AT5G61580

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0008946
Physical Location & Seq
Forward (+)
24761041 .. 24764168
3128 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G61580.1

Sequence Viewer

Length: 1593 bp
ATGGAAGCTTCGATTTCGTTTCTGGGGTCAACAAAACCCAATATTTCCTTGTTTAACCCTTCTTCAAACGTCCTTCCTCGTAGAGATTTCCCTCTTCCTGCTTTGAAATTGAAGAAAGTTTCAGTGCTGCCTCGAATCTTGCACCAGAAACGACTCATCAGAGCTCAGTGCTCTGATGGATTCAAACCAGAGGAAGACGATGGGTTTGTCCTAGAAGACGTTCCTCACTTGACCAAATTTCTCCCTGATTTACCGTCATATCCAAATCCATTGAAAGAAAGCCAAGCATATGCCATTGTTAAGCGAACTTTTGTCAGTTCCGAAGATGTGGTTGCGCAAAATATTGTAGTCCAGAAGGGAAGTAAGCGAGGAGTACACTTTAGGCGAGCAGGGCCTCGAGAAAGAGTGTACTTCAGATCAGATGAAGTAAAAGCTTGCATAGTGACTTGTGGGGGCTTGTGCCCTGGAATCAATACTGTTATACGGGAAATTGTATGTGGATTGAACAATATGTATGGTGTTAATAACATTCTCGGCATTCAGGGAGGATATAGAGGCTTTTACTCCAAAAACACTATGAACCTGACACCTAAAGTAGTTAACGATATTCATAAACGCGGTGGCACTTTTCTTCAAACCTCAAGAGGAGGACATGATACAGCGAAGATTGTTGATAATATTCAAGATAGAGGAATAAATCAGGTATATATTATTGGAGGTGGTGGGACGCAAAAGGGTGCAGAGAAGATATACGAGGAAGTTGAGAGGCGTGGTCTTCAAGTGGCGGTTTCTGGCATTCCTAAGACAATTGATAATGATATTGCTGTGATTGACAAATCATTTGGCTTTGATACGGCGGTTGAGGAAGCACAACGAGCTATTAATGCTGCACATGTAGAGGTCGAGAGCGTGGAAAATGGAGTTGGTATCGTTAAACTCATGGGCAGATACAGTGGTTTTATTGCCATGATTGCAACTTTAGCGAATCGTGATGTGGATTGTTGCTTGATTCCAGAGTCTCCATTTTTTCTTGAAGGAAAGGGTGGGCTCTTTGAGTTTATTGAAGAACGACTCAAAGAGAATAGGCACATGGTTATTGTGATAGCTGAAGGAGCTGGACAGGATTATGTTGCTCAAAGCATGCGTGCATCTGAAACTAAAGACGCCTCAGGAAATAGACTCTTGCTTGATGTTGGTCTATGGTTGACTCAACAGATAAAGGATCACTTTACAAATGTTCGGAAAATGATGATAAATATGAAGTACATAGACCCAACGTATATGATAAGAGCAATACCGAGTAACGCATCAGACAATGTCTATTGCACTCTTCTTGCCCAAAGTGCAGTTCATGGAGCAATGGCTGGGTACTCAGGTTTCACTGTAGGACCAGTTAACAGTAGACATGCTTACATCCCAATTTCTCAGGTGACGGAAGTGACAAATACGGTGAAGTTAACTGATAGGATGTGGGCTAGACTCCTTGCATCGACAAATCAACCGAGTTTCTTGACTGGTGAAGGAGCATTGCAGAATGTGATCGACATGGAAACTCAAGAAAAGATCGATAACATGAAGATCTCTTCTATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003872 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008443 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009507 GO:0009536 GO:0009987 GO:0016052 GO:0016053 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019200 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044262 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046835 GO:0046939 GO:0051186 GO:0051188 GO:0055086 GO:0061615 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00030 ko00051 ko00052 ko00680 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko03018 ko04152 ko05230 map00010 map00030 map00051 map00052 map00680 map01100 map01110 map01120 map01130 map01200 map01230 map03018 map04152 map05230
Pfam Domains
Protein Families
PFK

Protein Analysis

530

Amino Acids

58.47

Weight (kDa)

8.46

Isoelectric Point (pI)

34.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PFK PF00365 145 - 451 2.8e-60 Phosphofructokinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 336
AccI GTMKAC 1 cut(s) 1402
AccII CGCG 1 cut(s) 618
AciI CCGC 3 cut(s) 618, 785, 857
AclWI GGATC 1 cut(s) 1230
AcsI RAATTY 1 cut(s) 236
AcuI CTGAAG 2 cut(s) 397, 1128
AcyI GRCGYC 1 cut(s) 1164
AfaI GTAC 4 cut(s) 375, 410, 1265, 1370
AflIII ACRYGT 1 cut(s) 892
AjnI CCWGG 1 cut(s) 463
AjuI GAANNNNNNNTTGG 4 cut(s) 906, 938, 1332, 1364
AluBI AGCT 6 cut(s) 8, 164, 434, 878, 1106, 1115
AluI AGCT 6 cut(s) 8, 164, 434, 878, 1106, 1115
Alw21I GWGCWC 2 cut(s) 166, 173
Alw26I GTCTC 1 cut(s) 1023
AlwI GGATC 1 cut(s) 1230
Ama87I CYCGRG 1 cut(s) 396
AoxI GGCC 1 cut(s) 392
ApeKI GCWGC 2 cut(s) 127, 887
ApoI RAATTY 1 cut(s) 236
ArsI GACNNNNNNTTYG 4 cut(s) 188, 220, 824, 856
AseI ATTAAT 1 cut(s) 882
Asp700I GAANNNNTTC 1 cut(s) 219
AspLEI GCGC 1 cut(s) 337
AspS9I GGNCC 2 cut(s) 392, 1388
AsuHPI GGTGA 3 cut(s) 1441, 1462, 1529
AvaI CYCGRG 1 cut(s) 396
AvaII GGWCC 1 cut(s) 1388
AxyI CCTNAGG 1 cut(s) 1168
BaeGI GKGCMC 1 cut(s) 464
BaeI ACNNNNGTAYC 6 cut(s) 843, 876, 940, 973, 1360, 1393
BanII GRGCYC 2 cut(s) 166, 1050
BbsI GAAGAC 3 cut(s) 201, 222, 767
Bbv12I GWGCWC 2 cut(s) 166, 173
BbvI GCAGC 2 cut(s) 114, 874
BccI CCATC 2 cut(s) 170, 194
BceAI ACGGC 1 cut(s) 870
BciT130I CCWGG 1 cut(s) 465
BcoDI GTCTC 1 cut(s) 1023
BfaI CTAG 2 cut(s) 212, 1476
BfmI CTRYAG 1 cut(s) 1383
BglII AGATCT 1 cut(s) 1578
BisI GCNGC 2 cut(s) 128, 888
BlsI GCNGC 2 cut(s) 129, 889
Bme1390I CCNGG 1 cut(s) 465
Bme18I GGWCC 1 cut(s) 1388
BmeT110I CYCGRG 1 cut(s) 396
BmgT120I GGNCC 2 cut(s) 392, 1388
BmrFI CCNGG 1 cut(s) 465
BmsI GCATC 3 cut(s) 1157, 1316, 1496
BpiI GAAGAC 3 cut(s) 201, 222, 767
BpuEI CTTGAG 2 cut(s) 625, 1539
Bsa29I ATCGAT 1 cut(s) 1566
BsaHI GRCGYC 1 cut(s) 1164
BsaJI CCNNGG 1 cut(s) 463
Bse1I ACTGG 2 cut(s) 1391, 1519
Bse21I CCTNAGG 1 cut(s) 1168
Bse3DI GCAATG 2 cut(s) 1365, 1526
BseBI CCWGG 1 cut(s) 465
BseCI ATCGAT 1 cut(s) 1566
BseDI CCNNGG 1 cut(s) 463
BseGI GGATG 2 cut(s) 1413, 1473
BseMI GCAATG 2 cut(s) 1365, 1526
BseMII CTCAG 4 cut(s) 179, 1182, 1386, 1439
BseNI ACTGG 2 cut(s) 1391, 1519
BseRI GAGGAG 2 cut(s) 384, 660
BseSI GKGCMC 1 cut(s) 464
BseXI GCAGC 2 cut(s) 114, 874
BseYI CCCAGC 1 cut(s) 1364
BsgI GTGCAG 3 cut(s) 759, 873, 1365
Bsh1236I CGCG 1 cut(s) 618
BshFI GGCC 1 cut(s) 394
BshVI ATCGAT 1 cut(s) 1566
BsiHKAI GWGCWC 2 cut(s) 166, 173
BsiHKCI CYCGRG 1 cut(s) 396
BslFI GGGAC 1 cut(s) 739
BsmAI GTCTC 1 cut(s) 1023
BsmFI GGGAC 1 cut(s) 739
BsmI GAATGC 2 cut(s) 537, 795
BsnI GGCC 1 cut(s) 394
BsoBI CYCGRG 1 cut(s) 396
Bsp1286I GDGCHC 4 cut(s) 166, 173, 464, 1050
Bsp143I GATC 5 cut(s) 416, 1222, 1539, 1563, 1578
BspACI CCGC 3 cut(s) 618, 785, 857
BspANI GGCC 1 cut(s) 394
BspCNI CTCAG 4 cut(s) 178, 1181, 1385, 1438
BspDI ATCGAT 1 cut(s) 1566
BspFNI CGCG 1 cut(s) 618
BspPI GGATC 1 cut(s) 1230
BsrDI GCAATG 2 cut(s) 1365, 1526
BsrI ACTGG 2 cut(s) 1391, 1519
BssECI CCNNGG 1 cut(s) 463
BssMI GATC 5 cut(s) 416, 1222, 1539, 1563, 1578
BssNI GRCGYC 1 cut(s) 1164
Bst2UI CCWGG 1 cut(s) 465
Bst4CI ACNGT 6 cut(s) 255, 478, 953, 1384, 1400, 1450
Bst6I CTCTTC 3 cut(s) 99, 1335, 1588
BstACI GRCGYC 1 cut(s) 1164
BstC8I GCNNGC 4 cut(s) 387, 436, 1142, 1146
BstDEI CTNAG 5 cut(s) 165, 801, 1168, 1372, 1425
BstF5I GGATG 2 cut(s) 1413, 1473
BstFNI CGCG 1 cut(s) 618
BstHHI GCGC 1 cut(s) 337
BstKTI GATC 5 cut(s) 419, 1225, 1542, 1566, 1581
BstMAI GTCTC 1 cut(s) 1023
BstMBI GATC 5 cut(s) 416, 1222, 1539, 1563, 1578
BstMWI GCNNNNNNNGC 7 cut(s) 391, 875, 884, 971, 980, 1112, 1343
BstNI CCWGG 1 cut(s) 465
BstNSI RCATGY 3 cut(s) 896, 1144, 1409
BstSCI CCNGG 1 cut(s) 463
BstSFI CTRYAG 1 cut(s) 1383
BstSLI GKGCMC 1 cut(s) 464
BstUI CGCG 1 cut(s) 618
BstV1I GCAGC 2 cut(s) 114, 874
BstV2I GAAGAC 3 cut(s) 201, 222, 767
BstX2I RGATCY 1 cut(s) 1578
BstYI RGATCY 1 cut(s) 1578
Bsu15I ATCGAT 1 cut(s) 1566
Bsu36I CCTNAGG 1 cut(s) 1168
BsuRI GGCC 1 cut(s) 394
BsuTUI ATCGAT 1 cut(s) 1566
BtsCI GGATG 2 cut(s) 1413, 1473
BtsIMutI CAGTG 4 cut(s) 129, 173, 958, 1380
Cac8I GCNNGC 4 cut(s) 387, 436, 1142, 1146
CfoI GCGC 1 cut(s) 337
Cfr13I GGNCC 2 cut(s) 392, 1388
ClaI ATCGAT 1 cut(s) 1566
CseI GACGC 2 cut(s) 736, 1172
Csp6I GTAC 4 cut(s) 374, 409, 1264, 1369
CviQI GTAC 4 cut(s) 374, 409, 1264, 1369
DdeI CTNAG 5 cut(s) 165, 801, 1168, 1372, 1425
DpnI GATC 5 cut(s) 418, 1224, 1541, 1565, 1580
DpnII GATC 5 cut(s) 416, 1222, 1539, 1563, 1578
Eam1104I CTCTTC 3 cut(s) 99, 1335, 1588
EarI CTCTTC 3 cut(s) 99, 1335, 1588
Ecl136II GAGCTC 1 cut(s) 164
Eco24I GRGCYC 2 cut(s) 166, 1050
Eco47I GGWCC 1 cut(s) 1388
Eco53kI GAGCTC 1 cut(s) 164
Eco57I CTGAAG 2 cut(s) 397, 1128
Eco81I CCTNAGG 1 cut(s) 1168
Eco88I CYCGRG 1 cut(s) 396
EcoICRI GAGCTC 1 cut(s) 164
EcoO109I RGGNCCY 1 cut(s) 392
EcoRII CCWGG 1 cut(s) 463
EcoT38I GRGCYC 2 cut(s) 166, 1050
FalI AAGNNNNNCTT 2 cut(s) 1211, 1243
FaqI GGGAC 1 cut(s) 739
FauNDI CATATG 1 cut(s) 289
FblI GTMKAC 1 cut(s) 1402
Fnu4HI GCNGC 2 cut(s) 128, 888
FokI GGATG 2 cut(s) 1400, 1480
FriOI GRGCYC 2 cut(s) 166, 1050
Fsp4HI GCNGC 2 cut(s) 128, 888
FspBI CTAG 2 cut(s) 212, 1476
FspI TGCGCA 1 cut(s) 336
GlaI GCGC 1 cut(s) 336
GluI GCNGC 2 cut(s) 128, 888
GsaI CCCAGC 1 cut(s) 1368
HaeIII GGCC 1 cut(s) 394
HgaI GACGC 2 cut(s) 736, 1172
HhaI GCGC 1 cut(s) 337
Hin1I GRCGYC 1 cut(s) 1164
Hin6I GCGC 1 cut(s) 335
HinP1I GCGC 1 cut(s) 335
HincII GTYRAC 5 cut(s) 30, 601, 1206, 1396, 1458
HindII GTYRAC 5 cut(s) 30, 601, 1206, 1396, 1458
HindIII AAGCTT 2 cut(s) 6, 432
HpaI GTTAAC 3 cut(s) 601, 1396, 1458
HphI GGTGA 3 cut(s) 1441, 1462, 1529
Hpy166II GTNNAC 8 cut(s) 30, 376, 409, 601, 1206, 1396, 1403, 1458
Hpy188I TCNGA 8 cut(s) 161, 175, 322, 416, 421, 1153, 1242, 1312
Hpy8I GTNNAC 8 cut(s) 30, 376, 409, 601, 1206, 1396, 1403, 1458
HpyAV CCTTC 6 cut(s) 69, 83, 349, 1028, 1103, 1514
HpyCH4III ACNGT 6 cut(s) 255, 478, 953, 1384, 1400, 1450
HpyCH4IV ACGT 3 cut(s) 69, 219, 1277
HpyF10VI GCNNNNNNNGC 7 cut(s) 391, 875, 884, 971, 980, 1112, 1343
HpyF3I CTNAG 5 cut(s) 165, 801, 1168, 1372, 1425
HpySE526I ACGT 3 cut(s) 69, 219, 1277
Hsp92I GRCGYC 1 cut(s) 1164
HspAI GCGC 1 cut(s) 335
KspAI GTTAAC 3 cut(s) 601, 1396, 1458
Kzo9I GATC 5 cut(s) 416, 1222, 1539, 1563, 1578
LmnI GCTCC 3 cut(s) 1112, 1355, 1523
Lsp1109I GCAGC 2 cut(s) 114, 874
LweI GCATC 3 cut(s) 1157, 1316, 1496
MaeI CTAG 2 cut(s) 212, 1476
MaeII ACGT 3 cut(s) 69, 219, 1277
MaeIII GTNAC 4 cut(s) 442, 1301, 1429, 1438
MalI GATC 5 cut(s) 418, 1224, 1541, 1565, 1580
MboI GATC 5 cut(s) 416, 1222, 1539, 1563, 1578
MfeI CAATTG 1 cut(s) 807
MflI RGATCY 1 cut(s) 1578
MhlI GDGCHC 4 cut(s) 166, 173, 464, 1050
MluCI AATT 5 cut(s) 107, 236, 489, 807, 1419
MlyI GAGTC 6 cut(s) 147, 1025, 1065, 1173, 1201, 1473
MroXI GAANNNNTTC 1 cut(s) 219
MseI TTAA 8 cut(s) 54, 300, 522, 600, 882, 933, 1395, 1457
MspR9I CCNGG 1 cut(s) 465
MunI CAATTG 1 cut(s) 807
Mva1269I GAATGC 2 cut(s) 537, 795
MvaI CCWGG 1 cut(s) 465
MvnI CGCG 1 cut(s) 618
MwoI GCNNNNNNNGC 7 cut(s) 391, 875, 884, 971, 980, 1112, 1343
NdeI CATATG 1 cut(s) 289
NdeII GATC 5 cut(s) 416, 1222, 1539, 1563, 1578
NmeAIII GCCGAG 1 cut(s) 513
NmuCI GTSAC 3 cut(s) 442, 1429, 1438
NsbI TGCGCA 1 cut(s) 336
NspI RCATGY 3 cut(s) 896, 1144, 1409
PaeI GCATGC 1 cut(s) 1144
PaeR7I CTCGAG 1 cut(s) 396
PciI ACATGT 1 cut(s) 892
PctI GAATGC 2 cut(s) 537, 795
PdmI GAANNNNTTC 1 cut(s) 219
PfeI GAWTC 5 cut(s) 135, 180, 468, 985, 1009
PflFI GACNNNGTC 1 cut(s) 1316
PkrI GCNGC 2 cut(s) 129, 889
PleI GAGTC 6 cut(s) 147, 1024, 1065, 1173, 1201, 1473
PpsI GAGTC 6 cut(s) 147, 1024, 1065, 1173, 1201, 1473
PscI ACATGT 1 cut(s) 892
PshBI ATTAAT 1 cut(s) 882
Psp124BI GAGCTC 1 cut(s) 166
Psp6I CCWGG 1 cut(s) 463
PspFI CCCAGC 1 cut(s) 1364
PspGI CCWGG 1 cut(s) 463
PspPI GGNCC 2 cut(s) 392, 1388
PsuI RGATCY 1 cut(s) 1578
PsyI GACNNNGTC 1 cut(s) 1316
RsaI GTAC 4 cut(s) 375, 410, 1265, 1370
RsaNI GTAC 4 cut(s) 374, 409, 1264, 1369
SacI GAGCTC 1 cut(s) 166
SaqAI TTAA 8 cut(s) 54, 300, 522, 600, 882, 933, 1395, 1457
SatI GCNGC 2 cut(s) 128, 888
Sau3AI GATC 5 cut(s) 416, 1222, 1539, 1563, 1578
Sau96I GGNCC 2 cut(s) 392, 1388
SchI GAGTC 6 cut(s) 147, 1025, 1065, 1173, 1201, 1473
ScrFI CCNGG 1 cut(s) 465
SduI GDGCHC 4 cut(s) 166, 173, 464, 1050
SfaNI GCATC 3 cut(s) 1157, 1316, 1496
SfcI CTRYAG 1 cut(s) 1383
Sfr274I CTCGAG 1 cut(s) 396
SinI GGWCC 1 cut(s) 1388
SlaI CTCGAG 1 cut(s) 396
SmlI CTYRAG 3 cut(s) 396, 640, 1554
SmoI CTYRAG 3 cut(s) 396, 640, 1554
SphI GCATGC 1 cut(s) 1144
Sse9I AATT 5 cut(s) 107, 236, 489, 807, 1419
SsiI CCGC 3 cut(s) 618, 785, 857
SspI AATATT 3 cut(s) 43, 343, 679
SspMI CTAG 2 cut(s) 212, 1476
SstI GAGCTC 1 cut(s) 166
StyD4I CCNGG 1 cut(s) 463
TaaI ACNGT 6 cut(s) 255, 478, 953, 1384, 1400, 1450
TaiI ACGT 3 cut(s) 72, 222, 1280
TaqI TCGA 7 cut(s) 11, 133, 397, 903, 1490, 1542, 1566
TasI AATT 5 cut(s) 107, 236, 489, 807, 1419
TatI WGTACW 3 cut(s) 373, 408, 1263
TfiI GAWTC 5 cut(s) 135, 180, 468, 985, 1009
Tru1I TTAA 8 cut(s) 54, 300, 522, 600, 882, 933, 1395, 1457
Tru9I TTAA 8 cut(s) 54, 300, 522, 600, 882, 933, 1395, 1457
TscAI CASTG 4 cut(s) 129, 173, 958, 1387
TseFI GTSAC 3 cut(s) 442, 1429, 1438
TseI GCWGC 2 cut(s) 127, 887
Tsp45I GTSAC 3 cut(s) 442, 1429, 1438
TspDTI ATGAA 6 cut(s) 438, 593, 599, 1274, 1340, 1589
TspGWI ACGGA 1 cut(s) 1448
TspRI CASTG 4 cut(s) 129, 173, 958, 1387
Tth111I GACNNNGTC 1 cut(s) 1316
VpaK11BI GGWCC 1 cut(s) 1388
VspI ATTAAT 1 cut(s) 882
XapI RAATTY 1 cut(s) 236
XceI RCATGY 3 cut(s) 896, 1144, 1409
XhoI CTCGAG 1 cut(s) 396
XmiI GTMKAC 1 cut(s) 1402
XmnI GAANNNNTTC 1 cut(s) 219
XspI CTAG 2 cut(s) 212, 1476
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.