Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0012250

Physical Location & Seq

Genomic Coordinates Forward (+)

31992960 .. 31997755

Gene Structure 4796 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1251900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3102 bp

ATGAAAGTTCCTTCCTTTTCCTCCTCCTTCCCTCTCTCTCTCATCCTCTTCTTCTCTCTCCAGACCTCCATAACTCTCTGCTCTTCTTCCTCTTCTTCTTCTCTGTCTTTCCAGCTCATTGCTCTGCTCTCCCTCAAATCCTCCCTCAAAGACCCTCTCTCCTCCTTCCACGACTGGGTTCTCCCCAATTTCCAACAAAACGGCGGCGTCACCGGTTGGTGTTCGTGGTCCGGCGTTAAATGCCACCCCAACACCTCCCAAGTCGTCGCCCTCGACCTCTCACGCCGCAACCTCTCCGGCCAAATCCCACCTCAAATCCGCCACCTTTCTTCCCTAATCTACCTCAATTTGAGCGGCAACGCCTTCGACGGCCCTTTCCCTCCCGCCATTTTCGAGCTCGGCAACTTAAGGATCCTCGACATCAGCCACAACGACTTCAAGTCGGATTTCCCGCCTGGGATTTCAAAGCTCAAGTTTTTAAGAATCTTCACGGCTTACAGCAACAGCTTCACCGGCCCATTGCCTCAGGAATTCATCAAACTCCGGTATCTCGAACGGCTCAACCTCGGCGGAAGCTACTTCGACGGCGAAATCCCGGAAGGGTACGGAAGTTTCCCGAGATTGAAATTCCTTTACCTCGCCGGAAATGCACTTAACGGTTCAATTCCACCGCAATTGGGCTTCTTATCGGAGCTGACCCGGATGGAGCTCGGGTACAACGAGTATTCAGGTGGGTTTCCTGCAGAACTCGCAATGCTGTCAAATCTCACGTACTTGGACGTCTCCAACGCCCTTCTCTCCGGCGCTCTCCCGCCGGAGCTCGGCAACTTAACCATGCTCGACACACTGCTGCTCTTCAAGAACCGATTTTCGGGATTTATTCCGGCAAGTCTAGGCCGCTTACAGGGTCTGAAGACGCTCGATTTGTCGGATAATCAGCTCTCCGGCTCAATCCCACCGGAAATGGCAACGTTGAAAAGGCTGACCAATATAAGCCTGATGAACAACTTTCTCGTCGGAGAAATCCCGGAAAAAATGGGAGAGCTGCCCAACTTGGAAAACCTCCTCCTCTGGAACAATTCACTGACGGGTGTCCTCCCCCAGAGCTTAGGATTCAGTTGGAAGCTCCTGCGAGTCGACGTTTCCTCCAATTCTCTCACCGGACCGATCCCGCCAAATCTCTGCAACGGCTACAAGCTCCAAAAGCTCATACTTTTCTCCAACAGGTTCACCGACCCACTTCCCAAAACGTTAGCAAACTGCAATTCTCTTTTACGACTCCGAATCCAGAATAACCAAATCAATGGGTCGGTTCCGACGGGTTTCGGGTTGTTACCCAATTTGACCTTCGTCGATTTGAGCGGCAACAATTTCACCGGCGCGATACCTGAAGATCTCGGCAATGCCGAAGAGCTCAGTTACTTGAACATTTCGCTGAACCCACTTCACTCAGTCCTTCCGTCGAACATCTGGCGCGCACCAAATCTCCAGATCTTCTCCGCCAGTTCATGCCACCTCACCGGTAAAATCCCCGACTTCATCGGCTGCAAAAGCCTGTACAAGATCGAACTCCAACGTAACGACTTTAACGGCTCGATCCCGTGGGACATCGGGCACTGCGAGAAGCTTCTCAGCTTGAATCTGAGCCGCAATTCTCTCACAGGCATTATCCCATGGGAAATCTCCGCTTTGCCCTCCATTACCGACCTCGACCTCTCCCGTAATTTTCTCTCCGGCACGATTCCGTCCAACTTCGAGAACTGTAGCACGCTGGAGAGTTTCAACGTTTCGTTTAATTTGTTGACCGGTCCGATCCCGTCCGGTTCGATTTTCCTGAATCTCCATCCGACGTCGTTTTTAGGGAATGAAGGATTATGTGGCGGCGTTCTGGGGAAGCCCTGTGACACGTTGTCAGCCGGAGCCGTGGACGTTCGCGGCCACGAGCAGCCAAGGAAGACGGCAGGCGCGATAGTATGGATTATGGCGGCTGCTTTCGGTATCGGGCTTTTTGTTCTCGTCGCTGGGACCCGCTGCTTCCACGCGAATTACAGCCGTCAGATGGACGAGAGTCAACAGATCGGACCGTGGAAATTAACCGCTTTCCAGCGGTTAAATTTCACGGCGGATGACGTGTTGGAGTGCCTGGAAATGAGTAATAAGATTGTAGGGATGGGGTCCACGGGGACGGTTTATAGAGCTGAGATGCCAGGTGGCGAGATCATAGCGGTGAAGAAACTGTGGGGGAAACAAAAGGAAAACAGTAGTATTGTGATCCGACGGCGGAGAGGAGTTCTGGCTGAAGTGGAGGTGTTGGGGAACGTGAGGCACAGGAACATTGTGAGATTGTTAGGATGCTGCTGCAACAGGGACATCACCATGCTGCTCTACGAGTACATGCCCAATGGCAATTTAGATGATCTGTTGCATGGCAAAAACAAGGCTCAGAATTTGGGGGCGGATTGGGTCACGAGGTACAAGATCGCCCTCGGCGTGGCGCAGGGAATTTGCTACCTGCACCACGACTGCGACCCGGTGATAGTGCACCGTGATCTTAAACCGAGTAATATTTTATTGGATAGTGAAATGGAGGCTAGGGTGGCAGATTTCGGGGTGGCCAAGTTGATCCAGAGCGATGAGTCCATGTCTGTCATCGCCGGCTCCTATGGCTACATTGCACCAGAATATGCTTATACCCTACAAGTAGATGAAAAGAGTGATATATACAGCTATGGGGTGGTGCTGATGGAGATCTTAAGCGGGAAAAGATCAGTCGACGCCGAGTTTGGAGACGGGAATAGCATAGTGCATTGGGTTAGGTCCAAGATCAAGACGAAAGACGGGATCAATGACGTTTTGGACAAGAATGCAGGGGCGGGTTGCGAGCCGGTGAGGGAGGAGATGATGCAGATGCTTAGGATTGCTTTGCTCTGCACGAGCCAGAACCCGGCGGACCGGCCGTCGATGAGGGACGTGGTGTTGATGCTGCTTGAGGCCAAGCCCAAGAGGAAGTTGCTAGCTAGTGTTGGGCATGGCCACAATGGGAGCGGTTGTGCTGAGTATCATGATATTCCTTTGCCACAAAAGCCAGCAGCGGAATGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001763 GO:0003006 GO:0005575 GO:0005623 GO:0005886 GO:0007275 GO:0008150 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009987 GO:0010016 GO:0010065 GO:0010067 GO:0010087 GO:0010089 GO:0010154 GO:0010223 GO:0010346 GO:0016020 GO:0022414 GO:0032501 GO:0032502 GO:0044464 GO:0048316 GO:0048367 GO:0048507 GO:0048508 GO:0048608 GO:0048646 GO:0048731 GO:0048856 GO:0051301 GO:0061458 GO:0071944 GO:1905393

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase

Protein Analysis

1034

Amino Acids

113.31

Weight (kDa)

6.47

Isoelectric Point (pI)

34.54

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRR_14	PF23598	251 - 478	5.5e-09	Leucine-rich repeat region
LRR_8	PF13855	399 - 458	1.4e-06	Leucine rich repeat
LRR_8	PF13855	518 - 575	5.2e-07	Leucine rich repeat
Pkinase	PF00069	719 - 992	2.8e-45	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	719 - 993	1.5e-39	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0012250)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G61480
fragaria_vesca	FvH4_7g27270
malus_domestica	MD01G1181700.v1.1 MD07G1251900.v1.1
prunus_persica	Prupe.2G275500_v2.0.a1
pyrus_communis	pycom01g19150 pycom07g22430
rosa_chinensis	RchiOBHm_Chr1g0374101
rosa_laevigata	RLG00000026763
rosa_multiflora	Rmu_co8312173.1_g000001 Rmu_co8440105.1_g000001 Rmu_sc0006924.1_g000013
rosa_roxburghii	Rroxscaffold_4G00283740
rosa_rugosa	Rorug01G0380800
rosa_samantha	Rh1AG391500 Rh1BG355500 Rh1CG368500 Rh1DG386200
rosa_wichuraiana	Rw1G034410

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	2 cut(s)	783, 1853
Acc36I	ACCTGC	1 cut(s)	2520
AccBSI	CCGCTC	3 cut(s)	354, 1362, 3045
AccI	GTMKAC	2 cut(s)	1137, 2772
AccII	CGCG	5 cut(s)	1382, 1476, 1935, 1967, 2042
AclI	AACGTT	3 cut(s)	971, 1250, 1785
AclWI	GGATC	8 cut(s)	406, 419, 1162, 1591, 1807, 2266, 2617, 2849
AcoI	YGGCCR	5 cut(s)	298, 1936, 2613, 2954, 3031
AcsI	RAATTY	5 cut(s)	530, 626, 2113, 2446, 2502
AcuI	CTGAAG	3 cut(s)	932, 1410, 2319
AcyI	GRCGYC	4 cut(s)	207, 780, 1850, 2775
AfaI	GTAC	6 cut(s)	605, 716, 773, 1559, 2393, 2474
AfiI	CCNNNNNNNGG	8 cut(s)	175, 677, 821, 871, 904, 2059, 2491, 2944
AflII	CTTAAG	2 cut(s)	406, 2752
AflIII	ACRYGT	2 cut(s)	1905, 2130
AgeI	ACCGGT	3 cut(s)	212, 1520, 1805
AgsI	TTSAA	9 cut(s)	439, 465, 625, 663, 859, 976, 1426, 1639, 1783
AhdI	GACNNNNNGTC	3 cut(s)	439, 1909, 2956
AjiI	CACGTC	2 cut(s)	2131, 2971
AjnI	CCWGG	3 cut(s)	454, 2142, 2206
AjuI	GAANNNNNNNTTGG	2 cut(s)	1331, 1363
Alw21I	GWGCWC	5 cut(s)	399, 711, 822, 1416, 2544
Alw26I	GTCTC	2 cut(s)	787, 2781
Alw44I	GTGCAC	1 cut(s)	2540
AlwI	GGATC	8 cut(s)	406, 419, 1162, 1591, 1807, 2266, 2617, 2849
AlwNI	CAGNNNCTG	1 cut(s)	910
Ama87I	CYCGRG	2 cut(s)	616, 710
AoxI	GGCC	9 cut(s)	298, 370, 514, 895, 1936, 2613, 2954, 2991, 3031
ApaLI	GTGCAC	1 cut(s)	2540
ApoI	RAATTY	5 cut(s)	530, 626, 2113, 2446, 2502
ArsI	GACNNNNNNTTYG	4 cut(s)	907, 939, 2765, 2797
AsiGI	ACCGGT	3 cut(s)	212, 1520, 1805
AspLEI	GCGC	6 cut(s)	806, 1382, 1476, 1478, 1967, 2497
AsuC2I	CCSGG	5 cut(s)	596, 700, 1028, 2531, 2945
AsuNHI	GCTAGC	1 cut(s)	3013
AvaI	CYCGRG	2 cut(s)	616, 710
AvaII	GGWCC	8 cut(s)	228, 1163, 1808, 2025, 2081, 2175, 2817, 2950
AxyI	CCTNAGG	1 cut(s)	525
BaeGI	GKGCMC	2 cut(s)	1617, 2544
BaeI	ACNNNNGTAYC	4 cut(s)	530, 563, 595, 628
BalI	TGGCCA	2 cut(s)	2615, 3033
BamHI	GGATCC	1 cut(s)	411
BanII	GRGCYC	4 cut(s)	399, 711, 822, 1416
BauI	CACGAG	3 cut(s)	1940, 2467, 2932
BbsI	GAAGAC	2 cut(s)	920, 1961
Bbv12I	GWGCWC	5 cut(s)	399, 711, 822, 1416, 2544
BccI	CCATC	5 cut(s)	697, 1851, 2053, 2164, 2737
BcgI	CGANNNNNNTGC	2 cut(s)	346, 380
BciT130I	CCWGG	3 cut(s)	456, 2144, 2208
BcnI	CCSGG	5 cut(s)	596, 700, 1028, 2531, 2945
BcoDI	GTCTC	2 cut(s)	787, 2781
BfaI	CTAG	4 cut(s)	893, 2592, 3014, 3018
BfmI	CTRYAG	2 cut(s)	741, 1762
BfoI	RGCGCY	1 cut(s)	807
BfrI	CTTAAG	2 cut(s)	406, 2752
BfuAI	ACCTGC	1 cut(s)	2520
BglII	AGATCT	3 cut(s)	1393, 1491, 2748
Bme1390I	CCNGG	8 cut(s)	456, 596, 700, 1028, 2144, 2208, 2531, 2945
Bme18I	GGWCC	8 cut(s)	228, 1163, 1808, 2025, 2081, 2175, 2817, 2950
BmeRI	GACNNNNNGTC	3 cut(s)	439, 1909, 2956
BmeT110I	CYCGRG	2 cut(s)	616, 710
BmgBI	CACGTC	2 cut(s)	2131, 2971
BmiI	GGNNCC	7 cut(s)	413, 1314, 1921, 2026, 2027, 2176, 2659
BmrFI	CCNGG	8 cut(s)	456, 596, 700, 1028, 2144, 2208, 2531, 2945
BmrI	ACTGGG	1 cut(s)	184
BmsI	GCATC	5 cut(s)	2193, 2342, 2892, 2898, 2970
BmtI	GCTAGC	1 cut(s)	3017
BmuI	ACTGGG	1 cut(s)	184
BoxI	GACNNNNGTC	3 cut(s)	1091, 1349, 2067
BpiI	GAAGAC	2 cut(s)	920, 1961
BpmI	CTGGAG	3 cut(s)	44, 1472, 1793
Bpu10I	CCTNAGC	2 cut(s)	1108, 2912
BpuEI	CTTGAG	2 cut(s)	455, 3008
BpuMI	CCSGG	5 cut(s)	596, 700, 1028, 2531, 2945
BsaAI	YACGTR	1 cut(s)	771
BsaBI	GATNNNNATC	1 cut(s)	2747
BsaHI	GRCGYC	4 cut(s)	207, 780, 1850, 2775
BsaJI	CCNNGG	9 cut(s)	455, 565, 1601, 1673, 1923, 1949, 2084, 2178, 2485
BsaWI	WCCGGW	7 cut(s)	212, 543, 958, 1160, 1520, 1805, 1820
Bsc4I	CCNNNNNNNGG	8 cut(s)	175, 677, 821, 871, 904, 2059, 2491, 2944
Bse118I	RCCGGY	8 cut(s)	212, 512, 1376, 1520, 1805, 2654, 2884, 2952
Bse1I	ACTGG	2 cut(s)	179, 1503
Bse21I	CCTNAGG	1 cut(s)	525
Bse3DI	GCAATG	5 cut(s)	117, 518, 759, 1408, 2670
Bse8I	GATNNNNATC	1 cut(s)	2747
BseBI	CCWGG	3 cut(s)	456, 2144, 2208
BseDI	CCNNGG	9 cut(s)	455, 565, 1601, 1673, 1923, 1949, 2084, 2178, 2485
BseGI	GGATG	6 cut(s)	42, 708, 1843, 2131, 2175, 2357
BseJI	GATNNNNATC	1 cut(s)	2747
BseLI	CCNNNNNNNGG	8 cut(s)	175, 677, 821, 871, 904, 2059, 2491, 2944
BseMI	GCAATG	5 cut(s)	117, 518, 759, 1408, 2670
BseMII	CTCAG	8 cut(s)	539, 1429, 1464, 1634, 1645, 2190, 2456, 3045
BseNI	ACTGG	2 cut(s)	179, 1503
BsePI	GCGCGC	1 cut(s)	1474
BseRI	GAGGAG	6 cut(s)	13, 151, 1055, 1058, 2301, 2909
BseSI	GKGCMC	2 cut(s)	1617, 2544
BseX3I	CGGCCG	1 cut(s)	2954
BseYI	CCCAGC	1 cut(s)	2021
BsgI	GTGCAG	2 cut(s)	2498, 2914
Bsh1236I	CGCG	5 cut(s)	1382, 1476, 1935, 1967, 2042
Bsh1285I	CGRYCG	1 cut(s)	2957
BshFI	GGCC	9 cut(s)	300, 372, 516, 897, 1938, 2615, 2956, 2993, 3033
BshTI	ACCGGT	3 cut(s)	212, 1520, 1805
BsiEI	CGRYCG	1 cut(s)	2957
BsiHKAI	GWGCWC	5 cut(s)	399, 711, 822, 1416, 2544
BsiHKCI	CYCGRG	2 cut(s)	616, 710
BslFI	GGGAC	5 cut(s)	1619, 2038, 2197, 2381, 2981
BslI	CCNNNNNNNGG	8 cut(s)	175, 677, 821, 871, 904, 2059, 2491, 2944
BsmAI	GTCTC	2 cut(s)	787, 2781
BsmBI	CGTCTC	2 cut(s)	787, 2781
BsmFI	GGGAC	5 cut(s)	1619, 2038, 2197, 2381, 2981
BsmI	GAATGC	1 cut(s)	2869
BsnI	GGCC	9 cut(s)	300, 372, 516, 897, 1938, 2615, 2956, 2993, 3033
BsoBI	CYCGRG	2 cut(s)	616, 710
Bsp1286I	GDGCHC	6 cut(s)	399, 711, 822, 1416, 1617, 2544
Bsp1407I	TGTACA	1 cut(s)	1557
Bsp19I	CCATGG	1 cut(s)	1673
BspANI	GGCC	9 cut(s)	300, 372, 516, 897, 1938, 2615, 2956, 2993, 3033
BspCNI	CTCAG	8 cut(s)	538, 1428, 1463, 1635, 1644, 2191, 2455, 3046
BspFNI	CGCG	5 cut(s)	1382, 1476, 1935, 1967, 2042
BspHI	TCATGA	1 cut(s)	3061
BspLI	GGNNCC	7 cut(s)	413, 1314, 1921, 2026, 2027, 2176, 2659
BspMAI	CTGCAG	1 cut(s)	745
BspMI	ACCTGC	1 cut(s)	2520
BspOI	GCTAGC	1 cut(s)	3017
BspPI	GGATC	8 cut(s)	406, 419, 1162, 1591, 1807, 2266, 2617, 2849
BspQI	GCTCTTC	3 cut(s)	88, 860, 1404
BspTI	CTTAAG	2 cut(s)	406, 2752
BsrBI	CCGCTC	3 cut(s)	354, 1362, 3045
BsrDI	GCAATG	5 cut(s)	117, 518, 759, 1408, 2670
BsrFI	RCCGGY	8 cut(s)	212, 512, 1376, 1520, 1805, 2654, 2884, 2952
BsrGI	TGTACA	1 cut(s)	1557
BsrI	ACTGG	2 cut(s)	179, 1503
BssAI	RCCGGY	8 cut(s)	212, 512, 1376, 1520, 1805, 2654, 2884, 2952
BssECI	CCNNGG	9 cut(s)	455, 565, 1601, 1673, 1923, 1949, 2084, 2178, 2485
BssHII	GCGCGC	1 cut(s)	1474
BssNI	GRCGYC	4 cut(s)	207, 780, 1850, 2775
BssSI	CACGAG	3 cut(s)	1940, 2467, 2932
BssT1I	CCWWGG	2 cut(s)	1673, 1949
Bst2BI	CACGAG	3 cut(s)	1940, 2467, 2932
Bst2UI	CCWGG	3 cut(s)	456, 2144, 2208
Bst4CI	ACNGT	7 cut(s)	659, 1763, 2085, 2188, 2238, 2261, 2546
Bst6I	CTCTTC	5 cut(s)	53, 88, 97, 860, 1404
BstACI	GRCGYC	4 cut(s)	207, 780, 1850, 2775
BstAFI	CTTAAG	2 cut(s)	406, 2752
BstAUI	TGTACA	1 cut(s)	1557
BstBAI	YACGTR	1 cut(s)	771
BstC8I	GCNNGC	7 cut(s)	1476, 1769, 1963, 2656, 2882, 3015, 3087
BstDSI	CCRYGG	5 cut(s)	1601, 1673, 1923, 2084, 2178
BstF5I	GGATG	6 cut(s)	42, 708, 1843, 2131, 2175, 2357
BstFNI	CGCG	5 cut(s)	1382, 1476, 1935, 1967, 2042
BstH2I	RGCGCY	1 cut(s)	807
BstHHI	GCGC	6 cut(s)	806, 1382, 1476, 1478, 1967, 2497
BstMAI	GTCTC	2 cut(s)	787, 2781
BstMCI	CGRYCG	1 cut(s)	2957
BstMWI	GCNNNNNNNGC	9 cut(s)	240, 513, 647, 749, 1204, 1551, 2597, 2664, 3082
BstNI	CCWGG	3 cut(s)	456, 2144, 2208
BstNSI	RCATGY	1 cut(s)	2398
BstPAI	GACNNNNGTC	3 cut(s)	1091, 1349, 2067
BstSCI	CCNGG	8 cut(s)	454, 594, 698, 1026, 2142, 2206, 2529, 2943
BstSFI	CTRYAG	2 cut(s)	741, 1762
BstSLI	GKGCMC	2 cut(s)	1617, 2544
BstUI	CGCG	5 cut(s)	1382, 1476, 1935, 1967, 2042
BstV2I	GAAGAC	2 cut(s)	920, 1961
BstX2I	RGATCY	4 cut(s)	411, 1393, 1491, 2748
BstXI	CCANNNNNNTGG	1 cut(s)	1304
BstYI	RGATCY	4 cut(s)	411, 1393, 1491, 2748
BstZI	CGGCCG	1 cut(s)	2954
Bsu36I	CCTNAGG	1 cut(s)	525
BsuRI	GGCC	9 cut(s)	300, 372, 516, 897, 1938, 2615, 2956, 2993, 3033
BtgI	CCRYGG	5 cut(s)	1601, 1673, 1923, 2084, 2178
BtgZI	GCGATG	2 cut(s)	2635, 2646
BtrI	CACGTC	2 cut(s)	2131, 2971
BtsCI	GGATG	6 cut(s)	42, 708, 1843, 2131, 2175, 2357
BtsI	GCAGTG	2 cut(s)	845, 1615
BtsIMutI	CAGTG	3 cut(s)	845, 1082, 1615
BveI	ACCTGC	1 cut(s)	2520
Cac8I	GCNNGC	7 cut(s)	1476, 1769, 1963, 2656, 2882, 3015, 3087
CaiI	CAGNNNCTG	1 cut(s)	910
CciI	TCATGA	1 cut(s)	3061
CfoI	GCGC	6 cut(s)	806, 1382, 1476, 1478, 1967, 2497
Cfr10I	RCCGGY	8 cut(s)	212, 512, 1376, 1520, 1805, 2654, 2884, 2952
CpoI	CGGWCCG	4 cut(s)	1163, 1808, 2081, 2950
CseI	GACGC	3 cut(s)	196, 925, 2783
Csp6I	GTAC	6 cut(s)	604, 715, 772, 1558, 2392, 2473
CspAI	ACCGGT	3 cut(s)	212, 1520, 1805
CspCI	CAANNNNNGTGG	2 cut(s)	657, 692
CspI	CGGWCCG	4 cut(s)	1163, 1808, 2081, 2950
CviAII	CATG	9 cut(s)	835, 1509, 1674, 2377, 2395, 2426, 2641, 3029, 3062
CviQI	GTAC	6 cut(s)	604, 715, 772, 1558, 2392, 2473
DriI	GACNNNNNGTC	3 cut(s)	439, 1909, 2956
EaeI	YGGCCR	5 cut(s)	298, 1936, 2613, 2954, 3031
EagI	CGGCCG	1 cut(s)	2954
Eam1104I	CTCTTC	5 cut(s)	53, 88, 97, 860, 1404
Eam1105I	GACNNNNNGTC	3 cut(s)	439, 1909, 2956
EarI	CTCTTC	5 cut(s)	53, 88, 97, 860, 1404
EciI	GGCGGA	7 cut(s)	308, 585, 1489, 2138, 2296, 2471, 2963
Ecl136II	GAGCTC	4 cut(s)	397, 709, 820, 1414
EclXI	CGGCCG	1 cut(s)	2954
Eco130I	CCWWGG	2 cut(s)	1673, 1949
Eco24I	GRGCYC	4 cut(s)	399, 711, 822, 1416
Eco47I	GGWCC	8 cut(s)	228, 1163, 1808, 2025, 2081, 2175, 2817, 2950
Eco52I	CGGCCG	1 cut(s)	2954
Eco53kI	GAGCTC	4 cut(s)	397, 709, 820, 1414
Eco57I	CTGAAG	3 cut(s)	932, 1410, 2319
Eco81I	CCTNAGG	1 cut(s)	525
Eco88I	CYCGRG	2 cut(s)	616, 710
EcoICRI	GAGCTC	4 cut(s)	397, 709, 820, 1414
EcoO109I	RGGNCCY	1 cut(s)	2025
EcoRI	GAATTC	1 cut(s)	530
EcoRII	CCWGG	3 cut(s)	454, 2142, 2206
EcoT14I	CCWWGG	2 cut(s)	1673, 1949
EcoT38I	GRGCYC	4 cut(s)	399, 711, 822, 1416
ErhI	CCWWGG	2 cut(s)	1673, 1949
Esp3I	CGTCTC	2 cut(s)	787, 2781
FaeI	CATG	9 cut(s)	838, 1512, 1677, 2380, 2398, 2429, 2644, 3032, 3065
FaqI	GGGAC	5 cut(s)	1619, 2038, 2197, 2381, 2981
FatI	CATG	9 cut(s)	834, 1508, 1673, 2376, 2394, 2425, 2640, 3028, 3061
FauI	CCCGC	7 cut(s)	391, 459, 819, 1179, 2036, 2750, 2866
FblI	GTMKAC	2 cut(s)	1137, 2772
FokI	GGATG	6 cut(s)	29, 715, 1830, 2138, 2182, 2364
FriOI	GRGCYC	4 cut(s)	399, 711, 822, 1416
FspBI	CTAG	4 cut(s)	893, 2592, 3014, 3018
GlaI	GCGC	6 cut(s)	805, 1381, 1475, 1477, 1966, 2496
GsaI	CCCAGC	1 cut(s)	2025
GsuI	CTGGAG	3 cut(s)	44, 1472, 1793
HaeII	RGCGCY	1 cut(s)	807
HaeIII	GGCC	9 cut(s)	300, 372, 516, 897, 1938, 2615, 2956, 2993, 3033
HgaI	GACGC	3 cut(s)	196, 925, 2783
HhaI	GCGC	6 cut(s)	806, 1382, 1476, 1478, 1967, 2497
Hin1I	GRCGYC	4 cut(s)	207, 780, 1850, 2775
Hin1II	CATG	9 cut(s)	838, 1512, 1677, 2380, 2398, 2429, 2644, 3032, 3065
Hin6I	GCGC	6 cut(s)	804, 1380, 1474, 1476, 1965, 2495
HinP1I	GCGC	6 cut(s)	804, 1380, 1474, 1476, 1965, 2495
HincII	GTYRAC	4 cut(s)	1138, 1803, 2072, 2773
HindII	GTYRAC	4 cut(s)	1138, 1803, 2072, 2773
HindIII	AAGCTT	1 cut(s)	1625
Hpy166II	GTNNAC	8 cut(s)	1138, 1230, 1803, 1927, 2072, 2178, 2542, 2773
Hpy8I	GTNNAC	8 cut(s)	1138, 1230, 1803, 1927, 2072, 2178, 2542, 2773
HpyAV	CCTTC	9 cut(s)	21, 37, 175, 373, 593, 803, 1357, 1466, 1862
HpyCH4III	ACNGT	7 cut(s)	659, 1763, 2085, 2188, 2238, 2261, 2546
HpyF10VI	GCNNNNNNNGC	9 cut(s)	240, 513, 647, 749, 1204, 1551, 2597, 2664, 3082
Hsp92I	GRCGYC	4 cut(s)	207, 780, 1850, 2775
Hsp92II	CATG	9 cut(s)	838, 1512, 1677, 2380, 2398, 2429, 2644, 3032, 3065
HspAI	GCGC	6 cut(s)	804, 1380, 1474, 1476, 1965, 2495
KflI	GGGWCCC	1 cut(s)	2025
KroI	GCCGGC	1 cut(s)	2654
KroNI	GCCGGC	1 cut(s)	2656
LguI	GCTCTTC	3 cut(s)	88, 860, 1404
LmnI	GCTCC	8 cut(s)	691, 706, 817, 1131, 1203, 1919, 2663, 3042
LweI	GCATC	5 cut(s)	2193, 2342, 2892, 2898, 2970
MaeI	CTAG	4 cut(s)	893, 2592, 3014, 3018
MaeIII	GTNAC	6 cut(s)	208, 1332, 1418, 1577, 1901, 2464
MbiI	CCGCTC	3 cut(s)	354, 1362, 3045
MfeI	CAATTG	1 cut(s)	674
MflI	RGATCY	4 cut(s)	411, 1393, 1491, 2748
MhlI	GDGCHC	6 cut(s)	399, 711, 822, 1416, 1617, 2544
MlsI	TGGCCA	2 cut(s)	2615, 3033
MluNI	TGGCCA	2 cut(s)	2615, 3033
MlyI	GAGTC	4 cut(s)	1143, 1272, 2077, 2645
Mox20I	TGGCCA	2 cut(s)	2615, 3033
MroNI	GCCGGC	1 cut(s)	2654
MscI	TGGCCA	2 cut(s)	2615, 3033
MslI	CAYNNNNRTG	2 cut(s)	2225, 2375
Msp20I	TGGCCA	2 cut(s)	2615, 3033
MspA1I	CMGCKG	3 cut(s)	2031, 2107, 3092
MspCI	CTTAAG	2 cut(s)	406, 2752
MspR9I	CCNGG	8 cut(s)	456, 596, 700, 1028, 2144, 2208, 2531, 2945
MunI	CAATTG	1 cut(s)	674
Mva1269I	GAATGC	1 cut(s)	2869
MvaI	CCWGG	3 cut(s)	456, 2144, 2208
MvnI	CGCG	5 cut(s)	1382, 1476, 1935, 1967, 2042
MwoI	GCNNNNNNNGC	9 cut(s)	240, 513, 647, 749, 1204, 1551, 2597, 2664, 3082
NaeI	GCCGGC	1 cut(s)	2656
NciI	CCSGG	5 cut(s)	596, 700, 1028, 2531, 2945
NcoI	CCATGG	1 cut(s)	1673
NgoMIV	GCCGGC	1 cut(s)	2654
NheI	GCTAGC	1 cut(s)	3013
NlaIII	CATG	9 cut(s)	838, 1512, 1677, 2380, 2398, 2429, 2644, 3032, 3065
NlaIV	GGNNCC	7 cut(s)	413, 1314, 1921, 2026, 2027, 2176, 2659
NmeAIII	GCCGAG	6 cut(s)	378, 546, 801, 1377, 2466, 2803
NmuCI	GTSAC	3 cut(s)	208, 1901, 2464
NspI	RCATGY	1 cut(s)	2398
PagI	TCATGA	1 cut(s)	3061
PauI	GCGCGC	1 cut(s)	1474
PciSI	GCTCTTC	3 cut(s)	88, 860, 1404
PcsI	WCGNNNNNNNCGW	6 cut(s)	270, 717, 1404, 1617, 1964, 2487
PctI	GAATGC	1 cut(s)	2869
PdiI	GCCGGC	1 cut(s)	2656
PfeI	GAWTC	6 cut(s)	483, 1113, 1284, 1639, 1741, 1837
PfoI	TCCNGGA	2 cut(s)	594, 1026
PinAI	ACCGGT	3 cut(s)	212, 1520, 1805
PleI	GAGTC	4 cut(s)	1142, 1272, 2076, 2644
PpsI	GAGTC	4 cut(s)	1142, 1272, 2076, 2644
Ppu21I	YACGTR	1 cut(s)	771
PpuMI	RGGWCCY	1 cut(s)	2025
PshAI	GACNNNNGTC	3 cut(s)	1091, 1349, 2067
Psp124BI	GAGCTC	4 cut(s)	399, 711, 822, 1416
Psp1406I	AACGTT	3 cut(s)	971, 1250, 1785
Psp5II	RGGWCCY	1 cut(s)	2025
Psp6I	CCWGG	3 cut(s)	454, 2142, 2206
PspFI	CCCAGC	1 cut(s)	2021
PspGI	CCWGG	3 cut(s)	454, 2142, 2206
PspN4I	GGNNCC	7 cut(s)	413, 1314, 1921, 2026, 2027, 2176, 2659
PspPPI	RGGWCCY	1 cut(s)	2025
PstI	CTGCAG	1 cut(s)	745
PstNI	CAGNNNCTG	1 cut(s)	910
PsuI	RGATCY	4 cut(s)	411, 1393, 1491, 2748
PteI	GCGCGC	1 cut(s)	1474
RsaI	GTAC	6 cut(s)	605, 716, 773, 1559, 2393, 2474
RsaNI	GTAC	6 cut(s)	604, 715, 772, 1558, 2392, 2473
RseI	CAYNNNNRTG	2 cut(s)	2225, 2375
Rsr2I	CGGWCCG	4 cut(s)	1163, 1808, 2081, 2950
RsrII	CGGWCCG	4 cut(s)	1163, 1808, 2081, 2950
SacI	GAGCTC	4 cut(s)	399, 711, 822, 1416
SalI	GTCGAC	2 cut(s)	1136, 2771
SapI	GCTCTTC	3 cut(s)	88, 860, 1404
SchI	GAGTC	4 cut(s)	1143, 1272, 2077, 2645
ScrFI	CCNGG	8 cut(s)	456, 596, 700, 1028, 2144, 2208, 2531, 2945
SduI	GDGCHC	6 cut(s)	399, 711, 822, 1416, 1617, 2544
SfaNI	GCATC	5 cut(s)	2193, 2342, 2892, 2898, 2970
SfcI	CTRYAG	2 cut(s)	741, 1762
SgrAI	CRCCGGYG	1 cut(s)	1376
SinI	GGWCC	8 cut(s)	228, 1163, 1808, 2025, 2081, 2175, 2817, 2950
SmiMI	CAYNNNNRTG	2 cut(s)	2225, 2375
SmlI	CTYRAG	4 cut(s)	406, 470, 2752, 2987
SmoI	CTYRAG	4 cut(s)	406, 470, 2752, 2987
SspI	AATATT	1 cut(s)	2566
SspMI	CTAG	4 cut(s)	893, 2592, 3014, 3018
SstI	GAGCTC	4 cut(s)	399, 711, 822, 1416
StyD4I	CCNGG	8 cut(s)	454, 594, 698, 1026, 2142, 2206, 2529, 2943
StyI	CCWWGG	2 cut(s)	1673, 1949
TaaI	ACNGT	7 cut(s)	659, 1763, 2085, 2188, 2238, 2261, 2546
TaqII	GACCGA	1 cut(s)	1180
TatI	WGTACW	2 cut(s)	1557, 2391
TauI	GCSGC	9 cut(s)	207, 288, 357, 900, 1365, 1650, 1884, 1938, 1988
TfiI	GAWTC	6 cut(s)	483, 1113, 1284, 1639, 1741, 1837
TscAI	CASTG	3 cut(s)	852, 1089, 1622
TseFI	GTSAC	3 cut(s)	208, 1901, 2464
Tsp45I	GTSAC	3 cut(s)	208, 1901, 2464
TspDTI	ATGAA	7 cut(s)	17, 523, 1016, 1497, 1528, 1881, 2721
TspGWI	ACGGA	3 cut(s)	621, 1449, 1734
TspRI	CASTG	3 cut(s)	852, 1089, 1622
Vha464I	CTTAAG	2 cut(s)	406, 2752
VneI	GTGCAC	1 cut(s)	2540
VpaK11BI	GGWCC	8 cut(s)	228, 1163, 1808, 2025, 2081, 2175, 2817, 2950
XapI	RAATTY	5 cut(s)	530, 626, 2113, 2446, 2502
XceI	RCATGY	1 cut(s)	2398
XmiI	GTMKAC	2 cut(s)	1137, 2772
XspI	CTAG	4 cut(s)	893, 2592, 3014, 3018
ZraI	GACGTC	2 cut(s)	781, 1851

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.