Rmu_sc0013090.1_g000001

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0013090.1
OG0008946
Physical Location & Seq
Forward (+)
1 .. 9124
9124 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0013090.1_g000001.1.cds

Sequence Viewer

Length: 2714 bp
ggaagtttggagcagagcagtttgttaaattgctccccgacaaagttcttgttcattgtagtgaacggaatacctctacgctgactctggatggtgtggatgtaatgggagagcgcttggcaggagaggttgtcgaactggttcaaagaaagccaaatttacgtaagatctcattcgttgcacattctgttggagggttagtggcaagatatgcaattgggagactatatagacccgctaagggagaaaatatggaacattcacatcctaatggctgtgaagaggattcaaggagtacgatatgtggcttggagcctatgaatttcatcactgttgctacacctcatcttggttcaaggggtaacaagcaggtaccatttctttttggcgtacctgccttcgaaaaacttgctagtgcagttatccatttgatatttaggagaacaggtaggcatctgtttctcaatgacgatgacgatggcaagcctccactgcttaaacgaatgatagaagattatgatggatgttatttcatgtcggcattgcgtagtttcagacgtagggtggcatattcaaacgtgggctatgatcatattgtcggctggagaacatcatgcattaggcgtacttgtgaactgccaaagtgggaggatactgttgatgagaaatatccacatatagtttatgaagagcgctgcaaggcctatgatgctgagcagtgtgagcccacttcagcagaaggcaatgactgtgaccagctcgaaggggaactagtgacgggcctatctcgtgtgtcatgggaaaaagtagatgttagtttccactgcagcaaacggagatttgctgctcatagtgtcattcaggtaaaagatcaaactgcacataaagaaggcgaagatgttataccttatgctgttgagaaatctatgatttctgtaccactaaactacatccatcgtctcagtacaagctgggcctgcacgtttgatctcgctaccagagagccttacacaaaaaatgtgttcaaggaagcagcaggcagtcaacagaaatttaatttattcggactcccaaggctcgccatttcccagctgttctccattttcactgtaactccccctgagaatttccaaatggattccaccatttctttatcgtcttcctctcttcacctaccttcgcctagccgccgacccggctccaaatcccaagcttcaatttcgctccgtaacctcagccgtccgagcctcaaatccagaaccttcctcgttccgtctctttcccgccaaaaccggcctatccgatctcagagctcgaatcatcagccgcaggacgacggcttcgtcctcgaagacgttcctcacttgaccaatttcctcccaaacttgccgtcatatccgaatccattgcaacagagccaagcttatgcagttgtcaagaaaacttttgtgagccctgcggatgcggttgcgcagaaagttgttgttcaagatgatagtcctagaggggtacattttcgacgtgcagggcctcgggaaaaggtttatttcaagtcggaggaagtgcgtgcttgtatagtgacatgtgggggtttatgcccggggatcaatactgtgattcgagagatagtatgtggcttgagctacatgtatggtgttgaggatatacttggaattgagggaggatatagaggcttttattctaaaaatacattaaagttgacacctaaagttgtcaatgatatccataagcggggtggcacctttctctgcacttcacgaggaggtcatgataccaacaagatagttgataacatacaggaccggggaataaatcaggtatacataattggaggtgatggcacccaaaaaggagcagctctaatatatgaggaagttgagaaacgtggtcttcaagtggcagttgctggggttcctaaaacaattgacaatgatattgctgtgatagacaagtcttttggttttgacactgctgttgaagaagctcagagggctattaatgctgcacacgtggaggttgaaagtgtggaaaatggaatcggaattgtcaaactgatgggaagattcagtggcttcattgcattatatgcaactttggcaagtcgagatgtggattgttgcttgattccagaatctccattctatttggaaggccagggtgggcttttcgaatttattgaacagcgtcttaaagagaatggacatatagttatcgtagtagcagaaggagctgggcaggagtatgttgcccagcaaatccatgctgctggagagaaagatgcatctggaaataagttgctactagatgttggtatatggttaacccaaaagattaaggatcattttacaaaaggtcagcagatggcggtaaatatgaaatatatagatccaacatatatgattcgagctgttccaagtaatgcatcagacaatatatactgcactcttctggctcatagtgctgttcatggggccatggctggatacagtggtttcacggttgggcctgttaacagcagacatgcctatattccaatttctcgtgtgacagagacgcaaaacacagtgaagttgacagatcggatgtgggctagacttcttgcatcgacgaatcagcctagttttttggagagaaatgagttgcattctagagttgatctgatcaacagcaacttggaaaatacatctacataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003872 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008443 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009507 GO:0009536 GO:0009987 GO:0016052 GO:0016053 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019200 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044262 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046835 GO:0046939 GO:0051186 GO:0051188 GO:0055086 GO:0061615 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00030 ko00051 ko00052 ko00680 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko03018 ko04152 ko05230 map00010 map00030 map00051 map00052 map00680 map01100 map01110 map01120 map01130 map01200 map01230 map03018 map04152 map05230
Pfam Domains
Protein Families
PFK

Protein Analysis

903

Amino Acids

100.29

Weight (kDa)

7.62

Isoelectric Point (pI)

43.98

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1342
Acc16I TGCGCA 1 cut(s) 1472
Acc36I ACCTGC 2 cut(s) 360, 402
Acc65I GGTACC 1 cut(s) 372
AccB1I GGYRCC 3 cut(s) 372, 1759, 1861
AccI GTMKAC 1 cut(s) 1841
AciI CCGC 8 cut(s) 236, 1188, 1284, 1327, 1459, 1465, 1752, 2386
AclWI GGATC 3 cut(s) 1612, 2366, 2401
AcsI RAATTY 5 cut(s) 156, 321, 1051, 1125, 2191
AcuI CTGAAG 1 cut(s) 716
AcvI CACGTG 1 cut(s) 2031
AfaI GTAC 7 cut(s) 297, 374, 392, 627, 938, 966, 1511
AfeI AGCGCT 2 cut(s) 115, 694
AfiI CCNNNNNNNGG 3 cut(s) 241, 349, 1752
AflIII ACRYGT 3 cut(s) 1582, 1646, 2028
AhlI ACTAGT 1 cut(s) 771
AjiI CACGTC 1 cut(s) 1522
AjnI CCWGG 1 cut(s) 2174
Alw21I GWGCWC 1 cut(s) 1315
Alw26I GTCTC 4 cut(s) 216, 964, 1280, 2566
AlwI GGATC 3 cut(s) 1612, 2366, 2401
AlwNI CAGNNNCTG 1 cut(s) 1927
Ama87I CYCGRG 2 cut(s) 1532, 1599
Aor51HI AGCGCT 2 cut(s) 115, 694
AoxI GGCC 8 cut(s) 701, 780, 974, 1294, 1528, 2172, 2492, 2524
ApeKI GCWGC 7 cut(s) 695, 827, 844, 1033, 1876, 2023, 2284
ApoI RAATTY 5 cut(s) 156, 321, 1051, 1125, 2191
ArsI GACNNNNNNTTYG 6 cut(s) 1324, 1356, 1375, 1407, 1960, 1992
AseI ATTAAT 1 cut(s) 2018
Asp700I GAANNNNTTC 2 cut(s) 140, 1355
Asp718I GGTACC 1 cut(s) 372
AspLEI GCGC 3 cut(s) 116, 695, 1473
AspS9I GGNCC 6 cut(s) 780, 974, 1528, 1821, 2492, 2524
AsuC2I CCSGG 4 cut(s) 1196, 1600, 1601, 1825
AsuHPI GGTGA 2 cut(s) 1162, 1867
AsuII TTCGAA 2 cut(s) 401, 2189
AvaI CYCGRG 2 cut(s) 1532, 1599
AvaII GGWCC 1 cut(s) 1821
BaeI ACNNNNGTAYC 2 cut(s) 2496, 2529
BanI GGYRCC 3 cut(s) 372, 1759, 1861
BanII GRGCYC 3 cut(s) 728, 1315, 1456
BarI GAAGNNNNNNTAC 2 cut(s) 887, 919
BauI CACGAG 3 cut(s) 788, 1778, 2561
BbrPI CACGTG 1 cut(s) 2031
BbsI GAAGAC 3 cut(s) 1151, 1358, 1903
Bbv12I GWGCWC 1 cut(s) 1315
BbvCI CCTCAGC 1 cut(s) 1234
BbvI GCAGC 7 cut(s) 682, 831, 839, 1045, 1888, 2010, 2271
BccI CCATC 7 cut(s) 85, 472, 514, 962, 1852, 2070, 2376
BceAI ACGGC 3 cut(s) 1223, 1353, 1374
BcgI CGANNNNNNTGC 4 cut(s) 391, 425, 1389, 1423
BciT130I CCWGG 1 cut(s) 2176
BciVI GTATCC 2 cut(s) 645, 2497
BclI TGATCA 2 cut(s) 588, 2681
BcnI CCSGG 4 cut(s) 1196, 1600, 1601, 1825
BcoDI GTCTC 4 cut(s) 216, 964, 1280, 2566
BcuI ACTAGT 1 cut(s) 771
BfaI CTAG 8 cut(s) 413, 772, 1184, 1502, 2323, 2612, 2639, 2669
BfmI CTRYAG 1 cut(s) 825
BfoI RGCGCY 2 cut(s) 117, 696
BfuAI ACCTGC 2 cut(s) 360, 402
BfuI GTATCC 2 cut(s) 645, 2497
BglI GCCNNNNNGGC 1 cut(s) 1196
BglII AGATCT 1 cut(s) 167
BisI GCNGC 9 cut(s) 696, 828, 845, 1034, 1188, 1327, 1877, 2024, 2285
BlpI GCTNAGC 1 cut(s) 713
BlsI GCNGC 9 cut(s) 697, 829, 846, 1035, 1189, 1328, 1878, 2025, 2286
Bme1390I CCNGG 5 cut(s) 1196, 1600, 1601, 1825, 2176
Bme18I GGWCC 1 cut(s) 1821
BmeT110I CYCGRG 2 cut(s) 1532, 1599
BmgBI CACGTC 1 cut(s) 1522
BmgT120I GGNCC 6 cut(s) 780, 974, 1528, 1821, 2492, 2524
BmiI GGNNCC 7 cut(s) 314, 374, 1200, 1761, 1863, 1934, 2493
BmrFI CCNGG 5 cut(s) 1196, 1600, 1601, 1825, 2176
BmsI GCATC 7 cut(s) 462, 699, 1452, 2289, 2311, 2452, 2632
BpiI GAAGAC 3 cut(s) 1151, 1358, 1903
BpmI CTGGAG 2 cut(s) 624, 2309
Bpu10I CCTNAGC 2 cut(s) 239, 1234
Bpu1102I GCTNAGC 1 cut(s) 713
Bpu14I TTCGAA 2 cut(s) 401, 2189
BpuEI CTTGAG 1 cut(s) 1659
BpuMI CCSGG 4 cut(s) 1196, 1600, 1601, 1825
BsaAI YACGTR 2 cut(s) 163, 2031
BsaJI CCNNGG 7 cut(s) 1072, 1531, 1599, 1600, 1824, 2175, 2495
BsaXI ACNNNNNCTCC 8 cut(s) 116, 146, 185, 215, 432, 462, 1098, 1128
Bsc4I CCNNNNNNNGG 3 cut(s) 241, 349, 1752
Bse118I RCCGGY 1 cut(s) 1292
Bse1I ACTGG 1 cut(s) 143
Bse3DI GCAATG 4 cut(s) 541, 750, 1405, 2096
BseBI CCWGG 1 cut(s) 2176
BseDI CCNNGG 7 cut(s) 1072, 1531, 1599, 1600, 1824, 2175, 2495
BseGI GGATG 7 cut(s) 96, 105, 264, 529, 950, 1467, 2609
BseLI CCNNNNNNNGG 3 cut(s) 241, 349, 1752
BseMI GCAATG 4 cut(s) 541, 750, 1405, 2096
BseMII CTCAG 6 cut(s) 704, 975, 1112, 1248, 1321, 2020
BseNI ACTGG 1 cut(s) 143
BseRI GAGGAG 1 cut(s) 1796
BseXI GCAGC 7 cut(s) 682, 831, 839, 1045, 1888, 2010, 2271
BseYI CCCAGC 5 cut(s) 971, 1088, 1927, 2251, 2270
BsgI GTGCAG 7 cut(s) 437, 863, 963, 1544, 1755, 2009, 2445
BshFI GGCC 8 cut(s) 703, 782, 976, 1296, 1530, 2174, 2494, 2526
BshNI GGYRCC 3 cut(s) 372, 1759, 1861
BsiHKAI GWGCWC 1 cut(s) 1315
BsiHKCI CYCGRG 2 cut(s) 1532, 1599
BsiSI CCGG 4 cut(s) 1196, 1293, 1600, 1824
BslI CCNNNNNNNGG 3 cut(s) 241, 349, 1752
BsmAI GTCTC 4 cut(s) 216, 964, 1280, 2566
BsmBI CGTCTC 3 cut(s) 964, 1280, 2566
BsmI GAATGC 1 cut(s) 2664
BsnI GGCC 8 cut(s) 703, 782, 976, 1296, 1530, 2174, 2494, 2526
BsoBI CYCGRG 2 cut(s) 1532, 1599
Bsp119I TTCGAA 2 cut(s) 401, 2189
Bsp1286I GDGCHC 3 cut(s) 728, 1315, 1456
Bsp1720I GCTNAGC 1 cut(s) 713
Bsp19I CCATGG 1 cut(s) 2495
BspACI CCGC 8 cut(s) 236, 1188, 1284, 1327, 1459, 1465, 1752, 2386
BspANI GGCC 8 cut(s) 703, 782, 976, 1296, 1530, 2174, 2494, 2526
BspCNI CTCAG 6 cut(s) 705, 974, 1113, 1247, 1320, 2019
BspHI TCATGA 1 cut(s) 1788
BspLI GGNNCC 7 cut(s) 314, 374, 1200, 1761, 1863, 1934, 2493
BspMAI CTGCAG 1 cut(s) 829
BspMI ACCTGC 2 cut(s) 360, 402
BspPI GGATC 3 cut(s) 1612, 2366, 2401
BspQI GCTCTTC 1 cut(s) 683
BspT104I TTCGAA 2 cut(s) 401, 2189
BspT107I GGYRCC 3 cut(s) 372, 1759, 1861
BsrDI GCAATG 4 cut(s) 541, 750, 1405, 2096
BsrFI RCCGGY 1 cut(s) 1292
BsrI ACTGG 1 cut(s) 143
BssAI RCCGGY 1 cut(s) 1292
BssECI CCNNGG 7 cut(s) 1072, 1531, 1599, 1600, 1824, 2175, 2495
BssNAI GTATAC 1 cut(s) 1842
BssSI CACGAG 3 cut(s) 788, 1778, 2561
BssT1I CCWWGG 2 cut(s) 1072, 2495
Bst1107I GTATAC 1 cut(s) 1842
Bst2BI CACGAG 3 cut(s) 788, 1778, 2561
Bst2UI CCWGG 1 cut(s) 2176
Bst4CI ACNGT 8 cut(s) 333, 657, 751, 1110, 1614, 2509, 2520, 2586
Bst6I CTCTTC 4 cut(s) 275, 683, 1172, 2471
BstAPI GCANNNNNTGC 2 cut(s) 211, 2107
BstBAI YACGTR 2 cut(s) 163, 2031
BstBI TTCGAA 2 cut(s) 401, 2189
BstC8I GCNNGC 5 cut(s) 484, 978, 1038, 1079, 1568
BstDEI CTNAG 7 cut(s) 239, 713, 961, 1121, 1234, 1307, 2006
BstDSI CCRYGG 1 cut(s) 2495
BstF5I GGATG 7 cut(s) 96, 105, 264, 529, 950, 1467, 2609
BstH2I RGCGCY 2 cut(s) 117, 696
BstHHI GCGC 3 cut(s) 116, 695, 1473
BstMAI GTCTC 4 cut(s) 216, 964, 1280, 2566
BstNI CCWGG 1 cut(s) 2176
BstNSI RCATGY 3 cut(s) 1586, 1650, 2545
BstSCI CCNGG 5 cut(s) 1194, 1598, 1599, 1823, 2174
BstSFI CTRYAG 1 cut(s) 825
BstSNI TACGTA 1 cut(s) 163
BstV1I GCAGC 7 cut(s) 682, 831, 839, 1045, 1888, 2010, 2271
BstV2I GAAGAC 3 cut(s) 1151, 1358, 1903
BstX2I RGATCY 2 cut(s) 167, 2406
BstXI CCANNNNNNTGG 1 cut(s) 2287
BstYI RGATCY 2 cut(s) 167, 2406
BstZ17I GTATAC 1 cut(s) 1842
BsuI GTATCC 2 cut(s) 645, 2497
BsuRI GGCC 8 cut(s) 703, 782, 976, 1296, 1530, 2174, 2494, 2526
BtgI CCRYGG 1 cut(s) 2495
BtrI CACGTC 1 cut(s) 1522
BtsCI GGATG 7 cut(s) 96, 105, 264, 529, 950, 1467, 2609
BtsI GCAGTG 4 cut(s) 490, 724, 822, 1988
BtsIMutI CAGTG 9 cut(s) 329, 490, 724, 822, 1106, 1988, 2094, 2514, 2591
BveI ACCTGC 2 cut(s) 360, 402
Cac8I GCNNGC 5 cut(s) 484, 978, 1038, 1079, 1568
CaiI CAGNNNCTG 1 cut(s) 1927
CciI TCATGA 1 cut(s) 1788
CfoI GCGC 3 cut(s) 116, 695, 1473
Cfr10I RCCGGY 1 cut(s) 1292
Cfr13I GGNCC 6 cut(s) 780, 974, 1528, 1821, 2492, 2524
Cfr9I CCCGGG 1 cut(s) 1599
CseI GACGC 2 cut(s) 2194, 2583
Csp6I GTAC 7 cut(s) 296, 373, 391, 626, 937, 965, 1510
CviQI GTAC 7 cut(s) 296, 373, 391, 626, 937, 965, 1510
DdeI CTNAG 7 cut(s) 239, 713, 961, 1121, 1234, 1307, 2006
DrdI GACNNNNNNGTC 1 cut(s) 1342
DseDI GACNNNNNNGTC 1 cut(s) 1342
Eam1104I CTCTTC 4 cut(s) 275, 683, 1172, 2471
EarI CTCTTC 4 cut(s) 275, 683, 1172, 2471
Ecl136II GAGCTC 1 cut(s) 1313
Eco105I TACGTA 1 cut(s) 163
Eco130I CCWWGG 2 cut(s) 1072, 2495
Eco147I AGGCCT 1 cut(s) 703
Eco24I GRGCYC 3 cut(s) 728, 1315, 1456
Eco32I GATATC 1 cut(s) 1743
Eco47I GGWCC 1 cut(s) 1821
Eco47III AGCGCT 2 cut(s) 115, 694
Eco53kI GAGCTC 1 cut(s) 1313
Eco57I CTGAAG 1 cut(s) 716
Eco72I CACGTG 1 cut(s) 2031
Eco88I CYCGRG 2 cut(s) 1532, 1599
EcoICRI GAGCTC 1 cut(s) 1313
EcoO109I RGGNCCY 1 cut(s) 1528
EcoRII CCWGG 1 cut(s) 2174
EcoRV GATATC 1 cut(s) 1743
EcoT14I CCWWGG 2 cut(s) 1072, 2495
EcoT22I ATGCAT 3 cut(s) 619, 2304, 2445
EcoT38I GRGCYC 3 cut(s) 728, 1315, 1456
ErhI CCWWGG 2 cut(s) 1072, 2495
Esp3I CGTCTC 3 cut(s) 964, 1280, 2566
FauI CCCGC 3 cut(s) 243, 1291, 1745
FbaI TGATCA 2 cut(s) 588, 2681
FblI GTMKAC 1 cut(s) 1841
Fnu4HI GCNGC 9 cut(s) 696, 828, 845, 1034, 1188, 1327, 1877, 2024, 2285
FokI GGATG 7 cut(s) 103, 112, 251, 536, 937, 1474, 2616
FriOI GRGCYC 3 cut(s) 728, 1315, 1456
Fsp4HI GCNGC 9 cut(s) 696, 828, 845, 1034, 1188, 1327, 1877, 2024, 2285
FspBI CTAG 8 cut(s) 413, 772, 1184, 1502, 2323, 2612, 2639, 2669
FspI TGCGCA 1 cut(s) 1472
GlaI GCGC 3 cut(s) 115, 694, 1472
GluI GCNGC 9 cut(s) 696, 828, 845, 1034, 1188, 1327, 1877, 2024, 2285
GsaI CCCAGC 5 cut(s) 975, 1092, 1931, 2255, 2274
GsuI CTGGAG 2 cut(s) 624, 2309
HaeII RGCGCY 2 cut(s) 117, 696
HaeIII GGCC 8 cut(s) 703, 782, 976, 1296, 1530, 2174, 2494, 2526
HapII CCGG 4 cut(s) 1196, 1293, 1600, 1824
HgaI GACGC 2 cut(s) 2194, 2583
HhaI GCGC 3 cut(s) 116, 695, 1473
Hin6I GCGC 3 cut(s) 114, 693, 1471
HinP1I GCGC 3 cut(s) 114, 693, 1471
HincII GTYRAC 5 cut(s) 1045, 1721, 2342, 2532, 2594
HindII GTYRAC 5 cut(s) 1045, 1721, 2342, 2532, 2594
HindIII AAGCTT 2 cut(s) 1211, 1421
HpaI GTTAAC 2 cut(s) 2342, 2532
HpaII CCGG 4 cut(s) 1196, 1293, 1600, 1824
HphI GGTGA 2 cut(s) 1162, 1867
Hpy166II GTNNAC 8 cut(s) 64, 634, 1045, 1721, 1842, 2342, 2532, 2594
Hpy8I GTNNAC 8 cut(s) 64, 634, 1045, 1721, 1842, 2342, 2532, 2594
Hpy99I CGWCG 3 cut(s) 1339, 1523, 2631
HpyAV CCTTC 8 cut(s) 408, 733, 757, 883, 1187, 1272, 2164, 2239
HpyCH4III ACNGT 8 cut(s) 333, 657, 751, 1110, 1614, 2509, 2520, 2586
HpyCH4IV ACGT 8 cut(s) 162, 558, 578, 982, 1355, 1521, 1905, 2030
HpyF3I CTNAG 7 cut(s) 239, 713, 961, 1121, 1234, 1307, 2006
HpySE526I ACGT 8 cut(s) 162, 558, 578, 982, 1355, 1521, 1905, 2030
HspAI GCGC 3 cut(s) 114, 693, 1471
KpnI GGTACC 1 cut(s) 376
Ksp22I TGATCA 2 cut(s) 588, 2681
KspAI GTTAAC 2 cut(s) 2342, 2532
LguI GCTCTTC 1 cut(s) 683
LmnI GCTCC 7 cut(s) 10, 38, 312, 1204, 1229, 1873, 2248
Lsp1109I GCAGC 7 cut(s) 682, 831, 839, 1045, 1888, 2010, 2271
LweI GCATC 7 cut(s) 462, 699, 1452, 2289, 2311, 2452, 2632
MaeI CTAG 8 cut(s) 413, 772, 1184, 1502, 2323, 2612, 2639, 2669
MaeII ACGT 8 cut(s) 162, 558, 578, 982, 1355, 1521, 1905, 2030
MaeIII GTNAC 7 cut(s) 361, 751, 774, 1110, 1228, 1578, 2565
MfeI CAATTG 2 cut(s) 215, 1943
MflI RGATCY 2 cut(s) 167, 2406
MhlI GDGCHC 3 cut(s) 728, 1315, 1456
MlyI GAGTC 2 cut(s) 78, 1061
MmeI TCCRAC 3 cut(s) 171, 1535, 2434
Mph1103I ATGCAT 3 cut(s) 619, 2304, 2445
MroXI GAANNNNTTC 2 cut(s) 140, 1355
MseI TTAA 9 cut(s) 26, 497, 1055, 1714, 2018, 2210, 2341, 2354, 2531
MslI CAYNNNNRTG 2 cut(s) 59, 269
MspA1I CMGCKG 1 cut(s) 1092
MspI CCGG 4 cut(s) 1196, 1293, 1600, 1824
MspR9I CCNGG 5 cut(s) 1196, 1600, 1601, 1825, 2176
MunI CAATTG 2 cut(s) 215, 1943
Mva1269I GAATGC 1 cut(s) 2664
MvaI CCWGG 1 cut(s) 2176
NciI CCSGG 4 cut(s) 1196, 1600, 1601, 1825
NcoI CCATGG 1 cut(s) 2495
NlaIV GGNNCC 7 cut(s) 314, 374, 1200, 1761, 1863, 1934, 2493
NmuCI GTSAC 4 cut(s) 751, 774, 1578, 2565
NsbI TGCGCA 1 cut(s) 1472
NsiI ATGCAT 3 cut(s) 619, 2304, 2445
NspI RCATGY 3 cut(s) 1586, 1650, 2545
NspV TTCGAA 2 cut(s) 401, 2189
PagI TCATGA 1 cut(s) 1788
PceI AGGCCT 1 cut(s) 703
PciI ACATGT 2 cut(s) 1582, 1646
PciSI GCTCTTC 1 cut(s) 683
PcsI WCGNNNNNNNCGW 1 cut(s) 1340
PctI GAATGC 1 cut(s) 2664
PdmI GAANNNNTTC 2 cut(s) 140, 1355
PkrI GCNGC 9 cut(s) 697, 829, 846, 1035, 1189, 1328, 1878, 2025, 2286
PleI GAGTC 2 cut(s) 78, 1061
PmaCI CACGTG 1 cut(s) 2031
PmlI CACGTG 1 cut(s) 2031
PpsI GAGTC 2 cut(s) 78, 1061
Ppu21I YACGTR 2 cut(s) 163, 2031
PscI ACATGT 2 cut(s) 1582, 1646
PshBI ATTAAT 1 cut(s) 2018
Psp124BI GAGCTC 1 cut(s) 1315
Psp6I CCWGG 1 cut(s) 2174
PspCI CACGTG 1 cut(s) 2031
PspFI CCCAGC 5 cut(s) 971, 1088, 1927, 2251, 2270
PspGI CCWGG 1 cut(s) 2174
PspN4I GGNNCC 7 cut(s) 314, 374, 1200, 1761, 1863, 1934, 2493
PspPI GGNCC 6 cut(s) 780, 974, 1528, 1821, 2492, 2524
PstI CTGCAG 1 cut(s) 829
PstNI CAGNNNCTG 1 cut(s) 1927
PsuI RGATCY 2 cut(s) 167, 2406
PvuII CAGCTG 1 cut(s) 1092
RsaI GTAC 7 cut(s) 297, 374, 392, 627, 938, 966, 1511
RsaNI GTAC 7 cut(s) 296, 373, 391, 626, 937, 965, 1510
RseI CAYNNNNRTG 2 cut(s) 59, 269
SacI GAGCTC 1 cut(s) 1315
SapI GCTCTTC 1 cut(s) 683
SaqAI TTAA 9 cut(s) 26, 497, 1055, 1714, 2018, 2210, 2341, 2354, 2531
SatI GCNGC 9 cut(s) 696, 828, 845, 1034, 1188, 1327, 1877, 2024, 2285
Sau96I GGNCC 6 cut(s) 780, 974, 1528, 1821, 2492, 2524
SchI GAGTC 2 cut(s) 78, 1061
ScrFI CCNGG 5 cut(s) 1196, 1600, 1601, 1825, 2176
SduI GDGCHC 3 cut(s) 728, 1315, 1456
SfaNI GCATC 7 cut(s) 462, 699, 1452, 2289, 2311, 2452, 2632
SfcI CTRYAG 1 cut(s) 825
SfuI TTCGAA 2 cut(s) 401, 2189
SinI GGWCC 1 cut(s) 1821
SmaI CCCGGG 1 cut(s) 1601
SmiMI CAYNNNNRTG 2 cut(s) 59, 269
SmlI CTYRAG 1 cut(s) 1638
SmoI CTYRAG 1 cut(s) 1638
SnaBI TACGTA 1 cut(s) 163
SpeI ACTAGT 1 cut(s) 771
SseBI AGGCCT 1 cut(s) 703
SsiI CCGC 8 cut(s) 236, 1188, 1284, 1327, 1459, 1465, 1752, 2386
SspMI CTAG 8 cut(s) 413, 772, 1184, 1502, 2323, 2612, 2639, 2669
SstI GAGCTC 1 cut(s) 1315
StuI AGGCCT 1 cut(s) 703
StyD4I CCNGG 5 cut(s) 1194, 1598, 1599, 1823, 2174
StyI CCWWGG 2 cut(s) 1072, 2495
TaaI ACNGT 8 cut(s) 333, 657, 751, 1110, 1614, 2509, 2520, 2586
TaiI ACGT 8 cut(s) 165, 561, 581, 985, 1358, 1524, 1908, 2033
TatI WGTACW 1 cut(s) 964
TauI GCSGC 2 cut(s) 1190, 1329
Tru1I TTAA 9 cut(s) 26, 497, 1055, 1714, 2018, 2210, 2341, 2354, 2531
Tru9I TTAA 9 cut(s) 26, 497, 1055, 1714, 2018, 2210, 2341, 2354, 2531
TscAI CASTG 9 cut(s) 336, 497, 724, 829, 1113, 1995, 2094, 2514, 2591
TseFI GTSAC 4 cut(s) 751, 774, 1578, 2565
TseI GCWGC 7 cut(s) 695, 827, 844, 1033, 1876, 2023, 2284
Tsp45I GTSAC 4 cut(s) 751, 774, 1578, 2565
TspDTI ATGAA 8 cut(s) 43, 315, 334, 522, 701, 2085, 2410, 2476
TspGWI ACGGA 4 cut(s) 81, 849, 1216, 1262
TspMI CCCGGG 1 cut(s) 1599
TspRI CASTG 9 cut(s) 336, 497, 724, 829, 1113, 1995, 2094, 2514, 2591
VpaK11BI GGWCC 1 cut(s) 1821
VspI ATTAAT 1 cut(s) 2018
XapI RAATTY 5 cut(s) 156, 321, 1051, 1125, 2191
XbaI TCTAGA 1 cut(s) 2668
XceI RCATGY 3 cut(s) 1586, 1650, 2545
XcmI CCANNNNNNNNNTGG 1 cut(s) 1753
XmaI CCCGGG 1 cut(s) 1599
XmiI GTMKAC 1 cut(s) 1841
XmnI GAANNNNTTC 2 cut(s) 140, 1355
XspI CTAG 8 cut(s) 413, 772, 1184, 1502, 2323, 2612, 2639, 2669
Zsp2I ATGCAT 3 cut(s) 619, 2304, 2445
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.