Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr1

Orthogroup ID

OG0012250

Physical Location & Seq

Genomic Coordinates Reverse (-)

62318389 .. 62322581

Gene Structure 4193 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw1G034410.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3069 bp

ATGAAACCTCCCTTTCTTCGCTTTCTCTTCTTCTTCTCTCTCTTCTTTCTTCAAGCATCCATAGTCCTCTGTGCTTCAAATTCTGCACCTTCCCAACTCAGTGCCTTGCTCTCCCTCAAATCCTCACTCAAGGATCCTCTCTTTACATTCAATGGCTGGGAGAGCAAGGCTAATACTGAAGGTCCAGTTTGGTGTTCATGGTCTGGAATCAAATGCCACCCCAACACATCCCTAGTAGTCTCTCTAGACCTCTCTCACCGCAATCTTTCCGGTACAATACCAGCTGAAATTCGCTACTTGTCGAGCTTGATTCACCTAAACTTGAGCGGAAACAGCTTTGCTGGACTTCTGCAACCAGCCATTTTCCAACTCACCCAGCTCAGAATCCTTGACATAAGCCACAACGACTTCAACTTAACTTTCCCGCCTGGGATTTCCAAGCTCAAGTTCTTGAGGGTCTTCAATGCTTACAGCAACAGCTTCACTGGTCCTTTACCTCAAGAAATCATCCGACTCAGGTTCCTCGAGAAGCTCAACCTTAGTGGAAGCTACTTCGAAGGTAATATTCCACCAGGGTATGGAAATATTCCCCAACTCCGGGAGCTTGGCCTCGCTGGAAATGTTCTTAAAGGCGCAATACCACCTCAACTGGGGTTGTTATCAGAGCTCACTCGCATGGAGATCGGCTACAACGGCTTCACAGGTGGGCTTCCGGTACAATTGACATTACTATCAAACCTCACCTACTTAGACATCTCCAATAACTTGCTTTCCGGGTCACTCCCACCTGAGCTTGGCAATTTAACCATGCTCAACACATTGTTCCTCTTCAAAAACCAATTTTCGGGCACTATCCCACCCACCCTCGGAAACTTACAAGCTCTCCAATTCCTCGACTTATCAGATAATCAGCTTTCGGGTTCCATCCCAGCCGCCATTGCTACGCTGAAACAGCTCAACAGGCTGAGCTTAATGAACAATTTTTTAGTTGGCGAAATCCCATCCCAAATCGGAGAGCTTCCCTACTTGGATACCCTCCTTCTCTGGAACAACTCCCTCACAGGGTCCCTCCCCCAAAACCTCGGCTCCAATGCGAAGCTCGTCCTCGTCGACCTCTCTTCCAACTCCCTCACAGGTCCAATTCCCCCAACTCTCTGCAAAGGCAACACACTCGTCAAGCTCATCCTCTTCTCAAACAGATTCACCAGCCATCTTCCACCAAATTTAGCCAGTTGTACATCCTTGTTCCGATTCCGCATCCAAAACAACCAACTTAACGGCTCAATCCCTCAGGGCTTCGGCTCCCTCCCCAACCTAACCTTCTTCGACCTCAGCAACAACAGCTTCTCCGGGCCCATCCCCTCCGATCTCGGCAATGCCGCGAAGCTCCAGTACCTAAACATCTCCCAGAACCCTCTCCACACACTCCTCCCATCAAACATCTGGGGCGCTGTTAATCTGCAGATATTCTCCGCCATCTCCAGCCACCTCACCGGAAAAATCCCCGACTTCATCGGCTGCAAGAGCGTCTACAAGATCGAATTACAACGCAACAACTTCACCGGCAGCATTCCCTGGGACATTGGCCACTGCGAGAAGCTTCTGAGCTTAAGCCTGAGCCGGAACTCACTCACCGGAATTATACCATGGGAAATTTCAGCTCTGCCTTCCATAACCGACCTGGACCTCTCCTACAACTTCCTCTCCGGTACTATCCCCTCGAATTTCGAGAATTGCAGAACGCTGGAGACCTTTAACGTCTCGTTTAATCTGTTAACCGGTCCTATTCCGTCTTCCGGTGCGATCTTCCCGAACCTTCATCCCACGTCGTTTTCCGGGAATGAGGGGCTATGCGGCGCCGTTTTGGCCAAGCCGTGCGCGGCGGATACTCTAACGGCTGGAGCTGTTGAGGTTGATGGTCACGAGCAGCCGAAGAAGACGGCCGGCGCGATTGTGTGGATCATGGCGGCGGCGTTCGGGATCGGTCTCTTCGTCCTCGTCGCCGGGACGAGGTGCTTCCACGCGAGCTACAGCCGTCGGATGGAGGATAGCCCCCAGATCGGACCGTGGAAATTGACCGCCTTCCAGCGTTTGAATTTCACGGCGGAGGATGTGCTGGAGTGTTTGCAGATGAGCGATAAGGTGATAGGGATGGGTGGGACCGGGACGGTGTATAAGGCGGAGATGCCAGGTGGGGAGATCATAGCGGTGAAGAAGCTGTGGGGGAAGCAGAAGGAGAGCATAATCCGACGGCGGAGAGGGGTGCTGGCCGAGGTGGAGGTGTTGGGGAACGTGAGGCATAGGAATATAGTGAGATTGTTAGGGTGCTGCTGCAACAGGGACTGCACCATGCTGCTCTACGAGTACATGCCCAATGGCAACTTGGACGACTTGTTGCATGGCAAGAACAAGGCCCAGAATTTGGGCGCGGATTGGGTCACGAGGTACAAGATTGCCCTCGGTGTCGCCCAAGGGATTTGCTACTTACATCACGACTGTGATCCGGTGATCGTCCACCGCGATCTCAAGCCGAGTAACATCCTCTTGGACGGTGACATGGAGGCGAGAGTGGCGGACTTTGGGGTGGCTAAGCTGATCCAGAGCGATGAGTCCATGTCGGTGATCGCCGGTTCCTACGGCTACATTGCACCAGAGTATGCTTATACCCTCCAAGTTGATGAGAAGAGTGACATATACAGCTTCGGGGTTGTGCTATTGGAGATTTTAAGCGGAAAGAGATCGGTGGAGGCGATTTTCGGGGATGGGAATAGCATTGTGGATTGGGTTCGGACGAAAATCAAGAGTAAAGACGGGATCAACGATGTTTTGGACAAGAATGCCGGGGCAGGTTGTACGCCGGTGAGGGAGGAGATGATGCAAATGCTTCGGATTGCGTTGCTTTGCACGAGTAGGAATCCGGCGGACCGGCCGTCTATGAGGGATGTGGTGTTGATGCTCATTGAAGCCAAACCTAAGAGGAAGTTGCTTGCTAGTCATGTTGTTGGTCGTGATCGAAGTGTGAGTTGTGGTGATATTCCTTTGCCACAAAAGCCAACAATGGAATGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0001763 GO:0003006 GO:0005575 GO:0005623 GO:0005886 GO:0007275 GO:0008150 GO:0009653 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0009987 GO:0010016 GO:0010065 GO:0010067 GO:0010087 GO:0010089 GO:0010154 GO:0010223 GO:0010346 GO:0016020 GO:0022414 GO:0032501 GO:0032502 GO:0044464 GO:0048316 GO:0048367 GO:0048507 GO:0048508 GO:0048608 GO:0048646 GO:0048731 GO:0048856 GO:0051301 GO:0061458 GO:0071944 GO:1905393

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase

Protein Analysis

1022

Amino Acids

111.64

Weight (kDa)

8.09

Isoelectric Point (pI)

33.86

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRRNT_2	PF08263	30 - 72	5e-06	Leucine rich repeat N-terminal domain
LRR_8	PF13855	80 - 137	5.2e-06	Leucine rich repeat
LRR_14	PF23598	284 - 361	6.9e-07	Leucine-rich repeat region
LRR_8	PF13855	509 - 566	1.9e-07	Leucine rich repeat
Pkinase	PF00069	713 - 982	3.9e-45	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	713 - 984	1.8e-39	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012250)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G61480
fragaria_vesca	FvH4_7g27270
malus_domestica	MD01G1181700.v1.1 MD07G1251900.v1.1
prunus_persica	Prupe.2G275500_v2.0.a1
pyrus_communis	pycom01g19150 pycom07g22430
rosa_chinensis	RchiOBHm_Chr1g0374101
rosa_laevigata	RLG00000026763
rosa_multiflora	Rmu_co8312173.1_g000001 Rmu_co8440105.1_g000001 Rmu_sc0006924.1_g000013
rosa_roxburghii	Rroxscaffold_4G00283740
rosa_rugosa	Rorug01G0380800
rosa_samantha	Rh1AG391500 Rh1BG355500 Rh1CG368500 Rh1DG386200
rosa_wichuraiana	Rw1G034410

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	2837
AccB1I	GGYRCC	1 cut(s)	1856
AccB7I	CCANNNNNTGG	2 cut(s)	578, 2422
AccBSI	CCGCTC	1 cut(s)	327
AccI	GTMKAC	2 cut(s)	1110, 1530
AccII	CGCG	6 cut(s)	1382, 1880, 1949, 2024, 2429, 2520
AclWI	GGATC	7 cut(s)	128, 141, 1967, 1988, 2495, 2590, 2822
AcoI	YGGCCR	5 cut(s)	1585, 1866, 1941, 2268, 2927
AcsI	RAATTY	7 cut(s)	79, 288, 1222, 1653, 1723, 2095, 2419
AcuI	CTGAAG	1 cut(s)	198
AcyI	GRCGYC	1 cut(s)	1857
AfaI	GTAC	8 cut(s)	274, 717, 1237, 1394, 1711, 2366, 2447, 2854
AflII	CTTAAG	1 cut(s)	1609
AgeI	ACCGGT	1 cut(s)	1778
AgsI	TTSAA	8 cut(s)	53, 78, 151, 412, 463, 832, 2095, 2963
AhdI	GACNNNNNGTC	1 cut(s)	2929
AjiI	CACGTC	1 cut(s)	1827
AjnI	CCWGG	5 cut(s)	427, 571, 1574, 1680, 2188
AjuI	GAANNNNNNNTTGG	4 cut(s)	431, 463, 1304, 1336
AleI	CACNNNNGTG	1 cut(s)	2496
Alw21I	GWGCWC	1 cut(s)	669
Alw26I	GTCTC	4 cut(s)	244, 1742, 1765, 1991
AlwI	GGATC	7 cut(s)	128, 141, 1967, 1988, 2495, 2590, 2822
AlwNI	CAGNNNCTG	1 cut(s)	2343
Ama87I	CYCGRG	1 cut(s)	524
AoxI	GGCC	8 cut(s)	607, 1352, 1585, 1866, 1941, 2268, 2412, 2927
ApaI	GGGCCC	1 cut(s)	1356
ApeKI	GCWGC	6 cut(s)	1518, 1566, 1927, 2328, 2331, 2353
ApoI	RAATTY	7 cut(s)	79, 288, 1222, 1653, 1723, 2095, 2419
AsiGI	ACCGGT	1 cut(s)	1778
AspLEI	GCGC	6 cut(s)	635, 1451, 1859, 1880, 1949, 2429
AsuC2I	CCSGG	7 cut(s)	599, 775, 1351, 1837, 2005, 2164, 2842
AsuII	TTCGAA	1 cut(s)	555
AvaI	CYCGRG	1 cut(s)	524
AvaII	GGWCC	9 cut(s)	182, 488, 1065, 1136, 1684, 1781, 2063, 2160, 2923
AxyI	CCTNAGG	1 cut(s)	1290
BaeGI	GKGCMC	2 cut(s)	851, 1356
BalI	TGGCCA	2 cut(s)	1587, 1868
BamHI	GGATCC	1 cut(s)	133
BanI	GGYRCC	1 cut(s)	1856
BanII	GRGCYC	2 cut(s)	669, 1356
BarI	GAAGNNNNNNTAC	2 cut(s)	1376, 1408
BauI	CACGAG	3 cut(s)	1922, 2440, 2905
BbsI	GAAGAC	3 cut(s)	451, 1785, 1943
Bbv12I	GWGCWC	1 cut(s)	669
BbvCI	CCTCAGC	1 cut(s)	1331
BbvI	GCAGC	6 cut(s)	1505, 1578, 1939, 2315, 2318, 2340
BcgI	CGANNNNNNTGC	6 cut(s)	664, 698, 1153, 1187, 2867, 2901
BciT130I	CCWGG	5 cut(s)	429, 573, 1576, 1682, 2190
BciVI	GTATCC	2 cut(s)	1024, 1879
BcnI	CCSGG	7 cut(s)	599, 775, 1351, 1837, 2005, 2164, 2842
BcoDI	GTCTC	4 cut(s)	244, 1742, 1765, 1991
BfaI	CTAG	3 cut(s)	233, 245, 2991
BfmI	CTRYAG	2 cut(s)	1460, 2029
BfoI	RGCGCY	2 cut(s)	1452, 1860
BfrI	CTTAAG	1 cut(s)	1609
BfuAI	ACCTGC	1 cut(s)	2837
BfuI	GTATCC	2 cut(s)	1024, 1879
BglI	GCCNNNNNGGC	1 cut(s)	1865
BlpI	GCTNAGC	2 cut(s)	965, 2589
Bme18I	GGWCC	9 cut(s)	182, 488, 1065, 1136, 1684, 1781, 2063, 2160, 2923
BmeRI	GACNNNNNGTC	1 cut(s)	2929
BmeT110I	CYCGRG	1 cut(s)	524
BmgBI	CACGTC	1 cut(s)	1827
BmrI	ACTGGG	1 cut(s)	659
BmsI	GCATC	5 cut(s)	65, 1266, 2175, 2865, 2943
BmuI	ACTGGG	1 cut(s)	659
BpiI	GAAGAC	3 cut(s)	451, 1785, 1943
BpmI	CTGGAG	5 cut(s)	1373, 1465, 1766, 1920, 2138
Bpu10I	CCTNAGC	3 cut(s)	789, 1331, 1616
Bpu1102I	GCTNAGC	2 cut(s)	965, 2589
Bpu14I	TTCGAA	1 cut(s)	555
BpuEI	CTTGAG	6 cut(s)	113, 343, 428, 472, 483, 2510
BpuMI	CCSGG	7 cut(s)	599, 775, 1351, 1837, 2005, 2164, 2842
BsaHI	GRCGYC	1 cut(s)	1857
BsaI	GGTCTC	2 cut(s)	1742, 1991
BsaWI	WCCGGW	8 cut(s)	269, 712, 1493, 1634, 1706, 1778, 1796, 2503
BsaXI	ACNNNNNCTCC	8 cut(s)	867, 897, 1070, 1100, 1331, 1361, 1688, 1718
Bse118I	RCCGGY	6 cut(s)	1562, 1778, 1943, 2627, 2857, 2925
Bse1I	ACTGG	5 cut(s)	185, 490, 654, 1230, 1390
Bse21I	CCTNAGG	1 cut(s)	1290
Bse3DI	GCAATG	3 cut(s)	936, 1381, 2643
BseBI	CCWGG	5 cut(s)	429, 573, 1576, 1682, 2190
BseMI	GCAATG	3 cut(s)	936, 1381, 2643
BseMII	CTCAG	9 cut(s)	112, 394, 529, 780, 956, 1304, 1345, 1595, 1607
BseNI	ACTGG	5 cut(s)	185, 490, 654, 1230, 1390
BseRI	GAGGAG	2 cut(s)	1418, 2882
BseSI	GKGCMC	2 cut(s)	851, 1356
BseX3I	CGGCCG	2 cut(s)	1941, 2927
BseXI	GCAGC	6 cut(s)	1505, 1578, 1939, 2315, 2318, 2340
BseYI	CCCAGC	3 cut(s)	156, 375, 928
BsgI	GTGCAG	2 cut(s)	69, 2329
Bsh1236I	CGCG	6 cut(s)	1382, 1880, 1949, 2024, 2429, 2520
Bsh1285I	CGRYCG	2 cut(s)	1944, 2930
BshFI	GGCC	8 cut(s)	609, 1354, 1587, 1868, 1943, 2270, 2414, 2929
BshNI	GGYRCC	1 cut(s)	1856
BshTI	ACCGGT	1 cut(s)	1778
BsiEI	CGRYCG	2 cut(s)	1944, 2930
BsiHKAI	GWGCWC	1 cut(s)	669
BsiHKCI	CYCGRG	1 cut(s)	524
BslFI	GGGAC	6 cut(s)	1051, 1592, 2020, 2173, 2179, 2354
BsmAI	GTCTC	4 cut(s)	244, 1742, 1765, 1991
BsmBI	CGTCTC	1 cut(s)	1765
BsmFI	GGGAC	6 cut(s)	1051, 1592, 2020, 2173, 2179, 2354
BsmI	GAATGC	2 cut(s)	1569, 2842
BsnI	GGCC	8 cut(s)	609, 1354, 1587, 1868, 1943, 2270, 2414, 2929
Bso31I	GGTCTC	2 cut(s)	1742, 1991
BsoBI	CYCGRG	1 cut(s)	524
Bsp119I	TTCGAA	1 cut(s)	555
Bsp120I	GGGCCC	1 cut(s)	1352
Bsp1286I	GDGCHC	3 cut(s)	669, 851, 1356
Bsp1407I	TGTACA	1 cut(s)	1235
Bsp1720I	GCTNAGC	2 cut(s)	965, 2589
Bsp19I	CCATGG	1 cut(s)	1646
BspANI	GGCC	8 cut(s)	609, 1354, 1587, 1868, 1943, 2270, 2414, 2929
BspCNI	CTCAG	9 cut(s)	111, 393, 528, 781, 957, 1303, 1344, 1596, 1608
BspFNI	CGCG	6 cut(s)	1382, 1880, 1949, 2024, 2429, 2520
BspMAI	CTGCAG	1 cut(s)	1464
BspMI	ACCTGC	1 cut(s)	2837
BspPI	GGATC	7 cut(s)	128, 141, 1967, 1988, 2495, 2590, 2822
BspT104I	TTCGAA	1 cut(s)	555
BspT107I	GGYRCC	1 cut(s)	1856
BspTI	CTTAAG	1 cut(s)	1609
BspTNI	GGTCTC	2 cut(s)	1742, 1991
BsrBI	CCGCTC	1 cut(s)	327
BsrDI	GCAATG	3 cut(s)	936, 1381, 2643
BsrFI	RCCGGY	6 cut(s)	1562, 1778, 1943, 2627, 2857, 2925
BsrGI	TGTACA	1 cut(s)	1235
BsrI	ACTGG	5 cut(s)	185, 490, 654, 1230, 1390
BssAI	RCCGGY	6 cut(s)	1562, 1778, 1943, 2627, 2857, 2925
BssNI	GRCGYC	1 cut(s)	1857
BssSI	CACGAG	3 cut(s)	1922, 2440, 2905
BssT1I	CCWWGG	2 cut(s)	1646, 2470
Bst2BI	CACGAG	3 cut(s)	1922, 2440, 2905
Bst2UI	CCWGG	5 cut(s)	429, 573, 1576, 1682, 2190
Bst4CI	ACNGT	4 cut(s)	2067, 2170, 2498, 2552
Bst6I	CTCTTC	7 cut(s)	32, 47, 833, 1123, 1193, 1994, 2678
BstACI	GRCGYC	1 cut(s)	1857
BstAFI	CTTAAG	1 cut(s)	1609
BstAUI	TGTACA	1 cut(s)	1235
BstBI	TTCGAA	1 cut(s)	555
BstC8I	GCNNGC	4 cut(s)	1945, 2026, 2268, 2988
BstDSI	CCRYGG	2 cut(s)	1646, 2066
BstFNI	CGCG	6 cut(s)	1382, 1880, 1949, 2024, 2429, 2520
BstH2I	RGCGCY	2 cut(s)	1452, 1860
BstHHI	GCGC	6 cut(s)	635, 1451, 1859, 1880, 1949, 2429
BstMAI	GTCTC	4 cut(s)	244, 1742, 1765, 1991
BstMCI	CGRYCG	2 cut(s)	1944, 2930
BstNI	CCWGG	5 cut(s)	429, 573, 1576, 1682, 2190
BstNSI	RCATGY	1 cut(s)	2371
BstSFI	CTRYAG	2 cut(s)	1460, 2029
BstSLI	GKGCMC	2 cut(s)	851, 1356
BstUI	CGCG	6 cut(s)	1382, 1880, 1949, 2024, 2429, 2520
BstV1I	GCAGC	6 cut(s)	1505, 1578, 1939, 2315, 2318, 2340
BstV2I	GAAGAC	3 cut(s)	451, 1785, 1943
BstX2I	RGATCY	1 cut(s)	133
BstYI	RGATCY	1 cut(s)	133
BstZI	CGGCCG	2 cut(s)	1941, 2927
Bsu36I	CCTNAGG	1 cut(s)	1290
BsuI	GTATCC	2 cut(s)	1024, 1879
BsuRI	GGCC	8 cut(s)	609, 1354, 1587, 1868, 1943, 2270, 2414, 2929
BtgI	CCRYGG	2 cut(s)	1646, 2066
BtgZI	GCGATG	1 cut(s)	2619
BtrI	CACGTC	1 cut(s)	1827
BtsI	GCAGTG	1 cut(s)	1588
BtsIMutI	CAGTG	3 cut(s)	106, 483, 1588
BveI	ACCTGC	1 cut(s)	2837
Cac8I	GCNNGC	4 cut(s)	1945, 2026, 2268, 2988
CaiI	CAGNNNCTG	1 cut(s)	2343
CfoI	GCGC	6 cut(s)	635, 1451, 1859, 1880, 1949, 2429
Cfr10I	RCCGGY	6 cut(s)	1562, 1778, 1943, 2627, 2857, 2925
CpoI	CGGWCCG	2 cut(s)	2063, 2923
CseI	GACGC	1 cut(s)	1516
Csp6I	GTAC	8 cut(s)	273, 716, 1236, 1393, 1710, 2365, 2446, 2853
CspAI	ACCGGT	1 cut(s)	1778
CspCI	CAANNNNNGTGG	2 cut(s)	523, 558
CspI	CGGWCCG	2 cut(s)	2063, 2923
CviQI	GTAC	8 cut(s)	273, 716, 1236, 1393, 1710, 2365, 2446, 2853
DinI	GGCGCC	1 cut(s)	1858
DriI	GACNNNNNGTC	1 cut(s)	2929
EaeI	YGGCCR	5 cut(s)	1585, 1866, 1941, 2268, 2927
EagI	CGGCCG	2 cut(s)	1941, 2927
Eam1104I	CTCTTC	7 cut(s)	32, 47, 833, 1123, 1193, 1994, 2678
Eam1105I	GACNNNNNGTC	1 cut(s)	2929
EarI	CTCTTC	7 cut(s)	32, 47, 833, 1123, 1193, 1994, 2678
EciI	GGCGGA	7 cut(s)	1462, 1898, 2120, 2195, 2269, 2588, 2936
Ecl136II	GAGCTC	1 cut(s)	667
EclXI	CGGCCG	2 cut(s)	1941, 2927
Eco130I	CCWWGG	2 cut(s)	1646, 2470
Eco24I	GRGCYC	2 cut(s)	669, 1356
Eco31I	GGTCTC	2 cut(s)	1742, 1991
Eco47I	GGWCC	9 cut(s)	182, 488, 1065, 1136, 1684, 1781, 2063, 2160, 2923
Eco52I	CGGCCG	2 cut(s)	1941, 2927
Eco53kI	GAGCTC	1 cut(s)	667
Eco57I	CTGAAG	1 cut(s)	198
Eco81I	CCTNAGG	1 cut(s)	1290
Eco88I	CYCGRG	1 cut(s)	524
EcoICRI	GAGCTC	1 cut(s)	667
EcoO109I	RGGNCCY	1 cut(s)	1065
EcoRII	CCWGG	5 cut(s)	427, 571, 1574, 1680, 2188
EcoT14I	CCWWGG	2 cut(s)	1646, 2470
EcoT38I	GRGCYC	2 cut(s)	669, 1356
EgeI	GGCGCC	1 cut(s)	1858
EheI	GGCGCC	1 cut(s)	1858
ErhI	CCWWGG	2 cut(s)	1646, 2470
Esp3I	CGTCTC	1 cut(s)	1765
FaqI	GGGAC	6 cut(s)	1051, 1592, 2020, 2173, 2179, 2354
FauI	CCCGC	1 cut(s)	432
FblI	GTMKAC	2 cut(s)	1110, 1530
FriOI	GRGCYC	2 cut(s)	669, 1356
FspBI	CTAG	3 cut(s)	233, 245, 2991
GlaI	GCGC	6 cut(s)	634, 1450, 1858, 1879, 1948, 2428
GsaI	CCCAGC	3 cut(s)	160, 379, 932
GsuI	CTGGAG	5 cut(s)	1373, 1465, 1766, 1920, 2138
HaeII	RGCGCY	2 cut(s)	1452, 1860
HaeIII	GGCC	8 cut(s)	609, 1354, 1587, 1868, 1943, 2270, 2414, 2929
HgaI	GACGC	1 cut(s)	1516
HhaI	GCGC	6 cut(s)	635, 1451, 1859, 1880, 1949, 2429
Hin1I	GRCGYC	1 cut(s)	1857
Hin6I	GCGC	6 cut(s)	633, 1449, 1857, 1878, 1947, 2427
HinP1I	GCGC	6 cut(s)	633, 1449, 1857, 1878, 1947, 2427
HincII	GTYRAC	2 cut(s)	1111, 1776
HindII	GTYRAC	2 cut(s)	1111, 1776
HindIII	AAGCTT	1 cut(s)	1598
HinfI	GANTC	8 cut(s)	207, 310, 384, 513, 1200, 1251, 2609, 2914
HpaI	GTTAAC	1 cut(s)	1776
Hpy166II	GTNNAC	4 cut(s)	1111, 1531, 1776, 2515
Hpy8I	GTNNAC	4 cut(s)	1111, 1531, 1776, 2515
Hpy99I	CGWCG	5 cut(s)	1112, 1831, 2003, 2040, 2253
HpyAV	CCTTC	9 cut(s)	99, 173, 551, 1049, 1330, 1677, 1826, 2092, 2227
HpyCH4III	ACNGT	4 cut(s)	2067, 2170, 2498, 2552
HpyCH4IV	ACGT	3 cut(s)	1758, 1826, 2292
HpySE526I	ACGT	3 cut(s)	1758, 1826, 2292
Hsp92I	GRCGYC	1 cut(s)	1857
HspAI	GCGC	6 cut(s)	633, 1449, 1857, 1878, 1947, 2427
KasI	GGCGCC	1 cut(s)	1856
KflI	GGGWCCC	1 cut(s)	1065
KroI	GCCGGC	1 cut(s)	1943
KroNI	GCCGGC	1 cut(s)	1945
KspAI	GTTAAC	1 cut(s)	1776
LmnI	GCTCC	5 cut(s)	601, 1091, 1307, 1392, 1901
Lsp1109I	GCAGC	6 cut(s)	1505, 1578, 1939, 2315, 2318, 2340
LweI	GCATC	5 cut(s)	65, 1266, 2175, 2865, 2943
MaeI	CTAG	3 cut(s)	233, 245, 2991
MaeII	ACGT	3 cut(s)	1758, 1826, 2292
MaeIII	GTNAC	6 cut(s)	777, 1919, 2437, 2534, 2552, 2687
MbiI	CCGCTC	1 cut(s)	327
MfeI	CAATTG	1 cut(s)	719
MflI	RGATCY	1 cut(s)	133
MhlI	GDGCHC	3 cut(s)	669, 851, 1356
MlsI	TGGCCA	2 cut(s)	1587, 1868
MluNI	TGGCCA	2 cut(s)	1587, 1868
Mly113I	GGCGCC	1 cut(s)	1857
MlyI	GAGTC	2 cut(s)	507, 2618
MmeI	TCCRAC	5 cut(s)	391, 535, 1146, 2018, 2272
Mox20I	TGGCCA	2 cut(s)	1587, 1868
MroNI	GCCGGC	1 cut(s)	1943
MscI	TGGCCA	2 cut(s)	1587, 1868
MslI	CAYNNNNRTG	4 cut(s)	674, 2207, 2496, 2618
Msp20I	TGGCCA	2 cut(s)	1587, 1868
MspA1I	CMGCKG	1 cut(s)	284
MspCI	CTTAAG	1 cut(s)	1609
MunI	CAATTG	1 cut(s)	719
Mva1269I	GAATGC	2 cut(s)	1569, 2842
MvaI	CCWGG	5 cut(s)	429, 573, 1576, 1682, 2190
MvnI	CGCG	6 cut(s)	1382, 1880, 1949, 2024, 2429, 2520
NaeI	GCCGGC	1 cut(s)	1945
NarI	GGCGCC	1 cut(s)	1857
NciI	CCSGG	7 cut(s)	599, 775, 1351, 1837, 2005, 2164, 2842
NcoI	CCATGG	1 cut(s)	1646
NgoMIV	GCCGGC	1 cut(s)	1943
NmeAIII	GCCGAG	4 cut(s)	1062, 1350, 2296, 2556
NmuCI	GTSAC	5 cut(s)	777, 1919, 2437, 2552, 2687
NspI	RCATGY	1 cut(s)	2371
NspV	TTCGAA	1 cut(s)	555
OliI	CACNNNNGTG	1 cut(s)	2496
PaeR7I	CTCGAG	1 cut(s)	524
PasI	CCCWGGG	1 cut(s)	1575
PcsI	WCGNNNNNNNCGW	6 cut(s)	1107, 1946, 1989, 2517, 2747, 2817
PctI	GAATGC	2 cut(s)	1569, 2842
PdiI	GCCGGC	1 cut(s)	1945
PfeI	GAWTC	6 cut(s)	207, 310, 384, 1200, 1251, 2914
PflMI	CCANNNNNTGG	2 cut(s)	578, 2422
PfoI	TCCNGGA	2 cut(s)	597, 1835
PinAI	ACCGGT	1 cut(s)	1778
PleI	GAGTC	2 cut(s)	507, 2617
PluTI	GGCGCC	1 cut(s)	1860
PpsI	GAGTC	2 cut(s)	507, 2617
PpuMI	RGGWCCY	1 cut(s)	1065
Psp124BI	GAGCTC	1 cut(s)	669
Psp5II	RGGWCCY	1 cut(s)	1065
Psp6I	CCWGG	5 cut(s)	427, 571, 1574, 1680, 2188
PspFI	CCCAGC	3 cut(s)	156, 375, 928
PspGI	CCWGG	5 cut(s)	427, 571, 1574, 1680, 2188
PspOMI	GGGCCC	1 cut(s)	1352
PspPPI	RGGWCCY	1 cut(s)	1065
PstI	CTGCAG	1 cut(s)	1464
PstNI	CAGNNNCTG	1 cut(s)	2343
PsuI	RGATCY	1 cut(s)	133
PvuII	CAGCTG	1 cut(s)	284
RsaI	GTAC	8 cut(s)	274, 717, 1237, 1394, 1711, 2366, 2447, 2854
RsaNI	GTAC	8 cut(s)	273, 716, 1236, 1393, 1710, 2365, 2446, 2853
RseI	CAYNNNNRTG	4 cut(s)	674, 2207, 2496, 2618
Rsr2I	CGGWCCG	2 cut(s)	2063, 2923
RsrII	CGGWCCG	2 cut(s)	2063, 2923
SacI	GAGCTC	1 cut(s)	669
SalI	GTCGAC	1 cut(s)	1109
SchI	GAGTC	2 cut(s)	507, 2618
SduI	GDGCHC	3 cut(s)	669, 851, 1356
SfaNI	GCATC	5 cut(s)	65, 1266, 2175, 2865, 2943
SfcI	CTRYAG	2 cut(s)	1460, 2029
SfoI	GGCGCC	1 cut(s)	1858
Sfr274I	CTCGAG	1 cut(s)	524
SfuI	TTCGAA	1 cut(s)	555
SgrAI	CRCCGGYG	1 cut(s)	2857
SinI	GGWCC	9 cut(s)	182, 488, 1065, 1136, 1684, 1781, 2063, 2160, 2923
SlaI	CTCGAG	1 cut(s)	524
SmiMI	CAYNNNNRTG	4 cut(s)	674, 2207, 2496, 2618
SmlI	CTYRAG	8 cut(s)	128, 322, 443, 451, 498, 524, 1609, 2525
SmoI	CTYRAG	8 cut(s)	128, 322, 443, 451, 498, 524, 1609, 2525
SspDI	GGCGCC	1 cut(s)	1856
SspI	AATATT	2 cut(s)	565, 586
SspMI	CTAG	3 cut(s)	233, 245, 2991
SstI	GAGCTC	1 cut(s)	669
StyI	CCWWGG	2 cut(s)	1646, 2470
TaaI	ACNGT	4 cut(s)	2067, 2170, 2498, 2552
TaiI	ACGT	3 cut(s)	1761, 1829, 2295
TaqII	GACCGA	1 cut(s)	1973
TatI	WGTACW	2 cut(s)	1235, 2364
TauI	GCSGC	6 cut(s)	935, 1382, 1857, 1883, 1970, 1973
TfiI	GAWTC	6 cut(s)	207, 310, 384, 1200, 1251, 2914
TscAI	CASTG	3 cut(s)	106, 490, 1595
TseFI	GTSAC	5 cut(s)	777, 1919, 2437, 2552, 2687
TseI	GCWGC	6 cut(s)	1518, 1566, 1927, 2328, 2331, 2353
Tsp45I	GTSAC	5 cut(s)	777, 1919, 2437, 2552, 2687
TspDTI	ATGAA	5 cut(s)	17, 186, 989, 1501, 1808
TspGWI	ACGGA	1 cut(s)	1779
TspRI	CASTG	3 cut(s)	106, 490, 1595
Van91I	CCANNNNNTGG	2 cut(s)	578, 2422
Vha464I	CTTAAG	1 cut(s)	1609
VpaK11BI	GGWCC	9 cut(s)	182, 488, 1065, 1136, 1684, 1781, 2063, 2160, 2923
XapI	RAATTY	7 cut(s)	79, 288, 1222, 1653, 1723, 2095, 2419
XbaI	TCTAGA	1 cut(s)	244
XceI	RCATGY	1 cut(s)	2371
XcmI	CCANNNNNNNNNTGG	3 cut(s)	1440, 1678, 2380
XhoI	CTCGAG	1 cut(s)	524
XmiI	GTMKAC	2 cut(s)	1110, 1530
XspI	CTAG	3 cut(s)	233, 245, 2991

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.