MD07G1250400.v1.1

Rho GTPase-activating protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0005451
Physical Location & Seq
Reverse (-)
31906183 .. 31910278
4096 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1250400.v1.1.491

Sequence Viewer

Length: 1131 bp
ATGCCTTCCGACATCTCACCCCGGTGGAAAGAAAAGACTACCGAGTTCTTTTCTTCCTCAGGGGTGAAGATTAAAGAAGCTGGACATTCGGCTGGAACATTTGTTGAAGAGGTCACTAAAGATGCAAAAGTGAATGTTGCTGATGTGGCAGAACGAGTTGGGTCAATGTTCAAAAGTCGATGGGCGCTTCTTCAGCAACCAGCTACAAGACATGCTGTTCAGGAACGTCTTATAGTTGCTGCTGCGACAACTGGTACATTTTTTAGGAAGGGCTTATCAGAGACAAAGGAAAAGGTTTCTGTTGGGAAGATAAAAGTTGAAGAGGTGGCAAAGAAGACTGCCCAAAAGAGCAAAACTATTTTGAGTGACATTGAAAGATGGCAGAAGGGTGTTGCAAGCACTGATGTTTTTGGAGTTCCGATTGAAGTTACTGTGCAACGGCAACAATCTAGTAGGCCCATTCCTCAAATTTTGGTCAGATGTGCAGATTATCTCATATCATCAGGACTAAATACACCATATCTGTTTAAAGACGAGGGAGATAAAAAAGTCATTCAACAACTGGTTTCACTGTACAACCAAGATTCAAATGCATCATTACCAGAGGGTGTAAGTCCAATTGACGTAGCGGCTTTAGTCAAATGTTACCTAGCTACCCTTCCTGAGCCACTGACCACATTTGAGCTTTATAATGAGATCATAGGTGCTCGTTCCAGCATACGTGAGATGAGAAACATGCTCAGGAGGCTTCCTACAGTCAACTACACAACCCTAGAATTTGTCACTGCATTACTGCTCCGCATTAGCCAGAAGTCAGTTCTTAACAAGATGAACATCCAAAGTCTTGCAATGGAGATGGCCCCTGTTATAATTTGGCAGAAGGGTAGGACACCAGACCTATACAGGAAATATTGGAATCAACCGTCAAAAGGTCCTTCCAGGAAGAACTTGGATCCAGAGCCTACTTATTCTGCGTGGGACATGCTTTCTGATGAAGGCGATGCTGTAGATGATTCAATCCCCTTGGATGATGGTGTGCCCATGGACCTCGGCGCTATTGAGGTCGTCCAGTGCCTCATGGAACATCACAATGCAATTTTCACAGATGCTAATGAAACAATCTGGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0008150 GO:0043087 GO:0044424 GO:0044464 GO:0050790 GO:0051336 GO:0065007 GO:0065009
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RhoGAP

Protein Analysis

377

Amino Acids

42.07

Weight (kDa)

6.93

Isoelectric Point (pI)

47.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RhoGAP PF00620 155 - 294 1.8e-32 RhoGAP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 690, 869
AciI CCGC 2 cut(s) 629, 799
AclWI GGATC 2 cut(s) 947, 960
AcsI RAATTY 2 cut(s) 468, 776
AcuI CTGAAG 1 cut(s) 176
AfaI GTAC 2 cut(s) 256, 575
AfiI CCNNNNNNNGG 1 cut(s) 929
AgsI TTSAA 8 cut(s) 107, 172, 320, 374, 425, 557, 588, 1017
AjnI CCWGG 1 cut(s) 938
AleI CACNNNNGTG 1 cut(s) 22
AluBI AGCT 4 cut(s) 80, 203, 653, 685
AluI AGCT 4 cut(s) 80, 203, 653, 685
Alw21I GWGCWC 1 cut(s) 709
Alw26I GTCTC 1 cut(s) 275
AlwI GGATC 2 cut(s) 947, 960
AoxI GGCC 2 cut(s) 455, 858
ApeKI GCWGC 2 cut(s) 239, 242
ApoI RAATTY 2 cut(s) 468, 776
AspLEI GCGC 2 cut(s) 187, 1055
AspS9I GGNCC 4 cut(s) 456, 859, 932, 1045
AsuC2I CCSGG 1 cut(s) 22
AsuHPI GGTGA 2 cut(s) 9, 76
AvaII GGWCC 2 cut(s) 932, 1045
AxyI CCTNAGG 1 cut(s) 58
BaeGI GKGCMC 1 cut(s) 1041
BamHI GGATCC 1 cut(s) 952
BbsI GAAGAC 1 cut(s) 341
Bbv12I GWGCWC 1 cut(s) 709
BbvI GCAGC 2 cut(s) 226, 229
BccI CCATC 4 cut(s) 174, 372, 850, 1025
BceAI ACGGC 1 cut(s) 455
BciT130I CCWGG 1 cut(s) 940
BcnI CCSGG 1 cut(s) 22
BcoDI GTCTC 1 cut(s) 275
BfaI CTAG 3 cut(s) 450, 650, 773
BfmI CTRYAG 2 cut(s) 753, 1005
BfoI RGCGCY 2 cut(s) 188, 1056
BisI GCNGC 3 cut(s) 240, 243, 630
BlsI GCNGC 3 cut(s) 241, 244, 631
Bme1390I CCNGG 2 cut(s) 22, 940
Bme18I GGWCC 2 cut(s) 932, 1045
BmgT120I GGNCC 4 cut(s) 456, 859, 932, 1045
BmiI GGNNCC 2 cut(s) 861, 954
BmrFI CCNGG 2 cut(s) 22, 940
BmsI GCATC 4 cut(s) 112, 602, 991, 1096
BpiI GAAGAC 1 cut(s) 341
Bpu10I CCTNAGC 2 cut(s) 663, 740
BpuMI CCSGG 1 cut(s) 22
BsaAI YACGTR 1 cut(s) 722
BsaBI GATNNNNATC 1 cut(s) 833
BsaJI CCNNGG 4 cut(s) 20, 1023, 1041, 1048
Bsc4I CCNNNNNNNGG 1 cut(s) 929
Bse1I ACTGG 3 cut(s) 256, 567, 1069
Bse21I CCTNAGG 1 cut(s) 58
Bse3DI GCAATG 1 cut(s) 855
Bse8I GATNNNNATC 1 cut(s) 833
BseBI CCWGG 1 cut(s) 940
BseDI CCNNGG 4 cut(s) 20, 1023, 1041, 1048
BseGI GGATG 2 cut(s) 834, 1033
BseJI GATNNNNATC 1 cut(s) 833
BseLI CCNNNNNNNGG 1 cut(s) 929
BseMI GCAATG 1 cut(s) 855
BseMII CTCAG 3 cut(s) 72, 654, 754
BseNI ACTGG 3 cut(s) 256, 567, 1069
BseSI GKGCMC 1 cut(s) 1041
BseXI GCAGC 2 cut(s) 226, 229
BsgI GTGCAG 1 cut(s) 504
BshFI GGCC 2 cut(s) 457, 860
BsiHKAI GWGCWC 1 cut(s) 709
BsiSI CCGG 1 cut(s) 22
BslFI GGGAC 1 cut(s) 992
BslI CCNNNNNNNGG 1 cut(s) 929
BsmAI GTCTC 1 cut(s) 275
BsmFI GGGAC 1 cut(s) 992
BsnI GGCC 2 cut(s) 457, 860
Bsp1286I GDGCHC 2 cut(s) 709, 1041
Bsp1407I TGTACA 1 cut(s) 573
Bsp143I GATC 2 cut(s) 696, 952
Bsp19I CCATGG 1 cut(s) 1041
BspACI CCGC 2 cut(s) 629, 799
BspANI GGCC 2 cut(s) 457, 860
BspCNI CTCAG 3 cut(s) 71, 655, 753
BspLI GGNNCC 2 cut(s) 861, 954
BspPI GGATC 2 cut(s) 947, 960
BsrDI GCAATG 1 cut(s) 855
BsrGI TGTACA 1 cut(s) 573
BsrI ACTGG 3 cut(s) 256, 567, 1069
BssECI CCNNGG 4 cut(s) 20, 1023, 1041, 1048
BssMI GATC 2 cut(s) 696, 952
BssT1I CCWWGG 2 cut(s) 1023, 1041
Bst2UI CCWGG 1 cut(s) 940
Bst4CI ACNGT 4 cut(s) 433, 573, 757, 924
Bst6I CTCTTC 2 cut(s) 102, 315
BstAUI TGTACA 1 cut(s) 573
BstBAI YACGTR 1 cut(s) 722
BstC8I GCNNGC 1 cut(s) 397
BstDEI CTNAG 3 cut(s) 58, 663, 740
BstDSI CCRYGG 1 cut(s) 1041
BstF5I GGATG 2 cut(s) 834, 1033
BstH2I RGCGCY 2 cut(s) 188, 1056
BstHHI GCGC 2 cut(s) 187, 1055
BstKTI GATC 2 cut(s) 699, 955
BstMAI GTCTC 1 cut(s) 275
BstMBI GATC 2 cut(s) 696, 952
BstMWI GCNNNNNNNGC 3 cut(s) 146, 193, 745
BstNI CCWGG 1 cut(s) 940
BstNSI RCATGY 3 cut(s) 215, 739, 985
BstSCI CCNGG 2 cut(s) 20, 938
BstSFI CTRYAG 2 cut(s) 753, 1005
BstSLI GKGCMC 1 cut(s) 1041
BstV1I GCAGC 2 cut(s) 226, 229
BstV2I GAAGAC 1 cut(s) 341
BstX2I RGATCY 1 cut(s) 952
BstYI RGATCY 1 cut(s) 952
Bsu36I CCTNAGG 1 cut(s) 58
BsuRI GGCC 2 cut(s) 457, 860
BtgI CCRYGG 1 cut(s) 1041
BtgZI GCGATG 1 cut(s) 1014
BtsCI GGATG 2 cut(s) 834, 1033
BtsI GCAGTG 1 cut(s) 783
BtsIMutI CAGTG 5 cut(s) 399, 569, 668, 783, 1076
Cac8I GCNNGC 1 cut(s) 397
CfoI GCGC 2 cut(s) 187, 1055
Cfr13I GGNCC 4 cut(s) 456, 859, 932, 1045
Csp6I GTAC 2 cut(s) 255, 574
CviAII CATG 5 cut(s) 212, 736, 982, 1042, 1078
CviQI GTAC 2 cut(s) 255, 574
DdeI CTNAG 3 cut(s) 58, 663, 740
DpnI GATC 2 cut(s) 698, 954
DpnII GATC 2 cut(s) 696, 952
DraI TTTAAA 1 cut(s) 529
Eam1104I CTCTTC 2 cut(s) 102, 315
EarI CTCTTC 2 cut(s) 102, 315
Eco130I CCWWGG 2 cut(s) 1023, 1041
Eco47I GGWCC 2 cut(s) 932, 1045
Eco57I CTGAAG 1 cut(s) 176
Eco81I CCTNAGG 1 cut(s) 58
EcoO109I RGGNCCY 1 cut(s) 932
EcoRII CCWGG 1 cut(s) 938
EcoT14I CCWWGG 2 cut(s) 1023, 1041
EcoT22I ATGCAT 1 cut(s) 595
ErhI CCWWGG 2 cut(s) 1023, 1041
FaeI CATG 5 cut(s) 215, 739, 985, 1045, 1081
FaqI GGGAC 1 cut(s) 992
FatI CATG 5 cut(s) 211, 735, 981, 1041, 1077
Fnu4HI GCNGC 3 cut(s) 240, 243, 630
FokI GGATG 2 cut(s) 821, 1040
Fsp4HI GCNGC 3 cut(s) 240, 243, 630
FspBI CTAG 3 cut(s) 450, 650, 773
GlaI GCGC 2 cut(s) 186, 1054
GluI GCNGC 3 cut(s) 240, 243, 630
HaeII RGCGCY 2 cut(s) 188, 1056
HaeIII GGCC 2 cut(s) 457, 860
HapII CCGG 1 cut(s) 22
HhaI GCGC 2 cut(s) 187, 1055
Hin1II CATG 5 cut(s) 215, 739, 985, 1045, 1081
Hin6I GCGC 2 cut(s) 185, 1053
HinP1I GCGC 2 cut(s) 185, 1053
HincII GTYRAC 1 cut(s) 760
HindII GTYRAC 1 cut(s) 760
HinfI GANTC 3 cut(s) 584, 916, 1013
HpaII CCGG 1 cut(s) 22
HphI GGTGA 2 cut(s) 9, 76
Hpy166II GTNNAC 1 cut(s) 760
Hpy188I TCNGA 5 cut(s) 10, 280, 420, 479, 991
Hpy188III TCNNGA 6 cut(s) 221, 504, 662, 742, 956, 1123
Hpy8I GTNNAC 1 cut(s) 760
HpyAV CCTTC 7 cut(s) 15, 262, 379, 668, 874, 945, 989
HpyCH4III ACNGT 4 cut(s) 433, 573, 757, 924
HpyCH4IV ACGT 3 cut(s) 226, 624, 721
HpyCH4V TGCA 8 cut(s) 125, 395, 436, 485, 593, 788, 848, 1094
HpyF10VI GCNNNNNNNGC 3 cut(s) 146, 193, 745
HpyF3I CTNAG 3 cut(s) 58, 663, 740
HpySE526I ACGT 3 cut(s) 226, 624, 721
Hsp92II CATG 5 cut(s) 215, 739, 985, 1045, 1081
HspAI GCGC 2 cut(s) 185, 1053
Kzo9I GATC 2 cut(s) 696, 952
LmnI GCTCC 1 cut(s) 801
Lsp1109I GCAGC 2 cut(s) 226, 229
LweI GCATC 4 cut(s) 112, 602, 991, 1096
MaeI CTAG 3 cut(s) 450, 650, 773
MaeII ACGT 3 cut(s) 226, 624, 721
MaeIII GTNAC 5 cut(s) 112, 365, 427, 644, 781
MalI GATC 2 cut(s) 698, 954
MboI GATC 2 cut(s) 696, 952
MboII GAAGA 8 cut(s) 45, 79, 119, 182, 319, 332, 346, 955
MfeI CAATTG 1 cut(s) 618
MflI RGATCY 1 cut(s) 952
MhlI GDGCHC 2 cut(s) 709, 1041
MluCI AATT 5 cut(s) 468, 618, 776, 870, 1095
MmeI TCCRAC 1 cut(s) 33
Mph1103I ATGCAT 1 cut(s) 595
MseI TTAA 3 cut(s) 72, 528, 822
MslI CAYNNNNRTG 2 cut(s) 22, 1089
MspI CCGG 1 cut(s) 22
MspR9I CCNGG 2 cut(s) 22, 940
MunI CAATTG 1 cut(s) 618
MvaI CCWGG 1 cut(s) 940
MwoI GCNNNNNNNGC 3 cut(s) 146, 193, 745
NciI CCSGG 1 cut(s) 22
NcoI CCATGG 1 cut(s) 1041
NdeII GATC 2 cut(s) 696, 952
NlaIII CATG 5 cut(s) 215, 739, 985, 1045, 1081
NlaIV GGNNCC 2 cut(s) 861, 954
NmeAIII GCCGAG 1 cut(s) 1029
NmuCI GTSAC 3 cut(s) 112, 365, 781
NsiI ATGCAT 1 cut(s) 595
NspI RCATGY 3 cut(s) 215, 739, 985
OliI CACNNNNGTG 1 cut(s) 22
PfeI GAWTC 3 cut(s) 584, 916, 1013
PfoI TCCNGGA 1 cut(s) 938
PkrI GCNGC 3 cut(s) 241, 244, 631
Ppu21I YACGTR 1 cut(s) 722
PpuMI RGGWCCY 1 cut(s) 932
PsiI TTATAA 2 cut(s) 690, 869
Psp5II RGGWCCY 1 cut(s) 932
Psp6I CCWGG 1 cut(s) 938
PspGI CCWGG 1 cut(s) 938
PspN4I GGNNCC 2 cut(s) 861, 954
PspPI GGNCC 4 cut(s) 456, 859, 932, 1045
PspPPI RGGWCCY 1 cut(s) 932
PsuI RGATCY 1 cut(s) 952
RsaI GTAC 2 cut(s) 256, 575
RsaNI GTAC 2 cut(s) 255, 574
RseI CAYNNNNRTG 2 cut(s) 22, 1089
SaqAI TTAA 3 cut(s) 72, 528, 822
SatI GCNGC 3 cut(s) 240, 243, 630
Sau3AI GATC 2 cut(s) 696, 952
Sau96I GGNCC 4 cut(s) 456, 859, 932, 1045
ScrFI CCNGG 2 cut(s) 22, 940
SduI GDGCHC 2 cut(s) 709, 1041
SfaNI GCATC 4 cut(s) 112, 602, 991, 1096
SfcI CTRYAG 2 cut(s) 753, 1005
SinI GGWCC 2 cut(s) 932, 1045
SmiMI CAYNNNNRTG 2 cut(s) 22, 1089
Sse9I AATT 5 cut(s) 468, 618, 776, 870, 1095
SsiI CCGC 2 cut(s) 629, 799
SspI AATATT 1 cut(s) 911
SspMI CTAG 3 cut(s) 450, 650, 773
StyD4I CCNGG 2 cut(s) 20, 938
StyI CCWWGG 2 cut(s) 1023, 1041
TaaI ACNGT 4 cut(s) 433, 573, 757, 924
TaiI ACGT 3 cut(s) 229, 627, 724
TaqI TCGA 1 cut(s) 178
TasI AATT 5 cut(s) 468, 618, 776, 870, 1095
TatI WGTACW 1 cut(s) 573
TauI GCSGC 1 cut(s) 632
TfiI GAWTC 3 cut(s) 584, 916, 1013
Tru1I TTAA 3 cut(s) 72, 528, 822
Tru9I TTAA 3 cut(s) 72, 528, 822
TscAI CASTG 5 cut(s) 406, 576, 675, 790, 1076
TseFI GTSAC 3 cut(s) 112, 365, 781
TseI GCWGC 2 cut(s) 239, 242
Tsp45I GTSAC 3 cut(s) 112, 365, 781
TspDTI ATGAA 3 cut(s) 845, 1008, 1128
TspRI CASTG 5 cut(s) 406, 576, 675, 790, 1076
VpaK11BI GGWCC 2 cut(s) 932, 1045
XapI RAATTY 2 cut(s) 468, 776
XceI RCATGY 3 cut(s) 215, 739, 985
XcmI CCANNNNNNNNNTGG 1 cut(s) 946
XspI CTAG 3 cut(s) 450, 650, 773
Zsp2I ATGCAT 1 cut(s) 595
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.