FvH4_7g26950

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0008946
Physical Location & Seq
Reverse (-)
20232187 .. 20238141
5955 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g26950.t2

Sequence Viewer

Length: 1581 bp
ATGGAATCCGCCATTTCCTTATCTTCTTCCTCTCTTCTCCTACCTTCGCCTCGCCGCCGGCTCGGCTCGAACTCACAAGCCTCAATCTCGCTCCGGAACCTCCGCCGTCCGAGCCTCAAATCCAGAAGCTTCCTCGCTCCGTCTCTTTCCCGCCAAAACCGGCCAATCCGATCTCAGAGCTCCAATCATCAGCCGGTCGACGACGGCTTCGTCCTCGAAGACGTTCCTCACCTCACCGATTTCCTCCCAAACTTGCCGTCATATCCGAATCCACTTCAACAGAGCCAAGCTTATGCAGTTGTCAAGCAAACTTTTGTGAGCCCGGCGGATGCGGTTGCGCAGAAAGTTGTTGTTCAAGCTGATAGTCCTAGAGGGGTGCATTTCCGGCGAGCAGGGCCTCGGGAAAAGGTTTATTTTAAGTCGGAGGAAGTGCGTGCCTGTATAGTAACGTGTGGAGGTTTATGCCCCGGGATCAACACTGTGATTCGAGAGATAGTATGCGGCTTGAACTACATGTATGGTGTTGAGGATATACTTGGAATTGAGGGAGGATATAGAGGTTTTTATTCAAAAAATACATTAAAGTTGAATCCGAAAGTTGTCAATGATATCCATAAGAAGGGTGGCACCTTTCTTCGCACTTCACGAGGAGGTCATGATACCAACAAGATTGTTGATAACATACAGGACCGGGGAATAAATCAGGTATACATAATTGGAGGTGATGGTACCCAAAAAGGAGCAGCTCTAATATATAAGGAAGTTGAGAAACGTGGTCTTCAAGTGGCAGTTGCTGGGGTTCCTAAAACAATTGATAATGATATTGCTGTGATAGACAAATCTTTTGGTTTTGACACTGCTGTTGAAGAAGCTCAGAGGGCTATTAATGCTGCACATGTCGAAGTTGAAAGTGTAGATAATGGAATCGGAATTGTCAAACTGATGGGAAGATACAGTGGCTTCATTGCTTCATATGCAACTTTGGCAAGTCGAGATGTGGATTGTTGCTTGATTCCAGAATCTCCATTCTATTTGGAAGGCCAGGGTGGACTTTTCGAATTTATTGAACAGCGTCTTAAAGAAAATGGACATATGGTTATCGTAGTAGCAGAAGGAGCAGGACAGGAGTATGTTGCCCAGCAAATGCAGGCTGCTGGACAGAAAGATGCATCCGGAAATAAGCTGCTCCTAGACGTTGGTCTATGGTTAACCCAAAAGATTAAGGATCATTTTGCAAAAGGTCAGCAGATGGCAATAAATATGAAGTATATAGATCCAACATATATGATACGAGCTATTCCAAGTAATGCATCAGACAATATATACTGCACTCTTTTGGCTCATAGTGCTGTTCATGGGGCCATGGCTGGATATAGTGGTTTCACGGTTGGGCCTGTTAACAGCAGACATGCCTACATTCCAATTTCTTGTGTGACAGAGACGCAAAACACAGTGAACTTGACAGATCGAATGTGGGCTAGACTTCTTGCATCAACGAACCAGCCTAGTTTCTTGGAGAGAAATGAGTTTCAGGATGGAGTTGATTTGATGAACAGCAACATGGAAAATACCTCTATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003872 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008443 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009507 GO:0009536 GO:0009987 GO:0016052 GO:0016053 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019200 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044262 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046835 GO:0046939 GO:0051186 GO:0051188 GO:0055086 GO:0061615 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00030 ko00051 ko00052 ko00680 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko03018 ko04152 ko05230 map00010 map00030 map00051 map00052 map00680 map01100 map01110 map01120 map01130 map01200 map01230 map03018 map04152 map05230
Pfam Domains
Protein Families
PFK

Protein Analysis

527

Amino Acids

57.58

Weight (kDa)

8.02

Isoelectric Point (pI)

44.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PFK PF00365 146 - 452 2.6e-61 Phosphofructokinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 209
Acc16I TGCGCA 1 cut(s) 339
Acc65I GGTACC 1 cut(s) 728
AccB1I GGYRCC 2 cut(s) 626, 728
AccI GTMKAC 2 cut(s) 198, 708
AccIII TCCGGA 2 cut(s) 93, 1172
AciI CCGC 7 cut(s) 9, 55, 103, 151, 326, 332, 501
AclWI GGATC 3 cut(s) 479, 1233, 1268
AcoI YGGCCR 1 cut(s) 161
AcsI RAATTY 1 cut(s) 1058
AfaI GTAC 1 cut(s) 730
AfiI CCNNNNNNNGG 1 cut(s) 619
AflIII ACRYGT 3 cut(s) 449, 513, 895
AgsI TTSAA 9 cut(s) 278, 356, 508, 570, 589, 782, 866, 908, 1067
AjnI CCWGG 1 cut(s) 1041
AluBI AGCT 8 cut(s) 129, 180, 290, 359, 746, 872, 1183, 1295
AluI AGCT 8 cut(s) 129, 180, 290, 359, 746, 872, 1183, 1295
Alw21I GWGCWC 1 cut(s) 182
Alw26I GTCTC 2 cut(s) 147, 1433
AlwI GGATC 3 cut(s) 479, 1233, 1268
AlwNI CAGNNNCTG 1 cut(s) 794
Ama87I CYCGRG 2 cut(s) 399, 467
Aor13HI TCCGGA 2 cut(s) 93, 1172
AoxI GGCC 5 cut(s) 161, 395, 1039, 1359, 1391
ApeKI GCWGC 4 cut(s) 743, 890, 1151, 1183
ApoI RAATTY 1 cut(s) 1058
ArsI GACNNNNNNTTYG 6 cut(s) 191, 223, 242, 274, 827, 859
AseI ATTAAT 1 cut(s) 885
Asp700I GAANNNNTTC 1 cut(s) 222
Asp718I GGTACC 1 cut(s) 728
AspLEI GCGC 1 cut(s) 340
AspS9I GGNCC 4 cut(s) 395, 688, 1359, 1391
AsuC2I CCSGG 4 cut(s) 323, 468, 469, 692
AsuHPI GGTGA 3 cut(s) 221, 226, 734
AsuII TTCGAA 1 cut(s) 1056
AvaI CYCGRG 2 cut(s) 399, 467
AvaII GGWCC 1 cut(s) 688
BanI GGYRCC 2 cut(s) 626, 728
BanII GRGCYC 2 cut(s) 182, 323
BauI CACGAG 1 cut(s) 645
BbsI GAAGAC 2 cut(s) 225, 770
Bbv12I GWGCWC 1 cut(s) 182
BbvI GCAGC 4 cut(s) 755, 877, 1138, 1170
BccI CCATC 4 cut(s) 719, 937, 1243, 1529
BceAI ACGGC 3 cut(s) 90, 220, 241
BciT130I CCWGG 1 cut(s) 1043
BcnI CCSGG 4 cut(s) 323, 468, 469, 692
BcoDI GTCTC 2 cut(s) 147, 1433
BfaI CTAG 4 cut(s) 369, 1190, 1479, 1506
BglI GCCNNNNNGGC 1 cut(s) 63
BisI GCNGC 6 cut(s) 55, 502, 744, 891, 1152, 1184
BlsI GCNGC 6 cut(s) 56, 503, 745, 892, 1153, 1185
Bme1390I CCNGG 5 cut(s) 323, 468, 469, 692, 1043
Bme18I GGWCC 1 cut(s) 688
BmeT110I CYCGRG 2 cut(s) 399, 467
BmgT120I GGNCC 4 cut(s) 395, 688, 1359, 1391
BmiI GGNNCC 5 cut(s) 98, 628, 730, 801, 1360
BmrFI CCNGG 5 cut(s) 323, 468, 469, 692, 1043
BmsI GCATC 5 cut(s) 319, 1156, 1178, 1319, 1499
BoxI GACNNNNGTC 1 cut(s) 1197
BpiI GAAGAC 2 cut(s) 225, 770
Bpu14I TTCGAA 1 cut(s) 1056
BpuMI CCSGG 4 cut(s) 323, 468, 469, 692
BsaJI CCNNGG 6 cut(s) 398, 466, 467, 691, 1042, 1362
BsaWI WCCGGW 2 cut(s) 93, 1172
Bsc4I CCNNNNNNNGG 1 cut(s) 619
Bse118I RCCGGY 3 cut(s) 57, 159, 193
Bse3DI GCAATG 1 cut(s) 963
BseAI TCCGGA 2 cut(s) 93, 1172
BseBI CCWGG 1 cut(s) 1043
BseDI CCNNGG 6 cut(s) 398, 466, 467, 691, 1042, 1362
BseGI GGATG 3 cut(s) 334, 1169, 1540
BseLI CCNNNNNNNGG 1 cut(s) 619
BseMI GCAATG 1 cut(s) 963
BseMII CTCAG 2 cut(s) 188, 887
BseRI GAGGAG 1 cut(s) 663
BseXI GCAGC 4 cut(s) 755, 877, 1138, 1170
BseYI CCCAGC 2 cut(s) 794, 1137
BsgI GTGCAG 2 cut(s) 876, 1312
Bsh1285I CGRYCG 1 cut(s) 198
BshFI GGCC 5 cut(s) 163, 397, 1041, 1361, 1393
BshNI GGYRCC 2 cut(s) 626, 728
BsiEI CGRYCG 1 cut(s) 198
BsiHKAI GWGCWC 1 cut(s) 182
BsiHKCI CYCGRG 2 cut(s) 399, 467
BsiSI CCGG 9 cut(s) 58, 94, 160, 194, 323, 385, 468, 691, 1173
BslI CCNNNNNNNGG 1 cut(s) 619
BsmAI GTCTC 2 cut(s) 147, 1433
BsmBI CGTCTC 2 cut(s) 147, 1433
BsnI GGCC 5 cut(s) 163, 397, 1041, 1361, 1393
BsoBI CYCGRG 2 cut(s) 399, 467
Bsp119I TTCGAA 1 cut(s) 1056
Bsp1286I GDGCHC 2 cut(s) 182, 323
Bsp13I TCCGGA 2 cut(s) 93, 1172
Bsp143I GATC 5 cut(s) 170, 471, 1225, 1273, 1465
Bsp19I CCATGG 1 cut(s) 1362
BspACI CCGC 7 cut(s) 9, 55, 103, 151, 326, 332, 501
BspANI GGCC 5 cut(s) 163, 397, 1041, 1361, 1393
BspCNI CTCAG 2 cut(s) 187, 886
BspEI TCCGGA 2 cut(s) 93, 1172
BspHI TCATGA 1 cut(s) 655
BspLI GGNNCC 5 cut(s) 98, 628, 730, 801, 1360
BspPI GGATC 3 cut(s) 479, 1233, 1268
BspT104I TTCGAA 1 cut(s) 1056
BspT107I GGYRCC 2 cut(s) 626, 728
BsrDI GCAATG 1 cut(s) 963
BsrFI RCCGGY 3 cut(s) 57, 159, 193
BssAI RCCGGY 3 cut(s) 57, 159, 193
BssECI CCNNGG 6 cut(s) 398, 466, 467, 691, 1042, 1362
BssMI GATC 5 cut(s) 170, 471, 1225, 1273, 1465
BssNAI GTATAC 1 cut(s) 709
BssSI CACGAG 1 cut(s) 645
BssT1I CCWWGG 1 cut(s) 1362
Bst1107I GTATAC 1 cut(s) 709
Bst2BI CACGAG 1 cut(s) 645
Bst2UI CCWGG 1 cut(s) 1043
Bst4CI ACNGT 4 cut(s) 481, 956, 1387, 1453
Bst6I CTCTTC 1 cut(s) 39
BstBI TTCGAA 1 cut(s) 1056
BstC8I GCNNGC 4 cut(s) 59, 390, 435, 1149
BstDEI CTNAG 2 cut(s) 174, 873
BstDSI CCRYGG 1 cut(s) 1362
BstF5I GGATG 3 cut(s) 334, 1169, 1540
BstHHI GCGC 1 cut(s) 340
BstKTI GATC 5 cut(s) 173, 474, 1228, 1276, 1468
BstMAI GTCTC 2 cut(s) 147, 1433
BstMBI GATC 5 cut(s) 170, 471, 1225, 1273, 1465
BstMCI CGRYCG 1 cut(s) 198
BstNI CCWGG 1 cut(s) 1043
BstNSI RCATGY 3 cut(s) 517, 899, 1412
BstPAI GACNNNNGTC 1 cut(s) 1197
BstSCI CCNGG 5 cut(s) 321, 466, 467, 690, 1041
BstV1I GCAGC 4 cut(s) 755, 877, 1138, 1170
BstV2I GAAGAC 2 cut(s) 225, 770
BstX2I RGATCY 1 cut(s) 1273
BstYI RGATCY 1 cut(s) 1273
BstZ17I GTATAC 1 cut(s) 709
BsuRI GGCC 5 cut(s) 163, 397, 1041, 1361, 1393
BtgI CCRYGG 1 cut(s) 1362
BtsCI GGATG 3 cut(s) 334, 1169, 1540
BtsI GCAGTG 1 cut(s) 855
BtsIMutI CAGTG 4 cut(s) 477, 855, 961, 1458
Cac8I GCNNGC 4 cut(s) 59, 390, 435, 1149
CaiI CAGNNNCTG 1 cut(s) 794
CciI TCATGA 1 cut(s) 655
CfoI GCGC 1 cut(s) 340
Cfr10I RCCGGY 3 cut(s) 57, 159, 193
Cfr13I GGNCC 4 cut(s) 395, 688, 1359, 1391
Cfr9I CCCGGG 1 cut(s) 467
CseI GACGC 2 cut(s) 1061, 1450
Csp6I GTAC 1 cut(s) 729
CviAII CATG 7 cut(s) 514, 656, 896, 1355, 1363, 1409, 1561
CviQI GTAC 1 cut(s) 729
DdeI CTNAG 2 cut(s) 174, 873
DpnI GATC 5 cut(s) 172, 473, 1227, 1275, 1467
DpnII GATC 5 cut(s) 170, 471, 1225, 1273, 1465
DrdI GACNNNNNNGTC 1 cut(s) 209
DseDI GACNNNNNNGTC 1 cut(s) 209
EaeI YGGCCR 1 cut(s) 161
Eam1104I CTCTTC 1 cut(s) 39
EarI CTCTTC 1 cut(s) 39
EciI GGCGGA 2 cut(s) 92, 341
Ecl136II GAGCTC 1 cut(s) 180
Eco130I CCWWGG 1 cut(s) 1362
Eco24I GRGCYC 2 cut(s) 182, 323
Eco32I GATATC 1 cut(s) 610
Eco47I GGWCC 1 cut(s) 688
Eco53kI GAGCTC 1 cut(s) 180
Eco88I CYCGRG 2 cut(s) 399, 467
EcoICRI GAGCTC 1 cut(s) 180
EcoO109I RGGNCCY 1 cut(s) 395
EcoRII CCWGG 1 cut(s) 1041
EcoRV GATATC 1 cut(s) 610
EcoT14I CCWWGG 1 cut(s) 1362
EcoT22I ATGCAT 2 cut(s) 1171, 1312
EcoT38I GRGCYC 2 cut(s) 182, 323
ErhI CCWWGG 1 cut(s) 1362
Esp3I CGTCTC 2 cut(s) 147, 1433
FaeI CATG 7 cut(s) 517, 659, 899, 1358, 1366, 1412, 1564
FatI CATG 7 cut(s) 513, 655, 895, 1354, 1362, 1408, 1560
FauI CCCGC 1 cut(s) 158
FauNDI CATATG 2 cut(s) 973, 1092
FblI GTMKAC 2 cut(s) 198, 708
Fnu4HI GCNGC 6 cut(s) 55, 502, 744, 891, 1152, 1184
FokI GGATG 3 cut(s) 341, 1156, 1547
FriOI GRGCYC 2 cut(s) 182, 323
Fsp4HI GCNGC 6 cut(s) 55, 502, 744, 891, 1152, 1184
FspBI CTAG 4 cut(s) 369, 1190, 1479, 1506
FspI TGCGCA 1 cut(s) 339
GlaI GCGC 1 cut(s) 339
GluI GCNGC 6 cut(s) 55, 502, 744, 891, 1152, 1184
GsaI CCCAGC 2 cut(s) 798, 1141
HaeIII GGCC 5 cut(s) 163, 397, 1041, 1361, 1393
HapII CCGG 9 cut(s) 58, 94, 160, 194, 323, 385, 468, 691, 1173
HgaI GACGC 2 cut(s) 1061, 1450
HhaI GCGC 1 cut(s) 340
Hin1II CATG 7 cut(s) 517, 659, 899, 1358, 1366, 1412, 1564
Hin6I GCGC 1 cut(s) 338
HinP1I GCGC 1 cut(s) 338
HincII GTYRAC 3 cut(s) 199, 1209, 1399
HindII GTYRAC 3 cut(s) 199, 1209, 1399
HindIII AAGCTT 2 cut(s) 127, 288
HinfI GANTC 7 cut(s) 5, 268, 484, 589, 924, 1012, 1019
HpaI GTTAAC 2 cut(s) 1209, 1399
HpaII CCGG 9 cut(s) 58, 94, 160, 194, 323, 385, 468, 691, 1173
HphI GGTGA 3 cut(s) 221, 226, 734
Hpy166II GTNNAC 6 cut(s) 199, 709, 1049, 1209, 1399, 1456
Hpy188I TCNGA 9 cut(s) 111, 170, 177, 267, 424, 594, 876, 929, 1315
Hpy8I GTNNAC 6 cut(s) 199, 709, 1049, 1209, 1399, 1456
Hpy99I CGWCG 2 cut(s) 203, 206
HpyAV CCTTC 4 cut(s) 54, 613, 1031, 1106
HpyCH4III ACNGT 4 cut(s) 481, 956, 1387, 1453
HpyCH4IV ACGT 4 cut(s) 222, 449, 772, 1194
HpyF3I CTNAG 2 cut(s) 174, 873
HpySE526I ACGT 4 cut(s) 222, 449, 772, 1194
Hsp92II CATG 7 cut(s) 517, 659, 899, 1358, 1366, 1412, 1564
HspAI GCGC 1 cut(s) 338
Kpn2I TCCGGA 2 cut(s) 93, 1172
KpnI GGTACC 1 cut(s) 732
KroI GCCGGC 1 cut(s) 57
KroNI GCCGGC 1 cut(s) 59
KspAI GTTAAC 2 cut(s) 1209, 1399
Kzo9I GATC 5 cut(s) 170, 471, 1225, 1273, 1465
LmnI GCTCC 6 cut(s) 96, 142, 185, 740, 1115, 1191
Lsp1109I GCAGC 4 cut(s) 755, 877, 1138, 1170
LweI GCATC 5 cut(s) 319, 1156, 1178, 1319, 1499
MaeI CTAG 4 cut(s) 369, 1190, 1479, 1506
MaeII ACGT 4 cut(s) 222, 449, 772, 1194
MaeIII GTNAC 2 cut(s) 445, 1432
MalI GATC 5 cut(s) 172, 473, 1227, 1275, 1467
MboI GATC 5 cut(s) 170, 471, 1225, 1273, 1465
MboII GAAGA 8 cut(s) 15, 18, 26, 230, 626, 770, 878, 960
MfeI CAATTG 1 cut(s) 810
MflI RGATCY 1 cut(s) 1273
MhlI GDGCHC 2 cut(s) 182, 323
MluCI AATT 6 cut(s) 540, 714, 810, 930, 1058, 1422
MmeI TCCRAC 2 cut(s) 402, 1301
Mph1103I ATGCAT 2 cut(s) 1171, 1312
MroI TCCGGA 2 cut(s) 93, 1172
MroNI GCCGGC 1 cut(s) 57
MroXI GAANNNNTTC 1 cut(s) 222
MseI TTAA 7 cut(s) 417, 581, 885, 1077, 1208, 1221, 1398
MspI CCGG 9 cut(s) 58, 94, 160, 194, 323, 385, 468, 691, 1173
MspR9I CCNGG 5 cut(s) 323, 468, 469, 692, 1043
MunI CAATTG 1 cut(s) 810
MvaI CCWGG 1 cut(s) 1043
NaeI GCCGGC 1 cut(s) 59
NciI CCSGG 4 cut(s) 323, 468, 469, 692
NcoI CCATGG 1 cut(s) 1362
NdeI CATATG 2 cut(s) 973, 1092
NdeII GATC 5 cut(s) 170, 471, 1225, 1273, 1465
NgoMIV GCCGGC 1 cut(s) 57
NlaIII CATG 7 cut(s) 517, 659, 899, 1358, 1366, 1412, 1564
NlaIV GGNNCC 5 cut(s) 98, 628, 730, 801, 1360
NmeAIII GCCGAG 1 cut(s) 42
NmuCI GTSAC 1 cut(s) 1432
NsbI TGCGCA 1 cut(s) 339
NsiI ATGCAT 2 cut(s) 1171, 1312
NspI RCATGY 3 cut(s) 517, 899, 1412
NspV TTCGAA 1 cut(s) 1056
PagI TCATGA 1 cut(s) 655
PciI ACATGT 2 cut(s) 513, 895
PcsI WCGNNNNNNNCGW 2 cut(s) 207, 643
PdiI GCCGGC 1 cut(s) 59
PdmI GAANNNNTTC 1 cut(s) 222
PfeI GAWTC 7 cut(s) 5, 268, 484, 589, 924, 1012, 1019
PkrI GCNGC 6 cut(s) 56, 503, 745, 892, 1153, 1185
PscI ACATGT 2 cut(s) 513, 895
PshAI GACNNNNGTC 1 cut(s) 1197
PshBI ATTAAT 1 cut(s) 885
Psp124BI GAGCTC 1 cut(s) 182
Psp6I CCWGG 1 cut(s) 1041
PspFI CCCAGC 2 cut(s) 794, 1137
PspGI CCWGG 1 cut(s) 1041
PspN4I GGNNCC 5 cut(s) 98, 628, 730, 801, 1360
PspPI GGNCC 4 cut(s) 395, 688, 1359, 1391
PstNI CAGNNNCTG 1 cut(s) 794
PsuI RGATCY 1 cut(s) 1273
RsaI GTAC 1 cut(s) 730
RsaNI GTAC 1 cut(s) 729
SacI GAGCTC 1 cut(s) 182
SalI GTCGAC 1 cut(s) 197
SaqAI TTAA 7 cut(s) 417, 581, 885, 1077, 1208, 1221, 1398
SatI GCNGC 6 cut(s) 55, 502, 744, 891, 1152, 1184
Sau3AI GATC 5 cut(s) 170, 471, 1225, 1273, 1465
Sau96I GGNCC 4 cut(s) 395, 688, 1359, 1391
ScrFI CCNGG 5 cut(s) 323, 468, 469, 692, 1043
SduI GDGCHC 2 cut(s) 182, 323
SfaNI GCATC 5 cut(s) 319, 1156, 1178, 1319, 1499
SfuI TTCGAA 1 cut(s) 1056
SinI GGWCC 1 cut(s) 688
SmaI CCCGGG 1 cut(s) 469
Sse9I AATT 6 cut(s) 540, 714, 810, 930, 1058, 1422
SsiI CCGC 7 cut(s) 9, 55, 103, 151, 326, 332, 501
SspMI CTAG 4 cut(s) 369, 1190, 1479, 1506
SstI GAGCTC 1 cut(s) 182
StyD4I CCNGG 5 cut(s) 321, 466, 467, 690, 1041
StyI CCWWGG 1 cut(s) 1362
TaaI ACNGT 4 cut(s) 481, 956, 1387, 1453
TaiI ACGT 4 cut(s) 225, 452, 775, 1197
TaqI TCGA 8 cut(s) 68, 198, 216, 487, 900, 991, 1056, 1468
TasI AATT 6 cut(s) 540, 714, 810, 930, 1058, 1422
TauI GCSGC 2 cut(s) 57, 504
TfiI GAWTC 7 cut(s) 5, 268, 484, 589, 924, 1012, 1019
Tru1I TTAA 7 cut(s) 417, 581, 885, 1077, 1208, 1221, 1398
Tru9I TTAA 7 cut(s) 417, 581, 885, 1077, 1208, 1221, 1398
TscAI CASTG 4 cut(s) 484, 862, 961, 1458
TseFI GTSAC 1 cut(s) 1432
TseI GCWGC 4 cut(s) 743, 890, 1151, 1183
Tsp45I GTSAC 1 cut(s) 1432
TspDTI ATGAA 5 cut(s) 952, 960, 1277, 1343, 1565
TspGWI ACGGA 1 cut(s) 129
TspMI CCCGGG 1 cut(s) 467
TspRI CASTG 4 cut(s) 484, 862, 961, 1458
VpaK11BI GGWCC 1 cut(s) 688
VspI ATTAAT 1 cut(s) 885
XapI RAATTY 1 cut(s) 1058
XceI RCATGY 3 cut(s) 517, 899, 1412
XcmI CCANNNNNNNNNTGG 1 cut(s) 620
XmaI CCCGGG 1 cut(s) 467
XmiI GTMKAC 2 cut(s) 198, 708
XmnI GAANNNNTTC 1 cut(s) 222
XspI CTAG 4 cut(s) 369, 1190, 1479, 1506
Zsp2I ATGCAT 2 cut(s) 1171, 1312
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.