RchiOBHm_Chr1g0373901

Rho GTPase-activating protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0005451
Physical Location & Seq
Reverse (-)
61885446 .. 61889826
4381 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ59773

Sequence Viewer

Length: 1131 bp
ATGCCTTCGGCTGTCTCGCCGCGGTGGCAAGAGAAGGCTACTGGTTTCTTTTCTACCTCAGGGGTGAAGATTAAGGAAGCTGGACAAACGGCTGGGACATTTGTTGAGGAGGTTACGAAGGATGCTAAAGTTAATGTGTCTGATATGGCAGGGCGAGTTGGGTCAATGTTCAAGAGTCGATGGGCGATTCTTCAGCAGCCATCGACTAGACAAGCTGTGCAGGAGCGCCTTATAACTGCTGCTGCTACGACTGGCACATTGTTCAGGAAGGGATTGTCGGAGACAAAGGACAAGGTTTCGGTTGGGAAGATAAAAGTTGAAGAGGTGGCAAAGAAGACTGCCCAAAAAAGCAAAACCATTCTGACTGACATTGAAAGATGGCAGAAGGGTGTTGCAAGCACAGATGTTTTTGGAGTTCCGATTGAAGTTACTGTGCAACGGCAACAATCTAGCAGGCCTATTCCTCATATTTTGGTGAACTGTGCAGATTATCTCATATTATCAGGGCTAAACACACCATACCTGTTTAAAGGTGAGGGAGATAAAAAAGTTATCCAACAATTGATTTCGATGTATAACCAAGATTCAAATGCATCAATACCAGAGGGTGTAAGTTCAATTGATGTAGCGGCTCTTGTCAAATGTTACCTAGCTACCCTTCCTGAGCCACTAACCACACTTGAGCTTCATAACGAGATCAGAGGTGCTCGTTCTAGCATAAATGCAATGAGAAACACGCTGAAGAAGCTTCCTACTGTAAACTATACAACACTGGAATTTATTACTGCACTACTGCTGCGTGTTAGCCAGAAGTCAGTTCTTAACAAGATGGACACCCAAAGTCTTGCAATGGAGATGGCCCCGGTTATAATTTGGCAGAAGGGTCGGGCACCAGACCTATATAAAAAGTATTGGAATCAACCATCTAAAGGTCTTTCCAAGAAAAACTCAGATCCAGAGCCTACTTATAGTGCATGGGACATGCTTTCTGATGAAGGTGATACTGTAGATGATTCCATTCCCTTGGATGATGGAGTGGCTATTGACTTGGGAGCTATCGAGGTTGTTCAGTGTCTCATGGAACATCACAATGCGATTTTCACTGATGCAAATGAAACAGTCTGGAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0008150 GO:0043087 GO:0044424 GO:0044464 GO:0050790 GO:0051336 GO:0065007 GO:0065009
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RhoGAP

Protein Analysis

376

Amino Acids

41.5

Weight (kDa)

8.23

Isoelectric Point (pI)

41.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RhoGAP PF00620 155 - 294 7.6e-30 RhoGAP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 233, 869
AccB1I GGYRCC 1 cut(s) 889
AccII CGCG 1 cut(s) 22
AciI CCGC 3 cut(s) 20, 22, 629
AclWI GGATC 1 cut(s) 947
AcsI RAATTY 1 cut(s) 776
AcuI CTGAAG 2 cut(s) 176, 761
AfiI CCNNNNNNNGG 1 cut(s) 929
AgsI TTSAA 6 cut(s) 172, 320, 374, 425, 588, 618
AluBI AGCT 6 cut(s) 80, 215, 653, 685, 748, 1055
AluI AGCT 6 cut(s) 80, 215, 653, 685, 748, 1055
Alw21I GWGCWC 1 cut(s) 709
Alw26I GTCTC 3 cut(s) 19, 275, 1079
AlwI GGATC 1 cut(s) 947
AoxI GGCC 2 cut(s) 455, 858
ApeKI GCWGC 4 cut(s) 196, 239, 242, 796
ApoI RAATTY 1 cut(s) 776
AspLEI GCGC 1 cut(s) 228
AspS9I GGNCC 1 cut(s) 859
AsuC2I CCSGG 1 cut(s) 863
AsuHPI GGTGA 4 cut(s) 76, 487, 545, 1010
AxyI CCTNAGG 1 cut(s) 58
BaeGI GKGCMC 1 cut(s) 892
BanI GGYRCC 1 cut(s) 889
BbsI GAAGAC 1 cut(s) 341
Bbv12I GWGCWC 1 cut(s) 709
BbvI GCAGC 4 cut(s) 208, 226, 229, 783
BccI CCATC 7 cut(s) 174, 208, 372, 823, 850, 931, 1025
BceAI ACGGC 2 cut(s) 105, 455
BcgI CGANNNNNNTGC 2 cut(s) 866, 900
BcnI CCSGG 1 cut(s) 863
BcoDI GTCTC 3 cut(s) 19, 275, 1079
BfaI CTAG 4 cut(s) 207, 450, 650, 714
BfmI CTRYAG 1 cut(s) 1005
BfoI RGCGCY 1 cut(s) 229
BglI GCCNNNNNGGC 1 cut(s) 25
BisI GCNGC 6 cut(s) 20, 197, 240, 243, 630, 797
BlsI GCNGC 6 cut(s) 21, 198, 241, 244, 631, 798
Bme1390I CCNGG 1 cut(s) 863
BmgT120I GGNCC 1 cut(s) 859
BmiI GGNNCC 2 cut(s) 861, 891
BmrFI CCNGG 1 cut(s) 863
BmsI GCATC 3 cut(s) 112, 602, 1096
BpiI GAAGAC 1 cut(s) 341
Bpu10I CCTNAGC 1 cut(s) 663
BpuEI CTTGAG 1 cut(s) 701
BpuMI CCSGG 1 cut(s) 863
BsaJI CCNNGG 3 cut(s) 20, 861, 1023
Bsc4I CCNNNNNNNGG 1 cut(s) 929
Bse1I ACTGG 3 cut(s) 46, 256, 777
Bse21I CCTNAGG 1 cut(s) 58
Bse3DI GCAATG 2 cut(s) 732, 855
BseDI CCNNGG 3 cut(s) 20, 861, 1023
BseGI GGATG 2 cut(s) 127, 1033
BseLI CCNNNNNNNGG 1 cut(s) 929
BseMI GCAATG 2 cut(s) 732, 855
BseMII CTCAG 3 cut(s) 72, 654, 963
BseNI ACTGG 3 cut(s) 46, 256, 777
BseRI GAGGAG 1 cut(s) 122
BseSI GKGCMC 1 cut(s) 892
BseXI GCAGC 4 cut(s) 208, 226, 229, 783
BseYI CCCAGC 1 cut(s) 92
BsgI GTGCAG 3 cut(s) 239, 504, 771
Bsh1236I CGCG 1 cut(s) 22
BshFI GGCC 2 cut(s) 457, 860
BshNI GGYRCC 1 cut(s) 889
BsiHKAI GWGCWC 1 cut(s) 709
BsiSI CCGG 1 cut(s) 863
BslFI GGGAC 2 cut(s) 109, 992
BslI CCNNNNNNNGG 1 cut(s) 929
BsmAI GTCTC 3 cut(s) 19, 275, 1079
BsmFI GGGAC 2 cut(s) 109, 992
BsnI GGCC 2 cut(s) 457, 860
Bsp1286I GDGCHC 2 cut(s) 709, 892
Bsp143I GATC 2 cut(s) 696, 952
BspACI CCGC 3 cut(s) 20, 22, 629
BspANI GGCC 2 cut(s) 457, 860
BspCNI CTCAG 3 cut(s) 71, 655, 962
BspFNI CGCG 1 cut(s) 22
BspLI GGNNCC 2 cut(s) 861, 891
BspPI GGATC 1 cut(s) 947
BspT107I GGYRCC 1 cut(s) 889
BsrDI GCAATG 2 cut(s) 732, 855
BsrI ACTGG 3 cut(s) 46, 256, 777
BssECI CCNNGG 3 cut(s) 20, 861, 1023
BssMI GATC 2 cut(s) 696, 952
BssT1I CCWWGG 1 cut(s) 1023
Bst4CI ACNGT 5 cut(s) 433, 482, 757, 1006, 1120
Bst6I CTCTTC 1 cut(s) 315
BstC8I GCNNGC 2 cut(s) 397, 455
BstDEI CTNAG 3 cut(s) 58, 663, 949
BstDSI CCRYGG 1 cut(s) 20
BstF5I GGATG 2 cut(s) 127, 1033
BstFNI CGCG 1 cut(s) 22
BstH2I RGCGCY 1 cut(s) 229
BstHHI GCGC 1 cut(s) 228
BstKTI GATC 2 cut(s) 699, 955
BstMAI GTCTC 3 cut(s) 19, 275, 1079
BstMBI GATC 2 cut(s) 696, 952
BstMWI GCNNNNNNNGC 2 cut(s) 25, 745
BstNSI RCATGY 1 cut(s) 985
BstSCI CCNGG 1 cut(s) 861
BstSFI CTRYAG 1 cut(s) 1005
BstSLI GKGCMC 1 cut(s) 892
BstUI CGCG 1 cut(s) 22
BstV1I GCAGC 4 cut(s) 208, 226, 229, 783
BstV2I GAAGAC 1 cut(s) 341
BstX2I RGATCY 1 cut(s) 952
BstXI CCANNNNNNTGG 1 cut(s) 1024
BstYI RGATCY 1 cut(s) 952
Bsu36I CCTNAGG 1 cut(s) 58
BsuRI GGCC 2 cut(s) 457, 860
BtgI CCRYGG 1 cut(s) 20
BtsCI GGATG 2 cut(s) 127, 1033
BtsIMutI CAGTG 3 cut(s) 770, 1076, 1101
Cac8I GCNNGC 2 cut(s) 397, 455
CfoI GCGC 1 cut(s) 228
Cfr13I GGNCC 1 cut(s) 859
Cfr42I CCGCGG 1 cut(s) 23
CviAII CATG 3 cut(s) 975, 982, 1078
DdeI CTNAG 3 cut(s) 58, 663, 949
DpnI GATC 2 cut(s) 698, 954
DpnII GATC 2 cut(s) 696, 952
DraI TTTAAA 1 cut(s) 529
Eam1104I CTCTTC 1 cut(s) 315
EarI CTCTTC 1 cut(s) 315
Eco130I CCWWGG 1 cut(s) 1023
Eco147I AGGCCT 1 cut(s) 457
Eco57I CTGAAG 2 cut(s) 176, 761
Eco81I CCTNAGG 1 cut(s) 58
EcoT14I CCWWGG 1 cut(s) 1023
EcoT22I ATGCAT 1 cut(s) 595
ErhI CCWWGG 1 cut(s) 1023
FaeI CATG 3 cut(s) 978, 985, 1081
FaqI GGGAC 2 cut(s) 109, 992
FatI CATG 3 cut(s) 974, 981, 1077
Fnu4HI GCNGC 6 cut(s) 20, 197, 240, 243, 630, 797
FokI GGATG 2 cut(s) 134, 1040
Fsp4HI GCNGC 6 cut(s) 20, 197, 240, 243, 630, 797
FspBI CTAG 4 cut(s) 207, 450, 650, 714
GlaI GCGC 1 cut(s) 227
GluI GCNGC 6 cut(s) 20, 197, 240, 243, 630, 797
GsaI CCCAGC 1 cut(s) 96
HaeII RGCGCY 1 cut(s) 229
HaeIII GGCC 2 cut(s) 457, 860
HapII CCGG 1 cut(s) 863
HhaI GCGC 1 cut(s) 228
Hin1II CATG 3 cut(s) 978, 985, 1081
Hin6I GCGC 1 cut(s) 226
HinP1I GCGC 1 cut(s) 226
HindIII AAGCTT 1 cut(s) 746
HinfI GANTC 5 cut(s) 175, 187, 584, 916, 1013
HpaII CCGG 1 cut(s) 863
HphI GGTGA 4 cut(s) 76, 487, 545, 1010
Hpy166II GTNNAC 2 cut(s) 478, 760
Hpy188I TCNGA 7 cut(s) 142, 280, 363, 420, 701, 952, 991
Hpy188III TCNNGA 5 cut(s) 172, 265, 662, 956, 1123
Hpy8I GTNNAC 2 cut(s) 478, 760
HpyAV CCTTC 8 cut(s) 15, 28, 112, 262, 379, 668, 874, 989
HpyCH4III ACNGT 5 cut(s) 433, 482, 757, 1006, 1120
HpyF10VI GCNNNNNNNGC 2 cut(s) 25, 745
HpyF3I CTNAG 3 cut(s) 58, 663, 949
Hsp92II CATG 3 cut(s) 978, 985, 1081
HspAI GCGC 1 cut(s) 226
KspI CCGCGG 1 cut(s) 23
Kzo9I GATC 2 cut(s) 696, 952
LmnI GCTCC 2 cut(s) 223, 1052
Lsp1109I GCAGC 4 cut(s) 208, 226, 229, 783
LweI GCATC 3 cut(s) 112, 602, 1096
MaeI CTAG 4 cut(s) 207, 450, 650, 714
MaeIII GTNAC 3 cut(s) 112, 427, 644
MalI GATC 2 cut(s) 698, 954
MboI GATC 2 cut(s) 696, 952
MboII GAAGA 6 cut(s) 79, 182, 319, 332, 346, 754
MfeI CAATTG 2 cut(s) 560, 618
MflI RGATCY 1 cut(s) 952
MhlI GDGCHC 2 cut(s) 709, 892
MluCI AATT 4 cut(s) 560, 618, 776, 870
MlyI GAGTC 1 cut(s) 184
MmeI TCCRAC 2 cut(s) 258, 580
Mph1103I ATGCAT 1 cut(s) 595
MseI TTAA 4 cut(s) 72, 132, 528, 822
MslI CAYNNNNRTG 1 cut(s) 1089
MspA1I CMGCKG 1 cut(s) 22
MspI CCGG 1 cut(s) 863
MspR9I CCNGG 1 cut(s) 863
MunI CAATTG 2 cut(s) 560, 618
MvnI CGCG 1 cut(s) 22
MwoI GCNNNNNNNGC 2 cut(s) 25, 745
NciI CCSGG 1 cut(s) 863
NdeII GATC 2 cut(s) 696, 952
NlaIII CATG 3 cut(s) 978, 985, 1081
NlaIV GGNNCC 2 cut(s) 861, 891
NsiI ATGCAT 1 cut(s) 595
NspI RCATGY 1 cut(s) 985
PceI AGGCCT 1 cut(s) 457
PfeI GAWTC 4 cut(s) 187, 584, 916, 1013
PkrI GCNGC 6 cut(s) 21, 198, 241, 244, 631, 798
PleI GAGTC 1 cut(s) 183
PpsI GAGTC 1 cut(s) 183
PsiI TTATAA 2 cut(s) 233, 869
PspFI CCCAGC 1 cut(s) 92
PspN4I GGNNCC 2 cut(s) 861, 891
PspPI GGNCC 1 cut(s) 859
PsuI RGATCY 1 cut(s) 952
RseI CAYNNNNRTG 1 cut(s) 1089
SacII CCGCGG 1 cut(s) 23
SaqAI TTAA 4 cut(s) 72, 132, 528, 822
SatI GCNGC 6 cut(s) 20, 197, 240, 243, 630, 797
Sau3AI GATC 2 cut(s) 696, 952
Sau96I GGNCC 1 cut(s) 859
SchI GAGTC 1 cut(s) 184
ScrFI CCNGG 1 cut(s) 863
SduI GDGCHC 2 cut(s) 709, 892
SfaNI GCATC 3 cut(s) 112, 602, 1096
SfcI CTRYAG 1 cut(s) 1005
Sfr303I CCGCGG 1 cut(s) 23
SgrBI CCGCGG 1 cut(s) 23
SmiMI CAYNNNNRTG 1 cut(s) 1089
SmlI CTYRAG 1 cut(s) 680
SmoI CTYRAG 1 cut(s) 680
Sse9I AATT 4 cut(s) 560, 618, 776, 870
SseBI AGGCCT 1 cut(s) 457
SsiI CCGC 3 cut(s) 20, 22, 629
SspMI CTAG 4 cut(s) 207, 450, 650, 714
StuI AGGCCT 1 cut(s) 457
StyD4I CCNGG 1 cut(s) 861
StyI CCWWGG 1 cut(s) 1023
TaaI ACNGT 5 cut(s) 433, 482, 757, 1006, 1120
TaqI TCGA 4 cut(s) 178, 203, 569, 1059
TasI AATT 4 cut(s) 560, 618, 776, 870
TauI GCSGC 2 cut(s) 22, 632
TfiI GAWTC 4 cut(s) 187, 584, 916, 1013
Tru1I TTAA 4 cut(s) 72, 132, 528, 822
Tru9I TTAA 4 cut(s) 72, 132, 528, 822
TscAI CASTG 3 cut(s) 777, 1076, 1108
TseI GCWGC 4 cut(s) 196, 239, 242, 796
TspDTI ATGAA 3 cut(s) 677, 1008, 1128
TspRI CASTG 3 cut(s) 777, 1076, 1108
XapI RAATTY 1 cut(s) 776
XceI RCATGY 1 cut(s) 985
XspI CTAG 4 cut(s) 207, 450, 650, 714
Zsp2I ATGCAT 1 cut(s) 595
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.