pycom07g22340

Rho GTPase-activating protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0005451
Physical Location & Seq
Reverse (-)
23711795 .. 23714558
2764 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g22340.3

Sequence Viewer

Length: 1134 bp
ATGCCTTCTGACATCTCACCCCGGTGGAAAGAAAAGACTACCGAGTTCTTTTCTTCCTCAGGGGTGAAGATTAAAGAAGCTGGACATTCGGCTGGAACATTTGTTGAAGAGGTCACTAAAGATGCAAAAGTGAATGTTGCTGATGTAGCAGAACGAGTTGGGTCAATGTTCAAAAGTCGATGGGCGCTTCTTCAGCAACCAGCTACAAGACATGCTGTTCAGGAACGTCTTATAGTTGCTGCTGCAACAACTGGTACATTTTTTAGGAAGGGCTTATCAGAGACAAAGGAAAAGGTTTCTGTGGGGAAGATAAAAGTTGAAGAGGTGGCAAAGAAGACTGCCCAAAAGAGCAAAACTATTTTGAGTGACATTGAAAGATGGCAGAAGGGTGTTGCAAGCACTGATGTTTTTGGAGTTCCGATTGAAGTTACCGTGCAACGGCAACAATCTAGTAGGCCCATTCCTCAAATTTTGGTCAGATGTGCAGATTATCTCATATCATCAGGACTCAATACACCATATCTGTTTAAAGGCGAGGGAGATAAAAAAGTCATTCAACAACTAGTTTCACTGTACAACCAAGATTCAAATGCATCATTACCAGAGGGTGTAAGTCCAATTGACGTAGCGGCTCTAGTCAAATGTTACCTAGCTACCCTTCCTGAGCCACTAACCACATTTGAGCTTTATAATAGATCGATCAAAGGTGCTCGTTCCAGCATACGTGCGATGAGAAACATGCTCAGGAGGCTGCCTACAGTCAACTACACAACCCTAGAATTTGTCACTGCATTACTGCTCCGCGTTAGCCAGAAGTCAGTTCTTAACAAGATGAACATCCAAAGTCTCACAATGGAGATGGCCCCTGTTATAATTTGGCAAAAGGGTAGGACACCAGACTTATATAGGAAATATTGGAATCAACCGTCAAAAGGTCCTTCCAGGAAGAACTTGGATCCAGAGCCTACTTATTCTGCGTGGGACATGCTTTCTGATGAAGGTGATGATGTAGATGATTCCATCCCCTTGGATGATGGTGTGCCCATGGACCTCGGCGCTATTGAGGTCGTCCAGTGCCTCATGGAACATCACAATGCAATTTTCACAGATGCTAATGAAACAATCTGGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0008150 GO:0043087 GO:0044424 GO:0044464 GO:0050790 GO:0051336 GO:0065007 GO:0065009
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RhoGAP

Protein Analysis

378

Amino Acids

42.14

Weight (kDa)

8.74

Isoelectric Point (pI)

46.76

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 690, 872
AccII CGCG 1 cut(s) 804
AciI CCGC 2 cut(s) 629, 802
AclWI GGATC 2 cut(s) 950, 963
AcsI RAATTY 2 cut(s) 468, 779
AcuI CTGAAG 1 cut(s) 176
AfaI GTAC 2 cut(s) 256, 575
AfiI CCNNNNNNNGG 2 cut(s) 438, 932
AgsI TTSAA 7 cut(s) 107, 172, 320, 374, 425, 557, 588
AhlI ACTAGT 1 cut(s) 562
AjnI CCWGG 1 cut(s) 941
AleI CACNNNNGTG 1 cut(s) 22
AluBI AGCT 4 cut(s) 80, 203, 653, 685
AluI AGCT 4 cut(s) 80, 203, 653, 685
Alw21I GWGCWC 1 cut(s) 712
Alw26I GTCTC 2 cut(s) 275, 851
AlwI GGATC 2 cut(s) 950, 963
AoxI GGCC 2 cut(s) 455, 861
ApeKI GCWGC 3 cut(s) 239, 242, 751
ApoI RAATTY 2 cut(s) 468, 779
AspLEI GCGC 2 cut(s) 187, 1058
AspS9I GGNCC 4 cut(s) 456, 862, 935, 1048
AsuC2I CCSGG 1 cut(s) 22
AsuHPI GGTGA 3 cut(s) 9, 76, 1013
AvaII GGWCC 2 cut(s) 935, 1048
AxyI CCTNAGG 1 cut(s) 58
BaeGI GKGCMC 1 cut(s) 1044
BamHI GGATCC 1 cut(s) 955
BbsI GAAGAC 1 cut(s) 341
Bbv12I GWGCWC 1 cut(s) 712
BbvI GCAGC 3 cut(s) 226, 229, 738
BccI CCATC 5 cut(s) 174, 372, 853, 1028, 1028
BceAI ACGGC 1 cut(s) 455
BciT130I CCWGG 1 cut(s) 943
BcnI CCSGG 1 cut(s) 22
BcoDI GTCTC 2 cut(s) 275, 851
BcuI ACTAGT 1 cut(s) 562
BfaI CTAG 5 cut(s) 450, 563, 635, 650, 776
BfmI CTRYAG 1 cut(s) 756
BfoI RGCGCY 2 cut(s) 188, 1059
BisI GCNGC 4 cut(s) 240, 243, 630, 752
BlsI GCNGC 4 cut(s) 241, 244, 631, 753
Bme1390I CCNGG 2 cut(s) 22, 943
Bme18I GGWCC 2 cut(s) 935, 1048
BmgT120I GGNCC 4 cut(s) 456, 862, 935, 1048
BmiI GGNNCC 2 cut(s) 864, 957
BmrFI CCNGG 2 cut(s) 22, 943
BmsI GCATC 3 cut(s) 112, 602, 1099
BpiI GAAGAC 1 cut(s) 341
Bpu10I CCTNAGC 2 cut(s) 663, 743
BpuMI CCSGG 1 cut(s) 22
Bsa29I ATCGAT 1 cut(s) 698
BsaAI YACGTR 1 cut(s) 725
BsaBI GATNNNNATC 1 cut(s) 836
BsaJI CCNNGG 4 cut(s) 20, 1026, 1044, 1051
Bsc4I CCNNNNNNNGG 2 cut(s) 438, 932
Bse1I ACTGG 2 cut(s) 256, 1072
Bse21I CCTNAGG 1 cut(s) 58
Bse8I GATNNNNATC 1 cut(s) 836
BseBI CCWGG 1 cut(s) 943
BseCI ATCGAT 1 cut(s) 698
BseDI CCNNGG 4 cut(s) 20, 1026, 1044, 1051
BseGI GGATG 3 cut(s) 837, 1020, 1036
BseJI GATNNNNATC 1 cut(s) 836
BseLI CCNNNNNNNGG 2 cut(s) 438, 932
BseMII CTCAG 3 cut(s) 72, 654, 757
BseNI ACTGG 2 cut(s) 256, 1072
BseSI GKGCMC 1 cut(s) 1044
BseXI GCAGC 3 cut(s) 226, 229, 738
BsgI GTGCAG 1 cut(s) 504
Bsh1236I CGCG 1 cut(s) 804
BshFI GGCC 2 cut(s) 457, 863
BshVI ATCGAT 1 cut(s) 698
BsiHKAI GWGCWC 1 cut(s) 712
BsiSI CCGG 1 cut(s) 22
BslFI GGGAC 1 cut(s) 995
BslI CCNNNNNNNGG 2 cut(s) 438, 932
BsmAI GTCTC 2 cut(s) 275, 851
BsmFI GGGAC 1 cut(s) 995
BsnI GGCC 2 cut(s) 457, 863
Bsp1286I GDGCHC 2 cut(s) 712, 1044
Bsp1407I TGTACA 1 cut(s) 573
Bsp143I GATC 3 cut(s) 695, 699, 955
Bsp19I CCATGG 1 cut(s) 1044
BspACI CCGC 2 cut(s) 629, 802
BspANI GGCC 2 cut(s) 457, 863
BspCNI CTCAG 3 cut(s) 71, 655, 756
BspDI ATCGAT 1 cut(s) 698
BspFNI CGCG 1 cut(s) 804
BspLI GGNNCC 2 cut(s) 864, 957
BspPI GGATC 2 cut(s) 950, 963
BsrGI TGTACA 1 cut(s) 573
BsrI ACTGG 2 cut(s) 256, 1072
BssECI CCNNGG 4 cut(s) 20, 1026, 1044, 1051
BssMI GATC 3 cut(s) 695, 699, 955
BssT1I CCWWGG 2 cut(s) 1026, 1044
Bst2UI CCWGG 1 cut(s) 943
Bst4CI ACNGT 4 cut(s) 433, 573, 760, 927
Bst6I CTCTTC 2 cut(s) 102, 315
BstAUI TGTACA 1 cut(s) 573
BstBAI YACGTR 1 cut(s) 725
BstC8I GCNNGC 1 cut(s) 397
BstDEI CTNAG 3 cut(s) 58, 663, 743
BstDSI CCRYGG 1 cut(s) 1044
BstF5I GGATG 3 cut(s) 837, 1020, 1036
BstFNI CGCG 1 cut(s) 804
BstH2I RGCGCY 2 cut(s) 188, 1059
BstHHI GCGC 2 cut(s) 187, 1058
BstKTI GATC 3 cut(s) 698, 702, 958
BstMAI GTCTC 2 cut(s) 275, 851
BstMBI GATC 3 cut(s) 695, 699, 955
BstMWI GCNNNNNNNGC 3 cut(s) 146, 193, 748
BstNI CCWGG 1 cut(s) 943
BstNSI RCATGY 3 cut(s) 215, 742, 988
BstSCI CCNGG 2 cut(s) 20, 941
BstSFI CTRYAG 1 cut(s) 756
BstSLI GKGCMC 1 cut(s) 1044
BstUI CGCG 1 cut(s) 804
BstV1I GCAGC 3 cut(s) 226, 229, 738
BstV2I GAAGAC 1 cut(s) 341
BstX2I RGATCY 1 cut(s) 955
BstXI CCANNNNNNTGG 1 cut(s) 1027
BstYI RGATCY 1 cut(s) 955
Bsu15I ATCGAT 1 cut(s) 698
Bsu36I CCTNAGG 1 cut(s) 58
BsuRI GGCC 2 cut(s) 457, 863
BsuTUI ATCGAT 1 cut(s) 698
BtgI CCRYGG 1 cut(s) 1044
BtgZI GCGATG 1 cut(s) 743
BtsCI GGATG 3 cut(s) 837, 1020, 1036
BtsI GCAGTG 1 cut(s) 786
BtsIMutI CAGTG 4 cut(s) 399, 569, 786, 1079
Cac8I GCNNGC 1 cut(s) 397
CfoI GCGC 2 cut(s) 187, 1058
Cfr13I GGNCC 4 cut(s) 456, 862, 935, 1048
ClaI ATCGAT 1 cut(s) 698
Csp6I GTAC 2 cut(s) 255, 574
CviAII CATG 5 cut(s) 212, 739, 985, 1045, 1081
CviQI GTAC 2 cut(s) 255, 574
DdeI CTNAG 3 cut(s) 58, 663, 743
DpnI GATC 3 cut(s) 697, 701, 957
DpnII GATC 3 cut(s) 695, 699, 955
DraI TTTAAA 1 cut(s) 529
Eam1104I CTCTTC 2 cut(s) 102, 315
EarI CTCTTC 2 cut(s) 102, 315
Eco130I CCWWGG 2 cut(s) 1026, 1044
Eco47I GGWCC 2 cut(s) 935, 1048
Eco57I CTGAAG 1 cut(s) 176
Eco81I CCTNAGG 1 cut(s) 58
EcoO109I RGGNCCY 1 cut(s) 935
EcoRII CCWGG 1 cut(s) 941
EcoT14I CCWWGG 2 cut(s) 1026, 1044
EcoT22I ATGCAT 1 cut(s) 595
ErhI CCWWGG 2 cut(s) 1026, 1044
FaeI CATG 5 cut(s) 215, 742, 988, 1048, 1084
FaqI GGGAC 1 cut(s) 995
FatI CATG 5 cut(s) 211, 738, 984, 1044, 1080
Fnu4HI GCNGC 4 cut(s) 240, 243, 630, 752
FokI GGATG 3 cut(s) 824, 1007, 1043
Fsp4HI GCNGC 4 cut(s) 240, 243, 630, 752
FspBI CTAG 5 cut(s) 450, 563, 635, 650, 776
GlaI GCGC 2 cut(s) 186, 1057
GluI GCNGC 4 cut(s) 240, 243, 630, 752
HaeII RGCGCY 2 cut(s) 188, 1059
HaeIII GGCC 2 cut(s) 457, 863
HapII CCGG 1 cut(s) 22
HhaI GCGC 2 cut(s) 187, 1058
Hin1II CATG 5 cut(s) 215, 742, 988, 1048, 1084
Hin6I GCGC 2 cut(s) 185, 1056
HinP1I GCGC 2 cut(s) 185, 1056
HincII GTYRAC 1 cut(s) 763
HindII GTYRAC 1 cut(s) 763
HinfI GANTC 4 cut(s) 507, 584, 919, 1016
HpaII CCGG 1 cut(s) 22
HphI GGTGA 3 cut(s) 9, 76, 1013
Hpy166II GTNNAC 1 cut(s) 763
Hpy188I TCNGA 5 cut(s) 10, 280, 420, 479, 994
Hpy188III TCNNGA 6 cut(s) 221, 504, 662, 745, 959, 1126
Hpy8I GTNNAC 1 cut(s) 763
HpyAV CCTTC 6 cut(s) 15, 262, 379, 668, 948, 992
HpyCH4III ACNGT 4 cut(s) 433, 573, 760, 927
HpyCH4IV ACGT 3 cut(s) 226, 624, 724
HpyCH4V TGCA 8 cut(s) 125, 245, 395, 436, 485, 593, 791, 1097
HpyF10VI GCNNNNNNNGC 3 cut(s) 146, 193, 748
HpyF3I CTNAG 3 cut(s) 58, 663, 743
HpySE526I ACGT 3 cut(s) 226, 624, 724
Hsp92II CATG 5 cut(s) 215, 742, 988, 1048, 1084
HspAI GCGC 2 cut(s) 185, 1056
Kzo9I GATC 3 cut(s) 695, 699, 955
LmnI GCTCC 1 cut(s) 804
Lsp1109I GCAGC 3 cut(s) 226, 229, 738
LweI GCATC 3 cut(s) 112, 602, 1099
MaeI CTAG 5 cut(s) 450, 563, 635, 650, 776
MaeII ACGT 3 cut(s) 226, 624, 724
MaeIII GTNAC 5 cut(s) 112, 365, 427, 644, 784
MalI GATC 3 cut(s) 697, 701, 957
MboI GATC 3 cut(s) 695, 699, 955
MboII GAAGA 8 cut(s) 45, 79, 119, 182, 319, 332, 346, 958
MfeI CAATTG 1 cut(s) 618
MflI RGATCY 1 cut(s) 955
MhlI GDGCHC 2 cut(s) 712, 1044
MluCI AATT 5 cut(s) 468, 618, 779, 873, 1098
MlyI GAGTC 1 cut(s) 501
Mph1103I ATGCAT 1 cut(s) 595
MseI TTAA 3 cut(s) 72, 528, 825
MslI CAYNNNNRTG 2 cut(s) 22, 1092
MspI CCGG 1 cut(s) 22
MspR9I CCNGG 2 cut(s) 22, 943
MunI CAATTG 1 cut(s) 618
MvaI CCWGG 1 cut(s) 943
MvnI CGCG 1 cut(s) 804
MwoI GCNNNNNNNGC 3 cut(s) 146, 193, 748
NciI CCSGG 1 cut(s) 22
NcoI CCATGG 1 cut(s) 1044
NdeII GATC 3 cut(s) 695, 699, 955
NlaIII CATG 5 cut(s) 215, 742, 988, 1048, 1084
NlaIV GGNNCC 2 cut(s) 864, 957
NmeAIII GCCGAG 1 cut(s) 1032
NmuCI GTSAC 3 cut(s) 112, 365, 784
NsiI ATGCAT 1 cut(s) 595
NspI RCATGY 3 cut(s) 215, 742, 988
OliI CACNNNNGTG 1 cut(s) 22
PfeI GAWTC 3 cut(s) 584, 919, 1016
PfoI TCCNGGA 1 cut(s) 941
PkrI GCNGC 4 cut(s) 241, 244, 631, 753
PleI GAGTC 1 cut(s) 501
PpsI GAGTC 1 cut(s) 501
Ppu21I YACGTR 1 cut(s) 725
PpuMI RGGWCCY 1 cut(s) 935
PsiI TTATAA 2 cut(s) 690, 872
Psp5II RGGWCCY 1 cut(s) 935
Psp6I CCWGG 1 cut(s) 941
PspGI CCWGG 1 cut(s) 941
PspN4I GGNNCC 2 cut(s) 864, 957
PspPI GGNCC 4 cut(s) 456, 862, 935, 1048
PspPPI RGGWCCY 1 cut(s) 935
PsuI RGATCY 1 cut(s) 955
RsaI GTAC 2 cut(s) 256, 575
RsaNI GTAC 2 cut(s) 255, 574
RseI CAYNNNNRTG 2 cut(s) 22, 1092
SaqAI TTAA 3 cut(s) 72, 528, 825
SatI GCNGC 4 cut(s) 240, 243, 630, 752
Sau3AI GATC 3 cut(s) 695, 699, 955
Sau96I GGNCC 4 cut(s) 456, 862, 935, 1048
SchI GAGTC 1 cut(s) 501
ScrFI CCNGG 2 cut(s) 22, 943
SduI GDGCHC 2 cut(s) 712, 1044
SfaNI GCATC 3 cut(s) 112, 602, 1099
SfcI CTRYAG 1 cut(s) 756
SinI GGWCC 2 cut(s) 935, 1048
SmiMI CAYNNNNRTG 2 cut(s) 22, 1092
SpeI ACTAGT 1 cut(s) 562
Sse9I AATT 5 cut(s) 468, 618, 779, 873, 1098
SsiI CCGC 2 cut(s) 629, 802
SspI AATATT 1 cut(s) 914
SspMI CTAG 5 cut(s) 450, 563, 635, 650, 776
StyD4I CCNGG 2 cut(s) 20, 941
StyI CCWWGG 2 cut(s) 1026, 1044
TaaI ACNGT 4 cut(s) 433, 573, 760, 927
TaiI ACGT 3 cut(s) 229, 627, 727
TaqI TCGA 2 cut(s) 178, 698
TasI AATT 5 cut(s) 468, 618, 779, 873, 1098
TatI WGTACW 1 cut(s) 573
TauI GCSGC 1 cut(s) 632
TfiI GAWTC 3 cut(s) 584, 919, 1016
Tru1I TTAA 3 cut(s) 72, 528, 825
Tru9I TTAA 3 cut(s) 72, 528, 825
TscAI CASTG 4 cut(s) 406, 576, 793, 1079
TseFI GTSAC 3 cut(s) 112, 365, 784
TseI GCWGC 3 cut(s) 239, 242, 751
Tsp45I GTSAC 3 cut(s) 112, 365, 784
TspDTI ATGAA 3 cut(s) 848, 1011, 1131
TspRI CASTG 4 cut(s) 406, 576, 793, 1079
VpaK11BI GGWCC 2 cut(s) 935, 1048
XapI RAATTY 2 cut(s) 468, 779
XceI RCATGY 3 cut(s) 215, 742, 988
XcmI CCANNNNNNNNNTGG 1 cut(s) 949
XspI CTAG 5 cut(s) 450, 563, 635, 650, 776
Zsp2I ATGCAT 1 cut(s) 595
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.