MD01G1178200.v1.1

Rho GTPase-activating protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0005451
Physical Location & Seq
Reverse (-)
27847067 .. 27850992
3926 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1178200.v1.1.491

Sequence Viewer

Length: 1122 bp
ATGGCTTTCCCGTTCTCACCCCCCATCACACAAGAGACAGCTACCGAATTCTTTTCTTCCTCAGGGGTGAAGATTAAAGAAGCTGGAACATTTGTTGGAGAGGTCACTAAAGATGCAAAAGTGAATGTTGCTGATGTGGCAGAACGAGTTGGGTCAATGTTCAAAAGTCGATGGGCGATTCTTCAGCAACCAGCTACAAGACATGCTGTGCAGGAACGCCTTATAACTGCTGCTGCAACAACTGGTACATTGTTTAGGAAGGGCTTATCAGAGACAAAGGAAAAGGTTTCTGTTGGGAAGATAAAAGTTGAGGAGGTGGCAAAGAAGACTGCCCAAAAGAGCAAAAGCATTTTGAGTGATATCGAAAGATGGCAGAAGGGTGTTGCAAGCACCGACGTTTTTGGAGTTCCGATTGAAGTTATTGTTCAACGGCAACAATCTAGTAGGCCCATTCCTCAAATTTTGGTCAGATGTGCAGATTATCTCATATTATCAGGACTAAATACACCATATCTATTTAAAGGCGAGGGAGATAGAAAAGTCATTCAACAACTGGTTTCACTGTACAACCAAGATTCAAATGCACCATTACCAGAGGGTGTAAACCCAATTGACGTAGCGGCTCTAGTCAAATGTTACCTAGCTACCCTTCCTGAGCCACTAACCACATTTGAGCTTTATAATGAGATCAAAGGTGCTCGATCCAGCATACATGCGATAAGAAACATGCTCAGGAGGCTTCCTACAGTCAACTACACAACCCTAGAATTTGTCACTGCATTACTGTTCCGTGTTAGCCAGAAGTCAGTTCTTAACAAGATGAGCACCCAAAGTCTCGCGATGGAGATGGCCCCTGTTATAATTTGGCAGAAGGGTAAAACACCGGACCTATATAGGAAATATTGGAATCAACTGTCAAAAGGTCCTTCCAGGAAGAACTTGGATCCTGAGCCTACTTATACTGCATGGGACATGCTTTCTGATGAAGGTGATGCTGTAGATGATTCCATCCCCTTGGATGATGACGTGCCCATGGACTTCGGCGCCATTGAGGTTGTCCAGTGCCTCATGGAACATCACAACGCAATTTTCACAGATGCTAATGAAACAATCTGGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0008150 GO:0043087 GO:0044424 GO:0044464 GO:0050790 GO:0051336 GO:0065007 GO:0065009
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RhoGAP

Protein Analysis

374

Amino Acids

41.6

Weight (kDa)

6.93

Isoelectric Point (pI)

42.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RhoGAP PF00620 152 - 291 6.7e-31 RhoGAP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 224, 681, 860
AccB1I GGYRCC 1 cut(s) 1043
AccII CGCG 1 cut(s) 839
AciI CCGC 1 cut(s) 620
AclWI GGATC 3 cut(s) 696, 938, 951
AcsI RAATTY 3 cut(s) 47, 459, 767
AcuI CTGAAG 1 cut(s) 167
AcyI GRCGYC 1 cut(s) 1044
AfaI GTAC 2 cut(s) 247, 566
AgsI TTSAA 5 cut(s) 163, 416, 428, 548, 579
AjiI CACGTC 1 cut(s) 1027
AjnI CCWGG 1 cut(s) 929
AluBI AGCT 5 cut(s) 41, 83, 194, 644, 676
AluI AGCT 5 cut(s) 41, 83, 194, 644, 676
Alw21I GWGCWC 2 cut(s) 700, 827
Alw26I GTCTC 3 cut(s) 29, 266, 839
AlwI GGATC 3 cut(s) 696, 938, 951
AoxI GGCC 2 cut(s) 446, 849
ApeKI GCWGC 2 cut(s) 230, 233
ApoI RAATTY 3 cut(s) 47, 459, 767
AspLEI GCGC 1 cut(s) 1046
AspS9I GGNCC 4 cut(s) 447, 850, 886, 923
AsuHPI GGTGA 3 cut(s) 9, 79, 1001
AvaII GGWCC 2 cut(s) 886, 923
AxyI CCTNAGG 1 cut(s) 61
BaeGI GKGCMC 1 cut(s) 1032
BamHI GGATCC 1 cut(s) 943
BanI GGYRCC 1 cut(s) 1043
BbsI GAAGAC 1 cut(s) 332
Bbv12I GWGCWC 2 cut(s) 700, 827
BbvI GCAGC 2 cut(s) 217, 220
BccI CCATC 6 cut(s) 32, 165, 363, 835, 841, 1016
BceAI ACGGC 1 cut(s) 446
BciT130I CCWGG 1 cut(s) 931
BcoDI GTCTC 3 cut(s) 29, 266, 839
BfaI CTAG 4 cut(s) 441, 626, 641, 764
BfmI CTRYAG 2 cut(s) 744, 996
BfoI RGCGCY 1 cut(s) 1047
BisI GCNGC 3 cut(s) 231, 234, 621
BlsI GCNGC 3 cut(s) 232, 235, 622
Bme1390I CCNGG 1 cut(s) 931
Bme18I GGWCC 2 cut(s) 886, 923
BmgBI CACGTC 1 cut(s) 1027
BmgT120I GGNCC 4 cut(s) 447, 850, 886, 923
BmiI GGNNCC 3 cut(s) 852, 945, 1045
BmrFI CCNGG 1 cut(s) 931
BmsI GCATC 3 cut(s) 103, 982, 1087
BpiI GAAGAC 1 cut(s) 332
Bpu10I CCTNAGC 3 cut(s) 654, 731, 948
BsaHI GRCGYC 1 cut(s) 1044
BsaJI CCNNGG 2 cut(s) 1014, 1032
BsaWI WCCGGW 1 cut(s) 883
Bse1I ACTGG 3 cut(s) 247, 558, 1060
Bse21I CCTNAGG 1 cut(s) 61
BseBI CCWGG 1 cut(s) 931
BseDI CCNNGG 2 cut(s) 1014, 1032
BseGI GGATG 2 cut(s) 1008, 1024
BseMII CTCAG 4 cut(s) 75, 645, 745, 939
BseNI ACTGG 3 cut(s) 247, 558, 1060
BseRI GAGGAG 1 cut(s) 326
BseSI GKGCMC 1 cut(s) 1032
BseXI GCAGC 2 cut(s) 217, 220
BsgI GTGCAG 2 cut(s) 230, 495
Bsh1236I CGCG 1 cut(s) 839
BshFI GGCC 2 cut(s) 448, 851
BshNI GGYRCC 1 cut(s) 1043
BsiHKAI GWGCWC 2 cut(s) 700, 827
BsiSI CCGG 1 cut(s) 884
BslFI GGGAC 1 cut(s) 983
BsmAI GTCTC 3 cut(s) 29, 266, 839
BsmFI GGGAC 1 cut(s) 983
BsnI GGCC 2 cut(s) 448, 851
Bsp1286I GDGCHC 3 cut(s) 700, 827, 1032
Bsp1407I TGTACA 1 cut(s) 564
Bsp143I GATC 3 cut(s) 687, 701, 943
Bsp19I CCATGG 1 cut(s) 1032
Bsp68I TCGCGA 1 cut(s) 839
BspACI CCGC 1 cut(s) 620
BspANI GGCC 2 cut(s) 448, 851
BspCNI CTCAG 4 cut(s) 74, 646, 744, 940
BspFNI CGCG 1 cut(s) 839
BspLI GGNNCC 3 cut(s) 852, 945, 1045
BspPI GGATC 3 cut(s) 696, 938, 951
BspT107I GGYRCC 1 cut(s) 1043
BsrGI TGTACA 1 cut(s) 564
BsrI ACTGG 3 cut(s) 247, 558, 1060
BssECI CCNNGG 2 cut(s) 1014, 1032
BssMI GATC 3 cut(s) 687, 701, 943
BssNI GRCGYC 1 cut(s) 1044
BssT1I CCWWGG 2 cut(s) 1014, 1032
Bst2UI CCWGG 1 cut(s) 931
Bst4CI ACNGT 4 cut(s) 564, 748, 786, 915
BstACI GRCGYC 1 cut(s) 1044
BstAUI TGTACA 1 cut(s) 564
BstC8I GCNNGC 1 cut(s) 388
BstDEI CTNAG 4 cut(s) 61, 654, 731, 948
BstDSI CCRYGG 1 cut(s) 1032
BstF5I GGATG 2 cut(s) 1008, 1024
BstFNI CGCG 1 cut(s) 839
BstH2I RGCGCY 1 cut(s) 1047
BstHHI GCGC 1 cut(s) 1046
BstKTI GATC 3 cut(s) 690, 704, 946
BstMAI GTCTC 3 cut(s) 29, 266, 839
BstMBI GATC 3 cut(s) 687, 701, 943
BstMWI GCNNNNNNNGC 2 cut(s) 137, 736
BstNI CCWGG 1 cut(s) 931
BstNSI RCATGY 4 cut(s) 206, 716, 730, 976
BstSCI CCNGG 1 cut(s) 929
BstSFI CTRYAG 2 cut(s) 744, 996
BstSLI GKGCMC 1 cut(s) 1032
BstUI CGCG 1 cut(s) 839
BstV1I GCAGC 2 cut(s) 217, 220
BstV2I GAAGAC 1 cut(s) 332
BstX2I RGATCY 1 cut(s) 943
BstXI CCANNNNNNTGG 1 cut(s) 1015
BstYI RGATCY 1 cut(s) 943
Bsu36I CCTNAGG 1 cut(s) 61
BsuRI GGCC 2 cut(s) 448, 851
BtgI CCRYGG 1 cut(s) 1032
BtgZI GCGATG 1 cut(s) 854
BtrI CACGTC 1 cut(s) 1027
BtsCI GGATG 2 cut(s) 1008, 1024
BtsI GCAGTG 1 cut(s) 774
BtsIMutI CAGTG 3 cut(s) 560, 774, 1067
BtuMI TCGCGA 1 cut(s) 839
Cac8I GCNNGC 1 cut(s) 388
CfoI GCGC 1 cut(s) 1046
Cfr13I GGNCC 4 cut(s) 447, 850, 886, 923
Csp6I GTAC 2 cut(s) 246, 565
CviAII CATG 7 cut(s) 203, 713, 727, 966, 973, 1033, 1069
CviQI GTAC 2 cut(s) 246, 565
DdeI CTNAG 4 cut(s) 61, 654, 731, 948
DinI GGCGCC 1 cut(s) 1045
DpnI GATC 3 cut(s) 689, 703, 945
DpnII GATC 3 cut(s) 687, 701, 943
DraI TTTAAA 1 cut(s) 520
Eco130I CCWWGG 2 cut(s) 1014, 1032
Eco32I GATATC 1 cut(s) 361
Eco47I GGWCC 2 cut(s) 886, 923
Eco57I CTGAAG 1 cut(s) 167
Eco81I CCTNAGG 1 cut(s) 61
EcoO109I RGGNCCY 1 cut(s) 923
EcoRI GAATTC 1 cut(s) 47
EcoRII CCWGG 1 cut(s) 929
EcoRV GATATC 1 cut(s) 361
EcoT14I CCWWGG 2 cut(s) 1014, 1032
EgeI GGCGCC 1 cut(s) 1045
EheI GGCGCC 1 cut(s) 1045
ErhI CCWWGG 2 cut(s) 1014, 1032
FaeI CATG 7 cut(s) 206, 716, 730, 969, 976, 1036, 1072
FaqI GGGAC 1 cut(s) 983
FatI CATG 7 cut(s) 202, 712, 726, 965, 972, 1032, 1068
Fnu4HI GCNGC 3 cut(s) 231, 234, 621
FokI GGATG 2 cut(s) 995, 1031
Fsp4HI GCNGC 3 cut(s) 231, 234, 621
FspBI CTAG 4 cut(s) 441, 626, 641, 764
GlaI GCGC 1 cut(s) 1045
GluI GCNGC 3 cut(s) 231, 234, 621
HaeII RGCGCY 1 cut(s) 1047
HaeIII GGCC 2 cut(s) 448, 851
HapII CCGG 1 cut(s) 884
HhaI GCGC 1 cut(s) 1046
Hin1I GRCGYC 1 cut(s) 1044
Hin1II CATG 7 cut(s) 206, 716, 730, 969, 976, 1036, 1072
Hin6I GCGC 1 cut(s) 1044
HinP1I GCGC 1 cut(s) 1044
HincII GTYRAC 1 cut(s) 751
HindII GTYRAC 1 cut(s) 751
HinfI GANTC 4 cut(s) 178, 575, 907, 1004
HpaII CCGG 1 cut(s) 884
HphI GGTGA 3 cut(s) 9, 79, 1001
Hpy166II GTNNAC 2 cut(s) 604, 751
Hpy188I TCNGA 4 cut(s) 271, 411, 470, 982
Hpy188III TCNNGA 6 cut(s) 495, 653, 733, 838, 947, 1114
Hpy8I GTNNAC 2 cut(s) 604, 751
Hpy99I CGWCG 1 cut(s) 398
HpyAV CCTTC 6 cut(s) 253, 370, 659, 865, 936, 980
HpyCH4III ACNGT 4 cut(s) 564, 748, 786, 915
HpyCH4IV ACGT 3 cut(s) 396, 615, 1026
HpyCH4V TGCA 8 cut(s) 116, 211, 236, 386, 476, 584, 779, 965
HpyF10VI GCNNNNNNNGC 2 cut(s) 137, 736
HpyF3I CTNAG 4 cut(s) 61, 654, 731, 948
HpySE526I ACGT 3 cut(s) 396, 615, 1026
Hsp92I GRCGYC 1 cut(s) 1044
Hsp92II CATG 7 cut(s) 206, 716, 730, 969, 976, 1036, 1072
HspAI GCGC 1 cut(s) 1044
KasI GGCGCC 1 cut(s) 1043
Kzo9I GATC 3 cut(s) 687, 701, 943
Lsp1109I GCAGC 2 cut(s) 217, 220
LweI GCATC 3 cut(s) 103, 982, 1087
MaeI CTAG 4 cut(s) 441, 626, 641, 764
MaeII ACGT 3 cut(s) 396, 615, 1026
MaeIII GTNAC 3 cut(s) 103, 635, 772
MalI GATC 3 cut(s) 689, 703, 945
MboI GATC 3 cut(s) 687, 701, 943
MboII GAAGA 6 cut(s) 48, 82, 173, 310, 337, 946
MfeI CAATTG 1 cut(s) 609
MflI RGATCY 1 cut(s) 943
MhlI GDGCHC 3 cut(s) 700, 827, 1032
MluCI AATT 6 cut(s) 47, 459, 609, 767, 861, 1086
Mly113I GGCGCC 1 cut(s) 1044
MmeI TCCRAC 1 cut(s) 76
MseI TTAA 3 cut(s) 75, 519, 813
MspI CCGG 1 cut(s) 884
MspR9I CCNGG 1 cut(s) 931
MunI CAATTG 1 cut(s) 609
MvaI CCWGG 1 cut(s) 931
MvnI CGCG 1 cut(s) 839
MwoI GCNNNNNNNGC 2 cut(s) 137, 736
NarI GGCGCC 1 cut(s) 1044
NcoI CCATGG 1 cut(s) 1032
NdeII GATC 3 cut(s) 687, 701, 943
NlaIII CATG 7 cut(s) 206, 716, 730, 969, 976, 1036, 1072
NlaIV GGNNCC 3 cut(s) 852, 945, 1045
NmuCI GTSAC 2 cut(s) 103, 772
NruI TCGCGA 1 cut(s) 839
NspI RCATGY 4 cut(s) 206, 716, 730, 976
PfeI GAWTC 4 cut(s) 178, 575, 907, 1004
PfoI TCCNGGA 1 cut(s) 929
PkrI GCNGC 3 cut(s) 232, 235, 622
PluTI GGCGCC 1 cut(s) 1047
PpuMI RGGWCCY 1 cut(s) 923
PsiI TTATAA 3 cut(s) 224, 681, 860
Psp5II RGGWCCY 1 cut(s) 923
Psp6I CCWGG 1 cut(s) 929
PspGI CCWGG 1 cut(s) 929
PspN4I GGNNCC 3 cut(s) 852, 945, 1045
PspPI GGNCC 4 cut(s) 447, 850, 886, 923
PspPPI RGGWCCY 1 cut(s) 923
PsuI RGATCY 1 cut(s) 943
RruI TCGCGA 1 cut(s) 839
RsaI GTAC 2 cut(s) 247, 566
RsaNI GTAC 2 cut(s) 246, 565
SaqAI TTAA 3 cut(s) 75, 519, 813
SatI GCNGC 3 cut(s) 231, 234, 621
Sau3AI GATC 3 cut(s) 687, 701, 943
Sau96I GGNCC 4 cut(s) 447, 850, 886, 923
ScrFI CCNGG 1 cut(s) 931
SduI GDGCHC 3 cut(s) 700, 827, 1032
SfaNI GCATC 3 cut(s) 103, 982, 1087
SfcI CTRYAG 2 cut(s) 744, 996
SfoI GGCGCC 1 cut(s) 1045
SinI GGWCC 2 cut(s) 886, 923
Sse9I AATT 6 cut(s) 47, 459, 609, 767, 861, 1086
SsiI CCGC 1 cut(s) 620
SspDI GGCGCC 1 cut(s) 1043
SspI AATATT 1 cut(s) 902
SspMI CTAG 4 cut(s) 441, 626, 641, 764
StyD4I CCNGG 1 cut(s) 929
StyI CCWWGG 2 cut(s) 1014, 1032
TaaI ACNGT 4 cut(s) 564, 748, 786, 915
TaiI ACGT 3 cut(s) 399, 618, 1029
TaqI TCGA 3 cut(s) 169, 363, 700
TasI AATT 6 cut(s) 47, 459, 609, 767, 861, 1086
TatI WGTACW 1 cut(s) 564
TauI GCSGC 1 cut(s) 623
TfiI GAWTC 4 cut(s) 178, 575, 907, 1004
Tru1I TTAA 3 cut(s) 75, 519, 813
Tru9I TTAA 3 cut(s) 75, 519, 813
TscAI CASTG 3 cut(s) 567, 781, 1067
TseFI GTSAC 2 cut(s) 103, 772
TseI GCWGC 2 cut(s) 230, 233
Tsp45I GTSAC 2 cut(s) 103, 772
TspDTI ATGAA 2 cut(s) 999, 1119
TspGWI ACGGA 1 cut(s) 779
TspRI CASTG 3 cut(s) 567, 781, 1067
VpaK11BI GGWCC 2 cut(s) 886, 923
XapI RAATTY 3 cut(s) 47, 459, 767
XceI RCATGY 4 cut(s) 206, 716, 730, 976
XcmI CCANNNNNNNNNTGG 1 cut(s) 937
XspI CTAG 4 cut(s) 441, 626, 641, 764
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.