWGCWC	2 cut(s)	402, 2550
BsiHKCI	CYCGRG	2 cut(s)	458, 554
BslFI	GGGAC	3 cut(s)	1622, 2203, 2987
BsmAI	GTCTC	6 cut(s)	217, 790, 904, 1100, 1150, 2367
BsmBI	CGTCTC	2 cut(s)	217, 790
BsmFI	GGGAC	3 cut(s)	1622, 2203, 2987
BsmI	GAATGC	2 cut(s)	762, 2875
Bso31I	GGTCTC	1 cut(s)	1100
BsoBI	CYCGRG	2 cut(s)	458, 554
Bsp119I	TTCGAA	1 cut(s)	467
Bsp1286I	GDGCHC	3 cut(s)	402, 1620, 2550
Bsp1407I	TGTACA	1 cut(s)	1560
Bsp19I	CCATGG	3 cut(s)	1604, 1676, 1929
BspCNI	CTCAG	7 cut(s)	423, 486, 1431, 1466, 1647, 2296, 3097
BspFNI	CGCG	6 cut(s)	1479, 1823, 1941, 1973, 2046, 2048
BspHI	TCATGA	1 cut(s)	3067
BspMAI	CTGCAG	1 cut(s)	1494
BspMI	ACCTGC	1 cut(s)	2526
BspOI	GCTAGC	1 cut(s)	1509
BspQI	GCTCTTC	2 cut(s)	91, 863
BspT104I	TTCGAA	1 cut(s)	467
BspT107I	GGYRCC	1 cut(s)	2030
BspTI	CTTAAG	1 cut(s)	2558
BspTNI	GGTCTC	1 cut(s)	1100
BsrBI	CCGCTC	1 cut(s)	357
BsrDI	GCAATG	8 cut(s)	120, 521, 848, 1411, 1445, 2655, 2676, 3031
BsrFI	RCCGGY	7 cut(s)	1163, 1227, 1664, 1808, 2660, 2890, 2958
BsrGI	TGTACA	1 cut(s)	1560
BsrI	ACTGG	1 cut(s)	520
BssAI	RCCGGY	7 cut(s)	1163, 1227, 1664, 1808, 2660, 2890, 2958
BssHII	GCGCGC	1 cut(s)	1477
BssNI	GRCGYC	4 cut(s)	210, 237, 783, 2781
BssSI	CACGAG	3 cut(s)	1946, 2473, 2938
BssT1I	CCWWGG	4 cut(s)	1604, 1676, 1929, 3019
Bst2BI	CACGAG	3 cut(s)	1946, 2473, 2938
Bst2UI	CCWGG	3 cut(s)	1106, 2214, 2300
Bst4CI	ACNGT	8 cut(s)	503, 662, 1766, 2091, 2194, 2531, 2552, 2732
Bst6I	CTCTTC	4 cut(s)	53, 91, 863, 1248
BstACI	GRCGYC	4 cut(s)	210, 237, 783, 2781
BstAFI	CTTAAG	1 cut(s)	2558
BstAPI	GCANNNNNTGC	1 cut(s)	2037
BstAUI	TGTACA	1 cut(s)	1560
BstBAI	YACGTR	1 cut(s)	774
BstBI	TTCGAA	1 cut(s)	467
BstC8I	GCNNGC	6 cut(s)	387, 1479, 1507, 1969, 2029, 2662
BstDSI	CCRYGG	5 cut(s)	1604, 1676, 1929, 2090, 2184
BstENI	CCTNNNNNAGG	2 cut(s)	1103, 2303
BstF5I	GGATG	7 cut(s)	711, 1849, 2070, 2137, 2181, 2425, 2716
BstFNI	CGCG	6 cut(s)	1479, 1823, 1941, 1973, 2046, 2048
BstH2I	RGCGCY	1 cut(s)	810
BstHHI	GCGC	8 cut(s)	809, 1479, 1481, 1973, 2048, 2449, 2503, 2890
BstMAI	GTCTC	6 cut(s)	217, 790, 904, 1100, 1150, 2367
BstMCI	CGRYCG	2 cut(s)	1923, 2963
BstNI	CCWGG	3 cut(s)	1106, 2214, 2300
BstNSI	RCATGY	1 cut(s)	2404
BstPAI	GACNNNNGTC	1 cut(s)	2073
BstSFI	CTRYAG	3 cut(s)	744, 1490, 1765
BstSLI	GKGCMC	2 cut(s)	1620, 2550
BstUI	CGCG	6 cut(s)	1479, 1823, 1941, 1973, 2046, 2048
BstV2I	GAAGAC	2 cut(s)	923, 1967
BstX2I	RGATCY	3 cut(s)	414, 1396, 1494
BstYI	RGATCY	3 cut(s)	414, 1396, 1494
BstZI	CGGCCG	2 cut(s)	1920, 2960
Bsu36I	CCTNAGG	1 cut(s)	410
BtgI	CCRYGG	5 cut(s)	1604, 1676, 1929, 2090, 2184
BtgZI	GCGATG	2 cut(s)	2008, 2652
BtrI	CACGTC	2 cut(s)	2314, 2977
BtsCI	GGATG	7 cut(s)	711, 1849, 2070, 2137, 2181, 2425, 2716
BtsI	GCAGTG	1 cut(s)	1618
BtsIMutI	CAGTG	2 cut(s)	513, 1618
BveI	ACCTGC	1 cut(s)	2526
Cac8I	GCNNGC	6 cut(s)	387, 1479, 1507, 1969, 2029, 2662
CaiI	CAGNNNCTG	1 cut(s)	913
CciI	TCATGA	1 cut(s)	3067
CfoI	GCGC	8 cut(s)	809, 1479, 1481, 1973, 2048, 2449, 2503, 2890
Cfr10I	RCCGGY	7 cut(s)	1163, 1227, 1664, 1808, 2660, 2890, 2958
Cfr9I	CCCGGG	1 cut(s)	458
CpoI	CGGWCCG	2 cut(s)	1811, 2956
CseI	GACGC	4 cut(s)	199, 226, 1812, 2789
Csp6I	GTAC	8 cut(s)	8, 607, 718, 775, 972, 1561, 2398, 2479
CspAI	ACCGGT	2 cut(s)	1227, 1808
CspCI	CAANNNNNGTGG	4 cut(s)	553, 588, 3031, 3066
CspI	CGGWCCG	2 cut(s)	1811, 2956
CviQI	GTAC	8 cut(s)	8, 607, 718, 775, 972, 1561, 2398, 2479
DriI	GACNNNNNGTC	3 cut(s)	442, 1915, 2962
EaeI	YGGCCR	5 cut(s)	301, 1513, 1920, 1942, 2960
EagI	CGGCCG	2 cut(s)	1920, 2960
Eam1104I	CTCTTC	4 cut(s)	53, 91, 863, 1248
Eam1105I	GACNNNNNGTC	3 cut(s)	442, 1915, 2962
EarI	CTCTTC	4 cut(s)	53, 91, 863, 1248
EciI	GGCGGA	7 cut(s)	311, 660, 2144, 2219, 2302, 2621, 2969
Ecl136II	GAGCTC	1 cut(s)	400
EclXI	CGGCCG	2 cut(s)	1920, 2960
Eco130I	CCWWGG	4 cut(s)	1604, 1676, 1929, 3019
Eco24I	GRGCYC	1 cut(s)	402
Eco31I	GGTCTC	1 cut(s)	1100
Eco47I	GGWCC	7 cut(s)	231, 1811, 2087, 2181, 2296, 2956, 3016
Eco52I	CGGCCG	2 cut(s)	1920, 2960
Eco53kI	GAGCTC	1 cut(s)	400
Eco57I	CTGAAG	1 cut(s)	935
Eco81I	CCTNAGG	1 cut(s)	410
Eco88I	CYCGRG	2 cut(s)	458, 554
EcoICRI	GAGCTC	1 cut(s)	400
EcoNI	CCTNNNNNAGG	2 cut(s)	1103, 2303
EcoO109I	RGGNCCY	2 cut(s)	2296, 3016
EcoRI	GAATTC	1 cut(s)	533
EcoRII	CCWGG	3 cut(s)	1104, 2212, 2298
EcoT14I	CCWWGG	4 cut(s)	1604, 1676, 1929, 3019
EcoT38I	GRGCYC	1 cut(s)	402
ErhI	CCWWGG	4 cut(s)	1604, 1676, 1929, 3019
Esp3I	CGTCTC	2 cut(s)	217, 790
FaqI	GGGAC	3 cut(s)	1622, 2203, 2987
FokI	GGATG	7 cut(s)	718, 1836, 2077, 2144, 2188, 2432, 2723
FriOI	GRGCYC	1 cut(s)	402
FspBI	CTAG	2 cut(s)	1506, 3020
GlaI	GCGC	8 cut(s)	808, 1478, 1480, 1972, 2047, 2448, 2502, 2889
GsaI	CCCAGC	1 cut(s)	890
GsuI	CTGGAG	2 cut(s)	365, 2667
HaeII	RGCGCY	1 cut(s)	810
HgaI	GACGC	4 cut(s)	199, 226, 1812, 2789
HhaI	GCGC	8 cut(s)	809, 1479, 1481, 1973, 2048, 2449, 2503, 2890
Hin1I	GRCGYC	4 cut(s)	210, 237, 783, 2781
Hin6I	GCGC	8 cut(s)	807, 1477, 1479, 1971, 2046, 2447, 2501, 2888
HinP1I	GCGC	8 cut(s)	807, 1477, 1479, 1971, 2046, 2447, 2501, 2888
HincII	GTYRAC	1 cut(s)	1806
HindII	GTYRAC	1 cut(s)	1806
HindIII	AAGCTT	2 cut(s)	997, 1628
HphI	GGTGA	5 cut(s)	1153, 1225, 1654, 1696, 2905
Hpy166II	GTNNAC	6 cut(s)	1233, 1806, 2184, 2311, 2548, 2779
Hpy8I	GTNNAC	6 cut(s)	1233, 1806, 2184, 2311, 2548, 2779
Hpy99I	CGWCG	9 cut(s)	1022, 1193, 1324, 1358, 1858, 1861, 2286, 2968, 3097
HpyCH4III	ACNGT	8 cut(s)	503, 662, 1766, 2091, 2194, 2531, 2552, 2732
HpyCH4IV	ACGT	9 cut(s)	773, 783, 1353, 1579, 1772, 1788, 2313, 2325, 2976
HpySE526I	ACGT	9 cut(s)	773, 783, 1353, 1579, 1772, 1788, 2313, 2325, 2976
Hsp92I	GRCGYC	4 cut(s)	210, 237, 783, 2781
HspAI	GCGC	8 cut(s)	807, 1477, 1479, 1971, 2046, 2447, 2501, 2888
KroI	GCCGGC	1 cut(s)	2660
KroNI	GCCGGC	1 cut(s)	2662
LguI	GCTCTTC	2 cut(s)	91, 863
LmnI	GCTCC	9 cut(s)	549, 694, 820, 1134, 1206, 1514, 1925, 2669, 2686
LweI	GCATC	3 cut(s)	2898, 2904, 2976
MaeI	CTAG	2 cut(s)	1506, 3020
MaeII	ACGT	9 cut(s)	773, 783, 1353, 1579, 1772, 1788, 2313, 2325, 2976
MbiI	CCGCTC	1 cut(s)	357
MfeI	CAATTG	1 cut(s)	677
MflI	RGATCY	3 cut(s)	414, 1396, 1494
MhlI	GDGCHC	3 cut(s)	402, 1620, 2550
MlyI	GAGTC	6 cut(s)	495, 694, 1146, 1275, 2083, 2651
MroNI	GCCGGC	1 cut(s)	2660
MslI	CAYNNNNRTG	1 cut(s)	2381
MspA1I	CMGCKG	4 cut(s)	1122, 1365, 2037, 2113
MspCI	CTTAAG	1 cut(s)	2558
MunI	CAATTG	1 cut(s)	677
Mva1269I	GAATGC	2 cut(s)	762, 2875
MvaI	CCWGG	3 cut(s)	1106, 2214, 2300
MvnI	CGCG	6 cut(s)	1479, 1823, 1941, 1973, 2046, 2048
NaeI	GCCGGC	1 cut(s)	2662
NciI	CCSGG	8 cut(s)	459, 460, 963, 1031, 1391, 1723, 2875, 2951
NcoI	CCATGG	3 cut(s)	1604, 1676, 1929
NgoMIV	GCCGGC	1 cut(s)	2660
NheI	GCTAGC	1 cut(s)	1505
NmeAIII	GCCGAG	4 cut(s)	1380, 2472, 2589, 2809
NmuCI	GTSAC	4 cut(s)	1907, 2470, 2720, 3038
NspI	RCATGY	1 cut(s)	2404
NspV	TTCGAA	1 cut(s)	467
PaeR7I	CTCGAG	1 cut(s)	554
PagI	TCATGA	1 cut(s)	3067
PasI	CCCWGGG	1 cut(s)	1105
PauI	GCGCGC	1 cut(s)	1477
PciSI	GCTCTTC	2 cut(s)	91, 863
PcsI	WCGNNNNNNNCGW	7 cut(s)	273, 720, 1407, 1620, 1970, 2493, 2850
PctI	GAATGC	2 cut(s)	762, 2875
PdiI	GCCGGC	1 cut(s)	2662
PfeI	GAWTC	5 cut(s)	1116, 1287, 1642, 1843, 3073
PfoI	TCCNGGA	3 cut(s)	1029, 1389, 1721
PinAI	ACCGGT	2 cut(s)	1227, 1808
PleI	GAGTC	6 cut(s)	494, 694, 1145, 1275, 2082, 2650
PpsI	GAGTC	6 cut(s)	494, 694, 1145, 1275, 2082, 2650
Ppu21I	YACGTR	1 cut(s)	774
PpuMI	RGGWCCY	2 cut(s)	2296, 3016
PshAI	GACNNNNGTC	1 cut(s)	2073
Psp124BI	GAGCTC	1 cut(s)	402
Psp1406I	AACGTT	1 cut(s)	1788
Psp5II	RGGWCCY	2 cut(s)	2296, 3016
Psp6I	CCWGG	3 cut(s)	1104, 2212, 2298
PspFI	CCCAGC	1 cut(s)	886
PspGI	CCWGG	3 cut(s)	1104, 2212, 2298
PspPPI	RGGWCCY	2 cut(s)	2296, 3016
PspXI	VCTCGAGB	1 cut(s)	554
PstI	CTGCAG	1 cut(s)	1494
PstNI	CAGNNNCTG	1 cut(s)	913
PsuI	RGATCY	3 cut(s)	414, 1396, 1494
PteI	GCGCGC	1 cut(s)	1477
RsaI	GTAC	8 cut(s)	9, 608, 719, 776, 973, 1562, 2399, 2480
RsaNI	GTAC	8 cut(s)	8, 607, 718, 775, 972, 1561, 2398, 2479
RseI	CAYNNNNRTG	1 cut(s)	2381
Rsr2I	CGGWCCG	2 cut(s)	1811, 2956
RsrII	CGGWCCG	2 cut(s)	1811, 2956
SacI	GAGCTC	1 cut(s)	402
SapI	GCTCTTC	2 cut(s)	91, 863
SchI	GAGTC	6 cut(s)	495, 694, 1146, 1275, 2083, 2651
SduI	GDGCHC	3 cut(s)	402, 1620, 2550
SfaNI	GCATC	3 cut(s)	2898, 2904, 2976
SfcI	CTRYAG	3 cut(s)	744, 1490, 1765
Sfr274I	CTCGAG	1 cut(s)	554
SfuI	TTCGAA	1 cut(s)	467
SgrAI	CRCCGGYG	1 cut(s)	2890
SinI	GGWCC	7 cut(s)	231, 1811, 2087, 2181, 2296, 2956, 3016
SlaI	CTCGAG	1 cut(s)	554
SmaI	CCCGGG	1 cut(s)	460
SmiMI	CAYNNNNRTG	1 cut(s)	2381
SmlI	CTYRAG	4 cut(s)	528, 554, 2558, 2993
SmoI	CTYRAG	4 cut(s)	528, 554, 2558, 2993
SspMI	CTAG	2 cut(s)	1506, 3020
SstI	GAGCTC	1 cut(s)	402
StyI	CCWWGG	4 cut(s)	1604, 1676, 1929, 3019
TaaI	ACNGT	8 cut(s)	503, 662, 1766, 2091, 2194, 2531, 2552, 2732
TaiI	ACGT	9 cut(s)	776, 786, 1356, 1582, 1775, 1791, 2316, 2328, 2979
TatI	WGTACW	2 cut(s)	1560, 2397
TfiI	GAWTC	5 cut(s)	1116, 1287, 1642, 1843, 3073
TscAI	CASTG	2 cut(s)	520, 1625
TseFI	GTSAC	4 cut(s)	1907, 2470, 2720, 3038
Tsp45I	GTSAC	4 cut(s)	1907, 2470, 2720, 3038
TspDTI	ATGAA	5 cut(s)	17, 1019, 1531, 2215, 2727
TspGWI	ACGGA	4 cut(s)	624, 1452, 1842, 2641
TspMI	CCCGGG	1 cut(s)	458
TspRI	CASTG	2 cut(s)	520, 1625
Vha464I	CTTAAG	1 cut(s)	2558
VneI	GTGCAC	1 cut(s)	2546
VpaK11BI	GGWCC	7 cut(s)	231, 1811, 2087, 2181, 2296, 2956, 3016
XagI	CCTNNNNNAGG	2 cut(s)	1103, 2303
XapI	RAATTY	4 cut(s)	533, 2119, 2413, 2452
XceI	RCATGY	1 cut(s)	2404
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2413
XhoI	CTCGAG	1 cut(s)	554
XmaI	CCCGGG	1 cut(s)	458
XmaJI	CCTAGG	1 cut(s)	3019
XspI	CTAG	2 cut(s)	1506, 3020
ZraI	GACGTC	1 cut(s)	784

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.