Rroxscaffold_4G00283920

Rho GTPase-activating protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0005451
Physical Location & Seq
Forward (+)
5704865 .. 5709273
4409 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00283920.1

Sequence Viewer

Length: 1158 bp
ATGCCTTCGGCTGTCTCGCCACGGTGGCAAGAGAAGGCTACTGGTTTCTTTTCTACCTCAGGGGTGAAGATTAAGGAAGCTGGACAAACGGCCGGGACATTTGTTGAGGAGGTTACGAAGGATGCTAAAGTTAATGTGTCTGATATGGCAGGACGAGTTGGGTCAATGTTCAAGAGTCGATGGGCGATTCTTCAGCAGCCATCGACTAGACAAGCTGTGCAGGAGCGCCTTATAACTGCTGCTGCTACGACTGGCACATTGTTTAGGAAGGGATTGTCGGAGACAAAGGACAAGGTTTCGGTTGGGAAGATCAAAGTTGAAGAGGTGGCAAAGAAGACTGCCCAAAAAAGCAAAACCATTCTGACTGACATTGAAAGATGGCAGAAGGGTGTTGCAAGCACTGATGTTTTTGGAGTTCCGATTGAAGTTACTGTGCAACGGCAACAATCTAGCAGGCCTATTCCTCATATTTTGGTGAACTGTGCAGATTATCTCATATTATCAGGGCTAAACACACCATATCTGTTTAAAGGTGAGGGAGATAAAAAAGTTATCCAACAATTGATTTCGATGTATAACCAAGATTCAAATGCATCAATACCAGAGGGTGTAAGTTCAATTGATGTAGCGGCTCTAGTCAAATGTTACCTAGCTACCCTTCCTGAGCCACTAACCACACTTGAGCTTTATAACGAGATCAGAGGTGCTCGTTCTAGCATAAATGCAATGAGAAACACGCTGAAGAAGCTTCCTACTGTAAACTATACAACACTGGAATTTGTTACTGCACTACTGCTGCGTGTTAGCCAGAAGTCGGTTCTTAACAAGGCAGGATATACCCCTCTCTCTTTCTCTATGGACACCCAAAGTCTTGCAATGGAGATGGCCCCTGTTATAATTTGGCAGAAGGGTCGGGCACCAGACCTATATAAAAAGTATTGGAGTCAACCATCTAAAGGTCTTTCCAAGAAAAACTCAGATCCAGAGCCTACTTATAGTGCATGGGACATGCTTTCTGATGAAGGTGATACTGTAGATGATTCCATTCCCTTGGATGATGGAGTGCCTATTGACTTGGGCGCTATCGAGGTTGTTCAGTGTCTCATGGAACATCACAATGCGGTTTTCACAGATGCGAACGAAACAGTTTGGAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0008150 GO:0043087 GO:0044424 GO:0044464 GO:0050790 GO:0051336 GO:0065007 GO:0065009
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RhoGAP

Protein Analysis

385

Amino Acids

42.42

Weight (kDa)

8.22

Isoelectric Point (pI)

39.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RhoGAP PF00620 155 - 303 4.8e-26 RhoGAP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 233, 690, 896
AccB1I GGYRCC 1 cut(s) 916
AciI CCGC 2 cut(s) 629, 1121
AclWI GGATC 1 cut(s) 974
AcoI YGGCCR 1 cut(s) 90
AcsI RAATTY 1 cut(s) 776
AcuI CTGAAG 2 cut(s) 176, 761
AfiI CCNNNNNNNGG 2 cut(s) 814, 956
AgsI TTSAA 6 cut(s) 172, 320, 374, 425, 588, 618
AluBI AGCT 5 cut(s) 80, 215, 653, 685, 748
AluI AGCT 5 cut(s) 80, 215, 653, 685, 748
Alw21I GWGCWC 1 cut(s) 709
Alw26I GTCTC 3 cut(s) 19, 275, 1106
AlwI GGATC 1 cut(s) 974
AoxI GGCC 3 cut(s) 90, 455, 885
ApeKI GCWGC 4 cut(s) 196, 239, 242, 796
ApoI RAATTY 1 cut(s) 776
AspLEI GCGC 2 cut(s) 228, 1082
AspS9I GGNCC 1 cut(s) 886
AsuC2I CCSGG 1 cut(s) 94
AsuHPI GGTGA 4 cut(s) 76, 487, 545, 1037
AxyI CCTNAGG 1 cut(s) 58
BaeGI GKGCMC 1 cut(s) 919
BanI GGYRCC 1 cut(s) 916
BbsI GAAGAC 1 cut(s) 341
Bbv12I GWGCWC 1 cut(s) 709
BbvI GCAGC 4 cut(s) 208, 226, 229, 783
BccI CCATC 6 cut(s) 174, 208, 372, 877, 958, 1052
BceAI ACGGC 2 cut(s) 105, 455
BcgI CGANNNNNNTGC 2 cut(s) 893, 927
BcnI CCSGG 1 cut(s) 94
BcoDI GTCTC 3 cut(s) 19, 275, 1106
BfaI CTAG 5 cut(s) 207, 450, 635, 650, 714
BfmI CTRYAG 1 cut(s) 1032
BfoI RGCGCY 2 cut(s) 229, 1083
BglI GCCNNNNNGGC 1 cut(s) 25
BisI GCNGC 5 cut(s) 197, 240, 243, 630, 797
BlsI GCNGC 5 cut(s) 198, 241, 244, 631, 798
Bme1390I CCNGG 1 cut(s) 94
BmgT120I GGNCC 1 cut(s) 886
BmiI GGNNCC 2 cut(s) 888, 918
BmrFI CCNGG 1 cut(s) 94
BmsI GCATC 3 cut(s) 112, 602, 1123
BpiI GAAGAC 1 cut(s) 341
Bpu10I CCTNAGC 1 cut(s) 663
BpuEI CTTGAG 1 cut(s) 701
BpuMI CCSGG 1 cut(s) 94
BsaJI CCNNGG 2 cut(s) 20, 1050
Bsc4I CCNNNNNNNGG 2 cut(s) 814, 956
Bse1I ACTGG 3 cut(s) 46, 256, 777
Bse21I CCTNAGG 1 cut(s) 58
Bse3DI GCAATG 2 cut(s) 732, 882
BseDI CCNNGG 2 cut(s) 20, 1050
BseGI GGATG 2 cut(s) 127, 1060
BseLI CCNNNNNNNGG 2 cut(s) 814, 956
BseMI GCAATG 2 cut(s) 732, 882
BseMII CTCAG 3 cut(s) 72, 654, 990
BseNI ACTGG 3 cut(s) 46, 256, 777
BseRI GAGGAG 1 cut(s) 122
BseSI GKGCMC 1 cut(s) 919
BseX3I CGGCCG 1 cut(s) 90
BseXI GCAGC 4 cut(s) 208, 226, 229, 783
BsgI GTGCAG 3 cut(s) 239, 504, 771
Bsh1285I CGRYCG 1 cut(s) 93
BshFI GGCC 3 cut(s) 92, 457, 887
BshNI GGYRCC 1 cut(s) 916
BsiEI CGRYCG 1 cut(s) 93
BsiHKAI GWGCWC 1 cut(s) 709
BsiSI CCGG 1 cut(s) 93
BslFI GGGAC 2 cut(s) 109, 1019
BslI CCNNNNNNNGG 2 cut(s) 814, 956
BsmAI GTCTC 3 cut(s) 19, 275, 1106
BsmFI GGGAC 2 cut(s) 109, 1019
BsnI GGCC 3 cut(s) 92, 457, 887
Bsp1286I GDGCHC 2 cut(s) 709, 919
Bsp143I GATC 3 cut(s) 309, 696, 979
BspACI CCGC 2 cut(s) 629, 1121
BspANI GGCC 3 cut(s) 92, 457, 887
BspCNI CTCAG 3 cut(s) 71, 655, 989
BspLI GGNNCC 2 cut(s) 888, 918
BspPI GGATC 1 cut(s) 974
BspT107I GGYRCC 1 cut(s) 916
BsrDI GCAATG 2 cut(s) 732, 882
BsrI ACTGG 3 cut(s) 46, 256, 777
BssECI CCNNGG 2 cut(s) 20, 1050
BssMI GATC 3 cut(s) 309, 696, 979
BssT1I CCWWGG 1 cut(s) 1050
Bst4CI ACNGT 6 cut(s) 24, 433, 482, 757, 1033, 1147
Bst6I CTCTTC 1 cut(s) 315
BstC8I GCNNGC 2 cut(s) 397, 455
BstDEI CTNAG 3 cut(s) 58, 663, 976
BstDSI CCRYGG 1 cut(s) 20
BstF5I GGATG 2 cut(s) 127, 1060
BstH2I RGCGCY 2 cut(s) 229, 1083
BstHHI GCGC 2 cut(s) 228, 1082
BstKTI GATC 3 cut(s) 312, 699, 982
BstMAI GTCTC 3 cut(s) 19, 275, 1106
BstMBI GATC 3 cut(s) 309, 696, 979
BstMCI CGRYCG 1 cut(s) 93
BstMWI GCNNNNNNNGC 2 cut(s) 25, 745
BstNSI RCATGY 1 cut(s) 1012
BstSCI CCNGG 1 cut(s) 92
BstSFI CTRYAG 1 cut(s) 1032
BstSLI GKGCMC 1 cut(s) 919
BstV1I GCAGC 4 cut(s) 208, 226, 229, 783
BstV2I GAAGAC 1 cut(s) 341
BstX2I RGATCY 1 cut(s) 979
BstXI CCANNNNNNTGG 1 cut(s) 1051
BstYI RGATCY 1 cut(s) 979
BstZI CGGCCG 1 cut(s) 90
Bsu36I CCTNAGG 1 cut(s) 58
BsuRI GGCC 3 cut(s) 92, 457, 887
BtgI CCRYGG 1 cut(s) 20
BtsCI GGATG 2 cut(s) 127, 1060
BtsIMutI CAGTG 3 cut(s) 399, 770, 1103
Cac8I GCNNGC 2 cut(s) 397, 455
CfoI GCGC 2 cut(s) 228, 1082
Cfr13I GGNCC 1 cut(s) 886
CviAII CATG 3 cut(s) 1002, 1009, 1105
DdeI CTNAG 3 cut(s) 58, 663, 976
DpnI GATC 3 cut(s) 311, 698, 981
DpnII GATC 3 cut(s) 309, 696, 979
DraI TTTAAA 1 cut(s) 529
EaeI YGGCCR 1 cut(s) 90
EagI CGGCCG 1 cut(s) 90
Eam1104I CTCTTC 1 cut(s) 315
EarI CTCTTC 1 cut(s) 315
EclXI CGGCCG 1 cut(s) 90
Eco130I CCWWGG 1 cut(s) 1050
Eco147I AGGCCT 1 cut(s) 457
Eco52I CGGCCG 1 cut(s) 90
Eco57I CTGAAG 2 cut(s) 176, 761
Eco81I CCTNAGG 1 cut(s) 58
EcoT14I CCWWGG 1 cut(s) 1050
EcoT22I ATGCAT 1 cut(s) 595
ErhI CCWWGG 1 cut(s) 1050
FaeI CATG 3 cut(s) 1005, 1012, 1108
FaqI GGGAC 2 cut(s) 109, 1019
FatI CATG 3 cut(s) 1001, 1008, 1104
Fnu4HI GCNGC 5 cut(s) 197, 240, 243, 630, 797
FokI GGATG 2 cut(s) 134, 1067
Fsp4HI GCNGC 5 cut(s) 197, 240, 243, 630, 797
FspBI CTAG 5 cut(s) 207, 450, 635, 650, 714
GlaI GCGC 2 cut(s) 227, 1081
GluI GCNGC 5 cut(s) 197, 240, 243, 630, 797
HaeII RGCGCY 2 cut(s) 229, 1083
HaeIII GGCC 3 cut(s) 92, 457, 887
HapII CCGG 1 cut(s) 93
HhaI GCGC 2 cut(s) 228, 1082
Hin1II CATG 3 cut(s) 1005, 1012, 1108
Hin6I GCGC 2 cut(s) 226, 1080
HinP1I GCGC 2 cut(s) 226, 1080
HincII GTYRAC 1 cut(s) 947
HindII GTYRAC 1 cut(s) 947
HindIII AAGCTT 1 cut(s) 746
HinfI GANTC 5 cut(s) 175, 187, 584, 943, 1040
HpaII CCGG 1 cut(s) 93
HphI GGTGA 4 cut(s) 76, 487, 545, 1037
Hpy166II GTNNAC 3 cut(s) 478, 760, 947
Hpy188I TCNGA 7 cut(s) 142, 280, 363, 420, 701, 979, 1018
Hpy188III TCNNGA 3 cut(s) 172, 662, 983
Hpy8I GTNNAC 3 cut(s) 478, 760, 947
HpyAV CCTTC 8 cut(s) 15, 28, 112, 262, 379, 668, 901, 1016
HpyCH4III ACNGT 6 cut(s) 24, 433, 482, 757, 1033, 1147
HpyCH4V TGCA 9 cut(s) 220, 395, 436, 485, 593, 725, 788, 875, 1001
HpyF10VI GCNNNNNNNGC 2 cut(s) 25, 745
HpyF3I CTNAG 3 cut(s) 58, 663, 976
Hsp92II CATG 3 cut(s) 1005, 1012, 1108
HspAI GCGC 2 cut(s) 226, 1080
Kzo9I GATC 3 cut(s) 309, 696, 979
LmnI GCTCC 1 cut(s) 223
Lsp1109I GCAGC 4 cut(s) 208, 226, 229, 783
LweI GCATC 3 cut(s) 112, 602, 1123
MaeI CTAG 5 cut(s) 207, 450, 635, 650, 714
MaeIII GTNAC 4 cut(s) 112, 427, 644, 781
MalI GATC 3 cut(s) 311, 698, 981
MboI GATC 3 cut(s) 309, 696, 979
MboII GAAGA 6 cut(s) 79, 182, 319, 332, 346, 754
MfeI CAATTG 2 cut(s) 560, 618
MflI RGATCY 1 cut(s) 979
MhlI GDGCHC 2 cut(s) 709, 919
MluCI AATT 4 cut(s) 560, 618, 776, 897
MlyI GAGTC 2 cut(s) 184, 952
MmeI TCCRAC 2 cut(s) 258, 580
Mph1103I ATGCAT 1 cut(s) 595
MseI TTAA 4 cut(s) 72, 132, 528, 822
MslI CAYNNNNRTG 1 cut(s) 1116
MspI CCGG 1 cut(s) 93
MspR9I CCNGG 1 cut(s) 94
MunI CAATTG 2 cut(s) 560, 618
MwoI GCNNNNNNNGC 2 cut(s) 25, 745
NciI CCSGG 1 cut(s) 94
NdeII GATC 3 cut(s) 309, 696, 979
NlaIII CATG 3 cut(s) 1005, 1012, 1108
NlaIV GGNNCC 2 cut(s) 888, 918
NsiI ATGCAT 1 cut(s) 595
NspI RCATGY 1 cut(s) 1012
PceI AGGCCT 1 cut(s) 457
PfeI GAWTC 3 cut(s) 187, 584, 1040
PkrI GCNGC 5 cut(s) 198, 241, 244, 631, 798
PleI GAGTC 2 cut(s) 183, 951
PpsI GAGTC 2 cut(s) 183, 951
PsiI TTATAA 3 cut(s) 233, 690, 896
PspN4I GGNNCC 2 cut(s) 888, 918
PspPI GGNCC 1 cut(s) 886
PsuI RGATCY 1 cut(s) 979
RseI CAYNNNNRTG 1 cut(s) 1116
SaqAI TTAA 4 cut(s) 72, 132, 528, 822
SatI GCNGC 5 cut(s) 197, 240, 243, 630, 797
Sau3AI GATC 3 cut(s) 309, 696, 979
Sau96I GGNCC 1 cut(s) 886
SchI GAGTC 2 cut(s) 184, 952
ScrFI CCNGG 1 cut(s) 94
SduI GDGCHC 2 cut(s) 709, 919
SfaNI GCATC 3 cut(s) 112, 602, 1123
SfcI CTRYAG 1 cut(s) 1032
SmiMI CAYNNNNRTG 1 cut(s) 1116
SmlI CTYRAG 1 cut(s) 680
SmoI CTYRAG 1 cut(s) 680
Sse9I AATT 4 cut(s) 560, 618, 776, 897
SseBI AGGCCT 1 cut(s) 457
SsiI CCGC 2 cut(s) 629, 1121
SspMI CTAG 5 cut(s) 207, 450, 635, 650, 714
StuI AGGCCT 1 cut(s) 457
StyD4I CCNGG 1 cut(s) 92
StyI CCWWGG 1 cut(s) 1050
TaaI ACNGT 6 cut(s) 24, 433, 482, 757, 1033, 1147
TaqI TCGA 4 cut(s) 178, 203, 569, 1086
TasI AATT 4 cut(s) 560, 618, 776, 897
TauI GCSGC 1 cut(s) 632
TfiI GAWTC 3 cut(s) 187, 584, 1040
Tru1I TTAA 4 cut(s) 72, 132, 528, 822
Tru9I TTAA 4 cut(s) 72, 132, 528, 822
TscAI CASTG 3 cut(s) 406, 777, 1103
TseI GCWGC 4 cut(s) 196, 239, 242, 796
TspDTI ATGAA 1 cut(s) 1035
TspRI CASTG 3 cut(s) 406, 777, 1103
XapI RAATTY 1 cut(s) 776
XceI RCATGY 1 cut(s) 1012
XspI CTAG 5 cut(s) 207, 450, 635, 650, 714
Zsp2I ATGCAT 1 cut(s) 595
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.