AT5G61530

Small G protein family protein RhoGAP

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0005451
Physical Location & Seq
Forward (+)
24741976 .. 24744823
2848 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G61530.3

Sequence Viewer

Length: 1131 bp
ATGCCTTCTCTCATCTCACAGCAATGGCAGGAGAGGACTAGTGGATTCTTTTCTTCATCAGGAACGAAGCTTAGGGAAGCAGGGCAGACTGCTGGTAGTTTTGTCGGGGAAGTTGCTAAGGATGCGAAAGTGAATGTTGCAGATGTTGCAGAAAGAGTTGGGTCGTTGTTTAAAAGCCGGTGGGCAATTCTTCAGCAGCCTGCAACTAGACATGCCGTGCAAGAACACCTCATAACAGCTGCTGCCACAACCGGGACTTTTGTCAGGAAGGGTATAACTGAGACAAAGGAAAAGGTTTCTGTTGGAAAGATCAAAGTGGAAGAGGCGGCCAAAAAGACTGCACAAAAGAGCAAGACCATTTTGACGGATATCGAAAGGTGGCAAAAGGGAGTTGCCAGCTCAGATGTGTTTGGTGTTGCGATTGAGATCACTGTCCAGCGGCAGGAGTCAAGCAGGCCTATTCCTCTGATACTAGTTAAATGCGCAGATTATCTCATATTAACAGGACTTAATTCACCGAACTTGTTCAAGGCCGAAGGAGATAGAAAGTTGATTCAACAATTGGTTTCTGCTTACAATCAAGATCCTAGGGCGTCAATACCTGAGGGTGTGAATCCAGTTGATGTCGCTGCACTCTTGAAATACTATCTTGCTAGCCTTCCGACACCACTAACTACTTTTGAGCTTTATAATGAAATCAAAGATGCACGGTCGAGCATACACAGAATGAGACAGTCACTCCAGAAGCTTTCCAATGTGAATTATAATACATTGGAGTTCATAACAGCGCTATTGCTTCGTGTGAGCCAGAAATCACTTCTTAACAAAATGGATTCGCATAGCCTAGCTATGGAAATGGCTCCAGTGATCATGTGGCGAGAAGACAACAGGCCTGAATCTTATCGGGAATACTGGAGACGCCCGTCAAGGAGTCCTAAGAAAAGTAACGACTTTGAAACTGCTACTCCATGGGACTTGCTCTCAGATGAAGGAGAAGGTCCAGATGCATCCTCATCAATCCCTCTAGATGATATCGCACGAGTTGATTTTGGAGCGGTCGAGGTTGTCCAGTGCCTAATTGAACACCACAATGCGATTTTCACTGATGCAGCCGAGACTGTATGGAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0008150 GO:0043087 GO:0044424 GO:0044464 GO:0050790 GO:0051336 GO:0065007 GO:0065009
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RhoGAP

Protein Analysis

376

Amino Acids

41.92

Weight (kDa)

7.73

Isoelectric Point (pI)

45.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RhoGAP PF00620 155 - 294 6.4e-34 RhoGAP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 690, 765
Acc16I TGCGCA 1 cut(s) 484
AccBSI CCGCTC 1 cut(s) 1055
AciI CCGC 3 cut(s) 326, 439, 1055
AclWI GGATC 1 cut(s) 578
AcoI YGGCCR 1 cut(s) 327
AcuI CTGAAG 1 cut(s) 176
AcyI GRCGYC 2 cut(s) 593, 919
AfeI AGCGCT 1 cut(s) 789
AfiI CCNNNNNNNGG 3 cut(s) 252, 442, 850
AgsI TTSAA 5 cut(s) 529, 557, 640, 956, 1082
AhlI ACTAGT 2 cut(s) 38, 472
AluBI AGCT 6 cut(s) 70, 239, 399, 685, 748, 848
AluI AGCT 6 cut(s) 70, 239, 399, 685, 748, 848
Alw26I GTCTC 4 cut(s) 275, 724, 910, 1109
AlwI GGATC 1 cut(s) 578
AlwNI CAGNNNCTG 1 cut(s) 242
Aor51HI AGCGCT 1 cut(s) 789
AoxI GGCC 4 cut(s) 327, 455, 531, 890
ApeKI GCWGC 5 cut(s) 196, 239, 242, 629, 1109
Asp700I GAANNNNTTC 1 cut(s) 524
AspA2I CCTAGG 1 cut(s) 587
AspLEI GCGC 2 cut(s) 485, 790
AspS9I GGNCC 1 cut(s) 998
AsuC2I CCSGG 1 cut(s) 253
AsuHPI GGTGA 1 cut(s) 507
AsuNHI GCTAGC 1 cut(s) 653
AvaII GGWCC 1 cut(s) 998
AvrII CCTAGG 1 cut(s) 587
AxyI CCTNAGG 1 cut(s) 603
BauI CACGAG 1 cut(s) 1038
BbsI GAAGAC 1 cut(s) 888
BbvI GCAGC 5 cut(s) 208, 226, 229, 616, 1121
BceAI ACGGC 1 cut(s) 200
BclI TGATCA 1 cut(s) 867
BcnI CCSGG 1 cut(s) 253
BcoDI GTCTC 4 cut(s) 275, 724, 910, 1109
BcuI ACTAGT 2 cut(s) 38, 472
BfaI CTAG 7 cut(s) 39, 207, 473, 588, 654, 845, 1025
BfoI RGCGCY 1 cut(s) 791
BisI GCNGC 7 cut(s) 197, 240, 243, 327, 440, 630, 1110
BlnI CCTAGG 1 cut(s) 587
BlsI GCNGC 7 cut(s) 198, 241, 244, 328, 441, 631, 1111
Bme1390I CCNGG 1 cut(s) 253
Bme18I GGWCC 1 cut(s) 998
BmgT120I GGNCC 1 cut(s) 998
BmiI GGNNCC 1 cut(s) 861
BmrFI CCNGG 1 cut(s) 253
BmsI GCATC 5 cut(s) 112, 694, 994, 1016, 1096
BmtI GCTAGC 1 cut(s) 657
BoxI GACNNNNGTC 2 cut(s) 260, 922
BpiI GAAGAC 1 cut(s) 888
BpmI CTGGAG 3 cut(s) 725, 846, 934
Bpu10I CCTNAGC 2 cut(s) 71, 117
BpuMI CCSGG 1 cut(s) 253
BsaBI GATNNNNATC 1 cut(s) 425
BsaHI GRCGYC 2 cut(s) 593, 919
BsaJI CCNNGG 2 cut(s) 587, 968
BsaXI ACNNNNNCTCC 4 cut(s) 723, 753, 949, 979
Bsc4I CCNNNNNNNGG 3 cut(s) 252, 442, 850
Bse118I RCCGGY 1 cut(s) 177
Bse1I ACTGG 4 cut(s) 617, 863, 917, 1069
Bse21I CCTNAGG 1 cut(s) 603
Bse3DI GCAATG 1 cut(s) 29
Bse8I GATNNNNATC 1 cut(s) 425
BseDI CCNNGG 2 cut(s) 587, 968
BseGI GGATG 2 cut(s) 127, 1007
BseJI GATNNNNATC 1 cut(s) 425
BseLI CCNNNNNNNGG 3 cut(s) 252, 442, 850
BseMI GCAATG 1 cut(s) 29
BseMII CTCAG 4 cut(s) 270, 414, 594, 996
BseNI ACTGG 4 cut(s) 617, 863, 917, 1069
BseXI GCAGC 5 cut(s) 208, 226, 229, 616, 1121
BsgI GTGCAG 2 cut(s) 324, 615
Bsh1285I CGRYCG 2 cut(s) 713, 1059
BshFI GGCC 4 cut(s) 329, 457, 533, 892
BsiEI CGRYCG 2 cut(s) 713, 1059
BsiSI CCGG 2 cut(s) 178, 252
BslFI GGGAC 2 cut(s) 268, 986
BslI CCNNNNNNNGG 3 cut(s) 252, 442, 850
BsmAI GTCTC 4 cut(s) 275, 724, 910, 1109
BsmBI CGTCTC 1 cut(s) 910
BsmFI GGGAC 2 cut(s) 268, 986
BsnI GGCC 4 cut(s) 329, 457, 533, 892
Bsp143I GATC 4 cut(s) 309, 426, 583, 867
Bsp19I CCATGG 1 cut(s) 968
BspACI CCGC 3 cut(s) 326, 439, 1055
BspANI GGCC 4 cut(s) 329, 457, 533, 892
BspCNI CTCAG 4 cut(s) 271, 413, 595, 995
BspLI GGNNCC 1 cut(s) 861
BspOI GCTAGC 1 cut(s) 657
BspPI GGATC 1 cut(s) 578
BsrBI CCGCTC 1 cut(s) 1055
BsrDI GCAATG 1 cut(s) 29
BsrFI RCCGGY 1 cut(s) 177
BsrI ACTGG 4 cut(s) 617, 863, 917, 1069
BssAI RCCGGY 1 cut(s) 177
BssECI CCNNGG 2 cut(s) 587, 968
BssMI GATC 4 cut(s) 309, 426, 583, 867
BssNI GRCGYC 2 cut(s) 593, 919
BssSI CACGAG 1 cut(s) 1038
BssT1I CCWWGG 2 cut(s) 587, 968
Bst2BI CACGAG 1 cut(s) 1038
Bst4CI ACNGT 4 cut(s) 433, 711, 735, 1120
Bst6I CTCTTC 1 cut(s) 315
BstACI GRCGYC 2 cut(s) 593, 919
BstAPI GCANNNNNTGC 1 cut(s) 146
BstC8I GCNNGC 4 cut(s) 201, 397, 455, 655
BstDEI CTNAG 7 cut(s) 71, 117, 279, 400, 603, 936, 982
BstDSI CCRYGG 1 cut(s) 968
BstF5I GGATG 2 cut(s) 127, 1007
BstH2I RGCGCY 1 cut(s) 791
BstHHI GCGC 2 cut(s) 485, 790
BstKTI GATC 4 cut(s) 312, 429, 586, 870
BstMAI GTCTC 4 cut(s) 275, 724, 910, 1109
BstMBI GATC 4 cut(s) 309, 426, 583, 867
BstMCI CGRYCG 2 cut(s) 713, 1059
BstMWI GCNNNNNNNGC 2 cut(s) 122, 146
BstNSI RCATGY 1 cut(s) 215
BstPAI GACNNNNGTC 2 cut(s) 260, 922
BstSCI CCNGG 1 cut(s) 251
BstV1I GCAGC 5 cut(s) 208, 226, 229, 616, 1121
BstV2I GAAGAC 1 cut(s) 888
BstX2I RGATCY 1 cut(s) 583
BstYI RGATCY 1 cut(s) 583
Bsu36I CCTNAGG 1 cut(s) 603
BsuRI GGCC 4 cut(s) 329, 457, 533, 892
BtgI CCRYGG 1 cut(s) 968
BtsCI GGATG 2 cut(s) 127, 1007
BtsIMutI CAGTG 4 cut(s) 429, 870, 1076, 1101
Cac8I GCNNGC 4 cut(s) 201, 397, 455, 655
CaiI CAGNNNCTG 1 cut(s) 242
CfoI GCGC 2 cut(s) 485, 790
Cfr10I RCCGGY 1 cut(s) 177
Cfr13I GGNCC 1 cut(s) 998
CseI GACGC 2 cut(s) 582, 927
CviAII CATG 3 cut(s) 212, 871, 969
DdeI CTNAG 7 cut(s) 71, 117, 279, 400, 603, 936, 982
DpnI GATC 4 cut(s) 311, 428, 585, 869
DpnII GATC 4 cut(s) 309, 426, 583, 867
DraI TTTAAA 1 cut(s) 172
EaeI YGGCCR 1 cut(s) 327
Eam1104I CTCTTC 1 cut(s) 315
EarI CTCTTC 1 cut(s) 315
Eco130I CCWWGG 2 cut(s) 587, 968
Eco147I AGGCCT 2 cut(s) 457, 892
Eco32I GATATC 2 cut(s) 370, 1033
Eco47I GGWCC 1 cut(s) 998
Eco47III AGCGCT 1 cut(s) 789
Eco57I CTGAAG 1 cut(s) 176
Eco81I CCTNAGG 1 cut(s) 603
EcoRV GATATC 2 cut(s) 370, 1033
EcoT14I CCWWGG 2 cut(s) 587, 968
EcoT22I ATGCAT 1 cut(s) 1009
ErhI CCWWGG 2 cut(s) 587, 968
Esp3I CGTCTC 1 cut(s) 910
FaeI CATG 3 cut(s) 215, 874, 972
FaqI GGGAC 2 cut(s) 268, 986
FatI CATG 3 cut(s) 211, 870, 968
FbaI TGATCA 1 cut(s) 867
Fnu4HI GCNGC 7 cut(s) 197, 240, 243, 327, 440, 630, 1110
FokI GGATG 2 cut(s) 134, 994
Fsp4HI GCNGC 7 cut(s) 197, 240, 243, 327, 440, 630, 1110
FspBI CTAG 7 cut(s) 39, 207, 473, 588, 654, 845, 1025
FspI TGCGCA 1 cut(s) 484
GlaI GCGC 2 cut(s) 484, 789
GluI GCNGC 7 cut(s) 197, 240, 243, 327, 440, 630, 1110
GsuI CTGGAG 3 cut(s) 725, 846, 934
HaeII RGCGCY 1 cut(s) 791
HaeIII GGCC 4 cut(s) 329, 457, 533, 892
HapII CCGG 2 cut(s) 178, 252
HgaI GACGC 2 cut(s) 582, 927
HhaI GCGC 2 cut(s) 485, 790
Hin1I GRCGYC 2 cut(s) 593, 919
Hin1II CATG 3 cut(s) 215, 874, 972
Hin6I GCGC 2 cut(s) 483, 788
HinP1I GCGC 2 cut(s) 483, 788
HindIII AAGCTT 2 cut(s) 68, 746
HinfI GANTC 7 cut(s) 45, 446, 553, 613, 833, 896, 931
HpaII CCGG 2 cut(s) 178, 252
HphI GGTGA 1 cut(s) 507
Hpy188I TCNGA 4 cut(s) 403, 468, 663, 985
Hpy188III TCNNGA 8 cut(s) 60, 265, 581, 637, 742, 905, 1001, 1025
HpyAV CCTTC 6 cut(s) 15, 262, 530, 668, 983, 989
HpyCH4III ACNGT 4 cut(s) 433, 711, 735, 1120
HpyCH4V TGCA 9 cut(s) 140, 149, 203, 220, 341, 632, 707, 1007, 1109
HpyF10VI GCNNNNNNNGC 2 cut(s) 122, 146
HpyF3I CTNAG 7 cut(s) 71, 117, 279, 400, 603, 936, 982
Hsp92I GRCGYC 2 cut(s) 593, 919
Hsp92II CATG 3 cut(s) 215, 874, 972
HspAI GCGC 2 cut(s) 483, 788
Ksp22I TGATCA 1 cut(s) 867
Kzo9I GATC 4 cut(s) 309, 426, 583, 867
LmnI GCTCC 2 cut(s) 865, 1052
Lsp1109I GCAGC 5 cut(s) 208, 226, 229, 616, 1121
LweI GCATC 5 cut(s) 112, 694, 994, 1016, 1096
MaeI CTAG 7 cut(s) 39, 207, 473, 588, 654, 845, 1025
MaeIII GTNAC 2 cut(s) 735, 944
MalI GATC 4 cut(s) 311, 428, 585, 869
MbiI CCGCTC 1 cut(s) 1055
MboI GATC 4 cut(s) 309, 426, 583, 867
MboII GAAGA 4 cut(s) 45, 182, 332, 893
MfeI CAATTG 1 cut(s) 560
MflI RGATCY 1 cut(s) 583
MluCI AATT 5 cut(s) 186, 511, 560, 760, 1077
MlyI GAGTC 2 cut(s) 455, 940
MmeI TCCRAC 2 cut(s) 283, 686
MnlI CCTC 9 cut(s) 27, 239, 316, 474, 598, 1021, 1032, 1054, 1119
Mph1103I ATGCAT 1 cut(s) 1009
MroXI GAANNNNTTC 1 cut(s) 524
MseI TTAA 5 cut(s) 171, 477, 500, 510, 822
MslI CAYNNNNRTG 2 cut(s) 22, 1089
MspA1I CMGCKG 2 cut(s) 239, 439
MspI CCGG 2 cut(s) 178, 252
MspR9I CCNGG 1 cut(s) 253
MunI CAATTG 1 cut(s) 560
MwoI GCNNNNNNNGC 2 cut(s) 122, 146
NciI CCSGG 1 cut(s) 253
NcoI CCATGG 1 cut(s) 968
NdeII GATC 4 cut(s) 309, 426, 583, 867
NheI GCTAGC 1 cut(s) 653
NlaIII CATG 3 cut(s) 215, 874, 972
NlaIV GGNNCC 1 cut(s) 861
NmuCI GTSAC 1 cut(s) 735
NsbI TGCGCA 1 cut(s) 484
NsiI ATGCAT 1 cut(s) 1009
NspI RCATGY 1 cut(s) 215
PceI AGGCCT 2 cut(s) 457, 892
PdmI GAANNNNTTC 1 cut(s) 524
PfeI GAWTC 5 cut(s) 45, 553, 613, 833, 896
PkrI GCNGC 7 cut(s) 198, 241, 244, 328, 441, 631, 1111
PleI GAGTC 2 cut(s) 454, 939
PpsI GAGTC 2 cut(s) 454, 939
PshAI GACNNNNGTC 2 cut(s) 260, 922
PsiI TTATAA 2 cut(s) 690, 765
PspN4I GGNNCC 1 cut(s) 861
PspPI GGNCC 1 cut(s) 998
PstNI CAGNNNCTG 1 cut(s) 242
PsuI RGATCY 1 cut(s) 583
PvuII CAGCTG 1 cut(s) 239
RseI CAYNNNNRTG 2 cut(s) 22, 1089
SaqAI TTAA 5 cut(s) 171, 477, 500, 510, 822
SatI GCNGC 7 cut(s) 197, 240, 243, 327, 440, 630, 1110
Sau3AI GATC 4 cut(s) 309, 426, 583, 867
Sau96I GGNCC 1 cut(s) 998
SchI GAGTC 2 cut(s) 455, 940
ScrFI CCNGG 1 cut(s) 253
SfaNI GCATC 5 cut(s) 112, 694, 994, 1016, 1096
SinI GGWCC 1 cut(s) 998
SmiMI CAYNNNNRTG 2 cut(s) 22, 1089
SpeI ACTAGT 2 cut(s) 38, 472
Sse9I AATT 5 cut(s) 186, 511, 560, 760, 1077
SseBI AGGCCT 2 cut(s) 457, 892
SsiI CCGC 3 cut(s) 326, 439, 1055
SspMI CTAG 7 cut(s) 39, 207, 473, 588, 654, 845, 1025
StuI AGGCCT 2 cut(s) 457, 892
StyD4I CCNGG 1 cut(s) 251
StyI CCWWGG 2 cut(s) 587, 968
TaaI ACNGT 4 cut(s) 433, 711, 735, 1120
TaqI TCGA 3 cut(s) 372, 713, 1059
TasI AATT 5 cut(s) 186, 511, 560, 760, 1077
TauI GCSGC 2 cut(s) 329, 442
TfiI GAWTC 5 cut(s) 45, 553, 613, 833, 896
Tru1I TTAA 5 cut(s) 171, 477, 500, 510, 822
Tru9I TTAA 5 cut(s) 171, 477, 500, 510, 822
TscAI CASTG 4 cut(s) 436, 870, 1076, 1108
TseFI GTSAC 1 cut(s) 735
TseI GCWGC 5 cut(s) 196, 239, 242, 629, 1109
Tsp45I GTSAC 1 cut(s) 735
TspDTI ATGAA 4 cut(s) 45, 708, 769, 1002
TspGWI ACGGA 1 cut(s) 380
TspRI CASTG 4 cut(s) 436, 870, 1076, 1108
VpaK11BI GGWCC 1 cut(s) 998
XbaI TCTAGA 1 cut(s) 1024
XceI RCATGY 1 cut(s) 215
XcmI CCANNNNNNNNNTGG 1 cut(s) 870
XmaJI CCTAGG 1 cut(s) 587
XmnI GAANNNNTTC 1 cut(s) 524
XspI CTAG 7 cut(s) 39, 207, 473, 588, 654, 845, 1025
Zsp2I ATGCAT 1 cut(s) 1009
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.