Rorug01G0378800

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0008946
Physical Location & Seq
Reverse (-)
49210361 .. 49213411
3051 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0378800.1

Sequence Viewer

Length: 3051 bp
ATGGCAGAAGTACTTGTTAACGTTCTCCTTGAGCGTTTGGCTACGATCACCCTTGACAAGGTCAGAGGAGAGTTCAAACTGGTCACTGGTGTTGAGAAAGAAGTCAAGAGCCTCACCCGCAATCTCGAAGCTATTCAAGCTGTGCTCGAGGATGCAGAGCAGAGGCAAGTGACGGAGCCAACTGTGAGACGTTGGCTGAGTGAACTGACTGAAGTTTCCTTCGACATGGATGACGTGTTGGATGAGTGGATAACTGAAGTTCTGAAGCAACAAATGGAGAAACAAGAAAAACAAGTTGAAGATGCTCTTATTGTTACTACTAAGAAGAAGGTATGCTTCCCCTTCCTTTCCTCTTGCTTTTGTTTCGGCCAAGTCAATCAGTTAATGCTTCGTCATGATATTGCTGTGAGGATAAAAGACCTAAATGAAAGATTAGCTTCAATTGATGAAAGAAAACAAAGTTTTGGCTTTCAAAACATCACAAGGGTCTCCGAATTACCTGAACGACAAAAAACTACTTCTCTTCCTAATGTTAAAACATTCGGTCGAGATAAGGAAAAAAGCCTCATAGCCAGCAAGCTGCTGAGTGAGAGTAGTGAGGGAAATGAGGTTCCTCTTATCATCCCTATTGTAGGGATGGGGGGAATGGGGAAAACAACTCTTGCTCAACTTGTTTATAATGATGAAGAGGTCAAATCTCATTTTGACAAGAGAATATGGGTCTGTGTCTCAGACCCTTTTGAAGAGATCAAGATTGCTCAAGCTATCATTGAAGAAATAGATACAGATAATAGTTCAAAAAGCTCAAATGTGTTGCAAACCCTGACGCAATGTATATATAAATTGATTGAGGGTAAAAAGTTTCTCCTGGTTTTAGATGATGTTTGGAGCCCAAACTCTGATCAATGGGAAGAATTGATCAAACCTTTACGTTATGGTGCTACTGGTAGTAGAGTATTGGTGACCACTAGAAAGGAGGAGGTTGCTACTTTAATGAAAGCAACAACGACTCAAATGATCTCTTTGAAGGCATTGAGTGATGCATTTTGTTTGTCATTGTTCTATTACAGTGCAAACATGGACGAGAATAATGTGTCTAAAGAGTTTAAAGATATTGGATTGAAGATTGTGAAAAGGTGCAATGGGTTGCCTCTTGCTGCAAAGACATTAGGAAGCCTCATGCGCAATAAGAAAAAGATTCATGAATGGCAAGCTGTTTTGAACAGTAAGACATGGGAATTGAAAGAAATTCAACAAGATGTTTTTCGACCACTACTACTAAGTTATCATGATTTGACTCCAGCAATCAAACGTTGTCTTTTGTATTGTGTTACATTTCCAAAAGATTATGTGTACAATATGAATTGTTTGATTGAGTTGTGGATGTCGCAGGATTATTTGAATGCCAAAGAAAGTAAAGAAAAGATAGTAGTTGGTCAAAATTATTTTGATGACTTAGCAATGCGGTCATTCTTTCAAGATATTGAGGTAGATGATGAGGATGAGTATGGAAACATAAGGTGCAAAATTCATGATATTGTACATGACTTTCTACAATATTTGACCAAGGATGAATGTTTGATTTTGGATTTTGAGTTGGATGCTAGTGAGAGAAGGGATGTGTCAAAGGATAACATTCGACATTTGACTTTAGTGCACGCAATAGAGAATGAGTCATTGGCACATTTTCTCCTCAATTGTAAAAAATTACGTACCTTGATGGCTATAGATTCATTCAAATATTCCATTGGCCCAATATTTGAGTTGACAGTACAATTGAAATGCCTTAGAACATTAAGCTTGTGTGGTGGGTGGGGGGAGAGTTGTCTCAGAGAAATTCCCGAGACGATAGGTGGATTGATACATTTGAGGTATCTTGATTTATCCGGTAATGAATACTTGAAAGAATTACCCAGCGCCCTGGGCAGCTTATACAACCTGCAAACCTTGCGACTTGTTTATTGCTCTAGTCTCAAAGAAATTCCCGAGACGATCGGTGGATTAATACATTTGAGGTATCTTGATTTATCCTTTAACACCGAGTTGAAAGAATTACCCAGCGCCCTGAGCAGCTTATCCAACCTGCAAACCTTGCGACTTGTTGGTTGCGAGATTCTCGAAAAAGTAGATGTGCGAAGGCTGATTAACTTGAGGCATCTTTATGTTGAAGGCTGTGAGAAGCTAGAGTTAATTAAAGGGATTGAGAAATTAACAGATCTGCAAAGGCTAGATGTGTTTCTTGTCCCATTTATTGGTGGTGATGAAGGAAACAAGTTGGAAGATCTGAAAGACTTGAACCAGCTTCAAGGGAGTCTTGATATTTATATTCGAGGAAATCCAAATATGGCGGAGAATGCAGCAGCAATTATGGTGAACAAGGCTCACCTCCTGCAATTGAGGCTATCTTTCCCTTTGGATTCTGATGAAGGAGAGAGTAGAGAAATTATGAATGGCTTAGAGCCGCATCCAAGTTTGGAATCTTTATCCATCGTTGGGTATAGAGGCGGCGCCTTCTCCCATTGGTTGTCGTCTTTAAACAGCTTGAGAGTGCTCACCCTTCGTTGTTGCGGCAAATGTGGGGTTTTGCCTCCTTTAGGGAAACTGGCGTCCCTTGAATCATTGGATATTTCGAGTGTCTGGGAACTTAAAAAGGTAGGAGTTGAGTTTTTGGGAATAGATGATGGGCAAACCTCGTCGTCTTCCATTTTCATATCATTCCCCAAGCTCAAACAACTCACCATCGAAGACATGGAGTCATGGGAAGAGTGGGAAGGAGTGGAAAATAATAATATTACAATCATGCCATGCCTCTCTCAGTTGTCAATTGAGAGTTGCCATCACCTGAAAGCACTGCCGGACTTCCTGTGGAAGACACCACTACAGAAATTGCACATGGACAGATCTCCAATTCTCCAAGACCTTTACAGACAAGGAACTGGTGAGGAGTGGGCCAAGATTTCTCACATCCCAAGCATCAAGATAGAAGCCCTGATCTGGAAGGTGACGCATCATCCCTCGATGAGTGAAAAGGTACATTTGCTTCTAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0003872 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008443 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009507 GO:0009536 GO:0009987 GO:0016052 GO:0016053 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019200 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044262 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046835 GO:0046939 GO:0051186 GO:0051188 GO:0055086 GO:0061615 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00030 ko00051 ko00052 ko00680 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko03018 ko04152 ko05230 map00010 map00030 map00051 map00052 map00680 map01100 map01110 map01120 map01130 map01200 map01230 map03018 map04152 map05230
Pfam Domains
Protein Families
PFK

Protein Analysis

1016

Amino Acids

115.79

Weight (kDa)

5.4

Isoelectric Point (pI)

44.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 6 - 96 2.3e-24 Rx N-terminal domain
NB-ARC PF00931 190 - 361 1.4e-37 NB-ARC domain
WHD_DRP PF23559 446 - 517 7.8e-20 Disease resistance protein Winged helix domain
LRR_14 PF23598 589 - 668 1e-09 Leucine-rich repeat region
LRR_8 PF13855 646 - 705 4.1e-06 Leucine rich repeat
LRR_14 PF23598 664 - 940 4.8e-20 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 759 - 878 7.1e-26 R13L1/DRL21 LRRs
LRR_RPS2 PF23247 844 - 963 1e-05 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 678
Acc16I TGCGCA 1 cut(s) 1184
Acc36I ACCTGC 2 cut(s) 1947, 2091
AccB1I GGYRCC 1 cut(s) 2507
AccB7I CCANNNNNTGG 1 cut(s) 2252
AciI CCGC 6 cut(s) 118, 1465, 2348, 2462, 2505, 2568
AclI AACGTT 2 cut(s) 21, 1312
AcoI YGGCCR 1 cut(s) 367
AcsI RAATTY 4 cut(s) 1248, 1527, 1836, 1980
AcuI CTGAAG 3 cut(s) 231, 276, 284
AcyI GRCGYC 2 cut(s) 2508, 2606
AfaI GTAC 6 cut(s) 12, 1355, 1542, 1714, 1773, 3033
AfiI CCNNNNNNNGG 6 cut(s) 58, 632, 2252, 2493, 2594, 2994
AflIII ACRYGT 1 cut(s) 234
AhdI GACNNNNNGTC 1 cut(s) 2752
AjiI CACGTC 1 cut(s) 235
AjnI CCWGG 2 cut(s) 867, 1920
AjuI GAANNNNNNNTTGG 2 cut(s) 1661, 1693
AloI GAACNNNNNNTCC 2 cut(s) 594, 626
Alw21I GWGCWC 3 cut(s) 147, 1659, 2553
Alw26I GTCTC 7 cut(s) 181, 493, 733, 1832, 1838, 1976, 1982
Alw44I GTGCAC 1 cut(s) 1655
Ama87I CYCGRG 3 cut(s) 146, 1841, 1985
AoxI GGCC 3 cut(s) 367, 1750, 2949
ApaLI GTGCAC 1 cut(s) 1655
ApeKI GCWGC 6 cut(s) 580, 1157, 1926, 2070, 2357, 2360
ApoI RAATTY 4 cut(s) 1248, 1527, 1836, 1980
AseI ATTAAT 1 cut(s) 2003
Asp700I GAANNNNTTC 1 cut(s) 132
AspLEI GCGC 4 cut(s) 1185, 1919, 2063, 2510
AspS9I GGNCC 2 cut(s) 1751, 2949
AvaI CYCGRG 3 cut(s) 146, 1841, 1985
BaeGI GKGCMC 1 cut(s) 1659
BanI GGYRCC 1 cut(s) 2507
BanII GRGCYC 1 cut(s) 893
BarI GAAGNNNNNNTAC 1 cut(s) 3024
BbsI GAAGAC 3 cut(s) 2691, 2751, 2876
Bbv12I GWGCWC 3 cut(s) 147, 1659, 2553
BbvI GCAGC 6 cut(s) 567, 1144, 1938, 2082, 2369, 2372
BccI CCATC 6 cut(s) 631, 1714, 2495, 2675, 2747, 2844
BciT130I CCWGG 2 cut(s) 869, 1922
BclI TGATCA 2 cut(s) 901, 918
BcoDI GTCTC 7 cut(s) 181, 493, 733, 1832, 1838, 1976, 1982
BfaI CTAG 6 cut(s) 969, 1605, 1968, 2183, 2228, 3044
BfmI CTRYAG 2 cut(s) 1725, 2879
BfoI RGCGCY 3 cut(s) 1920, 2064, 2511
BfuAI ACCTGC 2 cut(s) 1947, 2091
BglII AGATCT 3 cut(s) 2215, 2281, 2900
BisI GCNGC 9 cut(s) 581, 1158, 1927, 2071, 2358, 2361, 2462, 2506, 2569
BlsI GCNGC 9 cut(s) 582, 1159, 1928, 2072, 2359, 2362, 2463, 2507, 2570
BmcAI AGTACT 1 cut(s) 12
Bme1390I CCNGG 2 cut(s) 869, 1922
BmeRI GACNNNNNGTC 1 cut(s) 2752
BmeT110I CYCGRG 3 cut(s) 146, 1841, 1985
BmgBI CACGTC 1 cut(s) 235
BmgT120I GGNCC 2 cut(s) 1751, 2949
BmiI GGNNCC 4 cut(s) 177, 612, 890, 2509
BmrFI CCNGG 2 cut(s) 869, 1922
BmsI GCATC 8 cut(s) 142, 292, 1030, 1591, 2164, 2473, 2982, 3016
BpiI GAAGAC 3 cut(s) 2691, 2751, 2876
BpmI CTGGAG 1 cut(s) 1284
Bpu10I CCTNAGC 1 cut(s) 2066
BpuEI CTTGAG 4 cut(s) 50, 744, 2170, 2563
BsaAI YACGTR 1 cut(s) 1712
BsaHI GRCGYC 2 cut(s) 2508, 2606
BsaI GGTCTC 1 cut(s) 493
BsaJI CCNNGG 3 cut(s) 1566, 1920, 1921
BsaWI WCCGGW 1 cut(s) 1886
BsaXI ACNNNNNCTCC 2 cut(s) 1674, 1704
Bsc4I CCNNNNNNNGG 6 cut(s) 58, 632, 2252, 2493, 2594, 2994
Bse1I ACTGG 5 cut(s) 84, 91, 949, 2607, 2941
Bse3DI GCAATG 3 cut(s) 836, 1147, 1467
BseBI CCWGG 2 cut(s) 869, 1922
BseDI CCNNGG 3 cut(s) 1566, 1920, 1921
BseLI CCNNNNNNNGG 6 cut(s) 58, 632, 2252, 2493, 2594, 2994
BseMI GCAATG 3 cut(s) 836, 1147, 1467
BseMII CTCAG 6 cut(s) 188, 575, 744, 1843, 2057, 2828
BseNI ACTGG 5 cut(s) 84, 91, 949, 2607, 2941
BseRI GAGGAG 4 cut(s) 81, 992, 1682, 2957
BseSI GKGCMC 1 cut(s) 1659
BseXI GCAGC 6 cut(s) 567, 1144, 1938, 2082, 2369, 2372
BseYI CCCAGC 2 cut(s) 1913, 2057
Bsh1285I CGRYCG 2 cut(s) 547, 1995
BshFI GGCC 3 cut(s) 369, 1752, 2951
BshNI GGYRCC 1 cut(s) 2507
BsiEI CGRYCG 2 cut(s) 547, 1995
BsiHKAI GWGCWC 3 cut(s) 147, 1659, 2553
BsiHKCI CYCGRG 3 cut(s) 146, 1841, 1985
BsiSI CCGG 2 cut(s) 1887, 2855
BslFI GGGAC 2 cut(s) 2228, 2593
BslI CCNNNNNNNGG 6 cut(s) 58, 632, 2252, 2493, 2594, 2994
BsmAI GTCTC 7 cut(s) 181, 493, 733, 1832, 1838, 1976, 1982
BsmBI CGTCTC 3 cut(s) 181, 1838, 1982
BsmFI GGGAC 2 cut(s) 2228, 2593
BsmI GAATGC 2 cut(s) 1408, 2359
BsnI GGCC 3 cut(s) 369, 1752, 2951
Bso31I GGTCTC 1 cut(s) 493
BsoBI CYCGRG 3 cut(s) 146, 1841, 1985
Bsp1286I GDGCHC 4 cut(s) 147, 893, 1659, 2553
Bsp1407I TGTACA 2 cut(s) 1353, 1540
BspACI CCGC 6 cut(s) 118, 1465, 2348, 2462, 2505, 2568
BspANI GGCC 3 cut(s) 369, 1752, 2951
BspCNI CTCAG 6 cut(s) 189, 576, 743, 1842, 2058, 2827
BspHI TCATGA 4 cut(s) 394, 1201, 1288, 1531
BspLI GGNNCC 4 cut(s) 177, 612, 890, 2509
BspMI ACCTGC 2 cut(s) 1947, 2091
BspT107I GGYRCC 1 cut(s) 2507
BspTNI GGTCTC 1 cut(s) 493
BsrDI GCAATG 3 cut(s) 836, 1147, 1467
BsrGI TGTACA 2 cut(s) 1353, 1540
BsrI ACTGG 5 cut(s) 84, 91, 949, 2607, 2941
BssECI CCNNGG 3 cut(s) 1566, 1920, 1921
BssNI GRCGYC 2 cut(s) 2508, 2606
BssT1I CCWWGG 1 cut(s) 1566
Bst2UI CCWGG 2 cut(s) 869, 1922
Bst4CI ACNGT 4 cut(s) 184, 1070, 1226, 1771
Bst6I CTCTTC 4 cut(s) 528, 681, 738, 2757
BstACI GRCGYC 2 cut(s) 2508, 2606
BstAPI GCANNNNNTGC 2 cut(s) 1948, 2092
BstAUI TGTACA 2 cut(s) 1353, 1540
BstBAI YACGTR 1 cut(s) 1712
BstC8I GCNNGC 4 cut(s) 574, 578, 1212, 1659
BstEII GGTNACC 1 cut(s) 961
BstENI CCTNNNNNAGG 3 cut(s) 56, 630, 2592
BstH2I RGCGCY 3 cut(s) 1920, 2064, 2511
BstHHI GCGC 4 cut(s) 1185, 1919, 2063, 2510
BstMAI GTCTC 7 cut(s) 181, 493, 733, 1832, 1838, 1976, 1982
BstMCI CGRYCG 2 cut(s) 547, 1995
BstMWI GCNNNNNNNGC 9 cut(s) 117, 137, 1182, 1923, 1948, 2067, 2092, 2354, 2398
BstNI CCWGG 2 cut(s) 869, 1922
BstPI GGTNACC 1 cut(s) 961
BstSCI CCNGG 2 cut(s) 867, 1920
BstSFI CTRYAG 2 cut(s) 1725, 2879
BstSLI GKGCMC 1 cut(s) 1659
BstSNI TACGTA 1 cut(s) 1712
BstV1I GCAGC 6 cut(s) 567, 1144, 1938, 2082, 2369, 2372
BstV2I GAAGAC 3 cut(s) 2691, 2751, 2876
BstX2I RGATCY 3 cut(s) 2215, 2281, 2900
BstXI CCANNNNNNTGG 1 cut(s) 1921
BstYI RGATCY 3 cut(s) 2215, 2281, 2900
BsuRI GGCC 3 cut(s) 369, 1752, 2951
BtrI CACGTC 1 cut(s) 235
BtsI GCAGTG 1 cut(s) 2849
BtsIMutI CAGTG 3 cut(s) 84, 1075, 2849
BveI ACCTGC 2 cut(s) 1947, 2091
Cac8I GCNNGC 4 cut(s) 574, 578, 1212, 1659
CciI TCATGA 4 cut(s) 394, 1201, 1288, 1531
CfoI GCGC 4 cut(s) 1185, 1919, 2063, 2510
Cfr13I GGNCC 2 cut(s) 1751, 2949
CseI GACGC 3 cut(s) 835, 2595, 3013
Csp6I GTAC 6 cut(s) 11, 1354, 1541, 1713, 1772, 3032
CviQI GTAC 6 cut(s) 11, 1354, 1541, 1713, 1772, 3032
DinI GGCGCC 1 cut(s) 2509
DraI TTTAAA 2 cut(s) 1108, 2535
DriI GACNNNNNGTC 1 cut(s) 2752
EaeI YGGCCR 1 cut(s) 367
Eam1104I CTCTTC 4 cut(s) 528, 681, 738, 2757
Eam1105I GACNNNNNGTC 1 cut(s) 2752
EarI CTCTTC 4 cut(s) 528, 681, 738, 2757
EciI GGCGGA 1 cut(s) 2363
Eco105I TACGTA 1 cut(s) 1712
Eco130I CCWWGG 1 cut(s) 1566
Eco24I GRGCYC 1 cut(s) 893
Eco31I GGTCTC 1 cut(s) 493
Eco57I CTGAAG 3 cut(s) 231, 276, 284
Eco88I CYCGRG 3 cut(s) 146, 1841, 1985
Eco91I GGTNACC 1 cut(s) 961
EcoNI CCTNNNNNAGG 3 cut(s) 56, 630, 2592
EcoO65I GGTNACC 1 cut(s) 961
EcoRII CCWGG 2 cut(s) 867, 1920
EcoT14I CCWWGG 1 cut(s) 1566
EcoT22I ATGCAT 1 cut(s) 1045
EcoT38I GRGCYC 1 cut(s) 893
EgeI GGCGCC 1 cut(s) 2509
EheI GGCGCC 1 cut(s) 2509
ErhI CCWWGG 1 cut(s) 1566
Esp3I CGTCTC 3 cut(s) 181, 1838, 1982
FalI AAGNNNNNCTT 8 cut(s) 291, 323, 320, 352, 421, 453, 2298, 2330
FaqI GGGAC 2 cut(s) 2228, 2593
FauI CCCGC 1 cut(s) 125
FbaI TGATCA 2 cut(s) 901, 918
Fnu4HI GCNGC 9 cut(s) 581, 1158, 1927, 2071, 2358, 2361, 2462, 2506, 2569
FriOI GRGCYC 1 cut(s) 893
Fsp4HI GCNGC 9 cut(s) 581, 1158, 1927, 2071, 2358, 2361, 2462, 2506, 2569
FspBI CTAG 6 cut(s) 969, 1605, 1968, 2183, 2228, 3044
FspI TGCGCA 1 cut(s) 1184
GlaI GCGC 4 cut(s) 1184, 1918, 2062, 2509
GluI GCNGC 9 cut(s) 581, 1158, 1927, 2071, 2358, 2361, 2462, 2506, 2569
GsaI CCCAGC 2 cut(s) 1917, 2061
GsuI CTGGAG 1 cut(s) 1284
HaeII RGCGCY 3 cut(s) 1920, 2064, 2511
HaeIII GGCC 3 cut(s) 369, 1752, 2951
HapII CCGG 2 cut(s) 1887, 2855
HgaI GACGC 3 cut(s) 835, 2595, 3013
HhaI GCGC 4 cut(s) 1185, 1919, 2063, 2510
Hin1I GRCGYC 2 cut(s) 2508, 2606
Hin6I GCGC 4 cut(s) 1183, 1917, 2061, 2508
HinP1I GCGC 4 cut(s) 1183, 1917, 2061, 2508
HincII GTYRAC 2 cut(s) 19, 1767
HindII GTYRAC 2 cut(s) 19, 1767
HindIII AAGCTT 1 cut(s) 1798
HpaI GTTAAC 1 cut(s) 19
HpaII CCGG 2 cut(s) 1887, 2855
Hpy166II GTNNAC 6 cut(s) 19, 203, 1354, 1657, 1767, 2374
Hpy188I TCNGA 8 cut(s) 65, 264, 493, 733, 901, 1832, 2286, 2422
Hpy8I GTNNAC 6 cut(s) 19, 203, 1354, 1657, 1767, 2374
Hpy99I CGWCG 1 cut(s) 2698
HpyCH4III ACNGT 4 cut(s) 184, 1070, 1226, 1771
HpyCH4IV ACGT 6 cut(s) 21, 190, 234, 931, 1312, 1711
HpyF10VI GCNNNNNNNGC 9 cut(s) 117, 137, 1182, 1923, 1948, 2067, 2092, 2354, 2398
HpySE526I ACGT 6 cut(s) 21, 190, 234, 931, 1312, 1711
Hsp92I GRCGYC 2 cut(s) 2508, 2606
HspAI GCGC 4 cut(s) 1183, 1917, 2061, 2508
KasI GGCGCC 1 cut(s) 2507
Ksp22I TGATCA 2 cut(s) 901, 918
KspAI GTTAAC 1 cut(s) 19
LmnI GCTCC 2 cut(s) 175, 888
Lsp1109I GCAGC 6 cut(s) 567, 1144, 1938, 2082, 2369, 2372
LweI GCATC 8 cut(s) 142, 292, 1030, 1591, 2164, 2473, 2982, 3016
MaeI CTAG 6 cut(s) 969, 1605, 1968, 2183, 2228, 3044
MaeII ACGT 6 cut(s) 21, 190, 234, 931, 1312, 1711
MaeIII GTNAC 6 cut(s) 82, 169, 313, 961, 1330, 3001
MfeI CAATTG 5 cut(s) 441, 1696, 1775, 2393, 2823
MflI RGATCY 3 cut(s) 2215, 2281, 2900
MhlI GDGCHC 4 cut(s) 147, 893, 1659, 2553
Mly113I GGCGCC 1 cut(s) 2508
MlyI GAGTC 5 cut(s) 1003, 1291, 1682, 2320, 2762
MmeI TCCRAC 4 cut(s) 219, 1578, 2103, 2256
Mph1103I ATGCAT 1 cut(s) 1045
MroXI GAANNNNTTC 1 cut(s) 132
MslI CAYNNNNRTG 1 cut(s) 2160
MspI CCGG 2 cut(s) 1887, 2855
MspR9I CCNGG 2 cut(s) 869, 1922
MunI CAATTG 5 cut(s) 441, 1696, 1775, 2393, 2823
Mva1269I GAATGC 2 cut(s) 1408, 2359
MvaI CCWGG 2 cut(s) 869, 1922
MwoI GCNNNNNNNGC 9 cut(s) 117, 137, 1182, 1923, 1948, 2067, 2092, 2354, 2398
NarI GGCGCC 1 cut(s) 2508
NlaIV GGNNCC 4 cut(s) 177, 612, 890, 2509
NmuCI GTSAC 4 cut(s) 82, 169, 961, 3001
NsbI TGCGCA 1 cut(s) 1184
NsiI ATGCAT 1 cut(s) 1045
PacI TTAATTAA 1 cut(s) 2193
PaeR7I CTCGAG 1 cut(s) 146
PagI TCATGA 4 cut(s) 394, 1201, 1288, 1531
PasI CCCWGGG 1 cut(s) 1921
PctI GAATGC 2 cut(s) 1408, 2359
PdmI GAANNNNTTC 1 cut(s) 132
PfeI GAWTC 6 cut(s) 1198, 1730, 2113, 2417, 2477, 2615
PflFI GACNNNGTC 1 cut(s) 59
PflMI CCANNNNNTGG 1 cut(s) 2252
PkrI GCNGC 9 cut(s) 582, 1159, 1928, 2072, 2359, 2362, 2463, 2507, 2570
Ple19I CGATCG 1 cut(s) 1995
PleI GAGTC 5 cut(s) 1003, 1291, 1681, 2319, 2761
PluTI GGCGCC 1 cut(s) 2511
PpsI GAGTC 5 cut(s) 1003, 1291, 1681, 2319, 2761
Ppu21I YACGTR 1 cut(s) 1712
PshBI ATTAAT 1 cut(s) 2003
PsiI TTATAA 1 cut(s) 678
Psp1406I AACGTT 2 cut(s) 21, 1312
Psp6I CCWGG 2 cut(s) 867, 1920
PspEI GGTNACC 1 cut(s) 961
PspFI CCCAGC 2 cut(s) 1913, 2057
PspGI CCWGG 2 cut(s) 867, 1920
PspN4I GGNNCC 4 cut(s) 177, 612, 890, 2509
PspPI GGNCC 2 cut(s) 1751, 2949
PspXI VCTCGAGB 1 cut(s) 146
PsuI RGATCY 3 cut(s) 2215, 2281, 2900
PsyI GACNNNGTC 1 cut(s) 59
PvuI CGATCG 1 cut(s) 1995
RsaI GTAC 6 cut(s) 12, 1355, 1542, 1714, 1773, 3033
RsaNI GTAC 6 cut(s) 11, 1354, 1541, 1713, 1772, 3032
RseI CAYNNNNRTG 1 cut(s) 2160
SatI GCNGC 9 cut(s) 581, 1158, 1927, 2071, 2358, 2361, 2462, 2506, 2569
Sau96I GGNCC 2 cut(s) 1751, 2949
ScaI AGTACT 1 cut(s) 12
SchI GAGTC 5 cut(s) 1003, 1291, 1682, 2320, 2762
ScrFI CCNGG 2 cut(s) 869, 1922
SduI GDGCHC 4 cut(s) 147, 893, 1659, 2553
SfaNI GCATC 8 cut(s) 142, 292, 1030, 1591, 2164, 2473, 2982, 3016
SfcI CTRYAG 2 cut(s) 1725, 2879
SfoI GGCGCC 1 cut(s) 2509
Sfr274I CTCGAG 1 cut(s) 146
SlaI CTCGAG 1 cut(s) 146
SmiMI CAYNNNNRTG 1 cut(s) 2160
SmlI CTYRAG 5 cut(s) 29, 146, 759, 2149, 2542
SmoI CTYRAG 5 cut(s) 29, 146, 759, 2149, 2542
SnaBI TACGTA 1 cut(s) 1712
SsiI CCGC 6 cut(s) 118, 1465, 2348, 2462, 2505, 2568
SspDI GGCGCC 1 cut(s) 2507
SspI AATATT 4 cut(s) 1559, 1742, 1758, 2791
SspMI CTAG 6 cut(s) 969, 1605, 1968, 2183, 2228, 3044
StyD4I CCNGG 2 cut(s) 867, 1920
StyI CCWWGG 1 cut(s) 1566
TaaI ACNGT 4 cut(s) 184, 1070, 1226, 1771
TaiI ACGT 6 cut(s) 24, 193, 237, 934, 1315, 1714
TaqII GACCGA 1 cut(s) 533
TatI WGTACW 4 cut(s) 10, 1353, 1540, 1771
TauI GCSGC 3 cut(s) 2464, 2508, 2571
TfiI GAWTC 6 cut(s) 1198, 1730, 2113, 2417, 2477, 2615
TscAI CASTG 3 cut(s) 91, 1075, 2856
TseFI GTSAC 4 cut(s) 82, 169, 961, 3001
TseI GCWGC 6 cut(s) 580, 1157, 1926, 2070, 2357, 2360
Tsp45I GTSAC 4 cut(s) 82, 169, 961, 3001
TspGWI ACGGA 1 cut(s) 188
TspRI CASTG 3 cut(s) 91, 1075, 2856
Tth111I GACNNNGTC 1 cut(s) 59
Van91I CCANNNNNTGG 1 cut(s) 2252
VneI GTGCAC 1 cut(s) 1655
VspI ATTAAT 1 cut(s) 2003
XagI CCTNNNNNAGG 3 cut(s) 56, 630, 2592
XapI RAATTY 4 cut(s) 1248, 1527, 1836, 1980
XbaI TCTAGA 1 cut(s) 3043
XcmI CCANNNNNNNNNTGG 1 cut(s) 2746
XhoI CTCGAG 1 cut(s) 146
XmnI GAANNNNTTC 1 cut(s) 132
XspI CTAG 6 cut(s) 969, 1605, 1968, 2183, 2228, 3044
ZrmI AGTACT 1 cut(s) 12
Zsp2I ATGCAT 1 cut(s) 1045
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.