Rh1DG383000

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0008946
Physical Location & Seq
Reverse (-)
60386062 .. 60389837
3776 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG383000.1

Sequence Viewer

Length: 1053 bp
ATGGAATCCACCATTTCTTTATCGTCTTCCTCTCTTCTCCTACCTTCGCCTCGCCGCCGACTCGGCTCCAAATCCCAAGCTTCAATTTCGCTCCGTAACCTCCGCCGTCCGAGCCTCAAATCCAGAACCTTCCTCGTTCCGTCTCTTTCCCGCCAAAACCGGCCTATCCGATCTCAGAGCTCGAATCATCAGCCGCAGGACGACGGCTTCGTCCTCGAAGACGTTCCTCACTTGACCGATTTCCTCCCAAACTTGCCGTCATATCCGAATCCATTGCAACAGAGCCAAGCTTATGCAGTTGTCAAGAAAACTTTTGTGAGCCCTGCGGATGCGGTTGCGCAGAAAGTTGTTGTTCAAGATGATAGTCCTAGAGGGGTACATTTTCGACGTGCAGGGCCTCGGGAAAAGGTTTATTTCAAGTCGGAGGAAGTGCGTGCTTGTATAGTGACATGTGGGGGTTTATGCCCGGGGATCAATACTGTGATTCGAGAGATAGTATGTGGCTTGAGCTACATGTATGGTGTTGAGGATATACTTGGAATTGAGGGAGGATATAGAGGCTTTTATTCTAAAAATACATTAAAGTTGACACCTAAAGTTGTCAATGATATCCATAAGCGGGGTGGCACCTTTCTCCGCACTTCACGAGGAGGTCATGATACCAACAAGATAGTTGATAACATACAGGACCGGGGAATAAATCAGGTATACATAATTGGAGGTGATGGCACCCAAAAAGGAGCAGCTCTAATATATAAGGAAGTTGAGAAACGTGGTCTTCAAGTGGCAGTTGCTGGGGTTCCTAAAACAATTGACAATGATATTGCTGTGATAGACAAGTCTTTTGGTTTTGACACTGCTGTTGAAGAAGCTCAGAGGGCTATTAATGCTGCACACGTGGAGGTTGAAAGTGTGGAAAATGGAATCGGAATTGTCAAACTGATGGGAAGATACAGTGGCTTCATTGCATTATATGCAACTTTGGCAAGTCGAGATGTGGATCATTTTACAAAAGGTCGGCAGATGGCGGGTGATTTCAGCTCTGGTTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003872 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008443 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009507 GO:0009536 GO:0009987 GO:0016052 GO:0016053 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019200 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044262 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046835 GO:0046939 GO:0051186 GO:0051188 GO:0055086 GO:0061615 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00030 ko00051 ko00052 ko00680 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko03018 ko04152 ko05230 map00010 map00030 map00051 map00052 map00680 map01100 map01110 map01120 map01130 map01200 map01230 map03018 map04152 map05230
Pfam Domains
Protein Families
PFK

Protein Analysis

350

Amino Acids

38.28

Weight (kDa)

9.4

Isoelectric Point (pI)

52.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PFK PF00365 146 - 330 5.6e-49 Phosphofructokinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 209
Acc16I TGCGCA 1 cut(s) 339
AccB1I GGYRCC 2 cut(s) 626, 728
AccI GTMKAC 1 cut(s) 708
AciI CCGC 9 cut(s) 55, 103, 151, 194, 326, 332, 619, 637, 1028
AclWI GGATC 2 cut(s) 479, 1008
AcvI CACGTG 1 cut(s) 898
AfaI GTAC 1 cut(s) 378
AfiI CCNNNNNNNGG 1 cut(s) 619
AflIII ACRYGT 3 cut(s) 449, 513, 895
AgsI TTSAA 6 cut(s) 84, 356, 418, 782, 866, 908
AjiI CACGTC 1 cut(s) 389
AluBI AGCT 7 cut(s) 80, 180, 290, 510, 746, 872, 1041
AluI AGCT 7 cut(s) 80, 180, 290, 510, 746, 872, 1041
Alw21I GWGCWC 1 cut(s) 182
Alw26I GTCTC 1 cut(s) 147
AlwI GGATC 2 cut(s) 479, 1008
AlwNI CAGNNNCTG 1 cut(s) 794
Ama87I CYCGRG 2 cut(s) 399, 466
AoxI GGCC 2 cut(s) 161, 395
ApeKI GCWGC 2 cut(s) 743, 890
ArsI GACNNNNNNTTYG 6 cut(s) 191, 223, 242, 274, 827, 859
AseI ATTAAT 1 cut(s) 885
Asp700I GAANNNNTTC 1 cut(s) 222
AspLEI GCGC 1 cut(s) 340
AspS9I GGNCC 2 cut(s) 395, 688
AsuC2I CCSGG 3 cut(s) 467, 468, 692
AsuHPI GGTGA 2 cut(s) 734, 1043
AvaI CYCGRG 2 cut(s) 399, 466
AvaII GGWCC 1 cut(s) 688
BanI GGYRCC 2 cut(s) 626, 728
BanII GRGCYC 2 cut(s) 182, 323
BauI CACGAG 1 cut(s) 645
BbrPI CACGTG 1 cut(s) 898
BbsI GAAGAC 3 cut(s) 18, 225, 770
Bbv12I GWGCWC 1 cut(s) 182
BbvI GCAGC 2 cut(s) 755, 877
BccI CCATC 3 cut(s) 719, 937, 1018
BceAI ACGGC 3 cut(s) 90, 220, 241
BcgI CGANNNNNNTGC 2 cut(s) 256, 290
BcnI CCSGG 3 cut(s) 467, 468, 692
BcoDI GTCTC 1 cut(s) 147
BfaI CTAG 1 cut(s) 369
BglI GCCNNNNNGGC 1 cut(s) 63
BisI GCNGC 4 cut(s) 55, 194, 744, 891
BlsI GCNGC 4 cut(s) 56, 195, 745, 892
Bme1390I CCNGG 3 cut(s) 467, 468, 692
Bme18I GGWCC 1 cut(s) 688
BmeT110I CYCGRG 2 cut(s) 399, 466
BmgBI CACGTC 1 cut(s) 389
BmgT120I GGNCC 2 cut(s) 395, 688
BmiI GGNNCC 4 cut(s) 67, 628, 730, 801
BmrFI CCNGG 3 cut(s) 467, 468, 692
BmsI GCATC 1 cut(s) 319
BpiI GAAGAC 3 cut(s) 18, 225, 770
BpuEI CTTGAG 1 cut(s) 526
BpuMI CCSGG 3 cut(s) 467, 468, 692
BsaAI YACGTR 1 cut(s) 898
BsaBI GATNNNNATC 1 cut(s) 999
BsaJI CCNNGG 4 cut(s) 398, 466, 467, 691
Bsc4I CCNNNNNNNGG 1 cut(s) 619
Bse118I RCCGGY 1 cut(s) 159
Bse3DI GCAATG 2 cut(s) 272, 963
Bse8I GATNNNNATC 1 cut(s) 999
BseDI CCNNGG 4 cut(s) 398, 466, 467, 691
BseGI GGATG 1 cut(s) 334
BseJI GATNNNNATC 1 cut(s) 999
BseLI CCNNNNNNNGG 1 cut(s) 619
BseMI GCAATG 2 cut(s) 272, 963
BseMII CTCAG 2 cut(s) 188, 887
BseRI GAGGAG 1 cut(s) 663
BseXI GCAGC 2 cut(s) 755, 877
BseYI CCCAGC 1 cut(s) 794
BsgI GTGCAG 2 cut(s) 411, 876
BshFI GGCC 2 cut(s) 163, 397
BshNI GGYRCC 2 cut(s) 626, 728
BsiHKAI GWGCWC 1 cut(s) 182
BsiHKCI CYCGRG 2 cut(s) 399, 466
BsiSI CCGG 3 cut(s) 160, 467, 691
BslI CCNNNNNNNGG 1 cut(s) 619
BsmAI GTCTC 1 cut(s) 147
BsmBI CGTCTC 1 cut(s) 147
BsnI GGCC 2 cut(s) 163, 397
BsoBI CYCGRG 2 cut(s) 399, 466
Bsp1286I GDGCHC 2 cut(s) 182, 323
Bsp143I GATC 3 cut(s) 170, 471, 1000
BspACI CCGC 9 cut(s) 55, 103, 151, 194, 326, 332, 619, 637, 1028
BspANI GGCC 2 cut(s) 163, 397
BspCNI CTCAG 2 cut(s) 187, 886
BspHI TCATGA 1 cut(s) 655
BspLI GGNNCC 4 cut(s) 67, 628, 730, 801
BspPI GGATC 2 cut(s) 479, 1008
BspT107I GGYRCC 2 cut(s) 626, 728
BsrDI GCAATG 2 cut(s) 272, 963
BsrFI RCCGGY 1 cut(s) 159
BssAI RCCGGY 1 cut(s) 159
BssECI CCNNGG 4 cut(s) 398, 466, 467, 691
BssMI GATC 3 cut(s) 170, 471, 1000
BssNAI GTATAC 1 cut(s) 709
BssSI CACGAG 1 cut(s) 645
Bst1107I GTATAC 1 cut(s) 709
Bst2BI CACGAG 1 cut(s) 645
Bst4CI ACNGT 2 cut(s) 481, 956
Bst6I CTCTTC 1 cut(s) 39
BstAPI GCANNNNNTGC 1 cut(s) 974
BstBAI YACGTR 1 cut(s) 898
BstC8I GCNNGC 1 cut(s) 435
BstDEI CTNAG 2 cut(s) 174, 873
BstF5I GGATG 1 cut(s) 334
BstHHI GCGC 1 cut(s) 340
BstKTI GATC 3 cut(s) 173, 474, 1003
BstMAI GTCTC 1 cut(s) 147
BstMBI GATC 3 cut(s) 170, 471, 1000
BstMWI GCNNNNNNNGC 6 cut(s) 63, 111, 878, 887, 974, 983
BstNSI RCATGY 2 cut(s) 453, 517
BstSCI CCNGG 3 cut(s) 465, 466, 690
BstV1I GCAGC 2 cut(s) 755, 877
BstV2I GAAGAC 3 cut(s) 18, 225, 770
BstZ17I GTATAC 1 cut(s) 709
BsuRI GGCC 2 cut(s) 163, 397
BtrI CACGTC 1 cut(s) 389
BtsCI GGATG 1 cut(s) 334
BtsI GCAGTG 1 cut(s) 855
BtsIMutI CAGTG 2 cut(s) 855, 961
Cac8I GCNNGC 1 cut(s) 435
CaiI CAGNNNCTG 1 cut(s) 794
CciI TCATGA 1 cut(s) 655
CfoI GCGC 1 cut(s) 340
Cfr10I RCCGGY 1 cut(s) 159
Cfr13I GGNCC 2 cut(s) 395, 688
Cfr9I CCCGGG 1 cut(s) 466
Csp6I GTAC 1 cut(s) 377
CviAII CATG 3 cut(s) 450, 514, 656
CviQI GTAC 1 cut(s) 377
DdeI CTNAG 2 cut(s) 174, 873
DpnI GATC 3 cut(s) 172, 473, 1002
DpnII GATC 3 cut(s) 170, 471, 1000
DrdI GACNNNNNNGTC 1 cut(s) 209
DseDI GACNNNNNNGTC 1 cut(s) 209
Eam1104I CTCTTC 1 cut(s) 39
EarI CTCTTC 1 cut(s) 39
EciI GGCGGA 1 cut(s) 92
Ecl136II GAGCTC 1 cut(s) 180
Eco24I GRGCYC 2 cut(s) 182, 323
Eco32I GATATC 1 cut(s) 610
Eco47I GGWCC 1 cut(s) 688
Eco53kI GAGCTC 1 cut(s) 180
Eco72I CACGTG 1 cut(s) 898
Eco88I CYCGRG 2 cut(s) 399, 466
EcoICRI GAGCTC 1 cut(s) 180
EcoO109I RGGNCCY 1 cut(s) 395
EcoRV GATATC 1 cut(s) 610
EcoT38I GRGCYC 2 cut(s) 182, 323
Esp3I CGTCTC 1 cut(s) 147
FaeI CATG 3 cut(s) 453, 517, 659
FatI CATG 3 cut(s) 449, 513, 655
FauI CCCGC 3 cut(s) 158, 612, 1021
FblI GTMKAC 1 cut(s) 708
Fnu4HI GCNGC 4 cut(s) 55, 194, 744, 891
FokI GGATG 1 cut(s) 341
FriOI GRGCYC 2 cut(s) 182, 323
Fsp4HI GCNGC 4 cut(s) 55, 194, 744, 891
FspBI CTAG 1 cut(s) 369
FspI TGCGCA 1 cut(s) 339
GlaI GCGC 1 cut(s) 339
GluI GCNGC 4 cut(s) 55, 194, 744, 891
GsaI CCCAGC 1 cut(s) 798
HaeIII GGCC 2 cut(s) 163, 397
HapII CCGG 3 cut(s) 160, 467, 691
HhaI GCGC 1 cut(s) 340
Hin1II CATG 3 cut(s) 453, 517, 659
Hin6I GCGC 1 cut(s) 338
HinP1I GCGC 1 cut(s) 338
HincII GTYRAC 1 cut(s) 588
HindII GTYRAC 1 cut(s) 588
HindIII AAGCTT 2 cut(s) 78, 288
HinfI GANTC 6 cut(s) 5, 60, 184, 268, 484, 924
HpaII CCGG 3 cut(s) 160, 467, 691
HphI GGTGA 2 cut(s) 734, 1043
Hpy166II GTNNAC 2 cut(s) 588, 709
Hpy188I TCNGA 7 cut(s) 111, 170, 177, 267, 424, 876, 929
Hpy188III TCNNGA 8 cut(s) 123, 304, 356, 401, 488, 645, 656, 992
Hpy8I GTNNAC 2 cut(s) 588, 709
Hpy99I CGWCG 2 cut(s) 206, 390
HpyAV CCTTC 2 cut(s) 54, 139
HpyCH4III ACNGT 2 cut(s) 481, 956
HpyCH4IV ACGT 4 cut(s) 222, 388, 772, 897
HpyCH4V TGCA 6 cut(s) 277, 296, 392, 893, 968, 977
HpyF10VI GCNNNNNNNGC 6 cut(s) 63, 111, 878, 887, 974, 983
HpyF3I CTNAG 2 cut(s) 174, 873
HpySE526I ACGT 4 cut(s) 222, 388, 772, 897
Hsp92II CATG 3 cut(s) 453, 517, 659
HspAI GCGC 1 cut(s) 338
Kzo9I GATC 3 cut(s) 170, 471, 1000
LmnI GCTCC 3 cut(s) 71, 96, 740
Lsp1109I GCAGC 2 cut(s) 755, 877
LweI GCATC 1 cut(s) 319
MaeI CTAG 1 cut(s) 369
MaeII ACGT 4 cut(s) 222, 388, 772, 897
MaeIII GTNAC 2 cut(s) 95, 445
MalI GATC 3 cut(s) 172, 473, 1002
MboI GATC 3 cut(s) 170, 471, 1000
MboII GAAGA 6 cut(s) 18, 26, 230, 770, 878, 960
MfeI CAATTG 1 cut(s) 810
MhlI GDGCHC 2 cut(s) 182, 323
MluCI AATT 5 cut(s) 84, 540, 714, 810, 930
MlyI GAGTC 1 cut(s) 54
MmeI TCCRAC 1 cut(s) 402
MroXI GAANNNNTTC 1 cut(s) 222
MseI TTAA 2 cut(s) 581, 885
MspI CCGG 3 cut(s) 160, 467, 691
MspR9I CCNGG 3 cut(s) 467, 468, 692
MunI CAATTG 1 cut(s) 810
MwoI GCNNNNNNNGC 6 cut(s) 63, 111, 878, 887, 974, 983
NciI CCSGG 3 cut(s) 467, 468, 692
NdeII GATC 3 cut(s) 170, 471, 1000
NlaIII CATG 3 cut(s) 453, 517, 659
NlaIV GGNNCC 4 cut(s) 67, 628, 730, 801
NmeAIII GCCGAG 1 cut(s) 42
NmuCI GTSAC 1 cut(s) 445
NsbI TGCGCA 1 cut(s) 339
NspI RCATGY 2 cut(s) 453, 517
PagI TCATGA 1 cut(s) 655
PciI ACATGT 2 cut(s) 449, 513
PcsI WCGNNNNNNNCGW 1 cut(s) 207
PdmI GAANNNNTTC 1 cut(s) 222
PfeI GAWTC 5 cut(s) 5, 184, 268, 484, 924
PkrI GCNGC 4 cut(s) 56, 195, 745, 892
PleI GAGTC 1 cut(s) 54
PmaCI CACGTG 1 cut(s) 898
PmlI CACGTG 1 cut(s) 898
PpsI GAGTC 1 cut(s) 54
Ppu21I YACGTR 1 cut(s) 898
PscI ACATGT 2 cut(s) 449, 513
PshBI ATTAAT 1 cut(s) 885
Psp124BI GAGCTC 1 cut(s) 182
PspCI CACGTG 1 cut(s) 898
PspFI CCCAGC 1 cut(s) 794
PspN4I GGNNCC 4 cut(s) 67, 628, 730, 801
PspPI GGNCC 2 cut(s) 395, 688
PstNI CAGNNNCTG 1 cut(s) 794
RsaI GTAC 1 cut(s) 378
RsaNI GTAC 1 cut(s) 377
SacI GAGCTC 1 cut(s) 182
SaqAI TTAA 2 cut(s) 581, 885
SatI GCNGC 4 cut(s) 55, 194, 744, 891
Sau3AI GATC 3 cut(s) 170, 471, 1000
Sau96I GGNCC 2 cut(s) 395, 688
SchI GAGTC 1 cut(s) 54
ScrFI CCNGG 3 cut(s) 467, 468, 692
SduI GDGCHC 2 cut(s) 182, 323
SfaNI GCATC 1 cut(s) 319
SinI GGWCC 1 cut(s) 688
SmaI CCCGGG 1 cut(s) 468
SmlI CTYRAG 1 cut(s) 505
SmoI CTYRAG 1 cut(s) 505
Sse9I AATT 5 cut(s) 84, 540, 714, 810, 930
SsiI CCGC 9 cut(s) 55, 103, 151, 194, 326, 332, 619, 637, 1028
SspMI CTAG 1 cut(s) 369
SstI GAGCTC 1 cut(s) 182
StyD4I CCNGG 3 cut(s) 465, 466, 690
TaaI ACNGT 2 cut(s) 481, 956
TaiI ACGT 4 cut(s) 225, 391, 775, 900
TaqI TCGA 5 cut(s) 182, 216, 385, 487, 991
TaqII GACCGA 1 cut(s) 251
TasI AATT 5 cut(s) 84, 540, 714, 810, 930
TauI GCSGC 2 cut(s) 57, 196
TfiI GAWTC 5 cut(s) 5, 184, 268, 484, 924
Tru1I TTAA 2 cut(s) 581, 885
Tru9I TTAA 2 cut(s) 581, 885
TscAI CASTG 2 cut(s) 862, 961
TseFI GTSAC 1 cut(s) 445
TseI GCWGC 2 cut(s) 743, 890
Tsp45I GTSAC 1 cut(s) 445
TspDTI ATGAA 1 cut(s) 952
TspGWI ACGGA 2 cut(s) 83, 129
TspMI CCCGGG 1 cut(s) 466
TspRI CASTG 2 cut(s) 862, 961
VpaK11BI GGWCC 1 cut(s) 688
VspI ATTAAT 1 cut(s) 885
XceI RCATGY 2 cut(s) 453, 517
XcmI CCANNNNNNNNNTGG 1 cut(s) 620
XmaI CCCGGG 1 cut(s) 466
XmiI GTMKAC 1 cut(s) 708
XmnI GAANNNNTTC 1 cut(s) 222
XspI CTAG 1 cut(s) 369
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.