MD01G1177300.v1.1

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0008946
Physical Location & Seq
Reverse (-)
27800492 .. 27805591
5100 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1177300.v1.1.491

Sequence Viewer

Length: 1623 bp
ATGGAATCCACCATTTCATCTCCTATCTCCTCCACCAAGTCCTCGCCCCTGTTGCATTCGGCTTCCCATTCACTCGGCTCCAGATCCCAGTCTTTTCCGTCGCTCCCGTCGCTTCCGTCGGTTCAAACCCTCCGCTATCCCAGCTTCAAATCCAGAAGCTTCCTCGGATTTCCCTTCCGTTCGCGCCAAAACCAGCCTATCCGATCTCAGAGCTCGAATCATCAGCCGGCTAACGACGGCTTCGTCCTCGAAGACGTTCCTCACTTGACTAATTTTCTCCCTAATTTGCCGTCATATCCAAATCCATTGAAACAGAGCCAAGCTTATGCAGTTGTCAAGCAAACTTTTGTGAGCCCTCACGATGTGGTTGCGCAGAAAGTTGTTGTTCAAAAGGAGGGTCCTAGAGGGGTGCATTTCCGACGTGCAGGACCCCGGGAAAAGGTTTATTTCAAGTCGGATGAAGTTCGTGCTTGCATTGTCACGTGTGGGGGTTTATGCCCGGGGATCAATACTGTGATTCGGGAAATAGTATGCGGCTTGAACTACATGTATGGTGTTGAAGATATACTTGGAATTGAGGGAGGATATAGGGGATTTTACTCTAAAAATACGTTGAGGTTGACACCAAAAGTTGTCAATGATATCCATAAGCGCGGTGGCACCTTTCTTCGAACTTCACGAGGAGGTCATGATACAAACAAGATAGTTGATAACATACAGGACCGAGGAATAAATCAGGTATACATAATTGGAGGCGATGGCACTCAAAGAGGAGCAGATCTTATATACAAGGAAGTTGAGAAACGTGGTCTTCAAGTGGCAGTTGCTGGGATCCCTAAGACAATTGATAATGATATTGCTGTGATAGACAAATCTTTTGGTTTCGATACTGCTGTTGAAGAAGCACAGAGAGCTATTAACGCCGCACATGTGGAGGCTGAAAGTGTGGACAATGGAGTTGGAATTGTCAAACTGATGGGCAGATACAGTGGTTTCATTGCTCTAAATGCAACTTTGGCAAGTCGAGATGTGGATTGCTGCTTGATTCCAGAATCTCCTTTCTATTTGGAAGGCCGGGGTGGGCTTTTTGAATTTGTCGAAGAGCGGCTTAAAGAAAATGGACATGTGGTTATTGTGTTAGCAGAAGGAGCAGGGCAGGAATATATTTCAGGGGAGATGAATGCAGTTGATGTGAAAGATGCATCAGGAAATAAGCTACTCCTCGATGTTGGTCTATGGTTAACCCAAAAGATTAAGGATCATTTTACAAAAGTTCAGAAGACGGCGATAAATATGAAATATATAGATCCCACCTATATGATTCGAGCTATCCCAAGTAATGCGTCGGACAATATATACTGTACTCTTCTTGCTCAGAGTGCTGTTCATGGAGCCATGGCAGGATTCAGTGGTTTCACAGTCGGGCCTGTAAACAGTAGACATGCTTATATTCCAATTGCCCGTGTGACAGAGACGCAAAACACAGTAAAGTTGACAGATCGGATGTGGGCTAGACTTCTTGCATCAACGAACCAGCCTAGTTTCTTGGAATACAGTGAGGGGCAAGGACAAGCTAAAGAGGATGTTGATGTGATAAACAACAACATGAGAGCTACTTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003872 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008443 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009507 GO:0009536 GO:0009987 GO:0016052 GO:0016053 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019200 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044262 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046835 GO:0046939 GO:0051186 GO:0051188 GO:0055086 GO:0061615 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00030 ko00051 ko00052 ko00680 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko03018 ko04152 ko05230 map00010 map00030 map00051 map00052 map00680 map01100 map01110 map01120 map01130 map01200 map01230 map03018 map04152 map05230
Pfam Domains
Protein Families
PFK

Protein Analysis

541

Amino Acids

59.22

Weight (kDa)

8.5

Isoelectric Point (pI)

40.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PFK PF00365 157 - 463 1.8e-62 Phosphofructokinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1621
AasI GACNNNNNNGTC 1 cut(s) 242
Acc16I TGCGCA 1 cut(s) 372
AccB1I GGYRCC 1 cut(s) 659
AccBSI CCGCTC 1 cut(s) 1105
AccI GTMKAC 2 cut(s) 741, 1438
AccII CGCG 2 cut(s) 184, 654
AciI CCGC 5 cut(s) 133, 534, 654, 924, 1105
AclWI GGATC 6 cut(s) 78, 512, 826, 839, 1266, 1301
AcsI RAATTY 1 cut(s) 1091
AcvI CACGTG 1 cut(s) 483
AdeI CACNNNGTG 1 cut(s) 364
AfaI GTAC 1 cut(s) 1363
AfiI CCNNNNNNNGG 1 cut(s) 439
AflIII ACRYGT 4 cut(s) 482, 546, 928, 1123
AjiI CACGTC 1 cut(s) 422
AjuI GAANNNNNNNTTGG 2 cut(s) 552, 584
AluBI AGCT 9 cut(s) 144, 159, 213, 323, 914, 1216, 1328, 1574, 1613
AluI AGCT 9 cut(s) 144, 159, 213, 323, 914, 1216, 1328, 1574, 1613
Alw21I GWGCWC 1 cut(s) 215
Alw26I GTCTC 1 cut(s) 1466
AlwI GGATC 6 cut(s) 78, 512, 826, 839, 1266, 1301
AlwNI CAGNNNCTG 1 cut(s) 827
Ama87I CYCGRG 2 cut(s) 432, 499
AoxI GGCC 2 cut(s) 1072, 1424
ApeKI GCWGC 1 cut(s) 1038
ApoI RAATTY 1 cut(s) 1091
ArsI GACNNNNNNTTYG 4 cut(s) 382, 414, 860, 892
Asp700I GAANNNNTTC 1 cut(s) 255
AspLEI GCGC 3 cut(s) 186, 373, 654
AspS9I GGNCC 4 cut(s) 398, 428, 721, 1424
AsuC2I CCSGG 5 cut(s) 433, 434, 500, 501, 1076
AsuII TTCGAA 1 cut(s) 670
AvaI CYCGRG 2 cut(s) 432, 499
AvaII GGWCC 3 cut(s) 398, 428, 721
BaeI ACNNNNGTAYC 4 cut(s) 879, 912, 976, 1009
BamHI GGATCC 1 cut(s) 831
BanI GGYRCC 1 cut(s) 659
BanII GRGCYC 2 cut(s) 215, 356
BauI CACGAG 1 cut(s) 678
BbrPI CACGTG 1 cut(s) 483
BbsI GAAGAC 3 cut(s) 258, 803, 1286
Bbv12I GWGCWC 1 cut(s) 215
BbvI GCAGC 1 cut(s) 1025
BccI CCATC 2 cut(s) 752, 970
BceAI ACGGC 3 cut(s) 253, 274, 1299
BcgI CGANNNNNNTGC 2 cut(s) 350, 384
BcnI CCSGG 5 cut(s) 433, 434, 500, 501, 1076
BcoDI GTCTC 1 cut(s) 1466
BfaI CTAG 3 cut(s) 402, 1512, 1539
BglII AGATCT 1 cut(s) 778
BisI GCNGC 4 cut(s) 535, 924, 1039, 1106
BlsI GCNGC 4 cut(s) 536, 925, 1040, 1107
Bme1390I CCNGG 5 cut(s) 433, 434, 500, 501, 1076
Bme18I GGWCC 3 cut(s) 398, 428, 721
BmeT110I CYCGRG 2 cut(s) 432, 499
BmgBI CACGTC 1 cut(s) 422
BmgT120I GGNCC 4 cut(s) 398, 428, 721, 1424
BmiI GGNNCC 6 cut(s) 79, 399, 430, 661, 833, 1393
BmrFI CCNGG 5 cut(s) 433, 434, 500, 501, 1076
BmrI ACTGGG 1 cut(s) 82
BmsI GCATC 3 cut(s) 1189, 1211, 1532
BmuI ACTGGG 1 cut(s) 82
BpiI GAAGAC 3 cut(s) 258, 803, 1286
BpmI CTGGAG 1 cut(s) 64
Bpu14I TTCGAA 1 cut(s) 670
BpuMI CCSGG 5 cut(s) 433, 434, 500, 501, 1076
BsaAI YACGTR 1 cut(s) 483
BsaJI CCNNGG 8 cut(s) 163, 431, 432, 499, 500, 724, 1075, 1395
Bsc4I CCNNNNNNNGG 1 cut(s) 439
Bse118I RCCGGY 1 cut(s) 226
Bse1I ACTGG 1 cut(s) 88
Bse3DI GCAATG 1 cut(s) 996
BseDI CCNNGG 8 cut(s) 163, 431, 432, 499, 500, 724, 1075, 1395
BseGI GGATG 3 cut(s) 463, 1509, 1588
BseLI CCNNNNNNNGG 1 cut(s) 439
BseMI GCAATG 1 cut(s) 996
BseMII CTCAG 2 cut(s) 221, 1388
BseNI ACTGG 1 cut(s) 88
BseRI GAGGAG 4 cut(s) 19, 696, 786, 1211
BseXI GCAGC 1 cut(s) 1025
BseYI CCCAGC 2 cut(s) 140, 827
BsgI GTGCAG 1 cut(s) 444
Bsh1236I CGCG 2 cut(s) 184, 654
BshFI GGCC 2 cut(s) 1074, 1426
BshNI GGYRCC 1 cut(s) 659
BsiHKAI GWGCWC 1 cut(s) 215
BsiHKCI CYCGRG 2 cut(s) 432, 499
BsiSI CCGG 4 cut(s) 227, 433, 500, 1075
BslI CCNNNNNNNGG 1 cut(s) 439
BsmAI GTCTC 1 cut(s) 1466
BsmBI CGTCTC 1 cut(s) 1466
BsmI GAATGC 2 cut(s) 55, 1186
BsnI GGCC 2 cut(s) 1074, 1426
BsoBI CYCGRG 2 cut(s) 432, 499
Bsp119I TTCGAA 1 cut(s) 670
Bsp1286I GDGCHC 2 cut(s) 215, 356
Bsp143I GATC 8 cut(s) 83, 203, 504, 778, 831, 1258, 1306, 1498
Bsp19I CCATGG 1 cut(s) 1395
BspACI CCGC 5 cut(s) 133, 534, 654, 924, 1105
BspANI GGCC 2 cut(s) 1074, 1426
BspCNI CTCAG 2 cut(s) 220, 1387
BspFNI CGCG 2 cut(s) 184, 654
BspHI TCATGA 1 cut(s) 688
BspLI GGNNCC 6 cut(s) 79, 399, 430, 661, 833, 1393
BspPI GGATC 6 cut(s) 78, 512, 826, 839, 1266, 1301
BspQI GCTCTTC 1 cut(s) 1095
BspT104I TTCGAA 1 cut(s) 670
BspT107I GGYRCC 1 cut(s) 659
BsrBI CCGCTC 1 cut(s) 1105
BsrDI GCAATG 1 cut(s) 996
BsrFI RCCGGY 1 cut(s) 226
BsrI ACTGG 1 cut(s) 88
BssAI RCCGGY 1 cut(s) 226
BssECI CCNNGG 8 cut(s) 163, 431, 432, 499, 500, 724, 1075, 1395
BssMI GATC 8 cut(s) 83, 203, 504, 778, 831, 1258, 1306, 1498
BssNAI GTATAC 1 cut(s) 742
BssSI CACGAG 1 cut(s) 678
BssT1I CCWWGG 1 cut(s) 1395
Bst1107I GTATAC 1 cut(s) 742
Bst2BI CACGAG 1 cut(s) 678
Bst4CI ACNGT 7 cut(s) 514, 989, 1361, 1420, 1436, 1486, 1556
Bst6I CTCTTC 2 cut(s) 1095, 1371
BstBAI YACGTR 1 cut(s) 483
BstBI TTCGAA 1 cut(s) 670
BstC8I GCNNGC 2 cut(s) 228, 472
BstDEI CTNAG 3 cut(s) 207, 837, 1374
BstDSI CCRYGG 1 cut(s) 1395
BstF5I GGATG 3 cut(s) 463, 1509, 1588
BstFNI CGCG 2 cut(s) 184, 654
BstHHI GCGC 3 cut(s) 186, 373, 654
BstKTI GATC 8 cut(s) 86, 206, 507, 781, 834, 1261, 1309, 1501
BstMAI GTCTC 1 cut(s) 1466
BstMBI GATC 8 cut(s) 83, 203, 504, 778, 831, 1258, 1306, 1498
BstMWI GCNNNNNNNGC 9 cut(s) 52, 109, 141, 911, 920, 1007, 1016, 1148, 1379
BstNSI RCATGY 4 cut(s) 550, 932, 1127, 1445
BstSCI CCNGG 5 cut(s) 431, 432, 498, 499, 1074
BstUI CGCG 2 cut(s) 184, 654
BstV1I GCAGC 1 cut(s) 1025
BstV2I GAAGAC 3 cut(s) 258, 803, 1286
BstX2I RGATCY 4 cut(s) 83, 778, 831, 1306
BstYI RGATCY 4 cut(s) 83, 778, 831, 1306
BstZ17I GTATAC 1 cut(s) 742
BsuRI GGCC 2 cut(s) 1074, 1426
BtgI CCRYGG 1 cut(s) 1395
BtgZI GCGATG 1 cut(s) 771
BtrI CACGTC 1 cut(s) 422
BtsCI GGATG 3 cut(s) 463, 1509, 1588
BtsIMutI CAGTG 3 cut(s) 994, 1414, 1561
Cac8I GCNNGC 2 cut(s) 228, 472
CaiI CAGNNNCTG 1 cut(s) 827
CciI TCATGA 1 cut(s) 688
CfoI GCGC 3 cut(s) 186, 373, 654
Cfr10I RCCGGY 1 cut(s) 226
Cfr13I GGNCC 4 cut(s) 398, 428, 721, 1424
Cfr9I CCCGGG 2 cut(s) 432, 499
CseI GACGC 2 cut(s) 1332, 1483
Csp6I GTAC 1 cut(s) 1362
CviAII CATG 8 cut(s) 547, 689, 929, 1124, 1388, 1396, 1442, 1606
CviQI GTAC 1 cut(s) 1362
DdeI CTNAG 3 cut(s) 207, 837, 1374
DpnI GATC 8 cut(s) 85, 205, 506, 780, 833, 1260, 1308, 1500
DpnII GATC 8 cut(s) 83, 203, 504, 778, 831, 1258, 1306, 1498
DraIII CACNNNGTG 1 cut(s) 364
DrdI GACNNNNNNGTC 1 cut(s) 242
DseDI GACNNNNNNGTC 1 cut(s) 242
Eam1104I CTCTTC 2 cut(s) 1095, 1371
EarI CTCTTC 2 cut(s) 1095, 1371
Ecl136II GAGCTC 1 cut(s) 213
Eco130I CCWWGG 1 cut(s) 1395
Eco24I GRGCYC 2 cut(s) 215, 356
Eco32I GATATC 1 cut(s) 643
Eco47I GGWCC 3 cut(s) 398, 428, 721
Eco53kI GAGCTC 1 cut(s) 213
Eco72I CACGTG 1 cut(s) 483
Eco88I CYCGRG 2 cut(s) 432, 499
EcoICRI GAGCTC 1 cut(s) 213
EcoO109I RGGNCCY 2 cut(s) 398, 428
EcoRV GATATC 1 cut(s) 643
EcoT14I CCWWGG 1 cut(s) 1395
EcoT22I ATGCAT 1 cut(s) 1204
EcoT38I GRGCYC 2 cut(s) 215, 356
ErhI CCWWGG 1 cut(s) 1395
Esp3I CGTCTC 1 cut(s) 1466
FaeI CATG 8 cut(s) 550, 692, 932, 1127, 1391, 1399, 1445, 1609
FalI AAGNNNNNCTT 4 cut(s) 552, 584, 1092, 1124
FatI CATG 8 cut(s) 546, 688, 928, 1123, 1387, 1395, 1441, 1605
FblI GTMKAC 2 cut(s) 741, 1438
Fnu4HI GCNGC 4 cut(s) 535, 924, 1039, 1106
FokI GGATG 3 cut(s) 470, 1516, 1595
FriOI GRGCYC 2 cut(s) 215, 356
Fsp4HI GCNGC 4 cut(s) 535, 924, 1039, 1106
FspBI CTAG 3 cut(s) 402, 1512, 1539
FspI TGCGCA 1 cut(s) 372
GlaI GCGC 3 cut(s) 185, 372, 653
GluI GCNGC 4 cut(s) 535, 924, 1039, 1106
GsaI CCCAGC 2 cut(s) 144, 831
GsuI CTGGAG 1 cut(s) 64
HaeIII GGCC 2 cut(s) 1074, 1426
HapII CCGG 4 cut(s) 227, 433, 500, 1075
HgaI GACGC 2 cut(s) 1332, 1483
HhaI GCGC 3 cut(s) 186, 373, 654
Hin1II CATG 8 cut(s) 550, 692, 932, 1127, 1391, 1399, 1445, 1609
Hin6I GCGC 3 cut(s) 184, 371, 652
HinP1I GCGC 3 cut(s) 184, 371, 652
HincII GTYRAC 3 cut(s) 621, 1242, 1494
HindII GTYRAC 3 cut(s) 621, 1242, 1494
HindIII AAGCTT 2 cut(s) 157, 321
HinfI GANTC 7 cut(s) 5, 217, 517, 1045, 1052, 1321, 1404
HpaI GTTAAC 1 cut(s) 1242
HpaII CCGG 4 cut(s) 227, 433, 500, 1075
Hpy166II GTNNAC 7 cut(s) 621, 742, 949, 1242, 1432, 1439, 1494
Hpy188I TCNGA 9 cut(s) 167, 203, 210, 419, 457, 1278, 1348, 1377, 1503
Hpy188III TCNNGA 9 cut(s) 81, 153, 359, 521, 678, 689, 1025, 1049, 1206
Hpy8I GTNNAC 7 cut(s) 621, 742, 949, 1242, 1432, 1439, 1494
Hpy99I CGWCG 6 cut(s) 103, 112, 121, 239, 423, 1348
HpyAV CCTTC 3 cut(s) 184, 1064, 1139
HpyCH4III ACNGT 7 cut(s) 514, 989, 1361, 1420, 1436, 1486, 1556
HpyCH4IV ACGT 5 cut(s) 255, 421, 482, 611, 805
HpyCH4V TGCA 9 cut(s) 55, 329, 412, 425, 474, 1010, 1184, 1202, 1523
HpyF10VI GCNNNNNNNGC 9 cut(s) 52, 109, 141, 911, 920, 1007, 1016, 1148, 1379
HpyF3I CTNAG 3 cut(s) 207, 837, 1374
HpySE526I ACGT 5 cut(s) 255, 421, 482, 611, 805
Hsp92II CATG 8 cut(s) 550, 692, 932, 1127, 1391, 1399, 1445, 1609
HspAI GCGC 3 cut(s) 184, 371, 652
KroI GCCGGC 1 cut(s) 226
KroNI GCCGGC 1 cut(s) 228
KspAI GTTAAC 1 cut(s) 1242
Kzo9I GATC 8 cut(s) 83, 203, 504, 778, 831, 1258, 1306, 1498
LguI GCTCTTC 1 cut(s) 1095
LmnI GCTCC 5 cut(s) 83, 108, 773, 1148, 1391
Lsp1109I GCAGC 1 cut(s) 1025
LweI GCATC 3 cut(s) 1189, 1211, 1532
MaeI CTAG 3 cut(s) 402, 1512, 1539
MaeII ACGT 5 cut(s) 255, 421, 482, 611, 805
MaeIII GTNAC 2 cut(s) 478, 1465
MalI GATC 8 cut(s) 85, 205, 506, 780, 833, 1260, 1308, 1500
MbiI CCGCTC 1 cut(s) 1105
MboI GATC 8 cut(s) 83, 203, 504, 778, 831, 1258, 1306, 1498
MboII GAAGA 8 cut(s) 263, 572, 659, 803, 911, 1112, 1291, 1358
MfeI CAATTG 2 cut(s) 843, 1455
MflI RGATCY 4 cut(s) 83, 778, 831, 1306
MhlI GDGCHC 2 cut(s) 215, 356
MluCI AATT 8 cut(s) 271, 283, 573, 747, 843, 963, 1091, 1455
MmeI TCCRAC 4 cut(s) 435, 442, 940, 1326
Mph1103I ATGCAT 1 cut(s) 1204
MroNI GCCGGC 1 cut(s) 226
MroXI GAANNNNTTC 1 cut(s) 255
MseI TTAA 4 cut(s) 918, 1110, 1241, 1254
MslI CAYNNNNRTG 1 cut(s) 1316
MspI CCGG 4 cut(s) 227, 433, 500, 1075
MspR9I CCNGG 5 cut(s) 433, 434, 500, 501, 1076
MunI CAATTG 2 cut(s) 843, 1455
Mva1269I GAATGC 2 cut(s) 55, 1186
MvnI CGCG 2 cut(s) 184, 654
MwoI GCNNNNNNNGC 9 cut(s) 52, 109, 141, 911, 920, 1007, 1016, 1148, 1379
NaeI GCCGGC 1 cut(s) 228
NciI CCSGG 5 cut(s) 433, 434, 500, 501, 1076
NcoI CCATGG 1 cut(s) 1395
NdeII GATC 8 cut(s) 83, 203, 504, 778, 831, 1258, 1306, 1498
NgoMIV GCCGGC 1 cut(s) 226
NlaIII CATG 8 cut(s) 550, 692, 932, 1127, 1391, 1399, 1445, 1609
NlaIV GGNNCC 6 cut(s) 79, 399, 430, 661, 833, 1393
NmeAIII GCCGAG 1 cut(s) 54
NmuCI GTSAC 2 cut(s) 478, 1465
NsbI TGCGCA 1 cut(s) 372
NsiI ATGCAT 1 cut(s) 1204
NspI RCATGY 4 cut(s) 550, 932, 1127, 1445
NspV TTCGAA 1 cut(s) 670
PagI TCATGA 1 cut(s) 688
PciI ACATGT 3 cut(s) 546, 928, 1123
PciSI GCTCTTC 1 cut(s) 1095
PcsI WCGNNNNNNNCGW 2 cut(s) 240, 676
PctI GAATGC 2 cut(s) 55, 1186
PdiI GCCGGC 1 cut(s) 228
PdmI GAANNNNTTC 1 cut(s) 255
PfeI GAWTC 7 cut(s) 5, 217, 517, 1045, 1052, 1321, 1404
PkrI GCNGC 4 cut(s) 536, 925, 1040, 1107
PmaCI CACGTG 1 cut(s) 483
PmlI CACGTG 1 cut(s) 483
Ppu21I YACGTR 1 cut(s) 483
PpuMI RGGWCCY 2 cut(s) 398, 428
PscI ACATGT 3 cut(s) 546, 928, 1123
PsiI TTATAA 1 cut(s) 1621
Psp124BI GAGCTC 1 cut(s) 215
Psp5II RGGWCCY 2 cut(s) 398, 428
PspCI CACGTG 1 cut(s) 483
PspFI CCCAGC 2 cut(s) 140, 827
PspN4I GGNNCC 6 cut(s) 79, 399, 430, 661, 833, 1393
PspPI GGNCC 4 cut(s) 398, 428, 721, 1424
PspPPI RGGWCCY 2 cut(s) 398, 428
PstNI CAGNNNCTG 1 cut(s) 827
PsuI RGATCY 4 cut(s) 83, 778, 831, 1306
RsaI GTAC 1 cut(s) 1363
RsaNI GTAC 1 cut(s) 1362
RseI CAYNNNNRTG 1 cut(s) 1316
SacI GAGCTC 1 cut(s) 215
SapI GCTCTTC 1 cut(s) 1095
SaqAI TTAA 4 cut(s) 918, 1110, 1241, 1254
SatI GCNGC 4 cut(s) 535, 924, 1039, 1106
Sau3AI GATC 8 cut(s) 83, 203, 504, 778, 831, 1258, 1306, 1498
Sau96I GGNCC 4 cut(s) 398, 428, 721, 1424
ScrFI CCNGG 5 cut(s) 433, 434, 500, 501, 1076
SduI GDGCHC 2 cut(s) 215, 356
SfaNI GCATC 3 cut(s) 1189, 1211, 1532
SfuI TTCGAA 1 cut(s) 670
SinI GGWCC 3 cut(s) 398, 428, 721
SmaI CCCGGG 2 cut(s) 434, 501
SmiMI CAYNNNNRTG 1 cut(s) 1316
Sse9I AATT 8 cut(s) 271, 283, 573, 747, 843, 963, 1091, 1455
SsiI CCGC 5 cut(s) 133, 534, 654, 924, 1105
SspMI CTAG 3 cut(s) 402, 1512, 1539
SstI GAGCTC 1 cut(s) 215
StyD4I CCNGG 5 cut(s) 431, 432, 498, 499, 1074
StyI CCWWGG 1 cut(s) 1395
TaaI ACNGT 7 cut(s) 514, 989, 1361, 1420, 1436, 1486, 1556
TaiI ACGT 5 cut(s) 258, 424, 485, 614, 808
TaqI TCGA 8 cut(s) 215, 249, 670, 885, 1024, 1098, 1224, 1324
TaqII GACCGA 1 cut(s) 738
TasI AATT 8 cut(s) 271, 283, 573, 747, 843, 963, 1091, 1455
TatI WGTACW 1 cut(s) 1361
TauI GCSGC 3 cut(s) 537, 926, 1108
TfiI GAWTC 7 cut(s) 5, 217, 517, 1045, 1052, 1321, 1404
Tru1I TTAA 4 cut(s) 918, 1110, 1241, 1254
Tru9I TTAA 4 cut(s) 918, 1110, 1241, 1254
TscAI CASTG 3 cut(s) 994, 1414, 1561
TseFI GTSAC 2 cut(s) 478, 1465
TseI GCWGC 1 cut(s) 1038
Tsp45I GTSAC 2 cut(s) 478, 1465
TspDTI ATGAA 6 cut(s) 6, 474, 985, 1193, 1310, 1376
TspGWI ACGGA 3 cut(s) 87, 105, 167
TspMI CCCGGG 2 cut(s) 432, 499
TspRI CASTG 3 cut(s) 994, 1414, 1561
VpaK11BI GGWCC 3 cut(s) 398, 428, 721
XapI RAATTY 1 cut(s) 1091
XceI RCATGY 4 cut(s) 550, 932, 1127, 1445
XcmI CCANNNNNNNNNTGG 1 cut(s) 653
XmaI CCCGGG 2 cut(s) 432, 499
XmiI GTMKAC 2 cut(s) 741, 1438
XmnI GAANNNNTTC 1 cut(s) 255
XspI CTAG 3 cut(s) 402, 1512, 1539
Zsp2I ATGCAT 1 cut(s) 1204
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.