RLG00000026797

Rho GTPase-activating protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0005451
Physical Location & Seq
Forward (+)
6105437 .. 6110294
4858 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000026797

Sequence Viewer

Length: 1365 bp
ATGACTGGGGACGAATCGGACCGGCAGATCTTGAACTCGATATCTGACAGGCAGGTCTTCAACCTTGTGGCCGACGAGCTCGTCTCCCGCGGCTTCTTCCTCCACCGCACCACCGCCGACCTCAACCTCAACCACCTTGAGTCTTCGTCGCCGTCGGGTTACCCAAGGAAGACCCGGCCAAGATTCGGATCGAGAAACATATCTGAGTTGTCCCAAAATCCGTCGGAAAGCTTCATCTTCTTCTTCTTTCCTGATCAAAGTTCAGACCAGGCTACTGGTTTCTTTTCTACCTCAGGGGTGAAGATTCAGGAAGCTGGACAAACGGCTGGGACATTTGTTGAGGAGGTTACTAAGGATGCTAAAGTTAATGTGTCTGATATGGCAGGGCGAGTTGGGTCAATGTTCAAGAGCCGATGGGCGATTCTTCAGCAGCCATCAACTAGACAAGCTGTACAGGAGCGCCTTCTAACTGCTGTTGCTACGACTGGCACATTGTTTAGGAAGGGATTGTCGGAGACAAAGGACAAGGTTTCGGTTGGGAAGATAAAAGTTGAAGAGGTGGCAAAGAAGACTGCCCTAAAAAGCAAAACCATTCTGAGTGACATTGAAAGATGGCAGAAGGGTGTTGCAAGCACTGATGTTTTTGGAGTTCCGATTGAAGTCACTGTGCAACGGCAACAATCTAGCAGGCCTATTCCTCATATTTTGGTGAATTGTGCAGATTATCTCATATTATCAGGGCTAAACACACCATATCTGTTTAAAGGCGAGGGAGATAAAAAAGTTATCCAACAATTGATTTCGATGTATAACCAAGATTCAAATGCATCAATACCAGAGGGTGTAAGTTCAATTGATGTAGCGGTTCTAGTCAAATGTTACCTAGCTACCCTTCCTGAGCCACTAACCACACTTGAGCTTTATAATGAGATCAGAGGTGCTCGGTCTAGCATAAATGCAATGAGAAACATGCTGAAGAGGCTGCCTACTGTAAACTATACAACACTGGAATTTATTACTGCACTACTGCTGCGTGTTAGCCAAAAGTCAGTTCTTAACAAGATGGACACCCAGAGTCTTGCAATGGAGATGGCCCCTGTTATAATTTGGCAGAAGGGTCGGGCACCAGACCTATATAAAAAGTATTGGAATCAACCATCAAAAAGTCTTTCCAAGAAAAACTCGGATCCGGAGCCTACTTATAGTGCATGGGACATGCTTTCTGATGAAGGTGATACTATAGATGATTCCATTCCCTTGGATGATGGAGTGCCTGTTGACTTGGGCGCTATTGAGGTTGTTCAGTGTCTCATGGAACATCACAATGCGATTTTCACAGATGCAAACGAGACAGTCTGGAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0008150 GO:0043087 GO:0044424 GO:0044464 GO:0050790 GO:0051336 GO:0065007 GO:0065009
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RhoGAP

Protein Analysis

455

Amino Acids

50.53

Weight (kDa)

6.07

Isoelectric Point (pI)

42.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RhoGAP PF00620 233 - 372 3.4e-30 RhoGAP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 924, 1103
AasI GACNNNNNNGTC 2 cut(s) 53, 80
Acc36I ACCTGC 1 cut(s) 43
AccB1I GGYRCC 1 cut(s) 1123
AccII CGCG 1 cut(s) 90
AccIII TCCGGA 1 cut(s) 1189
AciI CCGC 5 cut(s) 88, 90, 106, 114, 863
AclWI GGATC 3 cut(s) 196, 1181, 1194
AcoI YGGCCR 2 cut(s) 69, 176
AcsI RAATTY 1 cut(s) 1010
AcuI CTGAAG 2 cut(s) 410, 995
AfaI GTAC 1 cut(s) 453
AfiI CCNNNNNNNGG 1 cut(s) 185
AgsI TTSAA 8 cut(s) 34, 61, 406, 554, 608, 659, 822, 852
AjnI CCWGG 1 cut(s) 267
AjuI GAANNNNNNNTTGG 2 cut(s) 1035, 1067
AluBI AGCT 6 cut(s) 79, 231, 314, 449, 887, 919
AluI AGCT 6 cut(s) 79, 231, 314, 449, 887, 919
Alw21I GWGCWC 2 cut(s) 81, 943
Alw26I GTCTC 4 cut(s) 88, 509, 1313, 1343
AlwI GGATC 3 cut(s) 196, 1181, 1194
Aor13HI TCCGGA 1 cut(s) 1189
AoxI GGCC 4 cut(s) 69, 176, 689, 1092
ApeKI GCWGC 3 cut(s) 430, 982, 1030
ApoI RAATTY 1 cut(s) 1010
AspLEI GCGC 2 cut(s) 462, 1289
AspS9I GGNCC 2 cut(s) 19, 1093
AsuC2I CCSGG 1 cut(s) 175
AsuHPI GGTGA 3 cut(s) 310, 721, 1244
AvaII GGWCC 1 cut(s) 19
AxyI CCTNAGG 1 cut(s) 292
BaeGI GKGCMC 1 cut(s) 1126
BamHI GGATCC 1 cut(s) 1186
BanI GGYRCC 1 cut(s) 1123
BanII GRGCYC 1 cut(s) 81
BbsI GAAGAC 4 cut(s) 49, 135, 176, 575
Bbv12I GWGCWC 2 cut(s) 81, 943
BbvI GCAGC 3 cut(s) 442, 969, 1017
BccI CCATC 7 cut(s) 408, 442, 606, 1057, 1084, 1165, 1259
BceAI ACGGC 3 cut(s) 136, 339, 689
BcgI CGANNNNNNTGC 2 cut(s) 1100, 1134
BciT130I CCWGG 1 cut(s) 269
BclI TGATCA 1 cut(s) 253
BcnI CCSGG 1 cut(s) 175
BcoDI GTCTC 4 cut(s) 88, 509, 1313, 1343
BfaI CTAG 5 cut(s) 441, 684, 869, 884, 948
BfmI CTRYAG 1 cut(s) 1239
BfoI RGCGCY 2 cut(s) 463, 1290
BfuAI ACCTGC 1 cut(s) 43
BglII AGATCT 1 cut(s) 27
BisI GCNGC 4 cut(s) 91, 431, 983, 1031
BlsI GCNGC 4 cut(s) 92, 432, 984, 1032
Bme1390I CCNGG 2 cut(s) 175, 269
Bme18I GGWCC 1 cut(s) 19
BmgT120I GGNCC 2 cut(s) 19, 1093
BmiI GGNNCC 4 cut(s) 1095, 1125, 1188, 1194
BmrFI CCNGG 2 cut(s) 175, 269
BmrI ACTGGG 1 cut(s) 15
BmsI GCATC 3 cut(s) 346, 836, 1330
BmuI ACTGGG 1 cut(s) 15
BpiI GAAGAC 4 cut(s) 49, 135, 176, 575
BplI GAGNNNNNCTC 2 cut(s) 68, 100
Bpu10I CCTNAGC 1 cut(s) 897
BpuEI CTTGAG 2 cut(s) 158, 935
BpuMI CCSGG 1 cut(s) 175
BsaBI GATNNNNATC 1 cut(s) 187
BsaJI CCNNGG 3 cut(s) 88, 164, 1257
BsaWI WCCGGW 1 cut(s) 1189
BsaXI ACNNNNNCTCC 2 cut(s) 1260, 1290
Bsc4I CCNNNNNNNGG 1 cut(s) 185
Bse118I RCCGGY 1 cut(s) 21
Bse1I ACTGG 4 cut(s) 10, 280, 490, 1011
Bse21I CCTNAGG 1 cut(s) 292
Bse3DI GCAATG 2 cut(s) 966, 1089
Bse8I GATNNNNATC 1 cut(s) 187
BseAI TCCGGA 1 cut(s) 1189
BseBI CCWGG 1 cut(s) 269
BseDI CCNNGG 3 cut(s) 88, 164, 1257
BseGI GGATG 2 cut(s) 361, 1267
BseJI GATNNNNATC 1 cut(s) 187
BseLI CCNNNNNNNGG 1 cut(s) 185
BseMI GCAATG 2 cut(s) 966, 1089
BseMII CTCAG 4 cut(s) 195, 306, 587, 888
BseNI ACTGG 4 cut(s) 10, 280, 490, 1011
BseRI GAGGAG 1 cut(s) 356
BseSI GKGCMC 1 cut(s) 1126
BseXI GCAGC 3 cut(s) 442, 969, 1017
BseYI CCCAGC 1 cut(s) 326
BsgI GTGCAG 2 cut(s) 738, 1005
Bsh1236I CGCG 1 cut(s) 90
BshFI GGCC 4 cut(s) 71, 178, 691, 1094
BshNI GGYRCC 1 cut(s) 1123
BsiHKAI GWGCWC 2 cut(s) 81, 943
BsiSI CCGG 3 cut(s) 22, 175, 1190
BslFI GGGAC 4 cut(s) 23, 196, 343, 1226
BslI CCNNNNNNNGG 1 cut(s) 185
BsmAI GTCTC 4 cut(s) 88, 509, 1313, 1343
BsmBI CGTCTC 1 cut(s) 88
BsmFI GGGAC 4 cut(s) 23, 196, 343, 1226
BsnI GGCC 4 cut(s) 71, 178, 691, 1094
Bsp1286I GDGCHC 3 cut(s) 81, 943, 1126
Bsp13I TCCGGA 1 cut(s) 1189
Bsp1407I TGTACA 1 cut(s) 451
Bsp143I GATC 5 cut(s) 27, 188, 253, 930, 1186
BspACI CCGC 5 cut(s) 88, 90, 106, 114, 863
BspANI GGCC 4 cut(s) 71, 178, 691, 1094
BspCNI CTCAG 4 cut(s) 196, 305, 588, 889
BspEI TCCGGA 1 cut(s) 1189
BspFNI CGCG 1 cut(s) 90
BspLI GGNNCC 4 cut(s) 1095, 1125, 1188, 1194
BspMI ACCTGC 1 cut(s) 43
BspPI GGATC 3 cut(s) 196, 1181, 1194
BspT107I GGYRCC 1 cut(s) 1123
BsrDI GCAATG 2 cut(s) 966, 1089
BsrFI RCCGGY 1 cut(s) 21
BsrGI TGTACA 1 cut(s) 451
BsrI ACTGG 4 cut(s) 10, 280, 490, 1011
BssAI RCCGGY 1 cut(s) 21
BssECI CCNNGG 3 cut(s) 88, 164, 1257
BssMI GATC 5 cut(s) 27, 188, 253, 930, 1186
BssT1I CCWWGG 2 cut(s) 164, 1257
Bst2UI CCWGG 1 cut(s) 269
Bst4CI ACNGT 3 cut(s) 667, 991, 1354
Bst6I CTCTTC 2 cut(s) 549, 971
BstAUI TGTACA 1 cut(s) 451
BstC8I GCNNGC 2 cut(s) 631, 689
BstDEI CTNAG 5 cut(s) 204, 292, 351, 596, 897
BstDSI CCRYGG 1 cut(s) 88
BstEII GGTNACC 1 cut(s) 158
BstF5I GGATG 2 cut(s) 361, 1267
BstFNI CGCG 1 cut(s) 90
BstH2I RGCGCY 2 cut(s) 463, 1290
BstHHI GCGC 2 cut(s) 462, 1289
BstKTI GATC 5 cut(s) 30, 191, 256, 933, 1189
BstMAI GTCTC 4 cut(s) 88, 509, 1313, 1343
BstMBI GATC 5 cut(s) 27, 188, 253, 930, 1186
BstMWI GCNNNNNNNGC 1 cut(s) 979
BstNI CCWGG 1 cut(s) 269
BstNSI RCATGY 2 cut(s) 973, 1219
BstPI GGTNACC 1 cut(s) 158
BstSCI CCNGG 2 cut(s) 173, 267
BstSFI CTRYAG 1 cut(s) 1239
BstSLI GKGCMC 1 cut(s) 1126
BstUI CGCG 1 cut(s) 90
BstV1I GCAGC 3 cut(s) 442, 969, 1017
BstV2I GAAGAC 4 cut(s) 49, 135, 176, 575
BstX2I RGATCY 2 cut(s) 27, 1186
BstXI CCANNNNNNTGG 2 cut(s) 275, 1258
BstYI RGATCY 2 cut(s) 27, 1186
Bsu36I CCTNAGG 1 cut(s) 292
BsuRI GGCC 4 cut(s) 71, 178, 691, 1094
BtgI CCRYGG 1 cut(s) 88
BtsCI GGATG 2 cut(s) 361, 1267
BtsIMutI CAGTG 4 cut(s) 633, 663, 1004, 1310
BveI ACCTGC 1 cut(s) 43
Cac8I GCNNGC 2 cut(s) 631, 689
CfoI GCGC 2 cut(s) 462, 1289
Cfr10I RCCGGY 1 cut(s) 21
Cfr13I GGNCC 2 cut(s) 19, 1093
Cfr42I CCGCGG 1 cut(s) 91
CpoI CGGWCCG 1 cut(s) 19
Csp6I GTAC 1 cut(s) 452
CspI CGGWCCG 1 cut(s) 19
CviAII CATG 4 cut(s) 970, 1209, 1216, 1312
CviQI GTAC 1 cut(s) 452
DdeI CTNAG 5 cut(s) 204, 292, 351, 596, 897
DpnI GATC 5 cut(s) 29, 190, 255, 932, 1188
DpnII GATC 5 cut(s) 27, 188, 253, 930, 1186
DraI TTTAAA 1 cut(s) 763
DrdI GACNNNNNNGTC 2 cut(s) 53, 80
DseDI GACNNNNNNGTC 2 cut(s) 53, 80
EaeI YGGCCR 2 cut(s) 69, 176
Eam1104I CTCTTC 2 cut(s) 549, 971
EarI CTCTTC 2 cut(s) 549, 971
Ecl136II GAGCTC 1 cut(s) 79
Eco130I CCWWGG 2 cut(s) 164, 1257
Eco147I AGGCCT 1 cut(s) 691
Eco24I GRGCYC 1 cut(s) 81
Eco32I GATATC 1 cut(s) 42
Eco47I GGWCC 1 cut(s) 19
Eco53kI GAGCTC 1 cut(s) 79
Eco57I CTGAAG 2 cut(s) 410, 995
Eco81I CCTNAGG 1 cut(s) 292
Eco91I GGTNACC 1 cut(s) 158
EcoICRI GAGCTC 1 cut(s) 79
EcoO65I GGTNACC 1 cut(s) 158
EcoRII CCWGG 1 cut(s) 267
EcoRV GATATC 1 cut(s) 42
EcoT14I CCWWGG 2 cut(s) 164, 1257
EcoT22I ATGCAT 1 cut(s) 829
EcoT38I GRGCYC 1 cut(s) 81
ErhI CCWWGG 2 cut(s) 164, 1257
Esp3I CGTCTC 1 cut(s) 88
FaeI CATG 4 cut(s) 973, 1212, 1219, 1315
FaqI GGGAC 4 cut(s) 23, 196, 343, 1226
FatI CATG 4 cut(s) 969, 1208, 1215, 1311
FauI CCCGC 1 cut(s) 95
FbaI TGATCA 1 cut(s) 253
Fnu4HI GCNGC 4 cut(s) 91, 431, 983, 1031
FokI GGATG 2 cut(s) 368, 1274
FriOI GRGCYC 1 cut(s) 81
Fsp4HI GCNGC 4 cut(s) 91, 431, 983, 1031
FspBI CTAG 5 cut(s) 441, 684, 869, 884, 948
GlaI GCGC 2 cut(s) 461, 1288
GluI GCNGC 4 cut(s) 91, 431, 983, 1031
GsaI CCCAGC 1 cut(s) 330
HaeII RGCGCY 2 cut(s) 463, 1290
HaeIII GGCC 4 cut(s) 71, 178, 691, 1094
HapII CCGG 3 cut(s) 22, 175, 1190
HhaI GCGC 2 cut(s) 462, 1289
Hin1II CATG 4 cut(s) 973, 1212, 1219, 1315
Hin6I GCGC 2 cut(s) 460, 1287
HinP1I GCGC 2 cut(s) 460, 1287
HincII GTYRAC 1 cut(s) 1279
HindII GTYRAC 1 cut(s) 1279
HindIII AAGCTT 1 cut(s) 229
HinfI GANTC 9 cut(s) 14, 140, 183, 304, 421, 818, 1075, 1150, 1247
HpaII CCGG 3 cut(s) 22, 175, 1190
HphI GGTGA 3 cut(s) 310, 721, 1244
Hpy166II GTNNAC 2 cut(s) 994, 1279
Hpy188III TCNNGA 8 cut(s) 31, 192, 251, 308, 406, 896, 1190, 1357
Hpy8I GTNNAC 2 cut(s) 994, 1279
Hpy99I CGWCG 4 cut(s) 77, 151, 157, 226
HpyAV CCTTC 6 cut(s) 473, 496, 613, 902, 1108, 1223
HpyCH4III ACNGT 3 cut(s) 667, 991, 1354
HpyCH4V TGCA 9 cut(s) 629, 670, 719, 827, 959, 1022, 1082, 1208, 1343
HpyF10VI GCNNNNNNNGC 1 cut(s) 979
HpyF3I CTNAG 5 cut(s) 204, 292, 351, 596, 897
Hsp92II CATG 4 cut(s) 973, 1212, 1219, 1315
HspAI GCGC 2 cut(s) 460, 1287
Kpn2I TCCGGA 1 cut(s) 1189
Ksp22I TGATCA 1 cut(s) 253
KspI CCGCGG 1 cut(s) 91
Kzo9I GATC 5 cut(s) 27, 188, 253, 930, 1186
LmnI GCTCC 2 cut(s) 457, 1192
Lsp1109I GCAGC 3 cut(s) 442, 969, 1017
LweI GCATC 3 cut(s) 346, 836, 1330
MaeI CTAG 5 cut(s) 441, 684, 869, 884, 948
MaeIII GTNAC 5 cut(s) 158, 346, 599, 661, 878
MalI GATC 5 cut(s) 29, 190, 255, 932, 1188
MboI GATC 5 cut(s) 27, 188, 253, 930, 1186
MfeI CAATTG 2 cut(s) 794, 852
MflI RGATCY 2 cut(s) 27, 1186
MhlI GDGCHC 3 cut(s) 81, 943, 1126
MluCI AATT 5 cut(s) 712, 794, 852, 1010, 1104
MlyI GAGTC 2 cut(s) 149, 1084
MmeI TCCRAC 3 cut(s) 204, 492, 814
Mph1103I ATGCAT 1 cut(s) 829
MroI TCCGGA 1 cut(s) 1189
MseI TTAA 3 cut(s) 366, 762, 1056
MslI CAYNNNNRTG 1 cut(s) 1323
MspA1I CMGCKG 1 cut(s) 90
MspI CCGG 3 cut(s) 22, 175, 1190
MspR9I CCNGG 2 cut(s) 175, 269
MunI CAATTG 2 cut(s) 794, 852
MvaI CCWGG 1 cut(s) 269
MvnI CGCG 1 cut(s) 90
MwoI GCNNNNNNNGC 1 cut(s) 979
NciI CCSGG 1 cut(s) 175
NdeII GATC 5 cut(s) 27, 188, 253, 930, 1186
NlaIII CATG 4 cut(s) 973, 1212, 1219, 1315
NlaIV GGNNCC 4 cut(s) 1095, 1125, 1188, 1194
NmuCI GTSAC 2 cut(s) 599, 661
NsiI ATGCAT 1 cut(s) 829
NspI RCATGY 2 cut(s) 973, 1219
PceI AGGCCT 1 cut(s) 691
PfeI GAWTC 7 cut(s) 14, 183, 304, 421, 818, 1150, 1247
PkrI GCNGC 4 cut(s) 92, 432, 984, 1032
PleI GAGTC 2 cut(s) 148, 1083
PpsI GAGTC 2 cut(s) 148, 1083
PsiI TTATAA 2 cut(s) 924, 1103
Psp124BI GAGCTC 1 cut(s) 81
Psp6I CCWGG 1 cut(s) 267
PspEI GGTNACC 1 cut(s) 158
PspFI CCCAGC 1 cut(s) 326
PspGI CCWGG 1 cut(s) 267
PspN4I GGNNCC 4 cut(s) 1095, 1125, 1188, 1194
PspPI GGNCC 2 cut(s) 19, 1093
PsuI RGATCY 2 cut(s) 27, 1186
RsaI GTAC 1 cut(s) 453
RsaNI GTAC 1 cut(s) 452
RseI CAYNNNNRTG 1 cut(s) 1323
Rsr2I CGGWCCG 1 cut(s) 19
RsrII CGGWCCG 1 cut(s) 19
SacI GAGCTC 1 cut(s) 81
SacII CCGCGG 1 cut(s) 91
SaqAI TTAA 3 cut(s) 366, 762, 1056
SatI GCNGC 4 cut(s) 91, 431, 983, 1031
Sau3AI GATC 5 cut(s) 27, 188, 253, 930, 1186
Sau96I GGNCC 2 cut(s) 19, 1093
SchI GAGTC 2 cut(s) 149, 1084
ScrFI CCNGG 2 cut(s) 175, 269
SduI GDGCHC 3 cut(s) 81, 943, 1126
SfaNI GCATC 3 cut(s) 346, 836, 1330
SfcI CTRYAG 1 cut(s) 1239
Sfr303I CCGCGG 1 cut(s) 91
SgrBI CCGCGG 1 cut(s) 91
SinI GGWCC 1 cut(s) 19
SmiMI CAYNNNNRTG 1 cut(s) 1323
SmlI CTYRAG 2 cut(s) 137, 914
SmoI CTYRAG 2 cut(s) 137, 914
Sse9I AATT 5 cut(s) 712, 794, 852, 1010, 1104
SseBI AGGCCT 1 cut(s) 691
SsiI CCGC 5 cut(s) 88, 90, 106, 114, 863
SspMI CTAG 5 cut(s) 441, 684, 869, 884, 948
SstI GAGCTC 1 cut(s) 81
StuI AGGCCT 1 cut(s) 691
StyD4I CCNGG 2 cut(s) 173, 267
StyI CCWWGG 2 cut(s) 164, 1257
TaaI ACNGT 3 cut(s) 667, 991, 1354
TaqI TCGA 3 cut(s) 38, 191, 803
TaqII GACCGA 1 cut(s) 933
TasI AATT 5 cut(s) 712, 794, 852, 1010, 1104
TatI WGTACW 1 cut(s) 451
TauI GCSGC 1 cut(s) 93
TfiI GAWTC 7 cut(s) 14, 183, 304, 421, 818, 1150, 1247
Tru1I TTAA 3 cut(s) 366, 762, 1056
Tru9I TTAA 3 cut(s) 366, 762, 1056
TscAI CASTG 4 cut(s) 640, 670, 1011, 1310
TseFI GTSAC 2 cut(s) 599, 661
TseI GCWGC 3 cut(s) 430, 982, 1030
Tsp45I GTSAC 2 cut(s) 599, 661
TspDTI ATGAA 2 cut(s) 223, 1242
TspGWI ACGGA 1 cut(s) 210
TspRI CASTG 4 cut(s) 640, 670, 1011, 1310
VpaK11BI GGWCC 1 cut(s) 19
XapI RAATTY 1 cut(s) 1010
XceI RCATGY 2 cut(s) 973, 1219
XspI CTAG 5 cut(s) 441, 684, 869, 884, 948
Zsp2I ATGCAT 1 cut(s) 829
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.